Veterinary World最新文献

{"title":"The protective effects of Saudi propolis against hepatic injury induced by gold nanoparticles in adult male albino rats.","authors":"Sarah A Althubyani","doi":"10.14202/vetworld.2025.252-262","DOIUrl":"10.14202/vetworld.2025.252-262","url":null,"abstract":"<p><strong>Background and aim: </strong>Gold nanoparticles (GNPs) are widely used in industrial and medical applications due to their unique properties but may induce oxidative stress and hepatotoxicity. Propolis, a bee-derived natural product with potent antioxidant and anti-inflammatory properties, shows promise as a hepatoprotective agent. This study evaluates the protective effects of Saudi propolis against GNP-induced hepatic damage by examining oxidative stress, lipid metabolism, and liver function. This study aimed to investigate the hepatoprotective effects of Saudi propolis against oxidative damage and lipid dysregulation induced by GNPs in male albino rats.</p><p><strong>Materials and methods: </strong>A total of 180 adult male rats were divided into six groups: (1) Control (saline), (2) Propolis (100 mg/kg), (3) GNPs (10 nm, 0.2 mg/kg/day), (4) GNPs (30 nm, 0.2 mg/kg/day), (5) GNPs (10 nm) + propolis, and (6) GNPs (30 nm) + propolis. Treatments were administered daily for 5, 10, or 15 days. Blood and liver samples were analyzed for oxidative stress markers, liver enzymes (aspartate transaminase, alanine transaminase, alkaline phosphatase, and glutamyl transpeptidase), lipid peroxidation (malondialdehyde [MDA]), antioxidant enzymes (superoxide dismutase [SOD] and glutathione peroxides [GPx]), and lipid profiles (cholesterol [CHO] and triglyceride [TG]).</p><p><strong>Results: </strong>Rats treated with GNPs showed elevated liver enzymes, lipid peroxidation, and oxidative stress, accompanied by increased CHO and TG levels. In contrast, co-administration of Saudi propolis significantly mitigated these effects, restoring MDA, SOD, and GPx levels close to control values. The hepatoprotective effects were more pronounced for 10 nm GNPs than 30 nm. After 15 days, TG levels returned to near-normal levels, while CHO levels improved but remained elevated.</p><p><strong>Conclusion: </strong>Saudi propolis exhibits significant protective effects against GNP-induced hepatic damage, primarily due to its antioxidant properties and ability to reduce oxidative stress and lipid peroxidation. The findings provide evidence for the therapeutic potential of propolis in managing nanoparticle-induced liver toxicity.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"252-262"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Veterinarians' perspectives on livestock diseases and antimicrobial use in Palestine.","authors":"Ibrahim M Alzuheir","doi":"10.14202/vetworld.2025.519-526","DOIUrl":"10.14202/vetworld.2025.519-526","url":null,"abstract":"<p><strong>Background and aim: </strong>The livestock sector is a crucial component of Palestine's agricultural economy, supporting food security and rural livelihoods. However, challenges such as infectious diseases, limited diagnostic resources, and antimicrobial misuse impact animal health and public safety. This study investigates veterinarians' perspectives on disease prevalence and antimicrobial use in Palestinian livestock, providing the first comprehensive analysis of antimicrobial resistance (AMR) in veterinary practice in the region.</p><p><strong>Materials and methods: </strong>A qualitative study was conducted using focus groups comprising 93 veterinarians from the West Bank, recruited through convenience and snowball sampling. A structured questionnaire collected data on disease prevalence and antimicrobial prescription patterns. Descriptive statistics and Chi-square tests were used to assess associations between demographic characteristics and veterinary practices.</p><p><strong>Results: </strong>Respiratory tract infections (RTIs) were the most frequently diagnosed disease (87.5%), followed by gastroenteritis (79.2%) and mastitis (75.0%). Veterinarians predominantly prescribed broad-spectrum antimicrobials, including penicillins (50.5%), tetracyclines (48.4%), and macrolides (46.2%). The use of antimicrobials classified as critically important for human medicine, such as quinolones (43.0%) and third-generation cephalosporins (46.2%), was notable. Some instances of banned antimicrobial use, such as chloramphenicol, were also reported.