Transfusion最新文献

{"title":"Assessing the financial resources required to establish convalescent plasma collection programmes across Europe in response to a pandemic.","authors":"Elizabeth A Stokes, Abigail A Lamikanra, David J Roberts, Lise J Estcourt, Pierre Tiberghien, Jose Leal","doi":"10.1111/trf.70323","DOIUrl":"https://doi.org/10.1111/trf.70323","url":null,"abstract":"<p><strong>Background: </strong>During the COVID-19 pandemic, convalescent plasma (CP) was investigated as a potential treatment. We assess the financial resources required to implement a COVID-19 CP (CCP) programme and provide recommendations on how best to prepare for collection and use of CP in future pandemics.</p><p><strong>Study design and methods: </strong>We mapped pathways for collecting and manufacturing CCP using a UK blood establishment as a test case, and identified the resources required at each stage to provide high-titre (HT) CCP units. A top-down micro-costing approach and an economic model were used to establish the cost per HT CCP unit. Data collection questionnaires were later prepared to acquire data from 21 countries across Europe, facilitate comparisons and estimate costs per HT CCP unit using a top-down gross-costing approach. Costs are reported in Euros adjusted for cost-of-living differences.</p><p><strong>Results: </strong>The key cost driver for England was the cost of CCP collection. Nine countries responded, four with existing infrastructure for apheresis plasma collection at the start of the pandemic and five without. Four countries provided sufficient data to estimate the cost per HT CCP unit, which varied between €198-€265 for countries with pre-existing apheresis plasma collection and €807-€1749 for those without.</p><p><strong>Discussion: </strong>Countries had marked differences in the scale of CCP collection, and methods of selecting and measuring HT CCP, which contributed to the variation in expenditure observed. Costs were significantly lower in countries with existing infrastructure for apheresis plasma collection and where donors were tested for anti-SARS-CoV-2 antibody titre before attending plasma collection.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confirmatory testing of military blood donors screening positive for Babesia infection.","authors":"Siri Doddi, Megan L Phelps, Joseph E Marcus","doi":"10.1111/trf.70338","DOIUrl":"10.1111/trf.70338","url":null,"abstract":"<p><strong>Background: </strong>Babesia microti is a transfusion-transmitted pathogen. The US Food and Drug Administration (FDA) recommends blood donor screening only in endemic states, whereas the Military Health System performs universal Babesia screening regardless of geographic location. However, the epidemiology of Babesia positivity and clinical follow-up among blood donors in non-endemic regions remains poorly characterized.</p><p><strong>Study design and methods: </strong>We conducted a retrospective study evaluating Babesia screening results from three military blood donation centers in non-endemic states: Naval Base San Diego, Wright-Patterson Air Force Base, and Joint Base San Antonio-Lackland. All donations collected between January 1, 2021 and December 31, 2025 underwent nucleic acid testing using the Procleix Babesia Assay. Positive donors underwent retrospective chart review to assess demographics, confirmatory testing, clinical follow-up, and treatment outcomes.</p><p><strong>Results: </strong>Among 97,861 donations screened, eight donors tested positive for Babesia (8.17 per 100,000 donations). San Diego demonstrated the highest positivity rate (11.53 per 100,000), followed by Wright-Patterson (8.55 per 100,000) and San Antonio (5.82 per 100,000). Five donors were active-duty military personnel with medical records available for review, and three underwent follow-up clinical evaluation. Follow-up approaches varied considerably and include empiric treatment without confirmatory testing, antibody testing, peripheral blood smear evaluation, and polymerase chain reaction (PCR) testing. All clinically evaluated donors were asymptomatic, and there were no documented complications related to babesiosis.</p><p><strong>Discussion: </strong>Babesia positivity among military blood donors in non-endemic regions was rare. Variation in diagnostic evaluation and clinical management highlights the need for standardized follow-up protocols for military donors with positive screening results.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1916-1920"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148456957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Transfusion-related alpha-gal syndrome: Two new cases expanding the demographic and geographic spectrum, and evidence of a diagnostic gap in allergic transfusion reaction evaluation\".","authors":"","doi":"10.1111/trf.70345","DOIUrl":"10.1111/trf.70345","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"2067"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148550639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of washing on potassium leakage from red cells from individuals with familial pseudohyperkalemia.","authors":"Athinoula Meli, Christian J Stevens-Hernandez, Azhar Mohamudally, Kalpita Sakharkar, Helen V New, Rebecca Cardigan, Lesley J Bruce","doi":"10.1111/trf.70357","DOIUrl":"10.1111/trf.70357","url":null,"abstract":"<p><strong>Background: </strong>Familial pseudohyperkalemia (FP) is an asymptomatic condition characterized by an increased rate of potassium leak from red blood cells (RBC) on refrigeration. The effect of washing on FP RBC was investigated.