单剂结核病疫苗接种中卡介苗的微针介导皮内递送。

IF 2.8 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2025-01-16 DOI:10.1016/j.tube.2025.102608

Sanha Lee , Taeyoon Kim , Keum-Yong Seong , Sang-Gu Yim , Won-Kyu Lee , Semin Kim , Kang-Oh Lee , Seung Yun Yang , Sungweon Ryoo

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引用次数: 0

摘要

结核病（TB）仍然是一种高度致命的传染病。主要预防措施是卡介苗，一种减毒活疫苗。然而，目前使用10剂小瓶皮内疫苗接种方法面临着婴儿注射不足、配药不准确和储存不稳定等挑战。研究人员已经探索了微针（MN）技术作为一种透皮疫苗递送方法来解决这些问题。MNs提供无痛给药、方便、改进的免疫原性和疫苗稳定性。本研究旨在利用低温（4°C）微分配技术和特定赋形剂开发一种包被MN系统，以精确给药和提高疫苗活力。长期储存稳定性表明，bcg包被MN （BCG-MN）疫苗的储存稳定性增强，在-20°C下维持8周的存活率超过60%。在豚鼠体内，使用BCG-MN贴片进行的疫苗接种试验未显示出不良反应。此外，与注射相比，BCG-MN疫苗贴片显示出更好的免疫应答，这表明这种BCG疫苗包被MN平台具有作为单剂量结核病疫苗接种技术的潜力，提供精确的剂量控制和增强的免疫效果，并具有高储存稳定性。

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Microneedle-mediated intradermal delivery of Bacille Calmette-Guérin (BCG) vaccines for single-dose tuberculosis vaccination

Tuberculosis (TB) remains a highly lethal infectious disease. The primary preventive measure is Bacille Calmette-Guérin (BCG), a live attenuated vaccine. However, the current intradermal vaccination method with 10-dose vials faces challenges such as inadequate infant injection, inaccurate dispensing, and unstable storage. Researchers have explored microneedle (MN) technology to address these concerns as a intradermal vaccine delivery approach. MN array patches offer painless administration, convenience, improved immunogenicity, and vaccine stability. This study aimed to develop a coated MN system using a micro-dispensing technique at a low temperature (4 °C) and specific excipients for precise dosing and vaccine viability enhancement. Long-term storage stability revealed enhanced storage stability of the BCG-coated MN (BCG-MN) vaccine, maintaining a survival rate of over 60 % for 8 weeks at −20 °C. In vivo vaccination tests using BCG-MN vaccines on guinea pigs exhibited no adverse reactions. Moreover, the BCG-MN vaccine demonstrated superior immune response compared to injections, suggesting that this BCG vaccine-coated MN platform has the potential as a single-dose TB vaccination technology, offering precise dosing control and enhanced immune effectiveness with high storage stability.

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.