Tiezhu Chen , Tianyu Han , Yusong Miao , Liangchun Yan , Zhijun Liu , Han Dong , Tingting Cheng , Yiding Liu , Yu Yang , Shanshan Fei , Guoqiang Cheng , Guangliang Shi
{"title":"Cadmium exposure induced spleen inflammation by activating the MAPK/NF-κB/ NLRP3 signaling pathway and the intervention effect of astilbin","authors":"Tiezhu Chen , Tianyu Han , Yusong Miao , Liangchun Yan , Zhijun Liu , Han Dong , Tingting Cheng , Yiding Liu , Yu Yang , Shanshan Fei , Guoqiang Cheng , Guangliang Shi","doi":"10.1016/j.vetimm.2025.110889","DOIUrl":"10.1016/j.vetimm.2025.110889","url":null,"abstract":"<div><div>Cadmium (Cd) is an environmental pollutant with strong immunotoxicity effects. Despite this, the mechanisms by which Cd causes spleen damage in chickens are not well understood. Astilbin (ASB) is a dihydroflavone glycoside with anti-inflammatory and anti-oxidation properties. In the present study, a chicken spleen injury model induced by cadmium exposure (90d) was established to explore the specific mechanisms of Cd-induced spleen injury. If and how ASB ameliorates the damage was also explored. A total of 60 chickens were randomly divided into four groups: Con, Cd, ASB, and Cd+ASB groups. The pathological changes in the spleen were observed by H&E staining. Cd-induced oxidative stress, inflammation, and the involvement of the MAPK/NF-κB/NLRP3 in ameliorating spleen damage were also analyzed by Western blotting, qRT-PCR, and immunohistochemistry. Our results showed that Cd exposure for 90 days damaged the spleen, which mainly manifested as eosinophil infiltration, an increase in MDA content, a decrease in the CAT, GSH, SOD, and T-AOC, and activation of MAPK/NF-κB/NLRP3 signaling pathway. The overall outcome of these events was the induction of oxidative stress and inflammation in the spleen of the chickens. Interestingly, ASB treatment ameliorated Cd-induced damages. In conclusion, the present study revealed the specific mechanism of Cd-induced spleen damage using a chicken model. But ASB ameliorates Cd-induced cadmium poisoning.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"281 ","pages":"Article 110889"},"PeriodicalIF":1.4,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cynthia Pereira da Costa e Silva , Karina Médici Madureira , Vinicius Alvim Passos Baldacim , Juliana França dos Reis Costa , Bianca Paola Santarosa , Cristina de Oliveira Massoco Salles Gomes , Viviani Gomes
{"title":"Phagocytosis and reactive oxygen species (ROS) production by CH138+ granulocytes isolated from blood, colostrum, and milk of Holstein cows during transition period","authors":"Cynthia Pereira da Costa e Silva , Karina Médici Madureira , Vinicius Alvim Passos Baldacim , Juliana França dos Reis Costa , Bianca Paola Santarosa , Cristina de Oliveira Massoco Salles Gomes , Viviani Gomes","doi":"10.1016/j.vetimm.2025.110888","DOIUrl":"10.1016/j.vetimm.2025.110888","url":null,"abstract":"<div><div>This study investigated the functional activity of CH138 + granulocytes in the blood, colostrum, and transitional milk of Holstein cows throughout the transition period. Thirteen cows were assessed weekly, beginning three weeks before calving (W-3, W-2, and W-1), on the day of calving, and continuing to three weeks postpartum (W1, W2, and W3). Physical examination of the udder tissue, bacterial cultures, and blood immune function tests were performed. Microscopic Somatic Cell Count (MSCC), phagocytosis, and intracellular ROS production by CH138 + granulocytes in colostrum and transitional milk were measured weekly, from calving to W3. Disease incidence was recorded, with four cows developing metritis at W2. Mammary gland edema, affecting parenchymal tissue consistency, was the main physical finding. Non-Aureus Staphylococcus spp. dominated the bacterial isolates from the mammary secretions (67.5 %). MSCC was highest in colostrum and decreased postpartum. Bacterial culture rates peaked at calving; however, colostrogenesis limited the detection of clinical mastitis through specific examinations. Blood neutrophil function decreased at W-2 