</p><p><strong>Conclusion: </strong>The findings highlight the reliance on empirical antimicrobial treatments and the widespread use of broad-spectrum and human-critical antimicrobials, raising concerns about AMR development. Improved antimicrobial stewardship, diagnostic capabilities, and regulatory frameworks are necessary to mitigate these risks. Policies promoting culture and sensitivity testing, along with targeted antimicrobial use, will enhance veterinary disease management and safeguard public health in Palestine.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"519-526"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic, hypolipidemic, and antioxidative properties of aqueous and ethanolic extracts of Sage (<i>Salvia officinalis</i> L.) against streptozotocin-induced diabetes and oxidative stress in Wistar albino male rats.","authors":"Yousef M Alharbi, Rashed A Aljalis, Hassan Barakat","doi":"10.14202/vetworld.2025.461-474","DOIUrl":"10.14202/vetworld.2025.461-474","url":null,"abstract":"<p><strong>Background and aim: </strong>Diabetes mellitus (DM) is a chronic disease characterized by insulin insufficiency and hyperglycemia, often leading to complications such as oxidative stress, dyslipidemia, and organs damage. Sage (<i>Salvia officinalis</i> L.), a medicinal plant with rich antioxidant and bioactive compounds, has shown promise in managing diabetes and related complications. This study investigates the antidiabetic, hypolipidemic, and antioxidative effects of aqueous (AE) and ethanolic (EE) extracts of <i>S. officinalis</i> leaves at doses of 400 and 800 mg/kg body weight in Wister albino male rats with streptozotocin (STZ)-induced type 2 diabetes and oxidative stress.</p><p><strong>Materials and methods: </strong>Wistar albino male rats (n = 49) were divided into seven groups: Normal control, diabetes-induced control (STZ), metformin-treated (50 mg/kg/day), and groups treated with AE (400 and 800 mg/kg/day) and EE (400 and 800 mg/kg/day). Parameters assessed included weight gain percentage, random blood glucose (RBG), fasting blood glucose (FBG), lipid profiles, liver and kidney function markers, oxidative stress biomarkers (glutathione [GSH], catalase [CAT], superoxide dismutase [SOD], malonaldehyde [MDA]), and histopathological examination of the pancreas.</p><p><strong>Results: </strong>AE and EE significantly reduced RBG and FBG and improved weight gain recovery. At 800 mg/kg, AE and EE effectively reduced triglycerides, total cholesterol, low-density lipoproteins cholesterol (LDL-C), and very LDL-C (VLDL-C) while increasing high-density lipoproteins cholesterol more than 400 mg/kg doses or metformin. Liver and kidney functions were restored with high-dose AE and EE showing superior efficacy. Antioxidant biomarkers (GSH, CAT, and SOD) were significantly enhanced, while MDA levels were reduced. Histopathological analysis confirmed restoration of islets of Langerhans and acinar cells to near-normal conditions in treated groups.</p><p><strong>Conclusion: </strong>The AE and EE of <i>S. officinalis</i> demonstrated potent antidiabetic, hypolipidemic, and antioxidative properties, offering significant potential as a natural therapeutic option for managing diabetes and oxidative stress-related complications.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"461-474"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of sea fish meat in the transmission of <i>Vibrio parahaemolyticus</i> to humans: An in-depth analysis of seasonal and species-specific variations.","authors":"Maged A Al-Garadi, Dhary Alewy Almashhadany, Rasha N Aziz, Dheyazan M Ali Al-Qabili, Ohoud S Alhumaidan, Hanouf Alnuwaysir, Al-Hammadi Mohammed Ali, Essam Sayed, A M Alabsi","doi":"10.14202/vetworld.2025.348-354","DOIUrl":"10.14202/vetworld.2025.348-354","url":null,"abstract":"<p><strong>Background and aim: </strong><i>Vibrio parahaemolyticus</i> is a marine bacterium commonly associated with foodborne illnesses due to the consumption of contaminated seafood. Understanding its prevalence in both fish meat and human infections is crucial for public health. This study aimed to estimate the occurrence of <i>V. parahaemolyticus</i> in human stool and fish meat samples while analyzing seasonal and species-specific variations in the Al-Hodeidah governorate.