Veterinary journal最新文献_第5页

Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study 作为马肥胖研究工具的脂肪细胞的分离方法和特性-体外研究

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-07 DOI: 10.1016/j.tvjl.2025.106354

Paweł Antoni Kołodziejski , Natalia Leciejewska , Maciej Sassek , Leszek Nogowski , Małgorzata Szumacher-Strabel , Robert Mikuła , Maciej Gogulski , Ewa Pruszyńska-Oszmałek

{"title":"Isolation method and characterization of adipocytes as a tool for equine obesity research – In vitro study","authors":"Paweł Antoni Kołodziejski , Natalia Leciejewska , Maciej Sassek , Leszek Nogowski , Małgorzata Szumacher-Strabel , Robert Mikuła , Maciej Gogulski , Ewa Pruszyńska-Oszmałek","doi":"10.1016/j.tvjl.2025.106354","DOIUrl":"10.1016/j.tvjl.2025.106354","url":null,"abstract":"<div><div>Adipose tissue functions as an endocrine organ; however, excessive lipid accumulation can lead to obesity and metabolic disorders, such as Equine Metabolic Syndrome (EMS), characterized by insulin resistance, fat deposition, and increased inflammation. Despite the growing prevalence of obesity in horses, knowledge of equine adipocytes and their metabolic functions remains limited. The main objective of the study was to develop and optimize a method for isolating equine adipocytes and to characterize their metabolic activity. Using slaughterhouse-derived horse visceral adipose tissue, we developed a protocol to isolate mature adipocytes. Metabolic activity of cells was assessed by examining their sensitivity to lipolytic factors: isoproterenol (0.001–10 µM), epinephrine (0.001–1 µM), and forskolin (0.001–1 µM)—and lipogenesis intensity after stimulation with insulin. We obtained mature equine adipocytes with diameters ranging from 50 to 160 µm. These cells demonstrated full metabolic functionality, responding to lipolytic factors such as isoproterenol (all doses: p < 0.001), epinephrine (0.01 µM: p < 0.05; 0.1–1 µM: p < 0.0001), and forskolin (0.001 µM: p < 0.0001). The adipocytes also responded to insulin from all tested species, with effects being dose- and time-dependent (after 2 h human insulin 10 nM, p < 0.05; bovine 10, 100 nM p < 0.05 and after 8 h all doses p < 0.05). The presented method for isolating mature equine adipocytes is effective, yielding metabolically functional cells, which can serve as a valuable in vitro model for studying the effects of various factors on adipocyte function, contributing to a better understanding of equine adipose tissue dysfunction, particularly in the context of metabolic disorders.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106354"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A systematic review of the use of peri-operative systemic ketamine in cats and dogs for analgesia 猫狗围手术期全身性氯胺酮镇痛的系统回顾

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-07 DOI: 10.1016/j.tvjl.2025.106350

Francesca Wickstead , Miguel Martinez

{"title":"A systematic review of the use of peri-operative systemic ketamine in cats and dogs for analgesia","authors":"Francesca Wickstead , Miguel Martinez","doi":"10.1016/j.tvjl.2025.106350","DOIUrl":"10.1016/j.tvjl.2025.106350","url":null,"abstract":"<div><h3>Objectives</h3><div>Ketamine is a non-competitive N-methyl-D-aspartate antagonist widely used in veterinary medicine for analgesia peri-operatively, in polytrauma and anecdotally for chronic pain. This systematic review aimed to provide an overview of the veterinary literature available on systemic ketamine administration for acute analgesia in dogs and cats. This will include, a discussion on ketamine’s impact on pain scores and rescue analgesia requirements and will correlate these with ketamine plasma concentrations and nociceptive threshold changes.</div></div><div><h3>Methods</h3><div>A literature search was conducted over three databases (GoogleScholar, PubMed and Veterinary Anaesthesia and Analgesia Journal) with definitive search terms and inclusion criteria between 1980 and 2024. Quality of evidence was assessed with the ‘Grading of Recommendations, Assessment, Development, and Evaluations’ and the Cochrane Collaboration Bias tool to assess study bias.</div></div><div><h3>Results</h3><div>Studies that met eligibility criteria included 11 dogs, three cats; 14 in total. These included prospective randomised controlled trials, crossover trials, case series and laboratory experiments. Quality of evidence was moderate, with low risk of bias, moderate-good study design, moderate indirectness due to predominantly soft tissue procedures studied and underpowered, small population studies.</div></div><div><h3>Conclusion</h3><div>Ketamine may influence pain scores > 12 hours post operatively. Ketamine does not influence rescue analgesia requirements post-operatively. Plasma concentrations > 200 ng ml<sup>−1</sup> correlate with nociceptive threshold changes. When doses are extrapolated from humans, plasma concentrations are lower than expected. Plasma concentrations quickly decline after cessation of intravenous infusion or bolus. Future studies with higher doses of ketamine in a variety of clinical procedures are needed to evaluate ketamine's analgesic effect peri-operatively.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"312 ","pages":"Article 106350"},"PeriodicalIF":2.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the performance of VP1 expressed in baculovirus and Escherichia coli expressed from Senecavirus A in pig using an ELISA 用酶联免疫吸附法评价猪赛尼加病毒A型杆状病毒和大肠埃希菌表达VP1的性能。