and W-1, likely increasing the risk of mammary infection and weakening the neutrophil response at calving. This immune suppression, which affects both the mammary gland and systemic immunity, was exacerbated by postpartum metritis. The findings showed heightened vulnerability to bacterial infection postpartum, which was linked to reduced cell viability and CH138 + granulocyte function. Neutrophil function in mammary secretions gradually improves post-calving, supporting immune recovery and declining infection rates. These findings provided valuable insights into the immune adaptations of CH138 + granulocytes in colostrum and transitional milk, enhancing understanding of mammary and systemic immune responses during the transition period.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"281 ","pages":"Article 110888"},"PeriodicalIF":1.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jayne E. Wiarda , Eraldo L. Zanella , Adrienne L. Shircliff , Eric D. Cassmann , Crystal L. Loving , Alexandra C. Buckley , Mitchell V. Palmer
{"title":"In situ staining with antibodies cross-reactive in pigs, cattle, and white-tailed deer facilitates understanding of biological tissue status and immunopathology","authors":"Jayne E. Wiarda , Eraldo L. Zanella , Adrienne L. Shircliff , Eric D. Cassmann , Crystal L. Loving , Alexandra C. Buckley , Mitchell V. Palmer","doi":"10.1016/j.vetimm.2024.110865","DOIUrl":"10.1016/j.vetimm.2024.110865","url":null,"abstract":"<div><div>Identifying cellular markers within archived formalin-fixed, paraffin-embedded (FFPE) tissues is critical for understanding tissue landscapes impacting animal health, but <em>in situ</em> detection methods are limited in veterinary species by a restricted toolbox of species-compatible immunoreagents. We identify antibodies with conserved <em>in situ</em> reactivity to IBA-1 (macrophages/dendritic cells), CD3ε (T cells), Pax5 (B cells), Ki-67 (cycling cells), and cytokeratin type I/II (epithelial cells) in FFPE tissues of pigs, cattle, and white-tailed deer. Multiplexed brightfield detection (IBA-1/CD3ε/Pax5) in lymph nodes of all three species demonstrated species-specific and species-conserved features of cellular architecture. Multiplexed fluorescent staining in pig lymph nodes for IBA-1/CD3ε/Pax5/Ki-67 allowed detection of colocalizing signals and identification of active germinal centers. Antibody compatibility with RNA <em>in situ</em> hybridization was confirmed for all antibodies in all species, allowing co-detection of RNA markers, which is a strategy highly useful in veterinary species where protein-reactive reagents are often lacking. Multiplexed protein and RNA staining was performed in tonsil tissue of a pig infected with Senecavirus A, enabling identification of virally-infected cell types via simultaneous detection of host cell type-specific proteins and virus-specific RNA. Findings have important applications for future <em>in situ</em> identification and comparative study of tissue landscapes and immunopathology in a diverse range of veterinary species.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110865"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cathelicidins in farm animals: Structural diversity, mechanisms of action, and therapeutic potential in the face of antimicrobial resistance","authors":"Ram Parsad, Sonika Ahlawat, Meena Bagiyal, Ritika Gera, Pooja Chhabra, Upasna Sharma, Reena Arora, Rekha Sharma","doi":"10.1016/j.vetimm.2024.110866","DOIUrl":"10.1016/j.vetimm.2024.110866","url":null,"abstract":"<div><div>Cathelicidins are a diverse family of antimicrobial peptides found across many vertebrate species, playing a pivotal role in the innate immune system. These peptides exhibit a variety of structural motifs, including α-helices, β-hairpins, and random coils, contributing to their broad-spectrum antimicrobial activity. The structural diversity of cathelicidins allows them to interact with a wide range of microbial targets, thereby enhancing their antimicrobial efficacy. Distinct species produce specific cathelicidins, each