家禽口服免疫产生卵黄IgY及其鼻气管鸟thobacterium外膜特性研究

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2025-02-10 DOI:10.1016/j.vetimm.2025.110899

Muhammad Shahbaz Aslam , Summiya Khalid , Nadia Dar , Zaigham Abbas , Iram Gull , Zoha Khan , Sehreen Ashraf , Zahoor Qadir Samra

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引用次数: 0

摘要

由鼻气管鸟杆菌（ORT）引起的鸡呼吸道疾病“鸟杆菌病”严重影响了家禽养殖业。本研究通过在5 %羊血琼脂板上条带化，从鸡气管标本中分离到ORT，并进行形态学分析、生化试验和16S rRNA基因的物种特异性PCR扩增鉴定。将特征ORT培养，用福尔马林固定，与正常饲料混合，作为口服疫苗给蛋鸡接种10 d。口服疫苗接种后，用聚乙二醇沉淀法从鸡蛋中提取抗ort IgY抗体。IgY抗体进一步通过Native-PAGE、SDS-PAGE、ELISA和Western blot分析。卵细胞中IgY抗体的成功产生以及IgY抗体与ORT外膜作为抗原的相互作用表明，该口服疫苗可用于诱导鸡对ORT的体液免疫。此外，这些抗ort抗体可用于家禽业的诊断和治疗目的。这种口服疫苗接种技术可以转化为开发针对人类致病菌的蛋黄抗体。

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Production of IgY in egg yolk of Gallus gallus Domesticus by oral vaccination and its characterization with outer membrane of Ornithobacterium rhinotracheale

Chicken respiratory disease “Ornithobacteriosis” caused by Ornithobacterium rhinotracheale (ORT) badly affect the poultry industry. In this study, ORT was isolated from chicken tracheal samples by streaking on 5 % sheep blood agar plates and characterized by morphological analysis, biochemical tests and identification by species specific PCR amplification of 16S rRNA gene. Characterized ORT was cultured, fixed with formalin and mixed with the normal feed to be used as oral vaccine to egg laying hens for 10 days. After oral vaccination, anti-ORT IgY antibodies were extracted from eggs using PEG precipitation method. The IgY antibodies were further characterized by Native-PAGE, SDS-PAGE, ELISA and Western blot analysis. The successful production of IgY antibodies in eggs and interaction of IgY antibodies with outer membrane of ORT as antigen revealed that this oral vaccine can be used to induce humoral immunity in chickens against ORT. Furthermore, these developed anti-ORT antibodies could be used for the diagnostic and therapeutic purposes in the poultry industry. This oral vaccination technique can be translated to develop egg yolk antibodies against pathogenic bacteria in humans.

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.