Veterinary immunology and immunopathology最新文献_第10页

Enhancing bovine tuberculosis screening at Dhaka city in Bangladesh: Integrating gamma interferon blood test as ancillary testing with tuberculin skin test 孟加拉国达卡市加强牛结核病筛查：将γ-干扰素血液检测作为辅助检测与结核菌素皮肤检测相结合。

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-10-01 DOI: 10.1016/j.vetimm.2023.110659

Robiul Islam , Sk Shaheenur Islam , Tanzida Begum Rumi , Zakaria Mia , Zeaur Rahim

{"title":"Enhancing bovine tuberculosis screening at Dhaka city in Bangladesh: Integrating gamma interferon blood test as ancillary testing with tuberculin skin test","authors":"Robiul Islam , Sk Shaheenur Islam , Tanzida Begum Rumi , Zakaria Mia , Zeaur Rahim","doi":"10.1016/j.vetimm.2023.110659","DOIUrl":"10.1016/j.vetimm.2023.110659","url":null,"abstract":"<div><p>Tuberculin skin test (TST) is the standard method for screening of bovine tuberculosis (bTB). However, gamma interferon blood test has been introduced in the bTB control program as an ancillary testing with TST in many countries of the world. The objective of this study was to recommend this screening test as an ancillary testing with TST for field application in Bangladesh. In this study 577 cattle of different age, sex and breeds from twenty nine (29) cattle herds were examined to determine skin response against bTB through single intradermal comparative tuberculin test (SICTT) that comprised of positive (n = 81), inconclusive (n = 44) and negative (n = 452) animals. Of which 74 animals that included positive (n = 63), inconclusive (n = 8) and negative (n = 3) animals were taken under this study. Blood samples were collected in heparinized tube and stimulated overnight with bovine and avian purified protein derivatives (PPDs) for the secretion of gamma interferon, and measured via sandwich ELISA. Cohen’s kappa statistics was performed for the evaluation of agreement between the two tests. The agreement obtained between two tests was fair (Kappa agreement, K = 24.0%, 95% CI = 16.9−30.5%, P = 0.037). Of positive (n = 63), inconclusive (n = 8) and negative (n = 3) status of animals at SICTT, 82.54% (n = 52), 62.50% (n = 5), and 33.33% (n = 1) were found to be bTB positive respectively through this ancillary test. This test notably corroborates to TST result. A considerable number of inconclusive TB status animals were found to be positive through this gamma interferon assay. Therefore, this test could be used as an ancillary test with TST to maximize the proportion of bTB estimation in the infected cattle herd for early detection of zoonotic tuberculosis in Bangladesh before transmission at the animal-human interface.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41158458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significant increase in MIC-A and MIC-B and soluble MIC-A and MIC-B in canine lymphomas 犬淋巴瘤中MIC-A和MIC-B及可溶性MIC-A和MIC-B显著增加

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-10-01 DOI: 10.1016/j.vetimm.2023.110647

Maresa Lopez-Montaño , Laura Jimenez-Ortega , Teresa Rocio Cruz-Hernandez , Victor Gabriel Hernandez-Chavez , Laura Arcelia Montiel-Cervantes , Elba Reyes-Maldonado , Jorge Vela-Ojeda

