Optimization of the xylanase production with the newly isolated Bacillus aerophilus KGJ2

Abstract

Objective: The objective of the present study was to isolate a potential and novel Bacillus strain from paper mill effluent for the production of an industrially important xylanase. Material and Methods: A potent xylanase producing microorganism was isolated from paper mill effluent based on zone of clearance on xylan agar medium. The strain was identified based on 16S rRNA analysis and biochemical characterization. Xylanase produced by the isolated strain was partially purified and characterized for its activity and stability. Results: The xylanase produced by this Bacillus aerophilus KGJ2 was thermophilic, shows higher activity and stability at 70°C. Xylanase had activity peak at pH 4.0 and was very acid stable. Birchwood xylan and beef extract were identified as best suited carbon source and nitrogen source, respectively. Conclusion: The results confirm that Bacillus aerophilus KGJ2 produced a unique acido-