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Bacilloscopy, Bacterial culture, and Spoligotyping of Mycobacterium bovis strains isolated from cattle in North Central Algeria 从阿尔及利亚中北部牛身上分离的牛分枝杆菌菌株的细菌学、细菌培养和孢子分型
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.5
C. Mecherouk, M. Ifticene, N. Mimoune
{"title":"Bacilloscopy, Bacterial culture, and Spoligotyping of Mycobacterium bovis strains isolated from cattle in North Central Algeria","authors":"C. Mecherouk, M. Ifticene, N. Mimoune","doi":"10.46419/vs.54.6.5","DOIUrl":"https://doi.org/10.46419/vs.54.6.5","url":null,"abstract":"This study aimed to assess bovine tuberculosis-like cases in three slaughterhouses in North Central Algeria and to confirm these suspected cases using microscopic, bacteriological examination, and molecular biology technique. We highlighted the factors influencing the prevalence of the disease. Also, the genomic profiles of Mycobacterium tuberculosis complex (MTBC) strains isolated by Oligonucleotide typing technique (Spoligotyping) were determined. At the abattoir level, bovine carcasses were routinely inspected to detect visible abnormalities including suspicious lesions of bovine tuberculosis (BTB). At the laboratory level (Pasteur Institute, Algiers), Ziehl-Neelsen staining, bacterial culture, biochemical study (nitrate reduction test), and spoligotyping were performed to confirm suspected cases. On a total of 1300 bovine carcasses, 100 presented BTB-like cases (7.69%). Animals over 5 years of age were more affected compared to other age groups. Lesions were observed more often in females than in males and cattle of local breed were the most exposed to BTB-like cases (P<0.05). Bacilloscopy data were positive for 44 of the 100 suspected samples (44%) while bacteriology showed that 56 cultures were positive, while 35 were negative and 9 were contaminated. Molecular spoligotyping of 40 Mycobacterium strains samples showed 19 spoligotype profiles of M. bovis, of which 50% of profiles have been previously detected in the Mediterranean area and the three spoligotype patterns not previously reported were named SB2651, SB2652, SB2653 (by http:// www.Mbovis.org). Measures and means to prevent TB transmission among animals and to humans should be recommended, and more intensive investigations are required using both routine and molecular diagnostic techniques to understand and further explore MTBC.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45486384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Pathophysiology of mast cell tumour in humans and animals 人和动物肥大细胞瘤的病理生理学
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.1
S. Faraguna, Romana Turk
{"title":"Pathophysiology of mast cell tumour in humans and animals","authors":"S. Faraguna, Romana Turk","doi":"10.46419/vs.54.6.1","DOIUrl":"https://doi.org/10.46419/vs.54.6.1","url":null,"abstract":"Cilj ovog preglednoga rada bio je istražiti dosadašnje spoznaje o patofiziološkim mehanizmima nastanka mastocitoma u različitih vrsta životinja i ljudi. Također je bila namjera na temelju literaturnih podataka usporediti biološko ponašanje i oblike mastocitoma u ljudi i različitih životinjskih vrsta i komparativnim pristupom istražiti najvažnija obilježja mastocitoma, njihovu pojavnost te mogućnost širenja. Patogeneza mastocitoma nije još u potpunosti razjašnjena. Pretpostavlja se da je više čimbenika uključeno u mehanizam nastanka; pasminska predispozicija upućuje na gensku komponentu uključenu u patogenezu mastocitoma, a važnu ulogu ima i mutacija gena koji kodira receptor s aktivnošću tirozin ‒ kinaze (KIT) na membrani mastocita za vezanje čimbenika matičnih stanica (SCF, engl. stem cell factor). Mutacija KIT gena uzrokuje stvaranje KIT receptora koji je kontinuirano aktivan u odsutnosti liganda, tj. SCF-a što dovodi do proliferacije mastocita i nastanka mastocitoma. Mutacije KIT gena u pasa su najčešće locirane na eksonu 11 koji kodira regulatornu jukstamembransku domenu KIT receptora, dok je u ljudi s mastocitozom najzastupljenija mutacija D816V na poziciji 2447 kodirajuće sekvence KIT gena. Mutacije KIT gena u pasa su povezane s progresijom i lošijom prognozom mastocitoma, dok u ljudi i u mačaka mutacije KIT gena nisu povezane s prognozom bolesti, premda u ljudi imaju bitnu ulogu u dijagnostici i terapiji.