Veterinary journal最新文献

{"title":"Drug resistance detection of canine origin Escherichia coli in China and inhibition by genipin","authors":"Nan Niu ,&nbsp;Lei-Na Dou ,&nbsp;Shuo Yang ,&nbsp;Hai-Xin Wang ,&nbsp;Shen Zhuang ,&nbsp;Yun-Peng Fan ,&nbsp;Ying- Qiu Liu ,&nbsp;Wei-Min Zhang ,&nbsp;Wu-Ren Ma","doi":"10.1016/j.tvjl.2025.106307","DOIUrl":"10.1016/j.tvjl.2025.106307","url":null,"abstract":"<div><div>Zoonotic transmission from pets to their owners is a major health problem. It is important to determine and control the drug resistance in pets to mitigate risks of human transmission. In this work, the current prevalence of multi-drug resistance (MDR) and resistance gene in <em>Escherichia coli</em> (<em>E. coli</em>) derived from dogs in nine cities across various regions of China initially evaluated using microfluidic dilution methods and polymerase chain reaction (PCR) technology. To control antibiotic resistance, genipin as natural product was used to combat MDR <em>E. coli.</em> Finally, the synergistic effect of genipin and norfloxacin on MDR <em>E. coli</em> was studied using time-kill curves to retard the resistance spread. A total of 126 <em>E. coli</em> strains were isolated from 154 collected fecal samples of dogs. Minimum inhibitory concentrations (MIC) results revealed that the highest detection rate of MDR <em>E. coli</em> appeared in Zhengzhou at 90.9 %, and the lowest in Shenyang at 10.0 %. The results of drug resistance gene testing indicated that the <em>bla</em>_<em>TEM</em> gene had the highest detection rate (99.2 %), then <em>tetA</em> and <em>bla</em>_{<em>CTX-M-1</em>}, whose detection rates all exceed 50 %. Furthermore, the MIC of genipin against MDR <em>E. coli</em> was found to be 4096 μg/mL, and genipin at ½ MIC demonstrated significant inhibition on MDR <em>E. coli</em> within 6 h. Finally, the combination of ¼ MIC genipin with ½ MIC norfloxacin showed partial synergistic inhibitory effect on MDR <em>E. coli</em>. Our findings suggest that although antibiotic resistance in canine origin <em>E. coli</em> varies across different regions of China, it remains concerning, and genipin shows potential as a treatment option for MDR <em>E. coli</em> infections.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106307"},"PeriodicalIF":2.3,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143075721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of sedation on haematological, biochemical, coagulation profile, and kaolin-activated thromboelastography in rabbits","authors":"Tiziana Bassan ,&nbsp;Josep Pastor ,&nbsp;Javier Martinez-Caro ,&nbsp;Beatriz Agulla ,&nbsp;Jaume Martorell","doi":"10.1016/j.tvjl.2025.106298","DOIUrl":"10.1016/j.tvjl.2025.106298","url":null,"abstract":"<div><div>Blood sampling and analysis are essential procedures for assessing the health status of exotic pets. While careful manual restraint is generally recommended, sedation may be necessary in specific cases. However, the use of chemical restraint may introduce analytical variations. The aim of this study is to determine the effects of sedation with butorphanol, midazolam, ketamine and dexmedetomidine on haematological, biochemical and coagulation parameters (prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen), as well as thromboelastography in adult healthy New Zealand rabbits (<em>Oryctolagus cuniculus</em>). Two groups of adult New Zealand rabbits, housed under identical conditions and considered healthy based on normal physical examination and unremarkable clinical histories, were included in the study. The non-sedated group comprised ten rabbits, while the sedated group consisted of fifteen. Blood samples were collected via jugular venipuncture, and haematological, biochemical and coagulation profiles were performed. In conclusion, some variation in haematological and biochemical values were observed depending on sex and/or sedation protocols. Sex can influence haematology in terms of haemoglobin concentration, lymphocyte and platelet count; biochemistry in alanine aminotransferase, chloride, gamma-glutamyl transferase, potassium, sodium, calcium, total cholesterol, urea nitrogen, but not in coagulation parameters. Meanwhile, sedation can affect haematology in terms of leucocyte and lymphocyte count and biochemistry in total bilirubin, calcium, total protein, sodium, albumin, glucose, creatinine, phosphorous. Plasma-based coagulation assays showed increased prolongation of aPTT and PT, although these changes do not seem to be clinically relevant. There were no changes observed in thromboelastographic parameters.