{"title":"Significant increase in MIC-A and MIC-B and soluble MIC-A and MIC-B in canine lymphomas","authors":"Maresa Lopez-Montaño , Laura Jimenez-Ortega , Teresa Rocio Cruz-Hernandez , Victor Gabriel Hernandez-Chavez , Laura Arcelia Montiel-Cervantes , Elba Reyes-Maldonado , Jorge Vela-Ojeda","doi":"10.1016/j.vetimm.2023.110647","DOIUrl":"10.1016/j.vetimm.2023.110647","url":null,"abstract":"<div><p>Non-Hodkin's lymphoma (NHL) is the most frequent hematologic malignancy in humans and dogs. NKG2D is one of the most critical receptors on NK cells, recognizing their natural ligands on malignant cells such as A and B major histocompatibility complex-related proteins (MIC-A and MIC-B). Soluble molecules (sMIC-A and sMIC-B) can interfere with immune synapsis between NK cells and tumor cells, impeding NK cytotoxicity. The main objectives of this study were to analyze, in dogs with diffuse large B cell lymphoma, NK cell lymphoma, and reactive lymphadenopathies, the role of NK cells, their activating receptors NKG2D and NKp46, and their ligands MIC-A and MIC-B, as well as soluble molecules sMIC-A and sMIC-B. Thirty-six dogs with a possible diagnosis of NHL and eight healthy dogs were studied. NHL was diagnosed in 28 (78 %) dogs; in the other 8 (22 %), reactive lymphadenopathies were present. Most of the lymphomas corresponded to B cell NHL (82 %). The most predominant subtype was diffuse large B cell lymphoma (21, 71.5 %), followed by five cases (18 %) that were Non-B Non-T lymphomas (presumably NK cell lymphomas) and other B cell lymphomas (3, 10.5%). There were no cases of T cell NHL. MIC-A was positive in 7 of 27 (26 %) cases of NHL, and MIC-B in 20 of 27 (74 %) NHL. In non-malignant lymphadenopathies, three (37.5 %) dogs were positive for MIC-A, and five (62.5 %) expressed MIC-B. Dogs with lymphoma had higher numbers of NK cells than eight healthy dogs. In 15 dogs (12 cases with NHL and three cases with reactive adenopathies) and eight controls, there were no differences in the number of NK cells expressing NKP46 and NKG2D. NHL dogs had higher values of sMIC-A and sMIC-B. B-cell and NK cell lymphomas correspond to 86 % and 14 % of all canine lymphomas. MIC-A, MIC-B, and sMIC-A and sMIC-B were increased in canine lymphomas.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10167438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticulate chitosan-TNF-α-VLPs activate mast cells and enhance adaptive immunity induced by foot-and-mouth disease virus-like particles in mice 纳米壳聚糖TNF-α-VLP激活小鼠肥大细胞，增强口蹄疫病毒样颗粒诱导的适应性免疫。

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-10-01 DOI: 10.1016/j.vetimm.2023.110662

Ruoman Bai , Mingzhu Li , Zhanyun Tian , Yiming Hu , Manxin An , Wanzhe Yuan , Limin Li

{"title":"Nanoparticulate chitosan-TNF-α-VLPs activate mast cells and enhance adaptive immunity induced by foot-and-mouth disease virus-like particles in mice","authors":"Ruoman Bai , Mingzhu Li , Zhanyun Tian , Yiming Hu , Manxin An , Wanzhe Yuan , Limin Li","doi":"10.1016/j.vetimm.2023.110662","DOIUrl":"10.1016/j.vetimm.2023.110662","url":null,"abstract":"<div><p>Chitosan nanoparticulate vaccines have attracted considerable attention to potentiate immune responses. A chitosan-TNF-α-VLPs nanoparticle vaccine against foot-and-mouth disease virus (FMDV) prepared though inotropic gelation method and whether this nanoparticulate vaccine can activate mast cells and enhance immune responses induced by FMDV virus-like particles (VLPs) in mice was investigated. The nanoparticle was approximately spherical, and its size was approximately 200–300 nm. Following immunization via subcutaneous injection, the chitosan-TNF-α-VLPs nanoparticles could induce higher levels of FMDV-specific antibodies and stimulation index value than VLPs only (<em>P</em> < 0.01) and had similar levels to commercial vaccine group and VLPs+adjuvant group (<em>P</em> > 0.05). No significant differences were observed in the concentrations of IL-4, IFN-γ and IL-10 among the chitosan-TNF-α-VLPs group, VLPs+adjuvant group and commercial vaccine group (<em>P</em> > 0.05). Of note, the chitosan-TNF-α-VLPs nanoparticles can effectively activate mast cells in lymph nodes. These results indicated that the chitosan-TNF-α-VLPs nanoparticles can enhance both humoral and cell-mediated immunity, and both Th1 and Th2 responses, even activate mast cells, demonstrating that chitosan-TNF-α nanoparticles are potential as a vaccine adjuvant to enhance immune responses induced by FMDV-VLPs.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49682838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance of two commercial serological assays for bovine tuberculosis using plasma samples 两种商用牛结核病血浆血清学分析方法的性能

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI: 10.1016/j.vetimm.2023.110644

