Tuberculosis最新文献_第5页

Synergistic role of Mycobacterium indicus pranii and human beta Defensin-2 as adjunctive therapy against Mycobacterium tuberculosis 吲哚分枝杆菌和人类 beta Defensin-2 作为结核分枝杆菌辅助疗法的协同作用。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-10-15 DOI: 10.1016/j.tube.2024.102571

Shah Faisal , Deepak Vats , Sudhasini Panda , Vidushi Sharma , Kalpana Luthra , Anant Mohan , Savita Kulkarni , Pramod Kumar Gupta , Archana Singh

{"title":"Synergistic role of Mycobacterium indicus pranii and human beta Defensin-2 as adjunctive therapy against Mycobacterium tuberculosis","authors":"Shah Faisal , Deepak Vats , Sudhasini Panda , Vidushi Sharma , Kalpana Luthra , Anant Mohan , Savita Kulkarni , Pramod Kumar Gupta , Archana Singh","doi":"10.1016/j.tube.2024.102571","DOIUrl":"10.1016/j.tube.2024.102571","url":null,"abstract":"<div><div>Host-directed therapies (HDT) via modulation of specific host responses like inflammation can limit mycobacterial infection. HDTs could be included in current TB therapy as an adjunct to increase bacterial clearance and limit tissue damage to control spread. Individually, <em>Mycobacterium indicus pranii (MIP)</em> and human beta defensin-2 (hBD-2) are promising therapies for tuberculosis (TB). They can directly target the TB bacilli and enhance cell-mediated immune responses, which is limiting with conventional drugs. Therefore, our study investigated the combined application of MIP and hBD-2 to evaluate their efficacy in clearing infections caused by <em>Mycobacterium smegmatis</em> (<em>M.</em><em>smeg</em>) and <em>Mycobacterium tuberculosis (M</em>.<em>tb)</em> (both avirulent; H37Ra and virulent strain; H37Rv) in THP-1 cells and human monocyte-derived macrophages (MDMs). A strong pro-inflammatory response was observed against the combination of MIP and hBD-2 which also correlated with a significant reduction in the bacterial load. This combination further showed protection against <em>M.tb</em> by enhancing pyroptosis in the infected cells. The study suggests the combined use of these potent immunomodulators, which could be employed as an effective mode of therapy as adjuvants against mycobacterial infections after validation in a suitable animal model.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102571"},"PeriodicalIF":2.8,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining bioinformatics and machine learning to identify diagnostic biomarkers of TB associated with immune cell infiltration 结合生物信息学和机器学习，确定与免疫细胞浸润相关的结核病诊断生物标志物

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-10-11 DOI: 10.1016/j.tube.2024.102570

Shoupeng Ding , Xiaomei Yi , Jinghua Gao , Chunxiao Huang , Yuyang Zhou , Yimei Yang , Zihan Cai

{"title":"Combining bioinformatics and machine learning to identify diagnostic biomarkers of TB associated with immune cell infiltration","authors":"Shoupeng Ding , Xiaomei Yi , Jinghua Gao , Chunxiao Huang , Yuyang Zhou , Yimei Yang , Zihan Cai","doi":"10.1016/j.tube.2024.102570","DOIUrl":"10.1016/j.tube.2024.102570","url":null,"abstract":"<div><h3>Objective</h3><div>The asymptomatic nature of tuberculosis (TB) during its latent phase, combined with limitations in current diagnostic methods, makes accurate diagnosis challenging. This study aims to identify TB diagnostic biomarkers by integrating gene expression screening with machine learning, evaluating their diagnostic potential and correlation with immune cell infiltration.</div></div><div><h3>Methods</h3><div>We analyzed GSE19435, GSE19444, and GSE54992 datasets to identify differentially expressed genes (DEGs). GO and KEGG enrichment characterized gene functions. Three machine learning algorithms identified potential biomarkers, validated with GSE83456, GSE62525, and RT-qPCR on clinical samples. Immune cell infiltration was analyzed and verified with blood data.</div></div><div><h3>Results</h3><div>249 DEGs were identified, with PDE7A and DOK3 emerging as potential biomarkers. RT-qPCR confirmed their expression, showing AUCs above 0.75 and a combined AUC of 0.926 for TB diagnosis. Immune infiltration analysis revealed strong correlations between PDE7A, DOK3, and immune cells.</div></div><div><h3>Conclusion</h3><div>PDE7A and DOK3 show strong diagnostic potential for TB, closely linked to immune cell infiltration, and may serve as promising biomarkers and therapeutic targets.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102570"},"PeriodicalIF":2.8,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel C-4-modified isotetrone acts as a potent bio-enhancer to augment the activities of anti-tuberculosis drugs against Mycobacterium tuberculosis 一种新型 C-4 改性异四氢呋喃可作为一种有效的生物增强剂，增强抗结核药物对结核分枝杆菌的活性。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-09-27 DOI: 10.1016/j.tube.2024.102569

