Transfusion最新文献

{"title":"Residual risk of human immunodeficiency virus, hepatitis C, and hepatitis B following implementation of sexual risk behavior-based screening in Canada.","authors":"Chinchin Wang, Steven J Drews, Mindy Goldman, Sheila F O'Brien","doi":"10.1111/trf.70244","DOIUrl":"https://doi.org/10.1111/trf.70244","url":null,"abstract":"<p><strong>Background: </strong>In 2022, Canadian Blood Services implemented a gender-neutral sexual behavior-based screening (SBBS) approach to donor deferral, replacing time-based criteria targeting gay, bisexual and other men who have sex with men (gbMSM) and female partners of gbMSM. We assessed the impact of this policy change on human immunodeficiency virus (HIV), hepatitis C (HCV), and hepatitis B (HBV) prevalence, incidence, and residual risk.</p><p><strong>Study design and methods: </strong>We compared prevalence rates among first-time donors, incidence rates, and residual risk for allogeneic donations made in the 3 years pre-SBBS implementation and 3 years post-SBBS implementation.</p><p><strong>Results: </strong>HIV and HBV prevalence remained steady while HCV prevalence increased among first-time donors post SBSS-implementation. Point estimates for incidence rates and residual risks decreased for each marker post-implementation, but differences were not significant. Residual risks post-implementation were 1 in 34.7 million for HIV, 1 in 64.1 million for HCV, and 1 in 3.1 million for HBV.</p><p><strong>Discussion: </strong>Residual risks of infection remain low following the implementation of a gender-neutral sexual risk behavior-based approach to donor deferral. This policy change has not negatively impacted the safety of the Canadian blood supply.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-apheresis CD34+ cell count: A dynamic approach to predicting peripheral blood stem cell collection yield.","authors":"Abdullah Alswied, Linda Song, Mame Thioye-Sall, Leonard N Chen, Kamille West-Mitchell, Nan Zhang, Ping Jin, David Stroncek, Anh Dinh","doi":"10.1111/trf.70225","DOIUrl":"https://doi.org/10.1111/trf.70225","url":null,"abstract":"<p><strong>Background: </strong>Accurate prediction of peripheral blood stem cell (PBSC) yield is essential for optimizing hematopoietic stem cell collections. Traditional CD34+ prediction formulas often overlook the ongoing recruitment of CD34+ cells during the collection procedure. This study evaluated whether a single intra-apheresis CD34+ measurement could improve yield prediction compared to existing prediction models.</p><p><strong>Methods: </strong>We retrospectively analyzed 150 PBSC collections from 123 autologous and allogenic donors. A single intraprocedural CD34⁺ measurement and final collection yield were obtained, and univariable and multivariable linear regressions were developed to assess predictive accuracy. Model stability and generalizability were tested via bootstrap resampling and cross-validation. Subgroup analyses by age, sex, weight, diagnosis, and sampling time were performed to confirm robustness. We also compared our results against published pre-apheresis formulas.</p><p><strong>Results: </strong>Intra-apheresis CD34⁺ counts showed a strong correlation with the final yield (r = 0.97; R² = 0.95). Subgroup analyses across donor demographics and clinical factors yielded similarly high R² values (0.91-0.99). Multivariable analysis identified intra-apheresis CD34+ measurement as the primary contributor (β = 0.67, p <.0001). Later sampling (≥1.20 of total blood volumes processed) reduced error metrics relative to earlier draws. Compared to pre-apheresis models, the intra-apheresis approach achieved higher R² and lower error metrics.</p><p><strong>Discussion: </strong>A single intra-apheresis CD34+ measurement, particularly at or beyond 1.20 processed blood volumes, offers a reliable, real-time predictor of final PBSC yield. This intraprocedural approach outperforms published pre-apheresis formulas, enabling more efficient collection and reduced donor burden across diverse clinical scenarios.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New RHCE*cE allele with c.872C>G and variable RH3 and RH4 phenotypes, predicted to encode p.Pro291Arg.","authors":"Evelyne Heng, Gabriel Neto Braga, Marine Branger, Mylene Flammang, Christophe Tournamille, Aline Floch","doi":"10.1111/trf.70237","DOIUrl":"https://doi.org/10.1111/trf.70237","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity in RHD and RHCE genes among a selected Kenyan blood donor population.","authors":"Sandra A Sowah, Alexis J Perros, Rachel Githiomi, Genghis H Lopez, Celestino Obiero, Thilini N Perera, Eileen Roulis, Robert L Flower, Catherine A Hyland","doi":"10.1111/trf.70205","DOIUrl":"https://doi.org/10.1111/trf.70205","url":null,"abstract":"<p><strong>Background: </strong>Serologic typing for ABO and RhD is standard in transfusion services, with extended serology and genotyping performed to reduce red cell alloimmunization risk. In Kenya, RH typing is limited to RhD, and genotyping is unavailable. This study used RHD/RHCE genotyping to predict phenotypes and their distribution in a Kenyan blood donor population.