Tuberculosis最新文献

{"title":"Biomarkers for diagnosing pulmonary tuberculosis in adolescents: Peripheral blood monocyte myotubularin-related protein 4 and oncostatin M genes","authors":"Yuxia Sha , Haoquan Zhou , Bingda Yan , Xianzong Da , Meilin Shao , Ye Li , Shenggang Ding","doi":"10.1016/j.tube.2025.102676","DOIUrl":"10.1016/j.tube.2025.102676","url":null,"abstract":"<div><h3>Background/objectives</h3><div>Although research has increasingly been focused on pulmonary tuberculosis (PTB) in adolescents, its diagnosis remains complex and challenging. Thus, the use of host transcription markers in the diagnosis of adolescent PTB was evaluated in this study.</div></div><div><h3>Methods</h3><div>The study cohort comprised 40 adolescents (aged 14–18 years) with PTB who had received their first PTB diagnosis at Anhui Provincial Chest Hospital, China, between January and December 2023 (case group) and 32 healthy adolescents who had undergone physical examinations at The First Affiliated Hospital of the University of Science and Technology of China during the same period (control group). Peripheral blood samples were collected from both groups, and isolated monocytes were subjected to transcriptome sequencing and bioinformatic analysis. The relevant genes were confirmed through quantitative polymerase chain reaction. The diagnostic value of these genes in adolescent PTB patients was analysed using receiver operating characteristic curves. Furthermore, an in vitro model was constructed by infecting THP-1 cells with the <em>Mycobacterium tuberculosis</em> strain H37Ra to assess whether the in vitro expression levels of the identified genes were consistent with those of the genes in the peripheral blood monocytes of adolescents with PTB. The specific mechanisms were explored using methods such as lentiviral infection, flow cytometry, immunofluorescence, Western blotting, and qRT‒PCR.</div></div><div><h3>Results</h3><div>The results revealed that the expression level of the <em>myotubularin-related protein 4</em> gene (<em>MTMR4</em>) was lower in the case group than in the control group (<em>P</em> < 0.0001; area under the curve: 0.946; 95 % confidence interval: 0.893–0.999; cut-off value: 0.844; sensitivity: 0.875; specificity: 0.969). The expression level of the <em>oncostatin M</em> gene (<em>OSM</em>) was greater in the case group than in the control group (<em>P</em> = 0.014; area under the curve: 0.962; 95 % confidence interval: 0.914–1.000; cut-off value: 0.919; sensitivity: 0.950; specificity: 0.969). However, no significant between-group difference was detected in the expression level of the complement <em>component 1q subcomponent A</em> gene. The results from the in vitro experiment indicated that the expression levels of <em>MTMR4</em> and <em>OSM</em> were consistent between the H37Ra-infected cells and the case group samples. Bioinformatics analysis revealed that cytokine–cytokine receptor interactions, JAK/STAT signalling and PI3K/cell survival-related pathways play key roles in the pathogenesis of adolescent PTB. Additionally, we found that H37Ra infection affected tuberculosis progression via the OSM/PI3K/GPX4 pathway.</div></div><div><h3>Conclusions</h3><div>Peripheral blood monocyte <em>OSM</em> and <em>MTMR4</em> may serve as biomarkers of adolescent PTB. We speculate that targeting the OSM signallin","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102676"},"PeriodicalIF":2.9,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144809517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence of infectious diseases in children with Mycobacterium tuberculosis in Japan, 2007–2022","authors":"Yuko Hamaguchi ,&nbsp;Takayuki Yamaguchi ,&nbsp;Takashi Yoshiyama","doi":"10.1016/j.tube.2025.102674","DOIUrl":"10.1016/j.tube.2025.102674","url":null,"abstract":"<div><div>The objective of this study was to understand the descriptive epidemiology of childhood tuberculosis (TB) in Japan under the 2013 Bacillus Calmette–Guérin (BCG) immunization program modification. The median percentage of annual