Veterinary journal最新文献

{"title":"Field-deployable molecular workflow for detection, resistance screening, and genotyping of Staphylococcus aureus in bovine mastitis","authors":"Tewodros Fentahun Jember ,&nbsp;Mark E. Westman ,&nbsp;Sameer Dinkar Pant ,&nbsp;Seyed Ali Ghorashi","doi":"10.1016/j.tvjl.2026.106583","DOIUrl":"10.1016/j.tvjl.2026.106583","url":null,"abstract":"<div><div><em>Staphylococcus aureus</em> (<em>S. aureus</em>) is a common cause of cow-associated bovine mastitis and continues to pose a challenge in dairy herds. Early and accurate diagnosis is essential for effective control and treatment of the infection. This study developed a field-adapted molecular workflow combining a simple DNA extraction method with a colourimetric loop-mediated isothermal amplification (LAMP) assay targeting the <em>nuc</em> gene for <em>S. aureus</em> detection, and a second LAMP assay targeting the <em>mecA</em> gene to identify methicillin resistance. LAMP results were benchmarked against PCR using 14 milk samples confirmed as <em>S. aureus</em>-positive by culture and MALDI-TOF MS. A secondary aim of the study was to investigate the genetic diversity of <em>S. aureus</em> isolates from cases of bovine mastitis in Australia by analysis of the spa gene. The spa genotyping was carried out using PCR followed by high-resolution melt (HRM) curve analysis, supported by a mathematical model based on genotype confidence percentage (GCP) values and validated by DNA sequencing. The <em>nuc</em>-LAMP assay demonstrated 89.5 % sensitivity and 100 % specificity relative to PCR, while the <em>mecA</em>-LAMP assay showed complete agreement with PCR in identifying methicillin-susceptible isolates. Among 14 positive milk samples, nine distinct <em>spa</em> types were identified by sequencing, reflecting high genetic diversity. The GCP-based model provided a reproducible and objective method for assigning <em>spa</em> types, even when melt curve differences were subtle. This integrated, low-cost approach offers a practical solution for point-of-care mastitis diagnostics and antimicrobial resistance monitoring using LAMP, particularly suited to resource-limited and remote farm settings, followed by laboratory-based spa genotyping through HRM for strain characterisation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106583"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of the prevalence and production impact of Mycobacterium avium subsp. paratuberculosis (MAP) infection in the sheep populations of Great Britain and New Zealand","authors":"Laura Worsley ,&nbsp;Anne Ridler ,&nbsp;Amy Wedley ,&nbsp;Joanna Urbaniec ,&nbsp;Peers Davies","doi":"10.1016/j.tvjl.2026.106600","DOIUrl":"10.1016/j.tvjl.2026.106600","url":null,"abstract":"<div><div><em>Mycobacterium avium subspecies paratuberculosis</em> (MAP) is a prevalent, obligate pathogen of many wild and domestic species with a global distribution. MAP prevalence, epidemiology and production impact has been extensively studied in cattle whilst comparatively little research has been conducted on sheep. The aim of this project was to assess the prevalence of MAP in Great Britain (GB) and New Zealand (NZ) using faecal PCR and describe the relationship between MAP faecal shedding, reproductive failure (as determined by ultrasound pregnancy diagnosis) and longevity. A total of 162 farms in GB and 76 in NZ were sampled using a combination of individual and pooled faecal sampling frames of which 70 % of GB flocks and 88 % of NZ flocks tested positive for MAP. MAP shedding was associated with a 3 times increased risk of reproductive failure and significantly higher flock replacement rates in infected flocks. Reproductive failure is an important cause of forced culling in commercial flocks, leading to higher replacement rates and reduced lifetime output per breeding ewe. Excess culling and replacement costs due to preventable infectious disease reduce flock productivity, profitability and negatively impact sustainable lamb production. Effective control measures are available for MAP infected flocks but adoption is hampered primarily, but not exclusively, by poor recognition and underdiagnosis of the disease coupled with a poor awareness of the production limiting nature of the pathogen at the flock level.