Veterinary journal最新文献

{"title":"Liraglutide as a Novel Therapeutic for Overweight in Canines: A Clinical Study.","authors":"Burak DiK, Durmuş Hatipoglu, Oguzhan Kahraman, Tugba Melike Parlak, Zekeriya Safa Inanc, Metin Celik, Samed Damar","doi":"10.1016/j.tvjl.2025.106376","DOIUrl":"https://doi.org/10.1016/j.tvjl.2025.106376","url":null,"abstract":"<p><strong>Background: </strong>The overweight/obesity in dogs is defined as a condition of abnormal fat accumulation or, beyond that, a multifactorial condition involving excessive fat accumulation along with different factors (insufficient physical activity, genetics (breed), metabolism, the microbiome, etc.). Preclinical obesity conditions increases the risk of progressing to clinical obesity and developing obesity-associated diseases such as type 2 diabetes, cardiovascular disease, certain cancers, and mental disorders.</p><p><strong>Objectives: </strong>This study aimed to determine the weight-loss effects of liraglutide in dogs that do not engage in sufficient exercise, are fed table scraps, and develop obesity owing to aging.</p><p><strong>Animals: </strong>In this study, 21 senior Golden Retriever dogs were divided into three equal groups.</p><p><strong>Methods: </strong>Group 1 was considered healthy and consisted of dogs within the range of ideal body live weight and body condition scores, and fed dry commercial dog food at the amount of their daily metabolizable energy requirements. Group 2 included seven senior dogs with obesity fed commercial dry food adjusted for their daily metabolizable energy requirements. Group 3 comprised seven senior dogs with obesity fed commercial dry food adjusted for their daily energy requirements and concurrently treated with subcutaneous liraglutide at a dose of 1.2mg/dog. The trial lasted for 40 days.</p><p><strong>Results: </strong>Liraglutide treatment resulted in a decrease in body condition score (BCS) and body weight by day 40, although the reduction in body weight (13.27%) did not reach statistical significance. Liraglutide significantly lowered cholesterol and triglyceride levels, and appetite tests revealed a marked suppression of food intake over consecutive days in treated dogs.</p><p><strong>Conclusions and clinical importance: </strong>Liraglutide treatment may offer a viable option for treating obesity in dogs, and could potentially be used as a new anti-obesity drug in canines. Future long-term and detailed trials of liraglutide in dogs with obesity could facilitate its effective use in the field.</p>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":" ","pages":"106376"},"PeriodicalIF":2.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and Epidemiology of Bovine Coronavirus in Cattle in Kazakhstan.","authors":"Yuliya V Perfilyeva, Andrey V Zhigailov, Angelina A Malysheva, Alena S Cherusheva, Karina R Ivanova, Zhanna A Berdygulova, Akerke O Bissenbay, Saltanat A Kuatbekova, Zhaniya M Dosmagambet, Anzhelika V Lushova, Sofiya A Kan, Artyom V Kuligin, Moldir M Kuatbek, Akzhigit S Mashzhan, Nurshat Abdolla, Dinara A Naizabayeva, Anna S Nizkorodova, Perizat B Akshalova, Aida M Abdybekova, Aralbek S Rsaliyev, Yergali O Abduraimov, Seidigapbar M Mamadaliyev, Yuriy A Skiba, Yekaterina O Ostapchuk","doi":"10.1016/j.tvjl.2025.106371","DOIUrl":"https://doi.org/10.1016/j.tvjl.2025.106371","url":null,"abstract":"<p><p>Bovine coronavirus (BCoV) causes gastrointestinal and respiratory diseases in cattle, but its prevalence in Kazakhstan remains unknown. This nationwide cross-sectional study aimed to assess BCoV prevalence in cattle and to investigate its epidemiological characteristics. Between April and August 2024, serum, nasal and rectal swab samples were collected from 2,237 clinically healthy cattle across 390 farms in 17 oblasts of Kazakhstan. None of the farm owners reported vaccinating against BCoV. Serum samples were tested for BCoV-specific antibodies using a commercially available ELISA, while nested RT-PCR assays were performed on pooled nasal and rectal swabs to detect BCoV RNA. Sequencing and phylogenetic analysis were performed following RT-PCR testing. A univariate GEE model was used to assess the risk factors and a multivariate model was employed to refine the discovered associations. The