用酶联免疫吸附法评价猪赛尼加病毒A型杆状病毒和大肠埃希菌表达VP1的性能。

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

Veterinary journal Pub Date : 2025-04-03 DOI:10.1016/j.tvjl.2025.106346

Parin Watcharavongtip , Patumporn Jermsutjarit , Angkana Tantituvanont , Dachrit Nilubol

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引用次数: 0

摘要

塞内卡病毒A （SVA）引起猪特发性水疱病（PIVD），导致猪生产的经济损失。快速抗体检测对于监测群体免疫和控制疫情至关重要。本研究利用杆状病毒和大肠杆菌系统中表达的SVA VP1蛋白开发了间接elisa。优化后的elisa具有较高的灵敏度（杆状病毒为100%，大肠杆菌为96.67%）和特异性（均为96.67%），截止值为0.40。两者均与病毒中和试验（VNA）密切相关，且与其他猪病原体无交叉反应。基于杆状病毒的ELISA与VNA的一致性较强（κ = 0.839），而基于大肠杆菌的ELISA与VNA的一致性中等（κ = 0.731）。这些发现表明，这两种表达系统的SVA VP1蛋白可以作为基于elisa的SVA血清学诊断的替代方法，有助于群体免疫评估和疾病控制。

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Evaluating the performance of VP1 expressed in baculovirus and Escherichia coli expressed from Senecavirus A in pig using an ELISA

Senecavirus A (SVA) causes porcine idiopathic vesicular disease (PIVD), leading to economic losses in swine production. Rapid antibody detection is essential for monitoring herd immunity and controlling outbreaks. This study developed indirect ELISAs using SVA VP1 protein expressed in baculovirus and Escherichia coli (E. coli) systems. The optimized ELISAs showed high sensitivity (baculovirus: 100 %, E. coli: 96.67 %) and specificity (both 96.67 %) with a cut-off of 0.40. Both correlated strongly with virus neutralization assays (VNA) and showed no cross-reactivity with other porcine pathogens. The agreement with VNA was strong (κ = 0.839) for the baculovirus-based ELISA and moderate (κ = 0.731) for the E. coli-based ELISA. These findings suggest that SVA VP1 protein from both expression systems can serve as an alternative for ELISA-based serological diagnosis of SVA, aiding in herd immunity assessment and disease control.

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来源期刊

Veterinary journal 农林科学-兽医学

CiteScore

4.10

自引率

4.50%

发文量

审稿时长

40 days

期刊介绍： The Veterinary Journal (established 1875) publishes worldwide contributions on all aspects of veterinary science and its related subjects. It provides regular book reviews and a short communications section. The journal regularly commissions topical reviews and commentaries on features of major importance. Research areas include infectious diseases, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology and oncology.