adapted to meet their unique immune requirements. Cathelicidins primarily function by disrupting microbial membranes, leading to cell lysis. Beyond their direct antimicrobial action, they possess immunomodulatory properties that bolster host defense mechanisms. These properties include promoting chemotaxis, enhancing phagocytosis, and inducing cytokine production, thereby modulating the host immune response. The therapeutic potential of cathelicidins is significant, especially in light of the growing challenge of antimicrobial resistance (AMR). As conventional antibiotics lose efficacy, cathelicidins emerge as promising alternatives due to their unique mechanisms of action and reduced likelihood of inducing resistance. Recent research underscores their potential in treating infections, inflammatory diseases, and even cancer. Advances in synthetic biology offer promising prospects for effective cathelicidin-based therapies in the future. This review summarizes the diversity, modes of action, and clinical prospects of cathelicidins specific to farm animals.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110866"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xia Huang , Xilong Kang , Shunzi Han , Chuang Meng , Hongqin Song , Xinan Jiao , Zhiming Pan
{"title":"Recombinant African swine fever virus p30–flagellin fusion protein promotes p30-specific humoral and cellular immune responses in mice","authors":"Xia Huang , Xilong Kang , Shunzi Han , Chuang Meng , Hongqin Song , Xinan Jiao , Zhiming Pan","doi":"10.1016/j.vetimm.2024.110864","DOIUrl":"10.1016/j.vetimm.2024.110864","url":null,"abstract":"<div><div>African swine fever (ASF) is a transmissible and deadly viral disease caused by the African swine fever virus (ASFV) that has considerably affected the global pig industry. Vaccination is considered a potentially effective method to control ASF. However, live attenuated vaccines can't protect against all circulating virus isolates. Subunit vaccines can induce both cellular and humoral immune responses, but often require the addition of adjuvants. Flagellin, a stimulator of Toll-like receptor 5 (TLR5), functions as a potent adjuvant by enhancing cellular and humoral immune responses. However, its high antigenicity may cause severe systemic inflammation. In this study, an <em>Escherichia coli</em> expression system was used to express ASFV p30 protein (p30) fused with <em>Salmonella</em> Typhimurium FliCΔD2D3 (without the D2 and D3 domains of FliC). The immunological effect of p30-FlicΔD2D3 protein in mice was evaluated. Results revealed that the ASFV p30 protein and the p30-FlicΔD2D3 fusion protein were effectively expressed by the <em>E. coli</em> expression system. <em>In vitro</em> activity analysis showed that the p30-FlicΔD2D3 fusion protein could be recognized by ASFV-positive serum, had good immunoreactivity, and remarkably promoted IL-8 secretion related to TLR5 activity in HEK293-mTLR5 cells. However, p30-FlicΔD2D3 induced significantly lower levels of inflammatory factor IL-8 than that induced by wild-type flagellin. Immunization with the p30-FlicΔD2D3 fusion protein considerably promoted cellular and humoral immune responses in mice. Therefore, the p30-FlicΔD2D3 protein retained good immune reactivity and TLR5 agonist efficacy. It also enhanced humoral and cellular immune responses in mice. This work offered valuable information that will be helpful to develop ASF subunit vaccines.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110864"},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laura I. Klepp , María Mercedes Bigi , Luciana Villafañe , Federico C. Blanco , Pauline Malinge l , Fabiana Bigi
