Transfusion最新文献

{"title":"Preparation of cold-stored platelets on-demand: A novel approach to inventory management.","authors":"Lacey Johnson, Lauren Waters, Christopher Roan, Pearl Lei, Janhavi Mahajan, Mailys Portier, Ben Winskel-Wood, Denese C Marks","doi":"10.1111/trf.18396","DOIUrl":"https://doi.org/10.1111/trf.18396","url":null,"abstract":"<p><strong>Background: </strong>Cold-stored platelets (CSP) are now being used to treat acute bleeding. However, as CSP are less suitable for prophylaxis, both room temperature (RT) platelets and CSP will be required, which complicates inventory management. The production of CSP \"on-demand\" from RT platelets may be a desirable option. This study investigated whether CSP could be prepared rapidly by cooling RT platelets in a fridge or freezer.</p><p><strong>Study design and methods: </strong>Double apheresis platelet components (40% plasma/60% PAS-E) were sampled on Day 1 (RT baseline), then split into matched pairs (n = 10). One component was allocated to cold (2-6°C) storage and sampled at 2 h and 14 days. The other component was placed in a freezer (-30°C) and sampled every 5 min for 20 min (on-demand), then transferred to the fridge and sampled at 14 days.</p><p><strong>Results: </strong>Storage in the fridge for 2 h reduced the temperature to 4.1 ± 0.7°C and induced loss of swirl, shape change, enhanced aggregation, and reduced clotting time. Changes to surface glycoproteins (CD61, CD42b, GPVI) and activation markers (phosphatidylserine, P-selectin, release of alpha-granule proteins, and extracellular vesicles) were not observed after 2 h but became evident after 14 days. Storage in the freezer for at least 15 min induced the expected \"cold storage lesion\" changes.</p><p><strong>Discussion: </strong>The morphological and functional characteristics of CSP can be induced within 15 min by exposure to a standard blood-bank freezer, or 2 h in the fridge. This approach to prepare CSP \"on-demand\" may represent a viable option for balancing patient-tailored transfusion and inventory management.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How do I implement a whole blood program with low blood wastage?","authors":"Denise Bäckström, Malin Jonsson Fagerlund, Stella Larsson, Aseel Alshamari, Ragnar Henningsson, Karin Holst, Anna-Maria Harstad, Norbert Lubenow, José-Gabriel Sato Folatre, Pia Remneberg Carlbom, Agneta Wikman","doi":"10.1111/trf.18402","DOIUrl":"https://doi.org/10.1111/trf.18402","url":null,"abstract":"<p><strong>Background: </strong>The use of low-titer O whole blood (LTOWB) is requested in the treatment of major bleeding, initially used in military medicine but now increasingly utilized in civilian prehospital care. The advantage is the administration of a balanced transfusion, red blood cells, coagulation factors, and platelets, in one bag. The challenges are the availability of LTOWB and difficulties in predicting the need in major bleeding, leading to the risk of wastage.</p><p><strong>Methods: </strong>This study describes different logistical strategies when implementing whole blood in the Swedish civilian healthcare. The five transfusion centers producing whole blood in Sweden participated, providing experience of the production line, usage, and wastage.</p><p><strong>Results: </strong>In Sweden, LTOWB is used prehospital in helicopter emergency medical service (HEMS), in one physician-manned rapid response vehicle, and inhospital in three University Hospitals. The logistical strategies to reduce wastage vary but involve the rotation of LTOWB not used prehospital to inhospital use in two centers and the preparation of red blood cell (RBC) units from 1 to 2 weeks old LTOWB in three centers. The number of transfused LTOWB units varies between the centers, and wastage was 0%-13% in 4/5 centers and higher in one center, 34%.</p><p><strong>Conclusion: </strong>It is difficult to predict the need of LTOWB, requested in prehospital emergencies. Aiming for low wastage requires different logistical chains, depending on the local prerequisites. In Sweden, LTOWB is either rotated for use in major bleeding in hospital or prepared to RBC units after 1 week prehospital.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel RHD*01N allele harboring a c.634+1G>A splice site variant results in the RhD-negative phenotype in a Chinese blood donor.","authors":"Xu Zhang, Zhu-Ren Zhou, Xu-Ying Huang, Li-Chun Li, Xiao-Feng Li, Jian-Ping Li","doi":"10.1111/trf.18390","DOIUrl":"https://doi.org/10.1111/trf.18390","url":null,"abstract":"<p><strong>Background: </strong>The D-negative phenotype demonstrates significant ethnic diversity in its molecular background. This study reports the identification of a novel RHD*01 N allele resulting from a splicing site variation observed in a Chinese blood donor.