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42865834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Profile of maternal serum oxytocin in postpartum and non-pregnant rats 产后和非妊娠大鼠母体血清催产素的变化
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.8
B. A. Tjokroprawiro, M. I. Aldika Akbar, E. Luqman, Widjiati Widijati
{"title":"Profile of maternal serum oxytocin in postpartum and non-pregnant rats","authors":"B. A. Tjokroprawiro, M. I. Aldika Akbar, E. Luqman, Widjiati Widijati","doi":"10.46419/vs.54.6.8","DOIUrl":"https://doi.org/10.46419/vs.54.6.8","url":null,"abstract":"Oxytocin is primarly secreted in the brain as a neuromodulator that affects numerous neurophysiological and behavioral processes. It is also produced in the ovaries and uterus to stimulate delivery and lactation. Oxytocin mRNA is found in the endometrial epithelial cells of non-pregnant women during ovulation and menstruation. Until recently, there have been no data on scientific-level oxytocin in virgin female rats. This study aimed to compare the level of oxytocin in different physical biology between postpartum and non-pregnant experimental animals (virgin or had never given birth). This experimental study was conducted on 19 female white rats (Rattus norvegicus) allocated to two groups: T1 and T2. The ten rats in group T1 (nulliparous virgin) and the nine in group T2 (postpartum) were sacrificed on day two, except for group T1, which were sacrificed following vaginal delivery. Blood was collected intracardiacally, and serum oxytocin levels were evaluated using an ELISA assay. The T-test was used for statistical data analysis. The serum oxytocin level in the T2 group (628.06 ± 168.72 pg/mL) was significantly higher than in the T1 group (366.71 ± 185.03 pg/mL; P < 0.05). In conclusion, oxytocin levels were higher in postpartum animals than in virgin animals. Thus, oxytocin plays a greater role in female reproduction than in normal physiological condition.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44471756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Učinci ekstrakta Cytinus hypocistis (L.) L. u životinjskom modelu neoplazije inducirane papiloma virusom Učinci ekstrakta Cytinus hypocisis(L.)L.Uživotinjskom modelu neoplazije inducirane papiloma virusom
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.9
Catarina Medeiros, Ana Rita Silva, Ana I. Faustino-Rocha, Adelina Gama, Paula Alexandra Oliveira, Tiago Ferreira, Lillian Barros, Maria J. Neuparth, Francisco Peixoto, Margarida M. S. M. Bastos, Rui Medeiros, Rui M. Gil da Costa, Maria João Pires
{"title":"Učinci ekstrakta Cytinus hypocistis (L.) L. u životinjskom modelu neoplazije inducirane papiloma virusom","authors":"Catarina Medeiros, Ana Rita Silva, Ana I. Faustino-Rocha, Adelina Gama, Paula Alexandra Oliveira, Tiago Ferreira, Lillian Barros, Maria J. Neuparth, Francisco Peixoto, Margarida M. S. M. Bastos, Rui Medeiros, Rui M. Gil da Costa, Maria João Pires","doi":"10.46419/vs.54.6.9","DOIUrl":"https://doi.org/10.46419/vs.54.6.9","url":null,"abstract":"Infections with certain types of papillomavirus, such as the human papillomavirus 16 (HPV16), are associated with the development of preneoplastic lesions and cancers of the anogenital, and head and neck regions. Cytinus hypocistis (L.) L. extracts are composed of substances presenting antiproliferative, antioxidant, anti-inflammatory