Veterinary journal最新文献_第3页

Racing performance in 75 Thoroughbreds after arthroscopic removal of Osteochondritis dissecans from the lateral femoral trochlear ridge before first race start in Korea (2015–2017) 韩国第一场比赛开始前，75匹纯种马在关节镜下切除股骨外侧滑车脊的剥离性骨软骨炎后的比赛表现（2015-2017）

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-28 DOI: 10.1016/j.tvjl.2025.106367

Y. Sohn , S.J. An , E. Forbes , B.S. Kim , H.S. Jeon , S.-H. Ryu , I. Lee

{"title":"Racing performance in 75 Thoroughbreds after arthroscopic removal of Osteochondritis dissecans from the lateral femoral trochlear ridge before first race start in Korea (2015–2017)","authors":"Y. Sohn , S.J. An , E. Forbes , B.S. Kim , H.S. Jeon , S.-H. Ryu , I. Lee","doi":"10.1016/j.tvjl.2025.106367","DOIUrl":"10.1016/j.tvjl.2025.106367","url":null,"abstract":"<div><div>The lateral trochlear ridge of the stifle joint is highly susceptible to developing Osteochondrosis (OC)/Osteochondritis dissecans (OCD) lesions. This retrospective study evaluated the medical and racing outcomes of 75 Thoroughbreds who underwent arthroscopic OCD removal at the Jeju Stud Farm Equine Hospital from 2015 to 2017. The analysis included medical records, radiographic assessments, and race performance data, comparing 75 horses that underwent surgery with 257 control horses selected as siblings from the same dam. The surgical group had comparable racehorse registration rates (89.3 %) and first race start rates (72.0 %) compared to those of the control group (84.8 % and 74.7 %, respectively). However, horses that underwent surgery had their first race start later (<em>p</em> = 0.000864, <em>p</em> < 0.0001) and participated in fewer total races (<em>p</em> = 0.01708, <em>p</em> < 0.05) than the control group. Despite these differences, there were no significant variations in sales prices, career earnings, race points, or retirement age. Furthermore, neither the size of the OCD lesion nor the timing of the surgery significantly influenced overall racing performance metrics, except for a reduction in career duration for horses operated on at an older age. Sex-based analyses revealed that male horses consistently outperformed females in career duration and earnings. Subgroup analyses revealed significantly poorer outcomes in females treated before 12 months of age and those with large lesions (≥ 40 mm), including fewer starts and earlier retirement. In summary, arthroscopic surgery for stifle OCD in Thoroughbreds before their first race start did not negatively affect overall performance. While lesion size and surgery timing had minimal impact, this study suggests that sex-related factors may more strongly influence post-surgical outcomes.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106367"},"PeriodicalIF":2.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of altered distances between A-frame and the preceding jump on front limb dynamics in agility dogs a架与前跳距离的改变对敏捷犬前肢动力学的影响

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-24 DOI: 10.1016/j.tvjl.2025.106363

