Veterinary journal最新文献

{"title":"The effects of breed and routes of administration on the plasma pharmacokinetics and faecal excretion of robenacoxib in goats","authors":"Zeynep Ozdemir Kutahya ,&nbsp;Cengiz Gokbulut ,&nbsp;Busra Aslan Akyol ,&nbsp;Oguzhan Yavuz ,&nbsp;Fazilet Sen ,&nbsp;Petek Piner Benli","doi":"10.1016/j.tvjl.2024.106200","DOIUrl":"10.1016/j.tvjl.2024.106200","url":null,"abstract":"<div><p>Robenacoxib (RX) is a non-steroidal anti-inflammatory drug (NSAID) of the coxib class. This study aimed to evaluate the plasma dispositions and faecal excretion profiles of RX in Alpine and Saanen goats following oral and subcutaneous routes. Two different goat breeds were allocated into two treatment groups concerning the breed. RX was administered subcutaneously to animals at a dose of 4 mg/kg b.w. Following a one-week washout period, RX was administered by oral route to the same animals at the same dose. Heparinized blood samples were collected from all animals before drug administration (0 h) and subsequently up to 24 h. Faecal samples were collected at various times between 8 h and 36 h. The concentrations of RX in plasma and faeces were determined by HPLC. The plasma half-life (T_1/2λz) of RX in Saanen goats (1.21 h) was significantly longer (<em>P</em> &lt; 0.017) than in Alpine goats (0.90 h) after subcutaneous administration. In both goat breeds, statistical differences were observed between subcutaneous and oral administration of RX for T_1/2λz, T_last, C_max, AUC_0-∞, and MRT_0-∞. Faecal C_max and T_max parameters following oral administrations were 0.92 µg/g and 0.85 µg/g at 30 h and at 24 h in Alpine and Saanen goats, respectively. The difference in plasma protein ratio between Alpine and Saanen goats may have affected the T_1/2λz of the drug. NSAIDs are among the drug groups frequently detected in aquatic and terrestrial ecosystems around the world and there are data on the effects of NSAID residues on wildlife and aquatic species. Therefore, revealing the excretion of NSAIDs, which are frequently used in the veterinary field, in faeces and urine should be considered for ecological sustainability.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106200"},"PeriodicalIF":2.3,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"European Network for Optimization of Veterinary Antimicrobial Therapy (ENOVAT) guidelines for antimicrobial use in canine acute diarrhoea","authors":"L.R. Jessen ,&nbsp;M. Werner ,&nbsp;D. Singleton ,&nbsp;C. Prior ,&nbsp;F. Foroutan ,&nbsp;A.A. Ferran ,&nbsp;C. Arenas ,&nbsp;C. R. Bjørnvad ,&nbsp;E. Lavy ,&nbsp;F. Allerton ,&nbsp;K. Allensbach ,&nbsp;L. Guardabassi ,&nbsp;S. Unterer ,&nbsp;T. Bodnárová ,&nbsp;U. Windahl ,&nbsp;M.L. Brennan ,&nbsp;J.S. Weese ,&nbsp;K. Scahill ,&nbsp;ESCMID Study Group for Veterinary Microbiology (ESGVM), European Network for Optimization of Antimicrobial Therapy (ENOVAT)","doi":"10.1016/j.tvjl.2024.106208","DOIUrl":"10.1016/j.tvjl.2024.106208","url":null,"abstract":"<div><p>Acute diarrhoea is a common presentation in dogs, and a common reason for antimicrobial prescription and nutraceutical use. This evidence-based guideline provides recommendations for antimicrobial and probiotic treatment of canine acute diarrhoea (CAD). A multidisciplinary panel developed the recommendations by adhering to the Grading of Recommendations Assessment, Development and Evaluation (GRADE) framework. The opinions of stakeholders (general veterinary practitioners and dog owners) were collected and incorporated to ensure the applicability of this guideline. Four strong recommendations informed by high certainty evidence, and three conditional recommendations informed by very low or low certainty evidence, were drafted by the panel, along with an ungraded section on diagnostic work-up of dogs with acute diarrhoea. The ENOVAT guidelines initiative encourages national or regional guideline makers to use the evidence presented in this document, and the supporting systematic review, to draft national or local guidance documents.