Veterinary immunology and immunopathology最新文献

Cathelicidins in farm animals: Structural diversity, mechanisms of action, and therapeutic potential in the face of antimicrobial resistance.

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-12-17 DOI: 10.1016/j.vetimm.2024.110866

Ram Parsad, Sonika Ahlawat, Meena Bagiyal, Ritika Gera, Pooja Chhabra, Upasna Sharma, Reena Arora, Rekha Sharma

{"title":"Cathelicidins in farm animals: Structural diversity, mechanisms of action, and therapeutic potential in the face of antimicrobial resistance.","authors":"Ram Parsad, Sonika Ahlawat, Meena Bagiyal, Ritika Gera, Pooja Chhabra, Upasna Sharma, Reena Arora, Rekha Sharma","doi":"10.1016/j.vetimm.2024.110866","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110866","url":null,"abstract":"<p><p>Cathelicidins are a diverse family of antimicrobial peptides found across many vertebrate species, playing a pivotal role in the innate immune system. These peptides exhibit a variety of structural motifs, including α-helices, β-hairpins, and random coils, contributing to their broad-spectrum antimicrobial activity. The structural diversity of cathelicidins allows them to interact with a wide range of microbial targets, thereby enhancing their antimicrobial efficacy. Distinct species produce specific cathelicidins, each adapted to meet their unique immune requirements. Cathelicidins primarily function by disrupting microbial membranes, leading to cell lysis. Beyond their direct antimicrobial action, they possess immunomodulatory properties that bolster host defense mechanisms. These properties include promoting chemotaxis, enhancing phagocytosis, and inducing cytokine production, thereby modulating the host immune response. The therapeutic potential of cathelicidins is significant, especially in light of the growing challenge of antimicrobial resistance (AMR). As conventional antibiotics lose efficacy, cathelicidins emerge as promising alternatives due to their unique mechanisms of action and reduced likelihood of inducing resistance. Recent research underscores their potential in treating infections, inflammatory diseases, and even cancer. Advances in synthetic biology offer promising prospects for effective cathelicidin-based therapies in the future. This review summarizes the diversity, modes of action, and clinical prospects of cathelicidins specific to farm animals.</p>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"110866"},"PeriodicalIF":1.4,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recombinant African swine fever virus p30-flagellin fusion protein promotes p30-specific humoral and cellular immune responses in mice. 重组非洲猪瘟病毒 p30-鞭毛蛋白融合蛋白可促进小鼠的 p30 特异性体液和细胞免疫反应。

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-12-10 DOI: 10.1016/j.vetimm.2024.110864

Xia Huang, Xilong Kang, Shunzi Han, Chuang Meng, Hongqin Song, Xinan Jiao, Zhiming Pan

{"title":"Recombinant African swine fever virus p30-flagellin fusion protein promotes p30-specific humoral and cellular immune responses in mice.","authors":"Xia Huang, Xilong Kang, Shunzi Han, Chuang Meng, Hongqin Song, Xinan Jiao, Zhiming Pan","doi":"10.1016/j.vetimm.2024.110864","DOIUrl":"https://doi.org/10.1016/j.vetimm.2024.110864","url":null,"abstract":"<p><p>African swine fever (ASF) is a transmissible and deadly viral disease caused by the African swine fever virus (ASFV) that has considerably affected the global pig industry. Vaccination is considered a potentially effective method to control ASF. However, live attenuated vaccines can't protect against all circulating virus isolates. Subunit vaccines can induce both cellular and humoral immune responses, but often require the addition of adjuvants. Flagellin, a stimulator of Toll-like receptor 5 (TLR5), functions as a potent adjuvant by enhancing cellular and humoral immune responses. However, its high antigenicity may cause severe systemic inflammation. In this study, an Escherichia coli expression system was used to express ASFV p30 protein (p30) fused with Salmonella Typhimurium FliCΔD2D3 (without the D2 and D3 domains of FliC). The immunological effect of p30-FlicΔD2D3 protein in mice was evaluated. Results revealed that the ASFV p30 protein and the p30-FlicΔD2D3 fusion protein were effectively expressed by the E. coli expression system. In vitro activity analysis showed that the p30-FlicΔD2D3 fusion protein could be recognized by ASFV-positive serum, had good immunoreactivity, and remarkably promoted IL-8 secretion related to TLR5 activity in HEK293-mTLR5 cells. However, p30-FlicΔD2D3 induced significantly lower levels of inflammatory factor IL-8 than that induced by wild-type flagellin. Immunization with the p30-FlicΔD2D3 fusion protein considerably promoted cellular and humoral immune responses in mice. Therefore, the p30-FlicΔD2D3 protein retained good immune reactivity and TLR5 agonist efficacy. It also enhanced humoral and cellular immune responses in mice. This work offered valuable information that will be helpful to develop ASF subunit vaccines.</p>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"110864"},"PeriodicalIF":1.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of functional bovine IL-22 in a mammalian episomal expression system