</p><p><strong>Materials and methods: </strong>A total of 225 samples were collected, including 75 human stool samples from patients with gastrointestinal symptoms and 150 fish meat samples from five fish species commonly consumed in the region. Standard microbiological methods were used for the isolation and identification of <i>V. parahaemolyticus</i>, including culture on Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) agar, biochemical tests, and growth analysis in varying NaCl concentrations. Data were statistically analyzed using SPSS version 12, applying the Chi-square test for group comparisons with a significance level of p ≤ 0.05.</p><p><strong>Results: </strong>The overall occurrence of <i>V. parahaemolyticus</i> was 7.1%. Human stool samples had a occurrence of 6.7%, while fish meat samples had a slightly higher occurrence of 7.3%. The highest monthly occurrence in human samples was recorded in July (15.0%), while the highest fish contamination was detected in September (12.0%). Among fish species, <i>Rastrelliger kanagurta</i> (Bagah) had the highest contamination rate (20.0%), followed by <i>Scomberomorus commerson</i> (Dairak) at 13.3%, whereas no <i>V. parahaemolyticus</i> isolates were found in <i>Dasyatis kuhlii</i> (Safon) and <i>Rachycentron canadum</i> (Sakalah).</p><p><strong>Conclusion: </strong>The findings confirm the presence of V. parahaemolyticus in both human and fish meat samples, highlighting seasonal variations and species-specific differences. The peak occurrence in fish during warm months suggests a potential link between higher temperatures and bacterial prevalence. Improved seafood handling, monitoring, and public health awareness are essential to mitigate the risk of foodborne infections. Further research is needed to explore genetic determinants of virulence and antimicrobial resistance in local isolates.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"348-354"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of ruminal ecology and blood profiles in Bali, Madura, and Ongole crossbred cattle of Indonesia.","authors":"Yenny Nur Anggraeny, Peni Wahyu Prihandini, Mozart Nuzul Aprilliza, Yeni Widiawati, Dicky Pamungkas, Mariyono Mariyono, Noor Hudhia Krishna, Risa Antari, Setiasih Setiasih, Bess Tiesnamurti, Muhammad Nasir Rofiq, Windu Negara, Eni Siti Rohaeni, Firsoni Firsoni, Wahidin Teguh Sasongko","doi":"10.14202/vetworld.2025.379-387","DOIUrl":"10.14202/vetworld.2025.379-387","url":null,"abstract":"<p><strong>Background and aim: </strong>Indonesian cattle breeds, primarily Bali, Madura, and Ongole crossbred (OC), are vital to local farming systems, yet little is known about their ruminal ecology and blood profiles. This study aimed to compare the rumen fluid characteristics and hematological parameters among these three indigenous cattle breeds.</p><p><strong>Materials and methods: </strong>Thirty heifers (10 per breed) were sourced from the Indonesian Beef Cattle Research Station. The animals, weighing 175-197 kg, were randomly allocated to individual pens. A diet of commercial concentrate and elephant grass (70:30 ratio) was provided at 3.5% of their body weight (dry matter basis). Blood samples were analyzed for glucose, blood urea nitrogen (BUN), and hematological indices (White blood cell, red blood cells [RBC], hemoglobin, hematocrit, mean corpuscular hemoglobin [MCH], and mean corpuscular volume [MCV]). Rumen fluid was assessed for pH, NH₃, volatile fatty acids (VFAs), and microbial diversity. Statistical analyses were performed using the Statistical Package for the Social Sciences with significance set at p < 0.05.</p><p><strong>Results: </strong>No significant differences were observed in blood glucose and BUN levels across breeds. Bali cattle exhibited the highest concentrations of total VFAs (139.66 mMol) and propionic acid (33.31 mMol), with a lower acetic-to-propionic acid ratio, reflecting efficient glucogenic traits. <i>Quinella</i>, a propionate-producing bacterium, dominated Bali cattle rumen microbiota. Conversely, OC cattle demonstrated the highest RBC count (9.27 x 10³/µL), while Bali cattle showed superior RBC size (MCV: 48.84 fl) and hemoglobin content (MCH: 16.60 pg).</p><p><strong>Conclusion: </strong>Bali cattle exhibited superior rumen fermentation efficiency and favorable hematological profiles, potentially supporting enhanced productive performance and reduced enteric methane emissions. These findings provide a foundation for breed-specific dietary management strategies to optimize local cattle productivity in tropical environments.