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106600"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146221043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EEG-based quantification of chronic pain in cats: A proof-of-concept study using the Piq algorithm","authors":"Aliénor Delsart ,&nbsp;Colince Segning ,&nbsp;Aude Castel ,&nbsp;Colombe Otis ,&nbsp;Guillaume Dumas ,&nbsp;Maxim Moreau ,&nbsp;Bertrand Lussier ,&nbsp;Rubens Da Silva ,&nbsp;Karen Barros Parron Fernandes ,&nbsp;Johanne Martel-Pelletier ,&nbsp;Jean-Pierre Pelletier ,&nbsp;Eric Troncy ,&nbsp;Suzy Ngomo","doi":"10.1016/j.tvjl.2026.106608","DOIUrl":"10.1016/j.tvjl.2026.106608","url":null,"abstract":"<div><div>While chronic pain assessment in household pets remains challenging, the use of non-invasive electroencephalography (EEG) in cats has shown promise to identify pain more objectively in this species. A novel EEG-based algorithm – Pain identification and quantification (Piq) – was originally developed in humans to quantify pain intensity. In this proof-of-concept study, the objective was to evaluate whether the Piq algorithm could be explored for feasibility to identify and quantify chronic osteoarthritic (OA) pain in cats. Adult neutered cats (<em>n</em> = 5 including <em>n</em> = 2 with osteoarthritis, OA) were assessed for their functional impairment (Montreal instrument for cat arthritis testing for use by veterinarians, MI-CAT(V)) and neuro-sensitization at both peripheral (Paw Withdrawal Threshold, PWT) and spinal (response to mechanical temporal summation, RMTS) levels. Resting-state EEG recordings were acquired from Cz, C3/C4 under conscious and sedated conditions. The first five minutes of EEG data were analyzed using the Piq algorithm, with Piq scores ≥ 10 % used as an exploratory threshold transferred from human studies. Pain-free cats showed gamma frequency band Piq scores &lt; 10 % while OA cats exceeded 10 % in both conscious and sedated conditions at Cz. Piq scores were negatively correlated with PWT, suggesting an increased neuro-sensitization with higher Piq scores. These preliminary findings suggest that the algorithm may capture gamma-band EEG patterns potentially associated with chronic OA pain in cats, consistent with prior evidence in humans. Despite the small sample size, this study demonstrates the feasibility of applying a human EEG-based pain quantification algorithm to OA cats, supporting its potential for future cross-species translation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106608"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147272042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical comparison of three locking plate constructs for stabilization of a femoral segmental defect model in cats","authors":"Jeniffer Gabriela Figueroa Coris ,&nbsp;Sheila Canevese Rahal ,&nbsp;César Augusto M. Pereira ,&nbsp;Guilherme Rech Cassanego ,&nbsp;Teng Hsiang Wei ,&nbsp;Fátima Maria C. Caldeira ,&nbsp;Marcelo José Carbonari","doi":"10.1016/j.tvjl.2026.106589","DOIUrl":"10.1016/j.tvjl.2026.106589","url":null,"abstract":"<div><div>This study biomechanically evaluated three titanium locking plate constructs for stabilizing a segmental defect model that simulated a comminuted femoral shaft fracture in cats. Four groups were formed: G1 – single locking plate; G2 – plate-rod construct; G3 – dual plate in an orthogonal configuration; and G4 – intact bone. G1 used 16-hole locking plate with bicortical screws placed in holes 1, 3, 7, 10, 14, and 16. In G2, an intramedullary pin was included, with monocortical screws in the same positions. In G3, screws were placed as in G1, except that monocortical screws were used in holes 7 and 10. Additionally, an 8-hole plate was applied in orthogonal configuration, with monocortical screws placed in holes 1, 3, 6, and 8. All constructs underwent cyclic eccentric axial compression, followed by destructive testing, except G4, which was tested only destructively. During cyclic testing, stiffness did not differ between G2 and G3, while G1 exhibited lower stiffness. Deformation measured with Gauge 1 (16-hole plate) was greatest in G1, with no significant difference between G2 and G3. Deformation measured with Gauge 2 (intramedullary pin and orthogonal plate) was greater in G2 than in G3. In destructive testing, stiffness did not differ between G1 and G2, or between G2 and G3. Maximum force and displacement did not differ among Groups 1, 2 and 3. Group 4 differ from other groups for these variables. In conclusion, both the locking plate–rod construct and the dual titanium locking plate demonstrated similar stiffness, but locking plate–rod configuration exhibited greater deformation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106589"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TGF-β1 and estradiol modulate prostaglandin concentrations and related gene expression in equine endometrium during the follicular phase","authors":"Ana Amaral ,&nbsp;Marta Cerveira-Pinto ,&nbsp;Carina Santos ,&nbsp;Pawel Kordowitzki ,&nbsp;Dariusz Skarzynski ,&nbsp;Graça Ferreira-Dias ,&nbsp;Anna Szóstek-Mioduchowska","doi":"10.1016/j.tvjl.2026.106594","DOIUrl":"10.1016/j.tvjl.2026.106594","url":null,"abstract":"<div><div>Transforming growth factor (TGF)-β1 is a pro-fibrotic cytokine that affects extracellular matrix (ECM) deposition and fibroblast activity. 