Veterinary journal最新文献

{"title":"Urinary lipid production in dogs with urothelial carcinoma","authors":"Akane Hayashi ,&nbsp;Shingo Maeda ,&nbsp;Arisa Yamazaki ,&nbsp;Tatsuro Nakamura ,&nbsp;Yuko Goto-Koshino ,&nbsp;Tomohiro Yonezawa ,&nbsp;Koji Kobayashi ,&nbsp;Takahisa Murata","doi":"10.1016/j.tvjl.2025.106373","DOIUrl":"10.1016/j.tvjl.2025.106373","url":null,"abstract":"<div><div>Canine urothelial carcinoma (cUC) is a prevalent and aggressive malignancy in dogs. Current treatments have limited effectiveness, and delays in diagnosis are often a concern. cUC is unique in that it shows high responsiveness to cyclooxygenase (COX) inhibitors and has a high frequency of a single nucleotide mutation in <em>BRAF</em>, known as <em>BRAF</em>^{<em>V595E</em>}. COX is one of the key enzymes involved in the production of lipid mediators, which regulate the progression of various diseases. Although studies have revealed the roles of COX in cUC, the production profiles of lipid mediators remain unknown. In this study, we comprehensively measured the urinary lipid mediator levels of cUC dogs using liquid chromatography-tandem mass spectrometry. We found that lipid production was altered in cUC, with increased amounts of several COX-catalyzed lipids, including prostaglandin E₂ and thromboxane B₂, and several lipoxygenase-catalyzed lipids, including leukotriene E₄. Additionally, we found that the <em>BRAF</em>^{<em>V595E</em>} mutation tended to increase the levels of most enzymatically produced lipids. Our results provide insights into novel therapeutic and diagnostic strategies for cUC and cancers with <em>BRAF</em> mutations.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106373"},"PeriodicalIF":2.3,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of prepartum clostridial vaccine administration with mitigation of respiratory and gastrointestinal disorders in calves","authors":"Shin Nakayama ,&nbsp;Daiki Aomori ,&nbsp;Fumika Mune ,&nbsp;Hiroaki Sato ,&nbsp;Osamu Nishino ,&nbsp;Shinichi Tose","doi":"10.1016/j.tvjl.2025.106368","DOIUrl":"10.1016/j.tvjl.2025.106368","url":null,"abstract":"<div><div>Pathogenic clostridial infections, particularly those involving <em>Clostridium perfringens</em> type A (CpA)-derived alpha-toxin (CpA-α), are a recognized cause of gastrointestinal disorders in young ruminants. However, the potential role of anti-clostridial antibodies in mitigating not only gastrointestinal but also respiratory disorders in calves remains poorly investigated under field conditions. This study evaluated the effects of anti-clostridial antibodies passively transferred from cows vaccinated with a clostridial vaccine (CV) during the prepartum period on gastrointestinal and respiratory disorders in nursing calves. Cows in the CV group were vaccinated at 8 and 4 weeks before parturition, whereas control cows were not. Colostrum intake was verified in calves from both groups. Serum antibody titers against clostridial and bovine respiratory disease (BRD) pathogens were measured. Treatment frequency and fecal consistency were also recorded. Anti-clostridial antibody titers were higher in CV cows and their calves compared to the control group. In the CV group, the number of respiratory treatments in calves showed a decreasing trend from 5 to 8 weeks of age and was significantly lower from 9 to 12 weeks of age. Fecal consistency at 1 week of age was also significantly improved in the CV group. The number of respiratory treatments was negatively correlated with anti-CpA-α antibody titers in calves, but not with anti-BRD titers. These findings suggest that prepartum CV vaccination may help mitigate both respiratory and gastrointestinal disorders in nursing calves, potentially reducing antibiotic use and lowering the risk of antimicrobial resistance in livestock industry.