Tuberculosis最新文献_第6页

Effect of Metformin on systemic chemokine responses during anti-tuberculosis chemotherapy 二甲双胍对抗结核化疗期间全身趋化因子反应的影响

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-06-01 DOI: 10.1016/j.tube.2024.102523

Nathella Pavan Kumar , Chandrasekaran Padmapriyadarsini , Arul Nancy , M. Tamizhselvan , Anant Mohan , Devarajulu Reddy , N. Poorana Ganga Devi , Prabakaran Rathinam , Bharathi Jeyadeepa , R.K. Shandil , Randeep Guleria , Manjula Singh , Subash Babu

{"title":"Effect of Metformin on systemic chemokine responses during anti-tuberculosis chemotherapy","authors":"Nathella Pavan Kumar , Chandrasekaran Padmapriyadarsini , Arul Nancy , M. Tamizhselvan , Anant Mohan , Devarajulu Reddy , N. Poorana Ganga Devi , Prabakaran Rathinam , Bharathi Jeyadeepa , R.K. Shandil , Randeep Guleria , Manjula Singh , Subash Babu","doi":"10.1016/j.tube.2024.102523","DOIUrl":"10.1016/j.tube.2024.102523","url":null,"abstract":"<div><h3>Background</h3><p>Metformin (MET), by boosting immunity, has been suggested as a host-adjunctive therapy to anti-tuberculosis treatment (ATT).</p></div><div><h3>Methods</h3><p>We evaluated whether adding MET to the standard ATT can alter the host chemokine response. We investigated the influence of metformin on the plasma levels of a wide panel of chemokines in a group of active tuberculosis patients before treatment, at 2nd month of ATT and at 6-months of ATT as part of our clinical study to examine the effect of metformin on ATT.</p></div><div><h3>Results</h3><p>Our results demonstrated that addition of metformin resulted in diminished CC (CCL1 and CCL3) and CXC (CXCL-2 and CXCL-10) chemokines in MET arm as compared to non-MET arm at the 2nd month and 6th month of ATT. In addition to this, MET arm showed significantly diminished chemokines in individuals with high bacterial burden and cavitary disease.</p></div><div><h3>Conclusion</h3><p>Our current data suggest that metformin alters chemokines responses that could potentially curb excessive inflammation during ATT.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102523"},"PeriodicalIF":3.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring diagnostic methods for drug-resistant tuberculosis: A comprehensive overview 探索耐药结核病的诊断方法：全面概述

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-31 DOI: 10.1016/j.tube.2024.102522

Andrea Sanchini , Alessio Lanni , Federico Giannoni , Alessandro Mustazzolu

{"title":"Exploring diagnostic methods for drug-resistant tuberculosis: A comprehensive overview","authors":"Andrea Sanchini , Alessio Lanni , Federico Giannoni , Alessandro Mustazzolu","doi":"10.1016/j.tube.2024.102522","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102522","url":null,"abstract":"<div><p>Despite available global efforts and funding, Tuberculosis (TB) continues to affect a considerable number of patients worldwide. Policy makers and stakeholders set clear goals to reduce TB incidence and mortality, but the emergence of multidrug-resistant TB (MDR-TB) and extensively drug-resistant TB (XDR-TB) complicate the reach of these goals. Drug-resistance TB needs to be diagnosed rapidly and accurately to effectively treat patients, prevent the transmission of MDR-TB, minimise mortality, reduce treatment costs and avoid unnecessary hospitalisations. In this narrative review, we provide a comprehensive overview of laboratory methods for detecting drug resistance in MTB, focusing on phenotypic, molecular and other drug susceptibility testing (DST) techniques. We found a large variety of methods used, with the BACTEC MGIT 960 being the most common phenotypic DST and the Xpert MTB/RIF being the most common molecular DST. We emphasise the importance of integrating phenotypic and molecular DST to address issues like resistance to new drugs, heteroresistance, mixed infections and low-level resistance mutations. Notably, most of the analysed studies adhered to the outdated definition of XDR-TB and did not consider the pre-XDR definition, thus posing challenges in aligning diagnostic methods with the current landscape of TB resistance.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"148 ","pages":"Article 102522"},"PeriodicalIF":3.2,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141285982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A blood-based 3-gene signature score for therapeutic monitoring in patients with pulmonary tuberculosis 用于肺结核患者治疗监测的基于血液的 3 基因特征评分