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106208"},"PeriodicalIF":2.3,"publicationDate":"2024-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1090023324001473/pdfft?md5=df746a0a00f74f15d6e74d234b9b3127&pid=1-s2.0-S1090023324001473-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141793624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a virtual reality simulator for training canine endotracheal intubation technique and evaluation of the educational impacts","authors":"A. Yamauchi ,&nbsp;R. Oshita ,&nbsp;A. Kudo ,&nbsp;M. Umezawa ,&nbsp;R. Shimizu ,&nbsp;S. Kamo ,&nbsp;Y. Fujita ,&nbsp;S. Takagi","doi":"10.1016/j.tvjl.2024.106203","DOIUrl":"10.1016/j.tvjl.2024.106203","url":null,"abstract":"<div><p>Virtual reality (VR)-based training has shown some benefits in medical education, supporting skill acquisition, and helping reduce anxiety in real-world settings. However, the use of VR simulators in veterinary education remains limited. This study aimed to introduce a VR simulator to support veterinarian training in canine anaesthesia induction and endotracheal intubation. This study involved a group that learned solely with instructional videos (video group), and one that learned concurrently with the video and VR simulator (VR group). Third- and fourth-year veterinary students were included and underwent a descriptive test on canine endotracheal intubation. Canine endotracheal intubation success rates were compared between the video (n = 364) and VR (n = 60) groups of fifth-year students. A survey on the VR usability was conducted (n=91). The median descriptive test scores improved in the VR (63.3/100) vs the video group (51.5/100). The canine intubation success rates were comparable in the VR and video groups at 84.3 % and 77.4 %, respectively. A total of 90.1 % of the surveyed students rated the ease of use of the simulator highly. Overall, VR simulators were well-received, suggesting benefits in new skill retention. Further studies are required to evaluate the extent of skill improvement through VR-based training, compared to conventional methods, and to assess its impact on student motivation. Evaluating the long-term effects of VR-based training on skill development and retention will also provide a deeper understanding of its educational benefits.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106203"},"PeriodicalIF":2.3,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic study of local and systemic gentamicin concentrations after subcutaneous implantation of a gentamicin-impregnated collagen sponge in dogs","authors":"T. Bhawono Dadi ,&nbsp;N. Devriendt ,&nbsp;M. Cherlet ,&nbsp;M. Devreese ,&nbsp;I. Polis ,&nbsp;H. de Rooster","doi":"10.1016/j.tvjl.2024.106201","DOIUrl":"10.1016/j.tvjl.2024.106201","url":null,"abstract":"<div><p>This study evaluated the pharmacokinetics of commercial gentamicin-impregnated collagen sponges (GICS) applied subcutaneously in dogs. In six healthy beagles, an 11 ×6 cm subcutaneous pocket was created, a folded 10×10 cm GICS was inserted, and saline was injected to mimic a seroma. Wound fluid samples were aspirated, and the gentamicin concentration was determined. Simultaneously, blood samples were collected to evaluate the corresponding systemic gentamicin concentration. All samples were collected before and 0.5, 1, 1.5, 2, 3, 4, 6, 8, 12, 18, 24, 36, 48, 72, 96, 120, and 168 hours after GICS placement. The local C_max of gentamicin was reached after 0.5 hours (range, 0.5–1.0 hours) post-implantation in 5/6 dogs at a median concentration of 2053.3 µg/mL (range, 918.0–2791.9 µg/mL). Whitin 24 hours, the local concentration dropped below the MIC for <em>Staphylococcus sp</em>. (4 µg/mL) in 5/6 dogs. Plasma C_max was achieved at a median of 1.2 hours post-implantation (range, 1.0–2.0 hours) and reached a median concentration of 10.3 µg/mL (range, 8.8–18.03 µg/mL). After 6 hours, the gentamicin concentration in the plasma was below 4 µg/mL in all dogs. The GICS provided a high local concentration of gentamicin in a short time with a local Cmax:MIC ratio of 513:1, largely sufficient to eliminate susceptible bacteria, including methicillin-resistant <em>Staphylococcus pseudintermedius</em> (MRSP) and <em>Pseudomonas sp</em>., in a clinical setting. The repeated administration of saline in the present study seemed to have induced a quicker gentamicin release from the GICS than described in previous studies that typically dealt with \"drier\" wounds.