Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献

Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF 165 ) in Arabidopsis thaliana 重组血管内皮生长因子165 (VEGF 165)在拟南芥中的表达

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2019-06-26 DOI: 10.1515/TJB-2017-0368

Qiang Weidong, F. Xue, Li Yixin, L. Xinxin, J. Kun, Sun Xiaoyu, C. Xi, Li Haiyan, Du Linna, Yang Jing

{"title":"Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF 165 ) in Arabidopsis thaliana","authors":"Qiang Weidong, F. Xue, Li Yixin, L. Xinxin, J. Kun, Sun Xiaoyu, C. Xi, Li Haiyan, Du Linna, Yang Jing","doi":"10.1515/TJB-2017-0368","DOIUrl":"https://doi.org/10.1515/TJB-2017-0368","url":null,"abstract":"Objective: Targeting the protein of interest to a particular tissue to achieve high-level expression is an important strategy to increase expression efficiency. The use of the plant seed oil body as a bioreactor can not only increase the amount of target protein, but also reduce the cost of downstream processing. Methods: VEGF165 was expressed in Arabidopsis thaliana seeds via oilbody fusion technology. The pKO-VEGF165 vector was construted and transformed into A. thaliana seeds. T3 transgenic seeds was detected by SDS-PAGE and western blot methods. The cell activity was tested by MTT methods. Result: The phaseolin promoter was used to drive seedspecific expression of the VEGF165 gene in transgenic A. thaliana. The coding region of VEGF165 was fused to the Arabidopsis oleosin sequence to target the protein to the oil bodies in the seeds of transgenic plants. The T-DNA region of recombinant plasmid pKO-VEGF165 was shifted to A. thaliana seeds via the floral-dip method. Protein was analyzed by electrophoresis and protein hybridization analyses. Finally, MTT assays showed that the oleosinVEGF165 fusion protein played a part in the proliferation of HUVEC cells in vitro. Conclusion: Oleosin-VEGF165 was successfully expressed and it had stimulated HUVEC cell proliferation activity.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89073120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Dietary Chicken Grill Oil and Sunflower Seed Oil on Blood and Liver Oxidant/Antioxidant Status and Liver Function Tests in Laying Japanese Quails (Coturnix coturnix japonica) 鸡扒油和葵花籽油对产蛋日本鹌鹑血液和肝脏氧化/抗氧化状态及肝功能的影响

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.81894

T. Buyukoglu

引用次数: 1

PKC stimulation increases expression of cholinesterases in R28 cell line PKC刺激增加R28细胞系胆碱酯酶的表达

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.38981

E. Bodur, P. Layer

引用次数: 1

Biosorption Studies For Removal of Cu (II) ions Onto Saccharum Bengalense an Efficient and Eco-Friendly Biosorbent 高效环保生物吸附剂班加兰糖对Cu (II)离子的生物吸附研究

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.25483

M. I. Din

{"title":"Biosorption Studies For Removal of Cu (II) ions Onto Saccharum Bengalense an Efficient and Eco-Friendly Biosorbent","authors":"M. I. Din","doi":"10.5505/TJB.2014.25483","DOIUrl":"https://doi.org/10.5505/TJB.2014.25483","url":null,"abstract":"Objective: In this research work, a low-cost biosorbent derived from the pulp of Saccharum bengalense (SB) was used as an adsorbent material for the removal of Cu (II) ions from aqueous solution. Methods: A batch adsorption method was experimented for biosorptive removal of copper ions from the aqueous solution. Results: Nearly 90% removal of copper is possible at initial pH 6.0, under the batch test conditions. Langmuir, Freundlich and Dubinin–Radushkevich (D–R) adsorption isotherms have been applied to describe the biosorption of Cu (II) by SB. It was found that biosorption of Cu (II) by SB followed Langmuir and Freundlich model. The monolayer adsorption capacity of SB as obtained from Langmuir isotherm at 40 0C was found to be 10.42 mg/g at 323K. The Cu (II) adsorption data was analyzed by applying pseudo-first-order and pseudo-second-order kinetic models. The intraparticle diffusion model was applied to investigate the rate-determining step. It was found that the biosorption of Cu (II) ions on SB followed pseudo second-order rate kinetics. Thermodynamic parameters such as free energy change (ΔG°), enthalpy change (ΔH°) and entropy change (ΔS°) have been calculated respectively revealed the spontaneous, feasible and endothermic nature of adsorption process. Conclusion: The results of the present investigation suggested that biosorbent SB can be used as an economically feasible and environmentally friendly biosorbent for the removal of Cu (II) from aqueous solutions.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78543427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Toxicopathological evaluation of Picralima nitida seed aqueous extract in Wistar rats 小檗籽水提物对Wistar大鼠的毒性病理评价