Claire Bidwell , Scott Blake , David Marlin , Roberta Blake

{"title":"The effects of altered distances between A-frame and the preceding jump on front limb dynamics in agility dogs","authors":"Claire Bidwell , Scott Blake , David Marlin , Roberta Blake","doi":"10.1016/j.tvjl.2025.106363","DOIUrl":"10.1016/j.tvjl.2025.106363","url":null,"abstract":"<div><div>A high proportion of agility injuries associated with an obstacle are due to the A-frame, however, there is limited research into the kinetics and kinematics of dogs traversing this type of equipment. The aim of this research was to study the kinematics and kinetics of agility dogs negotiating an A-frame when the preceding obstacle (in this case a jump) was placed at 10 m, 7.5 m and 5 m ahead of the A-frame. Six competition standard agility dogs were recorded negotiating an A-frame after completing a jump with each dog attempting each distance three times. Inertial measuring units attached to each dog gathered maximum velocity, acceleration and deceleration between jump landing and the A-frame. Video analysis and pressure sensors gathered carpal hyperextension and peak vertical forces for both forelimbs at the dogs’ contact with the A-frame. The study found no difference in either carpal extension or PVF data between the different distances. However, maximum approach velocity decreased (p < 0.05) with decreasing distance: 10 m (7.30 ± 0.40 m/s), 7 m (6.61 ± 0.34 m/s), and 5 m (5.74 ± 0.62 m/s). Acceleration was also decreased at the 5 m distance compared with 10 m distance (p < 0.05). A notable finding was the −1.57 m/s<sup>2</sup> decrease in deceleration found between the 10 m (-5.92 m/s<sup>2</sup>) and 5 m (-4.35 m/s<sup>2</sup>) distances (p < 0.05), with the 10 m distance having 36 % more deceleration than 5 m. As forelimbs have a role in deceleration, an increased distance between obstacles could be one of the factors involved in forelimbs injuries in agility dogs. In our study, positioning the preceding obstacle 5 m from the A-frame moderated speed, acceleration, and deceleration, and could potentially help to reduce reported injury rates, but additional studies are recommended to allow evidence-based guidelines.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106363"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Population pharmacokinetics and clinical evaluation of intravenous acetaminophen and its metabolites in Andalusian horses 安达卢西亚马静脉注射对乙酰氨基酚及其代谢物的人群药代动力学和临床评价

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-24 DOI: 10.1016/j.tvjl.2025.106357

M.M. Granados , F. Medina-Bautista , R. Navarrete-Calvo , D. Argüelles , J.M. Domínguez-Pérez , F. Priego-Capote , P. Muñoz-Rascón , J.M. Serrano-Rodríguez

{"title":"Population pharmacokinetics and clinical evaluation of intravenous acetaminophen and its metabolites in Andalusian horses","authors":"M.M. Granados , F. Medina-Bautista , R. Navarrete-Calvo , D. Argüelles , J.M. Domínguez-Pérez , F. Priego-Capote , P. Muñoz-Rascón , J.M. Serrano-Rodríguez","doi":"10.1016/j.tvjl.2025.106357","DOIUrl":"10.1016/j.tvjl.2025.106357","url":null,"abstract":"<div><div>To date, no intravenous pharmacokinetics (PK) studies have assessed acetaminophen or its major metabolites (acetaminophen-glucuronide and acetaminophen-sulphate) in horses. The influence of sex on acetaminophen metabolism in horses is unclear, and Monte Carlo simulations have not been applied to explore potential clinical applications. This study aimed to determine the intravenous PK and safety of acetaminophen in Andalusian horses at 10 and 20 mg/kg, evaluate sex as a covariate, and simulate clinical regimens. Twenty mares and twenty stallions received intravenous acetaminophen, and plasma concentrations were analysed by LC-MS/MS and modelled using non-linear mixed-effects models (MonolixSuite®). Safety was evaluated via clinical examinations and by haematological and biochemical profiles 24 h after administration. Two constant-rate infusion (CRI) regimens were examined in PK simulations that did not involve surgery: a short-duration, high-dose infusion (3 h) for peri-operative scenarios (short-infusion group at 3.33, 6.66, and 10.0 mg/kg/h) and a longer-duration, lower-dose infusion (8 h) for post-operative analgesia scenarios (long-infusion group at 1.25, 2.50, and 3.75 mg/kg/h). After modelling, glucuronide and sulphate levels were 2.1 and 4.53 times higher, respectively, than acetaminophen levels. Half-lives were 3.5, 5.6, and 3.2 h for acetaminophen, glucuronide, and sulphate, respectively. Clearance was influenced by sex (mares: 0.47 L/h/kg; stallions: 0.35 L/h/kg). No adverse effects were observed. Steady-state concentrations observed in the CRI simulations ranged from 1.75–5.21 µg/mL (short-infusion group) and 0.62–2.02 µg/mL (long-infusion group). Acetaminophen exhibited sex-dependent PK variability and high-level formation of metabolites. These findings support further clinical evaluation of acetaminophen CRIs in equine analgesia.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106357"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic analysis and spatiotemporal distribution of newly emerging lumpy skin disease virus in malaysia identify recombinant strain from transboundary spread 马来西亚新发结节性皮肤病病毒的基因组分析和时空分布确定了跨界传播的重组菌株