</p><p><strong>Study design and methods: </strong>A total of 191 donors (114 D-, 74 D+, and 3 weak D) from the Kenya National Blood Transfusion Service were selected. Next-generation sequencing was performed on DNA extracts using a targeted blood group sequencing panel (Illumina MiSeq). Variant call format (VCF) files were annotated with wANNOVAR, and phenotypes were predicted by matching VCF data to the International Society of Blood Transfusion (ISBT) Blood Group database.</p><p><strong>Results: </strong>RHD*01N.01, RHD*08N.01 (RHD*Ψ), and RHD*03N.01 alleles were identified predicting D- phenotype. Discordant phenotype results were observed in 11 samples with genotype predicting nine D+ (partial D) in 114 D-, one D- in 74 D+, and one D- in three weak D phenotypes. For RHCE, 15 allele types produced 30 genotypes with 63% carrying at least one RHCE variant allele linked to: 1) weak and/or partial c and e, 2) hr^B-, and 3) V+/-, VS+, phenotypes.</p><p><strong>Discussion: </strong>Genotyping revealed RhD/RHD phenotype/genotype discrepancies and RH allele diversity among Kenyan donors, including RHCE variants affecting high- and low-prevalence antigen expression. These findings highlight the role of genotyping to improve accuracy for RH typing to minimize the risk of patient alloimmunization.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of implementation of ferritin testing on donor return.","authors":"Mindy Goldman, Chinchin Wang, Aditi Khandelwal, Sheila F O'Brien","doi":"10.1111/trf.70236","DOIUrl":"https://doi.org/10.1111/trf.70236","url":null,"abstract":"<p><strong>Background: </strong>One mitigation strategy for donor iron deficiency is to implement ferritin testing and encourage donors with low ferritin to pause donation and increase iron intake. We evaluated positive outcomes (reduced hemoglobin deferrals) and negative outcomes (donor nonreturn) in a 2-year observation period after implementing ferritin testing of female donors at each 10th donation.</p><p><strong>Methods: </strong>Donors with ferritin below 25 ug/L were advised to pause donation for 6 months and see their health care practitioner about increasing iron intake but were not deferred. Ferritin results for donors screened in the first 6 months of the program (Jan-June 2023), hemoglobin on return and time to return in the 2 years following testing were calculated for low ferritin and normal/high ferritin donors.</p><p><strong>Results: </strong>Twenty five percent of 8613 tested donors had low ferritin, associated with younger age, high donation frequency, and previous hemoglobin deferral. About one third of low ferritin donors returned to donate less than 6 months after testing, and had a hemoglobin deferral rate of 16%, compared to 4.4% in those who waited 6 months or more and 5.2% in donors with normal ferritin. After 2 years, 77.5% of low ferritin compared to 88.5% of normal/high ferritin donors returned to donate.</p><p><strong>Conclusion: </strong>Iron deficiency was common. Low ferritin donors who paused donation and returned to donate had hemoglobin deferral rates similar to normal ferritin donors. However, many returned early and had high failure rates. Two years after testing, an 11% difference in return rates remained between low and normal/high ferritin donors.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147782135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low titer group O whole blood inventory conservation: The effect of implementing group A whole blood at a simulated civilian trauma center.","authors":"Emilie A Jochumsen, Jennifer Gurney, Donald Jenkins, Jansen N Seheult, Philip C Spinella, Ulrik Sprogøe, Mark H Yazer","doi":"10.1111/trf.70235","DOIUrl":"https://doi.org/10.1111/trf.70235","url":null,"abstract":"<p><strong>Background: </strong>Substituting group A whole blood (GAWB) might relieve some of the burden on the low titer group O whole blood (LTOWB) inventory. This simulation examined the effect of implementing GAWB at a large trauma hospital.</p><p><strong>Study design and methods: </strong>A civilian trauma center with LTOWB and GAWB par levels set to 22 and 10 units, respectively, was modeled as the baseline. The average daily number of WB recipients was 0.5 who received an average of four WB units per patient. LTOWB was issued in packages of five and all had to be consumed before a group A patient could be switched to GAWB. Several variations on the baseline simulation were also modeled.</p><p><strong>Results: </strong>In the baseline simulation, there was a median (interquartile range, IQR) of 1 (0-2) GAWB and 16 (13-19) LTOWB recipients per month. The median (IQR) GAWB and LTOWB units transfused per month was 2 (0-9) and 56 (42-72), respectively. Fourteen percent of group A patients received any quantity of GAWB. The variations that had the most impact on GAWB usage included increasing the number of WB recipients to 2.5 per day where the median (IQR) monthly number of GAWB recipients and units transfused increased to 4 (3-5) recipients and 21 (12-29) units, respectively, and when the average WB requirements increased to eight units per patient resulting in transfusing a median (IQR) of 10 (5-15) GAWB units per month to 3 (1-4) recipients.