Veterinary dermatology最新文献

{"title":"Clinical features, surgical management and outcome of squamous and basal cell carcinoma in squamates and chelonians.","authors":"Ferran Solanes-Vilanova, Koen Chiers, Javier Gil-Lianes, Tom Hellebuyck","doi":"10.1111/vde.13282","DOIUrl":"10.1111/vde.13282","url":null,"abstract":"<p><strong>Background: </strong>Squamous cell carcinoma (SCC) is one of the most commonly diagnosed neoplastic disorders in reptiles. Recently, however, it has been demonstrated that basal cell carcinomas (BCCs) are frequently misclassified as SCCs. Several histological SCC and BCC variants have been characterised and their classification may allow the establishment of appropriate prognosis estimation and treatment approaches.</p><p><strong>Hypothesis/objectives: </strong>To describe the clinical features and surgical outcomes of SCCs and BCCs diagnosed between 2010 and 2022 in reptiles.</p><p><strong>Animals: </strong>Thirty-three captive reptiles (21 squamates and 12 chelonians).</p><p><strong>Materials and methods: </strong>Detailed clinical history, including staging and surgical outcomes, were performed. Statistical analysis assessed significant factors using Prism (v8.2.1).</p><p><strong>Results: </strong>While SCC was predominantly diagnosed in lizards, BCC was most commonly diagnosed in chelonians, and both neoplasms mainly occurred in adult to aged, male individuals. Although the gross pathological findings were highly comparable between SCC and BCC, considerable variation could be seen according to the primary location (oral, cutaneous or epidermis of the shell). Humane euthanasia or noncurative intent surgeries were performed in a minority of the cases. Curative intent surgeries were successful in 19 of 27 cases during a 1- to 7-year follow-up period, yet recurrence was seen in 8 cases. The results of this study allowed the identification of significant high-risk prognostic factors for SCC and BCC in reptiles.</p><p><strong>Conclusions and clinical relevance: </strong>This study contributes to predicting the clinical behaviour and prognosis of distinct SCC and BCC histological variants, and selecting the most appropriate treatment protocol.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"626-640"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-lasting otic solution containing mometasone furoate can influence intradermal testing in dogs with healthy ears and otitis externa.","authors":"Jennifer L Clegg, Clarissa P Souza, William E Sander","doi":"10.1111/vde.13281","DOIUrl":"10.1111/vde.13281","url":null,"abstract":"<p><strong>Background: </strong>Topical therapy is preferred for otitis externa (OE) in dogs. Otic products commonly contain glucocorticoids that can be systemically absorbed and possibly interfere with diagnostic tests such as intradermal testing (IDT).</p><p><strong>Hypothesis/objectives: </strong>To determine the effect of a long-lasting otic solution containing mometasone furoate (MF) on IDT immediate reactions and to determine withdrawal time before IDT.</p><p><strong>Animals: </strong>Seventeen dogs with healthy ears (Group 1) and 12 with OE (Group 2).</p><p><strong>Materials and methods: </strong>Intradermal injections of histamine, anticanine-immunoglobulin (Ig)E and saline were subjectively and objectively evaluated before treatment and weekly for 6 weeks, after a single application of a long-lasting otic solution containing MF.</p><p><strong>Results: </strong>Statistically significant reductions in the mean subjective histamine scores after application of MF were observed at Weeks 1 (p = 0.012) and 2 (p = 0.028) in Group 1, and Weeks 1 (p = 0.00066), 2 (p = 0.02) and 4 (p = 0.034) in Group 2. Mean histamine objective scores were significantly reduced in Weeks 1 (p = 0.042), 2 (p = 0.0009), 3 (p = 0.001) and 5 (p = 0.018) in Group 1 only. The mean subjective anticanine-IgE scores were significantly reduced in Week 1 in both groups (p = 0.003, p = 0.0066), respectively. There were no significant changes in the mean anticanine-IgE objective score in either group.</p><p><strong>Conclusions and clinical relevance: </strong>Application of a long-lasting otic solution containing MF can interfere with IDT immediate reactions in healthy and OE dogs. Based on the subjective evaluation, IDT can be performed ≥2 weeks after application of the otic solution in healthy dogs, and ≥4 weeks in dogs with OE.