{"title":"Production of functional bovine IL-22 in a mammalian episomal expression system","authors":"Laura I. Klepp , María Mercedes Bigi , Luciana Villafañe , Federico C. Blanco , Pauline Malinge l , Fabiana Bigi","doi":"10.1016/j.vetimm.2024.110863","DOIUrl":"10.1016/j.vetimm.2024.110863","url":null,"abstract":"<div><div>Interleukin 22 is a member of the interleukin-10 superfamily of cytokines. This protein has a dual role as an inflammatory and anti-inflammatory molecule dependent on the context. IL-22 is produced mainly by immune cells and seems to have non-hematopoietic cells as its target. In this work, we report the production of bovine IL-22 for the first time in a semi-stable expression system in mammalian cells. We showed that this recombinant IL-22 possesses biological activity in bovine macrophages infected with <em>Mycobacterium bovis</em> and is easy to produce in large quantities.</div><div>Given its role in the defence against infections, the IL-22 produced in this work has potential applications in scientific research as well as in immunotherapy to treat diseases in cattle.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110863"},"PeriodicalIF":1.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Samantha Randle , Lauri Wagter-Lesperance , Samantha Dixon , Umesh K. Shandilya , Bonnie A. Mallard , Byram Bridle , Niel A. Karrow
{"title":"Variation in ovine serum vitamin D concentrations from late summer to early spring and during immune challenge with bacterial endotoxin","authors":"Samantha Randle , Lauri Wagter-Lesperance , Samantha Dixon , Umesh K. Shandilya , Bonnie A. Mallard , Byram Bridle , Niel A. Karrow","doi":"10.1016/j.vetimm.2024.110856","DOIUrl":"10.1016/j.vetimm.2024.110856","url":null,"abstract":"<div><div>Vitamin D is most known for its role in the development and maintenance of the skeletal system. However, recent studies have found that vitamin D modulates the immune system, and at sufficient levels supports optimal immune function. Previous research has examined how vitamin D levels change seasonally and during immune stress challenges in both humans and cattle, but not sheep. Our study aimed to investigate the changes in the vitamin D status of sheep throughout the year in Southern Ontario as well as in response to a bacterial endotoxin challenge. Vitamin D was found to be significantly lower in spring when compared to summer and early winter levels. No significant differences were found in vitamin D levels among any measured time points during the immune challenge, although a trending increase was found 4 hr post-challenge. Overall, this study demonstrated how vitamin D levels in sheep change throughout the year, and slightly during endotoxin challenge. Given the importance of vitamin D in regulating optimal immune function, more investigation of these changes in vitamin D levels is warranted in terms of supporting optimal immune function.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110856"},"PeriodicalIF":1.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Pan , Rong-rong Wei , Ping Xu , Yun-ying Liu , Chen Li , Guo-wei Ding , Juan Fan , Yu-he Li , Jing-yi Yu , Peng Dai
{"title":"Progress in the application of Salmonella vaccines in poultry: A mini review","authors":"Jie Pan , Rong-rong Wei , Ping Xu , Yun-ying Liu , Chen Li , Guo-wei Ding , Juan Fan , Yu-he Li , Jing-yi Yu , Peng Dai","doi":"10.1016/j.vetimm.2024.110855","DOIUrl":"10.1016/j.vetimm.2024.110855","url":null,"abstract":"<div><div><em>Salmonella</em> is a critical group of zoonotic pathogens that are widely spread in poultry, causing avian salmonellosis. This disease usually leads to significant reductions in poultry performance, including reduced egg production in laying hens, decreased hatchability in chicks, and retarded growth in broilers. As a result, worldwide poultry industry suffers serious economic losses. Vaccination serves as an essential strategy for preventing <em>Salmonella</em> infection in poultry, effectively reducing susceptibility and alleviating disease symptoms, while also minimizing fecal shedding and environmental contamination. This subsequently diminishes public health risks. Various <em>Salmonella</em> vaccines can induce humoral and cellular immune responses to different extents. Therefore, a thorough understanding of the immune defense mechanisms, especially adaptive immune responses in poultry infected with <em>Salmonella</em>, is crucial for the development of <em>Salmonella</em> vaccines. This review summarizes the progress in the application of <em>Salmonella</em> vaccines in poultry, including adaptive immune responses induced by <em>Salmonella</em> and vaccines targeting the predominant circulating serotypes in poultry. It also provides an insight into the future of poultry-origin <em>Salmonella</em> vaccines.