Veterinary immunology and immunopathology最新文献

{"title":"Comparative analysis of host immune responses to Hydatid cyst in human and ovine hepatic cystic Echinococcosis","authors":"Bnar S. Hamad ,&nbsp;Bushra H. Shnawa ,&nbsp;Rafal A. Alrawi ,&nbsp;Mukhtar H. Ahmed","doi":"10.1016/j.vetimm.2024.110775","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110775","url":null,"abstract":"<div><h3>Background</h3><p>Hydatid disease is caused by the larval stages of the canine tapeworm <em>Echinococcus granulosus.</em> It is one of the most critical helminthic diseases, representing worldwide public health and socio-economic concern.</p></div><div><h3>Aim</h3><p>This study aimed to investigate the expression of apoptosis and immune response within hepatic tissues of humans and sheep infected with the Hydatid cyst.</p></div><div><h3>Methods</h3><p>Paraffin-embedded tissue was prepared from each tissue sample and used for histopathological examination by Haematoxylin- Eosin. Also, toluidine blue staining was used for mast cell detection, while an immunohistochemical study was performed to assess CD3 T lymphocytes, CD4 helper T lymphocytes, CD8 cytotoxic T lymphocytes, CD20 memory B lymphocytes, CD68 macrophage, and caspase-3 antibodies.</p></div><div><h3>Results</h3><p>The histological examination revealed significant changes, including the infiltration of inflammatory cells, predominantly lymphocytes with scattered giant cells, necrotic hepatic tissue, and fibrosis. Toluidine blue stain revealed a higher number of mast cells (5 cells/field) in humans compared to sheep (3.6 cells/field). The immunohistochemical analysis confirmed that the CD3 were the most predominant inflammatory cell in the hepatic tissue of humans (intensive 70%), and sheep (moderate 38.47%). Caspase-3 was observed in all samples in different grades and mostly in human liver tissue.</p></div><div><h3>Conclusion</h3><p>This data could aid in recognizing immunological markers for differentiating disease progression, as well as enhance the understanding of local immune responses to cystic Echinococcosis (CE). The findings could provide preliminary data for future studies on immune responses associated with Hydatid cysts.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"273 ","pages":"Article 110775"},"PeriodicalIF":1.8,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-evaluating the prevalence of anti-desmocollin-1 IgA autoantibodies in canine pemphigus foliaceus","authors":"Tyler J.M. Jordan ,&nbsp;Lisa B. Mamo ,&nbsp;Thierry Olivry ,&nbsp;Zhi Liu ,&nbsp;Petra Bizikova","doi":"10.1016/j.vetimm.2024.110773","DOIUrl":"10.1016/j.vetimm.2024.110773","url":null,"abstract":"<div><p>Pemphigus foliaceus (PF) is an autoimmune skin disease of dogs characterized by intraepidermal pustules containing neutrophils and dissociated keratinocytes that develop in association with circulating and tissue-bound IgG autoantibodies. A subset of IgG autoantibodies in canine PF target desmocollin-1 (DSC1), a component of intercellular adhesion complexes within the epidermis. Passive transfer of IgG autoantibodies from canine PF sera to mice was previously shown to induce skin disease in the absence of infiltrating neutrophils. In attempts to identify a mechanism responsible for neutrophil recruitment, past studies evaluated the prevalence of IgA autoantibodies in canine PF sera where they were found in &lt;20<!--> <!-->% of affected dogs. We re-evaluated the prevalence of anti-DSC1 IgA in canine PF due to concerns regarding the sensitivity of previously used methods. We hypothesized that anti-DSC1 IgA are present in most dogs with PF but have been under-detected due to competition with concurrent anti-DSC1 IgG for binding to their mutual antigenic target. Despite removing approximately 80<!--> <!-->% of IgG from patient sera using affinity chromatography, we did not detect an increase in anti-DSC1 IgA by performing indirect immunofluorescence on canine DSC1-transfected HEK293T cells. Taken together, our results do not support a role for pathogenic IgA in canine PF.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"273 ","pages":"Article 110773"},"PeriodicalIF":1.8,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of low milking frequency on the concentration of antimicrobial proteins in goat milk","authors":"Rika Harada,&nbsp;Takahiro Nii,&nbsp;Naoki Suzuki,&nbsp;Naoki Isobe","doi":"10.1016/j.vetimm.2024.110774","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110774","url":null,"abstract":"<div><p>This study examined the effects of low frequency milking on the concentrations of antimicrobial components in goat milk. Sixteen goats were divided into two groups of eight each: milking once every 2 d three times (for six days, three times group) or five times (for 10 days, five times group). On other days, milking was performed once daily. Milk was collected, and milk yield, somatic cell count (SCC), and the concentrations