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"736-744"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141735083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical stability and in vitro antimicrobial efficacy of diluted silver sulfadiazine powder and cream over a six-month period.","authors":"Christopher M Lopresto, Brian S Palmeiro, Stephen D Cole, Xin Xu, Jaclyn Dietrich, Darko Stefanovski","doi":"10.1111/vde.13289","DOIUrl":"10.1111/vde.13289","url":null,"abstract":"<p><strong>Background: </strong>Silver sulfadiazine (SSD) is commonly formulated into otic preparations to treat otitis externa, although evidence of stability and antimicrobial efficacy with long-term storage is lacking.</p><p><strong>Objectives: </strong>To evaluate the effect of storage time on chemical stability and in vitro antimicrobial activity of SSD diluted in sterile water, including two 1% suspensions using SSD pharmaceutical-grade powder stored at room temperature (RT) in plastic or sterile glass bottles, and a 1:9 dilution using prescription SSD 1% cream stored at RT in a sterile glass bottle.</p><p><strong>Materials and methods: </strong>Liquid chromatography-tandem mass spectrometry (LC-MS/MS) assessed chemical stability. Trimethoprim/sulfamethoxazole-susceptible and trimethoprim/sulfamethoxazole-resistant strains of Staphylococcus pseudintermedius (SP), meticillin-resistant (MR) SP, S. schleiferi (SS), MRSS, Pseudomonas aeruginosa, Proteus mirabilis and Escherichia coli evaluated by 24 h time-kill analysis assessed in vitro antimicrobial efficacy. Each assessment was performed at zero, one, three and six months of storage.</p><p><strong>Results: </strong>LC-MS/MS showed no significant change in concentration over time for any suspension. When adjusted for time and species/strain, all SSD suspensions showed significant reductions in colony forming units (cfu)/mL at 24 h (p < 0.001). Including all suspensions, a bactericidal effect (minimum 3-log cfu/mL reduction at 24 h) occurred against 94% of total isolates, with failure against 33 of 552 isolates (6%). Bactericidal failure was more likely with the cream-based suspension (p < 0.05) and at six months (p < 0.01).</p><p><strong>Conclusions and clinical relevance: </strong>Powder-based and cream-based SSD/sterile water suspensions showed no significant change in concentration and demonstrated in vitro antimicrobial activity for six months. Bactericidal failure was more likely with the cream-based suspension and after six months of storage.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"704-715"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of cross-reactive allergens between the Dermatophagoides farinae house dust mite and the Toxocara canis nematode in dogs with suspected allergies.","authors":"Thierry Olivry, Ana Mas-Fontao, Sandrine Jacquenet, Martina Aumayr, Toshihiro Tsukui, Véronique Gomord, Loïc Faye, Claude Favrot","doi":"10.1111/vde.13295","DOIUrl":"10.1111/vde.13295","url":null,"abstract":"<p><strong>Background: </strong>Immunoglobulin (Ig)E cross-reactivity has been shown between Dermatophagoides farinae (Df; house dust mite) and the nematode Toxocara canis (Tc), yet its allergen basis is unknown.</p><p><strong>Objectives: </strong>To identify the Df allergens IgE-cross-reactive with those of Tc.</p><p><strong>Animals: </strong>Archived sera from 73 dogs with suspected allergy sensitised to Df.</p><p><strong>Materials and methods: </strong>We performed a combination of Pet Allergy Xplorer (PAX) and enzyme-linked immunosorbent assay (ELISA) inhibitions with excretory-secretory and somatic (i.e. nematode body) extracts of Tc or recombinant Tc tropomyosin on coats of Df, Der f 15 and Zen-1 (ELISA) or PAX allergens.</p><p><strong>Results: </strong>The ELISA and PAX inhibitions established that there is mutual yet variable cross-reactivity between the Tc excretory-secretory extract, purified Der f 15 and purified Zen-1. This cross-reactivity is likely to involve cross-reactive glycans, as there is no inhibition between the Tc excretory-secretory extract and recombinant Der f 15 without its predicted natural O-glycans. We also confirmed a heterogeneous cross-reactivity between the somatic Tc extract and Der p 11 (paramyosin), as well as between the recombinant Toxo c 3 and Der p 10 tropomyosins. The cross-reactivity among tropomyosins and paramyosins is likely to involve peptidic epitopes, as these recombinant allergens are not glycosylated.