Veterinary journal最新文献

{"title":"Comparison of equine-induced pluripotent stem cell characteristics induced on different cell adhesion substrates","authors":"Chiho Kushida ,&nbsp;Tatsuya Usui ,&nbsp;Norihisa Tamura ,&nbsp;Yoshinori Kasashima ,&nbsp;Kota Sato ,&nbsp;Katsuhiko Arai","doi":"10.1016/j.tvjl.2025.106351","DOIUrl":"10.1016/j.tvjl.2025.106351","url":null,"abstract":"<div><div>This study evaluated the effects of cell adhesion substrates that lead to the generation of equine-induced pluripotent stem cells (eiPSC) from embryonic skin fibroblasts by lipofection of plasmid vectors expressing five reprogramming factors. The reprogramming efficiency of cells induced on the E8 fragment of laminin-511 (eiPSC-511) was higher than that on Geltrex containing laminin-111 as a major laminin (eiPSC-111), and supplementation with a cocktail of small molecular compounds increased the number of iPSC colonies on both substrates. In the cell proliferation assay, eiPSC-511 showed higher growth activity than eiPSC-111. Although no significant changes were observed in the expression of pluripotency markers between eiPSC-111 and eiPSC-511, the expression of DPPA3 was significantly upregulated in both iPSCs by reprogramming, suggesting that DPPA3 was a sensitive pluripotent marker for equine iPSC. While both iPSCs expressed high mRNA level of integrin alpha6 and beta1 subunits, mRNA level corresponding to ITGA3 and ITGA7 significantly increased in eiPSC-511 in comparison to those in eiPSC-111. These results suggested that the binding strength to the substrate in eiPSC-511 was stronger than that in eiPSC-111. On the contrary, although no significant differences were observed in the histology of teratomas, increased <em>in vitro</em> differentiation into three germ layers in eiPSC-111 was shown compared to those in eiPSC-511. Thus, these results contributed to the improved generation of iPSC in horses.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106351"},"PeriodicalIF":2.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical presentation, diagnostic findings, and outcome in dogs with presumptive primary and reactive immune-mediated polyarthritis in France","authors":"Mario Cervone,&nbsp;Luc Chabanne,&nbsp;Émilie Krafft,&nbsp;Jean-Luc Cadoré","doi":"10.1016/j.tvjl.2025.106355","DOIUrl":"10.1016/j.tvjl.2025.106355","url":null,"abstract":"<div><div>Limited data exist regarding immune-mediated polyarthritis in dogs, particularly regarding reactive immune-mediated polyarthritis. The aim of this study was to describe the clinical presentations, the diagnostic findings, and the outcome of dogs diagnosed with presumptive primary and reactive immune-mediated polyarthritis in France, and to evaluate potential biomarkers for assessing treatment response. Medical records were retrospectively reviewed for dogs diagnosed with immune-mediated polyarthritis between June 2004 and January 2020. Fifty-eight dogs were included, of which 43 diagnosed with primary and 15 with reactive immune-mediated polyarthritis. Associated diseases in dogs with reactive immune-mediated polyarthritis included leishmaniosis (7), digestive disorders (3), eosinophilic bronchopneumopathy (3), bacterial infection (1), and gossypiboma (1). Overall, dogs showed a combination of joint swelling, pain, or heat. Anaemia (30 %), leucocytosis (47 %), high serum CRP (90 %) and low serum albumin (61 %) concentrations were the most frequent bloodwork abnormalities. Most dogs (74 %) experiencing complete clinical remission, and the mean time to remission was 37 days. Serum albumin, total proteins and globulins concentrations appeared as potential predictors for the time to remission. Clinical presentation and diagnostic features are not specific in dogs with immune-mediated polyarthritis. Overall, most dogs achieve clinical remission, but the time to achieve remission may be long. Serum albumin, total proteins and globulins concentrations may be predictors for the time to remission.