Naveen Prakash Bokolia , Kingshuk Bag , Biplab Sarkar , Ruchi Jhawar , Dipankar Chatterji , Narayanaswamy Jayaraman , Anirban Ghosh

{"title":"A novel C-4-modified isotetrone acts as a potent bio-enhancer to augment the activities of anti-tuberculosis drugs against Mycobacterium tuberculosis","authors":"Naveen Prakash Bokolia , Kingshuk Bag , Biplab Sarkar , Ruchi Jhawar , Dipankar Chatterji , Narayanaswamy Jayaraman , Anirban Ghosh","doi":"10.1016/j.tube.2024.102569","DOIUrl":"10.1016/j.tube.2024.102569","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> is a deadly pathogen that claims millions of lives every year. Current research focuses on finding new anti-tuberculosis drugs that are safe and effective, with lesser side effects and toxicity. One important approach is to identify bio-enhancers that can improve the effectiveness of anti-tuberculosis drugs, resulting in reduced doses and shortened treatment times. The present study investigates the use of <em>C</em>-4 modified isotetrones as bio-enhancers. A series of studies suggest an isotetrone, labeled as C11, inhibits growth, improves MIC, MBC and enhances the killing of <em>M. tuberculosis</em> H37Rv strain when used in combination with the first line and injectable anti-TB drugs in a dose-dependent manner. The combination of C11 and rifampicin also reduces the generation of spontaneous mutants against rifampicin and reaches a mutation prevention concentration (MPC) with moderate rifampicin concentrations. The identified compounds are effective against the MDR strain of <em>M. tuberculosis</em> and non-cytotoxic in HepG2 cells. We find that C11 induces the generation of reactive oxygen species (ROS) inside macrophages and within bacteria, resulting in better efficacy.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102569"},"PeriodicalIF":2.8,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142366685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuberculosis and T cells: Impact of T cell diversity in tuberculosis infection 结核病与 T 细胞结核感染中 T 细胞多样性的影响

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-09-18 DOI: 10.1016/j.tube.2024.102567

Deepak Vats, Geeta Rani, Alisha Arora, Vidushi Sharma, Isha Rathore, Shaikh Abdul Mubeen, Archana Singh

{"title":"Tuberculosis and T cells: Impact of T cell diversity in tuberculosis infection","authors":"Deepak Vats, Geeta Rani, Alisha Arora, Vidushi Sharma, Isha Rathore, Shaikh Abdul Mubeen, Archana Singh","doi":"10.1016/j.tube.2024.102567","DOIUrl":"10.1016/j.tube.2024.102567","url":null,"abstract":"<div><p>Tuberculosis is a global threat and is still a leading cause of death due to an infectious agent. The infection is spread through inhalation of <em>M. tb</em> containing aerosol droplets. Bacteria after reaching the lung alveoli are engulfed by alveolar macrophages, leading to an immune response. Then, pro-inflammatory cytokines are released by these macrophages, recruiting other antigen-presenting cells like dendritic cells. These cells phagocytose the bacteria and present mycobacterial antigens to naïve T cells. After activation by DCs, T cells differentiate into various T cells subsets, viz. CD4<sup>+</sup>, CD8<sup>+</sup>, Th17, Treg, Tfh cells and others display enormous diversification in their characteristics and functions. This review comprises a comprehensive literature on conventional and unconventional T cells, highlighting the polyfunctional T cells as well, their role in controlling TB infection, and their implications in the spectrum of TB infection. While some subsets such as CD4<sup>+</sup> T cells are extensively studied, some T cell subsets such as gamma delta T cells and Tfh cells remain poorly understood in the pathophysiology of tuberculosis, despite having significant potential implications. The goal of TB eradication can be assisted by development of better vaccines against TB, which can effectively induce a robust and long-term T cells memory. The same has been discussed in the latter part of this review. BCG being the standalone commercialised TB vaccine so far has its limitations. Strategies for the enhancement of BCG along with novel studies in vaccine development, has also been discussed in great detail. Lastly, T cells display a complex interplay of an adaptive immune response against TB, with activation and enhancement of the innate immune responses. Therefore, it is critical to fully understand the role of various T cells subsets in pathophysiology of tuberculosis to provide better therapeutic inventions and improve patient care.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102567"},"PeriodicalIF":2.8,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A description of lineage 1 Mycobacterium tuberculosis from papua, Indonesia 印度尼西亚巴布亚地区结核分枝杆菌 1 系的描述

