短讯：山羊乳腺炎与中性粒细胞胞外捕获物 (NET) 的形成。

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2024-06-19 DOI:10.1016/j.vetimm.2024.110793

Jéssica Lobo Albuquerque Caldeira , Daiene Gaione Costa , Richard Costa Polveiro , Maria Eduarda Gomes do Rêgo , Wagner Faria Barbosa , Leandro Licursi de Oliveira , Maria Aparecida Scatamburlo Moreira

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引用次数: 0

摘要

乳腺炎是一种影响奶牛产奶量和牛奶质量的乳腺炎症，通常与山羊的葡萄球菌有关。中性粒细胞是抗感染的关键，它们会迁移到牛奶中并采取各种防御策略，包括释放由 DNA、组蛋白和杀菌蛋白组成的中性粒细胞胞外捕获物（NETs）。本研究调查了山羊中性粒细胞在体外受到金黄色葡萄球菌和沃纳氏葡萄球菌（山羊乳腺炎的两种常见病原体）刺激时是否会释放 NETs。我们从健康成年山羊的血液中分离出 PMN。我们用以下物质刺激细胞，评估了山羊 NET 的形成：作为阳性对照的 12-肉豆蔻酸 13-乙酸磷脂（PMA）、用于抑制肌动蛋白聚合的细胞松、金黄色葡萄球菌和沃纳氏葡萄球菌。NET的形成是对化学刺激和细菌存在的反应，能有效地捕获病原体。金黄色葡萄球菌和华纳菌在形成 NET 方面的差异表明病原体具有特异性反应。这些发现表明，NET 的形成可能是防御山羊乳腺炎的一个重要补充机制。总之，本研究揭示了山羊的一种新型防御机制，表明了NET在乳腺炎中对金黄色葡萄球菌和华纳菌的作用。

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Short communication: Goat mastitis and the formation of neutrophil extracellular traps (NETs)

Mastitis, an inflammation of the mammary gland affecting milk production and quality in dairy herds, is often associated with Staphylococcus spp. in goats. Neutrophils are crucial in combating infections by migrating into milk and deploying various defense strategies, including the release of neutrophil extracellular traps (NETs) composed of DNA, histones, and bactericidal proteins. This study investigated whether NETs are released by goat neutrophils stimulated in vitro by Staphylococcus aureus and Staphylococcus warneri, two common pathogens of goat mastitis. PMNs were isolated from blood from healthy adult goats. We evaluated goat NET formation by stimulating cells with: phorbol 12-myristate 13-acetate (PMA) as a positive control, cytochalasin for inhibition of actin polymerization, S. aureus, and S. warneri. NET formation was observed in response to chemical stimulation and bacterial presence, effectively trapping pathogens. Variations in NET formation between S. aureus and S. warneri suggest pathogen-specific responses. These findings suggest that the formation of NETs may be an important complementary mechanism in the defense against mastitis in goats. In conclusion, this study unveils a novel defense mechanism in goats, indicating the role of NETs against S. aureus and S. warneri in mastitis.

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.