Transfusion最新文献_第3页

Misoprostol administration mimicking a febrile transfusion reaction.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-05 DOI: 10.1111/trf.18150

Nalan Yurtsever, Edward L Snyder

{"title":"Misoprostol administration mimicking a febrile transfusion reaction.","authors":"Nalan Yurtsever, Edward L Snyder","doi":"10.1111/trf.18150","DOIUrl":"https://doi.org/10.1111/trf.18150","url":null,"abstract":"Background: Misoprostol, a synthetic prostaglandin E1 analogue, is widely used in obstetrics for its uterotonic properties. It is known to cause thermogenic side effects, a fact well-recognized in obstetrics but less familiar in transfusion medicine.Study design and methods: Data were collected through chart review, including temperature recordings, serologic findings, and blood culture results.Results: This case report describes a postpartum temperature spike to 39.2°C in a G1P0 female who received a red cell transfusion shortly after administration of misoprostol. Serologic workup for transfusion reaction showed no abnormalities, and blood cultures of both the patient and donor were negative for growth. Febrile nonhemolytic transfusion reaction (FNHTR) remained in the differential, however, the fever was attributed to misoprostol's thermogenic effect rather than a transfusion reaction.Discussion: Increased awareness of misoprostol's thermogenic effects in transfusion medicine may improve differential diagnosis, reduce unnecessary testing, and enhance patient satisfaction by avoiding unwarranted concerns regarding transfusion reactions.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143190533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality of whole blood stored in room temperature for up to 5 days.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-05 DOI: 10.1111/trf.18133

Sanna Susila, Tuukka Helin, Lotta Joutsi-Korhonen, Jouni Lauronen, Minna Ilmakunnas

{"title":"Quality of whole blood stored in room temperature for up to 5 days.","authors":"Sanna Susila, Tuukka Helin, Lotta Joutsi-Korhonen, Jouni Lauronen, Minna Ilmakunnas","doi":"10.1111/trf.18133","DOIUrl":"https://doi.org/10.1111/trf.18133","url":null,"abstract":"Background: Non-refrigerated whole blood can be used for bleeding emergencies when cold-stored whole blood is unavailable. Storage time in room temperature is usually limited to 24 h although there is little evidence supporting this practice. We studied the quality of whole blood stored in room temperature for 5 days to investigate the effects of prolonged storage time.Study design and methods: Non-leukoreduced whole blood in CPDA-1 from 10 group O or A RhD positive male donors was stored in +22°C for 120 h. Samples were taken daily to assess blood cultures, blood count and metabolic parameters. Platelet function and blood coagulation were evaluated with multiple electrode aggregometry, viscoelastic tests (sonorheometry and rotational thromboelastometry), thrombin generation assay and measurements of individual clotting factors.Results: Blood cell counts remained stable during storage. Metabolic changes were similar to those previously reported in cold-stored blood products. Most coagulation factor levels, including FVIII, decreased during storage but remained within physiological range. Thrombin generation remained mostly intact during storage. In viscoelastic tests, clotting times prolonged, but clot strength remained stable. Platelet function in multiple electrode aggregometry impaired along with storage. No bacterial growth was detected in any sample.Conclusion: Whole blood stored in room temperature for 5 days seems bacteriologically safe and retains most of its metabolic and hemostatic function. These results suggest that whole blood stored in room temperature may be usable for longer than the currently recommended 24 h.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of whole blood versus red blood cells and plasma to correct trauma-induced coagulopathy ex vivo.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-05 DOI: 10.1111/trf.18143

Andrea Rossetto, Paul Vulliamy, Sian Huish, Rebecca Cardigan, Laura Green, Ross Davenport

