Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献

{"title":"The Relationship Between Insulin Resistance and Leptin, Interleukin-6, hs-CRP and Fibrinogen in Obese and Non-Obese Individuals","authors":"A. Koçak, R. Kutlu, S. Çivi, I. Kilinç","doi":"10.5505/TJB.2014.20981","DOIUrl":"https://doi.org/10.5505/TJB.2014.20981","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"102 1","pages":"373-382"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79381085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of α-lipoic acid and L-carnitine in liver ischemia/reperfusion injury.","authors":"G. S. Ozgun, Eray Özgün, U. Başaran, Ş. Altaner, N. Sut, S. Eskiocak","doi":"10.5505/TJB.2014.02525","DOIUrl":"https://doi.org/10.5505/TJB.2014.02525","url":null,"abstract":"Objective: Oxidative stress plays an important role in the pathogenesis of liver ischemia/ reperfusion injury. Thus, antioxidant treatment can be protective against to liver ischemia/reperfusion injury. The aim of this study to investigate the effects of α-lipoic acid and L-carnitine on liver total oxidant status, lipid peroxidation, protein oxidation, neutrophil infiltration and hepatic necrosis in liver ischemia/reperfusion model. Methods: Wistar albino male rats were divided into four groups randomly: Sham (n=7), ischemia/reperfusion (n=7), α-lipoic acid (n=8) and L-carnitine (n=8). α-Lipoic acid (100 mg/kg) and L-carnitine (100 mg/kg) were given intraperitoneally to α-lipoic acid group 15 minutes before and to L-carnitine group 30 minutes before ischemia/reperfusion protocol, respectively. To induce hepatic ischemia/reperfusion injury, ischemia (60 minutes) and reperfusion (30 minutes) were applied to all groups except sham group. Total oxidant status, malondialdehyde, advanced oxidation protein products and myeloperoxidase levels were measured in ischemic lobes of liver tissues. Hepatic necrosis was scored microscopically. Results: There was no significant change in myeloperoxidase levels as an indicator of neutrophil infiltration after reperfusion procedure. Both L-carnitine and α-lipoic acid caused a significant decrease in hepatic necrosis. While L-carnitine prevents an increase in total oxidant status, lipid peroxidation and protein oxidation, α-lipoic acid prevents only an increase in lipid peroxidation of the liver in hepatic ischemia/reperfusion injury. Conclusion: As a result; we can report that L-carnitine and α-lipoic acid have protective effects against to hepatic ischemia/reperfusion injury.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"30 1","pages":"169-175"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78706873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The first observation of two alpha globin mutations in Turkey: Hb Stanleyville II and a homozygous 5nt deletion","authors":"Figen Guzelgul","doi":"10.5505/TJB.2014.31967","DOIUrl":"https://doi.org/10.5505/TJB.2014.31967","url":null,"abstract":"Objective: In this study, we aimed to contribute prenatal diagnosis in the Çukurova region having high prevalence of hemoglobinopathies by implementing DNA sequencing analysis for the mutations undetectable by conventional methods. Methods: Hematological parameters of two families applied to Cukurova University Prenatal Diagnosis Center were analyzed. Hb variants were screened by cellulose acetate electrophoresis and by HPLC. DNA samples of cases were isolated using automatic DNA extraction machine. Microarray, RFLP, ARMS and Gap-PCR molecular methodologies carried out for determinations of hemoglobin mutations. Unidentified mutations were resolved by means of DNA Sequence Analysis. Results: We identified a previously unreported homozygous 5 nt deletional mutation [α2 IVS-1 134.-138. nt (TGAGG)] in Turkey by DNA Sequence Analysis. When we investigated twelve cases of this family by Gap-PCR and DNA Sequence Analysis it is observed that seven cases were heterozygous for 5nt deletion, a case found to be heterozygous 20.5 kb deletional mutation and four cases were found to be having no mutation. In the other family applied for prenatal diagnosis was observed to having Hb Stanleyville II [α2 78 (EF 7) Asn→ Lys (AAC→AAA)] mutation which is also a novel mutaion in Turkey. Mutations of eleven members of this family were determined by ARMS, RFLP, Gap-PCR and DNA Sequence Analysis, resulting in two cases heterozygous Hb Stanleyville II, five cases heterozygous 3.7 kb deletion, a case with heterozygous IVS-1-110 (G→C) mutation on β globin gene and three cases do not have any mutation. Conclusion: In this study, two types of mutations observed for the first time in Turkey have been identified.