and antibacterial properties, which might be promising as new therapeuticcompounds. This study analysed the influence of topical application of an extract obtained from C. hypocistis (CH) on K14-HPV16 and FVB/n mice to evaluate its therapeutic and toxicological properties. To achieve the study goals,30 female mice, 33–37 weeks old, were divided into six groups (n=5/group): I (HPV+CH3.1); II (HPV+CH6.2); III (HPV+CH12.4); IV (FVB/n+CH12.4); V (HPV+control) and VI (FVB/n+-control). CH was applied topically to both ears for 28 days. After this period, all animals were sacrificed for samples collection. Skin lesions were classified histologically. Toxicological parameters included haematological and biochemical blood markers, and hepatic oxidative stress analysis. Transgenic animals showed a decrease in mean body weight regardless of the concentration of extract applied. The extract had no influence on physiological parameters, organ weight, or biochemical and oxidative stress parameters. Histology demonstrated the presence of proliferative epithelial lesions in the skin and oral mucosa of K14-HPV16 mice, with no association with the application of this extract. Overall, the application of CH extract had no influence on the skin lesions and was well tolerated by the animals in these concentrations.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46281898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze 巴基斯坦绵羊线粒体细胞色素b和细胞色素氧化酶I亚区序列的分子系统发育和特征
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.4
Tuba Noreen, Tanveer Hussain, Muhammad Mansha, Zinnia Mansoor, Abdul Wajid, Muhammad Rizwan Ashraf, Goher Ayub, Muneeb M. M. Musthafa, Faiz M. M. T. Marikar
{"title":"Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze","authors":"Tuba Noreen, Tanveer Hussain, Muhammad Mansha, Zinnia Mansoor, Abdul Wajid, Muhammad Rizwan Ashraf, Goher Ayub, Muneeb M. M. Musthafa, Faiz M. M. T. Marikar","doi":"10.46419/vs.54.6.4","DOIUrl":"https://doi.org/10.46419/vs.54.6.4","url":null,"abstract":"The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to Polymerase Chain Reaction (PCR) for amplification of Cytochrome b and COI genes using appropriate primers. PCR products were sequenced and analyzed by MEGA X software. The phylogeny analysis categorized Ovis aries including Mundri sheep into three and two groups for Cytochrome b and COI genes respectively. It showed Mundri sheep as a separate group and thus as a separate breed from all other local sheep breeds. Hence the study validates based on Cytochrome b and COI that Mundri sheep is a distinctive breed from the rest of the local sheep breed.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45828289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence and infestation degree of yeast in canine otitis externa 犬外耳炎中酵母菌的流行及感染程度
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.7
N. Tešin, I. Stančić, Z. Kanački, J. Spasojević, Z. Ružić, I. Galić, Z. Kovačević
{"title":"Prevalence and infestation degree of yeast in canine otitis externa","authors":"N. Tešin, I. Stančić, Z. Kanački, J. Spasojević, Z. Ružić, I. Galić, Z. Kovačević","doi":"10.46419/vs.54.6.7","DOIUrl":"https://doi.org/10.46419/vs.54.6.7","url":null,"abstract":"Otitis externa is a persistent and recurrent problem, and the resistance of the involved pathogens to conventional drugs has made this a serious issue in veterinary medicine. This is an inflammation of the external ear canal in dogs that can be caused by bacteria or yeast. Often, these two causative agents are isolated together in combination. This study aimed to determine the prevalence of yeast and the degree of infestation of the isolated pathogens in canine otitis externa at veterinary clinics for small pets at the Department of Veterinary Medicine, University of Novi Sad, Serbia. Samples were taken from 60 dogs with