Evaluating the role of lyophilized hydatid cyst fluid in enhancing skin wound healing: In Vitro and In Vivo rat model studies.

Skin plays a vital role in maintaining various physiological functions, including barrier protection, temperature regulation, and sensory perception. Effective wound healing is crucial for restoring tissue integrity after injury, involving a complex interplay of cellular and molecular mechanisms. Hydatid cyst fluid (HCF), derived from Echinococcus granulosus, contains bioactive components that may enhance wound healing. This study evaluates the potential of lyophilized HCF to promote skin wound repair using an in vitro assay and an in vivo rat wound model. Lyophilized HCF was prepared from hydatid cyst fluid obtained from the lungs of infected camels and analyzed using gas chromatography-mass spectrometry (GC-MS). Cytotoxicity was assessed using the MTT assay (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) on endothelial and fibroblast cell lines. An in vivo full-thickness skin defect model was created in rats, and wounds were treated with either lyophilized HCF or saline (control). Wound closure rates were measured on days 7 and 21, and histological evaluations were conducted using standard techniques. GC-MS analysis revealed that lyophilized HCF contains bioactive compounds, including antimicrobial agents, fatty acids, and molecules that promote angiogenesis. In vitro MTT assay confirmed that lyophilized HCF exhibited no cytotoxicity and supported cell viability. In vivo results revealed significantly improved wound closure in the HCF-treated group compared to control groups. Specifically, in the control group, the wound closure was 23.52 % ± 4.23 on day 7 and 86.02 % ± 1.08 on day 21. In the lyophilized HCF group, the closure was significantly higher, with 48.99 % ± 6.12 on day 7 and 91.13 % ± 1.9 on day 21. Histological analysis revealed that the HCF-treated wounds exhibited significantly improved epithelialization (p = 0.0211 on day 7; p = 0.0003 on day 21), reduced inflammatory cell infiltration (p = 0.0277 on day 7; p = 0.0179 on day 21), enhanced collagen deposition (p = 0.0082 on day 7; p = 0.0127 on day 21), and increased angiogenesis (p = 0.0001 on day 7; p < 0.0001 on day 21), compared to the control group. In conclusion, lyophilized HCF promotes effective wound healing through its bioactive components, supporting cell proliferation, reducing inflammation, and enhancing collagen deposition and angiogenesis. These findings suggest that HCF could serve as a promising therapeutic agent for wound repair. Further studies are warranted to explore its clinical applications.