{"title":"Comparison of whole blood versus red blood cells and plasma to correct trauma-induced coagulopathy ex vivo.","authors":"Andrea Rossetto, Paul Vulliamy, Sian Huish, Rebecca Cardigan, Laura Green, Ross Davenport","doi":"10.1111/trf.18143","DOIUrl":"https://doi.org/10.1111/trf.18143","url":null,"abstract":"Background: Early resuscitation is based on platelet-poor components such as red blood cells and plasma (RBC + P), contributing to platelet dilution and worsening of trauma-induced coagulopathy (TIC). We aimed to compare the ability of cold-stored whole blood (WB) versus RBC + P as a single component to correct TIC.Study design and methods: Blood samples were collected on admission from trauma patients who required activation of the major hemorrhage protocol at a single UK major trauma center in 2021/2022. Samples were spiked ex vivo with volumes equivalent to two, four, or eight units of WB or RBC + P stored for a maximum of 2 weeks. Thromboelastometry, platelet counting, and multiple electrode aggregometry (MEA) were performed.Results: Samples from 20 adult trauma patients were analyzed. Median age was 32 years (27-42), 89% were male, 70% had platelet dysfunction (tissue factor-activated ROTEM [EXTEM]-tissue factor-activated ROTEM with cytochalasin D [FIBTEM] clot amplitude at 5 min [A5] ≤ 30 mm), 65% were coagulopathic (EXTEM A5 ≤ 40 mm), and 42% died. EXTEM-FIBTEM A5 was higher following spiking with WB than RBC + P (33 mm, 26-33, vs. 27 mm, 24-30, p < .001). WB-spiking corrected platelet dysfunction in 2 patient samples out of 20, whereas RBC + P increased the frequency of platelet dysfunction (1/20 sample) and TIC (4/20 samples). RBC + P was associated with a dose-dependent deterioration in rotational thromboelastometry (ROTEM) clot strength and dynamics, platelet count, and aggregation in response to multiple agonists compared with WB-spiking, which maintained or partially corrected these abnormalities.Conclusion: Compared with RBC + P, WB better preserves ex vivo platelet-related ROTEM parameters, platelet count, and aggregation, but does not fully correct these common derangements of TIC.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperviscosity syndrome in Waldenström Macroglobulinemia.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-02 DOI: 10.1111/trf.18152

José Antonio Sánchez Salas, María Sánchez-Villalobos, Marta Palazón de la Torre, Maite Valentina Serrano-Pérez, Estela Ruiz Ruiz, Valentín Cabanas-Perianes

引用次数: 0

Eliminating leukocyte reduction for whole blood: Is it premature to consider this paradigm-changing practice? 消除全血白细胞减少：考虑这种改变范式的做法是否为时过早？

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 Epub Date: 2024-12-22 DOI: 10.1111/trf.18113

Brian D Adkins, Garrett S Booth, Ross M Fasano, Eric A Gehrie, Mark L Gestring, Debra Masel, Phuong-Lan T Nguyen, Majed A Refaai, Jeremy W Jacobs, Sheharyar Raza, Michael A Vella, Christopher A Tormey, Neil Blumberg

引用次数: 0

Further thoughts on a lower fibrinogen threshold prior to therapeutic plasma exchange.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 DOI: 10.1111/trf.18111

Kamran Urgun, Jesse Qiao, Gagan Mathur, Minh-Ha Tran

引用次数: 0

In reply.

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 DOI: 10.1111/trf.18112

Amena Usmani, Ravi Sarode

引用次数: 0

RHCE genotyping using next generation sequencing: Allele specific reference sequences. 使用下一代测序的RHCE基因分型：等位基因特异性参考序列。

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 Epub Date: 2024-12-22 DOI: 10.1111/trf.18106

Wajnat A Tounsi, Amr J Halawani, Kelly A Sillence, Michele Kiernan, Neil D Avent, Tracey E Madgett