animal-level seroprevalence of BCoV was 88.2% (95% CI: 84.3%-92.0%), while the herd-level seroprevalence was 89.6% (95% CI: 85.4%-92.9%). The eastern region of Kazakhstan had the lowest seroprevalence compared to other regions. Seropositivity was not associated with age, sex or breed, but correlated with the proportion of cattle in backyards, cattle density and farm size. BCoV shedding was detected in 2.4% (95% CI: 1.4 5% - 3.4%) of sampled animals and in 7.8% (95%CI: 5.3% - 11.0%) of cattle operations across 12 oblasts. Phylogenetic analysis of the complete hemagglutinin/esterase and spike genes revealed that the Kazakhstani BCoV strains belonged to the GIIa group. This first report on BCoV in Kazakhstan confirms its endemic presence, highlighting the need for a national control program.</p>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":" ","pages":"106371"},"PeriodicalIF":2.3,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiotherapy treatment of musculo-tendinous disorders of the canine shoulder: A clinical study","authors":"Ilaria Arena ,&nbsp;Simona Valentini ,&nbsp;Linda Nundini ,&nbsp;Thomas Dalmonte ,&nbsp;Giuseppe Spinella","doi":"10.1016/j.tvjl.2025.106379","DOIUrl":"10.1016/j.tvjl.2025.106379","url":null,"abstract":"<div><div>In veterinary literature, pathologies affecting the shoulder region in dogs are increasingly described, particularly in dogs that practice high-impact sporting activities. Physiotherapy may represent a complementary and supportive treatment to surgery and conservative therapy; however, the supporting scientific evidence is still limited. This study aims to evaluate a multimodal physiotherapy protocol in 25 dogs suffering from shoulder pathologies, examining time and number of sessions required to gain lameness recovery and trying to identify which variables (age, sex, weight, degree of lameness, presence of osteoarthritis, modalities) are able to positively or negatively influence recovery times. Patients received treatment consisting mainly of laser therapy and ultrasound combined with muscle strengthening exercises, hydrotherapy and manual techniques. Data were submitted to a descriptive and statistical analysis to highlight the recovery times and the most important variables. The results showed a mean clinical recovery time of 50 ± 35 days (median: 35 days) with a number of sessions equal to 10 ± 3 sessions (median: 10 sessions). Age, the presence of osteoarthritis, association between Laser and ultrasound therapy and the initial severity of the lameness significantly influenced recovery times. The study highlights the importance of a multimodal physiotherapeutic approach and underlines the need to carry out further large-scale research to standardize treatments and evaluate the impact on return to sporting activity.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106379"},"PeriodicalIF":2.3,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-based analysis for the identification of candidate genes associated with skin-photosensitization tolerance in sheep","authors":"Slim Ben Jemaa ,&nbsp;Alberto Cesarani ,&nbsp;Elena Ciani ,&nbsp;Salvatore Mastrangelo","doi":"10.1016/j.tvjl.2025.106380","DOIUrl":"10.1016/j.tvjl.2025.106380","url":null,"abstract":"<div><div>In ruminant livestock, skin photosensitization, caused by the ingestion of toxic plants, is relatively common and affects animal production. In this study, genotyping data from the Illumina OvineSNP50 BeadChip from two Italian local sheep breeds (Leccese and Altamurana) were used to identify putative genomic regions associated with response to skin photosensitization. We identified four genomic regions harbouring several candidate genes related to dermatitis, immune response, and coat color, that could be potentially involved in modulating photosensitization in sheep. These findings enhance our understanding of the genetic mechanisms underlying skin photosensitization in sheep and provide valuable insights into livestock adaptation to local environmental pressure.