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106298"},"PeriodicalIF":2.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143041899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bovine adenovirus prevalence and its role in bovine respiratory disease complex: A systematic review and meta-analysis","authors":"Gebremeskel Mamu Werid ,&nbsp;Yassein M. Ibrahim ,&nbsp;Gebrerufael Girmay ,&nbsp;Farhid Hemmatzadeh ,&nbsp;Darren Miller ,&nbsp;Roy Kirkwood ,&nbsp;Kiro Petrovski","doi":"10.1016/j.tvjl.2025.106303","DOIUrl":"10.1016/j.tvjl.2025.106303","url":null,"abstract":"<div><div>Bovine adenoviruses (BAdVs) are major contributors to the bovine respiratory disease complex (BRDC). A systematic review and meta-analysis were carried out to explore the epidemiology of BAdV across diverse cattle populations using different detection methods. The study showed a higher BAdV prevalence of 0.66 in general cattle populations using antibody detection, compared with 0.28 in cattle showing clinical signs. The study identified significant prevalence differences between BAdV-3 (0.87) and BAdV-7 (0.21) in general cattle populations. However, in clinical cattle, BAdV-3 and BAdV-7 showed similar prevalence at 0.27 and 0.32, respectively. Moreover, a high herd-based BAdV seroprevalence of 0.82 was observed. When nucleic acid detection methods were used in general cattle populations, a lower BAdV (0.05) prevalence was observed, in contrast to the higher prevalence (0.32) in cattle exhibiting clinical signs. In contrast, using antigen detection in cattle with clinical signs of disease showed a prevalence of 0.06, compared to 0.32 with nucleic acid methods, indicating detection method-specific sensitivity and specificity. The study also highlighted the role of BAdV in BRDC, particularly BAdV-3 and BAdV-7. Existing empirical evidence on BAdV epidemiology and pathobiology is scarce and requires further investigation; however, the current findings offer insights into the epidemiology of BAdV and its role in the BRDC, which could potentially inform and enhance disease control strategies.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106303"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central nervous system vascular complications associated with the acute form of steroid-responsive meningitis-arteritis","authors":"C. Mayor ,&nbsp;C. de La Fuente ,&nbsp;A. Pereira ,&nbsp;J. Viu ,&nbsp;S. Añor","doi":"10.1016/j.tvjl.2025.106304","DOIUrl":"10.1016/j.tvjl.2025.106304","url":null,"abstract":"<div><div>This retrospective study aims to describe the vascular events in the central nervous system (CNS) associated with the acute form of steroid-responsive meningitis-arteritis (SRMA), to compare the clinical features of dogs with and without such complications and to potentially identify predisposing factors for these events. Dogs with a presumptive diagnosis of SRMA visited between 2018 and 2023 with full medical records that underwent neurological examination, blood testing, cervical computed tomography or magnetic resonance imaging and cerebrospinal fluid (CSF) analysis were included.</div><div>Thirty-three dogs were included and divided in two groups. Group 1 included 7 (21,2 %) dogs with vascular complications secondary to SRMA (spinal cord ischemic and/or hemorrhagic infarcts, spinal cord subdural hematomas, intracranial subarachnoid hemorrhages), and group 2 included 26 (78,8 %) dogs with non-complicated SRMA. Age, breed, sex, presence of neurological deficits, CSF abnormalities and presence of relapses were factors evaluated for potential association with vascular complications of SRMA.</div><div>Six dogs in group 1 were Golden Retrievers (85,7 %), and there was a significant association between this breed and the occurrence of vascular complications (<em>P</em> = 0.017). Presence of neurological deficits (<em>P</em> = 0.001) and xanthochromic CSF (<em>P</em> = 0.001) were also associated with vascular complications in dogs with SRMA.