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-23 DOI: 10.1016/j.tube.2024.102521

Peize Zhang , Junfeng Zheng , Tingting Han , Jian Ma , Devasena Gnanashanmugam , Mengran Li , Yi-Wei Tang , Guofang Deng

{"title":"A blood-based 3-gene signature score for therapeutic monitoring in patients with pulmonary tuberculosis","authors":"Peize Zhang , Junfeng Zheng , Tingting Han , Jian Ma , Devasena Gnanashanmugam , Mengran Li , Yi-Wei Tang , Guofang Deng","doi":"10.1016/j.tube.2024.102521","DOIUrl":"10.1016/j.tube.2024.102521","url":null,"abstract":"<div><h3>Objective</h3><p>To assess the validity of Xpert Tuberculosis Fingerstick score for monitoring treatment response and analyze factors influencing its performance.</p></div><div><h3>Methods</h3><p>122 adults with pulmonary tuberculosis were recruited and stratified into three cohorts: Diabetic-drug-susceptible-TB (DM-TB), Non-diabetic-drug-susceptible-TB (NDM-TB) and Non-diabetic Multidrug-resistant TB (MDR-TB). Fingerstick blood specimens were tested at treatment initiation (M0) and the end of the first (M1), second (M2), and sixth month (M6) to generate a TB-score.</p></div><div><h3>Results</h3><p>The TB-score in all participants yielded an AUC of 0.707 (95% CI: 0.579–0.834) at M2 when its performance was evaluated against sputum culture conversion. In all non-diabetes patients, the AUC reached 0.88 (95% CI: 0.756–1.000) with an optimal cut-off value of 1.95 at which sensitivity was 90.0% (95% CI: 59.6–98.2%) and specificity was 81.3% (95% CI: 70.0–88.9%). The mean TB score was higher in patients with low bacterial loads (n = 31) than those with high bacterial loads (n = 91) at M0, M1, M2, and M6, and was higher in non-cavitary patients (n = 71) than those with cavitary lesions (n = 51) at M0, M1, and M2.</p></div><div><h3>Conclusion</h3><p>Xpert TB-score shows promising predictive value for culture conversion in non-diabetic TB patients. Sputum bacterial load and lung cavitation status have an influence on the value of TB score.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102521"},"PeriodicalIF":3.2,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simplified and efficient method for single-step, targeted gene deletion in mycobacteria 在分枝杆菌中进行单步定向基因缺失的简化高效方法

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-18 DOI: 10.1016/j.tube.2024.102520

Yahav Bracha, Daniel Barkan

引用次数: 0

Omadacycline drug susceptibility testing for non-tuberculous mycobacteria using oxyrase to overcome challenges with drug degradation 利用氧化酶克服药物降解难题，对非结核分枝杆菌进行奥美拉唑类药物药敏试验

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-13 DOI: 10.1016/j.tube.2024.102519

Gunavanthi D. Boorgula , Tawanda Gumbo , Sanjay Singh , Pamela J. McShane , Julie V. Philley , Shashikant Srivastava