clinical symptoms of otitis externa using a sterile swab and sent to laboratory for further mycological and bacteriological examination. In order to isolate the bacteria and yeast, conventional methods were used under aerobic conditions. Of the total samples, 53 (88.33%) were positive for at least one of the causative agents of otitis externa. Yeasts were isolated from 36 (52.94%) samples. The study showed that in comparing mixed and independent types of infection, in most cases 15/23 (65.22%) the degree of infestation with yeast was highest when the infectionwas mixed with bacteria. In relation to dog breeds, their ear forms and type of infection, more yeast samples (either as solo or mixed infection) were isolated from dogs with pendulous ears. It has been confirmed that Malassezia pachydermatis is the most common yeast pathogen of otitis externa in dogs, compared to other species of this genus, and it occurs in a higher degree of infestation in mixed infections than in solo infections. Dog breeds with pendulous ear form are more predisposed to yeast otitis externa than erect eared dog breeds.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45922046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hranidbena i mesna svojstva mladih svinja različitog intenziteta formiranja u ranoj ontogenezi i unutarpasminske diferencijacije prema genu za melanokortin 4 (Mc4r) receptor 幼猪的食物和肉的特性在个体发生早期和在pasmin内分化为黑素皮质素4基因受体(Mc4r)时各不相同
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.10
V. Khalak, V. Voloshchuk, О. Stadnytska, B. Gutyj, Liudmyla Zasucha, A. Onyshchenko, M. Ilchenko, Nataliia Ofilenko, V. Pokhyl, Vasul Pundyk, O. Bezaltychna
{"title":"Hranidbena i mesna svojstva mladih svinja različitog intenziteta formiranja u ranoj ontogenezi i unutarpasminske diferencijacije prema genu za melanokortin 4 (Mc4r) receptor","authors":"V. Khalak, V. Voloshchuk, О. Stadnytska, B. Gutyj, Liudmyla Zasucha, A. Onyshchenko, M. Ilchenko, Nataliia Ofilenko, V. Pokhyl, Vasul Pundyk, O. Bezaltychna","doi":"10.46419/vs.54.6.10","DOIUrl":"https://doi.org/10.46419/vs.54.6.10","url":null,"abstract":"The aim of this study was to investigate young pig fattening and meat quality due to the varying formation intensities in early on- togenesis and two genotypes based on the mel- anocortin receptor 4 (Mc4r) gene. To calculate the correlations between traits and the econom- ic efficiency of using animals of experimental groups in industrial complex conditions, the assessment of animals for fattening and meat quality examined the following characteris- tics: average daily live weight gain during the control fattening period in grams; age of reaching 100 kg live weight in days, chilled car- cass length in centimetres; length of the bacon half of the cooled carcass in centimetres; lard thickness at the thoracic vertebrae T6-T7 in millimetres. Further, the biometric processing of research results and calculation of the eco- nomic efficiency was performed. Young pigs of the controlled population met the requirements of the 1st class and the elite class for fattening and meat qualities. In examining the interbreed differentiation of large white breed animals, the melanocortin 4 receptor (Mc4r) gene determined that the Mc4rAG genotype outperformed the Mc4r AA genotype for average daily live weight gain, age at live weight of 100 kg, lard thickness at T6-T7, and chilled carcass length by an average of 4.50%. The difference between groups for the Tyler B's index was 11.82 points, and according to the average daily increase in live weight, the disparity between young pigs of interbreed differentiation according to the formation intensity index was 4.69%, the age of reaching 100 kg live weight was 3.10%, and the chilled carcass length was 1.23%. The num- ber of reliable connections between fattening and meat qualities, and the formation inten- sity and Tyler B indices was 75.0%, indicating their promise for use in selection and breeding work. The maximum increase in additional production was obtained from young pigs of the Mc4r AG genotype (+2.71%) and of group I, in which the formation intensity index ranged from 0.935 to 1.087 points (+2.65%).","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48712940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lentivirus malih preživača