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106201"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of climatic changes on respiratory health in a teaching herd of outdoor-housed horses","authors":"Stefano Strano Calomeno ,&nbsp;Thasla de Freitas Santi ,&nbsp;Bianca Barbosa ,&nbsp;Saulo Henrique Weber ,&nbsp;Tiago Marcelo Oliveira ,&nbsp;Gisele Fabrino Machado ,&nbsp;Pedro Vicente Michelotto","doi":"10.1016/j.tvjl.2024.106198","DOIUrl":"10.1016/j.tvjl.2024.106198","url":null,"abstract":"<div><p>Horses maintained outdoors may experience a lower-allergenic environment compared to their stabled counterparts. This study hypothesizes that climatic changes in southern Brazil can influence respiratory status. To test this hypothesis, we evaluated the lower airways of 17 horses from a teaching herd in southern Brazil, maintained outdoors, during winter, spring, and summer. Except for one mare with a history of severe asthma, all horses were considered healthy and underwent a physical examination. Airway endoscopic evaluation included scoring for tracheal mucus (0−5) and bronchial septum thickness (1−5). Bronchoalveolar lavage fluid (BALF) was collected at three time points, while bronchial septum biopsies were performed during spring and summer for airway epithelial investigation. Data analysis involved repeated measures ANOVA and Wilcoxon tests (p &lt; 0.05). Tracheal mucus score and septal thickness did not differ across investigation periods. In BALF cytology, the mean percentage of neutrophils was higher in spring than summer (7.9 ± 13.4 % vs. 4.5 ± 11.7 %, <em>P</em> = 0.037), and eosinophil count was higher in winter than spring (0.64 ± 1.29 % vs. 0.03 ± 0.13 %, <em>P</em> = 0.034) and summer (0.64 ± 1.29 % vs. 0.14 ± 0.60 %, <em>P</em> = 0.023). Histopathological observations showed no differences between time points, and no correlations were observed with BALF analyses (<em>P</em> &gt; 0.05). This study demonstrates that, even in an outdoor environment, horses' airways exhibit cytological modifications associated with different seasons, indicating a need for deeper investigation; endobronchial biopsy did not contribute to the clinical diagnosis.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106198"},"PeriodicalIF":2.3,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myostatin serum levels depends on age and diet in athletic and no athletic dogs","authors":"Sandra Bendig ,&nbsp;Pablo Jesús Marín-García ,&nbsp;Ana Lesta ,&nbsp;Juan José Ramos ,&nbsp;Guillem Ruvira ,&nbsp;Lola Llobat","doi":"10.1016/j.tvjl.2024.106207","DOIUrl":"10.1016/j.tvjl.2024.106207","url":null,"abstract":"<div><p>Myostatin is a growth factor related to muscular mass atrophy via mTOR pathway inhibition. Mutations in this gene have been correlated with high muscular mass development in different species of mammals, including human and dogs. Different studies have shown that sport practice increases myostatin gene expression. Some of them were conducted in canine breeds selected for different sport practices, including mushing sports. In this study, body weight, muscular mass, and serum levels of myostatin were analysed in different canine breeds, selected, and not selected for sprint and middle-distance racing, and the effect on epidemiological factors was evaluated. Sex, reproductive status, and canine breed affects body weight and muscular mass, being higher in males, and in sled canine breed. Age has an effect in body weight and myostatin serum levels, being lower in elder dogs. Sport practice and type of diet had an effect in muscular mass development but not in myostatin serum levels. Results showed a high positive correlation between muscular mass and body weight but not with myostatin levels. These results suggest that independent-myostatin mechanisms of mTOR pathway regulation could be related to muscular mass development in dogs.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106207"},"PeriodicalIF":2.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1090023324001461/pdfft?md5=1ec684a3862062cf7d98fee3482d4c4f&pid=1-s2.0-S1090023324001461-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of different classifications of hypocalcemia with quantity and quality of colostrum, milk production, and health of Holstein dams and their calves","authors":"H.R. Sohrabi ,&nbsp;T. Amirabadi Farahani ,&nbsp;S. Karimi-Dehkordi ,&nbsp;N.E. Farsuni","doi":"10.1016/j.tvjl.2024.106205","DOIUrl":"10.1016/j.tvjl.2024.106205","url":null,"abstract":"&lt;div&gt;&lt;p&gt;The aim of the present study was to assess the effect of serum calcium (Ca) status in the early postpartum period on the quantity and quality of colostrum, milk production, and the health of Holstein dams and their calves. One hundred multiparous Holstein cows were enrolled in this study. To determine serum Ca status, blood samples were taken at 1 and 4 days in milk (DIM). Cows were categorized into 1 of 4 groups based on their serum Ca concentrations: normocalcemic (CON; Ca &gt;1.87 at 1 DIM and &gt;2.10 mmol/L at 4 DIM, n = 36), transient SCH (TSCH; Ca ≤1.87 at 1 DIM