Veterinary dermatology最新文献

{"title":"Evaluation of One-Time Oral Oclacitinib Administration on Pruritic Behaviours After Intradermal Interleukin-31-Induced Pruritus Injections (\"Reactive\" Model) in Healthy Dogs: A Blinded, Randomised, Cross-Over Study.","authors":"Renato Leon, Haley Starr, Frane Banovic","doi":"10.1111/vde.70010","DOIUrl":"https://doi.org/10.1111/vde.70010","url":null,"abstract":"<p><strong>Background: </strong>Administration of interleukin (IL)-31 to healthy dogs has been used in preclinical drug testing to evaluate the antipruritic effect of novel medications through a \"preventative\" design approach (i.e., drugs are given before IL-31 administration).</p><p><strong>Hypothesis/objectives: </strong>Develop and validate a \"reactive\" intradermal IL-31-induced pruritus model in healthy dogs by administering oral oclacitinib.</p><p><strong>Animals: </strong>Eight adult, healthy research-bred beagles.</p><p><strong>Materials and methods: </strong>A blinded, randomised, cross-over study. All dogs received either intradermal recombinant canine IL-31 with or without a single dose of oral oclacitinib given afterward; cross-over treatment was administered following a 4-week washout period.</p><p><strong>Results: </strong>Oclacitinib reduced the total (p = 0.0252) and local (p = 0.0078) pruritic behaviour seconds after intradermal IL-31 injections. It also reduced the total seconds of scratching (p = 0.0078), chewing/biting (p = 0.0078) and head-shaking (p = 0.0255) behaviours. No significant reduction in licking was observed. Decreases in total pruritic seconds in this \"reactive\" model following oclacitinib administration were observed at 120-180 min (p = 0.0058), 180-240 min (p = 0.0075) and 240-300 min (p = 0.0241). Likewise, decreases in local pruritic behaviour seconds were observed at 60-120 min (p = 0.0498) and 240-300 min (p = 0.0343).</p><p><strong>Conclusions and clinical relevance: </strong>The study established the first \"reactive\" canine intradermal IL-31 itch model in healthy dogs. One-time oral administration of oclacitinib significantly reduced the incidence of pruritic behaviours. Novel antipruritic medications can be assessed and compared using this \"reactive\" model in future preclinical trials.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144733535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Stability of Ceftazidime Compounded in Saline, Glycerin and Dexamethasone-SP Solutions Stored at -20°C, 4°C and 25°C Over a 60 Day Period.","authors":"McKenna Snidow, Jeremy Bachtel, Sarrah Hoppers, Krista Banks, Daniel Gustafson, Joshua B Daniels, Will Tsai","doi":"10.1111/vde.70005","DOIUrl":"https://doi.org/10.1111/vde.70005","url":null,"abstract":"<p><strong>Background: </strong>Chronic cases of canine otitis externa (OE) often develop infections with Pseudomonas aeruginosa (PA). Given the organism's high level of resistance, veterinary surgeons often turn to compounded solutions. Limited data describing the stability and potency of compounded ceftazidime (CAZ) solutions are available, which may affect clinical outcome.</p><p><strong>Hypothesis/objectives: </strong>To evaluate the chemical stability of compounded glycerin (GLY) and dexamethasone sodium phosphate (DEX-SP) CAZ solutions in three different storage temperatures over a 60-day period. Based on previous evaluations, CAZ concentrations would decrease with increased temperature and time.</p><p><strong>Materials and methods: </strong>Ceftazidime was compounded at 10 mg/mL with 100 mL 0.9% sodium chloride (NA + CAZ), 100 mL glycerin +0.9% sodium chloride (GLY + CAZ) and 100 mL dexamethasone sodium phosphate +0.9% sodium chloride (DEX-SP + CAZ), stored at -20°C, 4°C and 25°C for 60 days. Mass spectrometry was used to analyse CAZ stability at specific time points (Day[D]0, D7, D14, D28, D60).</p><p><strong>Results: </strong>Chemical stability of CAZ concentrations was affected by storage time, temperature and diluent. CAZ concentrations decreased over time with increased temperature; frozen CAZ concentrations remained stable over time for all solutions.</p><p><strong>Conclusions and clinical relevance: </strong>Compounded CAZ stability varies by diluent, storage temperature and storage duration. NA + CAZ and DEX-SP + CAZ solutions are stable for ≤ 28 days refrigerated and retain potency for ≥ 60 days if stored frozen. These solutions offer alternative options for treatment of PA OE.