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-24 DOI: 10.1016/j.tvjl.2025.106365

Nurain Izzati Saidi, Nur Athirah Azhar, Mohamad Azlan Jahaya, Mohd. Shafarin Shamsuddin, Syahrol Zamzuri

{"title":"Genomic analysis and spatiotemporal distribution of newly emerging lumpy skin disease virus in malaysia identify recombinant strain from transboundary spread","authors":"Nurain Izzati Saidi, Nur Athirah Azhar, Mohamad Azlan Jahaya, Mohd. Shafarin Shamsuddin, Syahrol Zamzuri","doi":"10.1016/j.tvjl.2025.106365","DOIUrl":"10.1016/j.tvjl.2025.106365","url":null,"abstract":"<div><div>Lumpy skin disease is a transboundary viral disease affecting bovine species. This disease had never been recorded in Malaysia prior to May of 2021, and had spread to 65 of 92 districts in Peninsular Malaysia by December of the same year. In this study, we conducted a whole-genome sequencing, phylogenetic analysis and geographical mapping of the outbreak from lumpy skin disease virus isolated from the first wave of outbreaks to better understand the origin, transmission, and genetic diversity of the virus. The whole genome sequences of these isolates were deposited in GenBank with accession number PP145891 and PP145892. Malaysia LSDV isolates were found clustered with recombinant strains in clade 2.5, particularly subclade 2.5.1, which included strains from China, Vietnam, Thailand, and Indonesia. The spread of the disease in Malaysia was closely linked to cross-border cattle movements from Thailand, as evidenced by the spatiotemporal distribution of the outbreaks. This study emphasizes the need for ongoing genomic surveillance to detect emerging variants and inform targeted control strategies, including vaccination and cross-border cooperation to curb future outbreaks.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106365"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral fluid collection in wild boar: A field protocol 野猪口服液采集：一项野外试验方案

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-22 DOI: 10.1016/j.tvjl.2025.106362

David Relimpio , Aleksandra Kosowska , Sandra Barroso-Arévalo , Daniel De Antonio-Gómez , Christian Gortázar , Jose A. Barasona

{"title":"Oral fluid collection in wild boar: A field protocol","authors":"David Relimpio , Aleksandra Kosowska , Sandra Barroso-Arévalo , Daniel De Antonio-Gómez , Christian Gortázar , Jose A. Barasona","doi":"10.1016/j.tvjl.2025.106362","DOIUrl":"10.1016/j.tvjl.2025.106362","url":null,"abstract":"<div><div>Traditional wildlife sampling methods have limitations in providing a well-distributed, timely and accurate disease surveillance in wildlife. We developed a field protocol for collecting wild boar (<em>Sus scrofa</em>) oral fluid samples for non-invasive active disease surveillance using environmental ropes. We deployed ropes in field settings and tested the rope-sampled oral fluids for antibodies against <em>Mycobacterium bovis</em> and closely related members of the <em>Mycobacterium tuberculosis</em> complex (MTC). Oral fluid samples were collected from three wild boars populations in Spain with endemic tuberculosis across two seasons with contrasting natural food availability. Additionally, we studied the effect of placing the ropes close to aggregation points and of impregnating the ropes with corn powder. The results suggested that installing environmental ropes in summer, close to feeders or aggregation points, and baited with corn powder significantly increases the proportion of bitten ropes. No significant differences were observed in the effectiveness of ropes for oral fluid sampling across the three study sites. The average rate of antibody detection by ELISA was 38 % (range 34–45 %) of the bitten ropes. This method appears to be both precise and sensitive in detecting antibodies against MTC in oral fluid samples collected from environmental ropes, making it a potentially valuable tool for early detection and monitoring of MTC circulation. This noninvasive sampling method can easily be adapted for other relevant diseases of wild suids including African swine fever. Therefore, environmental ropes represent an effective, inexpensive, flexible, environmentally friendly, and noninvasive alternative for active wildlife disease surveillance.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106362"},"PeriodicalIF":2.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility of a simultaneous ultrasound (US) and peripheral nerve stimulation (PNS) guided interscalene brachial plexus injection in calves 超声（US）和周围神经刺激（PNS）同时引导斜角肌间臂丛注射的可行性

