Immunoprotective potential of egg yolk antibodies (IgYs) in controlling necrotic enteritis in broiler chickens

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-02-03 DOI:10.1016/j.vetimm.2025.110897

Zain Ul Abadeen , Muhammad Tariq Javed , Tariq Jamil , Syed Muhammad Faizan

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Abstract

The present study was designed to evaluate the immunoprotective role of specific egg yolk antibodies (IgYs). Eighty, day-old broiler chicks were purchased and divided into four groups (G₀, G₁, G₂ and G₃). G₀ served as control negative, while C. perfringens type A (1 ×10⁸ cfu/mL) was given via oral route from days 17–19 of the experiment in group G₁ (control positive). Groups G₂ and G₃ were passively immunized with 1 mL of anti-clostridial IgYs per bird via per os (days 21–24) and IM routes (days 22 and 24), respectively. The birds in group G₁ had higher feed conversion ratio (FCR) values and reduced relative weight of immune organs. The values of various immunological assays inlcuding hemagglutination inhibition titer values against Newcastle disease virus (NDV), titer values of total immunoglobulins and IgY after anti-sheep RBCs (anti-SRBCs) injection and phagocytic potential of circulating macrophages were lower in G₁ compared to G₀. In groups G₂ and G₃ (passively immunized), these pathological alterations were comparatively less severe or absent indicating the immuno-protective role of anti-clostridial IgYs against experimental infection. The results suggest that IgYs could be an effective alternative to antibiotics for controlling necrotic enteritis in poultry, with potential benefits in view of animal health and production costs.

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蛋黄抗体在控制肉鸡坏死性肠炎中的免疫保护作用

本研究旨在评价特异性蛋黄抗体（IgYs）的免疫保护作用。选取1日龄肉鸡80只，分为4组（G0、G1、G2和G3）。G1组为对照阴性，G1组从实验第17-19天开始口服A型产气荚膜原菌（1 ×108 cfu/mL），为对照阳性。G2组和G3组分别通过腹腔（第21 ~ 24天）和腹腔（第22、24天）被动免疫每只鸡1 mL抗梭菌IgYs。G1组雏鸟饲料系数（FCR）较高，免疫器官相对重量降低。各种免疫指标，包括抗新城疫病毒（NDV）血凝抑制滴度值、抗羊红细胞（抗羊红细胞）注射后总免疫球蛋白和IgY滴度值以及循环巨噬细胞吞噬电位，G1期均低于G0期。在被动免疫组G2和G3中，这些病理改变相对较轻或不存在，这表明抗梭菌IgYs对实验感染具有免疫保护作用。结果表明，IgYs可能是控制家禽坏死性肠炎的有效替代抗生素，从动物健康和生产成本的角度考虑，具有潜在的效益。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.