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110855"},"PeriodicalIF":1.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prognostic value of neutrophil to lymphocyte ratio and platelet indices in cats with feline panleukopenia","authors":"Kerim Emre Yanar","doi":"10.1016/j.vetimm.2024.110854","DOIUrl":"10.1016/j.vetimm.2024.110854","url":null,"abstract":"<div><div>The aim of this study was to ascertain the prognostic significance of the neutrophil to lymphocyte ratio (NLR) and platelet indices during hospital admission in cats with feline panleukopenia (FPV). A prospective observational study was conducted on 24 cats diagnosed with FPV. The results of the study demonstrated a significant decrease in NLR, platelet count (PLT), and platelet concentration (PCT) in the FPV survivor group compared to the control group. Furthermore, these parameters exhibited a notable decline in the FPV non-survivor group when compared to both the control group and the FPV survivor group. In contrast, mean platelet volume (MPV) and platelet distribution width (PDW) were significantly elevated in the FPV non-surviving cats compared to the control group. Moreover, receiver operating characteristic (ROC) curve analysis was employed to identify a prognostic cut-off value for predicting the prognosis of cats with FPV. The positive predictive values (PPVs) for survival were determined to be 90 %, 95 %, 85 %, 85 %, 85 %, and 75 %, respectively, using cut-off values of NLR (≤ 0.29), PLT (≤ 202.5 ×10³/µL), PCT (≤ 0.19 %), MPV (≥ 10.3 fL), and PDW (≥ 34.9 %). Based on the sensitivity, specificity and positive predictive values obtained from the ROC analysis, it was concluded that the NLR, MPV, and PLT are excellent biomarkers for determining prognosis in cats with FPV. Nevertheless, the PLT level is of greater significance, exhibiting a higher AUC.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110854"},"PeriodicalIF":1.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hyerim Do , Mingeun Sagong , Yoon kyoung Lee, Hyun-Kyu Cho, Youn-Jeong Lee, Seong-Hee Kim
{"title":"Efficacy of the H7N9 vaccine as a candidate for the Korean avian influenza antigen bank","authors":"Hyerim Do , Mingeun Sagong , Yoon kyoung Lee, Hyun-Kyu Cho, Youn-Jeong Lee, Seong-Hee Kim","doi":"10.1016/j.vetimm.2024.110851","DOIUrl":"10.1016/j.vetimm.2024.110851","url":null,"abstract":"<div><div>The avian influenza A virus (H7N9), first detected in China in 2013, is a zoonotic virus that remains persistent in bird populations despite a decline in human cases owing to control measures. Therefore, this study aimed to develop a vaccine as one preventive strategy in anticipation of the potential entry of H7N9 into Korea. Using the hemagglutinin and neuraminidase consensus sequences of H7N9 from 2018–2019, a recombinant H7N9 vaccine, rgAPQAH7N9, was developed, and its protective efficacy in specific pathogen-free chickens was evaluated. The rgAPQAH7N9 vaccine exhibited proliferation in eggs and demonstrated high immunogenicity, with a hemagglutination inhibition titer of 9.3 log<sub>2</sub>. Furthermore, the vaccine provided complete protection, as vaccinated chickens did not exhibit clinical signs or viral shedding. Moreover, when the rgAPQAH7N9 vaccine was boosted, the resulting immunity was long-lasting, with hemagglutination inhibition titers > 7 log2 persisting after 6 months. Therefore, the rgAPQAH7N9 vaccine virus may be considered a potential candidate for inclusion in the avian influenza antigen bank to ensure preparedness for emergency vaccination in poultry.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110851"},"PeriodicalIF":1.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}