vaccination coverage for infants aged &lt;13 months was 97.0 % during follow-up (2007–2022). The age at which most infants received their vaccinations was 3 months before 2013 and 5 months after 2013. During follow-up, the number of childhood TB notifications and annual incidence declined by approximately 60 % and 40 %, respectively. A multivariate-adjusted model analysis was performed by birth year to determine the association between childhood TB and the 2013 BCG immunization program modification. However, childhood TB was associated with age and BCG vaccination history but not with the 2013 BCG immunization program modification. This study represents a valuable resource for future research, elucidating the efficacy of BCG as well as the impact of BCG policies.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102674"},"PeriodicalIF":2.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144769104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population pharmacokinetics of levofloxacin in drug-susceptible and drug-resistant tuberculosis patients: Optimal dose suggestion based on renal function","authors":"Yong-Soon Cho ,&nbsp;Rannissa Puspita Jayanti ,&nbsp;Hyun-Kyung Lee ,&nbsp;Hyo-Jung Kim ,&nbsp;Tae Won Jang ,&nbsp;Yun Seok Kim ,&nbsp;Yousang Ko ,&nbsp;Jinsoo Min ,&nbsp;Heayon Lee ,&nbsp;Soedarsono Soedarsono ,&nbsp;Hyeon-Jeong Seong ,&nbsp;Young-Kyung Choi ,&nbsp;Ho-Sook Kim ,&nbsp;Dong Hyun Kim ,&nbsp;Jae-Gook Shin ,&nbsp;cPMTb","doi":"10.1016/j.tube.2025.102675","DOIUrl":"10.1016/j.tube.2025.102675","url":null,"abstract":"<div><h3>Background</h3><div>Levofloxacin (LFX) has gained attention as an effective drug to reduce treatment duration in tuberculosis (TB). We aimed to evaluate factors related to interindividual variability (IIV) and describe the pharmacokinetics (PK) of LFX in both DS- and DR-TB, as well as explore the optimal dose for TB treatment.</div></div><div><h3>Methods</h3><div>We included demographics, clinical information, and LFX concentrations from multinational hospitals. All data were utilized for model establishment. The population PK model was built using nonlinear mixed-effects method. Dose simulation was carried out thereafter using Monte Carlo simulation.</div></div><div><h3>Results</h3><div>A one-compartment model with allometric scaling described LFX PK adequately. PK parameters were similar between DS- and DR-TB. eGFR significantly affected CL/F, which decreased by 22 % and 48 % in mild and moderate-severe renal impairment, respectively (normal CL/F: 6.6 L/h). Considering LFX's AUC/MIC target of 146 and epidemiological cut-off value of MIC 0.5 μg/mL, doses of 1000 mg, 1250 mg, and 1500 mg may achieve 90 % probability of target attainment in patients with normal renal function weighing &lt;40 kg, 40–70 kg, and &gt;70 kg, respectively.</div></div><div><h3>Conclusion</h3><div>Renal impairment reduced LFX clearance. Doses equal to or greater than 1000 mg may improve AUC/MIC target attainment but require cautious use considering safety and clinical efficacy.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102675"},"PeriodicalIF":2.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144724164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of non-tuberculous mycobacteria in slaughtered cattle from Chennai, India","authors":"Harini Ramanujam ,&nbsp;Manohar Nesakumar ,&nbsp;Kannan Thiruvengadam ,&nbsp;Rajaraman Kannan ,&nbsp;Sivaraman Palanisamy ,&nbsp;Sivakumar Shanmugam ,&nbsp;Kannan Palaniyandi","doi":"10.1016/j.tube.2025.102673","DOIUrl":"10.1016/j.tube.2025.102673","url":null,"abstract":"<div><div>Non-tuberculous mycobacteria (NTM) are emerging pathogens in human and veterinary medicine, with a globally increasing incidence. In India, sporadic studies have identified an upward trend in NTM infections, but accurate prevalence estimates are lacking due to the absence of nationwide surveillance. Non-tuberculous mycobacteria have been reported in clinically healthy cattle and wildlife globally, complicating tuberculosis (TB) diagnostics and surveillance. This