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-11-26 DOI: 10.1016/j.vetimm.2024.110863

Laura I. Klepp , María Mercedes Bigi , Luciana Villafañe , Federico C. Blanco , Pauline Malinge l , Fabiana Bigi

引用次数: 0

Variation in ovine serum vitamin D concentrations from late summer to early spring and during immune challenge with bacterial endotoxin 从夏末到初春以及在细菌内毒素免疫挑战期间绵羊血清维生素 D 浓度的变化。

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-11-22 DOI: 10.1016/j.vetimm.2024.110856

Samantha Randle , Lauri Wagter-Lesperance , Samantha Dixon , Umesh K. Shandilya , Bonnie A. Mallard , Byram Bridle , Niel A. Karrow

{"title":"Variation in ovine serum vitamin D concentrations from late summer to early spring and during immune challenge with bacterial endotoxin","authors":"Samantha Randle , Lauri Wagter-Lesperance , Samantha Dixon , Umesh K. Shandilya , Bonnie A. Mallard , Byram Bridle , Niel A. Karrow","doi":"10.1016/j.vetimm.2024.110856","DOIUrl":"10.1016/j.vetimm.2024.110856","url":null,"abstract":"<div><div>Vitamin D is most known for its role in the development and maintenance of the skeletal system. However, recent studies have found that vitamin D modulates the immune system, and at sufficient levels supports optimal immune function. Previous research has examined how vitamin D levels change seasonally and during immune stress challenges in both humans and cattle, but not sheep. Our study aimed to investigate the changes in the vitamin D status of sheep throughout the year in Southern Ontario as well as in response to a bacterial endotoxin challenge. Vitamin D was found to be significantly lower in spring when compared to summer and early winter levels. No significant differences were found in vitamin D levels among any measured time points during the immune challenge, although a trending increase was found 4 hr post-challenge. Overall, this study demonstrated how vitamin D levels in sheep change throughout the year, and slightly during endotoxin challenge. Given the importance of vitamin D in regulating optimal immune function, more investigation of these changes in vitamin D levels is warranted in terms of supporting optimal immune function.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"279 ","pages":"Article 110856"},"PeriodicalIF":1.4,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in the application of Salmonella vaccines in poultry: A mini review 沙门氏菌疫苗在家禽中的应用进展：小型综述。

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-11-13 DOI: 10.1016/j.vetimm.2024.110855

Jie Pan , Rong-rong Wei , Ping Xu , Yun-ying Liu , Chen Li , Guo-wei Ding , Juan Fan , Yu-he Li , Jing-yi Yu , Peng Dai

{"title":"Progress in the application of Salmonella vaccines in poultry: A mini review","authors":"Jie Pan , Rong-rong Wei , Ping Xu , Yun-ying Liu , Chen Li , Guo-wei Ding , Juan Fan , Yu-he Li , Jing-yi Yu , Peng Dai","doi":"10.1016/j.vetimm.2024.110855","DOIUrl":"10.1016/j.vetimm.2024.110855","url":null,"abstract":"<div><div><em>Salmonella</em> is a critical group of zoonotic pathogens that are widely spread in poultry, causing avian salmonellosis. This disease usually leads to significant reductions in poultry performance, including reduced egg production in laying hens, decreased hatchability in chicks, and retarded growth in broilers. As a result, worldwide poultry industry suffers serious economic losses. Vaccination serves as an essential strategy for preventing <em>Salmonella</em> infection in poultry, effectively reducing susceptibility and alleviating disease symptoms, while also minimizing fecal shedding and environmental contamination. This subsequently diminishes public health risks. Various <em>Salmonella</em> vaccines can induce humoral and cellular immune responses to different extents. Therefore, a thorough understanding of the immune defense mechanisms, especially adaptive immune responses in poultry infected with <em>Salmonella</em>, is crucial for the development of <em>Salmonella</em> vaccines. This review summarizes the progress in the application of <em>Salmonella</em> vaccines in poultry, including adaptive immune responses induced by <em>Salmonella</em> and vaccines targeting the predominant circulating serotypes in poultry. It also provides an insight into the future of poultry-origin <em>Salmonella</em> vaccines.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110855"},"PeriodicalIF":1.4,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic value of neutrophil to lymphocyte ratio and platelet indices in cats with feline panleukopenia 猫泛白细胞减少症患者中性粒细胞与淋巴细胞比率和血小板指数的预后价值。