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106380"},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preoperative and postoperative joint motion in chihuahuas with Grade III medial patellar luxation: A kinematic and goniometric analysis","authors":"Kannika Chayatup ,&nbsp;Chalika Wangdee ,&nbsp;Kumpanart Soontornvipart","doi":"10.1016/j.tvjl.2025.106369","DOIUrl":"10.1016/j.tvjl.2025.106369","url":null,"abstract":"<div><div>Medial patellar luxation (MPL) is a common orthopaedic disorder in dogs, resulting in stifle instability and altered pelvic limb biomechanics. While its effects on stifle function have been extensively documented, the influence on adjacent joints and the course of biomechanical recovery following surgical correction remain poorly characterized. This study aimed to evaluate active (AROM) and passive (PROM) range of motion in the stifle, hip, and tarsal joints of Chihuahuas with Grade III MPL using two-dimensional kinematic and goniometric analyses. Seventeen client-owned Chihuahuas were enrolled, comprising ten MPL-affected dogs and seven clinically healthy controls. MPL dogs underwent surgical correction, and joint motion assessments were performed preoperatively and at 2, 4, 6, and 8 weeks postoperatively. Significant reductions in stifle AROM and PROM, particularly in extension, were observed in the MPL group compared to controls, indicating functional impairment. Compensatory increases in hip and tarsal AROM were also detected preoperatively, suggesting biomechanical adaptation. Following surgery, stifle AROM progressively improved and approached control values by week 8, whereas stifle PROM remained consistently reduced. Temporal changes in hip and tarsal AROM suggested early compensatory adaptations, which gradually resolved as stifle function improved. However, persistent stifle PROM limitations underscore the need for early, targeted rehabilitation. A multimodal strategy addressing both primary joint dysfunction and compensatory changes is essential to optimize recovery. Baseline joint motion data may serve as valuable benchmarks for evaluating surgical outcomes and guiding individualized postoperative care.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106369"},"PeriodicalIF":2.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial dispensing for common conditions in dogs and cats at a large veterinary practice network, 2023","authors":"J.Scott Weese ,&nbsp;Morgan E. Taylor-Rakocevic ,&nbsp;Kseniya Topdjian ,&nbsp;Ian Battersby","doi":"10.1016/j.tvjl.2025.106374","DOIUrl":"10.1016/j.tvjl.2025.106374","url":null,"abstract":"<div><div>Understanding antimicrobial use is a core component of antimicrobial stewardship. This study aimed to assess antimicrobial dispensing for common clinical conditions in dogs and cats presented to veterinary clinics belonging to a large clinic network in the USA. Antimicrobials were prescribed for 831,017 patient visits, to 702,576 (85 %) dogs and 128,441 (15 %) cats. Cefpodoxime (n = 203,145, 29 %), amoxicillin-clavulanate (154,779, 22 %) and metronidazole (150,830, 21 %) were the most commonly dispensed antimicrobials in dogs, while cefovecin (55,579, 43 %) and amoxicillin-clavulanate (44,857, 35 %) predominated in cats. In dogs, drugs classified by the WHO MIA List as highest priority critically important (HPCIA) accounted for 39 % of drugs dispensed, while those classified as highly important (HIA) accounted for 61 %. In cats, HPCIA drugs accounted for 46 % of drugs dispensed while HIA drugs accounted for 54 %. Consistency of drug selection with selected treatment guidelines was 76 % (30,562/40,375) for dogs and 57 % (12,810/22,644) for cats. There were regional differences in drug selection patterns for all of the 10 most common diseases, for both dogs and cats. While no single metric or data source provides a full understanding of antimicrobial use, these data provide the foundation for assessment of antimicrobial use practices and provide insight and baseline data for development of interventions to improve antimicrobial use practices.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106374"},"PeriodicalIF":2.