Tissue Barriers最新文献_第6页

The correlation between fecal microbiota profiles and intracellular junction genes expression in young Iranian patients with celiac disease. 伊朗年轻乳糜泻患者粪便微生物群谱与细胞内连接基因表达之间的相关性。

IF 3.1

Tissue Barriers Pub Date : 2024-05-02 DOI: 10.1080/21688370.2024.2347766

Mohadeseh Mahmoudi Ghehsareh, Nastaran Asri, Fahimeh Sadat Gholam-Mostafaei, Hamidreza Houri, Flora Forouzesh, Shokoufeh Ahmadipour, Somayeh Jahani-Sherafat, Mohammad Rostami-Nejad, Pasquale Mansueto, Aurelio Seidita

{"title":"The correlation between fecal microbiota profiles and intracellular junction genes expression in young Iranian patients with celiac disease.","authors":"Mohadeseh Mahmoudi Ghehsareh, Nastaran Asri, Fahimeh Sadat Gholam-Mostafaei, Hamidreza Houri, Flora Forouzesh, Shokoufeh Ahmadipour, Somayeh Jahani-Sherafat, Mohammad Rostami-Nejad, Pasquale Mansueto, Aurelio Seidita","doi":"10.1080/21688370.2024.2347766","DOIUrl":"https://doi.org/10.1080/21688370.2024.2347766","url":null,"abstract":"Celiac disease (CD) is characterized by the disruption of the intestinal barrier integrity and alterations in the microbiota composition. This study aimed to evaluate the changes in the fecal microbiota profile and mRNA expressions of intracellular junction-related genes in pediatric patients with CD compared to healthy controls (HCs). Thirty treated CD patients, 10 active CD, and 40 HCs were recruited. Peripheral blood (PB) and fecal samples were collected. Microbiota analysis was performed using quantitative real-time PCR (qPCR) test. The mRNA expressions of ZO-1, occludin, β-catenin, E-cadherin, and COX-2 were also evaluated. In active and treated CD patients, the PB expression levels of ZO-1 (p = 0.04 and 0.002, respectively) and β-catenin (p = 0.006 and 0.02, respectively) were lower than in HCs. PB Occludin's level was upregulated in both active and treated CD patients compared to HCs (p = 0.04 and 0.02, respectively). However, PB E-cadherin and COX-2 expression levels and fecal mRNA expressions of ZO-1, occludin, and COX-2 did not differ significantly between cases and HCs (P˃0.05). Active CD patients had a higher relative abundance of the Firmicutes (p = 0.04) and Actinobacteria (p = 0.03) phyla compared to treated subjects. The relative abundance of Veillonella (p = 0.04) and Staphylococcus (p = 0.01) genera was lower in active patients in comparison to HCs. Researchers should explore the precise impact of the gut microbiome on the molecules and mechanisms involved in intestinal damage of CD. Special attention should be given to Bifidobacteria and Enterobacteriaceae, as they have shown a significant correlation with the expression of tight junction-related genes.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2347766"},"PeriodicalIF":3.1,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140870223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spermine oxidase inhibitor, MDL 72527, reduced neovascularization, vascular permeability, and acrolein-conjugated proteins in a mouse model of ischemic retinopathy. 精胺氧化酶抑制剂 MDL 72527 可减少缺血性视网膜病变小鼠模型中的新生血管形成、血管通透性和丙烯醛结合蛋白。

IF 3.1

Tissue Barriers Pub Date : 2024-04-29 DOI: 10.1080/21688370.2024.2347070

Abdullah Alhumaid, Fang Liu, Shengshuai Shan, Eissa Jafari, Nadia Nourin, Payaningal R Somanath, S Priya Narayanan

{"title":"Spermine oxidase inhibitor, MDL 72527, reduced neovascularization, vascular permeability, and acrolein-conjugated proteins in a mouse model of ischemic retinopathy.","authors":"Abdullah Alhumaid, Fang Liu, Shengshuai Shan, Eissa Jafari, Nadia Nourin, Payaningal R Somanath, S Priya Narayanan","doi":"10.1080/21688370.2024.2347070","DOIUrl":"10.1080/21688370.2024.2347070","url":null,"abstract":"Disruptions in polyamine metabolism have been identified as contributing factors to various central nervous system disorders. Our laboratory has previously highlighted the crucial role of polyamine oxidation in retinal disease models, specifically noting elevated levels of spermine oxidase (SMOX) in inner retinal neurons. Our prior research demonstrated that inhibiting SMOX with MDL 72527 protected against vascular injury and microglial activation induced by hyperoxia in the retina. However, the effects of SMOX inhibition on retinal neovascularization and vascular permeability, along with the underlying molecular mechanisms of vascular protection, remain incompletely understood. In this study, we utilized the oxygen-induced retinopathy (OIR) model to explore the impact of SMOX inhibition on retinal neovascularization, vascular permeability, and the molecular mechanisms underlying MDL 72527-mediated vasoprotection in the OIR retina. Our findings indicate that inhibiting SMOX with MDL 72527 mitigated vaso-obliteration and neovascularization in the OIR retina. Additionally, it reduced OIR-induced vascular permeability and Claudin-5 expression, suppressed acrolein-conjugated protein levels, and downregulated P38/ERK1/2/STAT3 signaling. Furthermore, our results revealed that treatment with BSA-Acrolein conjugates significantly decreased the viability of human retinal endothelial cells (HRECs) and activated P38 signaling. These observations contribute valuable insights into the potential therapeutic benefits of SMOX inhibition by MDL 72527 in ischemic retinopathy.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2347070"},"PeriodicalIF":3.1,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human in vitro blood barrier models: architectures and applications. 人体体外血液屏障模型：结构与应用。

