新分离的嗜气芽孢杆菌KGJ2产木聚糖酶的优化

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI:10.5505/TJB.2014.92300

D. Gowdhaman, Ganesan Jeyalakshmi, Karuppiah Sugumaran, Nagarajan Sai Subramanian, R. Santhosh, Venkatachalam Ponnusami

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引用次数: 6

摘要

目的:本研究的目的是从造纸厂废水中分离出一种潜在的新型芽孢杆菌菌株，用于生产工业上重要的木聚糖酶。材料与方法:利用木聚糖琼脂培养基的清除率，从造纸厂废水中分离出一株产木聚糖酶活性强的微生物。通过16S rRNA分析和生化鉴定鉴定菌株。对分离菌株产生的木聚糖酶进行了部分纯化，并对其活性和稳定性进行了表征。结果:该嗜气芽孢杆菌KGJ2产木聚糖酶为嗜热菌，在70℃时表现出较高的活性和稳定性。木聚糖酶在pH为4.0时活性达到峰值，具有良好的酸稳定性。结果表明，桦木木聚糖和牛肉提取物分别是最合适的碳源和氮源。结论:结果证实嗜气芽孢杆菌KGJ2产生独特的酸-

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Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2

Objective: The objective of the present study was to isolate a potential and novel Bacillus strain from paper mill effluent for the production of an industrially important xylanase. Material and Methods: A potent xylanase producing microorganism was isolated from paper mill effluent based on zone of clearance on xylan agar medium. The strain was identified based on 16S rRNA analysis and biochemical characterization. Xylanase produced by the isolated strain was partially purified and characterized for its activity and stability. Results: The xylanase produced by this Bacillus aerophilus KGJ2 was thermophilic, shows higher activity and stability at 70°C. Xylanase had activity peak at pH 4.0 and was very acid stable. Birchwood xylan and beef extract were identified as best suited carbon source and nitrogen source, respectively. Conclusion: The results confirm that Bacillus aerophilus KGJ2 produced a unique acido-

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来源期刊

Turkish Journal of Biochemistry-turk Biyokimya Dergisi 生物-生化与分子生物学

CiteScore

1.20

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.