</div><div>Hemorrhagic or ischemic lesions in the CNS can be a complication of the acute form of SRMA, and Golden Retrievers appear to be more affected. Dogs with vascular complications show often neurological deficits and CSF xanthochromia.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106304"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance and genetic diversity of bovine viral diarrhea virus in dairy herds across Taiwan","authors":"Fong-Yuan Lin ,&nbsp;Hau-You Tzeng ,&nbsp;Ching-Yu Tseng ,&nbsp;Ruei-Sheng Tsai ,&nbsp;Mami Oba ,&nbsp;Tetsuya Mizutani ,&nbsp;Yumiko Yamada ,&nbsp;Hue-Ying Chiou ,&nbsp;Shih-Te Chuang ,&nbsp;Wei-Li Hsu","doi":"10.1016/j.tvjl.2025.106305","DOIUrl":"10.1016/j.tvjl.2025.106305","url":null,"abstract":"<div><div>Bovine viral diarrhea virus (BVDV) significantly impacts cattle worldwide, causing respiratory, gastrointestinal, and reproductive disorders that lead to substantial economic losses. Despite its high global prevalence and various genotypes, the infection status of BVDV in Taiwan has not been reported. This study conducted large-scale surveillance in 2014, analyzing 460 bovine serum samples collected from 49 dairy herds across Taiwan. The results revealed a herd-level seroprevalence of 59.2 % (29/49), with significant regional variations: 16.7 % in the northern region and 77.8 % in the southern region. At the animal level, the overall BVDV-positive rate was 32.4 % (148/460), ranging from 3.3 % (northern region) to 41.5 % (central region), with significant regional differences as analyzed by the mixed-effects logistic regression model. Five-year surveillance of a single dairy farm revealed persistent BVDV circulation, with the seroprevalence starting at 89 % in 2019 and remaining between 82 % and 100 % among the cohort of 27 cattle monitored from 2020 to 2023. In contrast, a study of three farms revealed that while the two high-prevalence farms maintained high infection rates, the low-prevalence farm experienced a gradual decline in infections, indicating varied infection dynamics. Moreover, this study identified BVDV-1b as the predominant genotype in Taiwan, along with BVDV-1a and BVDV-2a, which were detected in mosquito samples. These findings emphasize the high prevalence of BVDV in Taiwan, ongoing viral circulation within herds, and the need for continuous surveillance and robust control measures to mitigate the spread of BVDV in Taiwan's cattle industry.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106305"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum macromineral dynamics and milk production of old Holstein cows submitted to a postpartum delayed milking strategy","authors":"H. Beiranvand ,&nbsp;A.A. Alamouti ,&nbsp;R. Rostami ,&nbsp;S.R. Majidi Monfared ,&nbsp;M.R. Bakhtiarizadeh ,&nbsp;B. Khorrami ,&nbsp;M. Yazdanyar ,&nbsp;F. Ahmadi","doi":"10.1016/j.tvjl.2025.106300","DOIUrl":"10.1016/j.tvjl.2025.106300","url":null,"abstract":"<div><div>Lactation initiates with a massive Ca secretion into milk. Within 24–48 h post-calving, high-producing, older-parity dairy cows are highly susceptible to Ca disturbances. We hypothesized that the abrupt cessation of milking within this critical period would delay Ca secretion into milk, allowing lactating cows more time to stabilize their Ca homeostasis mechanisms and potentially lower the risk of blood Ca decline in the immediate postpartum period. After colostrum harvest, a total of 175 multiparous Holsteins (parity ranging from 3 to 7) were randomly assigned to three treatment groups: 1) control (n = 59), whereby cows were milked 3 times/day (M0), 2) the treatment whereby milking of cows was withdrawn for the first 24 h (M24; n = 60), and 3) for the first 48 h after calving (M48; n = 56). All cows were given two oral Ca boluses (31 g Ca/bolus), the first bolus was administered within 30–60 min after calving, and the second bolus was given 24 h later. After the delayed milking treatment, cows in M24 and M48 groups returned to M0 milking program. On d 2 and 3 post-calving, blood total Ca concentration was not different between M24 and M48 cows, but both groups had greater Ca concentrations than M0 cows. On day 2 post-calving, serum P concentration