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106355"},"PeriodicalIF":2.3,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143834059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the genetic influences on equine analgesic efficacy through genome-wide association analysis of ranked pain responses","authors":"Elouise K. Bacon ,&nbsp;Callum G. Donnelly ,&nbsp;Carrie J. Finno ,&nbsp;Bianca Haase ,&nbsp;Brandon D. Velie","doi":"10.1016/j.tvjl.2025.106347","DOIUrl":"10.1016/j.tvjl.2025.106347","url":null,"abstract":"<div><div>Multimodal analgesic administration is a promising strategy for mitigating side effects typically associated with analgesia; nevertheless, variation in analgesic effectiveness still poses a considerable safety concern for both horses and veterinarians. Pharmacogenomic studies have started delving into genetic influences on varying drug effectiveness and related side effects. However, current findings have narrow implications and are limited in their ability to individualize analgesic dosages in horses. Hydromorphone and detomidine were administered to a cohort of 48 horses at standardized time intervals, with dosage rates recorded. Analgesic effectiveness was scored (1−3) based on pain response to dura penetration during cerebrospinal fluid centesis. Genome-wide association (GWA) analyses identified two SNVs passing the nominal significance threshold (P &lt; 1 ×10⁻⁵) in association with analgesic effectiveness. One SNV identified on chromosome 27 (rs1142378599) is contained within the <em>LOC100630731 disintegrin and metalloproteinase domain-containing protein 5</em> gene. The second identified SNV is an intergenic variant located on chromosome 29 (rs3430772468) These SNVs accounted for 26.11 % and 31.72 % of explained variation in analgesic effectiveness respectively, with all eight of the horses with the lowest analgesic effectiveness expressing the A/C genotype at rs3430772468, with six of which also expressing the C/T genotype at rs1142872965. Whilst highlighting the multifactorial nature of analgesic efficacy, this study serves as an important step in the application of genome-wide approaches to better understand genetic factors underpinning commonly observed variation in analgesic effectiveness in horses, with the goal of tailoring analgesic dosage to minimize commonly observed side effects and improve the outcomes of equine pain management.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106347"},"PeriodicalIF":2.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a cell culture system from bovine and bubaline endometrial cells recovered by a minimally invasive technique using a cytobrush","authors":"Ruchikon Jongsuwanwattana ,&nbsp;Sudson Sirivaidyapong ,&nbsp;Theerawat Swangchan-Uthai","doi":"10.1016/j.tvjl.2025.106353","DOIUrl":"10.1016/j.tvjl.2025.106353","url":null,"abstract":"<div><div>Ruminant endometrial cell culture is a common model system used to study uterine receptivity and related signaling in the maternal endometrium and pregnancy. The present study aimed to develop a minimally invasive technique by employing a cytobrush for collecting endometrial cell samples retrieved from cattle and buffalo and to establish and characterize the cells. The study was divided into two experiments. Experiment I evaluated the tissue invasiveness associated with different brush cytology rotations for 3, 5, 7, and 9 rounds compared with biopsy samples. In total, 30 areas of endometrial tissue samples from the uteri of six fresh, healthy, non-pregnant bovines from a slaughterhouse were examined histologically to determine tissue invasiveness based on the perpetual endometrial layer. The seven rounds of cytobrush rotation provided optimal results with detached epithelial cell lining and sloughed-off basement membrane while maintaining intact endometrial stroma, suggesting its suitability for subsequent cell culture. In Experiment II, primary endometrial cell cultures were established employing the 7-round brush cytology method from ten healthy, Holstein Friesian and swamp buffalo cows (n = 5, each). Endometrial cells were subcultured up to the third passage for further validation. Physical characterization utilized immunocytochemistry with pan-cytokeratin and vimentin co-staining. The cultured cells were functionally assessed by quantifying prostaglandin F2 alpha (PGF2α) secretion following 0 and 100 nM oxytocin challenge. Our findings showed that cytobrush sampling yielded sufficient seeding cells for culture. The proportions of endometrial epithelial cells relative to the stromal cells at passage 3 were 89.98 ± 7.21 % and 85.18 ± 2.66 % for cows and buffaloes, respectively. The secretion of PGF2α at 24 h increased significantly in bovine endometrial cell culture with 100 nM oxytocin (931.37 ± 292.69 pg/mL) compared with 0 nM oxytocin (194.06 ± 43.95 pg/mL; <em>p</em> = 0.026), as well as in bubaline endometrial cell culture, at 100 nM oxytocin (5.17 ± 1.54 pg/mL) compared 0 nM oxytocin (1.92 ± 0.76 pg/mL; <em>p</em> &gt; 0.05). In conclusion, minimally tissue-invasive <em>in vivo</em> brush cytology sampling methods are effective for establishing a primary endometrial cell culture system in cows and buffaloes, providing a valuable model for studying reproductive physiology.