Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献_第3页

Protective effects of α-lipoic acid and L-carnitine in liver ischemia/reperfusion injury. α-硫辛酸和左旋肉碱对肝脏缺血再灌注损伤的保护作用。

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.02525

G. S. Ozgun, Eray Özgün, U. Başaran, Ş. Altaner, N. Sut, S. Eskiocak

{"title":"Protective effects of α-lipoic acid and L-carnitine in liver ischemia/reperfusion injury.","authors":"G. S. Ozgun, Eray Özgün, U. Başaran, Ş. Altaner, N. Sut, S. Eskiocak","doi":"10.5505/TJB.2014.02525","DOIUrl":"https://doi.org/10.5505/TJB.2014.02525","url":null,"abstract":"Objective: Oxidative stress plays an important role in the pathogenesis of liver ischemia/ reperfusion injury. Thus, antioxidant treatment can be protective against to liver ischemia/reperfusion injury. The aim of this study to investigate the effects of α-lipoic acid and L-carnitine on liver total oxidant status, lipid peroxidation, protein oxidation, neutrophil infiltration and hepatic necrosis in liver ischemia/reperfusion model. Methods: Wistar albino male rats were divided into four groups randomly: Sham (n=7), ischemia/reperfusion (n=7), α-lipoic acid (n=8) and L-carnitine (n=8). α-Lipoic acid (100 mg/kg) and L-carnitine (100 mg/kg) were given intraperitoneally to α-lipoic acid group 15 minutes before and to L-carnitine group 30 minutes before ischemia/reperfusion protocol, respectively. To induce hepatic ischemia/reperfusion injury, ischemia (60 minutes) and reperfusion (30 minutes) were applied to all groups except sham group. Total oxidant status, malondialdehyde, advanced oxidation protein products and myeloperoxidase levels were measured in ischemic lobes of liver tissues. Hepatic necrosis was scored microscopically. Results: There was no significant change in myeloperoxidase levels as an indicator of neutrophil infiltration after reperfusion procedure. Both L-carnitine and α-lipoic acid caused a significant decrease in hepatic necrosis. While L-carnitine prevents an increase in total oxidant status, lipid peroxidation and protein oxidation, α-lipoic acid prevents only an increase in lipid peroxidation of the liver in hepatic ischemia/reperfusion injury. Conclusion: As a result; we can report that L-carnitine and α-lipoic acid have protective effects against to hepatic ischemia/reperfusion injury.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"30 1","pages":"169-175"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78706873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Relationship Between Insulin Resistance and Leptin, Interleukin-6, hs-CRP and Fibrinogen in Obese and Non-Obese Individuals 肥胖者与非肥胖者胰岛素抵抗与瘦素、白细胞介素-6、hs-CRP及纤维蛋白原的关系

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.20981

A. Koçak, R. Kutlu, S. Çivi, I. Kilinç

引用次数: 0

Evaluation of the serum visfatin and adiponectin levels in patients with type 2 diabetes mellitus 2型糖尿病患者血清内脂素和脂联素水平的评价

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.87487

M. Kara, S. Uslu, N. Kebapçı, E. Ozcelik, C. Bal

{"title":"Evaluation of the serum visfatin and adiponectin levels in patients with type 2 diabetes mellitus","authors":"M. Kara, S. Uslu, N. Kebapçı, E. Ozcelik, C. Bal","doi":"10.5505/TJB.2014.87487","DOIUrl":"https://doi.org/10.5505/TJB.2014.87487","url":null,"abstract":"Objective: The purpose of this study was to assess the levels of serum adiponectin and visfa- tin in patients with Type 2 Diabetes Mellitus and to investigate their potential roles in insulin resistance and obesity in T2DM. Methods: The study was carried out in 45 patients with T2DM and 20 sex and age matched healthy control subjects (n=20). According to the body mass index (BMI) 45 patients were divided into two subgroups; one group was nonobese diabetic patients with 18.50 < BMI < 24.99kg/m 2 (n=20) and the other group was type 2 diabetic obese diabetic patients with BMI ≥30kg/m 2 (n=25). Serum adiponectin and visfatin levels were determined by using ELISA (Enzyme-Linked Immuno-Sorbent Assay) method. The insulin resistance index was as- sessed by homeostasis model assessment for insulin resistance (HOMA-IR). Results: Serum adiponectin levels in obese and non-obese diabetic subjects was low when compared to the control group ( p<0.001 and p<0.01; respectively). Conversely adiponectin, visfatin levels compared with control was higher in obese diabetic (p<0.001). When adi- ponectin was negatively correlated with duration of diabetes, body mass index, HOMA-IR, HbA1c, and glucose, visfatin was positively correlated with HOMA-IR and body mass index. Conclusion: Diabetic patients compared with healthy control group decreased serum adipo- nectin and increased serum visfatin levels may be useful in the elucidation of the connection between obesity - insulin resistance.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"28 1","pages":"181-187"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81237388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Importance of HbA1c and Fructosamine As A Marker Of Glycemic Control and Evaluation Of Some Biochemical Parameters During Pregnancy 妊娠期糖化血红蛋白和果糖胺作为血糖控制指标的重要性及一些生化指标的评价