Charlotte Moens , Claude Saegerman , David Fretin , Sylvie Marché

{"title":"Performance of two commercial serological assays for bovine tuberculosis using plasma samples","authors":"Charlotte Moens , Claude Saegerman , David Fretin , Sylvie Marché","doi":"10.1016/j.vetimm.2023.110644","DOIUrl":"10.1016/j.vetimm.2023.110644","url":null,"abstract":"<div><p>In the bovine tuberculosis diagnosis, the use of plasma samples (already available for IFNɣ assays) in serological tests might facilitate the work in the field. Here, the performance of two commercial serological tests (ELISA IDEXX <em>M. bovis</em> Ab test and Enferplex Bovine TB antibody test) were evaluated using plasma samples from cattle in Belgium. Specificity values estimated from 567 plasma samples collected from bTB-free cattle were 98.4% when using the ELISA IDEXX <em>M. bovis</em> Ab test, and were 96.5% and 93.3% when using the high specificity and high sensitivity settings of the Enferplex Bovine TB antibody test, respectively. Sensitivity values were calculated relative to SICCT-positive (N = 117) and IFNɣ-positive (N = 132) animals originating from <em>M. bovis</em>-infected herds. Overall, the multiplexed Enferplex Bovine TB antibody test had better sensitivity (mean: 32.5% and 43.4% for the high specificity and sensitivity settings, respectively) compared to the ELISA IDEXX <em>M. bovis</em> Ab test (mean: 12%). Data obtained from plasma samples in the current study were compared to a previous study using both serological tests with sera. In conclusion, both serological tests showed comparable performance with both matrix; although overall specificity values with the Enferplex Bovine TB antibody test were lower when using plasma samples than sera.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10159068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses 马淋巴细胞免疫表型及渗出液和渗出液中MMP-9的浓度

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI: 10.1016/j.vetimm.2023.110645

Pavla Hamouzová , Olga Dobešová , Kristína Řeháková , Šárka Stehlíková , Petr Čížek , Zuzana Drábková , Petr Jahn , Jaroslav Doubek

{"title":"Lymphocyte immunophenotyping and concentration of MMP-9 in transudates and exudates in horses","authors":"Pavla Hamouzová , Olga Dobešová , Kristína Řeháková , Šárka Stehlíková , Petr Čížek , Zuzana Drábková , Petr Jahn , Jaroslav Doubek","doi":"10.1016/j.vetimm.2023.110645","DOIUrl":"10.1016/j.vetimm.2023.110645","url":null,"abstract":"<div><p>This study is the first to provide information on the lymphocyte subpopulations in peritoneal effusions in horses. Peritoneal transudates (n = 12), peritoneal exudates (n = 6) and a pleural exudate (n = 1) were analyzed. The total nucleated cell count (TNCC), total protein (TP) and matrix metalloproteinase-9 (MMP-9) concentration determined by ELISA were measured and routine cytological evaluation was performed. CD3, CD4, CD8 and CD21 positive cells were detected by flow cytometry. A higher percentage of neutrophils (P < 0.05) and higher MMP-9 (P < 0.01) levels were found in exudates. A higher percentage of macrophages (P < 0.05) and lymphocytes (P < 0.01) were found in transudates. CD4 + lymphocytes were the most common lymphocyte subpopulation in all samples. CD21 + lymphocytes were the least common in all samples. A large variability in the percentage of CD21 + lymphocytes was found in exudates. The percentage of CD21 + lymphocytes positively correlated with the level of total protein (r = 0.5704, P < 0.05). The correlation was even stronger in the group of exudates. The percentages of lymphocyte subpopulations did not correlate with the level of MMP-9 or with cytological findings. The level of MMP-9 positively correlated with the percentage of neutrophils (r = 0.4980, P < 0.05), the level of TP (r = 0.7855, P < 0.01) and TNCC (r = 0.6129, P < 0.01). A significantly higher level of MMP-9 was detected in euthanized horses than in horses that survived (P < 0.05). However, it was shown that the level of MMP-9 in the peritoneal fluid can change significantly in a short time. More studies on repeated abdominocentesis could contribute to elucidating the role of MMP-9 as a prognostic indicator.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10531818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of optimal storage time and temperature for the detection of red blood cell and platelet surface-associated immunoglobulin by flow cytometry in healthy horses 流式细胞术检测健康马红细胞和血小板表面相关免疫球蛋白的最佳保存时间和温度的确定

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI: 10.1016/j.vetimm.2023.110643