study aimed to characterize NTM species isolated from tissue samples of slaughtered cattle in Chennai using culture and targeted <em>hsp65</em> gene sequencing. A total of 118 presumed NTM samples from 115 animals were processed, and 49 isolates were confirmed as NTMs by PCR. Sequencing identified 18 different species, with <em>Mycobacterium intracellulare</em> (9/49) being the most frequent, followed by <em>Mycobacterium</em> sp. <em>strain 79_MI18_10584</em> (6/49) and <em>Mycobacterium elephantis</em> (6/49). Several identified species, including <em>M. intracellulare, M. fortuitum</em> (5/49)<em>, M. kansasii</em> (4/49)<em>, and M. avium</em>, have caused infections in humans as well. NTMs in cattle lymph nodes without visible lesions suggest their asymptomatic persistence, albeit there being a possibility of transient colonization. Non-tuberculous mycobacteria complicate bovine tuberculosis (bTB) diagnostics by inducing cross-reactive immune responses and forming granulomatous lesions resembling those caused by <em>Mycobacterium tuberculosis</em> complex (MTBC). This study highlights the presence and diversity of NTMs in Indian cattle and emphasizes the need for better surveillance, improved molecular characterization, and better understanding of their epidemiological and immunological roles in both veterinary and public health contexts.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102673"},"PeriodicalIF":2.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144722939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of monocyte-associated genes MSRB2, CLEC4D, and ASGR2 as potential biomarkers for tuberculosis via machine learning and mendelian randomization","authors":"Zihan Cai ,&nbsp;Yuyang Zhou ,&nbsp;Chun Bi ,&nbsp;Shoupeng Ding","doi":"10.1016/j.tube.2025.102671","DOIUrl":"10.1016/j.tube.2025.102671","url":null,"abstract":"<div><h3>Objective</h3><div>This study explores the link between immune cells and tuberculosis (TB) pathogenesis and progression, proposing diagnostic strategies based on immune microenvironment changes.</div></div><div><h3>Methods</h3><div>The CIBERSORT algorithm assessed immune cell infiltration in TB tissues, validated by routine blood tests. Differential expression analysis and WGCNA identified key genes and modules. GO and KEGG analyses elucidated biological functions. Machine learning pinpointed diagnostic biomarkers and built a predictive model. Further validation included GSVA, single-cell data, Mendelian randomization, and RT-qPCR.</div></div><div><h3>Results</h3><div>Analysis of the immune microenvironment in TB patients and healthy controls revealed monocytes as the predominant immune cell type. A total of 90 overlapping genes were identified through differential expression analysis and WGCNA. A diagnostic model incorporating MSRB2, CLEC4D, and ASGR2 was constructed using three distinct machine learning algorithms and logistic regression. Single-cell data analysis demonstrated that these three genes were predominantly expressed in mononuclear cells of TB patients. MR analysis further established a causal relationship between CLEC4D and an elevated risk of TB.</div></div><div><h3>Conclusion</h3><div>We established a monocyte-based diagnostic model demonstrating robust predictive accuracy. MSRB2, CLEC4D, and ASGR2 represent promising therapeutic targets for TB immunotherapy, providing potential breakthroughs in diagnostic precision and treatment efficacy.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102671"},"PeriodicalIF":2.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNAs in exhaled breath Condensate: Novel non-invasive biomarkers for tuberculosis diagnosis","authors":"Tak Jaya ,&nbsp;Pattnaik Bijay ,&nbsp;Rai Divyanjali ,&nbsp;Bhatraju Naveen ,&nbsp;P.P. Adwika ,&nbsp;Bangaru Sunil ,&nbsp;Sunita Yadav ,&nbsp;Sachin kumar ,&nbsp;Seetu Kumari ,&nbsp;Umashankar Verma ,&nbsp;Geetika Yadav ,&nbsp;R.S. Dhaliwal ,&nbsp;Saurabh Mittal ,&nbsp;Pawan Tiwari ,&nbsp;Vijay Hadda ,&nbsp;Karan Madan ,&nbsp;Anurag Agrawal ,&nbsp;Randeep Guleria ,&nbsp;Tapasya Srivastava ,&nbsp;Anant Mohan","doi":"10.1016/j.tube.2025.102670","DOIUrl":"10.1016/j.tube.2025.102670","url":null,"abstract":"<div><h3>Background</h3><div>Tuberculosis remains a significant global health issue. Sputum smear microscopy has low sensitivity, making it difficult to diagnose. Analysis of miRNA from EBC (exhaled breath condensate) offers a potential non-invasive diagnostic method that could improve sensitivity and specificity for TB detection, including extrapulmonary TB (EPTB).