was greater in M48 compared to M24 and M0 (1.86 vs. 1.47 ± 0.06 mmol/L). On d 3, M0 cows had lower P concentration than M24 or M48 cows. Despite a transient decline in milk production in M24- and M48-treated cows, both treatments attained a similar milk production level as they switched to M0 milking program. No difference existed in the first 3 monthly test-day milk records. The postpartum milking strategy had no impact on the culling risk by 120 DIM. These results support delaying milking for 24 and 48 h after colostrum collection to maintain Ca status in multiparous cows. Evaluation of the long-term effects of the delayed milking strategies on mammary health, reproduction performance, and survivability of pluriparous dairy cows is recommended.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106300"},"PeriodicalIF":2.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological analysis of mammary tumors in female dogs in Japan: A study based on Kyushu–Okinawa region","authors":"Shinji Hirano ,&nbsp;Tatsuro Hifumi ,&nbsp;Noriaki Miyoshi","doi":"10.1016/j.tvjl.2025.106301","DOIUrl":"10.1016/j.tvjl.2025.106301","url":null,"abstract":"<div><div>Canine mammary tumors (CMTs) are common tumors in female dogs (FDs), and at least nearly half of these lesions of malignant. We examined the epidemiology of CMTs in Japan using excisional biopsy cases (<em>n</em> = 7802) collected from 2005 to 2023 in the Kyushu–Okinawa region. We investigated the prevalence, effects of breed, neutering, and age on CMT and malignant CMT (mCMT) risk through general statistics and multivariate analyses. The distribution of CMT histological types was also compared among different breeds and mixed breeds. In the Cohort (<em>n</em> = 6197) consisting of cases from primary veterinary hospitals, the numbers of CMT and mCMT cases (2928 and 822 cases, respectively) and the adjusted prevalence is ranged 4.76–8.09 per 1000 dogs and increasing over time (<em>P</em> &lt; 0.001). A multivariate model identified breeds with high or low risks of CMT or mCMT. Neutered FDs had lower risk of CMT than intact FDs (risk ratio = 0.57, 95 %CI: 0.53–0.61). Compared to the age with the highest incidence, those aged ≥ 8 and ≥ 14 years had comparable rates of CMT and mCMT, respectively. Certain breeds exhibited biases regarding CMT histological types compared to mixed breeds. This first epidemiological analysis of CMT in Japan will be a valuable resource for CMT control.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106301"},"PeriodicalIF":2.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence of burnout in veterinary medicine: Exploring interventions to improve well-being","authors":"Yoshiyasu Takefuji","doi":"10.1016/j.tvjl.2025.106299","DOIUrl":"10.1016/j.tvjl.2025.106299","url":null,"abstract":"<div><div>The veterinary profession faces a critical challenge: burnout. Long hours, emotional strain, financial pressures, and difficult client interactions contribute to stress and drive veterinary professionals from the field. This harms not only their well-being but also patient care and workplace morale. Research highlights the concerning mental state of veterinarians, with studies finding high rates of burnout, compassion fatigue, and burden transfer (stress from client challenges). Early-career and female veterinarians are most vulnerable. Several studies explore interventions to improve veterinary well-being. Educational programs targeting communication and acceptance of difficult clients show promise in reducing stress and burnout. Additionally, a web-based acceptance and commitment therapy (ACT) program demonstrates effectiveness in reducing occupational distress. It is crucial to address the veterinary burnout crisis. By creating supportive work environments, prioritizing mental health, and implementing interventions like ACT training, we can retain veterinary professionals and ensure a thriving profession that provides optimal care for animals and their caregivers.