Veterinary journal最新文献

{"title":"A novel lytic Seuratvirus phage CABI-SEA 2301 with broad host range against multidrug-resistant avian pathogenic Escherichia coli","authors":"Siwaporn Bilhman ,&nbsp;Sakkarin Lethongkam ,&nbsp;Anis Waeoh ,&nbsp;Arithat Limsatanun ,&nbsp;Jiranan Pattano ,&nbsp;Rosesathorn Soontarach ,&nbsp;Supayang Piyawan Voravuthikunchai ,&nbsp;Supakit Paosen","doi":"10.1016/j.tvjl.2025.106426","DOIUrl":"10.1016/j.tvjl.2025.106426","url":null,"abstract":"<div><div>Multidrug-resistant avian pathogenic <em>Escherichia coli</em> (MDR-APEC) causes high mortality in newborn chickens, leading to significant economic losses for poultry industry worldwide. The present study proposes an effective alternative strategy to control APEC infections by using bacteriophage specific to MDR-APEC. Lytic phage CABI-SEA 2301 isolated from a chicken faecal sample collected at a commercial poultry farm using the double layer agar overlay technique was classified as a novel species in the genus <em>Seuratvirus</em>, subfamily Queuovirinae. Genome sequencing unveiled that the selected phage consisted of a double-stranded DNA molecule with 61,452 base pairs and a GC content of 38 %, with no toxins, virulence, and antimicrobial resistance. Phage CABI-SEA 2301 demonstrated a broad host range, lysing 68 % of 50 MDR-APEC clinical isolates obtained from <em>E. coli</em> lesions at poultry farms. A high adsorption rate to the host was exhibited with 77 % within 10 min. One-step growth analysis showed that phage CABI-SEA 2301 had a latent period of 30 min with a burst size of 522 PFU/cell. Phage-infected MDR-APEC isolate CABI-SEA 2349 at the lowest multiplicity of infection of 0.01 showed a significant decrease in the bacterial growth after 4 h post-infection. Scanning electron microscopy further confirmed abnormal cellular morphology after treatment. Moreover, phage CABI-SEA 2301 could effectively inhibit the bacterial biofilm formation. Interestingly, the phage survived in mimic simulated chicken gastrointestinal conditions (96.67 ± 4.71 %) and revealed good stability under temperature between −80–50 ˚C and pH 4–12. The findings highlight the potential of the phage CABI-SEA 2301 as a biocontrol agent against MDR-APEC in the commercial farms.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"314 ","pages":"Article 106426"},"PeriodicalIF":3.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The predictive ability of blood-based biomarkers to detect bacteremia in hospitalized neonatal foals","authors":"Amanda N. Samuels ,&nbsp;Niamh M. Collins ,&nbsp;Kelly Hanlon ,&nbsp;Celine Bartish ,&nbsp;Payton Kelly ,&nbsp;Ahmed M. Kamr ,&nbsp;Ramiro E. Toribio","doi":"10.1016/j.tvjl.2025.106427","DOIUrl":"10.1016/j.tvjl.2025.106427","url":null,"abstract":"<div><div>Early and accurate identification of septicemia in neonatal foals improves survival. In human medicine, the neutrophil-to-lymphocyte ratio (NLR), neutrophil-to-monocyte ratio (NMR), monocyte-to-lymphocyte ratio (MLR), and plasma cell-free DNA (cfDNA) aid in early bacteremia detection. This study evaluated the diagnostic utility of these markers in conjunction with other clinical and hematological parameters in hospitalized foals &lt; 5 days old to predict positive blood culture at admission and to distinguish between Gram-positive, Gram-negative, or polymicrobial bacteremia. A total of 391 foals with a complete blood count and aseptically obtained blood culture at admission were included. Physical exam and hematologic parameters, including white blood cell count (WBC) and immunoglobulin G (IgG), were incorporated into logistic regression models, with the area under the curve (AUC) used to assess predictive performance. Plasma cfDNA was measured via fluorometry. We found that plasma cfDNA, IgG, NLR, and WBC were independent predictors of bacteremia, and a composite model demonstrated excellent discriminatory ability to identify foals with a positive blood culture (AUC = 0.806). Additionally, plasma cfDNA, IgG, and neutrophil counts were independent predictors of Gram-negative bacteremia, and a composite model demonstrated excellent discrimination (AUC = 0.807), and monocyte count and age predicted Gram-positive bacteremia with a composite model that demonstrated fair discriminatory ability (AUC = 0.67). Our findings demonstrate that NLR and plasma cfDNA are significantly altered in bacteremic foals. whereas the NMR and MLR do not differ significantly between groups. Combining these markers with other clinicopathologic variables may enable early identification and timely intervention in affected foals.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"314 ","pages":"Article 106427"},"PeriodicalIF":3.