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"379-387"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of multidrug-resistant <i>Proteus mirabilis</i> in poultry, livestock, fish, and the related environment: One Health heed.","authors":"Ayesha Sarwar, Bilal Aslam, Sara Mahmood, Saima Muzammil, Abu Baker Siddique, Fatima Sarwar, Mohsin Khurshid, Muhammad Hidayat Rasool, James Sasanya, Sulaiman F Aljasir","doi":"10.14202/vetworld.2025.446-454","DOIUrl":"10.14202/vetworld.2025.446-454","url":null,"abstract":"<p><strong>Background and aim: </strong>The emergence of multidrug-resistant (MDR) <i>Proteus mirabilis</i> in food-producing animals and their associated environments is a growing public health concern. The indiscriminate use of antimicrobials in animal husbandry exacerbates resistance development, posing significant threats to food safety and sustainability. This study investigates the distribution, antibiotic resistance patterns, and virulence-associated genes (VAGs) of <i>P. mirabilis</i> isolated from poultry, livestock, fish, and their environments in Pakistan under a One Health perspective.</p><p><strong>Materials and methods: </strong>A total of 225 samples were collected from poultry (n = 100), livestock (n = 75), and aquatic sources (n = 50) from March 2023 to September 2024. Standard microbiological methods were employed for the isolation and identification of <i>P. mirabilis</i>. Polymerase chain reaction (PCR)-based detection of antibiotic resistance genes and VAGs was performed using specific primers. Antibiotic susceptibility was assessed through the disk diffusion method following Clinical and Laboratory Standards Institute 2022 guidelines. Statistical analyses, including analysis of variance and correlation models, were applied to assess the relationships between variables.</p><p><strong>Results: </strong><i>P. mirabilis</i> was detected in 28.44% (64/225) of the total samples, with the highest occurrence observed in poultry (38%), followed by livestock (22.67%) and aquatic sources (18%). Resistance to ampicillin (100%), chloramphenicol (82%), cefepime (75%), and ciprofloxacin (75%) was widespread. PCR analysis revealed a high occurrence of extended-spectrum beta-lactamase-producing <i>P. mirabilis</i> carrying <i>bla</i> _CTX-M (49%), <i>bla</i> _OXA (54%), and <i>bla</i> _TEM (25.67%) genes. In addition, VAGs such as <i>zapA</i> (39.53%), <i>ucaA</i> (34.88%), and <i>hpmA</i> (32.55%) were frequently identified. The presence of MDR <i>P. mirabilis</i> in fish and related environments (18%) is alarming, highlighting potential zoonotic and foodborne transmission risks.</p><p><strong>Conclusion: </strong>The study underscores the widespread distribution of MDR <i>P. mirabilis</i> in animal-based food sources, raising significant concerns regarding food safety and antimicrobial resistance. The findings reinforce the need for stringent monitoring and regulatory policies to mitigate MDR bacterial dissemination across the food supply chain. Future research should employ metagenomic approaches for comprehensive surveillance and risk assessment.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"446-454"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of seaweed on blood plasma immunoglobulin concentration, mucosal immunity, small intestine histomorphology, cecal microbial population, and volatile fatty acid profile in broiler chickens.","authors":"Mohammad Naeem Azizi, Teck Chwen Loh, Hooi Ling Foo, Wan Ibrahim Izuddin","doi":"10.14202/vetworld.2025.508-518","DOIUrl":"10.14202/vetworld.2025.508-518","url":null,"abstract":"<p><strong>Background and aim: </strong>Seaweeds, particularly brown seaweed (BS) and green seaweed (GS), are rich in bioactive compounds that may enhance poultry health and productivity. This study evaluates the effects of dietary BS and GS on blood plasma immunoglobulin concentrations, mucosal immunity, small intestine histomorphology, cecal microbial populations, and volatile fatty acid (VFA) profiles in broiler chickens.</p><p><strong>Materials and methods: </strong>A total of 504 one-day-old male broilers were randomly assigned to 12 dietary treatments: A negative control (basal diet), a positive control (basal diet + 100 mg/kg Vitamin E), and diets supplemented with BS and GS at 0.25%, 0.50%, 0.75%, 1.00%, and 1.25%. The study followed a completely randomized design, with data analyzed using a one-way analysis of variance and Duncan's multiple range test (p < 0.05).