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106368"},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inducing and measuring positive affective state in domesticated equines: A Delphi consultation","authors":"Loni Loftus ,&nbsp;Lucy Asher ,&nbsp;Matthew Leach","doi":"10.1016/j.tvjl.2025.106370","DOIUrl":"10.1016/j.tvjl.2025.106370","url":null,"abstract":"<div><div>Over the last twenty years the definition of good animal welfare has advanced from the ‘absence of negative welfare states’ to the aim of identifying the presence of positive welfare states; however, research on positive animal welfare is relatively new. Consequently, through expert consultation, this study aimed to synthesise knowledge regarding domesticated equine emotional state, specifically methods to induce and measure positive affective states, which could be of significant benefit to equine welfare across sectors. A Delphi consultation of experts in the field of equine behaviour, welfare and affective state was undertaken to derive consensus agreement on definitions used when considering positive affective state in equines and methods suitable for inducing and measuring these states within experimental investigations. Ninety-three international experts participated in a robust three-round Delphi consultation with data gathered analysed qualitatively (thematic analysis) and quantitatively (ranking data, consensus benchmarks and Content Validity Index (CVI) / Content Validity Ratio (CVR) analyses). Retention rates were high (78 %), and consensus (minimum 70 % agreement) was reached within Round three of the consultation. Nineteen methods for inducing positive affect reached consensus (provision of high value food, an affiliative companion and a substrate to roll in where the highest ranked), and twelve behavioural (assessment of body language, facial actions and horse-horse interactions were ranked highest) and three physiological (evaluation of heart rate, heart rate variability and respiratory rate) variables for measuring positive affect also reached consensus. This consultation highlights several important considerations surrounding the induction and measurement of positive affective state in equines, including consideration of the individual’s personality and individual preference within any measures of affective state. We therefore recommend that establishing individual preference should be a prerequisite of research into positive affective states. It is anticipated that the results of this study can be used to provide new direction for research on positive affective states by providing expert agreed methods and measures for policy and practice through expert agreed approaches to induce positive affect in horses.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106370"},"PeriodicalIF":2.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra and postoperative effects of adding detomidine to a lidocaine paravertebral anaesthesia in cows undergoing standing abomasum displacement surgery","authors":"O. d’Anselme ,&nbsp;A. Hartnack ,&nbsp;O. Bühlmann ,&nbsp;P.R. Torgerson ,&nbsp;E. Kolp ,&nbsp;S.K. Ringer","doi":"10.1016/j.tvjl.2025.106364","DOIUrl":"10.1016/j.tvjl.2025.106364","url":null,"abstract":"<div><div>This study investigated the benefits of adding detomidine to a lidocaine proximal paravertebral nerve block (PPNB) for standing laparotomy in cattle. Forty adult cows undergoing surgery for abomasal displacement were randomly assigned to receive a PPNB with lidocaine (LIDO: 40 ml 2 % lidocaine per injection site) or lidocaine-detomidine (LIDO-DETO: lidocaine + 5 μg/kg detomidine equally distributed to the lidocaine syringes). The PPNB was done at T13, L1 and L2 vertebra, always by the same investigator using a blind technique. Intraoperative sedation score, pain score, heart (HR) and respiratory rate (RR) were recorded before PPNB and repeatedly after (5, 10, 15 min, then every 15 min) by a blinded observer (ODA). Rescue analgesia (local lidocaine infiltration) was applied based on the blinded surgeon’s decision. Quality of anaesthesia was also assessed by the surgeons using a visual analogue scale (VAS) at the end of the intervention. Postoperative pain was evaluated (ODA) using the Zürich Composite Measure Pain Scale (ZCMPS) and mechanical nociceptive threshold (MNT) testing at 0, 2, 6, 12, and 24 hours. Surgery was completed in all cows, though two (LIDO-DETO) went into sternal recumbency. LIDO-DETO resulted in superior VAS (P &lt; 0.001), higher sedation (P &lt; 0.001), lower pain scores (P = 0.001), and reduced RR (P = 0.015). Postoperatively, LIDO-DETO cows showed significantly lower ZCMPS (P = 0.048) and higher MNT on the wound's left side (P = 0.026). Adding detomidine to lidocaine PPNB improved intraoperative anaesthesia quality with potential postoperative analgesic benefits.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106364"},"PeriodicalIF":2.