Virus research最新文献_第6页

Efficacy assessment of antiretroviral drugs against equine infectious anemia virus in vitro 抗逆转录病毒药物体外抗马传染性贫血病毒的疗效评价。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199503

Cécile Schimmich , Astrid Vabret , José-Carlos Valle-Casuso

{"title":"Efficacy assessment of antiretroviral drugs against equine infectious anemia virus in vitro","authors":"Cécile Schimmich , Astrid Vabret , José-Carlos Valle-Casuso","doi":"10.1016/j.virusres.2024.199503","DOIUrl":"10.1016/j.virusres.2024.199503","url":null,"abstract":"<div><div>Equine infectious anemia virus (EIAV) is an equine <em>lentivirus</em> related to human immunodeficiency virus type 1 (HIV-1). Both viruses are related among the <em>Retroviridae</em> family, but their clinical manifestations are different as EIAV causes a long persistent infection with no progressive immune dysfunction in most cases. Today, no treatment is approved against EIAV, contrary to HIV-1, manageable through antiretroviral therapy, known as HAART (highly active antiretroviral therapy) or cART (combination antiretroviral therapy). No information about the efficacy of antiretroviral drugs against EIAV is available in the literature. This study evaluates the <em>in vitro</em> antiviral effect of eighteen FDA-approved antiretroviral compounds from different drug families, in an equine cells <em>in vitro</em> infection model with EIAV reference strain. Equine dermal cells, as well as equine peripheral blood mononuclear cells were treated with non-cytotoxic drug concentrations and infected with EIAV. Relative virus release in culture supernatants was assessed through relative quantification of viral RNA via RTqPCR and viral DNA comprising proviral integration in the cell genome was assessed through qPCR of infected cells, both after nucleic acid extractions. Out of eighteen tested drugs, thirteen showed a significant antiviral effect against EIAV <em>in vitro</em>, an interesting discovery showing the similarities between HIV-1 and EIAV and opening a possibility to treat equine infectious anemia to avoid the disease spread.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199503"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142755710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum 松树根腐病病原体 Heterobasidion annosum 的病毒传播频率。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199467

Elina Roininen , Eeva Johanna Vainio , Suvi Sutela , Anna Poimala , Muhammad Kashif , Tuula Piri , Jarkko Hantula

{"title":"Virus transmission frequencies in the pine root rot pathogen Heterobasidion annosum","authors":"Elina Roininen , Eeva Johanna Vainio , Suvi Sutela , Anna Poimala , Muhammad Kashif , Tuula Piri , Jarkko Hantula","doi":"10.1016/j.virusres.2024.199467","DOIUrl":"10.1016/j.virusres.2024.199467","url":null,"abstract":"<div><div>The combined use of Heterobasidion partitiviruses 13 and 15 (HetPV13-an1 and HetPV15-pa1) is considered a promising biocontrol approach against Heterobasidion root and butt rot. In a previous study, the transmission frequency of HetPV15-pa1 was found to be higher from a double partitivirus-infected donor than from a single partitivirus-infected donor. In this study, we included a wider array of recipient isolates to assess whether the phenomenon is widespread across different host strains and conducted transmission experiments on artificial media (<em>in vitro</em>) using a total of 45 different <em>H. annosum</em> donor-recipient pairs. In addition to investigating whether double partitivirus infection improves the transmission of HetPV13-an1 and HetPV15-pa1, we examined for the first time how efficiently co-infecting ssRNA viruses are concomitantly transmitted with the partitiviruses, and whether pre-existing ssRNA viruses in the recipients affect virus transmission. Generally, the transmission rates of HetPV13-an1 and HetPV15-pa1 were high from both single partitivirus-infected and double partitivirus-infected donors to most of the <em>H. annosum</em> recipient strains, with few exceptions. However, in contrast to previous experiments, the transmission frequency was not higher from the double partitivirus-infected donors. Also, ourmiavirus was transmitted between <em>H. annosum</em> strains, but the presence of another ourmiavirus in the recipient might affect the efficacy.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199467"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wild Brazilian yellow fever virus infection in Syrian hamsters (Mesocricetus auratus): Clinical and histopathological analyses 野生巴西黄热病病毒在叙利亚仓鼠中的感染：临床和组织病理学分析。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199505

Fernanda de Oliveira Bottino , Barbara Cristina Euzebio Pereira Dias de Oliveira , João Paulo Rodrigues dos Santos , Mariana Barata Viana Tiradentes , Yuli Rodrigues Maia de Souza , Tainah Silva Galdino de Paula , Hyago da Silva Medeiros Elido , Isabele Barbieri dos Santos , Ieda Pereira Ribeiro , Myrna Cristina Bonaldo , Marcelo Pelajo Machado , Pedro Paulo de Abreu Manso

