{"title":"Seroprevalence of Aujeszky's disease in the domestic pig population in the Republic of Croatia from 2013 to 2021","authors":"Ž. Acinger-Rogić, Marina Pavlak, L. Jemeršić","doi":"10.46419/vs.54.5.9","DOIUrl":"https://doi.org/10.46419/vs.54.5.9","url":null,"abstract":"Aujeszky's disease is a viral disease of animals, primarily pigs, which are considered to be the natural hosts. It is endemic in many parts of the world, but there are areas and countries that have successfully eradicated this disease. Due to mandatory control measures and trade barriers, Aujeszky's disease causes significant economic losses. Although, it has been eradicated in most European Union Member States, there are still areas and countries where it persists. In the Republic of Croatia, the disease has not yet been eradicated from the domestic pig population, although surveillance and eradication has been carried out since 2013. This paper presents the results of serological testing of domestic pigs for Aujeszky's disease in the period from 2013 to 2021. The prevalence of Aujeszky's disease at the farm level by year in the study period ranged from 4.66% (CI 95%, 4.39-4.95) to 0.67% (CI 95%, 0.35-1.28). Since 2016, prevalence was at an average level of 1.26% (CI 95%, 0.67-1.95%). The highest prevalence 10.28% (CI 95%, 9.15-11.53) was found in Varaždin County, and the lowest 0.14 % (CI 95%, 0.07-0.31) in Krapina-Zagorje County.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43797290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Učinci radiofrekvencijskog elektromagnetskog zračenja na mušku plodnost","authors":"Ivona Žura Žaja, Ivan Folnožić, Jadranka Pejaković Hlede, Marko Samardžija, Anamaria Sluganović, Nikolino Žura, Silvijo Vince, Suzana Milinković-Tur, Marinko Vilić, Iva Butković, Paulina Martinec","doi":"10.46419/vs.54.5.6","DOIUrl":"https://doi.org/10.46419/vs.54.5.6","url":null,"abstract":"Elektromagnetsko se zračenje emitira iz prirodnog okruženja, ali i uporabom industrijskih i svakodnevnih uređaja za bežičnu komunikaciju, stoga su ljudski i životinjski organizmi stalno izloženi zračenju. Tijekom posljednjih godina, zbog brzog tehnološkog napretka, elektromagnetsko je zračenje iz umjetnih izvora premašilo je vrijednosti zračenja prirodnog podrijetla. Opća zabrinutost svih nas, zbog svevećeg broja uređaja (mobilni telefoni, prijenosna računala, Wi-Fi-ja i mikrovalnih pećnica), koji koriste radiofrekvencijsko elektromagnetsko zračenje (RF-EMZ) opravdana je zbog sve brojnijih dokaza o njihovoj štetnosti na žive organizme. Suvremeni uređaji moderne tehnologije emitiraju radiofrekvencijske elektromagnetske valove malih frekvencija koje ljudsko i životinjsko tijelo apsorbira što može imati potencijalne štetne učinke na: mozak, srce, endokrini sustav i reproduktivnu funkciju. Muški je reproduktivni sustav jedno od najosjetljivijih tkiva na RF-EMZ-e. Tako je primjerice, iz trenutno dostupnih studija provedenih in vitro i in vivo, jasno da RF-EMZ-e ima štetne učinke na spermatogenezu, odnosno kakvoću ejakulata ljudi i životinja – broj spermija u ejakulatu, preživljavanje, morfologiju i gibljivost spermija - utječu na stanični metabolizam i endokrini sustav i može prouzročiti genotoksičnost, genomsku nestabilnost i oksidativni stres, a to može prouzročiti neplodnost. Štetni učinci RFEMZ-a dijele se na: toplinske i netoplinske. Većinanegativnih bioloških učinaka pripisuje se netoplinskim učincima, a toplinski se učinci nastali RF-EMZ mobilnog telefona, smatraju manje štetnima. Zbog stvaranja prevelike količine reaktivnih kisikovih spojeva u muškom spolnom sustavu hipertermija skrotuma i povećani oksidativni stres mogu biti ključni mehanizmi putem kojih RF-EMZ-e utječe na plodnost muškaraca. Navedeni su i negativni učinci povezani s vremenom korištenja, ponajprije mobilnog telefona. Stoga je cilj ovoga preglednog rada opisati neke od učinaka RF-EMZ-a na muški spolni sustav.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44722709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marko Mitrovic, Ana U. Todorović, Zoran Vrbanac, Aleksandar Janevski, M. Lazarević Macanović
{"title":"Radiološki pregled zuba kunića","authors":"Marko Mitrovic, Ana U. Todorović, Zoran Vrbanac, Aleksandar Janevski, M. Lazarević Macanović","doi":"10.46419/vs.54.5.5","DOIUrl":"https://doi.org/10.46419/vs.54.5.5","url":null,"abstract":"Patološka su stanja zuba kunića u svakodnevnoj kliničkoj praksi vrlo česta i često rezultiraju poremećajem u funkciji organa digestivnog i respiratornog sustava, a mogu imati i letalan ishod. Da bi se omogućila pravovremena primena terapije i spriječila pojava komplikacija, posebnu pažnju treba usmjeriti na ranu dijagnostiku. Radiološka dijagnostika predstavlja jednostavan, brz i nezaobilazan postupak u procjeni stanja zubi. Za razliku od drugih vrsta sisavaca, radiološki pregled zuba kunića se odlikuje određenim osobitostima koje se, prije svega, očituju u tehnikama snimanja i interpretaciji rendgenskih snimaka koji su detaljno prikazani u ovom radu.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46473111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Selim Pašić, Nato Popara, Antea Klobučar, D. Cvitković, M. Vilić