</p><p><strong>Study design and methods: </strong>The D blood group phenotype was determined using serological techniques, including the saline method, and the indirect antiglobulin test (IAT) performed by both tube and microcolumn gel methods. Screening for the RHD gene was performed by directly amplifying exon 10 of the RHD gene from whole blood samples. Upon confirming the presence of the RHD gene in the sample, further detailed analysis was performed using Sanger sequencing and third-generation single-molecule real-time (SMRT) sequencing technologies. Additionally, the deep learning-based tool SpliceAI (Illumina, USA) was used to evaluate the potential impact of an unreported variant on splicing.</p><p><strong>Results: </strong>The D antigen in the proband's blood sample was detected as negative by both automated and manual methods. Exon 10 of the RHD gene was detected as positive. Sanger sequencing and third-generation single-molecule real-time (SMRT) sequencing were utilized for the detection of the RHD gene, and the results demonstrated that the sample carried a single RHD haplotype harboring a c.634+1G>A splice site variant. The SpliceAI prediction indicates that the c.634+1G>A splice site variant has a significant impact on splicing.</p><p><strong>Conclusion: </strong>A novel RHD allele harboring the c.634+1G>A splice site variant, which results in the RhD-negative phenotype, was identified and characterized in a Chinese blood donor.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145001089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of platelet transfusions on plasma proteomes in controlled endotoxemia and in hemato-oncological patients.","authors":"Eva R Smit, Isabella Viegen, Pieter F van der Meer, Stefan F van Wonderen, Floor L F van Baarle, Paula F Ypma, Jean-Louis H Kerkhoffs, Jan Voorberg, Alexander P J Vlaar, Anna L Peters, Maartje van den Biggelaar, Diana Muñoz Sandoval","doi":"10.1111/trf.18397","DOIUrl":"https://doi.org/10.1111/trf.18397","url":null,"abstract":"<p><strong>Background: </strong>Inflammation is a hallmark of patients that receive platelet transfusions, including critically ill and hemato-oncological patients. Platelet transfusions have been suggested to exacerbate inflammatory conditions, resulting in transfusion-related complications. Here, we used plasma proteomics to study the impact of platelet transfusions under inflammatory conditions.</p><p><strong>Methods: </strong>Plasma proteomics was performed in samples from two studies: (1) 252 samples from healthy volunteers from 36 cases divided into groups (n = 6) with and without controlled endotoxemia, each with different platelet products, and (2) 54 samples from 27 transfusion events in hemato-oncological patients (n = 14). Both studies had samples pre- and post-transfusions.</p><p><strong>Results: </strong>Controlled endotoxemia elicited a shared inflammatory response across all healthy volunteers. This response was characterized by increased abundance of two kinetically distinct protein clusters, an initial cluster associated with degranulation and inflammation (i.e., S100A9 and MMP9) followed by a cluster of acute-phase proteins (i.e., SAA and CRP). Autologous platelet transfusions in healthy individuals did not induce transfusion-specific changes in plasma protein levels, nor did they exacerbate the effect of controlled endotoxemia. In hemato-oncological patients, we found a transfusion-specific response restricted to alterations in platelet basic protein (PPBP) levels. No additional changes in plasma protein profiles associated with inflammation or dysregulated processes were observed following platelet transfusions.</p><p><strong>Discussion: </strong>Platelet transfusions did not induce specific quantitative changes in plasma protein levels in healthy volunteers. Nonetheless, they were associated with increased levels of PPBP in hemato-oncological patients. In general, no evidence of inflammation or other dysregulated processes was observed following platelet transfusions.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In response: Approach to autologous stem cell harvesting and transplantation in patients with cold-agglutinin disease or cryoglobulins.","authors":"Rachel Pawson, Steve Bowen, Gurman Kaur","doi":"10.1111/trf.18395","DOIUrl":"10.1111/trf.18395","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nonsense variant c.322C>T in B allele leads to ABO discrepancy in a Chinese stem cell donor.","authors":"Ning Li, Linnan Shao, Yicheng Yang, Shihang Zhou, Xiaohua Liang","doi":"10.1111/trf.18339","DOIUrl":"https://doi.org/10.1111/trf.18339","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"E46-E48"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing hepatitis B virus infectivity in blood components following pathogen reduction using a human hepatocyte model.","authors":"Bryan J Visser, Santanu Biswas, Rana Eltahan, Rafaelle Fares-Gusmao, Subramanian Yegneswaran, Nina Mufti, Carlos H Villa, David R McGivern","doi":"10.1111/trf.18357","DOIUrl":"10.1111/trf.18357","url":null,"abstract":"<p><strong>Background: </strong>Pathogen reduction technologies (PRTs) have the potential to reduce the risk of emerging transfusion transmissible infections. Evaluating PRT activity against hepatitis B virus (HBV) presents some unique challenges due to the lack of robust model systems. Surrogate viruses (e.g., duck HBV) can be used, but may differ from the human pathogen in ways that influence susceptibility to a given PRT.