{"title":"Omadacycline drug susceptibility testing for non-tuberculous mycobacteria using oxyrase to overcome challenges with drug degradation","authors":"Gunavanthi D. Boorgula , Tawanda Gumbo , Sanjay Singh , Pamela J. McShane , Julie V. Philley , Shashikant Srivastava","doi":"10.1016/j.tube.2024.102519","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102519","url":null,"abstract":"<div><h3>Background</h3><p>Drug susceptibility testing (DST) protocol of omadacycline against non-tuberculous mycobacteria has not yet been established. We developed a method to accurately determine MIC omadacycline MIC against <em>Mycobacterium abscessus</em> (Mab)<em>, Mycobacterium avium</em>-complex (MAC)<em>,</em> and <em>Mycobacterium kansasii</em> (Mkn).</p></div><div><h3>Methods</h3><p>First, we identified the oxyrase concentration not affecting Mab, MAC, and Mkn growth followed by omadacycline MIC experiments with and without oxyrase using reference and clinical strains.</p></div><div><h3>Results</h3><p>Oxyrase 0.5 % (v/v) stabilized omadacycline in the culture medium. The median omadacycline MIC was 1 mg/L for Mab and 8 mg/L for Mkn. For MAC, the median omadacycline MIC was 2 mg/L for <em>M</em>. <em>avium</em>, 256 mg/L for <em>M</em>. <em>intracellulare</em>, and 4 mg/L for <em>M</em>. <em>chimaera</em> (p < 0.0001). Wilcoxon matched-pairs signed rank test revealed statistically lower MICs with oxyrase for all MAC subspecies (p < 0.0001), all Mab subspecies (p < 0.0001), and Mkn (p = 0.0002). The decrease in MICs with oxyrase was 17/18 of Mab, 14/19 of Mkn, 8/8 of <em>M. avium</em>, 4/5 <em>M. chimera</em>, but only 11/18 of <em>M. intracellulare</em> (p < 0.013).</p></div><div><h3>Conclusion</h3><p>Use of 0.5 % oxyrase could be a potential solution to reliable and reproducible omadacycline MIC of Mab. However, oxyrase demonstrated a variable effect in reducing MICs against MAC and Mkn.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102519"},"PeriodicalIF":3.2,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140947029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bergenin potentiates BCG efficacy by enriching mycobacteria-specific adaptive memory responses via the Akt-Foxo-Stat4 axis Bergenin 通过 Akt-Foxo-Stat4 轴丰富分枝杆菌特异性适应性记忆反应，从而增强卡介苗的疗效

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-08 DOI: 10.1016/j.tube.2024.102517

Suparba Mukhopadhyay , Isha Pahuja , Ahmed Abdallah Okieh , Darshana Pandey , Vinod Yadav , Ashima Bhaskar , Ved Prakash Dwivedi

{"title":"Bergenin potentiates BCG efficacy by enriching mycobacteria-specific adaptive memory responses via the Akt-Foxo-Stat4 axis","authors":"Suparba Mukhopadhyay , Isha Pahuja , Ahmed Abdallah Okieh , Darshana Pandey , Vinod Yadav , Ashima Bhaskar , Ved Prakash Dwivedi","doi":"10.1016/j.tube.2024.102517","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102517","url":null,"abstract":"<div><p>The extensive inability of the BCG vaccine to produce long-term immune protection has not only accelerated the disease burden but also progressed towards the onset of drug resistance. In our previous study, we have reported the promising effects of Bergenin (Berg) in imparting significant protection as an adjunct immunomodulator against tuberculosis (TB). In congruence with our investigations, we delineated the impact of Berg on T cells, wherein it enhanced adaptive memory responses by modulating key transcription factors, STAT4 and Akt. We translated this finding into the vaccine model of TB and observed a notable reduction in the burden of <em>Mycobacterium tuberculosis</em> (<em>M.tb</em>) in BCG-Berg co-immunized mice as compared to BCG vaccination. Moreover, Berg, along with BCG, also aided in a heightened proinflammatory response milieu that corroborates the host protective immune response against TB. Furthermore, this response aligns with the escalated central and resident memory responses by modulating the Akt-Foxo-Stat4 axis, which plays a crucial role in enhancing the vaccine efficacy of BCG. These findings showcase the utilization of immunomodulator Berg as an immunoprophylactic agent to upgrade immunological memory, making it a more effective defender against TB.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102517"},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the cellulolytic activity of environmental mycobacteria 探索环境分枝杆菌的纤维素分解活性

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-08 DOI: 10.1016/j.tube.2024.102516

María Laura Mon , Nelson Romano , Pablo Daniel Farace , Claudia A. Tortone , Delia S. Oriani , Gianluca Picariello , Martín José Zumárraga , Andrea Karina Gioffré , Paola M. Talia