{"title":"Entropy and its significance in transport of ions through the cell membrane","authors":"Selim Pašić, Nato Popara, Antea Klobučar, D. Cvitković, M. Vilić","doi":"10.46419/vs.54.5.8","DOIUrl":"https://doi.org/10.46419/vs.54.5.8","url":null,"abstract":"The aim of this paper is to present the concept of entropy in a simple way and to show its key importance in the transport processes of ions through the cell membrane using the latest discoveries in biophysics. Using a real-life example, we show how processes within a system lead to an increase in entropy. We also show how this entropy increase is directly related to the irreversibility of the process, and how it defines the arrow of time (direction of the flow of time). Using an abstract example, we clarify the meaning of the concept of disorder in a system, which is often used in defining entropy by connecting it with the number of microstates that realise a macroscopic state of a system. The importance of entropy in transport processes of ions through the cell membrane is considered. We show that passive transport processes through the cell membrane are the result of an entropy increase in the cell membrane-transported substance system. A model of active ion transport through the cell membrane following Rubi et al. (2017) is presented. The force that transports ions through the channel in the transport protein arises due to the entropy gradient formed along the transport channel, which is a consequence of the funnel shape of the channel. The entropic force is proportional to the ratio of the ion-available cross-sections of the exit and entrance surface of the channel. That means that only a very funnel-shaped channel can produce a sufficiently large force on the ions to overcome the concentration gradient of the substance. We analyse the final result for the force of entropy in the limits of a very wide and very narrow channel and find that the entropic force is proportional to the ratio of the areas of the exit to entrance surfaces of the channel, i.e., when the channel is very wide, while it becomes high as the width of the channel tends to the ion diameter, i.e., when the channel is very narrow. We explicitly explain how the presented model solves several fundamental questions about the active transport of substances: how is energy, a scalar quantity, converted into the directional motion of the ion (a vector quantity), how does energy drive ions considering that the point of release of energy is far from the point of binding of an ion in a transport protein and finally, how does energy, which is released in a very limited space, transport the ions over a very large spatial scale.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45884289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pasteurellosis: A significant bacterial disease in rabbit production","authors":"W. A. Abd El-Ghany","doi":"10.46419/vs.54.5.1","DOIUrl":"https://doi.org/10.46419/vs.54.5.1","url":null,"abstract":"Rabbits are susceptible to various respiratory affections. Pasteurellosis, caused by P. multocida, is regarded as one of the most important bacterial diseases of rabbits. The disease is characterised by chronic mucopurulent respiratory affection (snuffles) or more acute and subacute bronchopneumonia leading to high mortality and severe devastating losses in rabbit production. Moreover, pasteurellosis is associated with septicaemia, abscesses, otitis media, and nervous and reproductive disorders. P. multocida is widely distributed worldwide and mostly affects rabbits from 4 to 8 weeks of life. Infection with P. multocida usually occurs by indirect or direct contact, chiefly via aerosol. Moreover, the presence of other stressors can aggravate the severity of infection. The gold standard for the diagnosis of pasteurellosis is traditional isolation and identification methods. However, molecular techniques are used now for rapid detection of P. multocida and its virulence genes. Prevention is based on the application of hygienic methods, vaccination, and treatment using various antimicrobials. This review article gives an overview of pasteurellosis in rabbits regarding disease incidence, susceptibility and transmission, signs and lesions, laboratory diagnosis, and the prevention and control methods.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47212182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rima Sadeddine, S. Righi, Karim Daghri, K. Saidani, Ahmed Benakhala