17β-Estradiol (E₂), the dominant ovarian steroid during the follicular phase (FLP) of the estrous cycle, can also influence ECM remodeling and fibrosis, through prostaglandin (PG) synthesis. PGs have opposing roles in fibrosis, with PGE₂ showing anti-fibrotic effects and PGF₂α promoting fibrosis. Equine endometrosis, whose main pathological feature is fibrosis, is marked by chronic inflammation and ECM accumulation, and may involve mediators like TGF-β1, PGs, and E₂. This study aimed to assess how TGF-β1, E₂, and their combination affect PG synthase and receptors transcription (qPCR) and PG concentrations (ELISA) in equine endometrial explants during the FLP, after 24 and 48 h. Prostaglandin-endoperoxide synthase 2 (<em>PTGS</em>-2) mRNA was reduced with TGF-β1 and combination treatments at 24 h. Estradiol and combined treatments downregulated microsomal prostaglandin E synthase1 (<em>PGES</em>) mRNA at 24 h, while prostaglandin F synthase (<em>PGFS</em>) mRNA reduced with TGF-β1 at 24 h and with E₂ at 48 h. The PGE₂ concentration was lower in TGF-β1 +E₂ group than in controls and TGF-β1 alone at 48 h. In contrast, PGF₂α concentration increased with E₂ at 24 h and TGF-β1 and TGF-β1 +E₂ treatments at 48 h. Prostaglandin E receptor (<em>EP</em>)<em>2</em> and <em>4</em> mRNA upregulated with the combination treatment, while prostaglandin F receptor (<em>FP</em>) mRNA decreased in all treated groups. These findings suggest that TGF-β1 and E₂ interact to regulate PG pathways, with potential to drive fibrotic changes in the equine endometrium, by shifting the balance between anti- and pro-fibrotic mediators like PGE₂ and PGF₂α.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106594"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146221177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of intravenous rehydration protocols based on an acetate-containing electrolyte solution, isotonic saline, and an isotonic mixture of saline/sodium bicarbonate on acid-base status and plasma electrolyte concentrations in calves with diarrhoea","authors":"Dana Badura ,&nbsp;Annette Lorch ,&nbsp;Andrea Urgibl-Bauer ,&nbsp;Antonia Kreuzer ,&nbsp;Ingrid Lorenz ,&nbsp;Yury Zablotski ,&nbsp;Florian M. Trefz","doi":"10.1016/j.tvjl.2026.106571","DOIUrl":"10.1016/j.tvjl.2026.106571","url":null,"abstract":"<div><div>Strong ion (metabolic) acidosis, dehydration, and electrolyte imbalances are common in neonatal calves with diarrhoea. Balanced crystalloids may provide advantages for rehydration purposes in those animals due to their polyionic composition and higher buffer capacity compared to conventional buffer-free fluids such as 0.9 % NaCl. This randomized clinical trial aimed to compare the effects of three infusion solutions - an acetate-containing balanced electrolyte solution (Sterofundin® ISO; Stero), 0.9 % saline (NaCl), and an isotonic saline-sodium bicarbonate mixture (NaClBic) - on acid-base status and plasma electrolyte concentrations in neonatal calves with diarrhoea. Thirty-three calves with diarrhoea, metabolic acidosis (plasma HCO₃^– &lt; 25 mmol/L), and signs of clinical dehydration were enrolled. After initial correction of metabolic acidosis using 5.6 % sodium bicarbonate over 55 min, each of 11 calves randomly received one of the three infusion solutions at 8 mL/kg/h for 22 h. All three crystalloids had an acidifying effect, which was most pronounced in the NaCl group and least in the NaClBic group. At 18 and 24 h after initiation of treatment, plasma bicarbonate concentrations were significantly lower (<em>P</em> &lt; 0.05) in the Stero and NaCl groups compared to the NaClBic group. Hyperchloraemia was observed in calves treated with 0.9 % NaCl. No statistically significant differences in clinical variables or plasma potassium, magnesium, and calcium concentrations were observed between groups after 24 h. In conclusion, the acetate-containing balanced solution did not provide a clinically relevant advantage over NaCl and NaClBic. However, findings support the use of chloride-restricted solutions for rehydration purposes in dehydrated neonatal calves with diarrhoea.