{"title":"Wild Brazilian yellow fever virus infection in Syrian hamsters (Mesocricetus auratus): Clinical and histopathological analyses","authors":"Fernanda de Oliveira Bottino , Barbara Cristina Euzebio Pereira Dias de Oliveira , João Paulo Rodrigues dos Santos , Mariana Barata Viana Tiradentes , Yuli Rodrigues Maia de Souza , Tainah Silva Galdino de Paula , Hyago da Silva Medeiros Elido , Isabele Barbieri dos Santos , Ieda Pereira Ribeiro , Myrna Cristina Bonaldo , Marcelo Pelajo Machado , Pedro Paulo de Abreu Manso","doi":"10.1016/j.virusres.2024.199505","DOIUrl":"10.1016/j.virusres.2024.199505","url":null,"abstract":"<div><div>The Yellow Fever virus (YFV) wild-type strains studied until now have little or no ability to evade the Syrian hamster interferon antiviral response. Thus, evaluating the susceptibility of this model to new YFV isolates is paramount to aid in the understanding of their viscerotropic phenotype. To this end, Syrian hamsters were inoculated intraperitoneally with two Brazilian wild-type YFV isolates originated from dying or dead howler monkeys obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408) and Rio de Janeiro in 2019 (RJ155). The results were compared with a YFV experimental vaccine strain (17DD<sub>exp</sub>). The main findings observed for animals infected with the PR4408 strain were progressive weight loss and persistent viremia (at least up to day seven post-infection), associated with viral RNA detection in the liver, and hepatic, splenic, and pancreatic histological alterations consistent with YF. The infection was eliminated seven days post-infection in animals inoculated with the RJ155 strain. No changes were observed for animals infected with 17DD<sub>exp</sub> virus. The findings indicate that both Brazilian isolates are able to infect Syrian hamsters, resulting in histopathological changes compatible with the YF pathology observed in humans. Furthermore, the PR4408 strain exhibited increased virulence in this mammalian model, despite causing a non-fatal infection.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199505"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667703/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virus Research: 40 years and still going strong 病毒研究：40 年如一日

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199493

Ben Berkhout , Esteban Domingo , Nobuhiro Suzuki

引用次数: 0

Engineering a robust infectious clone and gene silencing vector from blackberry yellow vein associated virus 从黑莓黄脉相关病毒中提取强感染性克隆和基因沉默载体。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199488

Andrea Sierra-Mejia , Dan E.V. Villamor , Aaron Rocha , William M. Wintermantel , Ioannis E. Tzanetakis

{"title":"Engineering a robust infectious clone and gene silencing vector from blackberry yellow vein associated virus","authors":"Andrea Sierra-Mejia , Dan E.V. Villamor , Aaron Rocha , William M. Wintermantel , Ioannis E. Tzanetakis","doi":"10.1016/j.virusres.2024.199488","DOIUrl":"10.1016/j.virusres.2024.199488","url":null,"abstract":"<div><div>Criniviruses are emerging pathogens responsible for significant disease outbreaks worldwide. Among them, blackberry yellow vein-associated virus (BYVaV) is prevalent in blackberry-producing areas of the United States and, when present in the blackberry yellow vein disease complex with other viruses, can lead to substantial crop losses. To better understand BYVaV biology and its role in virus complex disease development, we developed a BYVaV-derived infectious clone and a virus-induced gene silencing (VIGS) vector. The infectious clone successfully induced systemic infection and symptom development in <em>Nicotiana benthamiana</em>. Additionally, transmission of the recombinant virus to indicator plants was confirmed using the whitefly vector <em>Trialeurodes vaporariorum</em>. The infectious clone was subsequently modified into a VIGS vector, with the foreign insert remaining stable for the length of the study. This work provides essential tools for advancing the study of BYVaV biology and conducting genomic studies in its natural hosts.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199488"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142569647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito 参与鲣鱼干发酵的曲霉真菌中的霉菌病毒。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199470

Seiji Buma , Syun-ichi Urayama , Rei Suo , Shiro Itoi , Shigeru Okada , Akihiro Ninomiya