{"title":"Exploring the cellulolytic activity of environmental mycobacteria","authors":"María Laura Mon , Nelson Romano , Pablo Daniel Farace , Claudia A. Tortone , Delia S. Oriani , Gianluca Picariello , Martín José Zumárraga , Andrea Karina Gioffré , Paola M. Talia","doi":"10.1016/j.tube.2024.102516","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102516","url":null,"abstract":"<div><p>Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with <em>Mycolicibacterium septicum</em>. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that <em>M. septicum</em> encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102516"},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of AAICare®-TB sequence analysis tool for accurate diagnosis of drug-resistant tuberculosis: A comparative study with TB-Profiler and Mykrobe 评估 AAICare®-TB 序列分析工具对耐药结核病的准确诊断：与 TB-Profiler 和 Mykrobe 的比较研究

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-08 DOI: 10.1016/j.tube.2024.102515

Ritu Singhal , Smita Hingane , Manpreet Bhalla , Aniruddh Sharma , Sehnaz Ferdosh , Avlokita Tiwari , Praapti Jayaswal , Raj Narayan Yadav , Jyoti Arora , Ravindra Kumar Dewan , Sangeeta Sharma

{"title":"Evaluation of AAICare®-TB sequence analysis tool for accurate diagnosis of drug-resistant tuberculosis: A comparative study with TB-Profiler and Mykrobe","authors":"Ritu Singhal , Smita Hingane , Manpreet Bhalla , Aniruddh Sharma , Sehnaz Ferdosh , Avlokita Tiwari , Praapti Jayaswal , Raj Narayan Yadav , Jyoti Arora , Ravindra Kumar Dewan , Sangeeta Sharma","doi":"10.1016/j.tube.2024.102515","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102515","url":null,"abstract":"<div><p>A rapid and comprehensive drug susceptibility test is essential for eliminating drug resistant tuberculosis. Next generation sequencing (NGS) based susceptibility testing is being explored as a potential substitute for the conventional phenotypic and genotypic testing methods. However, the adoption of NGS based genotypic susceptibility testing depends on the availability of simple, accurate and efficient analysis tools. This preliminary study aimed to evaluate the performance of a <em>Mycobacterium tuberculosis</em> (Mtb) genome analysis pipeline, AAICare®-TB, for susceptibility prediction, in comparison to two widely used gDST prediction tools, TB-Profiler and Mykrobe. This study was performed in a National Reference Laboratory in India on presumptive drug-resistant tuberculosis (DR-TB) isolates. Whole genome sequences of the 120 cultured isolates were obtained through Illumina sequencing on a MiSeq platform. Raw sequences were simultaneously analysed using the three tools. Susceptibility prediction reports thus generated, were compared to estimate the total concordance and discordance. WHO mutation catalogue (1st edition, 2021) was used as the reference standard for categorizing the mutations. In this study, AAICare®-TB was able to predict drug resistance status for First Line (Streptomycin, Isoniazid, Rifampicin, Ethambutol and Pyrazinamide) and Second Line drugs (Fluoroquinolones, Second Line Injectables and Ethionamide) in 93 samples along with lineage and hetero-resistance as per the WHO guidelines.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102515"},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140918944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease 基于流式细胞仪的方法利用细胞因子产生的多样性区分结核分枝杆菌感染和疾病

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-08 DOI: 10.1016/j.tube.2024.102518

Karolina Dolezalova , Petra Hadlova , Marketa Ibrahimova , Jaroslav Golias , Lubos Baca , Emilia Kopecka , Mariia Sukholytka , Martina Koziar Vasakova