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106353"},"PeriodicalIF":2.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socio-geographic and demographic analysis of the official national registry data of dogs’ population in Portugal in 2023. Data from SIAC","authors":"Katia Pinello ,&nbsp;Helena Geraz ,&nbsp;Helena Sofia Salgueiro ,&nbsp;Eurico Cabral ,&nbsp;Emanuel Vieira ,&nbsp;Denisa Mendonça ,&nbsp;Milton Severo ,&nbsp;Ana Isabel Ribeiro ,&nbsp;João Niza-Ribeiro","doi":"10.1016/j.tvjl.2025.106349","DOIUrl":"10.1016/j.tvjl.2025.106349","url":null,"abstract":"<div><div>National data on dog populations has been historically scarce, hindering effective policy and welfare efforts. The SIAC (Information System for Companion Animals) registry now provides a robust dataset for analyzing dog demographics in Portugal. This study examines SIAC data (2004–2023), analyzing breed, sex, reproductive status, age, and geographic distribution. Chi-square tests with post-hoc residual analysis identified significant demographic and breed variations across districts, while clustering based on standardized residuals grouped districts by distinct profiles. Geographic and socioeconomic distributions were assessed using the European Deprivation Index and urbanicity classification, with spatial visualizations generated in R and QGIS. The dataset includes 2,581,870 dogs, with a predominance of younger dogs (ages 2–3 years), a slight male majority (51.6 %), and 54.4 % neutered. Mixed-breed dogs were most common (39.6 %), followed by Portuguese Podengo (10.9 %) and Labrador Retriever (6.1 %). Age distributions varied geographically, with younger dogs in rural areas and older dogs in urban settings. Rural regions had higher dog-to-household ratios, while urban and economically deprived areas had higher absolute dog numbers but lower ratios. The findings establish baseline data for canine studies and highlight social patterns in the dog population. They underscore the role of rural areas in preserving native Portuguese breeds and the need for targeted public health and veterinary initiatives. By integrating demographic, geographic, and socioeconomic factors, this study provides key insights to inform public health and animal welfare policies, advocating for tailored strategies to meet the diverse needs of urban and rural canine populations.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106349"},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerometer-derived classifiers for early detection of degenerative joint disease in cats","authors":"A.X. Montout ,&nbsp;E. Maniaki ,&nbsp;T. Burghardt ,&nbsp;M.J. Hezzell ,&nbsp;E. Blackwell ,&nbsp;A.W. Dowsey","doi":"10.1016/j.tvjl.2025.106352","DOIUrl":"10.1016/j.tvjl.2025.106352","url":null,"abstract":"<div><div>Decreased mobility is a clinical sign of degenerative joint disease (DJD) in cats, which is highly prevalent, with 61 % of cats aged six years or older showing radiographic evidence of DJD. Radiographs can reveal morphological changes and assess joint degeneration, but they cannot determine the extent of pain experienced by cats. Additionally, there is no universal objective assessment method for DJD-associated pain in cats. Developing an accurate evaluation model could enable earlier treatment, slow disease progression, and improve cats’ well-being. This study aimed to predict early signs of DJD in cats using accelerometers and machine learning techniques. Cats were restricted to indoors or limited outdoor access, including being walked on a lead or allowed into enclosed areas for short periods. Fifty-six cats were fitted with collar-mounted sensors that collected accelerometry data over 14 days, with data from 51 cats included in the analysis. Cat owners assessed their cats’ mobility and assigned condition scores, validated through clinical orthopaedic examinations. The study group comprised 24 healthy cats (no owner-reported mobility changes) and 27 unhealthy cats (owner-reported mobility changes, suggestive of early DJD). Data were segmented into 60-second windows centred around peaks of high activity. Using a Support Vector Machine (SVM) algorithm, the model achieved 78 % (confidence interval: 0.65, 0.88) area under the curve (AUC), with 68 % sensitivity (0.64, 0.77) at 75 % specificity (0.68, 0.79). These results demonstrate the potential of accelerometry and machine learning to aid early DJD diagnosis and improve management, offering significant advances in non-invasive diagnostic techniques for cats.