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.15238

R. Şeker, E. Ozdemir, G. Çağlar, S. Demirtaş

{"title":"Importance of HbA1c and Fructosamine As A Marker Of Glycemic Control and Evaluation Of Some Biochemical Parameters During Pregnancy","authors":"R. Şeker, E. Ozdemir, G. Çağlar, S. Demirtaş","doi":"10.5505/TJB.2014.15238","DOIUrl":"https://doi.org/10.5505/TJB.2014.15238","url":null,"abstract":"Objective: Controlling and monitoring the diabetes during pregnancy, because of the malformation, macrosomia and other complications is especially important. Our study is designed to investigate the reliability of the two most commonly used parameter is the hemoglobin A1c (HbA1c) and fructosamine during pregnancy. Also among our study group, serum total protein, albumin, vitamin B12, folic acid, glucose, insulin, ferritin and hemoglobin data were evaluated. Methods: The study groups are pregnant women (n=252; age mean: 27,761± 5/year) and control group (n=28; age mean: 28,61± 5,38/year) who are no additional health problems. Diabetic pregnant women number is 124 (consisted of 10 first trimester, 62 second trimester and 52 third trimester) and nondiabetic pregnant women number is 138 (consisted of 24 first trimester, 32 second trimester and 82 third trimester). All measurement data are obtained from hospital information system. Results: No statistically significant difference in any of the groups for HbA1c. It’s clearly observed that all the significant differences found for fructosamine occur depending on the level of albumin and fructosamine levels decreased progressively during the three trimesters. In our diabetic pregnant women group; although statistically make a difference; mean blood glucose values were below our expectations. Conclusion: Our study is support that fructosamine and HbA1c are unavailable for diagnosis and monitoring the gestational diabetes. However the lack of difference between the groups for levels of fructosamine and HbA1c; and blood glucose levels below our expectations in diabetic pregnant women group can be connected good diabetes control.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"60 1","pages":"336-343"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80869555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of agilent 1100 (chromsystems) and tosoh hlc-723 g8 hplc systems in thalassemia screening agilent 1100 (chromsystems)与tosoh hlc- 7238 - hplc系统在地中海贫血筛查中的比较

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/tjb.2015.80774

H. Ellidağ

引用次数: 2

Determination of acidic dissociation constants of glutamine and isoleucine in water using ab initio methods 用从头算法测定谷氨酰胺和异亮氨酸在水中的酸性解离常数

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.04578

F. Koohyar

{"title":"Determination of acidic dissociation constants of glutamine and isoleucine in water using ab initio methods","authors":"F. Koohyar","doi":"10.5505/TJB.2014.04578","DOIUrl":"https://doi.org/10.5505/TJB.2014.04578","url":null,"abstract":"Objective: In this study, pKa values of glutamine and isoleucine were determined in aqueous solution by ab initio and DFT methods. To explain the obtained values of pKa, the molecular conformations and solute-solvent interactions of the anions, cations, and neutrals molecules of glutamine and isoleucine were investigated. The experimental determination of these values, apart from been laborious, is a challenge because of the low water solubility of these compounds. Methods: We have evaluated different models to determination of pKa, using the density functional theory (DFT) method at the B3LYP level of the theory. Results: this study shows the several ionization reactions and equilibriums in protic solvent, which possess a high hydrogen-band-donor capability. These reactions and equilibriums constitute the indispensable theoretical basis for calculation of glutamine and isoleucine acidity constants. Tomasi’s method was used to analyze the formation of intermolecular hydrogen bonds between the existent species and water molecules. In this way, it is proposed that in alkaline aqueous solutions the cation, anion, and neutral species of glutamine and isoleucine are solvated with one, two, three, and four molecules of water, respectively. In this study, there is comparable agreement between the experimental and calculated pKa values for the acid-base reactions proposed. Conclusion: In this paper, The calculations performed at the B3LYP/6-31+G(d) levels of theory using Tomasi’s method allowed us to prove that cations, neutral molecules, and anions form IHBs with some molecules of water. It is shown that, theoretically calculated pKa values are in good agreement with the existing experimental pKa values, which are determined from potentiometric titration and UV–visible spectrophotometric measurements.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"535 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85696798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Site-Directed Mutagenesis of Cephalosporin C Acylase and Enzymatic Conversion of Cephalosporin C to 7-Aminocephalosporanic Acid 头孢菌素C酰化酶的定点突变及头孢菌素C转化为7-氨基头孢菌素酸的研究