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement Between Pre-Consultation Client Filled History Questionnaire Responses and Verbal History During a Veterinary Dermatology Consultation.","authors":"Jyothi Surendran, Andrew Rosenberg, Craig Griffin","doi":"10.1111/vde.70004","DOIUrl":"https://doi.org/10.1111/vde.70004","url":null,"abstract":"<p><strong>Background: </strong>Pre-consultation client-completed history questionnaires are used in veterinary dermatology to improve consultation efficiency. The agreement between such a questionnaire and verbal history has not been evaluated.</p><p><strong>Objectives: </strong>To evaluate the level of agreement between responses on a pre-consultation client-completed history questionnaire and the verbal history obtained by multiple clinicians during an initial veterinary dermatology consultation.</p><p><strong>Materials and methods: </strong>A cross-sectional study was conducted, enrolling 130 canine cases by 7 clinicians from 4 different veterinary dermatology specialty clinics. A 16-question online questionnaire was completed by the client. Agreement between the client-completed questionnaire responses and verbal history regarding the same questions obtained by the clinician was determined using a 95% confidence interval. A nonvalidated scoring system categorised agreement as excellent (≥ 90% agreement) and nonexcellent (< 90% agreement). Agreement between clinicians for all questions was calculated using chi-square tests or, if any expected cell counts were < 5, a Fisher's exact test. A significance level of p < 0.05 was used.</p><p><strong>Results: </strong>One of the 16 questions showed excellent and the remaining questions non-excellent agreement. There was significant variation in agreement between clinicians for all questions (p < 0.001).</p><p><strong>Conclusions and clinical relevance: </strong>This study revealed varying levels of agreement between questionnaire responses and verbal histories. Multiple questions on the questionnaire may require modification, with most or all requiring further verification during the verbal history. Although questionnaires are useful, they cannot replace thorough verbal history-taking. The observed interclinician variability suggests that clinicians are likely to influence the accuracy and utility of pre-consultation questionnaires.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Gabapentin Administered With Prednisolone, Ciclosporin or a Placebo on Clinical Outcomes and Motor Activity in Cats With Atopic Skin Syndrome: A Prospective, Blinded, Placebo-Controlled Study.","authors":"Jeanne Morency, Éric Troncy, Aliénor Delsart, Colombe Otis, Jérôme R E Del Castillo, Andrea Gonzales, Jason Ross, Maxim Moreau, Jean-Pierre Pelletier, Johanne Martel-Pelletier, Frédéric Sauvé","doi":"10.1111/vde.70000","DOIUrl":"https://doi.org/10.1111/vde.70000","url":null,"abstract":"<p><strong>Background: </strong>Gabapentin reportedly decreases central sensitisation, a disorder associated with chronic pruritus in humans, although this is not well documented in cats. Its combined use with the standard antipruritic therapy for feline atopic skin syndrome (FASS) is not yet described.</p><p><strong>Objectives: </strong>To evaluate the impact of prednisolone, ciclosporin or placebo, with or without gabapentin, on lesional scores and actimetry in FASS cats.</p><p><strong>Animals: </strong>Twenty-six cats from a laboratory colony with naturally acquired FASS.</p><p><strong>Methods and materials: </strong>Following a 12-week washout period, cats were allocated to one of three groups: prednisolone (1 mg/kg, n = 9), ciclosporin (7 mg/kg, n = 8) and placebo (Avicel, n = 9). Treatments were administered orally, once daily for 5 weeks (Week [W]0 to W4), then combined with gabapentin (10-15 mg/kg) for another 3 weeks. The Feline Dermatitis Extent and Severity Index (FeDESI) was assessed at baseline and W2, W4 and W7. Actimetry was recorded and analysed over weekend (WE) time points. A repeated-measures generalised mixed model was applied using the zero-inflated negative binomial (FeDESI) or log-normal (actimetry) distribution (α = 0.05).