</div></div><div><h3>Method</h3><div>EBC was collected from 65 treatment naïve TB patients and 65 healthy controls. miRNA profiling was done on an exploratory set (n = 40) using the qRT-PCR-based miRNome profiler kit, and shortlisted miRNAs were validated in a separate set (n = 25) using qRT-PCR. ROC curves were used to evaluate diagnostic performance.</div></div><div><h3>Result</h3><div>In this study, we identified eight differentially expressed miRNAs in TB vs healthy subjects (seven upregulated, one downregulated). Comparing PTB with EPTB, five miRNAs were upregulated and 68 miRNAs were downregulated in PTB. Between PTB and healthy controls, six miRNAs were upregulated and 16 miRNAs were downregulated in PTB, while in EPTB, 132 miRNAs were upregulated compared with controls. Validation confirmed upregulation of miR-454, miR-139, and miR-143 in tuberculosis.</div></div><div><h3>Conclusion</h3><div>The findings of our study indicate that the expression of miR-143, miR-454, and miR-139 in exhaled breath condensate has strong potential as a non-invasive biomarker for TB diagnosis.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102670"},"PeriodicalIF":2.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient cell model for assessing inflammatory responsive genes in Mycobacterium tuberculosis and SARS-CoV-2 co-infection","authors":"Thays Maria Costa de Lucena ,&nbsp;Débora Elienai de Oliveira Miranda ,&nbsp;Juliana Vieira de Barros Arcoverde ,&nbsp;Mariana Souza Bezerra Cavalcanti ,&nbsp;Willyenne Marilia Dantas ,&nbsp;Lindomar José Pena ,&nbsp;Virginia Maria Barros de Lorena ,&nbsp;Michelle Christiane da Silva Rabello ,&nbsp;Jaqueline de Azevedo Silva","doi":"10.1016/j.tube.2025.102672","DOIUrl":"10.1016/j.tube.2025.102672","url":null,"abstract":"<div><div><em>Mycobacterium tuberculosis</em> (<em>Mtb</em>) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) may induce immunopathology with extensive lung damage in hosts. To elucidate the dynamics of co-infection <em>Mtb</em> and SARS-CoV-2 and its impact on inflammatory mediators’ expression, we conducted a study to evaluate A549, lung epithelial cells, as a potential model for hosting both pathogens simultaneously. Cell infection initiated with <em>Mtb</em> H37Rv and following a 24-h incubation period, the cells were then infected with SARS-CoV-2. After a 72 h incubation period, a precision test was conducted for both pathogens, and total RNA was extracted for subsequent analysis of gene expression by RT-qPCR of the target genes: <em>IFN-γ</em>, <em>TNF-α</em>, <em>IL-6</em>, and <em>IL-1β</em>. Additionally, the levels of IL-1β, IL-2, IL-4, IL-6, IL-10, IFN-γ, and TNF-α in the culture supernatants were measured. A549 cells are a stable and reliable cellular model for co-infection between <em>Mycobacterium tuberculosis</em> and SARS-CoV-2. Co-infection with both pathogens led to downregulation of IFN-γ, TNF-α, and IL-10, and upregulation of IL-6 and IL-1β compared to uninfected cells. A549 cells function as a cellular model for co-infection and seems a good model for elucidating host inflammatory responses in the initial site of infection.