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.48569

Yuanyuan Ren, Yulin Lei, Yushan Zhu

{"title":"Site-Directed Mutagenesis of Cephalosporin C Acylase and Enzymatic Conversion of Cephalosporin C to 7-Aminocephalosporanic Acid","authors":"Yuanyuan Ren, Yulin Lei, Yushan Zhu","doi":"10.5505/TJB.2014.48569","DOIUrl":"https://doi.org/10.5505/TJB.2014.48569","url":null,"abstract":"Objective: A cephalosporin C acylase catalyzes hydrolysis of cephalosporin C to 7-aminocephalosporanic acid directly. This work was considered helpful for the further study of the cephalosporin C acylase and also useful for the strain improvement. Methods: Its mutant (G139αS/F58βN/I75βT/I176βV/S471βC) named A12 was cloned into pET28a (+) vector and expressed in E.coli BL21 (DE3). The three dimentional structure of A12 was constructed by the homology modeling and its’ catalytic sites was analyzed by the DOCK software. Results: The mutant A12 was expressed in E.coli BL21 (DE3) with the molecular weight 87kDa containing two subunits of 58kDa α-subunit and 25kDa β-subunit. The activity of A12 was 291 U/L which was lower than that of AcyII (322 U/L) because of the low expression level. The specific activity of A12 was 6.011 U/mg which was higher than that of the AcyII (2.868 U/ mg). Catalytic analysis suggested that A12 had the improved catalytic efficiency (kcat/Km) to convert cephalosporin C to 7-ACA at the beginning of the reaction. These results combined with the model analysis indicated that Phe58β、Ile75β and Ile176β were involved in the catalysis from CPC to 7-ACA. Conclusion: In this work, the gene of cephalosporin C acylase AcyII was synthesized, mutated and expressed successfully in the E.coli BL21 (DE3). The specific activity and the catalytic efficiency of A12 increased 2-fold and 3-fold respectively. Compared with the study of cephalosporin C acylase in N176, this work was considered helpful for the further study of the catalytic mechanism of cephalosporin C acylase and also useful for the strain improvement for the cephalosporin C acylase production.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"14 1","pages":"51-56"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78130936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide 不同绝色图塔菌株对阿维菌素的抗性分析(鳞翅目:蠓科

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.09327

M. Konuş

{"title":"Analysing resistance of different Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) strains to abamectin insecticide","authors":"M. Konuş","doi":"10.5505/TJB.2014.09327","DOIUrl":"https://doi.org/10.5505/TJB.2014.09327","url":null,"abstract":"Objective: Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), tomato leafminer, is an oligophagous insect. Larvae of T. absoluta can destroy especially tomato plants which lead to important yield loss in this economically valuable crop. Chemical control through insecticides has been a main method of controlling it in farming areas all over the world. However, continues application of certain registered insecticide such as abamectin might lead to resistance development in T. absoluta. The aim of this study was to monitor resistance status of abamectin insecticide and analyse resistance mechanisms of this insecticide in T. absoluta field populations from three districts of Turkey by using bioassay and biochemical methods. Methods: Bioassays and Biochemical assays. Results: Bioassay results showed that while Adana and Antalya strain of T. absoluta showed low resistance (3.03and 2.3-fold) to abamectin insecticide, Ankara strain of T. absoluta was not resistant to abamectin (1.31-fold). Biochemical analysis displayed that CYP450-PNOD activities showed 2.55 and 1.95-fold increase compared to susceptible population in Adana and Antalya field populations, respectively. Furthermore, GST-CDNB activities showed statistically significant (p<0.05) 1.3-fold increase only in Adana population. Although EST-α-NA activities showed 3.41-fold increase only in Ankara field population, this field population did not display a significant resistancy to abamectin. Conclusion: Consequently, cytochrome P450 monooxygenase enzymes seemed to have a major role in abamectin resistance development in field populations of T. absoluta from Turkey. In addition, GSTs possibly have supportive role such as reducing oxidative stress that developed during metabolism of abamectin in resistant field populations of T. absoluta.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"43 1","pages":"291-297"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91270818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Usage potential of acetylcholinesterase as a bioscavenger in organophasphate poisoning 乙酰胆碱酯酶作为有机磷酸酯中毒生物清除剂的应用潜力

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.00922

Tuba Tuylu Kuçukkilinç

引用次数: 1

Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2 新分离的嗜气芽孢杆菌KGJ2产木聚糖酶的优化

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.92300

D. Gowdhaman, Ganesan Jeyalakshmi, Karuppiah Sugumaran, Nagarajan Sai Subramanian, R. Santhosh, Venkatachalam Ponnusami

引用次数: 6