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference intervals for blood biochemical parameters in growing male water buffaloes (Bubalus bubalis)","authors":"Camila Sabino de Oliveira ,&nbsp;Caroline de Lima Francisco ,&nbsp;Daiane Cristina Marques da Silva ,&nbsp;André Michel de Castilhos ,&nbsp;Eunice Oba ,&nbsp;André Mendes Jorge","doi":"10.1016/j.tvjl.2025.106425","DOIUrl":"10.1016/j.tvjl.2025.106425","url":null,"abstract":"<div><div>Reference intervals for blood variables in domestic animals are essential to diagnosing diseases and identifying physiological abnormalities in animal production. Establishing such intervals for water buffaloes is important due to the growing use of this species in livestock, their relevance as a major source of meat and milk, and the lack of specific reference data for certain categories within the species. This study evaluated the blood parameters in growing male water buffaloes. Seventy-five animals from three genetic groups (Jafarabadi, Mediterranean, and Murrah), with an initial body weight of 309.1 ± 60.6 kg and age of 13 ± 1.2 months, were used. All animals were clinically healthy and blood samples were collected from their jugular vein on days 0, 56, and 84. The concentrations of total protein, high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL), albumin, aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), creatinine, bilirubin (total, indirect, and direct), total cholesterol, glucose, lipoproteins, triglycerides, and leptin were analysed using commercial kits. Reference intervals, 90 % confidence intervals, means, standard deviations, and reference values were calculated according to the guidelines of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) by excluding the upper and lower 2.5 % of values for each biochemical parameter. Sixteen blood biochemical parameters were characterised for growing male water buffaloes. The findings provide valuable data for interpreting health status and supporting management practices in water buffalo meat production systems.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"314 ","pages":"Article 106425"},"PeriodicalIF":3.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lycopene exerts a treatment effect on bovine mastitis induced by Escherichia coli infection via the MAPK pathway using network pharmacology, a mice model and molecular docking","authors":"Tianyu Yang,&nbsp;Jiangang Bai,&nbsp;Yucan Wu,&nbsp;Cuicui Zhuang","doi":"10.1016/j.tvjl.2025.106428","DOIUrl":"10.1016/j.tvjl.2025.106428","url":null,"abstract":"<div><div><em>Escherichia coli</em>, an opportunistic pathogen, induces acute bovine mastitis, leading to severe milk yield reduction, dairy product discard, and substantial economic losses. This not only jeopardizes cow health and farm profitability but also raises food safety concerns, highlighting the need for effective treatments<em>.</em> Lycopene (LYC), a natural carotenoid with potent anti-inflammatory and antioxidant properties, has shown potential in mitigating inflammatory diseases. This study investigated the therapeutic mechanism of LYC against <em>E. coli</em>-induced bovine mastitis using network pharmacology, molecular docking, and a mice model. Potential targets of LYC were screened from TargetNet, GeneCards, CTD and SwissTargetPrediction, while mastitis and <em>E. coli</em> infections-related genes were obtained from GeneCards and CTD, respectively. PPI networks were constructed via STRING and Cytoscape, and core targets were analyzed using GO and KEGG in Metascape database. Molecular docking was performed to validate binding affinities between LYC and key targets. An <em>E. coli</em>-induced mastitis mice model was established to evaluate LYC's efficacy, with inflammatory markers and MAPK pathway proteins assessed via ELISA and Western blot. Network pharmacology identified 165 overlapping targets among LYC, mastitis and <em>E. coli</em> infections. KEGG analysis highlighted the MAPK pathway as a critical signalling axis, with 15 core regulators. Molecular docking confirmed strong binding of LYC to these core regulators. <em>In vivo</em> experiments demonstrated that LYC significantly reduced mammary tissue damage, decreased pro-inflammatory cytokines, and suppressed MAPK pathway in <em>E. coli</em>-infected mice. Therefore, LYC alleviates <em>E. coli</em> infection-induced bovine mastitis by modulating the MAPK pathway, offering a promising natural alternative for mastitis treatment.