</p><p><strong>Results: </strong>Broilers fed 0.75%, 1.00%, and 1.25% GS exhibited significantly higher (p < 0.05) blood plasma immunoglobulin A (IgA) and immunoglobulin G (IgG) concentrations. Dietary BS and GS inclusion upregulated messenger RNA expression of interleukin-6, interleukin-10, and interferon-gamma, indicating immunomodulatory effects. Jejunal villus height was significantly increased in birds fed 0.50%, 0.75%, and 1.25% BS during the starter period. Birds receiving 0.50% BS, 0.25% GS, and 0.50% GS exhibited higher cecal <i>Lactobacillus</i> counts, whereas 0.75% BS and GS significantly reduced <i>Escherichia coli</i> populations. Furthermore, higher total VFA and propionic acid concentrations were observed in birds supplemented with 1.00% and 1.25% GS, as well as 1.25% BS.</p><p><strong>Conclusion: </strong>The inclusion of GS (0.75%, 1.00%, and 1.25%) in broiler diets enhances immune response by increasing IgA and IgG levels. Both BS and GS positively modulate cytokine expression, intestinal morphology, and microbial balance, leading to improved gut health. The results suggest that BS and GS supplementation may serve as sustainable feed additives to enhance broiler performance while reducing reliance on synthetic supplements. Future studies should focus on identifying the bioactive compounds responsible for these effects and their broader implications for poultry production.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"508-518"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and antimicrobial-resistance gene profile of <i>Staphylococcus aureus</i> strains isolated from ovine mastitis in Jordan.","authors":"Mohammad H Gharaibeh, Tasneem A Mahafzah, Luay F Abu-Qatouseh, Malak Khanfar, Amir Abdulmawjood","doi":"10.14202/vetworld.2025.270-279","DOIUrl":"10.14202/vetworld.2025.270-279","url":null,"abstract":"<p><strong>Background and aim: </strong>Ovine mastitis, particularly subclinical mastitis caused by <i>Staphylococcus aureus</i>, poses significant economic and health challenges in livestock management. This study aimed to investigate the molecular characteristics and antimicrobial-resistance gene profiles of <i>S. aureus</i> isolated from subclinical mastitis in northern Jordan and assess the zoonotic potential of these isolates.</p><p><strong>Materials and methods: </strong>A total of 283 milk samples from ovines and 24 nasal swabs from animal handlers across three northern Jordanian governorates were analyzed. Bacterial isolates were identified phenotypically and genotypically, with antimicrobial susceptibility tested using disk diffusion and minimum inhibitory concentration assays. The presence of antimicrobial-resistance genes (ARGs) was analyzed through polymerase chain reaction, and genetic relatedness was determined using pulse-field gel electrophoresis (PFGE) and <i>spa</i> typing.</p><p><strong>Results: </strong><i>S. aureus</i> was detected in 16 (6%) ovine milk samples and four nasal swab samples from animal handlers. High resistance rates were observed for penicillin G, oxacillin, and cefoxitin (25% each). ARGs, including <i>mecA</i>, <i>blaZ</i>, <i>aph(3')-III</i>, and <i>ant(4')-Ia</i>, were detected in 25% of isolates. PFGE revealed a high genetic similarity among isolates, while <i>spa</i> typing identified three types, with <i>t1534</i> predominating (81.25%). Limited cross-transmission between ovines and handlers was noted.</p><p><strong>Conclusion: </strong>The study highlights <i>spa</i> type <i>t1534</i> as the dominant genotype in ovine mastitis in Jordan and underscores the low zoonotic transmission risk from ovines to animal handlers. The findings emphasize the importance of antimicrobial stewardship and enhanced mastitis management strategies.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 2","pages":"270-279"},"PeriodicalIF":1.