Tuberculosis最新文献_第3页

Differential expression of vascular endothelial growth factor A (VEGFA) and M1 macrophage marker nitric oxide synthase 2 (NOS2) in lymph node granulomas of BCG-vaccinated and non-vaccinated cattle infected with Mycobacterium bovis

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-22 DOI: 10.1016/j.tube.2025.102609

C Kanipe , EJ Putz , MV Palmer

{"title":"Differential expression of vascular endothelial growth factor A (VEGFA) and M1 macrophage marker nitric oxide synthase 2 (NOS2) in lymph node granulomas of BCG-vaccinated and non-vaccinated cattle infected with Mycobacterium bovis","authors":"C Kanipe , EJ Putz , MV Palmer","doi":"10.1016/j.tube.2025.102609","DOIUrl":"10.1016/j.tube.2025.102609","url":null,"abstract":"<div><div>Bovine tuberculosis is mainly caused by <em>Mycobacterium bovis</em>. Bacillus Calmette-Guérin (BCG) is an attenuated strain of <em>M. bovis</em> which provides variable disease protection. Lesions have been characterized in infected cattle, but little comparison has been done with lesions which form in BCG-vaccinates. Here, <em>in situ</em> hybridization examined differences in expression of <em>M. bovis</em> RNA, inducible nitric oxide synthase 2, and vascular endothelial growth factor A in relation to vaccination status and granuloma grade, using two different groups of cattle. Data found no differences between vaccination groups or granuloma grade in average copies of <em>M. bovis</em> mRNA per μm<sup>2</sup> of total granuloma area or per μm<sup>2</sup> of necrotic areas. Within a vaccination group high-grade granulomas had more NOS2 per cell, per μm<sup>2</sup> and a higher percentage of cells expressing NOS2 than low-grade granulomas. Non-vaccinates had a higher percentage of cells producing NOS2 than vaccinates. Differences in NOS2 expression varied by group. Vaccination status and granuloma grade did not affect the average copies of VEGFA per cell or the percent of cells expressing RNA, however VEGFA copies per μm<sup>2</sup> varied between groups. These findings suggest NOS2 and VEGFA are likely not mechanisms of BCG vaccination protection but may impact disease severity.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"151 ","pages":"Article 102609"},"PeriodicalIF":2.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluctuations in circulating cell-free mitochondrial and nuclear DNA copy numbers in blood plasma after anti-tuberculosis drug intake in patients with drug-susceptible tuberculosis

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-21 DOI: 10.1016/j.tube.2025.102611

Lauma Freimane , Agnija Kivrāne , Viktorija Ulanova , Anda Vīksna , Eduards Sevostjanovs , Solveiga Grīnberga , Andra Cīrule , Alvils Krams , Renāte Ranka

{"title":"Fluctuations in circulating cell-free mitochondrial and nuclear DNA copy numbers in blood plasma after anti-tuberculosis drug intake in patients with drug-susceptible tuberculosis","authors":"Lauma Freimane , Agnija Kivrāne , Viktorija Ulanova , Anda Vīksna , Eduards Sevostjanovs , Solveiga Grīnberga , Andra Cīrule , Alvils Krams , Renāte Ranka","doi":"10.1016/j.tube.2025.102611","DOIUrl":"10.1016/j.tube.2025.102611","url":null,"abstract":"<div><div>Biomarker research characterising the effect of anti-tuberculosis (TB) chemotherapy on systemic body response is still limited. In this study, we aimed to investigate fluctuations in circulating cell-free mitochondrial DNA (ccf-mtDNA) and circulating cell-free nuclear DNA (ccf-nDNA) copy number (CN) in blood plasma of patients with drug-susceptible TB (DS-TB) and to decipher factors related to these fluctuations.</div><div>The results showed considerable changes in ccf-mtDNA CN in plasma samples before drug intake and 2 and 6 h afterwards, with high inter patient variability at each time point. Multivariate linear regression revealed that the dynamics of ccf-mtDNA CN was influenced by patients’ age, ethambutol pharmacokinetics, and body-mass index (BMI); ethambutol exposure emerged as the most significant factor. Very low ccf-nDNA CN in all three time points with little variation was observed; none factors were strongly associated with ccf-nDNA.</div><div>In conclusion, our study revealed the effect of anti-TB chemotherapy, age and BMI on acute changes in circulating ccf-mtDNA CN in blood plasma and highlighted the systemic, mitochondria-related effects of ethambutol in patients with TB. Further studies with larger cohorts are needed to understand the biological relevance of ccf-DNA in patients with TB and to validate its application in TB treatment monitoring.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"151 ","pages":"Article 102611"},"PeriodicalIF":2.8,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143042376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV co-infection is associated with increased HLA-DR expression by Mycobacterium tuberculosis-specific CD4 T cells in people with latent tuberculosis infection

