Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho
{"title":"Razotkrivanje stanja: istraživanje prevalencije virusa mačje imunodeficijencije i virusa mačje leukemije među mačkama iz skloništa u Portugalu","authors":"Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho","doi":"10.46419/vs.55.5.1","DOIUrl":"https://doi.org/10.46419/vs.55.5.1","url":null,"abstract":"Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are important pathogens affecting feline hosts worldwide, compromising the health, well-being, quality of life, and lifespan of infected cats. This study explores the prevalence of these viral agents among shelter cats in Portugal, aiming to unravel their complexities and implications. The prevalence of FIV and FeLV was assessed by collecting blood samples (n = 326) from cats during medical routine check-ups at shelter admission and testing them with an immunochromatographic kit. The overall seroprevalence of FIV and FeLV was 15.3% (50/326, 95% confidence interval [CI]: 11.6–19.7). The proportion of cats positive to FIV, FeLV, and both FIV and FeLV was 9.8% (32/326, 95% CI: 6.8-13.6), 2.1% (7/326, 95% CI: 0.9-4.4) and 3.4% (11/326, 95% CI: 1.7-6.0), respectively. The prevalence of FIV and both FeLV and FIV appeared to be higher in the adult population. No statistical association was found between sex, breed, hair, or municipality, and FeLV, FIV and both FeLV and FIV prevalence. This is the first report on retroviral prevalence in shelter cats in Portugal, which provides important information regarding FIV and FeLV among stray cats in Portugal, highlighting the need for surveillance of those viruses, improved compliance with vaccination programmes, and vigilant management strategies to limit and control these viruses in the felinepopulation.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139782170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić
{"title":"Procjena reproduktivne uspješnosti u koza – pregled odabranih čimbenika koji na to utječu","authors":"J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić","doi":"10.46419/vs.55.5.8","DOIUrl":"https://doi.org/10.46419/vs.55.5.8","url":null,"abstract":"Dairy goats are a very successful form of livestock farming in developing countries, with functional products for human consumption, which makes their breeding attractive. Breeding efficiency is determined by successful reproductive parameters. Several parameters can be used as indicators of reproductive success, such as the number of kids born, age at first kidding, kidding interval, percentage of kids born and weaned, abortion rate, body weight of kids at birth and at weaning, and the length of the goat's reproductive life. Due to the seasonality of reproduction and multiple possibilities of controlling the sexual cycle in goats (hormonal and non-hormonal methods), it is difficult to standardise parameters that could be systematically used to detect suboptimal production, and whose improvement would increase reproductive success. Adequate housing conditions and feeding are inseparable from good breeding and productivity of animals. By meeting these measures with constant observation of reproductive success parameters, high fertility, large litters (2 kids per pregnancy) with a survival rate of 90% until weaning can be expected in goat breeding.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139840779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal
{"title":"Testing the potential antifungal activity of Origanum vulgare against Aspergillus fumigatus, Aspergillus Niger and Talaromyces marneffei isolated from pets","authors":"João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal","doi":"10.46419/vs.55.5.6","DOIUrl":"https://doi.org/10.46419/vs.55.5.6","url":null,"abstract":"Origanum vulgare (oregano) is an aromatic herb commonly used in the Mediterranean, widely known for its medicinal properties. In this experiment, we tested its antifungal properties against two species of Aspergillus (A. niger and A. fumigatus) and the emerging Talaromyces marneffei. We tested the plant’s antifungal activity in a range of concentrations (5, 10, 20 and 30 mg/mL) in which the plant was dissolved and mixed with growth medium (PDA) and then inoculated with the fungi. The percentage of inhibition was measured over 7 days with data being collected on the 3rd, 5th and 7th days. Fungal activity inhibition ranging from 30 to 100% was achieved against Aspergillus niger and Aspergillus fumigatus and from 50 to 100% against Talaromyces marneffei. This multi-disciplinary study brought the use of plants into the veterinary sciences by using samples isolated from pets, with the aim of researching potential alternatives to traditional antifungal treatments and the ultimate goal ofincreasing animal wellbeing.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić
{"title":"Procjena reproduktivne uspješnosti u koza – pregled odabranih čimbenika koji na to utječu","authors":"J. Šavorić, V. Stevanović, S. Vince, Ivona Matić, Juraj Grizelj, Martina Lojkić, Nino Maćešić, M. Samardžija, Tugomir Karadjole, Branimira Špoljarić","doi":"10.46419/vs.55.5.8","DOIUrl":"https://doi.org/10.46419/vs.55.5.8","url":null,"abstract":"Dairy goats are a very successful form of livestock farming in developing countries, with functional products for human consumption, which makes their breeding attractive. Breeding efficiency is determined by successful reproductive parameters. Several parameters can be used as indicators of reproductive success, such as the number of kids born, age at first kidding, kidding interval, percentage of kids born and weaned, abortion rate, body weight of kids at birth and at weaning, and the length of the goat's reproductive life. Due to the seasonality of reproduction and multiple possibilities of controlling the sexual cycle in goats (hormonal and non-hormonal methods), it is difficult to standardise parameters that could be systematically used to detect suboptimal production, and whose improvement would increase reproductive success. Adequate housing conditions and feeding are inseparable from good breeding and productivity of animals. By meeting these measures with constant observation of reproductive success parameters, high fertility, large litters (2 kids per pregnancy) with a survival rate of 90% until weaning can be expected in goat breeding.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139780899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune
{"title":"Reproduction mode and Strain effects on Rabbit Breeding Performances","authors":"Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune","doi":"10.46419/vs.55.5.3","DOIUrl":"https://doi.org/10.46419/vs.55.5.3","url":null,"abstract":"This study was carried out to determine the impact of the mode of reproduction and the strain (male and female) of rabbits on some zootechnical and reproduction parameters. For that, monitoring was carried out in 2019 in the region of Setif (North Eastern Algeria) on a semi-intensive breeding of 25 crossbreed rabbits. The statistical analysis showed a very highly significant effect of the mode of reproduction on the number of mating per fertilizing mating (P= 0.0001) with an average of 1 ± 0 in AI (Fertility rate of 80%), while it was of 1.45 ± 0.51 in natural breeding with a fertility rate of 61%. The weight of the rabbits at weaning was higher in AI than in natural mating (587.25 ± 338.19 g vs. 575.0 ± 375.44 g, respectively). The results relating to the number of total born, born alive, weaned rabbits, the farrowing-mating interval and the farrowing-fertilizing mating interval in natural mode were respectively of (7.4 ± 3.12), (4.55 ± 3.12), (4.55 ± 3.12), (12.65 ± 2.30), (19.15 ± 3.58); and in AI: (6.90 ± 4.12), (5.75 ± 4 .12), (5.75 ± 4.12), (11.65 ± 2.05), (18.99 ± 3.2) (P>0.05). Furthermore, and regarding the strains, data revealed that the females did not have any significant effect on the variables studied. On the contrary, the male had a very highly significant effect on the number of mating per fertilizing mating (p = 0.000), while it had no impact on the other parameters (P>0.05). To conclude, the control and the management of reproduction is the key to success in rabbit breeding, thus the combination of rearing condition and genetic effects is the main tool for making rabbit farming successful.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune
{"title":"Reproduction mode and Strain effects on Rabbit Breeding Performances","authors":"Khouloud Dekkiche, Hania Hannani, Achour Menanni, Nassima Ait Issad, D. Khelef, N. Mimoune","doi":"10.46419/vs.55.5.3","DOIUrl":"https://doi.org/10.46419/vs.55.5.3","url":null,"abstract":"This study was carried out to determine the impact of the mode of reproduction and the strain (male and female) of rabbits on some zootechnical and reproduction parameters. For that, monitoring was carried out in 2019 in the region of Setif (North Eastern Algeria) on a semi-intensive breeding of 25 crossbreed rabbits. The statistical analysis showed a very highly significant effect of the mode of reproduction on the number of mating per fertilizing mating (P= 0.0001) with an average of 1 ± 0 in AI (Fertility rate of 80%), while it was of 1.45 ± 0.51 in natural breeding with a fertility rate of 61%. The weight of the rabbits at weaning was higher in AI than in natural mating (587.25 ± 338.19 g vs. 575.0 ± 375.44 g, respectively). The results relating to the number of total born, born alive, weaned rabbits, the farrowing-mating interval and the farrowing-fertilizing mating interval in natural mode were respectively of (7.4 ± 3.12), (4.55 ± 3.12), (4.55 ± 3.12), (12.65 ± 2.30), (19.15 ± 3.58); and in AI: (6.90 ± 4.12), (5.75 ± 4 .12), (5.75 ± 4.12), (11.65 ± 2.05), (18.99 ± 3.2) (P>0.05). Furthermore, and regarding the strains, data revealed that the females did not have any significant effect on the variables studied. On the contrary, the male had a very highly significant effect on the number of mating per fertilizing mating (p = 0.000), while it had no impact on the other parameters (P>0.05). To conclude, the control and the management of reproduction is the key to success in rabbit breeding, thus the combination of rearing condition and genetic effects is the main tool for making rabbit farming successful.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho