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.83997

T. Sunmonu, O. B. Oloyede, T. Owolarafe, M. Yakubu, O. O. Dosumu

{"title":"Toxicopathological evaluation of Picralima nitida seed aqueous extract in Wistar rats","authors":"T. Sunmonu, O. B. Oloyede, T. Owolarafe, M. Yakubu, O. O. Dosumu","doi":"10.5505/TJB.2014.83997","DOIUrl":"https://doi.org/10.5505/TJB.2014.83997","url":null,"abstract":"Objective: Picralima nitida is a widely used medicinal plant in West Africa for treating malaria, diarrhea and inflammation. The objective of this study is to evaluate the toxicological effect of aqueous seed extract of the plant in Wistar rats. Methods: Twenty-four apparently healthy animals were randomized into 4 groups comprising 6 rats each and orally administered with aqueous extract of P. nitida seeds at doses of 100, 200 and 400 mg/kg body weight with distilled water as control for 14 days. Specific liver and kidney function indices were assayed alongside haematological and histopathological analyses to monitor toxicity according to standard methods. Results: Phytochemical screening revealed the presence of alkaloids, glycosides, saponins, steroids and tannins. The extract had no significant effect on all kidney function indices assayed but caused a significant reduction (P < 0.05) in the activities of liver enzymes accompanied by significant decrease in liver to body weight ratio, serum total protein and globulin concentrations. No significant alteration was observed in the serum levels of albumin and conjugated bilirubin whereas the extract brought about significant increase (P < 0.05) in serum total bilirubin concentration. Haematological analysis revealed no significant effect on erythrocyte indices in contrast to white blood cell count and its differentials which were significantly elevated (P < 0.05) following extract administration. Histopathological studies further showed no distortion of cell structures in the studied organs. Conclusion: The available evidences in this study suggest that aqueous extract of P. nitida seeds exhibits mild and selective toxicity with liver as the target organ. Therefore, the herb may not be completely 'safe' as an oral remedy; and long term administration should be avoided.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89915249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Platelet function disorders 血小板功能障碍

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/tjb.2015.80958

Z. Dikmen

引用次数: 0

The effect of L-carnitine on nitric oxide metabolism in streptozotocin-induced diabetic rats 左旋肉碱对链脲佐菌素诱导的糖尿病大鼠一氧化氮代谢的影响