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-22 DOI: 10.1016/j.tvjl.2025.106360

B. Alonso , J. Viscasillas , L. Vlaminck , S. Schauvliege

{"title":"Feasibility of a simultaneous ultrasound (US) and peripheral nerve stimulation (PNS) guided interscalene brachial plexus injection in calves","authors":"B. Alonso , J. Viscasillas , L. Vlaminck , S. Schauvliege","doi":"10.1016/j.tvjl.2025.106360","DOIUrl":"10.1016/j.tvjl.2025.106360","url":null,"abstract":"<div><div>The feasibility of a simultaneous ultrasound and peripheral nerve stimulation (PNS) guided interscalene brachial plexus injection was investigated in 6 calves, using three different techniques (2 calves per group, bilateral injections). Group 1: one aliquot of 0.3 mL/kg administered near the ventral branches of the eighth cervical/first thoracic (C8-T1) nerves. Groups 2 and 3: two aliquots of respectively 0.15 mL/kg and 0.3 mL/kg, one administered near C8-T1 and one near the ventral branches of the sixth/seventh cervical (C6-C7) nerves. The calves were sedated (0.2 mg/kg xylazine intramuscularly and 2 mg/kg ketamine intravenously) and positioned in lateral recumbency. Following aseptic preparation, a linear ultrasound probe was positioned at the shoulder level, transversely oriented to the longitudinal axis of the first rib. The ventral branches of C8-T1 and C6-C7 were visualised between the scalene muscles, and a 20 gauge insulated needle connected to a PNS was inserted in-plane until near the target nerves. A dye solution was injected once the motor response was absent below 0.4 mA stimulus. The procedure was repeated in the contralateral limb, and the calves were euthanised (70 mg/kg pentobarbital intravenously). Dissection was performed to assess nerve staining (Not stained, Poor, Partial or Complete circumference with at least 1-centimetre length).</div></div><div><h3>Results</h3><div>C8-T1: 3/4 completely and 1/4 partially stained (all groups). C6-C7: 0/4; 2/4; 4/4 partially, 3/4; 2/4; 0/4 poor and 1/4; 0/4; 0/4 not stained in groups 1, 2 and 3, respectively. In conclusion, this technique seems feasible. Further research is needed to determine the ideal injection volume.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106360"},"PeriodicalIF":2.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The epidemiology of mammary tumours in insured female dogs in Sweden 瑞典有保险的母狗乳腺肿瘤的流行病学

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-18 DOI: 10.1016/j.tvjl.2025.106359

Karolina Engdahl , Henrik Rönnberg , Sara Saellström , Åke Hedhammar , Bodil Ström Holst