Miranda Frohlich , Kaori Knights , Nora L. Springer

{"title":"Determination of optimal storage time and temperature for the detection of red blood cell and platelet surface-associated immunoglobulin by flow cytometry in healthy horses","authors":"Miranda Frohlich , Kaori Knights , Nora L. Springer","doi":"10.1016/j.vetimm.2023.110643","DOIUrl":"10.1016/j.vetimm.2023.110643","url":null,"abstract":"<div><p>Differentiating immune-mediated causes from other causes of anemia and thrombocytopenia can be challenging. Flow cytometry can detect surface-associated immunoglobulin (sIg) on red blood cells (RBC) and platelets (PLT) in dogs and horses. Sample storage parameters for ideal assay performance has not been evaluated in horses. The study objective is to identify optimal storage time and temperature of equine whole blood for the detection of RBC-sIg and PLT-sIg via flow cytometry. Both assays were performed on samples at time 0, 4, 24, 48, and 72 h post collection. RBC-sIg samples were stored at 4 °C and PLT-sIg samples were stored at 4 °C and room temperature. RBC-sIg percentages were stable up to 72 h storage. Platelet surface-associated IgG percent positive platelets increased above baseline at all timepoints and percent positive platelets were inconsistent across timepoints for IgM and IgA. PLT-sIg testing should ideally be performed within 4 h of collection. In instances where this is not feasible, samples should be stored at 4 °C and analyzed no later than 24 h after collection. Whereas cutoff values for RBC-sIg remained similar across timepoints, results for PLT-sIg should be compared to time-specific cutoff or reference intervals established by the laboratory running the test.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10157201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bovine leukemia virus infection prolongs immunosuppression in dairy cows during the periparturient period by sustaining higher expression of immunological checkpoints in T cells 牛白血病病毒感染通过维持T细胞免疫检查点的高表达，延长奶牛围产期的免疫抑制

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI: 10.1016/j.vetimm.2023.110636

Alice Maria Melo do Nascimento , Carolina Menezes Suassuna de Souza , Ana Claudia Dumont Oliveira , Maiara Garcia Blagitz , Eduardo Milton Ramos Sanchez , Alice Maria Melville Paiva Della Libera , Ricardo de Miranda Henriques Leite , Artur Cezar de Carvalho Fernandes , Fernando Nogueira Souza

{"title":"The bovine leukemia virus infection prolongs immunosuppression in dairy cows during the periparturient period by sustaining higher expression of immunological checkpoints in T cells","authors":"Alice Maria Melo do Nascimento , Carolina Menezes Suassuna de Souza , Ana Claudia Dumont Oliveira , Maiara Garcia Blagitz , Eduardo Milton Ramos Sanchez , Alice Maria Melville Paiva Della Libera , Ricardo de Miranda Henriques Leite , Artur Cezar de Carvalho Fernandes , Fernando Nogueira Souza","doi":"10.1016/j.vetimm.2023.110636","DOIUrl":"10.1016/j.vetimm.2023.110636","url":null,"abstract":"<div><p>Bovine leukemia virus (BLV) is caused by a deltaretrovirus and has been associated with immunosuppression as well as comorbidities such as bovine mastitis, the costliest disease in the dairy sector. However, no previous study has explored at the synergistic immunosuppressive effect of the peripartum period with an immunosuppressive viral disease such as BLV. Thus, our study explored the effect of BLV infection in the periparturient period on the expression of PD-1 and CTLA-4 in blood T lymphocytes, and the impact of BLV infection on the rate of new intramammary infections during the early lactation. Here, we found that BLV-infected dairy cows always had a statistically significant higher expression of CTLA-4 and PD-1 in blood T cells. Furthermore, our findings indicated that BLV infection prolongs immunosuppression in dairy cows during the periparturient period by sustaining higher expression of immunological checkpoints in T cells. In addition, BLV-infected dairy cows have a higher rate of new intramammary infections during early lactation. Thus, our study provides new insights of the immunosuppressive effect of BLV on the most critical period of the cows’ life with marked detrimental effect on protective T-cell immunity and comorbidities, such as bovine mastitis.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10158588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cattle killer immunoglobulin-like receptor expression on leukocyte subsets suggests functional divergence compared to humans 牛杀伤免疫球蛋白样受体在白细胞亚群上的表达表明与人类相比功能上存在差异

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-09-01 DOI: 10.1016/j.vetimm.2023.110646

Abigail L. Hay , James Birch , Shirley Ellis , Daniel Burns , Salah Mansour , Salim I. Khakoo , John A. Hammond

引用次数: 1

Modulation of inflammatory and oxidative stress biomarkers due to dexamethasone exposure in chicken splenocytes 地塞米松对鸡脾细胞炎症和氧化应激生物标志物的调节作用

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-08-01 DOI: 10.1016/j.vetimm.2023.110632

Sonu Ambwani , Rigzin Dolma , Raunak Sharma , Amandip Kaur , Himani Singh , Anamitra Ruj , Tanuj Kumar Ambwani