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106346

Parin Watcharavongtip , Patumporn Jermsutjarit , Angkana Tantituvanont , Dachrit Nilubol

引用次数: 0

ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance 常青复方通过调节肠道益生菌和致病菌平衡缓解柔嫩艾美耳球虫感染症状。

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106343

Jiajia Yang , Xuan Wang , Ying Chen , Ye He , Ping Li , Xin Wen , Bi Wang

{"title":"ChangQing compound relieves Eimeria tenella infection symptoms by modulating intestinal probiotic and pathogenic bacteria balance","authors":"Jiajia Yang , Xuan Wang , Ying Chen , Ye He , Ping Li , Xin Wen , Bi Wang","doi":"10.1016/j.tvjl.2025.106343","DOIUrl":"10.1016/j.tvjl.2025.106343","url":null,"abstract":"<div><div>Cecal coccidiosis is a severe and lethal parasitic disease affecting chickens, making the search for effective preventive agents free of contamination and drug resistance crucial for controlling this condition in poultry. Previous studies have demonstrated that the ChangQing compound has significant therapeutic effects against cecal coccidiosis; however, its potential as a preventive measure has yet to be evaluated. In this study, we established an experimental model for the prevention of cecal coccidiosis in chickens using the ChangQing compound for the first time. A comprehensive evaluation was performed on survival rates, relative weight gain, oocyst production, the anticoccidial index (ACI), immune parameters, parasitic tissue pathology, and microbial diversity in cecal contents. Results indicated that the ChangQing compound at a concentration of 5.0 g/L achieved an ACI of 178.10 in the Pre-Exposure Prophylaxis (PrEP) group and 173.12 in the Post-Exposure Prophylaxis (PEP) group. Compared to the positive control group, cecal lesions were reduced, and indices for the spleen, liver, and bursa of Fabricius increased in both experimental groups following ChangQing administration. Furthermore, levels of immune factors, IgA, IgG, and IgM significantly elevated. The abundance of beneficial bacteria, including <em>Lactobacillus</em>, <em>Bacteroides</em>, and <em>Alistipes</em>, increased in the 5.0 g/L ChangQing compound group, while potential pathogens like <em>Escherichia-Shigella</em>, <em>Enterococcus</em>, and <em>norank_f_Oscillospiraceae</em> were reduced. These findings offer critical data for coccidiosis prevention in chickens and lay a theoretical foundation for future research on the antiparasitic mechanisms of traditional Chinese medicine.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106343"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diode Laser Epiglottidectomy (DLE) for management of epiglottic disease in 35 dogs 二极管激光会厌瓣切除术治疗会厌疾病35例。

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106345

Davide De Lorenzi , Chiara Mantovani , Giulia Maggi , Enrico Bottero , Maria C. Marchesi