</p><p><strong>Study design and methods: </strong>Ten FP and 20 non-FP individuals were identified from the National Institute for Health and Care Research BioResource. Whole blood was collected and held overnight at 20-24°C, leucocyte-depleted, and processed into red cell concentrates (RCC) in Saline Adenine Glucose Mannitol before cold storage at 4 ± 2°C. On Day 1, RCC were split into six red cell splits (RCS). Two RCS remained non-washed, two were washed on Day 2, and two were washed on Day 14. RBC were tested for quality parameters over storage.</p><p><strong>Results: </strong>Non-washed FP RCS had significantly higher supernatant potassium levels than non-FP throughout 28 days of storage (p < .001). When washed early, FP RCS released significantly higher potassium concentrations at Days 3 and 16 compared with non-FP; while when washed late, FP RCS released similar concentrations of potassium to control by Day 28. No other parameters studied showed a significant difference between FP and non-FP.</p><p><strong>Discussion: </strong>All refrigerated RBC leak potassium; however, FP RBC leak potassium and reach equilibrium more rapidly than non-FP. RBC washed on Day 2 still contained a high concentration of potassium and the differential rate of potassium loss between FP and non-FP RBC post-wash was as expected. By Day 14, FP RBC lost most of their intracellular potassium and the rate of potassium loss post-wash was reduced.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1974-1982"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148621468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three novel RHD variant alleles were identified through routine molecular screening of serologically RH1 negative first-time blood donors.","authors":"Kräuchi Rahel, Schimanski Bernd, Tinguely Caroline, Niederhauser Christoph, Lejon Crottet Sofia, Henny Christine","doi":"10.1111/trf.70352","DOIUrl":"10.1111/trf.70352","url":null,"abstract":"<p><strong>Background: </strong>The RH blood group system, particularly the RH1 (D) antigen, is highly immunogenic and clinically significant in transfusion medicine. Standard serological donor RH1 typing may fail to detect very weakly expressed RH1 variants, resulting in a risk of alloimmunization in recipients negative for the corresponding antigen. In 2012, routine molecular screening of RH1-negative first-time donors was implemented in Switzerland to improve transfusion safety. In this context, we present three novel RH1 variant alleles discovered through molecular analysis.</p><p><strong>Methods: </strong>Serological RH phenotyping was performed using column agglutination, adsorption-elution testing, and monoclonal antibody panels. Genomic DNA and RNA were extracted and analyzed by SSP-PCR, RHD exon sequencing, and cDNA sequencing.</p><p><strong>Results: </strong>Three previously undescribed RHD variant alleles were identified in four first-time donors. Two donors carried a nonsense mutation NM_016124.5:c.265C>T resulting in a premature stop codon (NP_057208.2:p.Gln89Ter), associated with an apparent RH1-negative phenotype but weakly positive adsorption-elution results. One donor carried a missense mutation NM_016124.5:c.863 T>A (NP_057208.2:p.Ile288Asn), associated with extremely weak RH1 expression. In one donor with a true RH1-negative phenotype, a frameshift mutation NM_016124.5:c.970del was identified, resulting in NP_057208.2:p.His324Thrfs*35 and predicted truncation of the RH1 protein.</p><p><strong>Discussion: </strong>The findings demonstrate that routine molecular RHD screening of serologically RH1-negative first-time donors is essential for the detection of rare RHD variants that may escape serological testing. Molecular characterization of such alleles improves transfusion safety by preventing inadvertent transfusion of RH1-positive red blood cells to RH1-negative recipients and contributes to a more comprehensive understanding of RHD genetic diversity.