{"title":"Flow cytometry-based method using diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease","authors":"Karolina Dolezalova , Petra Hadlova , Marketa Ibrahimova , Jaroslav Golias , Lubos Baca , Emilia Kopecka , Mariia Sukholytka , Martina Koziar Vasakova","doi":"10.1016/j.tube.2024.102518","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102518","url":null,"abstract":"<div><p>Authors present a pilot study of the development of innovative flow cytometry-based assay with a potential for use in tuberculosis diagnostics. Currently available tests do not provide robust discrimination between latent tuberculosis infection (TBI) and tuberculosis disease (TB). The desired application is to distinguish between the two conditions by evaluating the production of a combination of three cytokines: IL-2 (interleukin-2), IFNɣ (interferon gamma) and TNFɑ (tumor necrosis factor alpha) in CD4<sup>+</sup> and CD8<sup>+</sup> T cells.</p><p>The study was conducted on 68 participants, divided into two arms according to age (paediatric and adults). Each arm was further split into three categories (non-infection (NI), TBI, TB) based on the immune reaction to <em>Mycobacterium tuberculosis (M.tb)</em> after a close contact with pulmonary TB. Each blood sample was stimulated with specific <em>M.tb</em> antigens present in QuantiFERON tubes (TB1 and TB2). We inferred TBI or TB based on the predominant cytokine response of the CD4<sup>+</sup> and/or CD8<sup>+</sup> T cells.</p><p>Significant differences were detected between the NI, TBI and the TB groups in TB1 in the CD4<sup>+</sup>TNFɑ<sup>+</sup>parameter in children. Along with IL-2, TNFɑ seems to be the most promising diagnostic marker in both CD4<sup>+</sup>and CD8<sup>+</sup> T cells. However, more detailed analyses on larger cohorts are needed to confirm the observed tendencies.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102518"},"PeriodicalIF":3.2,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000441/pdfft?md5=5289809f9fd61d36e7f0baf054089f2d&pid=1-s2.0-S1472979224000441-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuated tuberculin skin test responses associated with Mycobacterium intracellulare sputum colonization in an adolescent TB prevalence survey in Western Kenya 肯尼亚西部一项青少年结核病患病率调查显示，结核菌素皮试反应减弱与结核分枝杆菌痰内定植有关

IF 3.2 3区医学

Tuberculosis Pub Date : 2024-05-05 DOI: 10.1016/j.tube.2024.102514

Lilian N. Njagi , Grace Kaguthi , Jared O. Mecha , Thomas R. Hawn , Videlis Nduba

{"title":"Attenuated tuberculin skin test responses associated with Mycobacterium intracellulare sputum colonization in an adolescent TB prevalence survey in Western Kenya","authors":"Lilian N. Njagi , Grace Kaguthi , Jared O. Mecha , Thomas R. Hawn , Videlis Nduba","doi":"10.1016/j.tube.2024.102514","DOIUrl":"https://doi.org/10.1016/j.tube.2024.102514","url":null,"abstract":"<div><h3>Introduction</h3><p>Exposure to Non-tuberculous Mycobacteria (NTM) varies regionally and may partly explain the disparate outcomes of BCG vaccination and tuberculosis (TB) susceptibility.</p></div><div><h3>Methods</h3><p>We examined NTM sputum colonization, associations with clinical characteristics, and tuberculin skin test (TST) responses in an adolescent TB prevalence survey.</p></div><div><h3>Results</h3><p>Among 5004 adolescents screened, 2281 (45.5 %) were evaluated further. TB and NTM prevalence rates were 0.3 % and 8.0 %, respectively. Among 418 NTM isolates, 103 were unidentifiable, and 315 (75 %) comprised 15 species, the most frequent being <em>M. intracellulare</em> (MAC) (108, 26 %), <em>M. scrofulaceum</em> (96, 23 %) and <em>M. fortuitum</em> (51, 12 %). “NTM colonized” adolescents had less frequent chronic cough and night sweats (adjusted odds ratio [aOR] 0.62, 95 % confidence interval [CI] 0.44-0.87and aOR 0.61, CI 0.42–0.89 respectively), and lower TST induration (median 11 mm (interquartile range [IQR] 0–16) vs 13 mm (IQR 6–17; <em>p</em> = 0.006)) when compared to “NTM not colonized” participants. MAC, but not <em>M. scrofulaceum</em> or <em>M. fortuitum</em>, was associated with decreased TST induration (median 7.5 mm (IQR 0–15) vs 13 mm (IQR 6–17) among “MAC colonized” vs “not colonized”, <em>p</em> = 0.001).</p></div><div><h3>Conclusion</h3><p>We observed high NTM prevalence rates with species-specific associations with TST induration, consistent with a model of species-dependent heterologous immunity among mycobacteria.</p></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"147 ","pages":"Article 102514"},"PeriodicalIF":3.2,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1472979224000404/pdfft?md5=598b622cc212bbdae86a171b82b459cf&pid=1-s2.0-S1472979224000404-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0