of some antimicrobial proteins such as lactoferrin (LF), S100A7, IgA, and sodium ions (Na⁺) in milk were measured. Milk yield significantly decreased in both the groups during the low-milking frequency period, followed by an increase above the low frequency milking period in both groups. In contrast, SCC and LF concentrations in milk increased in both groups during the low frequency milking period. The concentration of S100A7 in milk temporarily decreased after the low frequency milking period, followed by a significant increase. The S100A7 concentration during this period was higher in the five times group than in the three times group. These results indicated that low frequency milking induced a gradual decrease in milk yield and a concomitant increase in antimicrobial components, such as LF and S100A7, in milk. This increase in the antimicrobial components may be useful in preventing mastitis.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110774"},"PeriodicalIF":1.8,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoclonal antibody development advances immunological research in horses","authors":"Bettina Wagner","doi":"10.1016/j.vetimm.2024.110771","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110771","url":null,"abstract":"<div><p>Host immune analyses require specific reagents to identify cellular and soluble components of the immune system. These immune reagents are often species-specific. For horses, various immunological tools have been developed and tested by different initiatives during the past decades. This article summarizes the development of well characterized monoclonal antibodies (mAbs) for equine immune cells, immunoglobulin isotypes, cytokines, and chemokines.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110771"},"PeriodicalIF":1.8,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165242724000576/pdfft?md5=f2dd5935b1d3142f1be59423ac7cc4c2&pid=1-s2.0-S0165242724000576-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Necrosis plays a role in the concentration of mycobacterial antigens in granulomas from Mycobacterium bovis naturally infected cattle","authors":"Paola A. Ortega-Portilla ,&nbsp;Jacobo Carrisoza-Urbina ,&nbsp;Mario A. Bedolla-Alva ,&nbsp;Omar Cortéz-Hernández ,&nbsp;Mireya Juárez-Ramírez ,&nbsp;Guillermina Baay-Guzmán ,&nbsp;Sara Huerta-Yepez ,&nbsp;José A. Gutiérrez-Pabello","doi":"10.1016/j.vetimm.2024.110757","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110757","url":null,"abstract":"<div><p>The dynamics that develop between cells and molecules in the host against infection by <em>Mycobacterium bovis,</em> leads to the formation of granulomas mainly present in the lungs and regional lymph nodes in cattle. Cell death is one of the main features in granuloma organization, however, it has not been characterized in granulomatous lesions caused by <em>M. bovis</em>. In this study we aimed to identify the profiles of cell death in the granuloma stages and its relationship with the accumulation of bacteria. We identified necrosis, activated caspase-3, LC3B/p62 using immunohistochemistry and digital pathology analysis on 484 granulomatous lesions in mediastinal lymph nodes from 23 naturally infected cattle. Conclusions: greater amounts of mycobacterial antigens were identified in granulomas from calves compared with adult cattle. The highest percentage of necrosis and quantity of mycobacterial antigens were identified in granuloma stages (III/IV) from adults. The LC3B/p62 profile was heterogeneous in granulomas between adults and calves. Our data suggest that necrosis is associated with a higher amount of mycobacterial antigens in the late stages of granuloma and the development of autophagy appears to play an heterogeneous effector response against infection in adults and calves. These results represent one of the first approaches in the identification of cell death in the four stages of granulomas in bovine tuberculosis.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110757"},"PeriodicalIF":1.8,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165242724000436/pdfft?md5=6ea6dcfe024c645f3fb578ef8dfbb3f2&pid=1-s2.0-S0165242724000436-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and expression profiling of buffalo IFN-lambda family","authors":"Devika Gautam ,&nbsp;Anil Sindhu ,&nbsp;Ashutosh Vats ,&nbsp;Shiveeli Rajput ,&nbsp;Mayank Roshan ,&nbsp;Hanshika Pal ,&nbsp;Sachinandan De","doi":"10.1016/j.vetimm.2024.110770","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110770","url":null,"abstract":"<div><p>Interferon lambda (IFN-λ) is an important type III interferon triggered mainly by viral infection. IFN-λ binds to their heterodimeric receptors and signals through JAK-STAT pathways similar to type I IFN. In this study, we deduced the buffalo IFN-λ sequences through the polymerase chain reaction, and then studied IFN-λ’s expression patterns in different tissues, and post induction with poly