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"314 ","pages":"Article 106428"},"PeriodicalIF":3.1,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of canine gallbladder cholangiocyte organoids from healthy and gallbladder mucocele patient dogs for functional analysis of anion channels","authors":"Itsuma Nagao ,&nbsp;Hirotaka Tomiyasu ,&nbsp;Yuko Goto-Koshino ,&nbsp;Kota Nakase ,&nbsp;Takumi Tsuruta ,&nbsp;Yuto Namiki ,&nbsp;Ryuichiro Sato ,&nbsp;Yu Takahashi ,&nbsp;Yuko Hashimoto ,&nbsp;Yosuke Takahashi ,&nbsp;Naohiro Takahashi ,&nbsp;James K. Chambers ,&nbsp;Kazuyuki Uchida ,&nbsp;Kenji Baba ,&nbsp;Yoko M. Ambrosini ,&nbsp;Masaru Okuda","doi":"10.1016/j.tvjl.2025.106417","DOIUrl":"10.1016/j.tvjl.2025.106417","url":null,"abstract":"<div><div>Gallbladder mucocele (GBM) is one of the most common gallbladder disorders in dogs. Recent studies have suggested a potential association between GBM development and reduced expression of anion channel-related molecules in gallbladder epithelial cells. However, further investigation has been limited due to the lack of an effective <em>in vitro</em> culture system to validate the functions of the molecules. This study aimed to establish gallbladder cholangiocyte organoids (GCOs) from canine gallbladders and evaluate anion channel functions. GCOs were generated from gallbladders of three clinically healthy dogs and three GBM-affected dogs, followed by morphological evaluation, comparisons of anion channel-related gene expressions, and functional assessment of anion channels, specifically cystic fibrosis transmembrane conductance regulator (CFTR) and calcium-activated chloride channels (CaCCs), using organoid swelling assays. Most GCOs derived from normal gallbladder (N-GCOs) exhibited a round, cystic morphology, whereas GCOs from GBM-affected gallbladder (GBM-GCOs) predominantly displayed a budding morphology with narrowed lumen. Although no significant differences in anion channel-related gene expression were observed between the two groups, the functions of both CFTR and CaCCs were significantly impaired in GBM-GCOs. The morphological differences between the groups might be attributed to the reduced ion secretions and subsequent shortages of water transport into the organoid lumen in GBM-GCOs, leading to insufficient lumen expansion. These findings highlighted a potential link between anion channel dysfunction and GBM pathogenesis. Further studies utilizing canine GCOs are warranted to deepen our understanding of the role of anion channel dysfunction in GBM development.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106417"},"PeriodicalIF":3.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When should we start to treat feline CKD: A narrative review of early diagnosis and the evidence for pre-azotaemic intervention","authors":"Jason P. Bestwick,&nbsp;Rebecca F. Geddes","doi":"10.1016/j.tvjl.2025.106416","DOIUrl":"10.1016/j.tvjl.2025.106416","url":null,"abstract":"<div><div>Chronic kidney disease (CKD) is a common and important disease in ageing cats. Increased awareness and availability of a novel biomarker for detecting glomerular filtration rate reduction (symmetric dimethylarginine), alongside efforts by the International Renal Interest Society to standardise diagnosis and staging, have led to earlier detection of CKD and an ability to study and trial interventions in sub-populations based on disease severity. As weight loss and phosphorus dyshomeostasis are present in non-azotaemic cats, and progression to azotaemic CKD may occur over time, earlier diagnosis and management represent a compelling opportunity for optimising management of this disease. To date, however, dietary modification (particularly protein- and phosphorus-restriction) in cats with azotaemic CKD is the only intervention demonstrating robust evidence for improving survival times and slowing disease progression. The purpose of this article is to review the available literature supporting criteria currently used to diagnose non-azotaemic CKD and evidence for early intervention, with a focus on dietary modification.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106416"},"PeriodicalIF":3.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144790134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics of mastitis in Gir cows: A comprehensive thermographic assessment","authors":"S.L. Gayathri ,&nbsp;M. Bhakat ,&nbsp;T.K. Mohanty","doi":"10.1016/j.tvjl.2025.106400","DOIUrl":"10.1016/j.tvjl.2025.106400","url":null,"abstract":"<div><div>Mastitis is a pervasive inflammatory disease of the mammary gland. It poses substantial challenges to global dairy farming by reducing milk quality, impacting animal welfare, and incurring substantial economic losses. This study hypothesized that infrared thermography (IRT) could serve as a non-invasive diagnostic tool for detecting sub-clinical (SCM) and clinical mastitis (CM) in <em>Bos indicus</em> (Gir) cows across diverse seasonal conditions. Thermographic parameters, including udder and short-milking-tube (SMT) temperatures, were evaluated in conjunction with the California Mastitis Test (CMT) and somatic cell count (SCC). Receiver operating