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106352"},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study","authors":"Paweł Antoni Kołodziejski ,&nbsp;Natalia Leciejewska ,&nbsp;Maciej Sassek ,&nbsp;Leszek Nogowski ,&nbsp;Małgorzata Szumacher-Strabel ,&nbsp;Robert Mikuła ,&nbsp;Maciej Gogulski ,&nbsp;Ewa Pruszyńska-Oszmałek","doi":"10.1016/j.tvjl.2025.106354","DOIUrl":"10.1016/j.tvjl.2025.106354","url":null,"abstract":"<div><div>Adipose tissue functions as an endocrine organ; however, excessive lipid accumulation can lead to obesity and metabolic disorders, such as Equine Metabolic Syndrome (EMS), characterized by insulin resistance, fat deposition, and increased inflammation. Despite the growing prevalence of obesity in horses, knowledge of equine adipocytes and their metabolic functions remains limited. The main objective of the study was to develop and optimize a method for isolating equine adipocytes and to characterize their metabolic activity. Using slaughterhouse-derived horse visceral adipose tissue, we developed a protocol to isolate mature adipocytes. Metabolic activity of cells was assessed by examining their sensitivity to lipolytic factors: isoproterenol (0.001–10 µM), epinephrine (0.001–1 µM), and forskolin (0.001–1 µM)—and lipogenesis intensity after stimulation with insulin. We obtained mature equine adipocytes with diameters ranging from 50 to 160 µm. These cells demonstrated full metabolic functionality, responding to lipolytic factors such as isoproterenol (all doses: p &lt; 0.001), epinephrine (0.01 µM: p &lt; 0.05; 0.1–1 µM: p &lt; 0.0001), and forskolin (0.001 µM: p &lt; 0.0001). The adipocytes also responded to insulin from all tested species, with effects being dose- and time-dependent (after 2 h human insulin 10 nM, p &lt; 0.05; bovine 10, 100 nM p &lt; 0.05 and after 8 h all doses p &lt; 0.05). The presented method for isolating mature equine adipocytes is effective, yielding metabolically functional cells, which can serve as a valuable in vitro model for studying the effects of various factors on adipocyte function, contributing to a better understanding of equine adipose tissue dysfunction, particularly in the context of metabolic disorders.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106354"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of the use of peri-operative systemic ketamine in cats and dogs for analgesia","authors":"Francesca Wickstead ,&nbsp;Miguel Martinez","doi":"10.1016/j.tvjl.2025.106350","DOIUrl":"10.1016/j.tvjl.2025.106350","url":null,"abstract":"<div><h3>Objectives</h3><div>Ketamine is a non-competitive N-methyl-D-aspartate antagonist widely used in veterinary medicine for analgesia peri-operatively, in polytrauma and anecdotally for chronic pain. This systematic review aimed to provide an overview of the veterinary literature available on systemic ketamine administration for acute analgesia in dogs and cats. This will include, a discussion on ketamine’s impact on pain scores and rescue analgesia requirements and will correlate these with ketamine plasma concentrations and nociceptive threshold changes.</div></div><div><h3>Methods</h3><div>A literature search was conducted over three databases (GoogleScholar, PubMed and Veterinary Anaesthesia and Analgesia Journal) with definitive search terms and inclusion criteria between 1980 and 2024. Quality of evidence was assessed with the ‘Grading of Recommendations, Assessment, Development, and Evaluations’ and the Cochrane Collaboration Bias tool to assess study bias.</div></div><div><h3>Results</h3><div>Studies that met eligibility criteria included 11 dogs, three cats; 14 in total. These included prospective randomised controlled trials, crossover trials, case series and laboratory experiments. Quality of evidence was moderate, with low risk of bias, moderate-good study design, moderate indirectness due to predominantly soft tissue procedures studied and underpowered, small population studies.