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143900329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, diagnosis, treatment outcomes and immunohistochemical characterization of neoplastic disorders in reptiles presented at a veterinary teaching hospital: A cross-sectional study (2010–2023)","authors":"F. Solanes-Vilanova,&nbsp;T. Hellebuyck,&nbsp;K. Chiers","doi":"10.1016/j.tvjl.2025.106366","DOIUrl":"10.1016/j.tvjl.2025.106366","url":null,"abstract":"<div><div>Neoplasms seem to be increasingly encountered in reptile medicine. The present study investigated the prevalence, diagnosis, treatment outcome and immunohistochemical characterization of neoplasms diagnosed in reptile patients presented at a veterinary teaching hospital between 2010 and 2023. In this cross-sectional study, we analyzed the clinical records of 12383 reptiles (2549 snakes, 3386 lizards, and 6448 chelonians). Within the study population, 7261 reptiles showed clinical signs and in 130 of these cases, a histological diagnosis of neoplasm was made. A total of 134 neoplasms were identified in 27 out of 3270 chelonians (0.83 %), 63 out of 2395 lizards (2.63 %), and 40 out of 1596 snakes (2.51 %). While dermal neoplasms (n = 59; 44 %) such as squamous and basal cell carcinoma, were the most common neoplasms in lizards and chelonians, snakes were most frequently diagnosed with alimentary tract neoplasms (n = 35; 26.1 %), particularly tubular adenocarcinomas. Integumentary, reproductive and oral neoplasms carried a good prognosis following complete surgical removal, in contrast to most other neoplasms, particularly those with malignant, multicentric, or metastatic features. The results of this study elucidate the diversity of neoplastic disorders that can be encountered in reptiles within a clinical setting. In addition, similarities and differences with previously published retrospective studies originating from specialty diagnostic services and species-specific predispositions were investigated. Although histological evaluation allowed the characterization and classification of a large number of the observed neoplasms, the application of appropriate immunohistochemical protocols was indispensable towards accurate identification of several neoplasms, particularly neoplasms originating from the hematopoietic system and soft tissue sarcomas.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106366"},"PeriodicalIF":2.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Racing performance in 75 Thoroughbreds after arthroscopic removal of Osteochondritis dissecans from the lateral femoral trochlear ridge before first race start in Korea (2015–2017)","authors":"Y. Sohn ,&nbsp;S.J. An ,&nbsp;E. Forbes ,&nbsp;B.S. Kim ,&nbsp;H.S. Jeon ,&nbsp;S.-H. Ryu ,&nbsp;I. Lee","doi":"10.1016/j.tvjl.2025.106367","DOIUrl":"10.1016/j.tvjl.2025.106367","url":null,"abstract":"<div><div>The lateral trochlear ridge of the stifle joint is highly susceptible to developing Osteochondrosis (OC)/Osteochondritis dissecans (OCD) lesions. This retrospective study evaluated the medical and racing outcomes of 75 Thoroughbreds who underwent arthroscopic OCD removal at the Jeju Stud Farm Equine Hospital from 2015 to 2017. The analysis included medical records, radiographic assessments, and race performance data, comparing 75 horses that underwent surgery with 257 control horses selected as siblings from the same dam. The surgical group had comparable racehorse registration rates (89.3 %) and first race start rates (72.0 %) compared to those of the control group (84.8 % and 74.7 %, respectively). However, horses that underwent surgery had their first race start later (<em>p</em> = 0.000864, <em>p</em> &lt; 0.0001) and participated in fewer total races (<em>p</em> = 0.01708, <em>p</em> &lt; 0.05) than the control group. Despite these differences, there were no significant variations in sales prices, career earnings, race points, or retirement age. Furthermore, neither the size of the OCD lesion nor the timing of the surgery significantly influenced overall racing performance metrics, except for a reduction in career duration for horses operated on at an older age. Sex-based analyses revealed that male horses consistently outperformed females in career duration and earnings. Subgroup analyses revealed significantly poorer outcomes in females treated before 12 months of age and those with large lesions (≥ 40 mm), including fewer starts and earlier retirement. In summary, arthroscopic surgery for stifle OCD in Thoroughbreds before their first race start did not negatively affect overall performance. While lesion size and surgery timing had minimal impact, this study suggests that sex-related factors may more strongly influence post-surgical outcomes.