</p><p><strong>Conclusions and clinical relevance: </strong>In dogs with suspected allergies, the cross-reactivity between Tc and Df for dogs is complex and heterogeneous. Some of the cross-reactive IgE recognises shared glycans on Der f 15 and Zen-1, while some targets peptidic epitopes on shared paramyosins and tropomyosins. We do not exclude that additional cross-reactive allergens between Df and Tc also might exist.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"662-671"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of moderate-to-severe canine atopic dermatitis with modified-release mycophenolate (OKV-1001): A pilot open-label, single-arm multicentric clinical trial.","authors":"Michael Klotsman, Wayne H Anderson, Danielle Wyatt, Tom Lewis, Natalie Theus, Domenico Santoro","doi":"10.1111/vde.13283","DOIUrl":"10.1111/vde.13283","url":null,"abstract":"<p><strong>Background: </strong>Mycophenolate is an immunomodulating agent successfully used for the treatment of moderate-to-severe atopic dermatitis (AD) in people. Mycophenolate is an effective steroid-sparing treatment option for use in dogs with inflammatory skin diseases.</p><p><strong>Objective: </strong>To evaluate whether once-daily modified-release mycophenolate (OKV-1001) is safe and effective for treating moderate-to-severe canine AD.</p><p><strong>Animals: </strong>Client-owned atopic dogs (n = 9) were enrolled.</p><p><strong>Materials and methods: </strong>In an open-label multicentre pilot study, OKV-1001 (30 mg/kg every 24 h) was given orally for ≤84 days. Concomitant tapering doses of glucocorticoids were administered up to Day (D)28. Clinicians assessed Canine Atopic Dermatitis Extent and Severity Index, 4th iteration (CADESI-04) on D0, D14, D28, D56 and D84. Body weight and clinical pathological parameters were measured at baseline and at the end of the study.</p><p><strong>Results: </strong>Treatment with OKV-1001 combined with glucocorticoids significantly reduced the severity of AD within two weeks in seven of nine (77.8%) dogs. The mean percentage change from baseline in the CADESI-04 score was 29% (p = 0.009) at D14 (n = 9), 39% (p = 0.008) at D28 (n = 9) and 49% (p = 0.03) at D56 (n = 7) at which point glucocorticoids had been withdrawn. In two dogs the improvement in CADESI-04 was 62% and 23% (respectively) on D84. No significant adverse events including clinical pathological findings were reported.</p><p><strong>Conclusions and clinical relevance: </strong>Modified-release mycophenolate (OKV-1001) may represent a promising alternative treatment option for dogs with moderate-to-severe AD. The safety and efficacy profile of OKV-1001 will need to be established in larger, placebo-controlled clinical trials.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"652-661"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid induction of allergen-blocking IgG in dogs vaccinated with plant-based, Der f 2-expressing bioparticles.","authors":"Thierry Olivry, Lucie Mirande, Lorenz Aglas, Bertrand Morel, Ana Mas-Fontao, Anne-Catherine Fitchette, Lena Holztrattner, Maria Stigler, Joannie Roberge, Caroline Martel, Virginie Stordeur, Réjean Desgagnés, Louis Vézina, Claude Favrot, Véronique Gomord","doi":"10.1111/vde.13291","DOIUrl":"10.1111/vde.13291","url":null,"abstract":"<p><strong>Background: </strong>Allergen-carrying virus-like particles are effective and safe means of allergen immunotherapy (AIT) in rodent models.</p><p><strong>Objective: </strong>To study the development of allergen-blocking immunoglobulin (Ig)G in dogs injected with Der f 2-carrying enveloped plant-based bioparticles (eBPs).</p><p><strong>Materials and methods: </strong>Laboratory beagle dogs were injected intradermally (ID) or subcutaneously (SC) with Der f 2-eBP three times at 2-week intervals. A basophil mediator release assay was used to compare the reactivity of Der f 2-eBPs to that of recombinant Der f 2. Allergen-specific IgG serum levels were determined by immunoblotting and ELISA. The allergen-blocking potential of postvaccination IgG was assessed by Pet Allergy Xplorer (PAX) macroarray and basophil mediator release inhibition assays.