</p><p><strong>Discussion: </strong>Under routine civilian hospital circumstances, implementing GAWB will have a modest impact on LTOWB inventory.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147718051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel RHD*49C allele identified in a prenatal patient.","authors":"Adam Norfolk, Lynsi Rahorst, Sunitha Vege, Alexandra Kern, Rose Little, June Fuertes, Catherine Chadbourne, Mark Steciuk, Gorka Ochoa-Garay","doi":"10.1111/trf.70171","DOIUrl":"https://doi.org/10.1111/trf.70171","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel RHCE*cE(341A) allele.","authors":"Rébecca Voreux, Sunitha Vege, Odile Crépin, Raynald Flahaut, Céline Ricard, Gorka Ochoa-Garay, Chloe Homich, Bruce S Sachais, Lynsi Rahorst, Randall W Velliquette, Aurélie Barrault, Christophe Tournamille, Aline Floch","doi":"10.1111/trf.70204","DOIUrl":"https://doi.org/10.1111/trf.70204","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful re-infusion of hepatitis E contaminated stem cells during autologous stem cell transplantation with ribavirin prophylaxis.","authors":"Hannah Sadler, Nicola Knightly, Isobelle Waite, Hayley Aplin, Alexander David Mangan, Susan Austin, Tiffany Chan, Rezarta Fekollari, Ines Ushiro-Lumb, Laura Kent, Moby Joseph, Samreen Ijaz, John Griffith, Marcin Lubowiecki","doi":"10.1111/trf.70228","DOIUrl":"https://doi.org/10.1111/trf.70228","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis E virus (HEV) infection is a common and usually asymptomatic infection but can be fatal in the context of prolonged immune suppression, such as during a hematopoietic stem cell transplant (HSCT).</p><p><strong>Case report: </strong>We describe the case of a 74-year-old man with primary central nervous system lymphoma (PCNSL) who required an autologous stem cell transplant (ASCT) as part of curative treatment for this condition but was identified to have acute HEV infection at the time of CD34 stem cell harvest. This meant he would be re-exposed to a contaminated product with HEV infection at high viral load precisely at the time of most severe immune suppression.</p><p><strong>Results: </strong>This is the first case of the prospective use of a CD34-contaminated hepatitis E stem cell product with a high viral load (2,950,000 IU/mL) in an autologous stem cell transplant setting. We describe the successful prophylaxis with ribavirin. This case provides a practical management strategy for a HSCT, particularly in the allogeneic setting, where time-critical donor CD34 products are required where acute HEV infection is detected. Our patient remains alive at >12 months post-transplant with no evidence of HEV re-activation.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycemic control and blood product quality of Canadian blood donors with diabetes.","authors":"Elyn M Rowe, Marcus Shew, Noor Ali-Mohamad, Celina Phan, Mindy Goldman, Jason P Acker, Dana V Devine, James D Johnson","doi":"10.1111/trf.70223","DOIUrl":"https://doi.org/10.1111/trf.70223","url":null,"abstract":"<p><strong>Background: </strong>Many blood centers now accept donors with diabetes, provided their condition is \"well-controlled\". However, glycemic control is not routinely assessed at donation, and the impact of donor diabetes on blood product quality remains unclear.</p><p><strong>Study design and methods: </strong>To screen glycemic control, glycated hemoglobin A1c (HbA1c) was measured in whole blood from 256 unique donors with either type 1 diabetes (T1D), type 2 diabetes (T2D), or without known diabetes. In parallel, routine quality control (QC) data were linked with donor metadata to assess the impact of donor diabetes on red cell concentrate (RCC) and apheresis platelet concentrate (PC) quality at the time of their expiry.</p><p><strong>Results: </strong>Nearly half of donors with diabetes had suboptimal glycemic control (HbA1c >7 %) at donation, including 57.1 % of donors with T1D and 43.4 % with T2D. RCC units from donors with T2D (n = 1,329) had higher hemolysis (p <.001) and a greater proportion exceeding the clinical threshold of 0.8 % hemolysis (p <.001). These differences were not observed with T1D (n = 46). Apheresis PC units from donors with T2D (n = 164) had lower expiry pH (p = .014), with a trend toward more units below the clinical cutoff of pH 6.4 (p = .059). Differences in both products were most pronounced in donors with T2D using both metformin and insulin.</p><p><strong>Conclusion: </strong>Many donors with diabetes have suboptimal glycemic control, and RCC and PC from donors with T2D have reduced quality. The observed effects are modest, likely not driven by glycemic control alone, and do not support additional deferral criteria for donors with diabetes.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147692461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}