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106571"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of paraoxonase-1 activity and cupric reducing antioxidant capacity in cerebrospinal fluid of dogs with neurological disorders","authors":"María Gemma Velasco-Martínez ,&nbsp;Ángel María Hernández-Guerra ,&nbsp;Antonio Campos Medina ,&nbsp;Deborah Chicharro ,&nbsp;Belén Cuervo ,&nbsp;Joaquín Jesús Sopena ,&nbsp;Mónica Rubio ,&nbsp;Luis Pardo-Marín ,&nbsp;José Joaquín Cerón ,&nbsp;Katiuska Satué","doi":"10.1016/j.tvjl.2026.106591","DOIUrl":"10.1016/j.tvjl.2026.106591","url":null,"abstract":"<div><div>Cerebrospinal fluid (CSF) is a liquid that remains in close contact with the central nervous system (CNS) and is therefore commonly used to identify pathological alterations related to CNS diseases. However, sometimes techniques usually employed in clinical practice do not reveal alterations and other parameters, such as paraoxonase-1 (PON1) activity and total antioxidant capacity (TAC), are needed in this field. In this paper PON1 activity and TAC estimated by the cupric reducing antioxidant capacity (CUPRAC) method are analyzed in CSF of dogs with idiopathic epilepsy (IE), intracranial tumors (IT), inflammatory-based pathologies of the CNS (INF) as well as acute (AH) and chronic intervertebral disc herniations (CH). Analyses were performed within a maximum of three months after sample collection. No differences in CUPRAC values were found between the groups; however, the results revealed significantly higher values of PON1 activity in INF group compared to IE (P = 0.019), IT (P = 0.004) and AH (P = 0.038). Since the estimation of PON1 activity in CSF appears to be a potentially useful tool in canine neurology, future studies are needed to elucidate its role in clinical practice.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106591"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146175107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble and insoluble fiber blend improves farrowing performance and colostral immunoglobulin concentration in sows without altering fecal microbiota","authors":"Bruno Bracco Donatelli Muro ,&nbsp;Rafaella Fernandes Carnevale ,&nbsp;Matheus Saliba Monteiro ,&nbsp;Diego Feitosa Leal ,&nbsp;André Pegoraro Poor ,&nbsp;Francisco Alves Pereira ,&nbsp;Vera Letticie de Azevedo Ruiz ,&nbsp;Cesar Augusto Pospissil Garbossa","doi":"10.1016/j.tvjl.2026.106574","DOIUrl":"10.1016/j.tvjl.2026.106574","url":null,"abstract":"<div><div>The aim of this trial was to provide a fibrous supplement in the transition diet of sows to decrease sows’ constipation, improve farrowing kinetics, increase colostrum antibodies, and modulate sows' and piglets' microbiota. At day 90 of pregnancy sows were fed a standard gestation diet until farrowing (CON, n = 101) or a standard gestation diet added of 400 g of a fibrous supplement (40 % wheat bran, 25 % lignocellulose, 25 % citrus pulp, and 10 % guar gum) (FIB, n = 101). After farrowing, sows were fed a standard lactation diet, and FIB sows were fed 400 g of the fibrous supplement on top of the feed for five days. Sows from FIB had a lower (p &lt; 0.01) incidence of constipation pre-farrowing and on the day of farrowing. Sows from FIB had a 23 min shorter (p = 0.01) farrowing duration compared to CON-sows. Sows from CON had a higher (p &lt; 0.01) feed intake on days two and three post-farrowing. Both IgG and IgA concentrations were higher in the FIB-supplemented sows (p = 0.01). The fecal microbiome of sows and piglets was similar (p &gt; 0.43) between groups. During the first week of life, piglets born from FIB-sows had decreased (p = 0.04) diarrhea incidence. In conclusion, the benefits of the fibrous supplement were evident in peripartal sows by decreasing farrowing duration and constipation and by increasing the concentration of immunoglobulins in the colostrum. For newborn piglets, the fibrous supplement fed to the sows did not significantly alter the fecal microbiome of sows and their litters. Nevertheless, benefits were observed in their resistance to neonatal diarrhea. Thus, using a fiber supplement in transition diets in farms may ease the management in the farrowing room and improve early-life robustness in piglets, which may decrease medication demands during the first week, contributing to more efficient neonatal care and better overall litter performance.