</p><p><strong>Results: </strong>Prednisolone alone significantly improved FeDESI (p = 0.008), while ciclosporin required the addition of gabapentin to achieve a significant effect (p < 0.034). Gabapentin decreased FeDESI scores in all groups (p < 0.001) and demonstrated the highest incidence rate ratio (2.59) compared to placebo. Improvements in FeDESI were associated with significant (corresponding in intensity) decreases in motor activity.</p><p><strong>Conclusions and clinical relevance: </strong>Gabapentin, particularly when combined with prednisolone or ciclosporin, may reduce lesional score and actimetry-assessed itch in FASS cats, suggesting a potential central sensitisation in some cats.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Milk of Calcium in a Dog: A Rare Presentation of Calcinosis Circumscripta.","authors":"Michela De Lucia, Paola Orlandini, Francesco Godizzi, Arianna Costa, Andrea Pratesi","doi":"10.1111/vde.70002","DOIUrl":"https://doi.org/10.1111/vde.70002","url":null,"abstract":"<p><p>This report presents the clinical features, clinicopathological changes, computed tomography images and response-to-treatment of a dog with calcium-laden subcutaneous fluid collections, resembling a rare form of calcinosis in humans termed 'milk of calcium'.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppurative Malassezia Otitis Externa: Clinical Features, Treatment, and Preliminary Isolate Identification.","authors":"Danielle Nolitt, Elizabeth Drake","doi":"10.1111/vde.13372","DOIUrl":"https://doi.org/10.1111/vde.13372","url":null,"abstract":"<p><strong>Background: </strong>Suppurative Malassezia otitis externa (SMO) is a rare, severe presentation of Malassezia otitis (MO) sparsely reported in the literature.</p><p><strong>Hypothesis/objectives: </strong>The primary objective of this study was to describe the clinical presentation, diagnostic findings, treatment, and prognosis of SMO. A secondary objective was to speciate available SMO isolates, as this phenotype may be caused by different Malassezia species.</p><p><strong>Animals: </strong>Nine client-owned dogs with SMO.</p><p><strong>Materials and methods: </strong>A retrospective study of medical records from nine dogs with SMO diagnosed at an academic referral hospital from 2022 to 2024. Three SMO and 10 MO isolates from poodle-crosses were speciated with matrix-assisted laser desorption and ionisation time-of-flight mass spectrometry (MALDI-TOF MS).</p><p><strong>Results: </strong>Young (average 1-year-old), male (67%), poodle or poodle-crosses (67%; six of nine dogs) were most often affected. Clinical presentation included unilateral otitis externa (77%; seven of nine dogs) with dark brown mucoid discharge, canal ulceration, erythema and glandular hyperplastic changes (100%). Cytological findings included yeast, neutrophils, and extracellular material suggestive of biofilm (100%). Prior otic treatment was primarily misdirected towards Pseudomonas otitis. Malassezia pachydermatis was identified in two SMO isolates and half of the MO isolates. Prednisone (0.5-1 mg/kg/day) per os daily tapered over 1 month plus daily otic orbifloxacin, mometasone furoate monohydrate and posaconazole (Posatex; Merck Animal Health) achieved complete resolution in 83% of affected ears without an anaesthetised otic lavage. At the time of writing, there was no known recurrence of any case within at least 6 months of treatment.</p><p><strong>Conclusions and clinical relevance: </strong>SMO is a phenotypically severe presentation of MO clinically mimicking suppurative bacterial otitis. Young, male poodle-crosses are commonly affected. Ear canal hair plucking is likely to incite severe primary inflammation before SMO development.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Professor Ton Willemse.","authors":"Chiara Noli, Annette van der Lee, Yvette Schlotter","doi":"10.1111/vde.13371","DOIUrl":"https://doi.org/10.1111/vde.13371","url":null,"abstract":"","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2025 Selected Abstracts for NAVDF.","authors":"","doi":"10.1111/vde.13359","DOIUrl":"https://doi.org/10.1111/vde.13359","url":null,"abstract":"","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal heterogeneity and its association with skin lesions in canine epitheliotropic cutaneous T-cell lymphoma.","authors":"Toshitaka Kanei, Mami Murakami, Munetaka Iwata, Hiroaki Kamishina, Sadatoshi Maeda","doi":"10.1111/vde.13343","DOIUrl":"https://doi.org/10.1111/vde.13343","url":null,"abstract":"<p><strong>Background: </strong>The types of skin lesions in canine epitheliotropic cutaneous T-cell lymphoma (ECTCL) vary markedly; however, the mechanisms underlying this diversity remain unclear. Human ECTCL exhibits clonal heterogeneity, with different clones of neoplastic lymphocytes being observed in skin lesions from the same patients. Therefore, we hypothesised that diversity in skin lesions may be attributed to clonal heterogeneity.