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.04934

G. S. Ozgun

{"title":"The effect of L-carnitine on nitric oxide metabolism in streptozotocin-induced diabetic rats","authors":"G. S. Ozgun","doi":"10.5505/TJB.2014.04934","DOIUrl":"https://doi.org/10.5505/TJB.2014.04934","url":null,"abstract":"Objective: The aim of this study is to investigate the effect of L-carnitine on plasma and liver nitric oxide metabolism in streptozotocin-induced diabetic rats. Methods: Sprague Dawley female rats were divided randomly into following groups: control, Lcarnitine, diabetes and diabetes+L-carnitine. Diabetes and diabetes+L-carnitine groups were intraperitonally injected with a single dose of streptozotocin (40 mg/kg) prepared in the citrate buffer (pH 4.5). Other groups were injected with only citrate buffer. 72 hours after the streptozotocin injection, Lcarnitine (500 mg/kg/day) was given intraperitoneally to L-carnitine and diabetes+L-carnitine groups for 15 days. Physiological saline was given intraperitoneally to the other groups for 15 days. Blood sugar (at 72 hours and the end of experiment), liver nitric oxide and inducible nitric oxide synthase, plasma nitric oxide and nitrotyrosine levels were measured. Results: Blood glucose levels in diabetic groups were higher compared with other groups. Percentage change of blood glucose in diabetes+L-carnitine group was lower compared with other groups. Also diabetes+L-carnitine group’s plasma nitric oxide levels were higher than control group. Plasma nitrotyrosine levels of L-carnitine injected groups were lower than diabetes group. There was no significant difference between the levels of liver inducible nitric oxide synthase and nitric oxide in groups. Conclusion: As a result, our study showed that plasma and liver nitric oxide and liver inducible nitric oxide synthase levels aren’t changed significantly but plasma nitrotyrosine levels are increased at the end of 15th day of experimental diabetes. On the other hand, our results also showed that L-carnitine causes an increase in plasma nitric oxide levels and a decrease in plasma nitrotyrosine levels whereas it has no effect on liver nitric oxide and inducible nitric oxide synthase levels.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84179503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Real-Time PCR Analysis of Pyrethroid Resistance in Helicoverpa armigera from Turkey 土耳其棉铃虫拟除虫菊酯抗性的实时PCR分析

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.50490

M. Konuş, S. U. Karaağaç, M. Işcan

引用次数: 1

Platelet function tests 血小板功能试验

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2015.17894

Z. Dikmen

引用次数: 0

Evaluation of the serum visfatin and adiponectin levels in patients with type 2 diabetes mellitus 2型糖尿病患者血清内脂素和脂联素水平的评价

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.87487

M. Kara, S. Uslu, N. Kebapçı, E. Ozcelik, C. Bal

{"title":"Evaluation of the serum visfatin and adiponectin levels in patients with type 2 diabetes mellitus","authors":"M. Kara, S. Uslu, N. Kebapçı, E. Ozcelik, C. Bal","doi":"10.5505/TJB.2014.87487","DOIUrl":"https://doi.org/10.5505/TJB.2014.87487","url":null,"abstract":"Objective: The purpose of this study was to assess the levels of serum adiponectin and visfa- tin in patients with Type 2 Diabetes Mellitus and to investigate their potential roles in insulin resistance and obesity in T2DM. Methods: The study was carried out in 45 patients with T2DM and 20 sex and age matched healthy control subjects (n=20). According to the body mass index (BMI) 45 patients were divided into two subgroups; one group was nonobese diabetic patients with 18.50 < BMI < 24.99kg/m 2 (n=20) and the other group was type 2 diabetic obese diabetic patients with BMI ≥30kg/m 2 (n=25). Serum adiponectin and visfatin levels were determined by using ELISA (Enzyme-Linked Immuno-Sorbent Assay) method. The insulin resistance index was as- sessed by homeostasis model assessment for insulin resistance (HOMA-IR). Results: Serum adiponectin levels in obese and non-obese diabetic subjects was low when compared to the control group ( p<0.001 and p<0.01; respectively). Conversely adiponectin, visfatin levels compared with control was higher in obese diabetic (p<0.001). When adi- ponectin was negatively correlated with duration of diabetes, body mass index, HOMA-IR, HbA1c, and glucose, visfatin was positively correlated with HOMA-IR and body mass index. Conclusion: Diabetic patients compared with healthy control group decreased serum adipo- nectin and increased serum visfatin levels may be useful in the elucidation of the connection between obesity - insulin resistance.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81237388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7