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106367"},"PeriodicalIF":2.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of altered distances between A-frame and the preceding jump on front limb dynamics in agility dogs","authors":"Claire Bidwell ,&nbsp;Scott Blake ,&nbsp;David Marlin ,&nbsp;Roberta Blake","doi":"10.1016/j.tvjl.2025.106363","DOIUrl":"10.1016/j.tvjl.2025.106363","url":null,"abstract":"<div><div>A high proportion of agility injuries associated with an obstacle are due to the A-frame, however, there is limited research into the kinetics and kinematics of dogs traversing this type of equipment. The aim of this research was to study the kinematics and kinetics of agility dogs negotiating an A-frame when the preceding obstacle (in this case a jump) was placed at 10 m, 7.5 m and 5 m ahead of the A-frame. Six competition standard agility dogs were recorded negotiating an A-frame after completing a jump with each dog attempting each distance three times. Inertial measuring units attached to each dog gathered maximum velocity, acceleration and deceleration between jump landing and the A-frame. Video analysis and pressure sensors gathered carpal hyperextension and peak vertical forces for both forelimbs at the dogs’ contact with the A-frame. The study found no difference in either carpal extension or PVF data between the different distances. However, maximum approach velocity decreased (p &lt; 0.05) with decreasing distance: 10 m (7.30 ± 0.40 m/s), 7 m (6.61 ± 0.34 m/s), and 5 m (5.74 ± 0.62 m/s). Acceleration was also decreased at the 5 m distance compared with 10 m distance (p &lt; 0.05). A notable finding was the −1.57 m/s² decrease in deceleration found between the 10 m (-5.92 m/s²) and 5 m (-4.35 m/s²) distances (p &lt; 0.05), with the 10 m distance having 36 % more deceleration than 5 m. As forelimbs have a role in deceleration, an increased distance between obstacles could be one of the factors involved in forelimbs injuries in agility dogs. In our study, positioning the preceding obstacle 5 m from the A-frame moderated speed, acceleration, and deceleration, and could potentially help to reduce reported injury rates, but additional studies are recommended to allow evidence-based guidelines.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106363"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population pharmacokinetics and clinical evaluation of intravenous acetaminophen and its metabolites in Andalusian horses","authors":"M.M. Granados ,&nbsp;F. Medina-Bautista ,&nbsp;R. Navarrete-Calvo ,&nbsp;D. Argüelles ,&nbsp;J.M. Domínguez-Pérez ,&nbsp;F. Priego-Capote ,&nbsp;P. Muñoz-Rascón ,&nbsp;J.M. Serrano-Rodríguez","doi":"10.1016/j.tvjl.2025.106357","DOIUrl":"10.1016/j.tvjl.2025.106357","url":null,"abstract":"<div><div>To date, no intravenous pharmacokinetics (PK) studies have assessed acetaminophen or its major metabolites (acetaminophen-glucuronide and acetaminophen-sulphate) in horses. The influence of sex on acetaminophen metabolism in horses is unclear, and Monte Carlo simulations have not been applied to explore potential clinical applications. This study aimed to determine the intravenous PK and safety of acetaminophen in Andalusian horses at 10 and 20 mg/kg, evaluate sex as a covariate, and simulate clinical regimens. Twenty mares and twenty stallions received intravenous acetaminophen, and plasma concentrations were analysed by LC-MS/MS and modelled using non-linear mixed-effects models (MonolixSuite®). Safety was evaluated via clinical examinations and by haematological and biochemical profiles 24 h after administration. Two constant-rate infusion (CRI) regimens were examined in PK simulations that did not involve surgery: a short-duration, high-dose infusion (3 h) for peri-operative scenarios (short-infusion group at 3.33, 6.66, and 10.0 mg/kg/h) and a longer-duration, lower-dose infusion (8 h) for post-operative analgesia scenarios (long-infusion group at 1.25, 2.50, and 3.75 mg/kg/h). After modelling, glucuronide and sulphate levels were 2.1 and 4.53 times higher, respectively, than acetaminophen levels. Half-lives were 3.5, 5.6, and 3.2 h for acetaminophen, glucuronide, and sulphate, respectively. Clearance was influenced by sex (mares: 0.47 L/h/kg; stallions: 0.35 L/h/kg). No adverse effects were observed. Steady-state concentrations observed in the CRI simulations ranged from 1.75–5.21 µg/mL (short-infusion group) and 0.62–2.02 µg/mL (long-infusion group). Acetaminophen exhibited sex-dependent PK variability and high-level formation of metabolites. These findings support further clinical evaluation of acetaminophen CRIs in equine analgesia.