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-11-07 DOI: 10.1016/j.vetimm.2024.110854

Kerim Emre Yanar

{"title":"Prognostic value of neutrophil to lymphocyte ratio and platelet indices in cats with feline panleukopenia","authors":"Kerim Emre Yanar","doi":"10.1016/j.vetimm.2024.110854","DOIUrl":"10.1016/j.vetimm.2024.110854","url":null,"abstract":"<div><div>The aim of this study was to ascertain the prognostic significance of the neutrophil to lymphocyte ratio (NLR) and platelet indices during hospital admission in cats with feline panleukopenia (FPV). A prospective observational study was conducted on 24 cats diagnosed with FPV. The results of the study demonstrated a significant decrease in NLR, platelet count (PLT), and platelet concentration (PCT) in the FPV survivor group compared to the control group. Furthermore, these parameters exhibited a notable decline in the FPV non-survivor group when compared to both the control group and the FPV survivor group. In contrast, mean platelet volume (MPV) and platelet distribution width (PDW) were significantly elevated in the FPV non-surviving cats compared to the control group. Moreover, receiver operating characteristic (ROC) curve analysis was employed to identify a prognostic cut-off value for predicting the prognosis of cats with FPV. The positive predictive values (PPVs) for survival were determined to be 90 %, 95 %, 85 %, 85 %, 85 %, and 75 %, respectively, using cut-off values of NLR (≤ 0.29), PLT (≤ 202.5 ×10³/µL), PCT (≤ 0.19 %), MPV (≥ 10.3 fL), and PDW (≥ 34.9 %). Based on the sensitivity, specificity and positive predictive values obtained from the ROC analysis, it was concluded that the NLR, MPV, and PLT are excellent biomarkers for determining prognosis in cats with FPV. Nevertheless, the PLT level is of greater significance, exhibiting a higher AUC.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110854"},"PeriodicalIF":1.4,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142628860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of the H7N9 vaccine as a candidate for the Korean avian influenza antigen bank 作为韩国禽流感抗原库候选疫苗的 H7N9 疫苗的功效

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-10-23 DOI: 10.1016/j.vetimm.2024.110851

Hyerim Do , Mingeun Sagong , Yoon kyoung Lee, Hyun-Kyu Cho, Youn-Jeong Lee, Seong-Hee Kim

{"title":"Efficacy of the H7N9 vaccine as a candidate for the Korean avian influenza antigen bank","authors":"Hyerim Do , Mingeun Sagong , Yoon kyoung Lee, Hyun-Kyu Cho, Youn-Jeong Lee, Seong-Hee Kim","doi":"10.1016/j.vetimm.2024.110851","DOIUrl":"10.1016/j.vetimm.2024.110851","url":null,"abstract":"<div><div>The avian influenza A virus (H7N9), first detected in China in 2013, is a zoonotic virus that remains persistent in bird populations despite a decline in human cases owing to control measures. Therefore, this study aimed to develop a vaccine as one preventive strategy in anticipation of the potential entry of H7N9 into Korea. Using the hemagglutinin and neuraminidase consensus sequences of H7N9 from 2018–2019, a recombinant H7N9 vaccine, rgAPQAH7N9, was developed, and its protective efficacy in specific pathogen-free chickens was evaluated. The rgAPQAH7N9 vaccine exhibited proliferation in eggs and demonstrated high immunogenicity, with a hemagglutination inhibition titer of 9.3 log<sub>2</sub>. Furthermore, the vaccine provided complete protection, as vaccinated chickens did not exhibit clinical signs or viral shedding. Moreover, when the rgAPQAH7N9 vaccine was boosted, the resulting immunity was long-lasting, with hemagglutination inhibition titers > 7 log2 persisting after 6 months. Therefore, the rgAPQAH7N9 vaccine virus may be considered a potential candidate for inclusion in the avian influenza antigen bank to ensure preparedness for emergency vaccination in poultry.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110851"},"PeriodicalIF":1.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of bovine IgG3-specific assays using a novel recombinant single-domain binding reagent 利用新型重组单域结合试剂开发牛 IgG3 特异性检测方法