{"title":"Mycoviruses from Aspergillus fungi involved in fermentation of dried bonito","authors":"Seiji Buma , Syun-ichi Urayama , Rei Suo , Shiro Itoi , Shigeru Okada , Akihiro Ninomiya","doi":"10.1016/j.virusres.2024.199470","DOIUrl":"10.1016/j.virusres.2024.199470","url":null,"abstract":"<div><div>Fungi are exploited for fermentation of foods such as cheese, Japanese sake, and soy sauce. However, the diversity of viruses that infect fungi involved in food fermentation is poorly understood. Fermented dried bonito (“katsuobushi”) is one of the most important processed marine products in Japan. Fungi involved in katsuobushi fermentation are called katsuobushi molds, and <em>Aspergillus</em> spp. have been reported to be dominant on the surface of katsuobushi during fermentation. Because various mycoviruses have been found in members of the genus <em>Aspergillus</em>, we hypothesized that katsuobushi molds are also infected with mycoviruses. Here, we describe seven novel mycoviruses belonging to six families (<em>Chrysoviridae, Fusariviridae, Mitoviridae, Partitiviridae, Polymycoviridae</em>, and <em>Pseudototiviridae</em>) from isolated katsuobushi molds (<em>Aspergillus chevalieri</em> and <em>A. sulphureus</em>) detected by fragmented and primer-ligated double-stranded RNA sequencing. Aspergillus chevalieri fusarivirus 1 has a unique bi-segmented genome, whereas other known fusariviruses have a single genomic segment. Phenotypic comparison between the parental <em>A. chevalieri</em> strain infected with Aspergillus chevalieri polymycovirus 1 (AchPmV1) and isogenic AchPmV1-free isolates indicated that AchPmV1 inhibits the early growth of the host. This study reveals the diversity of mycoviruses that infect katsuobushi molds, and provides insight into the effect of mycoviruses on fungi involved in fermentation.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199470"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complex transmission of partiti-, ambi- and ourmiaviruses in the forest pathogen Heterobasidion parviporum 森林病原体 Heterobasidion parviporum 中 partiti、ambi 和 ourmiaviruses 的复杂传播。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199466

Muhammad Kashif , Anna Poimala , Eeva J. Vainio , Suvi Sutela , Tuula Piri , László Benedek Dálya , Jarkko Hantula

{"title":"Complex transmission of partiti-, ambi- and ourmiaviruses in the forest pathogen Heterobasidion parviporum","authors":"Muhammad Kashif , Anna Poimala , Eeva J. Vainio , Suvi Sutela , Tuula Piri , László Benedek Dálya , Jarkko Hantula","doi":"10.1016/j.virusres.2024.199466","DOIUrl":"10.1016/j.virusres.2024.199466","url":null,"abstract":"<div><div>Utilizing Heterobasidion partitivirus 13 strain an1 (HetPV13-an1) and 15 strain pa1 (HetPV15-pa1) in co-infection is considered a potential biocontrol approach against Heterobasidion root and butt rot. Both partitiviruses mediate debilitating effects in most <em>Heterobasidion</em> host isolates and are generally transmitted efficiently between host strains. In this investigation, we conducted transmission experiments in the laboratory (<em>in vitro</em>) using several <em>H. parviporum</em> isolates to test whether using dual partitivirus infections is a more efficient way of transmitting viruses to new hosts compared to using single partitivirus infections, and whether co-occurring single-stranded RNA (ssRNA) viruses are co-transmitted during the process. The results showed that <em>H. parviporum</em> donors carrying both partitiviruses, HetPV13-an1 and HetPV15-pa1, transmitted HetPV15-pa1 more efficiently to recipients than the same donors infected with only HetPV15-pa1. In contrast, the transmission of HetPV13-an1 did not differ significantly between donors infected with both or only one partitivirus. Altogether, the transmission rates of HetPV13-an1 and HetPV15-pa1 were high on artificial media. Moreover, the transmission of the ssRNA viruses Heterobasidion ourmia-like virus 1(HetOlV1-pa7) and 4 (HetOlV4-an1) as well as Heterobasidion ambi-like virus 3 (HetAlV3-pa4) across different recipients were found to be variable. This study demonstrated for the first time the transmission of ambi- and ourmiaviruses between <em>H. parviporum</em> isolates in dual cultures and showed that <em>H. parviporum</em> mycelia can be cured of these ssRNA viruses using heat treatment.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199466"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736393/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5 原代巨噬细胞暴露于HESN， LTNP和慢性HIV-1感染者血清中CCR5表达和R5 HIV-1感染的调节，这些患者有或没有CCR5的天然抗体

