Molekularna filogenija i karakterizacija mundri ovce (Ovis aries) u Pakistanu sekvenciranjem mitohondrijskog citokroma b i podjedinice I citokrom oksidaze

Q3 Veterinary

Veterinarska stanica Pub Date : 2023-05-31 DOI:10.46419/vs.54.6.4

Tuba Noreen, Tanveer Hussain, Muhammad Mansha, Zinnia Mansoor, Abdul Wajid, Muhammad Rizwan Ashraf, Goher Ayub, Muneeb M. M. Musthafa, Faiz M. M. T. Marikar

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Abstract

The main focus of this research is to determine the molecular phylogeny and characterization of Mundri Sheep (Ovis aries) through sequencing of mitochondrial Cytochrome b and Cytochrome Oxidase Subunit I (COI). This sheep breed appears morphologically different from other local sheep breeds of Pakistan. The current research is carried out to appraise the status of Mundri sheep whether it is aa different breed from other breeds or not. Blood samples of Mundri sheep were collected from Livestock Experiment Station, (LES) Fazilpur in district Rajanpur (Punjab). DNA was isolated and subjected to Polymerase Chain Reaction (PCR) for amplification of Cytochrome b and COI genes using appropriate primers. PCR products were sequenced and analyzed by MEGA X software. The phylogeny analysis categorized Ovis aries including Mundri sheep into three and two groups for Cytochrome b and COI genes respectively. It showed Mundri sheep as a separate group and thus as a separate breed from all other local sheep breeds. Hence the study validates based on Cytochrome b and COI that Mundri sheep is a distinctive breed from the rest of the local sheep breed.

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巴基斯坦绵羊线粒体细胞色素b和细胞色素氧化酶I亚区序列的分子系统发育和特征

本研究的主要重点是通过线粒体细胞色素b和细胞色素氧化酶亚基I (COI)的测序来确定蒙德里羊(Ovis aries)的分子系统发育和特征。这个羊品种在形态上与巴基斯坦其他地方羊品种不同。本研究旨在评价蒙德里羊是否与其他品种不同。蒙德里羊的血液样本采集于旁遮普Rajanpur区的Fazilpur家畜实验站。分离DNA，使用合适的引物进行聚合酶链反应(PCR)扩增细胞色素b和COI基因。PCR产物用MEGA X软件测序分析。系统发育分析将包括蒙德里羊在内的绵羊分别分为3组和2组细胞色素b和COI基因。这表明蒙德里羊是一个独立的群体，因此与所有其他地方羊品种不同。因此，基于细胞色素b和COI，本研究验证了Mundri羊是一个独特的品种，与当地其他羊品种不同。

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来源期刊

Veterinarska stanica Veterinary-Veterinary (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The goal of the journal is to provide an international platform for the publication of articles in the fields of veterinary and animal sciences, and biotechnology. The content of the journal is particularly dedicated to veterinary practitioners, but also to veterinary scientists and university professors, to encourage them to share their knowledge and experience on this platform. Manuscripts submitted to the journal may include: original scientific papers, review articles, short communications, professional articles, case reports, conference reports and literary records and reviews of new book either in Croatian or English languages.