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-10-23 DOI: 10.1016/j.vetimm.2024.110852

Alistair Noble , Elliot Moorhouse, Abigail L. Hay, Basudev Paudyal, William Mwangi , Danish Munir, Marie Bonnet-Di Placido, Elma Tchilian, John A. Hammond, Simon P. Graham

{"title":"Development of bovine IgG3-specific assays using a novel recombinant single-domain binding reagent","authors":"Alistair Noble , Elliot Moorhouse, Abigail L. Hay, Basudev Paudyal, William Mwangi , Danish Munir, Marie Bonnet-Di Placido, Elma Tchilian, John A. Hammond, Simon P. Graham","doi":"10.1016/j.vetimm.2024.110852","DOIUrl":"10.1016/j.vetimm.2024.110852","url":null,"abstract":"<div><div>Cattle express three subclasses of IgG antibody - IgG1, IgG2 and IgG3. Unlike IgG1 and IgG2, IgG3 was described relatively recently and the role of this subclass in immunity is unknown. Using recombinant bovine IgG1, IgG2 and IgG3 monoclonal antibodies (mAbs), we demonstrated that only one of the commercially available anti-bovine IgG mAbs tested was able to recognize IgG3, and no mAb exclusively bound IgG3. Here, we evaluated a small ankyrin repeat protein, called Ankyron™ AZS40101, that was generated to bind to bovine IgG3. Ankyron™ AZS40101 bound specifically to IgG3 with minimal reactivity to IgG1 and IgG2. Ankyron™ AZS40101 was shown to be useful in ELISA assays as either a capture or detection reagent. Utilisation of Ankyron™ AZS40101 alongside IgG1 and IgG2 specific mAbs to detect antigen-specific IgG subclasses in the serum of cattle sequentially vaccinated with heterologous foot-and-mouth disease virus capsid antigens revealed a low-level antigen-specific IgG3 response, in addition to IgG1 and IgG2 responses. Assessment of the total IgG1, IgG2 and IgG3 levels in healthy cattle plasma samples showed that IgG3 was measurable at a mean concentration of 0.19 mg/mL, although this was significantly lower than those of IgG1 (mean 3.28 mg/mL) and IgG2 (mean 3.41 mg/mL). Thus, Ankyron™ AZS40101 is a new reagent that provides utility for measurement of bovine IgG3 responses to infection and vaccination.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110852"},"PeriodicalIF":1.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142561110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solving technical issues in flow cytometry to characterize porcine CD8α/β expressing lymphocytes 解决流式细胞仪表征猪 CD8α/β 表达淋巴细胞的技术问题

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-10-22 DOI: 10.1016/j.vetimm.2024.110853

Florian Ringl , Maria Stadler , Katinka A. van Dongen , Mahsa Adib Razavi , Armin Saalmüller , Kerstin H. Mair