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective evaluation of the diagnosis of acetabular fracture in cattle and the outcome after conservative treatment","authors":"Robin Näf ,&nbsp;David Devaux ,&nbsp;Andrea Bischofberger ,&nbsp;Karl Nuss","doi":"10.1016/j.tvjl.2025.106375","DOIUrl":"10.1016/j.tvjl.2025.106375","url":null,"abstract":"<div><div>Acetabular fractures are rare in cattle, and their prognosis has generally been considered unfavourable. The goal of this retrospective study was to determine the diagnosis, treatment, and outcome in 36 cattle with 37 acetabular fractures presented to the Unit of Ruminant Surgery, University of Zurich Veterinary Hospital, from January 2016 to November 2024. All cattle were female and mostly young (13 months, median age; range, 9 – 92 months). The median lameness score was 3/5 (range 1 – 5/5), and all cattle could stand. A diagnosis of acetabular fracture was based on clinical and radiographic findings. Adjunctive ultrasonography was used in 21 cases. Simple fractures were the most common and occurred in 25 cattle; one heifer had bilateral simple acetabular fractures with ischial involvement. Fractures with 1–2 additional fragments occurred in four cattle, and seven animals had comminuted fractures. Treatment consisted of stall rest for 3 months. Based on owner information, most cattle (31/36, 86.0 %) were sound after 3 months, and 34/36 (94.4 %) were free of lameness 6 months after admission. By the end of November 2024, most cattle (27/36) had calved at least once since admission to our clinic, and 24/25 cows (available information) had unassisted calvings. Twenty-one of the 36 cows were still productive at the end of the study. Based on our findings, the prognosis of acetabular fractures in cattle is favourable. Conservative treatment consisting of stall rest and pain management is straightforward, providing a good long-term outcome.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106375"},"PeriodicalIF":2.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Static respiratory compliance in anaesthetised and intubated brachycephalic dogs with and without brachycephalic obstructive airway syndrome","authors":"Matilda Fitzmaurice ,&nbsp;Chiara Adami ,&nbsp;Jane Ladlow ,&nbsp;Francesca Tomlinson ,&nbsp;Claudia Gittel","doi":"10.1016/j.tvjl.2025.106372","DOIUrl":"10.1016/j.tvjl.2025.106372","url":null,"abstract":"<div><div>The impact of brachycephalic obstructive airway syndrome in dogs (BOAS) on respiratory mechanics is unclear and may affect the choice of ventilation strategies during anaesthesia. This prospective study included 56 client-owned brachycephalic dogs, allocated to be BOAS (<em>n</em> = 26) or non-BOAS dogs (<em>n</em> = 30) based on functional grading. All dogs were anaesthetised using a standardised anaesthetic protocol. Pressure-controlled ventilation was initiated around 30 min post-induction, maintaining peak inspiratory pressure at 7–12 cm H₂O. Static respiratory compliance (Cstat) was recorded at predetermined time points in sternal, right and left lateral recumbency. Thorax dimensions were assessed with a tape measure. Body surface area (BSA) was calculated and the ratio Cstat/BSA used as the main outcome variable. Comparison of means/medians, analysis of proportions, the Spearman correlation coefficient and both logistic and linear regression were used for data analysis. P &lt; 0.05 was considered statistically significant. Non-BOAS dogs showed significantly higher Cstat/BSA compared to BOAS dogs in sternal (41.6 (31.1–51.8) vs. 32.9 (24.4 – 39.2), respectively, P = 0.028), right lateral (36.2 (25.7 – 46.4) vs. 27.0 (22.7 – 35.6); P = 0.026) and left lateral (33.6 (22.6 – 45.5) vs. 24.6 (18.4 – 32.2); P = 0.020) recumbencies. For all dogs, the Cstat/BSA ratio was higher in sternal compared to lateral recumbencies. BOAS dogs had a significantly shorter distance between thoracic inlet and last rib compared to non-BOAS dogs (20 ± 4 vs. 23 ± 6 cm, respectively; P = 0.043). Reduced respiratory compliance in BOAS-affected dogs should be considered during mechanical ventilation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106372"},"PeriodicalIF":2.