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"56 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76472441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERUM LIPIDS AND Lp-PLA2 ACTIVITY IN MULTIPLE SCLEROSIS","authors":"H. Doğan","doi":"10.5505/TJB.2014.27147","DOIUrl":"https://doi.org/10.5505/TJB.2014.27147","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"33 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77573207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the median levels of double and triple prenatal screening parameters in Adıyaman region","authors":"P. G. Atak, A. Arpacı, Gulsah Seydal","doi":"10.5505/TJB.2014.20591","DOIUrl":"https://doi.org/10.5505/TJB.2014.20591","url":null,"abstract":"Objective: The purpose of this study is to determine the median values of the triple and double test screening parameters in Adiyaman region. Methods: Between January 2010-December 2011, triple tests were performed to 5909, double tests were performed to 5310 singleton pregnant women in Adiyaman KadinDogum Hastanesi Biochemistry Laboratory. Serum alpha-fetoprotein (AFP), unconjugated estriol (uE3), beta-human chorionic gonadotrophin (β-hCG) for triple test and serum β-hCG, pregnancy associated plasma protein-A (PAPP-A) for double test were investigated from the patients archives retrospectively. The new calculated medians of the Adiyaman region and the existing computer program’s medians were evaluated statistically. Results: The first trimester screening parameters’ median values of beta-human chorionic gonadotrophin and pregnancy associated plasma protein-A were significantly lower than the program’s median values (p<0.05). The medians of AFP were significantly lower than the program’s medians in 15-19 gestation weeks (p<0.05). The medians of uE3 were significantly higher than the programmes’ medians (p<0.05) except 20. gestation week. β-hCG medians of 16,19 and 20’th gestational weeks were statistically different than the programme’s medians (p<0.05), the difference between 15, 17 and 18’th gestational weeks’ values were statistically unnecessary. The calculated first trimester screening parameters’ median values of β-hCG and PAPPA were significantly lower than the program’s median values in all gestational weeks (p<0.05). For triple test the medians of AFP were lower than the programme’s medians in all weeks and this was statistically significant except 20th gestational week (p<0.05). The medians of uE3 were significantly higher than the programs’ medians (p<0.05) except 20th gestation week. β-hCG medians of 16,19 and 20th gestational weeks were statistically different than the programm’s medians (p<0.05), the difference between 15., 17. and 18th gestational weeks’ values were not statistically significant. Conclusion: The screening test performances will be affected by using the regional median values as in former studies.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"84 1","pages":"231-237"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90301410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-processing of agro-industrial waste orange peel for induced production of pectinase by Trichoderma viridi; its purification and characterization","authors":"M. Irshad, Z. Anwar, Z. Mahmood, T. Aqil, S. Mehmmod, H. Nawaz","doi":"10.5505/TJB.2014.55707","DOIUrl":"https://doi.org/10.5505/TJB.2014.55707","url":null,"abstract":"Objective: Agro-industrial residues are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially important enzymes. For industrial use, pectinases can be produced from several agricultural pectin-containing wastes like orange peel. The selection of cheap source benefits the production of industrial important enzymes in term to costs-effective production. Methods: The indigenously produced pectinase was purified to homogeneity level using a combination of ammonium sulphate precipitation and Sephadex G-100 gel filtration chromatography. The molecular weight and properties of the purified enzyme were studied following the standard procedures. Results: Under optimized solid state fermentation conditions Trichoderma viridi exhibited superior enzymatic production. On optimization, the culture showed the maximum enzyme yield (325 U/mL) at 30 °C in an orange peel medium having a pH of 5.5 and a substrate concentration of 4% on the 4th day of fermentation of orange peel based medium that was additionally supplemented with glucose and ammonium chloride as an inexpensive carbon and nitrogen supplements in a ratio of 20:1, respectively. A purification fold of 5.59 with specific activity and percent recovery of 97.2 U/mg and 12.96% was achieved respectively. The molecular weight of purified pectinase from T. viridi was 30 kDa evidenced by PAGE analysis. After 6 h incubation the pectinase activity profile showed that the purified enzyme was optimally active and stable at a pH of 5 and at 60°C. Conclusions: The present study concluded that the indigenous strain T. viridi showed incredible potential for pectinase synthesis. The maximum production of pectinase in the presence of a cheaper substrate at low concentrations makes the enzyme useful in industrial sectors, especially for the textile and juice industries.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"66 1","pages":"9-18"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89334156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of high fat diets with and without N-Acetylcysteine supplementation on the lipin-1 levels of serum and various tissues in rats","authors":"B. Kural, A. Alver, S. Canpolat, C. Kahraman, D. Altay, Hanife Kara, Buket Akcan","doi":"10.5505/TJB.2014.78941","DOIUrl":"https://doi.org/10.5505/TJB.2014.78941","url":null,"abstract":"Objective: Obesity is a public health problem, but the struggle against obesity continues to increase every day. Lipin-1 is an adipokine that modulates lipid metabolism and glucose homeostasis. The aims of the current study were to compare the levels of lipin-1 in serum and tissues (liver, lung, heart, pancreas and kidney) in rat fed with low fat diet (LFD), high fat diet (HFD) and N-acetylcysteine (NAC) supplemented