{"title":"Modulation of inflammatory and oxidative stress biomarkers due to dexamethasone exposure in chicken splenocytes","authors":"Sonu Ambwani , Rigzin Dolma , Raunak Sharma , Amandip Kaur , Himani Singh , Anamitra Ruj , Tanuj Kumar Ambwani","doi":"10.1016/j.vetimm.2023.110632","DOIUrl":"10.1016/j.vetimm.2023.110632","url":null,"abstract":"<div><p>Dexamethasone (DEXA) is a potent corticosteroid, commonly used for treating inflammatory, hypersensitive and allergic conditions. It is administered to birds with tumours. Many studies were conducted on its immunosuppressive effects; however none of the similar study is available employing chicken splenocytes culture system. The present study was conducted to assess DEXA induced alterations in inflammatory and oxidative stress biomarkers in chicken splenocytes due to its <em>in vitro</em> exposure. The maximum non-cytotoxic dose (MNCD) was evaluated and was further used for conducting lymphocytes proliferation assay (LPA), antioxidant assays (lipid peroxidation, GSH, superoxide dismutase and nitric oxide assays) and assessment of mRNA levels of various genes (IL-1β, IL-6, IL-10, LITAF, iNOS, NF-κB1, Nrf-2, Caspase-3 and -9) through qPCR. The MNCD was determined to be 30 ng/ml in chicken splenocytes culture system. DEXA caused reduction in B and T lymphocytes proliferation indicating its immunosuppressive effects, however improved the antioxidant status of the exposed splenocytes. The expression levels of IL-1β, IL-6, iNOS, LITAF and NF-κB1 were significantly reduced while IL-10 was enhanced, which signify potent anti-inflammatory potential of DEXA. NF-κB is a major transcription factor that regulates genes responsible for both, innate and adaptive immune responses and elicits inflammation. The nuclear factor erythroid 2-related factor 2 (Nrf-2) level was found to be up-regulated. Nrf-2 plays important role in combating the oxidant stress and its increased expression could be the reason of improved antioxidant status of DEXA exposed cells. Present findings indicated that DEXA exhibited modulation in anti-inflammatory, immunomodulatory and antioxidant mediators in chicken splenocytes.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9977122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rapid detection of Mycobacterium bovis in bovine cytological smears and tissue sections by peptide nucleic acid fluorescence in-situ hybridization 肽核酸荧光原位杂交快速检测牛细胞学涂片和组织切片中的牛分枝杆菌

IF 1.8 3区农林科学

Veterinary immunology and immunopathology Pub Date : 2023-08-01 DOI: 10.1016/j.vetimm.2023.110635

Rabyia Javed , Deepti Narang , Kuldip Gupta , Sidartha Deshmukh , Mudit Chandra

{"title":"Rapid detection of Mycobacterium bovis in bovine cytological smears and tissue sections by peptide nucleic acid fluorescence in-situ hybridization","authors":"Rabyia Javed , Deepti Narang , Kuldip Gupta , Sidartha Deshmukh , Mudit Chandra","doi":"10.1016/j.vetimm.2023.110635","DOIUrl":"10.1016/j.vetimm.2023.110635","url":null,"abstract":"<div><h3>Background</h3><p>Bovine tuberculosis is the leading cause of death in cattle and other species worldwide. Quick and precise identification of mycobacteria is critical to control the occurrence of tuberculosis in cattle.</p></div><div><h3>Methods</h3><p>We developed a fluorescent peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) approach to detect <em>Mycobacterium bovis</em> and <em>Mycobacterium avium</em> in cytological smears and tissue sections of bovines suspected of having tuberculosis. PNA-FISH was conducted on smears of lung and lymph node tissues. Standard bovine mycobacterial cultures were used to standardize the probes using 50 % formamide for <em>M. bovis</em> and 30 % formamide for <em>M. avium</em>. <em>M. bovis</em> probe (MTBCcy3), which was standardized at hybridization conditions of (55 °C and 40 % formamide) concentrations, was positive in all cytological smears.</p></div><div><h3>Results</h3><p>Four out of twenty five samples tested positive in tissue sections observed as a bright red fluorescence with a cy3 filter (MTBC probe). No results were observed with (MAV<sub>TAMRA</sub>) probe for <em>M. avium</em> which was standardized at hybridization conditions of (55 °C and 30 % formamide). No fluorescence was observed in the control tissue sections. Additionally, the results were juxtaposed with those of other commonly used detection methods such as immunohistochemistry and Polymerase Chain Reaction (PCR) by targeting the <em>esxA</em> gene. None of the samples tested positive for <em>M. avium</em> infection.</p></div><div><h3>Conclusion</h3><p>PNA-FISH can be used to obtain cytological impression smears and tissue sections. When compared to PCR it consumes less time in the diagnosis of bovine tuberculosis in post mortem cases.</p></div>","PeriodicalId":23511,"journal":{"name":"Veterinary immunology and immunopathology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10339139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0