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and classification of tooth resorption in small-breed dogs: A retrospective study using full-mouth intraoral radiographs","authors":"Jinhee Bae,&nbsp;Se Eun Kim,&nbsp;Kangmoon Seo","doi":"10.1016/j.tvjl.2025.106377","DOIUrl":"10.1016/j.tvjl.2025.106377","url":null,"abstract":"<div><div>Tooth resorption (TR) is a clinically significant dental condition in dogs; however, its classification and prevalence, particularly in small breeds, remain understudied despite its impact on quality of life. This retrospective study aimed to assess and classify the types of TR in small-breed dogs and investigate associations between TR and factors such as sex, skull type, body weight, number of missing teeth, TR type, breed, and age. Dental radiographs of 248 dogs weighing less than 15 kg, examined over a three-year period, were included in this study. Statistical analyses were performed to investigate the associations between the examined variables and TR. The most common type of resorption was external replacement resorption (ERR), followed by external inflammatory resorption (EIR). Significant associations were found among age, number of missing teeth, and total TR (<em>p</em> &lt; 0.01). In addition, the prevalence of ERR and EIR increased with age (<em>p</em> &lt; 0.01). The rate of TR was significantly higher in premolars than in incisors, canines, and molars (<em>p</em> &lt; 0.001). Maltese dogs showed a significantly higher prevalence of TR (<em>p</em> &lt; 0.05), particularly ERR (<em>p</em> &lt; 0.05), than other breeds. The TR rate increased significantly in dogs aged 9–11 years, 12–15 years, and over 15 years. Given the high prevalence of TR in older small-breed dogs, particularly in premolars and breeds such as Maltese, clinicians should exercise caution during tooth extraction. Thorough radiographic evaluation is recommended to accurately assess the extent of resorption, especially in older dogs and predisposed breeds.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106377"},"PeriodicalIF":2.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of circulating microRNAs in plasma from horses with non-strangulating intestinal infarction and idiopathic peritonitis","authors":"Katrine Toft ,&nbsp;Maibritt Mardahl ,&nbsp;Ylva Hedberg-Alm ,&nbsp;Karin Anlén ,&nbsp;Eva Tydén ,&nbsp;Martin K. Nielsen ,&nbsp;Marie Louise Honoré ,&nbsp;Bastian Fromm ,&nbsp;Lise N. Nielsen ,&nbsp;Peter Nejsum ,&nbsp;Stig Milan Thamsborg ,&nbsp;Susanna Cirera ,&nbsp;Tina Holberg Pihl","doi":"10.1016/j.tvjl.2025.106378","DOIUrl":"10.1016/j.tvjl.2025.106378","url":null,"abstract":"<div><div>Non-strangulating intestinal infarctions (NSII) associated with <em>Strongylus vulgaris</em> infection and idiopathic peritonitis (IP) share similar clinical presentation but require different treatment approaches. Horses with NSII need surgical intervention, while idiopathic peritonitis cases can be successfully treated with antimicrobials. A correct diagnosis is thus crucial, but because the two diseases overlap in clinicopathological features, differentiation is difficult in clinical practice. MicroRNAs (miRNAs) are non-coding RNAs that exhibit measurable changes in abundance in tissues and circulation during disease. This study aimed to explore differences in plasma miRNA abundance between patients with NSII and IP. Plasma samples were collected from 43 horses, consisting of 21 with NSII and 22 with IP. A subset (n = 12) was submitted for deep small RNA sequencing to identify miRNAs differing between the groups. Next, a panel of nine miRNAs (two were potential normalizers) were selected for evaluation and confirmation by reverse transcription quantitative real-time PCR (RT-qPCR). Small RNA sequencing detected 628 miRNAs in the blood samples, but no miRNAs were differentially abundant between the disease groups. This finding was confirmed by qPCR. In agreement with previous studies, the top abundant miRNAs in both groups included Eca-Mir-122–5p and Eca-Mir-486–5p, as well as Eca-Mir-223–3p, which has previously been associated with inflammation. Target prediction for the most abundant miRNAs additionally predicted targets in inflammatory pathways. Evaluation of clinicopathological parameters revealed differences between the groups in two measures (white blood cell count and blood neutrophil count), which aligns with findings from previous studies. The results demonstrate that NSII and IP elicit similar miRNA profiles in plasma and are characterized by systemic inflammation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106378"},"PeriodicalIF":2.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}