IF 3.6

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-06-20 DOI: 10.1080/21688370.2023.2222628

Brittany E Watson, Julia A Miles, Melissa A Moss

引用次数: 0

Models for barrier understanding in health and disease in lab-on-a-chips. 芯片实验室中了解健康和疾病障碍的模型。

IF 3.1

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-06-09 DOI: 10.1080/21688370.2023.2221632

J Ponmozhi, S Dhinakaran, Dorottya Kocsis, Kristóf Iván, Franciska Erdő

{"title":"Models for barrier understanding in health and disease in lab-on-a-chips.","authors":"J Ponmozhi, S Dhinakaran, Dorottya Kocsis, Kristóf Iván, Franciska Erdő","doi":"10.1080/21688370.2023.2221632","DOIUrl":"10.1080/21688370.2023.2221632","url":null,"abstract":"The maintenance of body homeostasis relies heavily on physiological barriers. Dysfunction of these barriers can lead to various pathological processes, including increased exposure to toxic materials and microorganisms. Various methods exist to investigate barrier function in vivo and in vitro. To investigate barrier function in a highly reproducible manner, ethically, and high throughput, researchers have turned to non-animal techniques and micro-scale technologies. In this comprehensive review, the authors summarize the current applications of organ-on-a-chip microfluidic devices in the study of physiological barriers. The review covers the blood-brain barrier, ocular barriers, dermal barrier, respiratory barriers, intestinal, hepatobiliary, and renal/bladder barriers under both healthy and pathological conditions. The article then briefly presents placental/vaginal, and tumour/multi-organ barriers in organ-on-a-chip devices. Finally, the review discusses Computational Fluid Dynamics in microfluidic systems that integrate biological barriers. This article provides a concise yet informative overview of the current state-of-the-art in barrier studies using microfluidic devices.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2221632"},"PeriodicalIF":3.1,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11042069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9967899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unfolded protein response suppression potentiates LPS-induced barrier dysfunction and inflammation in bovine pulmonary artery endothelial cells. 抑制折叠蛋白反应可增强 LPS 诱导的牛肺动脉内皮细胞屏障功能障碍和炎症。

IF 3.1

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-07-12 DOI: 10.1080/21688370.2023.2232245

Nektarios Barabutis, Mohammad S Akhter

引用次数: 0

Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers. 培养基和培养方式会改变猪结肠单层的细胞组成和屏障完整性。

IF 3.1

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-06-21 DOI: 10.1080/21688370.2023.2222632

Alicia M Barnett, Jane A Mullaney, Warren C McNabb, Nicole C Roy

{"title":"Culture media and format alter cellular composition and barrier integrity of porcine colonoid-derived monolayers.","authors":"Alicia M Barnett, Jane A Mullaney, Warren C McNabb, Nicole C Roy","doi":"10.1080/21688370.2023.2222632","DOIUrl":"10.1080/21688370.2023.2222632","url":null,"abstract":"Intestinal organoid technology has revolutionized our approach to in vitro cell culture due in part to their three-dimensional structures being more like the native tissue from which they were derived with respect to cellular composition and architecture. For this reason, organoids are becoming the new gold standard for undertaking intestinal epithelial cell research. Unfortunately, their otherwise advantageous three-dimensional geometry prevents easy access to the apical epithelium, which is a major limitation when studying interactions between dietary or microbial components and host tissues. To overcome this problem, we developed porcine colonoid-derived monolayers cultured on both permeable Transwell inserts and tissue culture treated polystyrene plates. We found that seeding density and culture format altered the expression of genes encoding markers of specific cell types (stem cells, colonocytes, goblets, and enteroendocrine cells), and barrier maturation (tight junctions). Additionally, we found that changes to the formulation of the culture medium altered the cellular composition of colonoids and of monolayers derived from them, resulting in cultures with an increasingly differentiated phenotype that was similar to that of their tissue of origin.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2222632"},"PeriodicalIF":3.1,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11042055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9669957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