I:C and live MRSA using RT-qPCR. The full-length sequences of buffalo <em>IFN-λ3</em>, IFN-λ receptors, and a transcript variant of IFN-λ4 were determined. IFN-λ1 is identified as a pseudogene. Virus response elements and a recombination hotspot factor was observed in the regulatory region of IFN-λ. The IFN-λ3 expressed highest in lungs and monocytes but IFN-λ4 did not. The expression of Interferon Lambda Receptor 1 was tissue specific, while Interleukin 10 Receptor subunit beta was ubiquitous. Following poly I:C induction, IFN-λ3 expression was primarily observed in epithelial cells as opposed to fibroblasts, displaying cell type-dependent expression. The cytosolic RNA sensors were expressed highest in endometrial epithelial cells, whereas the endosomal receptor was higher in fibroblasts. 2’,5’-oligoadenylate synthetase expressed higher in fibroblasts, myxoma resistance protein 1 and IFN-stimulated gene 56 in epithelial cells, displaying cell-specific antiviral response of the interferon stimulated genes (ISGs). The endometrial epithelial cells expressed IFN-λ3 after live <em>S. aureus</em> infection indicating its importance in bacterial infection. The induction of IFN-λ3 was <em>S. aureus</em> isolate specific at the same multiplicity of infection (MOI). This study elucidates the IFN-λ sequences, diverse expression patterns revealing tissue specificity, and specificity in response to poly I:C and bacterial stimuli, emphasising its crucial role in innate immune response modulation.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110770"},"PeriodicalIF":1.8,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective immunity induced through two calving seasons following administration of live epizootic bovine abortion agent (EBAA) vaccine","authors":"Myra T. Blanchard ,&nbsp;Mike B. Teglas ,&nbsp;Kassidy M. Collins ,&nbsp;Mark L. Anderson ,&nbsp;Bret R. McNabb ,&nbsp;Jeffrey L. Stott","doi":"10.1016/j.vetimm.2024.110772","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110772","url":null,"abstract":"<div><p>A live, infectious vaccine candidate for epizootic bovine abortion, designated EBAA Vaccine, USDA-APHIS Product code #1544.00, has been reported to be both safe and effective. Previous studies established that a single dose of EBAA vaccine administered to cows at potencies of either 2000 or 500 live <em>P. abortibovis</em>-infected murine spleen cells (<em>P.a.</em>-LIC) induced protective immunity for a minimum of 5 months. The current study employed 19 pregnant cows that were challenged with <em>P. abortibovis</em> in their 2nd trimester of gestation; 9 were vaccinated 17.2-months earlier as 1-year-olds with 2000 <em>P.a</em>.-LIC and 10 served as negative controls. Eighty-nine percent of the vaccinates gave birth to healthy calves as compared to 10% of challenge controls. Vaccine efficacy was significant when analyzed by prevented fractions (87.7%; 95% CI=0.4945–0.9781). Serologic data supports previous findings that pregnant cows with detectable <em>P. abortibovis</em> antibodies are immune to <em>P. abortibovis</em> challenge as demonstrated by the birth of healthy calves.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110772"},"PeriodicalIF":1.8,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140822265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An update on the development of a bottlenose dolphin, Tursiops truncatus, immune reagent toolkit","authors":"Randy.E. Sacco ,&nbsp;Eric D. Jensen ,&nbsp;Yvonne B. Sullivan ,&nbsp;Joanna LaBresh ,&nbsp;William C. Davis","doi":"10.1016/j.vetimm.2024.110769","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110769","url":null,"abstract":"<div><p>There are extensive immunological reagents available for laboratory rodents and humans. However, for veterinary species there is a need for expansion of immunological toolkits, with this especially evident for marine mammals, such as cetaceans. In addition to their use in a research setting, immune assays could be employed to monitor the health status of cetaceans and serve as an adjunct to available diagnostic tests. Such development of specific and sensitive immune assays will enhance the proper care and stewardship of wild and managed cetacean populations. Our goal is to provide immune reagents and immune assays for the research community, clinicians, and others involved in care of bottlenose dolphins. This review will provide an update on our development of a bottlenose dolphin immunological toolkit. The future availability and continued development of these reagents is critical for improving wild and managed bottlenose dolphin population health through enhanced assessment of their responses to alterations in the marine environment, including pathogens, and improve our ability to monitor their status following vaccination.