and &gt;2.10 mmol/L at 4 DIM, n = 14), persistent SCH (PSCH; Ca ≤1.87 at 1 DIM and ≤2.10 mmol/L at 4 DIM, n = 15), or delayed SCH (DSCH; Ca &gt;1.87 at 1 DIM and ≤2.10 mmol/L at 4 DIM, n = 35). The ROC curve was utilized to determine the optimal cut-off value for serum total Ca, maximizing both sensitivity and specificity in predicting metritis. Metabolic disorders such as dystocia, retained placenta, and culling within 60 DIM were not affected by serum Ca status in the early postpartum period (&lt;em&gt;P&lt;/em&gt; &gt; 0.10). However, the incidences of metritis and endometritis were different based on the serum Ca status of the cows. The PSCH cows had a higher incidence of metritis than the CON cows (&lt;em&gt;P&lt;/em&gt; = 0.05). Also, the DSCH cows tended to have an increased risk of metritis than the CON cows (&lt;em&gt;P&lt;/em&gt; = 0.09). However, metritis incidence in the TSCH and CON cows was similar (&lt;em&gt;P&lt;/em&gt; = 0.83). Cows with PSCH (&lt;em&gt;P&lt;/em&gt; = 0.07) and DSCH (&lt;em&gt;P&lt;/em&gt; = 0.10) tended to be at a higher risk for endometritis than the CON cows. But, the incidence of endometritis in the TSCH and CON cows was not different (&lt;em&gt;P&lt;/em&gt; = 0.50). The TSCH cows had higher milk yield than the PSCH and DSCH cows (&lt;em&gt;P&lt;/em&gt; = 0.05). However, milk yield was not different between the TSCH and CON cows and the PSCH and DSCH and CON cows. The serum Ca status of cows had no effect on colostrum volume and its quality, age and weight of weaning, and average daily gain of calves (&lt;em&gt;P&lt;/em&gt; &gt; 0.10). The proportion of calves experiencing failure of passive transfer (FPT) was greater in the PSCH group than in the CON group (&lt;em&gt;P&lt;/em&gt; = 0.03). Also, the FPT tended to be higher in the DSCH calves compared to the CON calves (&lt;em&gt;P&lt;/em&gt; = 0.10). However, the FPT in the TSCH and CON calves was not different (&lt;em&gt;P&lt;/em&gt; = 0.83). The incidence of diarrhea tended to be higher in the PSCH and DSCH calves compared to the CON calves (&lt;em&gt;P&lt;/em&gt; = 0.10). However, diarrhea incidence between the TSCH and CON calves was similar (&lt;em&gt;P&lt;/em&gt; = 0.97). Overall, the findings of this study suggest that cows with TSCH showed better adaptation to lactation, experienced a lower occurrence of diseases and produced higher milk compared to PSCH and DSCH cows. Furthermore, despite the similar quantity and quality of colostrum across all groups, the PSCH and DSCH groups displayed a greater proportion of calves experiencing FPT and diar","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106205"},"PeriodicalIF":2.3,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141767440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking perspectives on surgical sterilization in dogs and cats: A comprehensive study among Thai veterinary professionals","authors":"S. Leelakajornkit ,&nbsp;P. Kamdee ,&nbsp;S. Ponglowhapan","doi":"10.1016/j.tvjl.2024.106206","DOIUrl":"10.1016/j.tvjl.2024.106206","url":null,"abstract":"<div><p>Gaining insights into the opinions and attitudes of veterinarians is essential for enhancing the sterilization rate, given their influential role in guiding pet owners. This study investigated the attitudes and practices of Thai veterinarians concerning surgical sterilization in dogs and cats. The survey was conducted from August 2022 to December 2022 and distributed through online and on-site questionnaires across all regions of Thailand. Seven hundred twenty-four veterinarians participated. A strong inclination toward sterilization for population control with a notable preference for female animals was revealed. The factors related to the necessity of sterilization in the opinion of Thai veterinarians were gender, age of veterinarians, and animal sex (P&lt;0.05). Male veterinarians tended to assign less importance to sterilization, especially for male dogs (OR = 0.824), compared to their female counterparts. Older veterinarians tended to prioritize sterilization less than younger ones (OR = 0.985). Regarding animal’s sex, a preference for sterilizing females over males in both dogs and cats (OR = 0.291) was observed. Encouraging sterilization should be promoted equally among veterinarians of both genders, with particular emphasis on older male veterinary practitioners. Most veterinarians recommended sterilization between 4 and 6 months for female cats and 7–10 months for male cats, male dogs and female dogs. Overall, mean agreement scores for prepubertal gonadectomy were significantly higher (P&lt;0.05) for female dogs and cats, and the only significant factor associated with these scores was the animal's sex. Veterinarians expressed more disagreement with prepubertal sterilization in males compared to females, with an OR of 0.33. There were some concerns in prepubertal sterilization such as growth and development problems, anesthesia risks, and urinary tract disorders. These insights observed in Thailand hold potential implications for other countries facing similar challenges in effectively managing pet overpopulation.