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1921-1925"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148713539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can hepatitis B surface antigen screening of blood donors be discontinued?","authors":"P N Lelie","doi":"10.1111/trf.70392","DOIUrl":"10.1111/trf.70392","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1881-1883"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Continuing Medical Education.","authors":"","doi":"10.1111/trf.70394","DOIUrl":"https://doi.org/10.1111/trf.70394","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"66 10","pages":"2007"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149196815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel missense mutation c.655G>A in the A4GALT*02 allele from a Chinese individual with p phenotype.","authors":"Jing Zhang, Ying Zhang, Yingjian Wang, Tiejun Song, Feng Dai, Shoufang Xu, Yingying Zhang, Feiyu Jiang, Zhiwei Liu","doi":"10.1111/trf.70329","DOIUrl":"10.1111/trf.70329","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"2049-2051"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clot contraction of whole blood diminishes during storage and can be restored by adding platelets.","authors":"Halen Turner, Monica Seadler, Youjie Zhang, Matteo Ferraresso, Christian Kastrup","doi":"10.1111/trf.70322","DOIUrl":"10.1111/trf.70322","url":null,"abstract":"<p><strong>Background: </strong>The contraction of blood clots is a dramatic platelet-dependent process that minimizes the impact of thrombosis and supports the closure of lacerations. Whole blood (WB) stored up to 35 days is increasingly used to treat hemorrhagic shock, but the clot contractibility of this stored blood is unknown. We hypothesized that clot contractibility decreases during storage but can be restored by adding platelets.</p><p><strong>Study design and methods: </strong>WB units from 6 donors were stored at 4°C for 35 days. Apheresis platelet units from 11 donors were stored for 5 days at 22°C with gentle oscillation. Clot contraction of WB and WB supplemented with platelets was measured daily in vitro using optical tracking. Platelets were added at a concentration of 30,000/μL.</p><p><strong>Results: </strong>Clot contraction of 6 WB samples diminished over 35 days of storage. The initial contraction rate and final contraction extent began declining at week 3. By week 5, the initial contraction rate was 67% slower, and the final contraction extent was 49% lower compared to week 1. Adding platelets at week 5 improved the initial contraction rate by 22% and the final contraction extent by 16%.</p><p><strong>Conclusion: </strong>WB stored at 4°C loses a portion of its clot contractility as early as 3 weeks of storage, which may affect patients receiving stored blood. The addition of room-temperature (RT) stored platelets can improve clot contraction, offering a potential strategy to enhance the effectiveness and safety of stored WB for hemorrhagic shock when fresh blood is unavailable.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"2008-2016"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148397869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downscaling to small-volume storage bags supports platelet quality and efficient use of cold-stored platelets for preclinical studies.","authors":"Janhavi Mahajan, Matthew P Padula, Denese C Marks, Lacey Johnson","doi":"10.1111/trf.70339","DOIUrl":"10.1111/trf.70339","url":null,"abstract":"<p><strong>Background: </strong>To maximize the utilization of cold-stored platelets (CSPs) in preclinical research, the use of small-volume storage bags (mini-bags) or tubes would allow multiple treatment groups to be investigated from a single component. It is necessary to determine the effect of using downscaled storage formats on CSP quality and function.</p><p><strong>Methods: </strong>Mini-bags were prepared by vacuum sealing platelet storage bags to ¼ the standard size. Apheresis platelets (n = 8) in 40% plasma/60% PAS-E (SSP+) were aseptically aliquoted into mini-bags (30 mL) or polypropylene tubes (15 mL; CSP-tubes), and the remaining volume (~190 mL) was retained in the original bag (control). Platelets were refrigerated (2-6°C), and in vitro quality parameters were assessed over 21 days.</p><p><strong>Results: </strong>Metabolic capacity was altered for CSP-tubes, with a significant decline in pH, mitochondrial membrane depolarization, and rapid glycolysis. CSP-tubes had greater storage-related activation, as evidenced by a higher proportion of annexin-V-positive platelets and a greater number of CD61+/annexin-V+ extracellular vesicles (EVs). Similarly, platelet functional capacity in CSP-tubes was compromised, with reduced clot strength compared to controls. CSPs stored in mini-bags remained comparable to controls, with the exception of a greater proportion of annexin-V-positive platelets and EVs at day 21. Importantly, despite these changes, no functional deficits were observed in mini-bag components.</p><p><strong>Conclusions: </strong>Storage in tubes markedly impairs the metabolic, structural, and functional quality of CSPs, indicating that this approach is unsuitable. In comparison, mini-bag storage preserves key in vitro parameters, supporting a scalable strategy that maximizes the utilization of CSPs for preclinical evaluation.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1952-1962"},"PeriodicalIF":2.0,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13674211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148685816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}