{"title":"Diode Laser Epiglottidectomy (DLE) for management of epiglottic disease in 35 dogs","authors":"Davide De Lorenzi , Chiara Mantovani , Giulia Maggi , Enrico Bottero , Maria C. Marchesi","doi":"10.1016/j.tvjl.2025.106345","DOIUrl":"10.1016/j.tvjl.2025.106345","url":null,"abstract":"<div><div>Veterinary literature on epiglottic disorders and their treatment in dogs is limited. The objective of this study is to report the clinical features, diagnosis, surgical treatment, and outcomes of dogs with epiglottic conditions treated using diode laser epiglottidectomy (DLE). This was a single-institution observational prospective study. Thirty-five dogs diagnosed with epiglottic disease underwent DLE. In all cases, an epiglottic disease was documented with combined laryngoscopic examination, when needed, fluoroscopy, computed tomography (CT) scan, and biopsy. Sub-total (SDLE) and total (TDLE) diode laser epiglottidectomy was performed under endoscopic guidance according to the diagnosis. Follow-up was performed by a re-examination visit and endoscopy, and telephone follow-up with the owner. The most common epiglottic disorder was epiglottic retroversion (ER) (57.1 %). SDLE was performed in 32/35 (91.4 %) dogs, while 3/35 (8.6 %) dogs underwent TDLE. Intraoperative complications occurred in 11.4 % surgeries, primarily involving bleeding. Postsurgical complications were reported in 8.5 % cases post-SDLE and were represented by transient airway obstruction caused by local edema. Follow-up (median 18 months, minimum 3 months – maximum 21 months) consultations revealed prolonged resolution of upper airway obstruction without signs of respiratory tract compromise or dysphagia. The surgical techniques described in this study have proven to be effective and minimally invasive for the treatment of epiglottic-related airway obstruction.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106345"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of mentorship in veterinary practice: An empirical study of the Animal Health Mentoring Framework in Pakistan 指导在兽医实践中的作用：巴基斯坦动物健康指导框架的实证研究。

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-03 DOI: 10.1016/j.tvjl.2025.106348

Ujala Fatima Shan , Aneela Zameer Durrani , Muhammad Zahid Iqbal , Shakera Sadiq Gill , Esha Binte Iqbal , Sadia Sanaullah , Muhammad Aftab , Mahrukh Ahmed , Safdar Aman

{"title":"The role of mentorship in veterinary practice: An empirical study of the Animal Health Mentoring Framework in Pakistan","authors":"Ujala Fatima Shan , Aneela Zameer Durrani , Muhammad Zahid Iqbal , Shakera Sadiq Gill , Esha Binte Iqbal , Sadia Sanaullah , Muhammad Aftab , Mahrukh Ahmed , Safdar Aman","doi":"10.1016/j.tvjl.2025.106348","DOIUrl":"10.1016/j.tvjl.2025.106348","url":null,"abstract":"<div><div>This research introduced and ascertained the effectiveness of the Animal Health Mentoring Framework (AHMF) designed by Brooke, a global welfare organization, to develop the dual skills of ethical reasoning and the technical capabilities of field veterinarians in Pakistan. A longitudinal observational study was carried out on 100 veterinary professionals from six institutions in Lahore for six months by engaging them in a structured mentorship program. The AHMF targeted key developmental areas: welfare advocacy, clinical expertise, communication, kit content and maintenance, and clinical governance. Monthly assessments were carried out using the AHMF scoring tool, and the results were analyzed using the Friedman test across these five competencies. The results showed statistically significant improvements in all aspects. The welfare advocacy score increased from 1.13 at the start to 3.62 by the end of the study, clinical expertise improved from 8.26 to 12.51 while clinical governance enhanced from 0.14 to 1.12. Communication skills and kit management also improved significantly, although at a slightly slower rate. The average total AHMF score of the participants increased from 13.14 to 22.37, thus emphasizing all-rounded improvements in veterinary practices. The outcomes suggest that formal mentoring through the AHMF significantly enhances veterinary professionals' ethical and technical competencies, particularly in resource-poor settings. AHMF is a scalable, globally adaptable model, that can improve veterinary education, practice, and animal welfare, particularly in low and middle-income countries. This pioneering research sets a benchmark for professional development and offers a practical solution to elevate animal welfare and clinical expertise globally.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106348"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143789041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasibility and cartilage injury of an all-inside arthroscopic meniscal repair application in a canine cadaveric study 全内关节镜半月板修复术在犬尸体研究中的可行性及软骨损伤

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-02 DOI: 10.1016/j.tvjl.2025.106339