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106206"},"PeriodicalIF":2.3,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141761232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble expression and immunogenicity analysis of capsid proteins of porcine circoviruses types 2, 3, and 4","authors":"Huimin Zhang ,&nbsp;Xue Li ,&nbsp;Xinru Lv ,&nbsp;Yaqi Han ,&nbsp;Jiawei Zheng ,&nbsp;Linzhu Ren","doi":"10.1016/j.tvjl.2024.106199","DOIUrl":"10.1016/j.tvjl.2024.106199","url":null,"abstract":"<div><p>Porcine circoviruses (PCVs) contain four types: PCV1, PCV2, PCV3, and PCV4, all of which can infect pigs. Among them, PCV1 is non-pathogenic, and PCV2 can cause porcine circovirus diseases (PCVD) or porcine circovirus-associated diseases (PCVAD). Although the pathogenicity of PCV3 and PCV4 is still controversial, increasing evidence shows that PCV3 and PCV4 can cause PCV-related disease. However, mixed infection of PCV2, PCV3, and PCV4 with other pathogens often occurs in large-scale pig breeding, bringing severe economic losses to the global pig industry. In this study, the soluble recombinant proteins of PCV2, PCV3, and PCV4 Cap were expressed by the prokaryotic expression system and biotinylated to combine with the Streptavidin magnetic beads, followed by immunogenicity evaluation of the recombinant proteins. Furthermore, we also assessed the efficacy and immunogenicity of trivalent recombinant proteins conjugated with different adjuvants in mice. The results showed that the highly effective anti-PCV serum was successfully prepared, and the recombinant proteins conjugated with different adjuvants produced various degrees of humoral and cellular immunity in mice. Three recombinant proteins are effective immunogens, and the trivalent proteins coupled with the aluminum adjuvant or GM-CSF-CpG for two-dose immunization can stimulate prominent humoral and cellular immunity against PCVs <em>in vivo</em>. The soluble recombinant proteins are the most promising candidate for developing a trivalent vaccine against PCVs (PCV2, PCV3, and PCV4) infection simultaneously.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"307 ","pages":"Article 106199"},"PeriodicalIF":2.3,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141749163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective study of successful autologous dendritic cell therapy in dogs with splenic stage II hemangiosarcoma","authors":"V. Spiller ,&nbsp;M. Vetter ,&nbsp;C. Dettmer-Richardt ,&nbsp;T. Grammel","doi":"10.1016/j.tvjl.2024.106196","DOIUrl":"10.1016/j.tvjl.2024.106196","url":null,"abstract":"<div><p>Hemangiosarcoma is an aggressive tumour that most frequently occurs in larger, middle-aged dogs of certain breeds. The spleen is the most commonly affected organ. The aim of this prospective therapy study was to evaluate the clinical effect of autologous, monocyte-derived dendritic cell (DC) therapy in canine hemangiosarcoma stage II after splenectomy. Dogs (<em>n</em>=452) diagnosed with splenic hemangiosarcoma that underwent splenectomy were enrolled. Of these, 42 dogs with stage II entered the DC therapy study. The median survival time for the total group of 42 dogs was 203 days. The median survival for the group (<em>n</em>=34) that received the full DC therapy (≥3 vaccines) was 256 days, with a 29 % one-year survival rate and a hazard ratio of 0.30, adjusted to age and bodyweight (<em>P</em>=0.010). We further observed a significant increase in DC yield after each application and demonstrated that DC yield at the beginning of treatment is significantly related to patient survival. While further evidence is needed, we conclude that autologous, monocyte-derived DC therapy is a viable alternative to standard treatment methods of canine splenic stage II hemangiosarcoma.</p></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"306 ","pages":"Article 106196"},"PeriodicalIF":2.3,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}