Tuberculosis最新文献_第4页

Optimization of Mycobacterium tuberculosis DNA processing prior to whole genome sequencing 优化全基因组测序前的结核分枝杆菌 DNA 处理。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-07-11 DOI: 10.1016/j.tube.2024.102543

Samira Dziri , Julie Marin , Pauline Quagliaro , Charlotte Genestet , Oana Dumitrescu , Etienne Carbonnelle , Typhaine Billard-Pomares

{"title":"Optimization of Mycobacterium tuberculosis DNA processing prior to whole genome sequencing","authors":"Samira Dziri , Julie Marin , Pauline Quagliaro , Charlotte Genestet , Oana Dumitrescu , Etienne Carbonnelle , Typhaine Billard-Pomares","doi":"10.1016/j.tube.2024.102543","DOIUrl":"10.1016/j.tube.2024.102543","url":null,"abstract":"<div><p>The process of whole genome sequencing of the <em>Mycobacterium tuberculosis complex</em> is dependent on complete the inactivation of the strain and subsequent DNA extraction. The objective of this study was to optimise the two steps.</p><p>Firstly, the efficacy of Triton X-100 as a solvent for the inactivation step was evaluated. This solvent has been demonstrated to be effective in killing bacteria and is less toxic than the previously employed chloroform. For the extraction step, two lysis methods were evaluated: enzymatic (B1 protocol) and mechanical (B2 protocol). For whole genome sequencing, the Nextera XT DNA library preparation protocol was performed for both the B1 and B2 protocols. Subsequently, each library was subjected to whole-genome sequencing. The results demonstrated that heat lysis inactivation with Triton was effective, with no bacteria remaining viable following this treatment. The enzymatic and mechanical extraction protocols yielded comparable results in terms of DNA quantity and quality. The sequencing results showed that there was no significant difference in read depths between the two protocols. In conclusion, for MTBC strains, we recommend the use of our Triton inactivation method, which meets biosafety expectations.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102543"},"PeriodicalIF":2.8,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000696/pdfft?md5=3ca678ea5eb9e059ca535362ab3a5edd&pid=1-s2.0-S1472979224000696-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141621005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced efficacy of BCG vaccine formulated in adjuvant is dependent on IL-17A expression 用佐剂配制的卡介苗疗效的提高取决于 IL-17A 的表达。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-07-06 DOI: 10.1016/j.tube.2024.102540

Steven C. Derrick, Amy Yang, Siobhan Cowley

{"title":"Enhanced efficacy of BCG vaccine formulated in adjuvant is dependent on IL-17A expression","authors":"Steven C. Derrick, Amy Yang, Siobhan Cowley","doi":"10.1016/j.tube.2024.102540","DOIUrl":"10.1016/j.tube.2024.102540","url":null,"abstract":"<div><p>A new, more effective vaccine against tuberculosis (TB) is urgently needed to curtail the current TB problem. The only licensed vaccine, BCG, has been shown to have highly variable protective efficacy in several clinical trials ranging from zero to 80 % against TB disease. We have previously reported that BCG formulated in dimethyl dioctadecyl-ammonium bromide (DDA) with D-(+)-Trehalose 6,6′-Dibehenate (TDB) adjuvant (BCG + Adj) is significantly more protective than BCG alone following murine aerosol <em>Mycobacterium tuberculosis</em> infection. Here we investigate the immunological basis for this improved efficacy by examining expression of different immune markers and cytokines in the lungs of vaccinated mice after <em>M. tuberculosis</em> aerosol challenge. We found significantly greater numbers of pulmonary IL-17A-expressing CD4<sup>+</sup> T cells in mice immunized with BCG+Adj as compared to nonvaccinated and BCG-immunized mice at one-month post-challenge and that the enhanced protection was abrogated in IL-17A-deficient mice. Furthermore, we found significantly higher levels of IL-17A, IL-12p40 and IL-33 expression in the lungs of BCG + Adj immunized animals relative to nonvaccinated mice after <em>M. tuberculosis</em> challenge. These results demonstrate that the DDA/TDB adjuvant increases expression of IL-17A in response to the BCG vaccine and that these augmented IL-17A levels enhance control of <em>M. tuberculosis</em> infection.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102540"},"PeriodicalIF":2.8,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteome overview of exosome derived from plasma of cows infected with Mycobacterium bovis 从感染了牛分枝杆菌的奶牛血浆中提取的外泌体蛋白质组概览。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-07-06 DOI: 10.1016/j.tube.2024.102541