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"310 ","pages":"Article 106299"},"PeriodicalIF":2.3,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast growth factor 23 – A review with particular reference to the physiology and pathophysiology of phosphate homeostasis in the cat","authors":"Jonathan Elliott,&nbsp;Pak Kan Tang","doi":"10.1016/j.tvjl.2024.106271","DOIUrl":"10.1016/j.tvjl.2024.106271","url":null,"abstract":"<div><div>Fibroblast growth factor-23 (FGF23) is a phosphaturic hormone, discovery of which has transformed our understanding of mineral regulation in healthy mammals, including the cat. It is produced by osteoblasts and osteocytes and its prime role is to regulate phosphate entry into extracellular fluid (from bone and via the gut) and its excretion via the kidney. It interacts with other hormones (calcitriol and parathyroid hormone), inhibiting their activation and secretion respectively and so impacts on calcium as well as phosphate homeostasis. Physiological factors regulating its secretion are not well understood, although phosphate ion sensing is likely to be important. Calcium and magnesium ions are also involved and unravelling the control points and integration of the system regulating bone turnover and mineral balance whilst preventing soft tissue (non-osseous) mineralisation is a future research goal. Calciprotein particle size and number likely play an important role in this system but precisely how remains to be determined. Elevated serum FGF23 is the earliest indicator of mineral bone disorder associated with chronic kidney disease in human patients and in cats, enabling reference-range serum phosphorus to be maintained despite reduction in glomerular filtration rate which limits phosphate excretion. FGF23 also predicts CKD progression and survival in cats. The many factors influencing its secretion at different stages of CKD, including relative iron deficiency, anaemia and chronic systemic inflammation, hypomagnesaemia and α-klotho deficiency are discussed in this review, where the data available in cats with naturally occurring CKD is presented alongside that from rodent models and human CKD patients.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"309 ","pages":"Article 106271"},"PeriodicalIF":2.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of brain injury in cattle with Theileria annulata: Neuron-specific biomarkers, inflammation, oxidative stress and apoptosis","authors":"M. Uztimür ,&nbsp;C.N. Ünal ,&nbsp;M.B. Dörtbudak ,&nbsp;R. Fırat ,&nbsp;A.İ. Ekinci","doi":"10.1016/j.tvjl.2024.106269","DOIUrl":"10.1016/j.tvjl.2024.106269","url":null,"abstract":"<div><div>This study aimed to investigate selected brain-specific biomarkers in cattle with tropical theileriosis caused by <em>Theileria annulata</em> (<em>T. annulata</em>) and to evaluate their diagnostic and prognostic significance. The study group consisted of 25 cattle naturally infected with <em>T. annulata</em>, while the control group consisted of 10 healthy cattle. Animals with <em>T. annulata</em> were classified according to hematocrit (HCT) value as severe anemia group 1 (<em>n</em>:12) with HCT ≤12 and moderate anemia group 2 (<em>n</em>:13) with HCT between 13 and 24. Histopathological and immunohistochemical examinations of the brain tissue were performed in 10 nonsurvivor cattle. Serum calcium-binding protein B (S100B), glial fibrillary acidic protein (GFAP), tau protein and ubiquitin C-terminal hydrolase-1 (UCHL-1) concentrations in brain injury were measured using bovine-specific ELISA kits. S100B, GFAP, and Tau concentrations of cattle in the <em>T. annulata</em> group were found to be significantly higher than the control group (<em>P</em>&lt;0.001). Brain specific biomarkers showed significant correlations with erythrocyte count, HCT and bilirubin. The presence of brain damage was confirmed by histopathological and immunohistochemical techniques. The results of ROC analysis showed that S100B with AUC value of 0.88 and GFAP with an AUC value of 0.82 were significant prognostic indicators. Additionally, S100B, GFAP and Tau showed significant diagnostic performance with an AUC value of 0.88, 0.92 and 0.86, respectively. In conclusion, brain-specific biomarkers can be used as diagnostic and prognostic markers in the assessment of brain damage in cattle naturally infected with T. annulata.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"309 ","pages":"Article 106269"},"PeriodicalIF":2.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}