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-09-14 DOI: 10.1016/j.tube.2024.102568

Hanif A.K. Djunaedy , Clarissa A. Febinia , Raph L. Hamers , Kevin Baird , Iqbal Elyazar , Nguyen Thuy Thuong Thuong , Hidayat Trimarsanto , Safarina G. Malik , Guy Thwaites , Reinout van Crevel , Bachti Alisjahbana , Lidya Chaidir , Philip M. Ashton

{"title":"A description of lineage 1 Mycobacterium tuberculosis from papua, Indonesia","authors":"Hanif A.K. Djunaedy , Clarissa A. Febinia , Raph L. Hamers , Kevin Baird , Iqbal Elyazar , Nguyen Thuy Thuong Thuong , Hidayat Trimarsanto , Safarina G. Malik , Guy Thwaites , Reinout van Crevel , Bachti Alisjahbana , Lidya Chaidir , Philip M. Ashton","doi":"10.1016/j.tube.2024.102568","DOIUrl":"10.1016/j.tube.2024.102568","url":null,"abstract":"<div><div>Indonesia has the third highest number of tuberculosis (TB) patients infected with Mycobacterium tuberculosis (<em>MTB</em>) Lineage 1 (L1). Most of these MTB L1 cases can be found in Indonesia's remote easternmost province of Papua, one of Indonesia's most underdeveloped provinces with a particularly high burden for TB. In this study, we sequenced and described 42 <em>MTB</em> L1 isolates from a well-characterized cohort of patients. We found a genetically diverse <em>MTB</em> L1 population with no association between pathogen genetic relatedness and place of residence or pathogen genetic relatedness and patient ethnicity, which could reflect mixing between different locales and ethnicities or our low sampling fraction. Only a small number showed genetic variants associated with drug resistance (5/42, 11.9 %), probably due to a lack of effective treatment programs. The Papuan isolates showed similarities to other Island Southeast Asian Countries due to the high proportion of L1.2.1.2.1 (30/42, 71.4 %), especially East Timor and the Philippines. This study fills a research gap of MTB L1 in Indonesian Papua and should serve as a stepping stone for further research in the region.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102568"},"PeriodicalIF":2.8,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000945/pdfft?md5=a6f4d495c84889622c44816e926c96f8&pid=1-s2.0-S1472979224000945-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142315327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exhaled breath analysis: A promising triage test for tuberculosis in young children 呼气分析：有望成为幼儿结核病分诊测试的方法

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-09-10 DOI: 10.1016/j.tube.2024.102566

Else M. Bijker , Jonathan P. Smith , Walter Mchembere , Kimberly D. McCarthy , Henny Oord , Jan-Willem Gerritsen , Eleanor S. Click , Kevin Cain , Rinn Song

{"title":"Exhaled breath analysis: A promising triage test for tuberculosis in young children","authors":"Else M. Bijker , Jonathan P. Smith , Walter Mchembere , Kimberly D. McCarthy , Henny Oord , Jan-Willem Gerritsen , Eleanor S. Click , Kevin Cain , Rinn Song","doi":"10.1016/j.tube.2024.102566","DOIUrl":"10.1016/j.tube.2024.102566","url":null,"abstract":"<div><div>The diagnosis of paediatric pulmonary tuberculosis is difficult, especially in young infants who cannot expectorate sputum spontaneously. Breath testing has shown promise in diagnosing respiratory tract infections, but data on paediatric tuberculosis are limited.</div><div>We performed a prospective cross-sectional study in Kenya in children younger than five years with symptoms of tuberculosis. We analysed exhaled breath with a hand-held battery-powered nose device. For data analysis, machine learning was applied using samples classified as positive (microbiologically confirmed) or negative (unlikely tuberculosis) to assess diagnostic accuracy.</div><div>Breath analysis was performed in 118 children. The area under the curve of the optimal model was 0.73. At a sensitivity of 86 % (CI 62–96 %), this resulted in a specificity of 42 % (95 % CI 30–55 %).</div><div>Exhaled breath analysis shows promise as a triage test for TB in young children, although the WHO target product characteristics were not met.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102566"},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of transcriptional markers for the differentiation of Mycobacterium mungi infection status in free-ranging banded mongoose (Mungos mungo) 评估转录标记以区分自由活动的带状金钱豹（Mungos mungo）的孟氏分枝杆菌感染状况