Veterinarska stanica Pub Date : 2023-05-31 DOI: 10.46419/vs.54.6.3
João Jacob_Ferreira, Isabel Pires, Nuno Alegria, A. Coelho, Andrea Garcês, Luis Miguel Ferrer, Delia Lacasta, Hélder Quintas
{"title":"Lentivirus malih preživača","authors":"João Jacob_Ferreira, Isabel Pires, Nuno Alegria, A. Coelho, Andrea Garcês, Luis Miguel Ferrer, Delia Lacasta, Hélder Quintas","doi":"10.46419/vs.54.6.3","DOIUrl":"https://doi.org/10.46419/vs.54.6.3","url":null,"abstract":"Small ruminant lentivirus (SRLV) is a group of viruses of the Retroviridae family, shared between caprine, ovine and wild ruminants. It is responsible for a systemic infection that can affect the lungs, central nervous system, mammary gland and joints, causing chronic, insidious, and progressive diseases, seriously affecting animal health. Concurrently, it is associated with a decrease in milk production, leading to malnutrition of lambs and goat kids and to the premature slaughter of adult animals, causing substantial economic losses. This review aims to gather the latest information regarding lentivirusin small ruminants in the clinical practice, their economic importance, and diagnostic and prevention methods. Diagnosis is based on clinical, analytical, and post-mortem findings. The feasibility of imaging diagnosis is also highlighted. Preventive measures and management interventions, including the culling or segregation of positive animals, are effective options to control or even eradicate this disease. SRLV prevention strategies must be applied continuously to progressively eradicate infection.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41436255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimikrobna osjetljivost i kretanje antimikrobne rezistencije bakterijskih uzročnika infekcija urinarnog trakta u pasa izdvojenih u Hrvatskoj u razdoblju 2012.-2022. godine 2012年至2022年间克罗地亚细菌性尿路感染引起的抗生素敏感性和耐药性变化。年
Veterinarska stanica Pub Date : 2023-03-16 DOI: 10.46419/vs.54.5.10
Gordan Kompes, Antonela Bagarić, Branka Šeol Martinec, Željko Cvetnić, Irena Reil, Sanja Duvnjak, Silvio Špičić, Luka Cvetnić, Miroslav Benić, Boris Habrun
{"title":"Antimikrobna osjetljivost i kretanje antimikrobne rezistencije bakterijskih uzročnika infekcija urinarnog trakta u pasa izdvojenih u Hrvatskoj u razdoblju 2012.-2022. godine","authors":"Gordan Kompes, Antonela Bagarić, Branka Šeol Martinec, Željko Cvetnić, Irena Reil, Sanja Duvnjak, Silvio Špičić, Luka Cvetnić, Miroslav Benić, Boris Habrun","doi":"10.46419/vs.54.5.10","DOIUrl":"https://doi.org/10.46419/vs.54.5.10","url":null,"abstract":"Bakterijske su infekcije urinarnog trakta (IUT) u pasa učestale. Porast antimikrobne rezistencije i uporaba kritično-važnih antimikrobnih lijekova, osobito u liječenju IUT pasa, zabrinjavajuća je zbog pojave multiplo rezistentnih bakterijskih sojeva. Cilj je ovog rada bio prikazati prevalenciju i antimikrobnu resistenciju najčešćih bakterijskih uzročnika IUT pasa i trendove antimikrobne rezistencije izdvojenih bakterijskih sojeva u Republici Hrvatskoj tijekom 11 godina. Od ukupno 2721 pretraženih uzoraka urina, potitivno je bilo 1363 (50,1 %). Bakterijska vrsta Escherichia coli bila je najčešće izdvojeni mikroorganizam (47,3 %), nakon čega slijede: koagulaza pozitivni Staphylococcus sp. (20,7 %), Proteus sp. (14,7 %), Enterococcus sp. (13,6 %), beta hemolitični Streptococcus sp. (11,7 %), Pseudomonas