{"title":"A comparative study of the arthropod fauna in stork, barn swallow and common pigeon nests","authors":"Rima Sadeddine, S. Righi, Karim Daghri, K. Saidani, Ahmed Benakhala","doi":"10.46419/vs.54.5.7","DOIUrl":"https://doi.org/10.46419/vs.54.5.7","url":null,"abstract":"An analysis was conducted on the arthropod fauna collected from stork, swallow, and pigeon nests during summer 2018 in three regions of north-eastern Algeria (Guelma, El-Tarf, and Annaba). A total of 40 nests was examined, of which 58% were infested with arthropods. Arthropods were less abundant and species rich in swallow nests than in pigeon or stork nests, where a greater dominance of mesostigmatic mites (78.71% and 72.41%, respectively) was found. Dermanyssus gallinae was the most abundant (33.05%) and most frequent (73.33%) mesostigmatic mite in pigeon nests, while uropodina mites were dominant (41.37%) in white stork nests with a frequency of 76.92% of the nests analysed. In swallow nests, the following arthropods were found: Psocodea, Hemiptera, Hymenoptera and Coleoptera belonging to the class Insecta and Isopoda belonging to the class Malacostraca. This is the first comparative survey of arthropods occupying the nests of these bird species in northern Algeria. It is anticipated that these data, that highlight the richness of the arthropod fauna in the nests of these bird species, will motivate further research aimed at characterising the arthropod community in different bird nests and determining the nature of the relationships between them.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41742041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Andreia Garcês, P. Brilhante-Simões, D. Duque, Filipe Sampaio, Augusto Silva, Ricardo Lopes, Delsy Salinas, Helder Fernandes
{"title":"First report of a cutaneous and shell mycosis in an Ouachita Map Turtle (Graptemys ouachitensis, Cagle, 1953) by Fusarium solani","authors":"Andreia Garcês, P. Brilhante-Simões, D. Duque, Filipe Sampaio, Augusto Silva, Ricardo Lopes, Delsy Salinas, Helder Fernandes","doi":"10.46419/vs.54.5.3","DOIUrl":"https://doi.org/10.46419/vs.54.5.3","url":null,"abstract":"An adult Ouachita Map Turtle (Graptemys ouachitensis) was presented for consultation with multifocal, erosive and ulcerative lesions, multiple fissures on the plastron and shell, and dermatitis on the members and head. Samples of shell scrapings were collected for routine microbial culturing and Fusarium solani was isolated from the samples. To the author’s knowledge, this is the first reported case of F. solani associated with cutaneous and shell mycosis in this turtle species.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49144371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Ječmenica, A. Humski, Louie Thomas Taylor, B. Šimpraga, F. Krstulović, Tajana Amšel Zelenika, L. Jurinović
{"title":"Diversity and prevalence of Salmonella spp. in gulls caught at a landfill, Zagreb, Croatia","authors":"B. Ječmenica, A. Humski, Louie Thomas Taylor, B. Šimpraga, F. Krstulović, Tajana Amšel Zelenika, L. Jurinović","doi":"10.46419/vs.54.5.2","DOIUrl":"https://doi.org/10.46419/vs.54.5.2","url":null,"abstract":"Gulls are a group of seabirds distributed worldwide that are an important reservoir of Salmonella spp. Salmonellosis is the second most commonly reported gastrointestinal infection in humans, and understanding the role wild birds have in spreading Salmonella can help to improve the health of humans and domestic animals. The mobility and migration capacity of gulls makes them an interesting group for research given their potential role in spreading pathogens. This paper presents the diversity and prevalence of Salmonella spp. in different gull species caught at a landfill in Zagreb in the winter months over a nine-year period from 2014-2022. In total, 1083 cloacal swabs were sampled from six gull species: Black-headed Gull (Larus ridibundus), Yellow-legged Gull (L. michahellis), Caspian Gull (L. cachinnans), Common Gull (L. canus), Lesser Black-back Gull (L. fuscus) and Herring Gull (L. argentatus). The prevalence of Salmonella was 5.82%, and 16 Salmonella serotypes were identified; S. Typhimurium had the highest prevalence (47.62%) followed by S. Enteritidis (12.69%) and S. Infantis (9.52%). To date, 82 Salmonella serotypes have been isolated in research on gulls in Europe, with S. Typhimurium as the most common, followed by S. Agona and S. Enteritidis. In this study, we found three serotypes not previously reported in gulls, S. Yalding, S. Reading and one with the antigenic formula O:17; H:z10; H:e,n,x,z15 (IIIb).","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49434562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Velma Rebić, M. Aljičević, V. Landeka, Džanela Mešinović, Amina Karić