Hangfan Zhou , Wenhui Wu , Qilong Zhang , Tao Zhang , Songhao Jiang , Lei Chang , Yuping Xie , Jiaqiang Zhu , Degang Zhou , Yao Zhang , Ping Xu

{"title":"Proteome overview of exosome derived from plasma of cows infected with Mycobacterium bovis","authors":"Hangfan Zhou , Wenhui Wu , Qilong Zhang , Tao Zhang , Songhao Jiang , Lei Chang , Yuping Xie , Jiaqiang Zhu , Degang Zhou , Yao Zhang , Ping Xu","doi":"10.1016/j.tube.2024.102541","DOIUrl":"10.1016/j.tube.2024.102541","url":null,"abstract":"<div><p>Bovine tuberculosis (bTB), primarily caused by <em>Mycobacterium bovis</em> (<em>M. bovis</em>), is a globally zoonotic disease with significant economic impacts. Plasma exosomes have been extensively used for investigating disease processes and exploring biomarkers. While mass spectrometry (MS)-based proteomic analysis of plasma exosomes has been employed for human tuberculosis (TB) studies, it has not yet been applied to bTB. Therefore, a comprehensive proteomic overview of plasma exosomes from <em>M. bovis</em>-infected cows is essential. In this study, we presented an extensive proteomic analysis of plasma exosomes from 89 <em>M. bovis</em>-infected cows across three farms, using data dependent acquisition (DDA) mode. Our analysis encompasses 239,894 spectra, 6,011 peptides and 835 proteins. The proteomic overview revealed both consistencies and differences among individual cows, supplements 595 proteins to the bovine exosome library, and enriches tuberculosis and related pathways. Additionally, six pathways were validated as immune response pathways, and three proteins (CATHL1, H1-1, and LCN2) were identified as potential indicators of bTB. This study is the first to investigate the exosome proteome of plasma from cows infected with <em>M. bovis</em>, providing a valuable dataset for exploring candidate bTB markers and understanding the mechanisms of host defense against <em>M. bovis</em>.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102541"},"PeriodicalIF":2.8,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of host immune-related biomarkers in active tuberculosis: A comprehensive analysis of differentially expressed genes 活动性结核病宿主免疫相关生物标志物的鉴定：全面分析差异表达基因

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-06-28 DOI: 10.1016/j.tube.2024.102538

Alisha Ansari , Gajendra Pratap Singh , Mamtesh Singh , Harpreet Singh

{"title":"Identification of host immune-related biomarkers in active tuberculosis: A comprehensive analysis of differentially expressed genes","authors":"Alisha Ansari , Gajendra Pratap Singh , Mamtesh Singh , Harpreet Singh","doi":"10.1016/j.tube.2024.102538","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102538","url":null,"abstract":"<div><p>Tuberculosis (TB) is a serious public health issue in India. Numerous molecular mechanisms and immunological responses play significant roles in the pathogenesis of tuberculosis. This study aimed to identify host immune-related biomarkers that are significantly differentially expressed in active TB and that play a vital role in disease progression. The methodology employed in this study included data collection, pre-processing, analysis, and interpretation of the results. Six microarray datasets were used to identify differentially expressed genes (DEGs), and only the common DEGs were used for further downstream analysis, such as hub gene identification, gene ontology, pathway enrichment analysis, and drug-gene interaction analysis. The study identified 1728 DEGs, including 906 upregulated and 822 downregulated genes. Five hub genes were identified that were: STAT1, GBP5, GBP1, FCGR1A, and BATF2. Gene ontology and pathway enrichment revealed that most of the genes were involved in interferon-gamma signaling. In addition, through drug-gene interactions, known drugs have been identified for STAT1, FCGR1A and GBP1. The findings of this study may contribute to early detection and treatment of active TB.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102538"},"PeriodicalIF":2.8,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis 在 QFT-TB 检测中同时检测宿主 T 细胞和 NK 细胞上的 CD69 和 HLA-DR 对识别活动性结核病的鉴别诊断价值