{"title":"Razotkrivanje stanja: istraživanje prevalencije virusa mačje imunodeficijencije i virusa mačje leukemije među mačkama iz skloništa u Portugalu","authors":"Paulo Afonso, Luís Cardoso, Hélder Quintas, Ana Cláudia Coelho","doi":"10.46419/vs.55.5.1","DOIUrl":"https://doi.org/10.46419/vs.55.5.1","url":null,"abstract":"Feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) are important pathogens affecting feline hosts worldwide, compromising the health, well-being, quality of life, and lifespan of infected cats. This study explores the prevalence of these viral agents among shelter cats in Portugal, aiming to unravel their complexities and implications. The prevalence of FIV and FeLV was assessed by collecting blood samples (n = 326) from cats during medical routine check-ups at shelter admission and testing them with an immunochromatographic kit. The overall seroprevalence of FIV and FeLV was 15.3% (50/326, 95% confidence interval [CI]: 11.6–19.7). The proportion of cats positive to FIV, FeLV, and both FIV and FeLV was 9.8% (32/326, 95% CI: 6.8-13.6), 2.1% (7/326, 95% CI: 0.9-4.4) and 3.4% (11/326, 95% CI: 1.7-6.0), respectively. The prevalence of FIV and both FeLV and FIV appeared to be higher in the adult population. No statistical association was found between sex, breed, hair, or municipality, and FeLV, FIV and both FeLV and FIV prevalence. This is the first report on retroviral prevalence in shelter cats in Portugal, which provides important information regarding FIV and FeLV among stray cats in Portugal, highlighting the need for surveillance of those viruses, improved compliance with vaccination programmes, and vigilant management strategies to limit and control these viruses in the felinepopulation.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139841943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Šaljić, A. Hrković-Porobija, S. Šerić-Haračić, F. Tandir, Maja Varatanović, A. Ćutuk, B. Čengić
{"title":"The influence of sunflower oil in meals on the blood mineral profile of dairy cows","authors":"E. Šaljić, A. Hrković-Porobija, S. Šerić-Haračić, F. Tandir, Maja Varatanović, A. Ćutuk, B. Čengić","doi":"10.46419/vs.55.5.7","DOIUrl":"https://doi.org/10.46419/vs.55.5.7","url":null,"abstract":"Minerals are essential substances with an important function in livestock, and their disbalance negatively affects the health and production, especially of ruminants. The addition of fat to the rations of dairy cows impacts the overall and mineral metabolism, and thus on health and production. The aim of the research was to examine the correlative relationships between balanced production meals and the mineral status of lactating cows with different ration compositions with or without the presence of vegetable oil. The research was conducted on 30 dairy cows of the Holstein breed at the Butmir farm, Sarajevo, Bosnia and Herzegovina. Three groups of 10 animals were formed based on productivity (A, 13-15 kg of milk/day, B, 19-21 kg of milk/day and C, 25-27 kg of milk/day). Samples of feed, blood plasma and milk of animals were collected on three occasions a 3-week intervals. According to the feed analysis results, the rations were standardized for each group, given for three weeks without oil,and then for three weeks with the addition of sunflower oil in the amount of 2.5% of the ration dry matter. After determining the content of basic nutrients, dry matter and ash in the rations, the concentrations of Ca, Mg, Na and K were determined by atomic absorption spectrophotometry,while the colorimetric method was used to analyse P according to Woy and Eggertz-Finkener. Analysis of blood mineral parameters was performed spectrophotometrically. The content of fat, proteins, lactose, and non-fat dry matter in milk samples was determined by infrared spectrometry using an automatic analyser. Based on the results, we observed a similarity in changes of values and the statistical significance of the differences in plasma Ca, P and Mg between the study groups and at sampling intervals. Milk production was positively correlated with the concentration of Mg and Ca with addition of the sunflower oil to the meal, while no correlation coefficient was established