David Michalik , Robert A. Pettitt , Alexander Tichy , Eva Schnabl-Feichter

{"title":"Feasibility and cartilage injury of an all-inside arthroscopic meniscal repair application in a canine cadaveric study","authors":"David Michalik , Robert A. Pettitt , Alexander Tichy , Eva Schnabl-Feichter","doi":"10.1016/j.tvjl.2025.106339","DOIUrl":"10.1016/j.tvjl.2025.106339","url":null,"abstract":"<div><div>Meniscal injury is the most common comorbidity in canine stifles with cranial cruciate ligament (CCL) pathology. Arthroscopic repair for acute tears can be technically challenging and is rarely described. This study evaluates the feasibility of using an all-inside arthroscopic meniscal repair device, Arthrex Meniscal Cinch II™ (MCII) in canine cadaveric stifles and the associated risks of iatrogenic vascular injury (IVI) and cartilage injury (ICI). 20 healthy paired canine cadaveric stifles (25 – 45 kg BW) were divided randomly into two groups of 10. Both groups received transection of the CCL via mini arthrotomy, joint distraction and diagnostic arthroscopy with meniscal probing. No further procedures were performed in the control group whereas the implant group underwent placement of the MCII-implants in the caudal horn of the medial meniscus. Angiography of the femoral artery was performed for each limb before and after operation and vascular trauma was assessed on radiographs. After disarticulation, the cartilage was stained via Indian ink assay and underwent blinded scoring for ICI. Implants were evaluated for desired position. Correct position of the MCII-implants was achieved in all stifles. No IVI was detected. Implant placement created more ICI on the medial femoral condyle (1.33 mm<sup>2</sup>) than arthroscopy alone (0.15 mm<sup>2</sup>) (p = 0.03). Implant associated complications occurred in 30 %. Arthroscopic use of MCII is feasible and carries minimal risk for vascular damage. Despite joint distraction, implant placement using the MCII is associated with significant cartilage damage. Although technically challenging, all-inside meniscal repair appears to carry limited risk in canine patients over 25 kg bodyweight.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106339"},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic evaluation of meloxicam following intravenous and intramuscular administration in Crocodylus siamensis, a freshwater crocodile 淡水鳄鱼siamensis静脉注射和肌肉注射美洛昔康的药代动力学评价

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-04-01 DOI: 10.1016/j.tvjl.2025.106342

Seavchou Laut , Saranya Poapolathep , Kraisiri Khidkhan , Narumol Klangkaew , Napassorn Phaochoosak , Tara Wongwaipairoj , Mario Giorgi , Pedro Marin , Elisa Escudero , Amnart Poapolathep

{"title":"Pharmacokinetic evaluation of meloxicam following intravenous and intramuscular administration in Crocodylus siamensis, a freshwater crocodile","authors":"Seavchou Laut , Saranya Poapolathep , Kraisiri Khidkhan , Narumol Klangkaew , Napassorn Phaochoosak , Tara Wongwaipairoj , Mario Giorgi , Pedro Marin , Elisa Escudero , Amnart Poapolathep","doi":"10.1016/j.tvjl.2025.106342","DOIUrl":"10.1016/j.tvjl.2025.106342","url":null,"abstract":"<div><div>The pharmacokinetics of meloxicam (MLX) remain largely unexplored in reptiles, particularly in Siamese crocodiles (<em>Crocodylus siamensis</em>). This study characterized the pharmacokinetic profiles of MLX following intravenous (IV) and intramuscular (IM) administration in Siamese crocodiles. Fifteen Siamese crocodiles were divided into three groups (n=5) using a randomization procedure according to a parallel study design. MLX was administered IV at 0.2mg/kg b.w. or IM at two different doses (0.2mg/kg b.w. or 0.4mg/kg b.w.). Plasma concentrations of MLX were measured using a validated high-performance liquid chromatography method with UV detection. The pharmacokinetic parameters were analyzed using a non-compartment model. The elimination half-life (t<sub>1/2λz</sub>) was long for all administration routes, with values of 132.34hr (IV), 121.35hr (IM 0.2mg/kg b.w.), and 181.44hr (IM 0.4mg/kg b.w.). The volumes of distribution (Vd) and clearance (Cl) after IV administration were 104.59mL/kg and 0.55mL/hr/kg, respectively. Based on these results, there was an extended t<sub>1/2λz</sub> of MLX in this species of freshwater crocodiles, highlighting significant differences in drug disposition compared to other reptilian and non-reptilian species. The findings contribute to an understanding of MLX pharmacokinetics in this animal species, and emphasize that the selection of the optimal dose of MLX should be considered based on disposition kinetics, efficacy, safety, and species-specific differences. Further investigation is required to identify the effective plasma concentration, which is critical for establishing the appropriate dose for the management of pain and inflammation.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106342"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-chain fatty acids alleviate lung damage caused by interleukin-17 in contagious caprine pleuropneumonia. 短链脂肪酸减轻传染性羊胸膜肺炎白细胞介素-17所致肺损伤。