</p><p><strong>Objectives: </strong>To evaluate clonality and its association with skin lesions.</p><p><strong>Materials and methods: </strong>PCR for T-cell receptors was performed on 25 formalin-fixed paraffin-embedded lesional skin samples derived from eight ECTCL cases. A fragment analysis was performed to establish whether clonal patterns were identical or nonidentical between lesions. The associations of clonal patterns with the types and histopathological features of skin lesions were investigated by statistical analyses. A transcription analysis was also conducted to examine the expression of recombination-activating gene (RAG)1 in skin lesions.</p><p><strong>Results: </strong>The fragment analysis identified only one case with an identical clonal pattern in all skin lesions. Nonidentical clonal patterns were detected in the seven other cases. Clonal patterns were not associated with the types of skin lesions or histopathological features. The transcription analysis did not detect RAG1 in any skin lesions.</p><p><strong>Conclusions and clinical relevance: </strong>The present study is the first to report clonal heterogeneity in canine ECTCL that was not associated with the clinical or histopathological features of skin lesions. The results obtained also suggested that clonal heterogeneity originated not in the skin.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":"36 3","pages":"353-361"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ozone water has antibacterial properties in dogs without skin barrier impairment.","authors":"Akira Matsuda, Tetsuya Ano, Yukari Nakamura, Takamasa Itoi, Kiyotaka Arai, Kenji Kutara, Keisuke Sugimoto, Noritaka Maeta","doi":"10.1111/vde.13339","DOIUrl":"10.1111/vde.13339","url":null,"abstract":"<p><strong>Background: </strong>Recently, the use of disinfectants to treat bacterial skin diseases in dogs has garnered attention from the perspective of preventing the spread of antibiotic-resistant bacteria. Ozone water is a disinfectant that combines high antibacterial efficacy and safety; however, there have been no reports verifying its effects on skin barrier function in dogs.</p><p><strong>Objectives: </strong>This study aimed to verify the antibacterial properties and effects of ozone water on skin barrier function in dogs.</p><p><strong>Animals: </strong>Four beagles for the antibacterial test and six beagles for the barrier function test.</p><p><strong>Materials and methods: </strong>Purified water, 3 mg/L ozone water and 0.005% and 0.05% sodium hypochlorite were used as test products. Skin bacteria were collected 5 min after a single application of each and cultured to measure the bacterial counts. Trans-epidermal water loss (TEWL) and skin surface hydration (SSH) were measured before and after application once a day for 2 weeks. Additionally, the proliferation of canine epidermal keratinocytes (CPEK) was examined after a 5-min incubation with the test products.</p><p><strong>Results: </strong>Ozone water and 0.05% sodium hypochlorite significantly reduced the number of skin bacteria. None of the test products worsened TEWL and SSH values. Sodium hypochlorite completely inhibited the proliferation of CPEK, while ozone water did not.</p><p><strong>Conclusions and clinical relevance: </strong>Ozone water has an antibacterial effect on canine skin comparable to that of 0.05% sodium hypochlorite, but does not inhibit keratinocyte proliferation in vitro. Thus, ozone water has the potential to be used as a safe and effective disinfectant for canine skin.</p>","PeriodicalId":23599,"journal":{"name":"Veterinary dermatology","volume":" ","pages":"283-290"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143626329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}