(Zebra)fishing for nephrogenesis genes. (斑马）寻找肾脏生成基因。

IF 3.6

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-05-31 DOI: 10.1080/21688370.2023.2219605

Brooke E Chambers, Nicole E Weaver, Caroline M Lara, Thanh Khoa Nguyen, Rebecca A Wingert

引用次数: 0

Exosomes: current knowledge and future perspectives. 外泌体：现有知识和未来展望。

IF 3.6

Tissue Barriers Pub Date : 2024-04-02 Epub Date: 2023-07-13 DOI: 10.1080/21688370.2023.2232248

Swati Singh, Deepraj Paul, Virendra Nath, Rohini A

{"title":"Exosomes: current knowledge and future perspectives.","authors":"Swati Singh, Deepraj Paul, Virendra Nath, Rohini A","doi":"10.1080/21688370.2023.2232248","DOIUrl":"10.1080/21688370.2023.2232248","url":null,"abstract":"Exosomes are membrane-bound micro-vesicles that possess endless therapeutic potential for treatment of numerous pathologies including autoimmune, cardiovascular, ocular, and nervous disorders. Despite considerable knowledge about exosome biogenesis and secretion, still, there is a lack of information regarding exosome uptake by cell types and internal signaling pathways through which these exosomes process cellular response. Exosomes are key components of cell signaling and intercellular communication. In central nervous system (CNS), exosomes can penetrate BBB and maintain homeostasis by myelin sheath regulation and the waste products elimination. Therefore, the current review summarizes role of exosomes and their use as biomarkers in cardiovascular, nervous and ocular disorders. This aspect of exosomes provides positive hope to monitor disease development and enable early diagnosis and treatment optimization. In this review, we have summarized recent findings on physiological and therapeutic effects of exosomes and also attempt to provide insights about stress-preconditioned exosomes and stem cell-derived exosomes.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2232248"},"PeriodicalIF":3.6,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11042064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9776715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fibroblasts/three-dimensional scaffolds complexes promote wound healing in rats with skin defects. 成纤维细胞/三维支架复合物可促进皮肤缺损大鼠的伤口愈合。

IF 3.1

Tissue Barriers Pub Date : 2024-03-27 DOI: 10.1080/21688370.2024.2334544

Ting Jiang, Qiang Liu, Er-Chang Xu, Si-Yu He, Hong-Yan Liu, Chao Tian, Lan-Fang Zhang, Ze-Long Yang

{"title":"Fibroblasts/three-dimensional scaffolds complexes promote wound healing in rats with skin defects.","authors":"Ting Jiang, Qiang Liu, Er-Chang Xu, Si-Yu He, Hong-Yan Liu, Chao Tian, Lan-Fang Zhang, Ze-Long Yang","doi":"10.1080/21688370.2024.2334544","DOIUrl":"https://doi.org/10.1080/21688370.2024.2334544","url":null,"abstract":"We aim to construct a three-dimensional nano-skin scaffold material in vitro and study its promoting effect on wound healing in vivo. In this study, hybrid constructs of three-dimensional (3D) scaffolds were successfully fabricated by combination of type I collagen (COL-1) and polylactic-glycolic acid (PLGA). Fibroblasts and human umbilical cord mesenchymal stem cells (hUCMSCs) were used to implanted into 3D scaffolds and constructed into SD skin scaffolds in vitro. Finally, the fibroblasts/scaffolds complexes were inoculated on the surface of rat wound skin to study the promoting effect of the complex on wound healing. In our study, we successfully built a 3D scaffold, which had a certain porosity. Meanwhile, the content of COL-1 in the cell supernatant of fibroblast/scaffold complexes was increased. Furthermore, the expression of F-actin, CD105, integrin β, VEGF, and COL-1 was up-regulated in hUCMSC/scaffold complexes compared with the control group. In vivo, fibroblast/scaffold complexes promoted wound healing in rats. Our data suggested that the collagen Ⅳ and vimentin were elevated and collagen fibers were neatly arranged in the fibroblast/scaffold complex group was significantly higher than that in the scaffold group. Taken together, fibroblast/scaffold complexes were expected to be novel materials for treating skin defects.","PeriodicalId":23469,"journal":{"name":"Tissue Barriers","volume":" ","pages":"2334544"},"PeriodicalIF":3.1,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140307054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inter-claudin antagonism of paracellular pore function: mechanism and beyond. 细胞旁孔功能的克隆蛋白间拮抗作用：机制与超越。

IF 3.1

Tissue Barriers Pub Date : 2024-03-17 DOI: 10.1080/21688370.2024.2330773

Wanapas Wachiradejkul, Pawin Pongkorpsakol

引用次数: 0