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-03-31 DOI: 10.1016/j.tvjl.2025.106320

Haoyue Yang, Yunpeng Wang, Yuecai Jiang, Silu Ni, Yiming Chen, Dekun Chen, Wenxiu Wang, Wentao Ma

{"title":"Short-chain fatty acids alleviate lung damage caused by interleukin-17 in contagious caprine pleuropneumonia.","authors":"Haoyue Yang, Yunpeng Wang, Yuecai Jiang, Silu Ni, Yiming Chen, Dekun Chen, Wenxiu Wang, Wentao Ma","doi":"10.1016/j.tvjl.2025.106320","DOIUrl":"https://doi.org/10.1016/j.tvjl.2025.106320","url":null,"abstract":"<p><p>Contagious caprine pleuropneumonia (CCPP) is a highly contagious and economically devastating disease in goats, caused by Mycoplasma capricolum subspecies capripneumoniae (Mccp). There is a limited understanding of the immunopathogenesis of this disease, which hinders the development of effective control strategies. Several researches have shown that short-chain fatty acids (SCFAs) have promising potential in the prevention and treatment of lung diseases. However, it remains unclear whether it produces similar therapeutic effects in the case of CCPP. Goat and mouse models were used in this study to investigate the mechanisms underlying the inflammatory immune response during CCPP infection. In mice, gut microbiota dysbiosis resulted in severe lung infection caused by Mccp. The damage to the lungs was significantly alleviated when SCFAs were preadministered to Mccp-infected mice. In the case of goat lung epithelial cells, pretreatment with SCFAs also significantly reduced the expression of the inflammatory cytokines IL-1β and TNF-α induced by MCCP infection and improved cell viability. The optimal therapeutic concentration of SCFAs was found to be 0.5 mg/mL. Our results demonstrate the potential of SCFA to prevent CCPP in goats by suppressing the inflammatory response.</p>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":" ","pages":"106320"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144664063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence of virulence genes and multi-drug resistance in Pasteurella multocida from goats in Sichuan, China 四川山羊多杀性巴氏杆菌毒力基因和多重耐药率高

IF 2.3 2区农林科学

Veterinary journal Pub Date : 2025-03-31 DOI: 10.1016/j.tvjl.2025.106344

Shenglin Li , Qibing Gu , Benrun Li, Kehamo Abi, Falong Yang

{"title":"High prevalence of virulence genes and multi-drug resistance in Pasteurella multocida from goats in Sichuan, China","authors":"Shenglin Li , Qibing Gu , Benrun Li, Kehamo Abi, Falong Yang","doi":"10.1016/j.tvjl.2025.106344","DOIUrl":"10.1016/j.tvjl.2025.106344","url":null,"abstract":"<div><div><em>Pasteurella multocida</em> is one of the most important pathogens that infect goats, causing serious economic losses in the goat breeding industry. To understand the biological characteristics of <em>P. multocida</em> from goats, a comprehensive characterization of bacteria isolated from 342 nasal swabs and 8 lung tissue samples from goat farms in Sichuan, China, was performed. A total of 34 isolates were assigned to one capsular type, D, and one lipopolysaccharide (LPS) genotype, L3, indicating that the D: L3 was the predominant serotype in goat farms. In the 34 isolates, multiple virulence-related genes were identified, with a detection rate of 100 % (34/34) for the genes <em>ompA</em>, <em>ompH</em>, <em>oma87</em>, <em>exbB</em>, and <em>exbD</em>. It is noteworthy that the prevalence of the <em>toxA</em> gene, which encodes the <em>P. multocida</em> toxin (PMT), was found to be 85.2 % (29/34). Furthermore, antimicrobial susceptibility testing indicated a high prevalence of multidrug resistance, with resistance rates of 41.1 % for ampicillin, 38.2 % for tetracycline, and 32.3 % for kanamycin. Overall, this study provides a foundational understanding of the epidemiology and antimicrobial resistance of <em>P. multocida</em> in goats, offering insights for future prevention and control measures.</div></div>","PeriodicalId":23505,"journal":{"name":"Veterinary journal","volume":"311 ","pages":"Article 106344"},"PeriodicalIF":2.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0