{"title":"Solving technical issues in flow cytometry to characterize porcine CD8α/β expressing lymphocytes","authors":"Florian Ringl , Maria Stadler , Katinka A. van Dongen , Mahsa Adib Razavi , Armin Saalmüller , Kerstin H. Mair","doi":"10.1016/j.vetimm.2024.110853","DOIUrl":"10.1016/j.vetimm.2024.110853","url":null,"abstract":"<div><div>The CD8 molecule is a cell surface receptor and well described as co-receptor on T cells, binding directly to the major histocompatibility complex class I on antigen presenting cells. CD8 antigens are comprised of two distinct polypeptide chains, the α and the β chain. In the pig, the CD8 receptor is expressed by several lymphocyte subsets, including Natural Killer cells, γδ T cells and antigen experienced CD4<sup>+</sup> αβ T cells. On these cell populations CD8 is expressed as αα homodimers. Porcine cytolytic T cells on the other hand exclusively express CD8 αβ heterodimers. Several monoclonal antibodies (mAbs) for either of the two chains are available and are frequently used in flow cytometry. We observed that distinct combinations of mAb clones for CD8α and CD8β chains can cause troubles in multi-color staining panels. Therefore, we aimed for an in-depth study of the usage of different CD8-specific mAb clones and optimizing co-staining strategies for flow cytometry. We tested mAb clones 11/295/33 and 76–2–11 for the detection of CD8α and mAb clones PPT23 and PG164A for the detection of CD8β. The results indicate that the CD8α clone 11/295/33 should not be used together with either of the two CD8β clones in the same incubation step, as co-staining led to a highly reduced ability of CD8β mAb binding and loss in signal in flow cytometry. This can lead to potential false results in detecting CD8αβ cytolytic T cells. In case of the CD8α mAb clone 76–2–11, no inhibition in binding of either CD8β mAb clones was observed, making it the preferred choice in multi-color staining panels. The obtained data will help in future panel designs for flow cytometry in the pig and therefore improving studies of porcine immune cells.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110853"},"PeriodicalIF":1.4,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of key immune genes in polarized porcine monocyte-derived macrophage subsets 极化猪单核细胞衍生巨噬细胞亚群中关键免疫基因的表达。

IF 1.4 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2024-10-12 DOI: 10.1016/j.vetimm.2024.110841

Giulia Franzoni , Floriana Fruscione , Filippo Dell’Anno , Lorena Mura , Chiara G. De Ciucis , Susanna Zinellu , Nicolò Columbano , Simon P. Graham , Silvia Dei Giudici , Elisabetta Razzuoli

{"title":"Expression of key immune genes in polarized porcine monocyte-derived macrophage subsets","authors":"Giulia Franzoni , Floriana Fruscione , Filippo Dell’Anno , Lorena Mura , Chiara G. De Ciucis , Susanna Zinellu , Nicolò Columbano , Simon P. Graham , Silvia Dei Giudici , Elisabetta Razzuoli","doi":"10.1016/j.vetimm.2024.110841","DOIUrl":"10.1016/j.vetimm.2024.110841","url":null,"abstract":"<div><div>Swine are considered one of the most relevant large animal biomedical models since they share many immunological similarities with humans. Despite that, macrophage polarization has not comprehensively investigated in pigs. In this study, porcine monocyte-derived macrophages (moMΦ) were untreated or stimulated with IFN-γ + LPS (classical activation), or by different M2 polarizing stimuli: IL-4, IL-10, TGF-β, or dexamethasone. Expression of key cytokine genes (<em>IL1B2, IL33, IL19, IL22, IL26, CCL17, CCL24, IFNA, IFNB)</em> in macrophage subsets were investigated over time<em>.</em> Expression of the genes encoding the two main enzymes of the arginine pathway (<em>ARG1</em>, <em>NOS2</em>), and molecules related to alternative macrophage polarization in human and mice (<em>MMP9, MRC1, FIZZ1, VEGFA)</em> were also assessed<em>.</em> Stimulation with IFN-γ + LPS triggered up-regulation of <em>IL1B2, IFNB, NOS2,</em> whereas IL-4 triggered upregulation of <em>CCL17, CCL24, CXCR2,</em> and <em>ARG1</em> expression. <em>IL19</em> and <em>IL22</em> expression was enhanced by stimulation with IFN-γ + LPS or TGF-β, but not IL-4, IL-10, or dexamethasone. Our data highlighted some peculiarities in swine, such as induced expression of <em>IL33</em> after stimulation with IFN-γ + LPS, and no up-regulation of <em>FIZZ1, VEGFA</em> or <em>MMP9</em> after exposure to any of the M2 polarizing stimuli. A better understanding of porcine macrophage polarization could benefit translational studies using this large animal model.</div></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":"278 ","pages":"Article 110841"},"PeriodicalIF":1.4,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0