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-20 DOI: 10.1016/j.tube.2025.102607

Jeremiah Khayumbi , Loren E. Sasser , Taryn A. McLaughlin , Joshua Ongalo , Joan Tonui , Samuel Gurrion Ouma , Angie Campbell , Felix Hayara Odhiambo , Neel R. Gandhi , Chelimo Kiprotich , Cheryl L. Day

{"title":"HIV co-infection is associated with increased HLA-DR expression by Mycobacterium tuberculosis-specific CD4 T cells in people with latent tuberculosis infection","authors":"Jeremiah Khayumbi , Loren E. Sasser , Taryn A. McLaughlin , Joshua Ongalo , Joan Tonui , Samuel Gurrion Ouma , Angie Campbell , Felix Hayara Odhiambo , Neel R. Gandhi , Chelimo Kiprotich , Cheryl L. Day","doi":"10.1016/j.tube.2025.102607","DOIUrl":"10.1016/j.tube.2025.102607","url":null,"abstract":"<div><div>Infection with HIV is associated with dysregulated CD4 T cell responses to <em>Mycobacterium tuberculosis</em> (Mtb) and increased risk of developing tuberculosis. Mtb-specific CD4 T cells in people with HIV have diminished Th1 cytokine production capacity, thus we utilized a flow cytometry-based assay to measure CD40L expression by Mtb-specific CD4 T cells in a cytokine-independent manner. We evaluated the frequency and phenotype of Mtb-specific CD4 responses in Kenyan adults with latent Mtb infection and found that the majority of Mtb-specific CD4 T cells expressed CD40L in the absence of IFN-γ, regardless of HIV infection status. Expression of HLA-DR was increased on Mtb-specific CD4 T cells in people with HIV, compared to people without HIV. These data suggest expression of HLA-DR by Mtb-specific CD4 T cells may represent an early biomarker of increased mycobacterial antigen stimulation in people with HIV prior to the development of symptomatic tuberculosis disease.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"151 ","pages":"Article 102607"},"PeriodicalIF":2.8,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microneedle-mediated intradermal delivery of Bacille Calmette-Guérin (BCG) vaccines for single-dose tuberculosis vaccination 单剂结核病疫苗接种中卡介苗的微针介导皮内递送。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-16 DOI: 10.1016/j.tube.2025.102608

Sanha Lee , Taeyoon Kim , Keum-Yong Seong , Sang-Gu Yim , Won-Kyu Lee , Semin Kim , Kang-Oh Lee , Seung Yun Yang , Sungweon Ryoo

{"title":"Microneedle-mediated intradermal delivery of Bacille Calmette-Guérin (BCG) vaccines for single-dose tuberculosis vaccination","authors":"Sanha Lee , Taeyoon Kim , Keum-Yong Seong , Sang-Gu Yim , Won-Kyu Lee , Semin Kim , Kang-Oh Lee , Seung Yun Yang , Sungweon Ryoo","doi":"10.1016/j.tube.2025.102608","DOIUrl":"10.1016/j.tube.2025.102608","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a highly lethal infectious disease. The primary preventive measure is Bacille Calmette-Guérin (BCG), a live attenuated vaccine. However, the current intradermal vaccination method with 10-dose vials faces challenges such as inadequate infant injection, inaccurate dispensing, and unstable storage. Researchers have explored microneedle (MN) technology to address these concerns as a intradermal vaccine delivery approach. MN array patches offer painless administration, convenience, improved immunogenicity, and vaccine stability. This study aimed to develop a coated MN system using a micro-dispensing technique at a low temperature (4 °C) and specific excipients for precise dosing and vaccine viability enhancement. Long-term storage stability revealed enhanced storage stability of the BCG-coated MN (BCG-MN) vaccine, maintaining a survival rate of over 60 % for 8 weeks at −20 °C. <em>In vivo</em> vaccination tests using BCG-MN vaccines on guinea pigs exhibited no adverse reactions. Moreover, the BCG-MN vaccine demonstrated superior immune response compared to injections, suggesting that this BCG vaccine-coated MN platform has the potential as a single-dose TB vaccination technology, offering precise dosing control and enhanced immune effectiveness with high storage stability.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"151 ","pages":"Article 102608"},"PeriodicalIF":2.8,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical model of Mycobacteroides abscessus lung disease by nose-only exposure of mice to bacterial powder aerosol 通过仅鼻暴露于细菌粉末气溶胶的小鼠建立脓肿分枝杆菌肺病临床前模型。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-10 DOI: 10.1016/j.tube.2025.102606