{"title":"Pathogen presence in cottage cheese sold for public consumption at Sarajevo Canton markets","authors":"Velma Rebić, M. Aljičević, V. Landeka, Džanela Mešinović, Amina Karić","doi":"10.46419/vs.54.4.7","DOIUrl":"https://doi.org/10.46419/vs.54.4.7","url":null,"abstract":"Cottage cheese is the largest segment of the dairy market and is most often consumed as a fresh food. The microbiological quality of domestic cottage cheese can pose a problem for public health. Cottage cheese belongs to a group of foods having a potential public health risk. The aim of this study was to conduct microbiological research to determine the level of sanitary safety of cottage cheese types acquired from the most frequents markets of Sarajevo Canton (Bosnia and Herzegovina). Of the total (n=40) analysed cheese types, 22 samples (55%) proved to be meet sanitary requirements, while 18 samples (45%) did not meet microbiological quality, pursuant to the microbiological criteria for cheese stipulated by the National Regulation of Microbiological Criteria B&H and Guidelines for Microbiological Food Criteria B&H. The study included 24 samples of pasteurised and 16 samples of unpasteurised milk. However, five cheese samples (20.8%) from pasteurised milk, and 13 samples (81.3%) from unpasteurised milk were non-compliant. Microbiological analysis was conducted for compulsory and several recommended microorganisms: Salmonella spp., Listeria monocytogenes, coagulase positive staphylococci and other species (Staphylococcus aureus), Escherichia coli, and yeasts and moulds. Our findings confirm that the most common bacteria were S. aureus and E. coli. Also, yeasts and moulds were detected but were within the permissible concentrations. Salmonella spp. and L. monocytogenes were not detected in any of the examined samples.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70501194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Željka Anzulović Šanta, Anja Ognjenović, F. Božić, Boška Hrvačić, Ines Glojnarić, Snježana Čužić, Maja Antolić
{"title":"Retrospektivna procjena učinka nintedaniba u mišjem modelu plućne fibroze","authors":"Željka Anzulović Šanta, Anja Ognjenović, F. Božić, Boška Hrvačić, Ines Glojnarić, Snježana Čužić, Maja Antolić","doi":"10.46419/vs.54.4.4","DOIUrl":"https://doi.org/10.46419/vs.54.4.4","url":null,"abstract":"Pulmonary function tests (PFTs) routinely implemented in clinics are the first step in the diagnosis of idiopathic pulmonary fibrosis. Evaluation of PFTs in the mouse model of pulmonary fibrosis accompanied by histological readouts may improve the clinical predictability of new therapeutic candidates. Forced vital capacity (FVC) is considered the most predictive of restrictive pulmonary disorders. This study aimed to test the improvement of PFT in mice lung fibrosis induced by treatment with an approved substance nintedanib, considered the gold standard. The hypothesis that treatment in animal models will demonstrate similar effects as in humans in the most relevant clinical outcomes was tested. Two experimental designs were enrolled in this study, a preventive regimen, with treatment initiation from the day of the challenge; and a therapeutic regimen, starting on day 7 postchallenge when fibrotic changes are present in the lungs. Experiments were terminated at two different time points, at 14 and 21 days postchallenge. C57BL/6 mice were administered with bleomycin (BLM) intranasally and treated with nintedanib from day 0 to day 14 or from day 7 until day 21. Fourteen or 21 days after the BLM challenge, PFTs were assessed using the in vivo invasive lung function measurement system Buxco® Pulmonary Function Test (PFT) (DSI™, New Brighton, USA). Histological evaluation was performed as a modified Ashcroft score. The bleomycin challenge induced a significant decrease of FVC in both experiments. However, nintedanib treatment given in both regimens significantly improved lung functionality. These findings were confirmed with histological analysis of the Ashcroft scoring system, modified by Matsuse. In conclusion, a good correlation between functional test parameters and the clinical effect of nintedanib was shown in both experiments: the preventive regimen was sampled 14 days post-challenge and the therapeutic regimen 21 days post-challenge. Based on these findings, the implementation of PFTs could be a good platform to increase the translational value of the model and potential new treatments.","PeriodicalId":23596,"journal":{"name":"Veterinarska stanica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42002047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}