characteristic (ROC) analysis assessed the diagnostic performance of IRT, and Pearson's correlation coefficients were computed to explore relationships between thermal metrics, SCC, and CMT scores. The findings revealed temperature differences (p &lt; 0.01) in the eyeball, SMT, and udder skin surface across pre-milking, milking, and post-milking phases, with notable seasonal variations among healthy, SCM, and CM quarters. ROC analysis demonstrated higher sensitivity, specificity, and accuracy for CM cases than SCM across all thermographic parameters. These findings confirmed that IRT effectively captured seasonal and lactation phase-specific variations in udder and teat temperatures, along with SMT temperature variations during the milking phase. These results underscore IRT’s efficacy in detecting inflammation-induced thermal variations, offering a reliable, non-invasive approach for early mastitis diagnosis. This study highlights the potential of IRT to transform mastitis detection in <em>Bos indicus</em> breeds by enabling timely interventions and enhancing herd management strategies. Its integration into precision livestock farming aligns with global efforts to promote sustainable dairy production and improve animal welfare.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106400"},"PeriodicalIF":2.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144614705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"1H NMR metabonomics and immune cell signature of milk may reveal insights into subclinical mastitis and quarter interdependence","authors":"Valentina Bonfatti ,&nbsp;Federico Bonsembiante ,&nbsp;Elisa Giaretta ,&nbsp;Paola Vanzani ,&nbsp;Maria Elena Gelain ,&nbsp;Alfonso Zecconi ,&nbsp;Lucio Zennaro ,&nbsp;Gianfranco Gabai ,&nbsp;Fabio Vianello","doi":"10.1016/j.tvjl.2025.106401","DOIUrl":"10.1016/j.tvjl.2025.106401","url":null,"abstract":"<div><div>Milk metabolome depends on a plethora of factors and on the presence of different cell types and could help understanding the biology of the mammary gland and, possibly, identifying biomarkers for mastitis, tissue repairing and milk quality. To fulfill these expectations, metabolome changes need an accurate characterization under several well-characterized physiological and pathophysiological conditions. The aim of the present work is to study mammary quarters of dairy cows affected by subclinical mastitis (SCM) and acute inflammation compared to healthy animals. The milk metabolome was investigated by ¹H NMR spectroscopy and by the assessment of somatic cell populations by flow cytometry using a panel of leukocyte markers (CD11b, CD44, CD14, CD4, CD8, CD21). The study was integrated by microbiological evaluations and oxidized proteins (AOPP) determination and results were analyzed by multivariate model. Mammary quarters with the highest CD11b positive cells, suggestive of acute inflammation, were present in SCM-affected cows only, and were characterized by significantly higher AOPP, where the microbiological analysis revealed the presence of minor pathogens. A good PCA separation between healthy and SCM-infected animals was observed (overall error rate: 0.177 ± 0.056) confirming that SCC are associated with modifications of milk metabolome. The classification accuracy was lower (overall error rate: 0.343 ± 0.029) when the mammary quarters were classified on the fraction of CD11b positive cells of quarters from healthy and SCM-affected cows. Interestingly, low-CD11b-SCM samples tended to be misclassified (error rate: 0.460), suggesting the influence of infected neighboring quarters. The results of this work underlay the importance of studying the functional interdependence of mammary quarters in animals affected by SCM.</div></div><div><h3>Simple summary</h3><div>This study explored how subclinical mastitis—a mild, often hidden udder infection in dairy cows—affects the metabolites in milk. Nuclear magnetic resonance spectroscopy was used for comparing milk from healthy cows to that produced by cows with subclinical mastitis caused by bacterial infections. We found that the metabolic profile (metabolome) of analyzed milk changed noticeably in infected cows. These changes were linked to both immune cell activity and possible damage to udder tissue. Surprisingly, even parts of the udder that seemed healthy in infected cows sometimes showed altered milk composition. This suggests that infections in one part of the udder can influence nearby quarters or cause broader changes in the cow’s immune and metabolic systems. The findings support the suggestion that milk metabolites could be used to detect early stages of udder infections. The study also highlights how different quarters of the udder are connected, and how even the “healthy” ones can be affected when a cow suffers from mastitis.