{"title":"The epidemiology of mammary tumours in insured female dogs in Sweden","authors":"Karolina Engdahl , Henrik Rönnberg , Sara Saellström , Åke Hedhammar , Bodil Ström Holst","doi":"10.1016/j.tvjl.2025.106359","DOIUrl":"10.1016/j.tvjl.2025.106359","url":null,"abstract":"<div><div>Mammary tumour (MT) is the most common neoplasia in bitches. This study explored the incidence and cause-specific mortality rates of and risk factors for MT in dogs insured by Agria Djurförsäkring in Sweden, 2011–2016. The study population included just over 600,000 dogs, and 13,109 females had veterinary care claims for MT. The incidence rate in females was 157 cases per 10,000 dog-years at risk (95 % confidence interval (CI): 154–160). The breeds at highest risk of MT were the Kerry blue terrier (relative risk (RR) 4.72, 95 % CI 2.58–7.92) and English springer spaniel (RR 3.32, 95 % CI: 3.02–3.63), while the breeds at lowest risk were the German spitz (RR 0.06, 95 % CI: 0.00–0.33) and English bulldog (RR 0.07, 95 % CI: 0.00–0.37). The median age at first MT diagnosis was 8.94 years (interquartile range (IQR) 7.43–10.5). The Dogue de Bordeaux (median age at diagnosis 5.30 years) and Irish wolfhound (6.42 years) were youngest at diagnosis, and the Miniature dachshund (10.9 years) and West Highland white terrier (11.0 years) were oldest. Higher odds of MT were found in bitches with a history of pseudopregnancy (OR 2.10, <em>P</em> < 0.001) and acute mastitis (OR 4.93, <em>P</em> < 0.001). In total, 486 bitches died of MT-related causes at a median age of 9.32 years (IQR 8.37–9.99). The Large Munsterlander had the highest risk of MT-related death (RR 40.3, 95 % CI: 8.28–118.4). In conclusion, MT mainly affected middle-aged to older bitches. Large breed-related differences in the risk of MT and age at diagnosis were found.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106359"},"PeriodicalIF":2.3,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Concurrent mucosal and transmural feline intestinal T-cell lymphomas show differing T-cell clonality 并发粘膜和跨壁猫肠t细胞淋巴瘤表现出不同的t细胞克隆性

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-16 DOI: 10.1016/j.tvjl.2025.106361

Masamine Takanosu , Yumiko Kagawa

{"title":"Concurrent mucosal and transmural feline intestinal T-cell lymphomas show differing T-cell clonality","authors":"Masamine Takanosu , Yumiko Kagawa","doi":"10.1016/j.tvjl.2025.106361","DOIUrl":"10.1016/j.tvjl.2025.106361","url":null,"abstract":"<div><div>The clonality of 14 feline intestinal small and large T-cell lymphomas were examined, which consisted of concurrent mucosal and transmural lymphomas, respectively. Histologically, the small cell lymphomas were localized to the mucosal region and were observed adjacent to large T-cell lymphoma lesions. The large T-cell lymphomas were spread throughout the submucosal region, forming transmural lesions. Both cell types were immunohistochemically stained using anti-cluster of differentiation 3 antibody. For clonality analysis, genomic DNA was extracted from formalin-fixed, paraffin-embedded sections of the mucosal and transmural lesions, separately. Clonality analysis was performed using primer sets targeting T cell receptor beta, T cell receptor delta<em>,</em> and T cell receptor gamma loci. In 12/14 cats, the results of the clonality analysis for T-cells differed between the mucosal and transmural lesions. Despite the fact that the cellular morphologies differed between lesion types, the T-cell clonality was consistent in 1/14 cats, suggesting a common clonal origin. In the remaining case, the clonal relationship between the 2 lesions could not be determined. These results indicate that concurrent lymphomas with small and large T-cells in their mucosal and transmural lesions, respectively, develop via separate pathogenic mechanisms.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106361"},"PeriodicalIF":2.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seaweed-derived mixed extracts exhibit immunomodulatory properties on porcine alveolar macrophages 海藻混合提取物对猪肺泡巨噬细胞具有免疫调节作用

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-15 DOI: 10.1016/j.tvjl.2025.106358