HFD, and to determine the relationships of the lipin-1 levels in serum and tissues with the levels of serum glucose and lipid parameters. Methods: 18 male Sprague-Dawley rats were divided into three groups: LFD group (n=6), HFD group (n=6) and the NAC-supplemented HFD (HFD+NAC) group (n=6). The feeding period of the diets was 85 days. The lipin-1 levels in serum and tissues were determined by using Rat ELISA kit. Results: In comparison to serum lipin-1 levels in HFD group were significantly lower, but heart lipin-1 levels were significantly higher (P<0.017). On the other hand, it was determined that serum and tissue lipin-1 levels were not influenced by NAC supplementation. In addition, no other significant difference in lipin-1 levels of the other tissues among the groups was found. Conclusion: This study showed that HDF affected serum and heart lipin-1 levels, while NAC supplementation did not affect on the lipin-1 levels of serum and of the tissues.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"20 1","pages":"19-24"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82792725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The reference values of ischemia modified albumin levels in healthy people admitted to a university hospital","authors":"C. Duman, C. Bakar, Ertan Eşsi̇zoğlu, Funda Kirtay Tutunculer, Elif Demircan","doi":"10.5505/TJB.2014.32932","DOIUrl":"https://doi.org/10.5505/TJB.2014.32932","url":null,"abstract":"Objectives: The Ischemia Modified Albumin (IMA) test is a myocardial ischemia marker which may have potential use for the diagnosis of Acute Coronary Syndrome. The aim of this study was determine reference values of IMA test on the healthy people over the age of 15 who's admitted to Canakkale Onsekiz Mart University. Methods: This study was performed on 582 people over 15 years old and healthy in appearan- ce who had presented at the Canakkale Onsekiz Mart University Faculty of Medicine Rese- arch and Application Hospital. The year 2011 population of Turkey was taken as the reference in the distribution of the study group according to age group and gender. A survey which queried the demographic features and health problems was administered to the participants of the study and 9 cc venous blood was drawn. IMA levels were measured by using the spect- rophotometric method defined by Bar-Or. The mean, standard deviation, median, minimum, maximum, and 2.5 % - 97.5 % interval were used in order to detect the IMA reference values. Results: A total of 274 males (47.1 %) and 308 women (52.9 %) were studied. The mean IMA value of the examined group was 0.339 ± 0.093, and the median was 0.343 (Min-Max: 0.053- 0.631), while the 2.5 % - 97.5 % percentil values were 0.145 - 0.530. Conclusion: Studies that indicate the reference levels of the IMA for the diagnosis of ische- mic conditions are insufficient. Our study is expected to contribute to closing this gap.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"1 1","pages":"132-139"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82829567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the serum visfatin and adiponectin levels in patients with type 2 diabetes mellitus","authors":"M. Kara, S. Uslu, N. Kebapçı, E. Ozcelik, C. Bal","doi":"10.5505/TJB.2014.87487","DOIUrl":"https://doi.org/10.5505/TJB.2014.87487","url":null,"abstract":"Objective: The purpose of this study was to assess the levels of serum adiponectin and visfa- tin in patients with Type 2 Diabetes Mellitus and to investigate their potential roles in insulin resistance and obesity in T2DM. Methods: The study was carried out in 45 patients with T2DM and 20 sex and age matched healthy control subjects (n=20). According to the body mass index (BMI) 45 patients were divided into two subgroups; one group was nonobese diabetic patients with 18.50 < BMI < 24.99kg/m 2 (n=20) and the other group was type 2 diabetic obese diabetic patients with BMI ≥30kg/m 2 (n=25). Serum adiponectin and visfatin levels were determined by using ELISA (Enzyme-Linked Immuno-Sorbent Assay) method. The insulin resistance index was as- sessed by homeostasis model assessment for insulin resistance (HOMA-IR). Results: Serum adiponectin levels in obese and non-obese diabetic subjects was low when compared to the control group ( p<0.001 and p<0.01; respectively). Conversely adiponectin, visfatin levels compared with control was higher in obese diabetic (p<0.001). When adi- ponectin was negatively correlated with duration of diabetes, body mass index, HOMA-IR, HbA1c, and glucose, visfatin was positively correlated with HOMA-IR and body mass index. Conclusion: Diabetic patients compared with healthy control group decreased serum adipo- nectin and increased serum visfatin levels may be useful in the elucidation of the connection between obesity - insulin resistance.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"28 1","pages":"181-187"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81237388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C-Reactive Protein vs. High - Sensitivity C - Reactive Protein: What is the Difference?","authors":"D. Yücel","doi":"10.5505/TJB.2014.92408","DOIUrl":"https://doi.org/10.5505/TJB.2014.92408","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"65 1","pages":"43-44"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72846072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}