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106357"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis and spatiotemporal distribution of newly emerging lumpy skin disease virus in malaysia identify recombinant strain from transboundary spread","authors":"Nurain Izzati Saidi,&nbsp;Nur Athirah Azhar,&nbsp;Mohamad Azlan Jahaya,&nbsp;Mohd. Shafarin Shamsuddin,&nbsp;Syahrol Zamzuri","doi":"10.1016/j.tvjl.2025.106365","DOIUrl":"10.1016/j.tvjl.2025.106365","url":null,"abstract":"<div><div>Lumpy skin disease is a transboundary viral disease affecting bovine species. This disease had never been recorded in Malaysia prior to May of 2021, and had spread to 65 of 92 districts in Peninsular Malaysia by December of the same year. In this study, we conducted a whole-genome sequencing, phylogenetic analysis and geographical mapping of the outbreak from lumpy skin disease virus isolated from the first wave of outbreaks to better understand the origin, transmission, and genetic diversity of the virus. The whole genome sequences of these isolates were deposited in GenBank with accession number PP145891 and PP145892. Malaysia LSDV isolates were found clustered with recombinant strains in clade 2.5, particularly subclade 2.5.1, which included strains from China, Vietnam, Thailand, and Indonesia. The spread of the disease in Malaysia was closely linked to cross-border cattle movements from Thailand, as evidenced by the spatiotemporal distribution of the outbreaks. This study emphasizes the need for ongoing genomic surveillance to detect emerging variants and inform targeted control strategies, including vaccination and cross-border cooperation to curb future outbreaks.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106365"},"PeriodicalIF":2.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral fluid collection in wild boar: A field protocol","authors":"David Relimpio ,&nbsp;Aleksandra Kosowska ,&nbsp;Sandra Barroso-Arévalo ,&nbsp;Daniel De Antonio-Gómez ,&nbsp;Christian Gortázar ,&nbsp;Jose A. Barasona","doi":"10.1016/j.tvjl.2025.106362","DOIUrl":"10.1016/j.tvjl.2025.106362","url":null,"abstract":"<div><div>Traditional wildlife sampling methods have limitations in providing a well-distributed, timely and accurate disease surveillance in wildlife. We developed a field protocol for collecting wild boar (<em>Sus scrofa</em>) oral fluid samples for non-invasive active disease surveillance using environmental ropes. We deployed ropes in field settings and tested the rope-sampled oral fluids for antibodies against <em>Mycobacterium bovis</em> and closely related members of the <em>Mycobacterium tuberculosis</em> complex (MTC). Oral fluid samples were collected from three wild boars populations in Spain with endemic tuberculosis across two seasons with contrasting natural food availability. Additionally, we studied the effect of placing the ropes close to aggregation points and of impregnating the ropes with corn powder. The results suggested that installing environmental ropes in summer, close to feeders or aggregation points, and baited with corn powder significantly increases the proportion of bitten ropes. No significant differences were observed in the effectiveness of ropes for oral fluid sampling across the three study sites. The average rate of antibody detection by ELISA was 38 % (range 34–45 %) of the bitten ropes. This method appears to be both precise and sensitive in detecting antibodies against MTC in oral fluid samples collected from environmental ropes, making it a potentially valuable tool for early detection and monitoring of MTC circulation. This noninvasive sampling method can easily be adapted for other relevant diseases of wild suids including African swine fever. Therefore, environmental ropes represent an effective, inexpensive, flexible, environmentally friendly, and noninvasive alternative for active wildlife disease surveillance.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106362"},"PeriodicalIF":2.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}