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-09-05 DOI: 10.1016/j.tube.2024.102565

Nicholas M. Sybertz , Shamim Al Jubaer , Michelle H. Larsen , Kathleen A. Alexander

{"title":"Assessment of transcriptional markers for the differentiation of Mycobacterium mungi infection status in free-ranging banded mongoose (Mungos mungo)","authors":"Nicholas M. Sybertz , Shamim Al Jubaer , Michelle H. Larsen , Kathleen A. Alexander","doi":"10.1016/j.tube.2024.102565","DOIUrl":"10.1016/j.tube.2024.102565","url":null,"abstract":"<div><p>There is an increasingly urgent need to improve our ability to accurately forecast and control zoonotic diseases in wildlife reservoirs. We are confronted, however, with the continued challenge of accurately determining host infection status across space and time. This dilemma is epitomized with the <em>Mycobacterium tuberculosis</em> Complex (MTBC) pathogens and particularly in free-ranging wildlife, a critical global challenge for both human and animal health. In humans, transcriptional markers have been increasingly identified as a robust tool for diagnosing <em>Mycobacterium tuberculosis</em> (<em>MTB</em>) infection status but have rarely been utilized for diagnosing TB in free-ranging wildlife populations. Here, we report the first use of transcriptional markers to evaluate TB infection status in a free-ranging wildlife species, banded mongoose (<em>Mungos mungo</em>), infected with the MTBC pathogen, <em>Mycobacterium mungi</em>. In this study, we found that <em>GBP5</em> and <em>DUSP3</em> were significantly upregulated in free-ranging banded mongoose infected with <em>M. mungi</em>. These results provide the first step in developing an antemortem diagnostic tool for use in free-ranging wildlife species. Our results highlight the potential of transcriptional marker-based assays to advance our ability to detect and manage TB in free-ranging wildlife, especially in field studies and other scenarios when conventional diagnostics are not feasible.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102565"},"PeriodicalIF":2.8,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing circulation of multi-drug resistant tuberculosis strains in Buryatia, high-burden and ethnically diverse region in the Russian Far East 俄罗斯远东地区的布里亚特（Buryatia）是结核病高发区和民族多样化地区，该地区的耐多药结核病菌株日益增多。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-08-30 DOI: 10.1016/j.tube.2024.102555

Igor Mokrousov , Maria Badleeva , Regina Mudarisova , Valery Kozhevnikov , Andrey Markhaev , Anastasia Guntupova , Anna Vyazovaya