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106401"},"PeriodicalIF":2.3,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Slow kill\" treatment protocol in naturally infected dogs with Dirofilaria immitis and assessment of serum NT-proBNP and plasma D-dimer as biomarkers of this infection","authors":"Milan Rajković ,&nbsp;Uroš Glavinić ,&nbsp;Danica Bogunović ,&nbsp;Saša Trailović ,&nbsp;Jevrosima Stevanović ,&nbsp;Goran Cvetković ,&nbsp;Zoran Stanimirović","doi":"10.1016/j.tvjl.2025.106399","DOIUrl":"10.1016/j.tvjl.2025.106399","url":null,"abstract":"<div><div>This study assessed the efficacy of the \"slow kill\" protocol (ivermectin and doxycycline) on <em>Dirofilaria immitis</em> infection in dogs and the biomarker value of NT-proBNP and D-dimer in these cases. Dogs were tested for heartworm presence using a rapid antigen test, and blood samples were collected on days: 0 (<em>T</em>_<em>0</em>), 90 (<em>T</em>_<em>90</em>), and 180 (<em>T</em>_<em>180</em>). Dogs were divided into three groups based on clinical symptoms: asymptomatic (<em>G</em>_<em>ASX</em>), moderate (<em>G</em>_{<em>MODERATE</em>}), and severe (<em>G</em>_{<em>SEVERE</em>}). At <em>T</em>_<em>0</em>, NT-proBNP levels were within the reference values in <em>G</em>_<em>ASX</em> and <em>G</em>_{<em>MODERATE</em>} groups but elevated in <em>G</em>_{<em>SEVERE</em>} and significantly (p &lt; 0.05) higher than in <em>G</em>_<em>ASX</em>. D-dimer levels were within the reference range in all groups without significant (p &gt; 0.05) differences among them. At <em>T</em>_<em>90</em>, NT-proBNP remained within the reference values only in <em>G</em>_<em>ASX</em> and was not significantly different (p &gt; 0.05) among groups. However, D-dimer levels increased in <em>G</em>_{<em>MODERATE</em>} and <em>G</em>_{<em>SEVERE</em>,} being significantly (p &lt; 0.05) higher than in <em>G</em>_<em>ASX</em> group. At <em>T</em>_<em>180</em>, NT-proBNP was elevated in all groups, being significantly higher in <em>G</em>_{<em>MODERATE</em>} (p &lt; 0.05) and <em>G</em>_{<em>SEVERE</em>} (p &lt; 0.01) compared to <em>G</em>_<em>ASX</em>. D-dimer decreased across all groups but remained above the reference values, being significantly higher in <em>G</em>_{<em>MODERATE</em>} (p &lt; 0.05) and <em>G</em>_{<em>SEVERE</em>} (p &lt; 0.001) than in <em>G</em>_<em>ASX</em>. The \"slow kill\" treatment did not reduce NT-proBNP and D-dimer levels, having in mind long retention of adults, suggesting that cardiac and antithrombotic therapies may be necessary. Considering the relationship between the severity of clinical pictures and the levels of NT-proBNP and D-dimer, they have potential as prognostic markers in veterinary practice for heartworm-infected dogs.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106399"},"PeriodicalIF":2.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interference effect of foot-and-mouth disease maternal antibodies following foot-and-mouth disease vaccination and its related factors in Hanwoo beef calves","authors":"Ui-Hyung Kim ,&nbsp;Sung-Sik Kang ,&nbsp;Myung-Sun Park ,&nbsp;Seungmin Ha","doi":"10.1016/j.tvjl.2025.106397","DOIUrl":"10.1016/j.tvjl.2025.106397","url":null,"abstract":"<div><div>Foot-and-mouth disease (FMD) vaccinations have been conducted in Korea for &gt; 10 years. However, few studies have confirmed the optimal timing of FMD vaccination in newborn calves. In this study, we aimed to evaluate changes in antibodies before and after FMD vaccination and identify potential factors affecting FMD maternal-derived antibodies in newborn calves. Calves were vaccinated at 8 and 12 weeks of age. Blood samples were obtained from dams 6 weeks before the estimated due date and from calves at 8 (pre-vaccination), 12, and 16 weeks of age. Antibodies against the FMD virus (FMDV) structural proteins were detected using Priocheck FMDV type O antibody enzyme-linked immunosorbent assay. Among 82 calves, 54 (65.9 %) exhibited a decrease in FMD percentage inhibition (PI) at 16 weeks. The mean pre-vaccination FMD PI values of calves whose FMD PI decreased and increased after vaccination were 92.4 ± 9.4 and 72.4 ± 16.9, respectively (<em>p</em> &lt; 0.05). Our results indicate that FMD vaccination at 8 weeks is early due to the high levels of maternal-derived FMD antibodies in the body of Hanwoo calves. The FMD PI of the dam (β = 0.636) exhibited the most significant impact on the FMD PI of 8-week-old calves, followed by calf birth weight (β = 0.200) (<em>p</em> &lt; 0.05). Calves born to dams with high FMD PI and heavy birth weight exhibited high FMD PI at 8 weeks.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"313 ","pages":"Article 106397"},"PeriodicalIF":2.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}