</div></div><div><h3>Conclusion</h3><div>Ketamine may influence pain scores &gt; 12 hours post operatively. Ketamine does not influence rescue analgesia requirements post-operatively. Plasma concentrations &gt; 200 ng ml⁻¹ correlate with nociceptive threshold changes. When doses are extrapolated from humans, plasma concentrations are lower than expected. Plasma concentrations quickly decline after cessation of intravenous infusion or bolus. Future studies with higher doses of ketamine in a variety of clinical procedures are needed to evaluate ketamine's analgesic effect peri-operatively.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106350"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the performance of VP1 expressed in baculovirus and Escherichia coli expressed from Senecavirus A in pig using an ELISA","authors":"Parin Watcharavongtip ,&nbsp;Patumporn Jermsutjarit ,&nbsp;Angkana Tantituvanont ,&nbsp;Dachrit Nilubol","doi":"10.1016/j.tvjl.2025.106346","DOIUrl":"10.1016/j.tvjl.2025.106346","url":null,"abstract":"<div><div>Senecavirus A (SVA) causes porcine idiopathic vesicular disease (PIVD), leading to economic losses in swine production. Rapid antibody detection is essential for monitoring herd immunity and controlling outbreaks. This study developed indirect ELISAs using SVA VP1 protein expressed in baculovirus and <em>Escherichia coli</em> (<em>E. coli</em>) systems. The optimized ELISAs showed high sensitivity (baculovirus: 100 %, <em>E. coli</em>: 96.67 %) and specificity (both 96.67 %) with a cut-off of 0.40. Both correlated strongly with virus neutralization assays (VNA) and showed no cross-reactivity with other porcine pathogens. The agreement with VNA was strong (κ = 0.839) for the baculovirus-based ELISA and moderate (κ = 0.731) for the <em>E. coli</em>-based ELISA. These findings suggest that SVA VP1 protein from both expression systems can serve as an alternative for ELISA-based serological diagnosis of SVA, aiding in herd immunity assessment and disease control.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106346"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance","authors":"Jiajia Yang ,&nbsp;Xuan Wang ,&nbsp;Ying Chen ,&nbsp;Ye He ,&nbsp;Ping Li ,&nbsp;Xin Wen ,&nbsp;Bi Wang","doi":"10.1016/j.tvjl.2025.106343","DOIUrl":"10.1016/j.tvjl.2025.106343","url":null,"abstract":"<div><div>Cecal coccidiosis is a severe and lethal parasitic disease affecting chickens, making the search for effective preventive agents free of contamination and drug resistance crucial for controlling this condition in poultry. Previous studies have demonstrated that the ChangQing compound has significant therapeutic effects against cecal coccidiosis; however, its potential as a preventive measure has yet to be evaluated. In this study, we established an experimental model for the prevention of cecal coccidiosis in chickens using the ChangQing compound for the first time. A comprehensive evaluation was performed on survival rates, relative weight gain, oocyst production, the anticoccidial index (ACI), immune parameters, parasitic tissue pathology, and microbial diversity in cecal contents. Results indicated that the ChangQing compound at a concentration of 5.0 g/L achieved an ACI of 178.10 in the Pre-Exposure Prophylaxis (PrEP) group and 173.12 in the Post-Exposure Prophylaxis (PEP) group. Compared to the positive control group, cecal lesions were reduced, and indices for the spleen, liver, and bursa of Fabricius increased in both experimental groups following ChangQing administration. Furthermore, levels of immune factors, IgA, IgG, and IgM significantly elevated. The abundance of beneficial bacteria, including <em>Lactobacillus</em>, <em>Bacteroides</em>, and <em>Alistipes</em>, increased in the 5.0 g/L ChangQing compound group, while potential pathogens like <em>Escherichia-Shigella</em>, <em>Enterococcus</em>, and <em>norank_f_Oscillospiraceae</em> were reduced. These findings offer critical data for coccidiosis prevention in chickens and lay a theoretical foundation for future research on the antiparasitic mechanisms of traditional Chinese medicine.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106343"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}