{"title":"Increasing circulation of multi-drug resistant tuberculosis strains in Buryatia, high-burden and ethnically diverse region in the Russian Far East","authors":"Igor Mokrousov , Maria Badleeva , Regina Mudarisova , Valery Kozhevnikov , Andrey Markhaev , Anastasia Guntupova , Anna Vyazovaya","doi":"10.1016/j.tube.2024.102555","DOIUrl":"10.1016/j.tube.2024.102555","url":null,"abstract":"<div><p>Buryatia is a multidrug-resistant tuberculosis (MDR-TB) high-burden region in the Russian Far East with ethnically diverse population (30 % Mongoloid Buryats and 65 % Russians). Two hundred <em>M. tuberculosis</em> strains from newly-diagnosed patients were subjected to phenotypic testing and genotyping. The Beijing genotype was more prevalent among Russians than Buryats (68 % vs 53 %; P = 0.055). European non-Beijing genotypes (LAM, Ural, Haarlem) were double more prevalent in Buryats vs Russians (39.2 % vs 20.5 %; P = 0.01). Higher prevalence of Beijing among former prison inmates (79 % vs 61 % in other patients, P = 0.1) suggests its increased transmissibility. The Russian epidemic cluster B0/W148 was in 9.5 %, double smaller than elsewhere in Siberia. The hypervirulent Beijing 14717-15-cluster was endemic in Buryatia but paradoxically enough, it was more frequently isolated from Russians than Buryats (9.1 % vs 3.9 %; P = 0.2). Beijing subtypes B0/W148, CAO, and 14717-15 were associated with poly/multi-drug resistance (P = 0.01–0.0001). HIV coinfection was more frequent in Russians than in Buryats: 35/141 (24.8 %) vs 5/51 (9.8 %), P = 0.03. To conclude, <em>M. tuberculosis</em> population structure in Buryatia retained its singularities compared to other parts of Russia and remains strikingly different from the neighboring Mongolia. A circulation of strongly MDR-associated Beijing subtypes and drug-resistant non-Beijing strains highlights a risk of their broader dissemination.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"149 ","pages":"Article 102555"},"PeriodicalIF":2.8,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142146406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indole triazene compound TU112 demonstrates in vitro activity against dormant Mycobacterium tuberculosis and efficacy against chronic tuberculosis infection in mice 吲哚三嗪化合物 TU112 对休眠结核分枝杆菌具有体外活性，对小鼠慢性结核感染也有疗效

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-08-28 DOI: 10.1016/j.tube.2024.102556

Margarita O. Shleeva , Boris V. Nikonenko , Konstantin B. Majorov , Pavel Yu Ivanov , Alexander S. Apt , Valeria S. Velezheva

引用次数: 0

Detection of Mycobacterium tuberculosis complex in saliva by quantitative PCR: A potential alternative specimen for pulmonary tuberculosis diagnosis 通过定量 PCR 检测唾液中的结核分枝杆菌复合体：肺结核诊断的潜在替代样本。

IF 2.8 3区医学

Tuberculosis Pub Date : 2024-07-31 DOI: 10.1016/j.tube.2024.102554

Sosina Ayalew , Teklu Wegayehu , Binium Wondale , Dawit Kebede , Mahlet Osman , Sebsib Niway , Azeb Tarekegn , Bamlak Tessema , Stefan Berg , Roland T. Ashford , Adane Mihret

{"title":"Detection of Mycobacterium tuberculosis complex in saliva by quantitative PCR: A potential alternative specimen for pulmonary tuberculosis diagnosis","authors":"Sosina Ayalew , Teklu Wegayehu , Binium Wondale , Dawit Kebede , Mahlet Osman , Sebsib Niway , Azeb Tarekegn , Bamlak Tessema , Stefan Berg , Roland T. Ashford , Adane Mihret","doi":"10.1016/j.tube.2024.102554","DOIUrl":"10.1016/j.tube.2024.102554","url":null,"abstract":"<div><h3>Background</h3><p>Current tuberculosis (TB) diagnostic tests primarily rely on sputum samples, yet many TB patients cannot produce sputum. This study explored whether saliva could be used instead of sputum to diagnose pulmonary TB (PTB).</p></div><div><h3>Method</h3><p>The study included 32 patients with confirmed PTB and 30 patients with other respiratory diseases (ORD). Saliva from all study participants was subjected to quantitative (qPCR) assays targeting the IS<em>1081</em> gene for detection of <em>M. tuberculosis complex</em> DNA.</p></div><div><h3>Results</h3><p>The sensitivity of saliva IS<em>1081</em> qPCR was 65.6 % (95 % CI 48.4–80.2 %) with positive results for 21/32 PTB cases, while the specificity was 96.7 % (95 % CI 85.9–99.6 %) with negative results for 29/30 participants with ORD. Sensitivity improved to 72.4 % (95 % CI 54.6–86.0 %) when sputum-Xpert was used as the reference standard, while remaining similar at 65.5 % (95 % CI 47.4–80.7 %) when culture was used as the reference standard. In receiver operating characteristic (ROC) curve analysis, the area under the curve (AUC) for saliva IS<em>1081</em> qPCR was 82.5 % (95 % CI 71.7–93.3 %).</p></div><div><h3>Conclusion</h3><p>Saliva testing offers a promising alternative to sputum for TB diagnosis among confirmed PTB cases. Larger multicenter studies, encompassing diverse clinical TB characteristics, are needed to provide improved estimates of diagnostic sensitivity and specificity.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102554"},"PeriodicalIF":2.8,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0