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102672"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring novel salivary host biomarkers for immunological diagnosis of tuberculosis: A preliminary biomarker discovery study","authors":"Pavithra Selvan ,&nbsp;Nalini Jayanthi Nagesh ,&nbsp;Leela Kakithakara Vajravelu","doi":"10.1016/j.tube.2025.102669","DOIUrl":"10.1016/j.tube.2025.102669","url":null,"abstract":"<div><div>Tuberculosis is a serious public health concern on a global scale, which emphasises the critical need for quick and precise diagnostic and treatment response monitoring techniques. In this study, Luminex multiplex immunoassay was used to detect the concentrations of 37 host biomarkers in saliva samples from 46 patients newly diagnosed with active pulmonary tuberculosis (PTB) and 46 patients with other respiratory diseases (ORD). Multiple logistic regression and the area under the receiver operator characteristics curve (AUC) were used to evaluate the diagnostic accuracy of biomarkers, which showed significant differences between the 2 groups. This study reported that Fractalkine exhibited the highest diagnostic accuracy and excellent discriminatory power, with statistically significant results (p ≤ 0.05), an AUC of 0.91, 89.1 % sensitivity and 76.1 % specificity, highlighting its strong potential to distinguish PTB cases from ORD cases. Additionally, our study found that the median levels of IL-17A, IL-23, and VEGF were statistically significant (p ≤ 0.05). General discriminant analysis further identified Fractalkine, VEGF, GM-CSF, IL-23, and IL-1α as the top five most effective biomarkers for combinations. The backward elimination approach demonstrated the potential usefulness of a four-marker combination (Fractalkine + GM-CSF + IL-23 + IL-1α) as a confirmatory diagnostic tool by achieving the greatest overall diagnostic accuracy with an AUC of 0.94 and 91.3 % specificity. Thus, combining multiple markers with high discriminating power may improve diagnostic performance and subsequently provide a more accurate, non-invasive saliva-based PTB diagnostic tool.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102669"},"PeriodicalIF":2.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144563629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hereditary and antimicrobial factor shaping extracellular bacteria dynamics in an in-host mathematical model of tuberculosis for disease control","authors":"Morufu Oyedunsi Olayiwola ,&nbsp;Ezekiel Abiodun Oluwafemi","doi":"10.1016/j.tube.2025.102668","DOIUrl":"10.1016/j.tube.2025.102668","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a global health challenge, necessitating deeper insights into the dynamics of extracellular bacterial populations within infected hosts. This study presents an in-host mathematical model that incorporates hereditary and antimicrobial factors influencing TB progression. The biological feasibility of the model is established by analyzing the boundedness of solutions within a realistic parameter space. The equilibrium states, including the disease-free and endemic equilibria, are examined, revealing conditions under which the system remains locally asymptotically stable. Sensitivity analysis is conducted to determine the key parameters driving infection dynamics, providing insights into potential control strategies. Notably, the model exhibits a backward bifurcation, indicating the possibility of multiple stable states and suggesting that reducing the basic reproduction number R₀ below unity may not be sufficient for disease eradication. These findings highlight the importance of targeted interventions to effectively control extracellular bacterial populations and mitigate TB infection.</div></div>","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102668"},"PeriodicalIF":2.8,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144556722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPRi-mediated repression of efflux pumps reveals Rv1258c as a key contributor to pyrazinamide resistance in Mycobacterium tuberculosis","authors":"Carlos Alonso Flores B. ,&nbsp;Kiara Aricoche-Del Campo ,&nbsp;Robert H. Gilman ,&nbsp;Mirko Zimic ,&nbsp;Patricia Sheen","doi":"10.1016/j.tube.2025.102665","DOIUrl":"10.1016/j.tube.2025.102665","url":null,"abstract":"","PeriodicalId":23383,"journal":{"name":"Tuberculosis","volume":"154 ","pages":"Article 102665"},"PeriodicalIF":2.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}