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-06-27 DOI: 10.1016/j.tube.2024.102537

Yiqi Yang , Fujie Zhang , Hanlu Shi , Zhongliang Zhu , Yu Zhou , Yonglie Zhou

{"title":"Differential diagnostic value of simultaneous detection of CD69 and HLA-DR on host T and NK cells in QFT-TB assay for identifying active tuberculosis","authors":"Yiqi Yang , Fujie Zhang , Hanlu Shi , Zhongliang Zhu , Yu Zhou , Yonglie Zhou","doi":"10.1016/j.tube.2024.102537","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102537","url":null,"abstract":"<div><h3>Background</h3><p>Interferon-gamma release assay (IGRA) for tuberculosis (TB) remains limited in its ability to discriminate between active TB (ATB) and latent TB infection (LTBI). Activation markers on host T and NK cells are currently considered to be promising markers in the diagnosis of ATB.</p></div><div><h3>Methods</h3><p>This prospective observational study enrolled 213 participants and the participants were divided into ATB, LTBI, other lung-related diseases (ORD), and health control (HC) groups. CD69 and HLA-DR on T and NK cells were detected in QFT-TB assay, and a composite scoring system (TB-Flow) was created for the diagnosis of ATB.</p></div><div><h3>Results</h3><p>The expression of activation markers (CD69 and HLA-DR) were significantly increased in ATB. HLA-DR on NK cells, CD69 on T cells, and QFT-TB in the differential diagnosis of ATB and HC were all of good diagnostic value (AUC>0.90). In addition, the TB-Flow greatly improved the efficiency of differential diagnosis between ATB and LTBI (AUC=0.90, 95%CI: 0.84–0.96), with sensitivity and specificity of 79.17 % (95%CI: 64.60%–89.04 %) and 88.68 % (95%CI: 76.28%–95.31 %).</p></div><div><h3>Conclusions</h3><p>CD69 and HLA-DR on host T and NK cells are promising markers in distinguishing different TB infection status. Our blood-based TB-Flow scoring system can distinguish ATB from LTBI with good diagnostic efficacy.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102537"},"PeriodicalIF":2.8,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000635/pdfft?md5=dddeacf5d6042df0da651f3460859831&pid=1-s2.0-S1472979224000635-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141481847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isoniazid-induced pancreatitis: A systematic review 异烟肼诱发的胰腺炎：系统综述。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-06-25 DOI: 10.1016/j.tube.2024.102535

Tejaswini Baral , Sonal Sekhar M , Levin Thomas , Roopa Acharya B , Keerthana Krishnan , Sahana Shetty , Mahadev Rao

{"title":"Isoniazid-induced pancreatitis: A systematic review","authors":"Tejaswini Baral , Sonal Sekhar M , Levin Thomas , Roopa Acharya B , Keerthana Krishnan , Sahana Shetty , Mahadev Rao","doi":"10.1016/j.tube.2024.102535","DOIUrl":"10.1016/j.tube.2024.102535","url":null,"abstract":"<div><h3>Background</h3><p>Isoniazid-induced pancreatitis is a potentially serious adverse drug reaction, however, the frequency of its occurrence is unknown. We conducted a systematic review to explore this adverse drug reaction comprehensively.</p></div><div><h3>Methods</h3><p>We performed an advanced search in PubMed, Web of Science, Scopus, Ovid, and Embase for studies that reported isoniazid-induced pancreatitis. From the extracted data of eligible cases, we performed a descriptive analysis and a methodological risk of bias assessment using a standardized tool.</p></div><div><h3>Results</h3><p>We included 16 case reports from eight countries comprising 16 patients in our systematic review. Most of the isoniazid-induced pancreatitis cases were extrapulmonary tuberculosis cases. We found the mean age across all case reports was 36.7 years. In all the cases, discontinuation of isoniazid resulted in the resolution of pancreatitis.</p></div><div><h3>Conclusions</h3><p>We found the latency period for isoniazid-induced pancreatitis to be ranged from 12 to 45 days after initiation of isoniazid therapy. A low threshold for screening of pancreatitis by measuring pancreatic enzymes in patients on isoniazid presenting with acute abdominal pain is recommended. This would facilitate an early diagnosis and discontinuation of isoniazid, thus reducing the severity of pancreatitis and preventing the complications of pancreatitis.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102535"},"PeriodicalIF":2.8,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000611/pdfft?md5=7356043d5790aae81b9ba038ae0a4cdf&pid=1-s2.0-S1472979224000611-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141470953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage-targeted versus free calcitriol as host-directed adjunct therapy against Mycobacterium tuberculosis infection in mice is bacteriostatic and mitigates tissue pathology 以巨噬细胞为靶点的降钙素三醇与游离降钙素三醇相比，作为宿主导向的辅助疗法，对小鼠结核分枝杆菌感染具有抑菌作用，并能减轻组织病理变化。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-06-25 DOI: 10.1016/j.tube.2024.102536