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106574"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146133157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pinobanksin alleviates 4℃ semen preservation-induced oxidative damage and apoptosis in Wenchang Pig spermatozoa via the CaMKKβ/AMPK/Nrf2 pathway","authors":"Diqi Yang ,&nbsp;Shiwen He ,&nbsp;Xueqi Tian,&nbsp;Chang Cheng,&nbsp;Weiqin Fan,&nbsp;Hui Peng","doi":"10.1016/j.tvjl.2026.106599","DOIUrl":"10.1016/j.tvjl.2026.106599","url":null,"abstract":"<div><div>Artificial insemination underpins both commercial swine production and the conservation of critically endangered breeds. The Wenchang pig, which is classified as a critically endangered breed, faces a genetic bottleneck due to its reduced breeding population. Current estimates suggest that the breeding population has become quite limited, highlighting the need for effective low-temperature semen preservation strategies. Here, we report that inclusion of 1 μM pinobanksin—a propolis-derived dihydroflavonol—in standard extender sustains the functionality and viability of Wenchang pig spermatozoa during 15 days of storage at 4 °C. Compared to control, pinobanksin maintained total and progressive motility and path velocities (VAP, VCL, VSL), preserved membrane and acrosomal integrity, stabilized mitochondrial membrane potential, and prevented ATP and Ca²⁺ depletion. Biochemically, treated sperm exhibited reduced reactive oxygen species and malondialdehyde levels, restored glutathione homeostasis, elevated activities of SOD and GSH-PX, and upregulated expression of NQO1, HO-1 and GPX4. Mechanistic interrogation revealed that pinobanksin elevates intracellular Ca²⁺, promotes phosphorylation of CaMKKβ and AMPK, and increases cellular Nrf2 abundance. Pharmacological blockade of CaMKKβ (STO-609) or AMPK (Compound C) ablated pinobanksin’s antioxidant, anti-apoptotic and motility-preserving effects, whereas direct activation of Nrf2 (via TBHQ) reinstated sperm function under AMPK inhibition. These data delineate a coordinated signaling module involving CaMKKβ, AMPK and Nrf2 through which pinobanksin synchronizes redox and energy-metabolic defenses, establishing it as a dual-action cryoprotectant additive with significant promise for AI-based conservation of endangered porcine germplasm.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106599"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146207797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple preoperative biopsies may increase histologic grade accuracy in canine soft tissue sarcoma: a prospective study","authors":"Erica Ilaria Ferraris ,&nbsp;Lorella Maniscalco ,&nbsp;Eugenio Mazzone ,&nbsp;Matteo Olimpo ,&nbsp;Davide Giacobino ,&nbsp;Marina Martano ,&nbsp;Greta Martinelli ,&nbsp;Selina Iussich ,&nbsp;Emanuela Maria Morello","doi":"10.1016/j.tvjl.2026.106596","DOIUrl":"10.1016/j.tvjl.2026.106596","url":null,"abstract":"<div><div>Canine soft tissue sarcomas are mesenchymal tumors whose histologic grade predicts behavior and guides treatment. Pre-surgical biopsies may help in planning the surgical treatment and in improving prognostication. The concordance between pre-operative biopsy and final histology is reported to be about 59 %, which makes the value of the former questionable. The aim of this study was to evaluate if increasing the number of tumor biopsies may influence the concordance with the definitive histology. Thirty-two client-owned dogs with a cutaneous or subcutaneous soft tissue sarcoma were prospectively enrolled. After surgical removal, three punch biopsies were obtained from a central and two peripheral superficial areas. The incisional biopsies and the whole mass were then histologically evaluated, and a grade was assigned. The agreement between the single biopsy and the definitive histology varied depending on the location of the biopsy (peripheral vs central). It was 71 % for the central (K=0.47) and 59 % for the peripheral (K=0.33). Peripheral and central biopsies underestimated the definitive histology in 40.5 % and 29 % of cases, respectively. This study demonstrated that taking at least two biopsies from a peripheral and a central area of the tumor gives a higher chance of correctly predicting the definitive grade.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"316 ","pages":"Article 106596"},"PeriodicalIF":3.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}