</p><p><strong>Results: </strong>The amount of Der f 2 eBPs needed to induce basophil activation was 1000-fold higher than that of the soluble natural allergen. In both immunisation groups, eBP injections caused no adverse events and induced Der f 2-specific IgG, first detected on Day (D)14 and peaking on D41. The co-incubation of sera with a Der f 2-IgE-rich canine serum pool resulted in a mean PAX inhibition of 70% (ID) to 80% (SC) on D41. For both groups, the inhibition of basophil mediator release reached 75% on D28 and D41. The percentage inhibition of PAX and mediator release correlated significantly with Der f 2 IgG levels.</p><p><strong>Conclusion and clinical relevance: </strong>Intradermal and subcutaneous injections of Der f 2-eBPs were safe and increased Der f 2-specific IgG. The clinical benefit of immunotherapy will be evaluated in future trials enrolling atopic dogs allergic to house dust mites.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"672-682"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A prospective study evaluating the adhesive tape impression for the diagnosis of dermatophytosis in dogs and cats.","authors":"Courtney B Ludwig, Sophie A Tyler, Tatiana Lima, Linda J Vogelnest","doi":"10.1111/vde.13284","DOIUrl":"10.1111/vde.13284","url":null,"abstract":"<p><strong>Background: </strong>Potential contagion and zoonotic risk make rapid diagnosis of dermatophytosis important amongst companion animals.</p><p><strong>Hypothesis/objectives: </strong>To compare the adhesive tape impression (ATI), trichogram, Wood's lamp (WL) examination and fungal culture for diagnosis of dermatophytosis, and the ATI and trichogram for ease of use.</p><p><strong>Animals: </strong>Five dogs and 15 cats with consistent lesions, history and at least two positive dermatophyte test results.</p><p><strong>Materials and methods: </strong>After WL examination, one representative lesion per patient was sampled for ATI, trichogram and fungal culture. Microscopic detection of fungal elements on ATI and trichogram were objectively and subjectively compared. The ability of all tests to correctly detect dermatophytosis, and the ability of ATI and trichogram to detect fungal elements in >20 high-powered fields (HPF) were compared using Fisher's exact tests.</p><p><strong>Results: </strong>ATI, trichogram, fungal culture and WL were positive in 100% (20 of 20), 90% (18 of 20), 95% (19 of 20) and 65% (13 of 20) of patients, respectively, with a significant difference between WL and ATI (p = 0.0499). Fungal culture revealed Microsporum. canis (n = 16), Nannizzia gypsea (n = 1), Trichophyton mentagrophytes (n = 1) and T. rubrum (n = 1). Fungal elements were detected in >20 HPF more frequently with ATI (18 of 20) than trichogram (11 of 20) (p = 0.017), and detection required careful scanning to distinguish from background debris more often with trichogram. Fungal elements were located with neutrophil clusters and keratinocyte rafts in addition to abnormal hair fragments more frequently on ATI.</p><p><strong>Conclusions and clinical relevance: </strong>ATI had the highest ability to correctly diagnose dermatophytosis, and increased ease of detecting fungal elements compared to trichogram.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"694-703"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142000767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a direct lymphocyte proliferation test for the diagnosis of canine food allergies with delayed reactions after oral food challenge.","authors":"Carlos Fernandez-Lozano, Ana Mas-Fontao, Silvia T Auxilia, Marie Welters, Alla Olivri, Ralf S Mueller, Thierry Olivry","doi":"10.1111/vde.13312","DOIUrl":"https://doi.org/10.1111/vde.13312","url":null,"abstract":"<p><strong>Background: </strong>In humans, food allergies (FAs) are divided into those with immunoglobulin (Ig) E-mediated (immediate FA), cell-mediated (delayed FA) or both mechanisms (mixed FA). In dogs, lymphocyte stimulation tests have the highest concordance with oral food challenges (OFCs).</p><p><strong>Objectives: </strong>To report the evaluation of a lymphocyte proliferation test (LPT) in dogs with FA and delayed reactions (≥6 h) after OFC.</p><p><strong>Animals: </strong>Thirty-five healthy and 28 dogs with delayed FA.