D.V. Siva Reddy , Hasham Shafi Sofi , Trisha Roy , Sonia Verma , Kaveri R. Washimkar , Sunil Kumar Raman , Sanjay Singh , Lubna Azmi , Lipika Ray , Jyotsna Singh , Madhav N. Mugale , Amit K. Singh , Amit Misra

{"title":"Macrophage-targeted versus free calcitriol as host-directed adjunct therapy against Mycobacterium tuberculosis infection in mice is bacteriostatic and mitigates tissue pathology","authors":"D.V. Siva Reddy , Hasham Shafi Sofi , Trisha Roy , Sonia Verma , Kaveri R. Washimkar , Sunil Kumar Raman , Sanjay Singh , Lubna Azmi , Lipika Ray , Jyotsna Singh , Madhav N. Mugale , Amit K. Singh , Amit Misra","doi":"10.1016/j.tube.2024.102536","DOIUrl":"10.1016/j.tube.2024.102536","url":null,"abstract":"<div><p>Host-directed therapy (HDT) with vitamin D in tuberculosis (TB) is beneficial only if the subject is deficient in vitamin D. We investigated pulmonary delivery of 1,25-dihydroxy vitamin D<sub>3</sub> (calcitriol) in mice infected with <em>Mycobacterium tuberculosis</em> (Mtb). We made two kinds of dry powder inhalations (DPI)— soluble particles or <em>poly</em>(lactide) (PLA) particles. We compared treatment outcomes when infected mice were dosed with a DPI alone or as an adjunct to standard oral anti-TB therapy (ATT). Mice infected on Day 0 were treated between Days 28–56 and followed up on Days 57, 71, and 85. Neither DPI significantly reduced Mtb colony forming units (CFU) in the lungs. Combining DPI with ATT did not significantly augment bactericidal activity in the lungs, but CFU were 2-log lower in the spleen. CFU showed a rising trend on stopping treatment, sharper in groups that did not receive calcitriol. Lung morphology and histology improved markedly in animals that received PLA DPI; with or without concomitant ATT. Groups receiving soluble DPI had high mortality. DPI elicited cathelicidin, interleukin (IL)-1 and induced autophagy on days 57, 71, and 85. Macrophage-targeted calcitriol is therefore bacteriostatic, evokes innate microbicidal mechanisms, and mitigates pathology arising from the host response to Mtb.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102536"},"PeriodicalIF":2.8,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141559787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic investigation of bone tuberculosis highlighted the role of subclinical pulmonary tuberculosis in transmission 骨结核基因组调查凸显亚临床肺结核在传播中的作用

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-06-13 DOI: 10.1016/j.tube.2024.102534

Jinfeng Yin , Guangxuan Yan , Liyi Qin , Chendi Zhu , Jun Fan , Yuwei Li , Junnan Jia , Zhaojun Wu , Hui Jiang , Muhammad Tahir Khan , Jiangdong Wu , Naihui Chu , Howard E. Takiff , Qian Gao , Shibing Qin , Qingyun Liu , Weimin Li