aeruginosa (4,5 %) i meticilin rezistentni koagulaza pozitivni Staphylococcus sp. (3,4 %). Kod Gram-negativnih bakterija uočen je visok stupanj osjetljivosti na: gentamicin, amikacin (77,9 %-97,3 %), marbofloksacin i ciprofloksacin (69,7 %-98,4 %). Kod bakterijskog roda Proteus utvrđen je i visok stupanj osjetljivosti na beta-laktame (70,5 %-86,7 %), a kod Escherichia coli i na kloramfenikol (73,3 %) i trimetoprim/sulfametoksazol (72,7 %). Neznatan postotak koagulaza pozitivnih Staphylococcus sp. bila je osjetljiva na penicilin (10,6 %) i ampicilin (16,7 %), dok je mala osjetljivost zamijećena na streptomicin, neomicin i tetracikline (56,0 %-66,0 %). Najmanji postotak beta hemolitičnih Streptococcus sp. bio je osjetljiv na aminoglikozide, tetracikline (8,2 %-31,9 %) i fluorokinolone (31,5 %-61,6 %). Kod bakterijskih izolata Enterococcus sp. najveća osjetljivost prisutna je na amoksicilin/klavulansku kiselinu (78,0 %) i ampicilin (70,4 %). Amikacin i kloramfenikol, s postotkom osjetljivosti od 89,3 %, odnosno 80,9 %, bili su jedini antimikrobni lijekovi učinkoviti na meticilin rezistentne koagulaza pozitivne Staphylococcus sp. Tijekom 11 godina istraživanja zamijećen je trend porasta antimikrobne rezistencije bakterija Escherichia coli i Proteus sp., izdvojenih iz IUT pasa, na fluorokinolone, ampicilin i amoksicilin/klavulansku kiselinu, dok je kod bakterijske vrste Escherichia coli uočen porast antimikrobne rezistencije i na cefalosporine. Ovi rezultati će pomoći pri empirijskom odabiru antimikrobnih lijekova za terapiju IUT pasa te podizanju svijesti o razumnoj uporabi antimikrobnih lijekova.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47625621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dairy production in the indigenous Arbia goat breed and growth performance of their kids in Algeria 阿尔及利亚当地阿尔比亚山羊品种的乳制品生产及其子女的生长表现
Veterinarska stanica Pub Date : 2023-02-06 DOI: 10.46419/vs.54.5.4
Hind Hoissou, Abir Labiod, Aya Ramdani, T. Khenenou
{"title":"Dairy production in the indigenous Arbia goat breed and growth performance of their kids in Algeria","authors":"Hind Hoissou, Abir Labiod, Aya Ramdani, T. Khenenou","doi":"10.46419/vs.54.5.4","DOIUrl":"https://doi.org/10.46419/vs.54.5.4","url":null,"abstract":"Knowledge of dairy potential is essential to create products that fully express their genetic potential. This study was carried out on 94 Arbia adult goats and 65 kids (n=195) (February – May 2022) in the wilayas of Souk-Ahras and Tebessa with the aim of estimating the dairy potential of the breed, and the daily gains (ADG) between birth and 90 days of age. The data revealed that daily milk production (n=94) was 0.89±0.85 litres per day. Milk quality was characterised by an average composition of 3.2% fat, 13.46% total protein, 17.28 % total dry extract, 0.78% salt, pH 7.08±0.01, acidity 17.7°D, density 1032 and protein/fat ratio 4.29. Regarding growth performance, the average birth weight was W0 = 1.80±0.61 kg, while the average at 90 days was W90 = 10.05±2.02 kg, which corresponds to an overall daily weight gain of ADG0-90 = 91.66 g/day. The study showed that birth weight was not affected by sex (P>0.05), though males grew faster after weaning (P<0.05), and the performance aspect carried out by some physicochemical characteristics of milk was affected by area (P<0.05). The study results indicated that the local breed goat had good production dairy performance and goat kids demonstrated acceptable potential for meat production under the semi-arid Algerian environmental conditions. Therefore, it would be suitable to check for the presence of lactogenic plants on the pastures of these breeders to enhance production.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47434077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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