Khushboo Verma , Tanu Garg , Shriya Singh , Venkata Siva Reddy Deivreddy , Sunil K. Raman , Reena Bharti , Hasham Shafi Sofi , Kavita Singh , Mehazabeen Shaik , Arunava Dasgupta , Madhav N. Mugale , Amit Misra

{"title":"Preclinical model of Mycobacteroides abscessus lung disease by nose-only exposure of mice to bacterial powder aerosol","authors":"Khushboo Verma , Tanu Garg , Shriya Singh , Venkata Siva Reddy Deivreddy , Sunil K. Raman , Reena Bharti , Hasham Shafi Sofi , Kavita Singh , Mehazabeen Shaik , Arunava Dasgupta , Madhav N. Mugale , Amit Misra","doi":"10.1016/j.tube.2025.102606","DOIUrl":"10.1016/j.tube.2025.102606","url":null,"abstract":"<div><div>The limitations of existing mouse models of lung infection with <em>Mycobacteroides abscessus</em> impede drug discovery and development. In contrast to current animal models that introduce NTM intravenously or by intranasal/intra-tracheal instillation or via bronchoscopy-guided insufflation, we developed a dry powder inhalation (DPI) of <em>M. abscessus</em> ATCC 19977 that generated paucibacillary lung infection and histopathology in immunocompetent mice. Swiss outbred mice receiving ∼1000 (3-log) colony forming units (CFU) of <em>M. abscessus</em>/gram lung tissue via the DPI administered by nose-only inhalation for 90 s showed peak bacterial burden of ∼3.35-log CFU/g in the lungs after 28 days. This was maintained at ∼2-log/g from Day 35 through 56 in the lungs, but not in the spleen. Histopathology indicated increasing severity of inflammation, fibrosis and lung consolidation. Bacteria were rarely recovered from spleen, and histopathological examination indicated partial resolution in the spleen between Days 49–56. The DPI, prepared by freeze-drying log-phase liquid culture with cryoprotectants was formulated to possess aerosol characteristics suitable for alveolar deposition. Aerosol exposure to inoculum mimics natural airborne infection. Non-invasive aerosol infection is convenient, inexpensive, does not require special equipment or extensive training and mitigates stress to animals, but biosafety level 3 containment is recommended to mitigate risk to experimenters.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"151 ","pages":"Article 102606"},"PeriodicalIF":2.8,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The immunomodulatory effects of Mesenchymal stem cells on THP-1-derived macrophages against Mycobacterium tuberculosis H37Ra infection 间充质干细胞对thp -1来源巨噬细胞抗结核分枝杆菌H37Ra感染的免疫调节作用。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-01 DOI: 10.1016/j.tube.2024.102593

Qianwei Yang , Yiqun Zhou , Waqas Farooq , Qimiao Liu , Jinhui Duan , Li Xing , Changxin Wu , Li Dong