{"title":"Genomic investigation of bone tuberculosis highlighted the role of subclinical pulmonary tuberculosis in transmission","authors":"Jinfeng Yin , Guangxuan Yan , Liyi Qin , Chendi Zhu , Jun Fan , Yuwei Li , Junnan Jia , Zhaojun Wu , Hui Jiang , Muhammad Tahir Khan , Jiangdong Wu , Naihui Chu , Howard E. Takiff , Qian Gao , Shibing Qin , Qingyun Liu , Weimin Li","doi":"10.1016/j.tube.2024.102534","DOIUrl":"10.1016/j.tube.2024.102534","url":null,"abstract":"<div><h3>Background</h3><p>Extrapulmonary tuberculosis (EPTB) without symptomatic pulmonary involvement has been thought to be non-transmissible, but EPTB with asymptomatic pulmonary tuberculosis (PTB) could transmit tuberculosis (TB). Genomic investigation of <em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) isolates from EPTB may provide insight into its epidemiological role in TB transmission.</p></div><div><h3>Methods</h3><p>Between January 2017 and May 2020, 107 Mtb isolates were obtained from surgical drainage of bone TB patients at the Beijing Chest Hospital, and 218 Mtb strains were isolated from PTB cases. These 325 Mtb isolates were whole-genome sequenced to reconstruct a phylogenetic tree, identify transmission clusters, and infer transmission links using a Bayesian approach. Possible subclinical PTB in the bone TB patients was investigated with chest imaging by two independent experts.</p></div><div><h3>Results</h3><p>Among 107 bone TB patients, 10 were in genomic clusters (≤12 SNPs). Phylogenetic analysis suggested that three bone TB patients transmitted the infection to secondary cases, supported by epidemiological investigations. Pulmonary imaging of 44 bone TB patients revealed that 79.5 % (35/44) had radiological abnormalities suggestive of subclinical PTB.</p></div><div><h3>Conclusions</h3><p>This study provides genomic evidence that bone TB patients without clinically diagnosed PTB can be sources of TB transmission, underscoring the importance of screening for subclinical, transmissible PTB among EPTB cases.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102534"},"PeriodicalIF":2.8,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141394853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of immune profiles associated with control of mycobacterial growth in systemic lupus erythematosus (SLE) patients 评估与控制系统性红斑狼疮（SLE）患者分枝杆菌生长有关的免疫特征

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-06-13 DOI: 10.1016/j.tube.2024.102533

Jomkwan Ongarj , Porntip Intapiboon , Smonrapat Surasombatpattana , Iman Satti , Stephanie A. Harris , Hazel Morrison , Ratchanon Sophonmanee , Helen McShane , Rachel Tanner , Nawamin Pinpathomrat

{"title":"Evaluation of immune profiles associated with control of mycobacterial growth in systemic lupus erythematosus (SLE) patients","authors":"Jomkwan Ongarj , Porntip Intapiboon , Smonrapat Surasombatpattana , Iman Satti , Stephanie A. Harris , Hazel Morrison , Ratchanon Sophonmanee , Helen McShane , Rachel Tanner , Nawamin Pinpathomrat","doi":"10.1016/j.tube.2024.102533","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102533","url":null,"abstract":"<div><p>Tuberculosis (TB) is an infectious disease with the burden concentrated in low- and middle-income countries. Systemic lupus erythematosus (SLE) is an autoimmune disease associated with widespread inflammation that is prevalent in some TB endemic areas including East Africa and parts of Southeast Asia. SLE patients are known to be at higher risk of becoming infected with <em>M. tb</em>, developing TB disease. However, the immune mechanisms underlying this susceptibility are not well understood, particularly in the absence of immunosuppressive drugs. We present a pilot study in which we have evaluated intracellular cytokine responses and <em>ex vivo</em> ability to control mycobacterial growth using peripheral blood mononuclear cells (PBMC) collected from SLE patients before and during SLE treatment. After six months of treatment, SLE patients had the highest frequencies of CD8<sup>+</sup> T cells, NK cells and NKT cells producing IFN-γ and/or TNF-α. This group also showed superior control of mycobacterial growth, and proinflammatory cytokine-producing NK and NKT cells correlated with mycobacterial growth inhibition at the individual patient level. These findings contribute to a better understanding of autoimmune profiles associated with control of mycobacterial growth in SLE patients, which may inform intervention strategies to reduce risk of TB disease in this population.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102533"},"PeriodicalIF":3.2,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141323181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A blood-based 3-gene signature score for therapeutic monitoring in patients with pulmonary tuberculosis: Correspondence 用于肺结核患者治疗监测的基于血液的 3 基因特征评分：通讯

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-06-11 DOI: 10.1016/j.tube.2024.102532

Hinpetch Daungsupawong, Viroj Wiwanitkit

引用次数: 0