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199506

Iole Farina , Mauro Andreotti , Claudia Pastori , Roberta Bona , Clementina Maria Galluzzo , Roberta Amici , Cristina Purificato , Caterina Uberti-Foppa , Agostino Riva , Maria Cristina Gauzzi , Lucia Lopalco , Laura Fantuzzi

{"title":"Modulation of CCR5 expression and R5 HIV-1 infection in primary macrophages exposed to sera from HESN, LTNP, and chronically HIV-1 infected people with or without natural antibodies to CCR5","authors":"Iole Farina , Mauro Andreotti , Claudia Pastori , Roberta Bona , Clementina Maria Galluzzo , Roberta Amici , Cristina Purificato , Caterina Uberti-Foppa , Agostino Riva , Maria Cristina Gauzzi , Lucia Lopalco , Laura Fantuzzi","doi":"10.1016/j.virusres.2024.199506","DOIUrl":"10.1016/j.virusres.2024.199506","url":null,"abstract":"<div><div>CCR5 is the main co-receptor for HIV-1 cell entry and it plays key roles in HIV-1 mucosal transmission. Natural anti-CCR5 antibodies were found in HIV-1-exposed seronegative and long-term non-progressor subjects, suggesting a role in controlling viral replication <em>in vivo</em>. We assessed the effect of sera containing or not natural anti-CCR5 antibodies, on membrane CCR5 level and HIV-1 infection in primary macrophages. Sera modulated CCR5 expression with a trend dependent on the donor/serum tested but independent on the presence or absence of anti-CCR5 antibodies. All sera strongly reduced HIV-1 DNA in all donor's macrophages and no correlation was observed between CCR5 and viral DNA levels. These results suggest that CCR5 expression level is not a major determinant of macrophage infection and that the observed modulation of CCR5 and HIV-1 DNA might depend on factors other than CCR5-reactive antibodies present in sera and/or intrinsic to the donors on which sera were tested.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199506"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669954/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142787171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of an infectious clone and gene silencing vector derived from blackberry chlorotic ringspot virus 从黑莓萎黄环斑病毒中提取的传染性克隆和基因沉默载体的开发与应用。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199460

Andrea Sierra-Mejia, Dan E.V. Villamor, Ioannis E. Tzanetakis

引用次数: 0

CircPTPN11 inhibits the replication of Coxsackievirus B5 through regulating the IFN-I pathway by targeting miR-152-3p/SIRPA axis CircPTPN11通过靶向miR-152-3p/SIRPA轴调控IFN-I通路抑制柯萨奇病毒B5的复制。

IF 2.5 4区医学

Virus research Pub Date : 2024-12-01 DOI: 10.1016/j.virusres.2024.199508

Jingru Gao , Fan Yang , Jihong Zhang , Heng Yang , Wei Chen

{"title":"CircPTPN11 inhibits the replication of Coxsackievirus B5 through regulating the IFN-I pathway by targeting miR-152-3p/SIRPA axis","authors":"Jingru Gao , Fan Yang , Jihong Zhang , Heng Yang , Wei Chen","doi":"10.1016/j.virusres.2024.199508","DOIUrl":"10.1016/j.virusres.2024.199508","url":null,"abstract":"<div><div>Coxsackievirus B5 (CVB5) is a major pathogen responsible for hand-foot-mouth disease, herpangina, and even severe death. The mechanisms underlying CVB5-induced diseases are not fully elucidated, and no specific antiviral treatments are currently available. Circular RNAs (circRNAs), a closed-loop molecular structure, have been reported to be involved in virus infectious diseases. However, their roles and mechanisms in CVB5 infection remain largely unknown. In this study, we identify that CircPTPN11 is significantly upregulated following CVB5 infection in RD cells. Characteristic analysis reveals that the expression of CircPTPN11 is both time- and dose-dependent upon CVB5 infection and is specific to intestinal tissue. Moreover, CircPTPN11 inhibits CVB5 replication by activating IRF3 in the type-I interferon (IFN-I) pathway. Further underneath mechanism shows that CircPTPN11 indirectly regulates CVB5 replication by sponging miR-152-3p, and miR-152-3p influences CVB5 replication by interacting with the gene coding for signal regulatory protein alpha (SIRPA). In conclusion, this study suggests that CircPTPN11 targets SIRPA by sponging miR-152-3p, thereby inhibiting the replication and proliferation of CVB5. These findings provide a molecular target for the diagnosis and treatment of CVB5 infection.</div></div>","PeriodicalId":23483,"journal":{"name":"Virus research","volume":"350 ","pages":"Article 199508"},"PeriodicalIF":2.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0