</p><p><strong>Materials and methods: </strong>Peripheral blood mononuclear cells (PBMCs) were isolated and automatically counted before and after a 5-day culture with food allergens. Stimulation indices (SIs) were then calculated. Food allergen-specific IgE was quantified using the Pet Allergy Xplorer (PAX).</p><p><strong>Results: </strong>None of the 10 healthy laboratory beagles and 25 healthy pet dogs had an SI greater than the 3.0 cut-off, indicating a specificity of 100%. All 28 dogs with delayed FA had at least one positive stimulation to a food item that induced delayed flares after OFC; the sensitivity of this LPT for the identification of delayed canine FA was 100%. The LPT correctly identified 57 of 68 food items causing delayed flares after OFC (84%). The PAX was negative for food-specific IgE in 18 of the 28 dogs (64%), as expected for delayed FA. In three dogs (11%), PAX results overlapped with those of the LPT, suggesting a mixed FA.</p><p><strong>Conclusions and clinical relevance: </strong>Food allergies with delayed reactions after OFC-those suspected of having a cell-mediated mechanism-seemed to be the most common type of FA in the studied dogs. The LPT was helpful in identifying such cases.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142682513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epicutaneous house dust mite (HDM)-induced skin lesions feature early activation of T helper 2 inflammatory and pruritogenic pathways in HDM-nonsensitised dogs.","authors":"Frane Banovic, Amanda Blubaugh","doi":"10.1111/vde.13307","DOIUrl":"https://doi.org/10.1111/vde.13307","url":null,"abstract":"<p><strong>Background: </strong>Epicutaneously house dust mite-sensitised (HDM-S) healthy dogs are commonly used as canine atopic dermatitis (cAD) models; however, the exact mechanisms of HDM-induced AD immune activation in HDM-S and HDM-nonsensitised (NS) dogs remain unclear.</p><p><strong>Objectives: </strong>To characterise the inflammatory and pruritogenic transcriptome of acute epicutaneous HDM-induced skin lesions at 6 h and 24 h in HDM-NS and HDM-S dogs; untreated skin at 0 h from each dog served as control.</p><p><strong>Animals: </strong>Six HDM-S and six HDM-NS laboratory beagles.</p><p><strong>Materials and methods: </strong>Processed expression data from GEO deposited by Schamber et al. (G3 (Bethesda), 2014, 4 and 1787) (GSE58442) were downloaded and analysed using R and the Bioconductor package. Significance analysis was performed with the limma package; genes with false discovery rate <0.05 and fold-change ≤/≥1.5 were considered significantly differentially expressed (DEGs).</p><p><strong>Results: </strong>A 2D principal component analysis revealed no clear separation between HDM-NS and HDM-S dogs at 6 h and 24 h time points. HDM-induced skin lesions in sensitised and nonsensitised dogs at the 24 h time point showed significant upregulation of T helper cell (Th)2 genes (interleukin [IL]-4R, IL-5, IL-13, CCL13 and CCL17), as well as proinflammatory- (LTB, IL-1A and IL-18), Th1- (CXCL10, OASL and MX-1) and Th17-related markers (IL-17B, IL-17F, CCL19 and CCL20). The key Th22-related maker, IL-22, was upregulated only in the HDM-S group at the 24 h time point. Both groups at 24 h featured significant upregulation of several noncytokine pruritogens, such as trypsin, chymase, cathepsin S, periostin and neuromedin B.</p><p><strong>Conclusions and clinical relevance: </strong>Taken together, we establish that epicutaneous HDM patch application induces immune changes in HDM-NS dogs with Th2 dominance and activates several itch-promoting pathways.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uveodermatological syndrome associated with alopecia areata in a one-year-old female spayed Cavalier King Charles Spaniel dog.","authors":"Barbara G McMahill, Sophie Gilbert, Jamie Haddad, Janelle Novak, Maria Shank, Verena K Affolter","doi":"10.1111/vde.13303","DOIUrl":"https://doi.org/10.1111/vde.13303","url":null,"abstract":"<p><p>Uveodermatological syndrome and alopecia areata are autoimmune disorders causing ocular and dermatological inflammation and alopecia, respectively, in dogs. This is the first report to document concurrent development of the two diseases in a dog, as has been reported in human patients. Clinical presentation and histopathological diagnosis, treatment and clinical follow-up are described.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}