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110769"},"PeriodicalIF":1.8,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165242724000552/pdfft?md5=c2df7884d36520fe642407fb04fd4fb4&pid=1-s2.0-S0165242724000552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140822264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resting and activated bovine neutrophils and eosinophils differ in their responses to adrenergic agonists","authors":"Angela Howell ,&nbsp;Natasa Arsic ,&nbsp;Philip Griebel","doi":"10.1016/j.vetimm.2024.110758","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110758","url":null,"abstract":"<div><p>Polymorphonuclear cells (PMN) provide a rapid response to infection and tissue damage and stress can modify these critical innate immune defences. The study of adrenergic receptor (AR) expression and function in bovine PMNs is limited but both neutrophils and eosinophils express numerous AR genes but differ significantly in their expression of individual AR genes. A flow cytometric technique was developed to differentiate between bovine neutrophils and eosinophils so both neutrophil and eosinophil responses to adrenergic agonists could be analysed. Neutrophils and eosinophils displayed significantly different changes in CD11b, L-selectin, and CD44 expression when activated by bovine serum opsonized zymosan and recombinant bovine interferon gamma. The responses of activated and resting neutrophils and eosinophils were then compared following stimulation with endogenous adrenergic agonists, epinephrine (E) norepinephrine (NE), and synthetic agonists targeting α1-, α2-, or β-ARs. Both resting and activated neutrophils and eosinophils displayed differences in iROS, CD44, and L-selectin expression following stimulation with E and NE. Resting neutrophils displayed pro-inflammatory responses to both E and NE, while resting eosinophils displayed a pro-inflammatory response to only NE. No single synthetic adrenergic agonist fully recapitulated responses observed with either E or NE and responses to adrenergic agonists were dose-dependent. In conclusion, bovine eosinophils and neutrophils responded to multiple adrenergic agonists by altering expression of proteins involved in immune surveillance and pro-inflammatory responses. Significant differences in neutrophil and eosinophil responses to adrenergic agonists are consistent with their differences in AR gene expression. This highlights the importance of analysing separately these two PMN subpopulations when investigating the effects of either endogenous or synthetic AR agonists.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110758"},"PeriodicalIF":1.8,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165242724000448/pdfft?md5=912f520d44e8fe04e77f8554edecfd50&pid=1-s2.0-S0165242724000448-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140647596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential antigenicity of individual Mycoplasma hyorhinis variable lipoproteins","authors":"Precy D. Magtoto ,&nbsp;Bailey L. Arruda ,&nbsp;Ronaldo L. Magtoto ,&nbsp;Juan Carlos Mora-Díaz ,&nbsp;Rina B. Opulencia ,&nbsp;David H. Baum ,&nbsp;Jeff J. Zimmerman ,&nbsp;Luis G. Giménez-Lirola","doi":"10.1016/j.vetimm.2024.110768","DOIUrl":"10.1016/j.vetimm.2024.110768","url":null,"abstract":"<div><p>The <em>Mycoplasma hyorhinis</em> (<em>Mhr</em>) variable lipoprotein (Vlp) family, comprising Vlps A, B, C, D, E, F, and G, are highly variable in expression, size, and cytoadhesion capabilities across <em>Mhr</em> strains. The ‘Vlp system’ plays a crucial role in cytoadhesion, immune evasion, and in eliciting a host immunologic response. This pilot study described the development of Vlp peptide-based ELISAs to evaluate the antigenic reactivity of individual Vlps against <em>Mhr</em> antisera collected throughout a longitudinal study focused on <em>Mhr</em> strain 38983, reproducing <em>Mhr</em>-associated disease under experimental conditions. Specifically, serum samples were collected at day post-inoculation 0, 7, 10, 14, 17, 21, 24, 28, 35, 42, 49, and 56 from <em>Mhr</em>- and mock (Friis medium)-inoculated cesarean-derived, colostrum-deprived pigs. Significant <em>Mhr</em>-specific IgG responses were detected at specific time points throughout the infection, with some variations for each Vlp. Overall, individual Vlp ELISAs showed consistently high accuracy rates, except for VlpD, which would likely be associated with its expression levels or the anti-Vlp humoral immune response specific to the <em>Mhr</em> strain used in this study. This study provides the basis and tools for a more refined understanding of these Vlp- and <em>Mhr</em> strain-specific variations, which is foundational in understanding the host immune response to <em>Mhr</em>.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"272 ","pages":"Article 110768"},"PeriodicalIF":1.8,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0165242724000540/pdfft?md5=d512d9054235515962262d4ab992959d&pid=1-s2.0-S0165242724000540-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}