{"title":"RHCE genotyping using next generation sequencing: Allele specific reference sequences.","authors":"Wajnat A Tounsi, Amr J Halawani, Kelly A Sillence, Michele Kiernan, Neil D Avent, Tracey E Madgett","doi":"10.1111/trf.18106","DOIUrl":"10.1111/trf.18106","url":null,"abstract":"Background: The Rh blood group system (ISBT004) is encoded by two homologous genes, RHD and RHCE. Polymorphism in these two genes gives rise to 56 antigens, which are highly immunogenic and clinically significant. This study extended previous work on the establishment of RHD allele specific reference sequences using next generation sequencing (NGS) with the Ion Personal Genome Machine (Ion PGM) to sequence the complete RHCE gene.Study design and methods: Genomic DNA (gDNA) samples (n = 87) from blood donors of different serologically predicted genotypes including R1R1 (DCe/DCe), R2R2 (DcE/DcE), R1R2 (DCe/DcE), R2RZ (DcE/DCE), R1r (DCe/dce), R2r (DcE/dce), R0r (Dce/dce), rr (dce/dce), r'r (dCe/dce), and r″r (dcE/dce) were used in this study. The RHCE gene was amplified through overlapping long range-polymerase chain reaction (LR-PCR) amplicons and then sequenced with the Ion PGM. Data were analyzed against the human genome reference sequence build hg38 and variants were called.Results: Referen variant allel VS. In addition to the RHCE reference alleles, different exonic single nucleotide variants (SNVs) were detected that encode known RHCE variant alleles including RHCE*Ce.09, RHCE*ceAR, and RHCE*ceVS.03. Numerous intronic SNVs were detected and compared from samples with different Rh genotypes, to determine their link to a specific Rh haplotype. Based on the exonic and intronic changes detected in different RHCE alleles, three RHCE reference sequences were established and submitted to Genbank (one for the RHCE*Ce allele, one for the RHCE*cE allele, and one for the RHCE*ce allele).Conclusion: Intronic SNVs may represent a novel alternative diagnostic approach to investigate known and novel variants of the RH genes and the prediction of Rh haplotype.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"363-374"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The REALITY of MINT: Caution before changing transfusion practice in myocardial infarction based on recent clinical trials. 薄荷的现实：根据最近的临床试验，在改变心肌梗死的输血做法之前要谨慎。

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 Epub Date: 2025-01-19 DOI: 10.1111/trf.18109

Aryeh Shander, Mazyar Javidroozi, Kevin M Trentino, Linda Shore Lesserson, Nelson Amaral, Caroline Evans, Irwin Gross, Jeannie Callum, Rawn Salenger, Daniel T Engelman, Patrick Meybohm, Pierre R Tibi

引用次数: 0

Transfusion-transmitted bacterial infection risk due to the proliferation of psychrotrophic bacterial species in RBCs and their difficulty in detection. 输血传播细菌感染的风险是由于红细胞中精神营养细菌种类的增殖和它们的检测困难。

IF 2.5 3区医学

Transfusion Pub Date : 2025-02-01 Epub Date: 2025-01-08 DOI: 10.1111/trf.18127

Moe Kozakai, Hideto Nagumo, Rika A Furuta, Keiji Matsubayashi, Masahiro Satake, Yoshihiko Tani

{"title":"Transfusion-transmitted bacterial infection risk due to the proliferation of psychrotrophic bacterial species in RBCs and their difficulty in detection.","authors":"Moe Kozakai, Hideto Nagumo, Rika A Furuta, Keiji Matsubayashi, Masahiro Satake, Yoshihiko Tani","doi":"10.1111/trf.18127","DOIUrl":"10.1111/trf.18127","url":null,"abstract":"Background: Reports of cases of bacterial infection due to transfusion of red blood cell (RBC) components (RBC-TTBI) are relatively rare. Hence, the possibility of undetectable bacterial contamination in RBCs, especially by psychrotrophic bacteria, must be clarified.Study design and methods: We assessed nine psychrotrophic bacterial species, including those implicated in bacteremia or RBC-TTBIs. They were cultured on plates from 4 to 37°C to determine their optimal growth temperatures. We also assessed the detection capabilities of the automated culture/alarm system BACT/ALERT VIRTUO (VIRTUO) using BPA (aerobic) and BPN (anaerobic) bottles. In addition, bacteria-inoculated RBCs were incubated at 4°C for 42 days, with samples assessed weekly for bacterial growth using plate culture, VIRTUO, visual inspection, and endotoxin production.Results: Two Psychrobacter species exhibited weak or no proliferation at temperatures ≥30°C in plate cultures. Three Pseudomonas species, one Psychrobacter species, and one psychrotrophic lactic acid bacteria proliferated in RBCs at 4°C, reaching 104-108 colony-forming units/mL (growth count) and 15-39,230 pg/mL (endotoxin production) by day 14. VIRTUO, operating at 36°C, failed to consistently yield reliable results for any of the tested bacterial species. Notably, visual changes in bag appearance were observed from day 21 in four species that proliferated in RBCs.Discussion: Each psychrotrophic bacteria demonstrated a specific temperature preference for optimal proliferation. Standard culture tests, typically conducted at 35-37°C, often fail to detect the growth of such bacteria, suggesting they may be overlooked in the cultural analysis of suspected RBC-TTBI cases.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"297-309"},"PeriodicalIF":2.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0