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11963577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143781386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel surgical technique for cranial cruciate ligament repair in dogs using autologous lateral digital extensor muscle tendon graft combined with platelet-rich plasma: A preliminary experimental study.","authors":"Mousa H Daradka, Mays A Malkawi, Zuhair Banni Ismail, Hanan M Hammouri, Mahmoud A Abu-Abeeleh, Saba A Rihani","doi":"10.14202/vetworld.2025.210-219","DOIUrl":"10.14202/vetworld.2025.210-219","url":null,"abstract":"<p><strong>Background and aim: </strong>Cranial cruciate ligament (CrCL) injuries are a prevalent orthopedic issue in dogs, typically managed through surgical interventions such as tibial plateau leveling osteotomy and tibial tuberosity advancement. However, these techniques have limitations, including high costs and extended recovery periods. This study introduces an innovative CrCL repair method employing an autologous lateral digital extensor muscle tendon graft and evaluates the effects of platelet-rich plasma (PRP) on tissue healing.</p><p><strong>Materials and methods: </strong>Twenty-four healthy, male, local-breed dogs were divided into two groups. Group A underwent the surgical procedure without PRP, while Group B received intra-articular PRP during surgery. Outcomes were evaluated through clinical assessments of lameness, post-operative complications, and histological analysis over 10, 20, 30, and 40 days.</p><p><strong>Results: </strong>The PRP-treated group demonstrated statistically significant improvements in post-operative complication scores (p = 0.0025) and histological outcomes (p = 0.0002). However, graft maturation was unaffected by PRP treatment but improved over time (p = 0.0013). PRP-treated dogs exhibited faster recovery and enhanced tissue regeneration, with reduced inflammation and improved graft-bone attachment.</p><p><strong>Conclusion: </strong>This novel surgical approach demonstrates significant potential for improving outcomes in CrCL repair by combining autologous tendon grafting with PRP. The technique offers reduced complications and enhanced healing, providing a promising alternative to traditional methods. Further studies are recommended to validate its efficacy in clinical settings.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 1","pages":"210-219"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of circulating prolactin, lactation days, and seasonal variations on first artificial insemination pregnancy rates using the PG7G protocol in dairy cows.","authors":"Mufeed A Alnimer, Mohamed A Abedal-Majed, Mohmmad Al-Qaisi, Ahmad I Shamoun","doi":"10.14202/vetworld.2025.40-51","DOIUrl":"10.14202/vetworld.2025.40-51","url":null,"abstract":"<p><strong>Background and aim: </strong>Dairy farm profitability is linked to milk yield and reproductive efficiency. High prolactin levels during lactation can negatively impact fertility. Timed AI protocols like PG7G are used to improve pregnancy rates. This study investigates the effects of extending the voluntary waiting period (VWP) from 63 to 73 days after the PG7G protocol on reproductive parameters, including progesterone and prolactin levels, pregnancy rates, and pregnancy losses, in lactating Holstein cows during summer and winter seasons.</p><p><strong>Materials and methods: </strong>A total of 2100 lactating dairy cows were divided into groups and assigned to the PG7G protocol based on their parity. Two groups were formed based on the number of days in milk (DIM) after the first PGF_2α injection. The first group (PG7G-63) received a PGF_2α injection 30-day postpartum (pp) and followed a specific protocol. The second group, PG7G-73, followed the same protocol but received a PGF_2α injection 40-day pp. Pregnancy was confirmed, and blood samples were collected for analysis. Temperature and humidity were also recorded throughout the study.</p><p><strong>Results: </strong>Extension of the VWP to 73-day (PG7G-73 group) significantly improved pregnancy rates on day 47 pp compared with the 63-day VWP (PG7G-63 group). However, the PG7G-63 group exhibited lower PLs. Seasonality markedly influenced reproductive outcomes, with higher P/AI in the moderate season for primiparous cows and in the hot season for multiparous cows. Conversely, PL in both groups increased during the hot season.</p><p><strong>Conclusion: </strong>Extending the VWP from 63 to 73 days pp in lactating Holstein cows significantly improved pregnancy rates, while seasonality affected reproductive outcomes, with higher pregnancy rates in moderate temperatures and increased pregnancy loss during hot seasons.</p>","PeriodicalId":23587,"journal":{"name":"Veterinary World","volume":"18 1","pages":"40-51"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11873377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}