for P in any of the samplings. Balanced meals with and without the addition of oil did not significantly affect the content of Ca, P or Mg in the blood plasma of the tested animals, nor were significant differences found between the groups. However, by determining the correlations between milk parameters and blood biochemical parameters, a significant positive correlation was established between the amount of milk and Ca and Mg levels with a diet supplemented by oil. Given that the addition of sunflower oil in the amount of 2.5% of dry matter of the balanced rations for cows had no negative effects on the mineral profile and the parameters of the tested animals, we conclude that the introduction of this oil could be useful both from the economic point and improving nutritional composition of milk as an animal food present in the daily human diet.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139779600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal
{"title":"Testing the potential antifungal activity of Origanum vulgare against Aspergillus fumigatus, Aspergillus Niger and Talaromyces marneffei isolated from pets","authors":"João Filipe Rama, Ana Cláudia Coelho, Fernanda Maria Leal","doi":"10.46419/vs.55.5.6","DOIUrl":"https://doi.org/10.46419/vs.55.5.6","url":null,"abstract":"Origanum vulgare (oregano) is an aromatic herb commonly used in the Mediterranean, widely known for its medicinal properties. In this experiment, we tested its antifungal properties against two species of Aspergillus (A. niger and A. fumigatus) and the emerging Talaromyces marneffei. We tested the plant’s antifungal activity in a range of concentrations (5, 10, 20 and 30 mg/mL) in which the plant was dissolved and mixed with growth medium (PDA) and then inoculated with the fungi. The percentage of inhibition was measured over 7 days with data being collected on the 3rd, 5th and 7th days. Fungal activity inhibition ranging from 30 to 100% was achieved against Aspergillus niger and Aspergillus fumigatus and from 50 to 100% against Talaromyces marneffei. This multi-disciplinary study brought the use of plants into the veterinary sciences by using samples isolated from pets, with the aim of researching potential alternatives to traditional antifungal treatments and the ultimate goal ofincreasing animal wellbeing.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Enrique Pardo-Pérez, Teodora Cavadía-Martínez, Luis Causil-Vargas
{"title":"Genetic characterisation of the domestic cat population (Felis catus) in Valledupar – Cesar using coat markers","authors":"Enrique Pardo-Pérez, Teodora Cavadía-Martínez, Luis Causil-Vargas","doi":"10.46419/vs.55.5.5","DOIUrl":"https://doi.org/10.46419/vs.55.5.5","url":null,"abstract":"The domestic cat (Felis catus) is a small carnivorous mammal belonging to the feline family. It is characterised by significant variability in terms of breeds, with females commonly smaller than males. Cats are one of the most popular pet species worldwide, though there is growing evidence that their large populations are having negative effects on the environment. Cats exhibit noticeable polymorphisms, particularly related to the colour, pattern, and texture of their coat, which can be easily recognised by visual inspection. This makes data collection a straightforward procedure. Cats are an ideal species for population studies because they are cosmopolitan animals and form a panmictic population. Genetic population analyses of cats are essential for understanding the history of their evolution and for developing phylogenetic hypotheses about allele relationships. However, despite the importance of these studies, global information availability is limited and, in some areas, completely absent. The aim of this study wasto determine the level of genetic diversity and structure in domestic cat populations using coat markers in Valledupar, Cesar. Random sampling was conducted between February and September 2022, and 365 adult animals were phenotypically identified in twelve neighbourhoods of the locality. The genes studied included Orange, Agouti, Dilution, Tabby, Long Hair, Siamese, Manx, Spotting White, and Dominant White. The Non-agoutimarker showed the highest frequency (0.740), while the Manx gene exhibited the lowest value. Most of the genetic diversity was found within populations and was limited between populations. Additionally, high gene flow, an excess of heterozygotes, and reduced genetic differentiation between populations were observed. The Spotting White locus showed a departure from Hardy-Weinberg equilibrium. Genetic distance between populations and the obtained dendrogram revealed low significant values, indicating that the populations as a whole are closely related.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139781624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}