{"title":"The immunomodulatory effects of Mesenchymal stem cells on THP-1-derived macrophages against Mycobacterium tuberculosis H37Ra infection","authors":"Qianwei Yang , Yiqun Zhou , Waqas Farooq , Qimiao Liu , Jinhui Duan , Li Xing , Changxin Wu , Li Dong","doi":"10.1016/j.tube.2024.102593","DOIUrl":"10.1016/j.tube.2024.102593","url":null,"abstract":"<div><h3>Background</h3><div>Immune imbalance is crucial in tuberculosis pathogenesis and may be modulated by mesenchymal stem cells (MSCs). However, how MSCs regulate the host's response to <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) is unclear.</div></div><div><h3>Methods</h3><div>Human umbilical cord-derived MSCs were co-cultured with <em>Mtb</em>-infected THP-1 macrophages. The intracellular release of ROS in macrophages was measured by DCFH-DA. Cytokine expression was measured by RT-qPCR, apoptosis by Annexin V/PI assay, and pyroptosis markers by Western blotting. Differentially expressed genes (DEGs) in <em>Mtb</em>-infected THP-1 co-cultured with or without MSCs were identified by RNA-seq and potential signaling pathways were analyzed through bioinformatics.</div></div><div><h3>Results</h3><div>The fibroblastic morphology of MSCs exhibited 95 % positivity for CD73, CD90, and CD105, while the positivity rate for negative marker HLA-DR was less than 2 %. In <em>Mtb</em>-infected THP-1 macrophages, co-culturing with MSCs increased ROS release, cytokines expression (<em>IL-1β</em>, <em>IL-6</em>, <em>TNF-α</em>), apoptosis, and pyroptosis markers (<em>NLRP3</em>, <em>Caspase-1</em>, and <em>GSDMD</em>). Comparative transcriptome analysis identified 347 up-regulated and 291 down-regulated DEGs, primarily associated with receptor-ligand interactions and enriched in cytokine signaling pathways including JAK-STAT, TNF, ferroptosis, and autophagy.</div></div><div><h3>Conclusion</h3><div>MSCs could enhance the macrophages’ immune response to <em>Mtb</em> by activating immune receptors and inflammatory signaling pathways.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"150 ","pages":"Article 102593"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Study on the role and molecular mechanism of METTL3-mediated miR-29a-3p in the inflammatory response of spinal tuberculosis” [Tuberculosis 148 (2024) 102546] “mettl3介导的miR-29a-3p在脊柱结核炎症反应中的作用和分子机制研究”[tb 148(2024) 102546]的更正。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-01 DOI: 10.1016/j.tube.2024.102590

Xiaojun Ma , Yuxin Gao , Zhibo Ren , Hui Dong , Xu Zhang , Ningkui Niu

引用次数: 0

Development of tetracycline analogues with increased aqueous stability for the treatment of mycobacterial infections 开发四环素类似物，提高水稳定性治疗分枝杆菌感染。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-01 DOI: 10.1016/j.tube.2024.102592

Jiuyu Liu , Gregory A. Phelps , Christine M. Dunn , Patricia A. Murphy , Laura A. Wilt , Victoria Loudon , Robin B. Lee , Dinesh Fernando , Lei Yang , Kristina N. Tran , Brennen T. Troyer , Andres Obregon-Henao , Richard E. Lee

{"title":"Development of tetracycline analogues with increased aqueous stability for the treatment of mycobacterial infections","authors":"Jiuyu Liu , Gregory A. Phelps , Christine M. Dunn , Patricia A. Murphy , Laura A. Wilt , Victoria Loudon , Robin B. Lee , Dinesh Fernando , Lei Yang , Kristina N. Tran , Brennen T. Troyer , Andres Obregon-Henao , Richard E. Lee","doi":"10.1016/j.tube.2024.102592","DOIUrl":"10.1016/j.tube.2024.102592","url":null,"abstract":"<div><div>Tetracycline analogs from the minocycline family have recently shown promise for the treatment of non-tuberculous mycobacterial infections. However, current tetracycline and minocycline therapeutics can be limited by tolerability, stability, or inactivation by TetX. In this study, a series of novel 9-heteroaryl substituted minocycline analogs were designed and synthesized, which resulted in analogs with good <em>in vitro</em> activity against <em>Mycobacterium tuberculosis</em> and <em>Mycobacterium abscessus</em>, stability in water for more than 7 days, avoidance of TetX inactivation in <em>M. abscessus,</em> and a lack of cytotoxicity in HepG2 mammalian cells. In vivo efficacy was confirmed for the tetracycline analogs in an acute model of GM-CSF KO mice infected with <em>M. abscessus</em>, displaying superior efficacy to standard-of-care antibiotic clarithromycin. Molecular modeling and potentiation assays demonstrate avoidance of MabTetX, and the structure-activity relationships of the series are discussed herein for <em>M</em>. <em>tuberculosis</em> and <em>M</em>. <em>abscessus</em>.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"150 ","pages":"Article 102592"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of pleural fluid-derived extracellular vesicles as a source of Mycobacterium tuberculosis antigens MPT51 and MPT64 for pleural TB diagnosis: a proof-of-concept study 胸腔积液源性细胞外囊泡作为结核分枝杆菌抗原 MPT51 和 MPT64 的来源在胸膜结核诊断中的应用：概念验证研究。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-01 DOI: 10.1016/j.tube.2024.102578

Neha Jindal , Pratibha Sharma , Sachin Punia , Manisha Dass , Divya Anthwal , Rakesh Kumar Gupta , Manpreet Bhalla , Ritu Singhal , Ashish Behera , Rakesh Yadav , Sunil Sethi , Sahajal Dhooria , Ashutosh Nath Aggarwal , Sagarika Haldar