Sara Frazzini , Lauretta Turin , Graziella Vanosi , Luciana Rossi , Monika Hejna

{"title":"Seaweed-derived mixed extracts exhibit immunomodulatory properties on porcine alveolar macrophages","authors":"Sara Frazzini , Lauretta Turin , Graziella Vanosi , Luciana Rossi , Monika Hejna","doi":"10.1016/j.tvjl.2025.106358","DOIUrl":"10.1016/j.tvjl.2025.106358","url":null,"abstract":"<div><div>Antimicrobial resistance is a growing global concern, prompting for antibiotic alternatives in animal production. Seaweed, abundant in bioactive compounds with anti-inflammatory properties, offers a natural substitute to synthetic compounds. Considering this, the objective of the present study was to evaluate the anti-inflammatory bioactivity of three seaweeds 1:1 combination of <em>Ascophyllum nodosum</em>, <em>Palmaria palmata</em>, and <em>Ulva lactuca</em>. Initially, polyphenol, flavonoid, and total phlorotannin content of the three seaweed species were assessed through colorimetric assays. Subsequently, the anti-inflammatory bioactivity was first evaluated through an inhibition protein precipitation assay and then confirmed <em>in vitro</em> through gene expression assays in LPS-stimulated porcine alveolar macrophages (PAMs). The evaluation of the bioactive molecules revealed a high content of TPC (1487.67 ± 40.39 and 1763.57 ± 69.01 mg TAE/100 mg of sample, respectively), as well as of TFC (95.68 ± 3.62 and 126.09 ± 7.34 mg CE/100 mg of sample) and TPhC (0.167 ± 0.02 and 0.23 ± 0.01 mg PGE/100 mg) for AN and UL, respectively. The assay for inhibiting protein precipitation disclosed that the extracts combining two algae species (ANUL, ANPP, PPUL) were more effective than the effect exhibited by each single extract. The assessment of anti-inflammatory bioactivity revealed a significant down-regulation of IL-1β and TNF-α in the algae combination extracts. In contrast, TGF-β showed an increasing trend. These findings, along with confirmation of the high content of bioactive molecules, highlight the algae’s anti-inflammatory potential, making them suitable as natural alternatives to antibiotics for disease prevention in the livestock sector. Therefore, future research should explore the specific bioactive compounds and validate their efficacy <em>in vivo</em> to confirm their potential use in animal production.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106358"},"PeriodicalIF":2.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visual recombinase aided amplification technology for detecting feline coronavirus 视觉重组酶辅助扩增技术检测猫冠状病毒

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-12 DOI: 10.1016/j.tvjl.2025.106356

Xiaoman Lu , Yilin Cao , Ping Zhang , Xiaoyuan Chen , David M. Irwin , Yongyi Shen

{"title":"Visual recombinase aided amplification technology for detecting feline coronavirus","authors":"Xiaoman Lu , Yilin Cao , Ping Zhang , Xiaoyuan Chen , David M. Irwin , Yongyi Shen","doi":"10.1016/j.tvjl.2025.106356","DOIUrl":"10.1016/j.tvjl.2025.106356","url":null,"abstract":"<div><div>Feline Coronavirus (FCoV) is responsible for causing intestinal lesions and potentially fatal systemic infections in cats, with a worldwide prevalence. Currently, there is no effective vaccine available, making early diagnosis essential for treatment. Although RT-PCR detection is known for its high specificity and accuracy, the method involves complex experimental procedures and necessitates costly equipment, which restricts its broader clinical use. In this study, we utilized Recombinase Aided Amplification (RAA) technology in conjunction with a nucleic acid-colloidal gold immunochromatographic test strip to establish a visual detection method that can rapidly and accurately identify FCoV and differentiate between its two serotypes, serotype I and serotype II. Primers and probes were designed based on conserved sequences from the N gene and the S1 gene. Under isothermal conditions at 39°C, results can be obtained in just 30 minutes. This method demonstrates no cross-reactivity with other prevalent infectious viruses in felines, such as feline calicivirus (FCV), feline panleukopenia virus (FPV), and feline herpesvirus type 1 (FHV-1). In comparison to qRT-PCR, which has a detection limit of approximately 100 copies/μL, the RAA method exhibits significantly enhanced sensitivity. The detection limits for the FCoV N gene and the S1 gene serotypes I and II were determined to be 15.5 copies/μL, 1.97 copies/μL, and 1.46 copies/μL, respectively. Preliminary clinical applications have shown that the results align perfectly with those obtained from RT-PCR detection, achieving 100 % consistency. In summary, this novel detection method offers high accuracy, a brief reaction time, robust specificity, elevated sensitivity, and is straightforward and convenient to implement.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106356"},"PeriodicalIF":2.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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