{"title":"Utility of pleural fluid-derived extracellular vesicles as a source of Mycobacterium tuberculosis antigens MPT51 and MPT64 for pleural TB diagnosis: a proof-of-concept study","authors":"Neha Jindal , Pratibha Sharma , Sachin Punia , Manisha Dass , Divya Anthwal , Rakesh Kumar Gupta , Manpreet Bhalla , Ritu Singhal , Ashish Behera , Rakesh Yadav , Sunil Sethi , Sahajal Dhooria , Ashutosh Nath Aggarwal , Sagarika Haldar","doi":"10.1016/j.tube.2024.102578","DOIUrl":"10.1016/j.tube.2024.102578","url":null,"abstract":"<div><div>Extracellular vesicles (EVs) have recently emerged as a source of microbe-specific biomarkers for disease diagnosis. In the present study, we evaluated the utility of pleural fluid-derived extracellular vesicles (pEVs) as a source of <em>Mycobacterium tuberculosis</em> (<em>M</em>. <em>tb</em>.) antigens for pleural TB (pTB) diagnosis. EVs were isolated from pleural fluid (PF) samples and were characterized by scanning electron microscopy, and immunoblotting by targeting CD63 and LAMP2 markers. Antigen-detection ELISAs were developed for 2 <em>M</em><em>.</em> <em>tb</em><em>.</em>-specific antigens, MPT51 and MPT64 in pEVs (pEV-ELISA) and direct PF samples (PF-ELISA), and were evaluated on n = 86 samples in a blinded manner. Cut-off values were calculated by ROC-curve analysis to achieve 90 % (95%CI:73.47–97.89) and 86.67 % (95%CI:69.28–96.24) specificity for MPT51 and MPT64 pEV-ELISA respectively. The sensitivity of pEV-ELISA was 71.43 % (95%CI; 29.04–96.33) for MPT51 antigen and 57.14 % (95%CI; 18.41–90.1) for MPT64 antigen in the ‘Definite’ pTB group, while in the ‘Definite and Probable’ pTB group, the sensitivity was 62.86 % (95%CI:44.92–78.53) for MPT51 and 65.71 % (95%CI:47.79–80.87) for MPT64. The performance of PF-ELISA was sub-optimal, with 28.57 % (95%CI:3.67–70.96) and 14.29 % (95%CI:0.36–57.87) sensitivity for MPT51 and MPT64 in ‘Definite’ pTB group respectively. We conclude that <em>M</em>. <em>tb</em>.-antigens are concentrated in the EV-fraction of PF samples and EVs can be utilized for antigen-detection assays for pTB diagnosis.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"150 ","pages":"Article 102578"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A survey of physicians, biomedical researchers and college-educated adults in urban north India about inhaled therapies 对印度北部城市的医生、生物医学研究人员和受过大学教育的成年人进行了一项关于吸入疗法的调查。

IF 2.8 3区医学

Tuberculosis Pub Date : 2025-01-01 DOI: 10.1016/j.tube.2024.102591

Khushboo Verma , Amit Misra

{"title":"A survey of physicians, biomedical researchers and college-educated adults in urban north India about inhaled therapies","authors":"Khushboo Verma , Amit Misra","doi":"10.1016/j.tube.2024.102591","DOIUrl":"10.1016/j.tube.2024.102591","url":null,"abstract":"<div><div>We surveyed 15 persons with a medical qualification, 133 graduate students doing biomedical research and 56 students or working people with a college education in any discipline. Questions were designed to gauge awareness about inhaled therapies for tuberculosis (TB), non-tubercular mycobacterial lung disease (NTM-LD) and idiopathic pulmonary fibrosis (IPF). Respondents from six cities in North India, aged between 21 and 57 years answered 20 questions. All physicians, 99.25 % of graduate students and 85.71 % of the rest were aware and positive about inhalations for asthma, but these proportions fell to 69.92 and 66.07 in respect of other diseases. All respondents in the first two categories agreed that it was easy to train patients in the use of inhalation devices, while the third group was unanimous that there would be no aversion to using inhalation devices. A question asking whether the respondent would prescribe or opt for inhaled therapies for own use elicited an affirmative answer only from 40.00 % of physicians, 43.61 % of researchers and 23.21 % of college-educated persons (overall: 37.56 %). We concluded that inhaled therapies for diseases other than asthma are not well known and find limited acceptance among the populations sampled.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"150 ","pages":"Article 102591"},"PeriodicalIF":2.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0