</p><p><strong>Methods: </strong>Whole blood (WB) collected from volunteers was spiked with human plasma from deferred HBV-positive donors. Spiked WB was then treated with the nucleic acid crosslinking compound S-303 or left untreated. Additional physical and chemical treatments were also assessed. Plasma prepared from the spiked WB was used to inoculate human hepatocyte cultures isolated from chimeric mice with humanized livers. Culture medium was monitored over time for hepatitis B surface antigen (HBsAg) and viral DNA. Cell lysates were analyzed for evidence of covalently closed circular (ccc) DNA.</p><p><strong>Results: </strong>The culture system faithfully measured infectious HBV in blood components. S-303 treatment of HBV-spiked WB prevented infection of hepatocyte cultures, as monitored by HBsAg expression, at concentrations as low as 0.125 mM of S-303 or with incubation times as short as 1 h. Additionally, S-303 treatment prevented the accumulation of T5 exonuclease-resistant HBV DNA in inoculated hepatocytes, suggesting that S-303 prevents establishment of HBV cccDNA.</p><p><strong>Conclusion: </strong>This model mimics transfusion transmission by using donor-derived HBV and mouse-passaged human hepatocytes, allowing evaluation of the efficacy of different PRTs against authentic human HBV isolates. We found S-303 to be a potent inactivator of HBV.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1673-1681"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variants in the CD59 gene: An exploratory study of large genome databases.","authors":"Kshitij Srivastava, Thomas Christopher Recupero, Willy Albert Flegel","doi":"10.1111/trf.18331","DOIUrl":"10.1111/trf.18331","url":null,"abstract":"<p><strong>Background: </strong>CD59 is a GPI-anchored glycoprotein on the surface of many cell types. It inhibits the assembly of the membrane attack complex, thus preventing complement-mediated cell lysis. Lack of a functional CD59 protein causes recurrent ischemic strokes, neuropathy, and chronic hemolysis. We aimed at a comprehensive analysis of the CD59 gene from publicly available databases to identify variants and evaluate their pathophysiologic potential.</p><p><strong>Methods: </strong>Variants in the CD59 coding sequence of exons 4, 5, and 6 and their splice sites were systematically compiled from 4 major populations across 6 whole-genome and whole-exome databases. The PredictSNP algorithm assessed the functional impact of non-synonymous variants.</p><p><strong>Results: </strong>Among 488,592 individuals, 160 distinct alleles were identified in 6881 subjects (0.7%). Among 93 alleles with non-synonymous variants, 43 were classified as deleterious and 49 (2 variants encoded the same amino acid change) as neutral by PredictSNP. Additional variants included: 53 synonymous, 9 deletions/duplications, 3 splice site, and 2 nonsense. Among the 14 non-synonymous variants reported in patient samples, 9 were classified as deleterious (64.3%) and 5 as neutral (35.7%).</p><p><strong>Discussion: </strong>We collated a list of CD59 variants, which were classified as neutral or deleterious by PredictSNP, from genome databases. These results can be applied to identify individuals with possible latent CD59 deficiency symptoms, such as hemolytic transfusion reactions. In conjunction with clinical data, these CD59 variants can guide personalized clinical decisions.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1682-1692"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144754390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered DNA methylation of the ABO gene is associated with differential plasma levels of von willebrand factor and E-selectin.","authors":"Tianai Lou, Jamie A Sugrue, Etienne Patin, Lluis Quintana-Murci, Darragh Duffy, Cliona O'Farrelly","doi":"10.1111/trf.18342","DOIUrl":"10.1111/trf.18342","url":null,"abstract":"<p><strong>Background: </strong>The ABO blood group system is associated with differential susceptibility to thrombotic vascular diseases. ABO is also known to be a strong trans-protein quantitative trait locus for plasma proteins involved in cell adhesion and hemostasis.</p><p><strong>Study design and method: </strong>To further investigate these associations, we integrated epigenomic, genomic, and proteomic data from the Milieu Intérieur cohort. We used the rs8176719 SNP to classify donors as either type O or non-O, and used linear models to compare levels of 229 plasma proteins in 400 donors, including age, sex, cytomegalovirus serostatus, and secretor status as covariates.</p><p><strong>Results: </strong>We observed increased levels of soluble E-selectin and decreased levels of von Willebrand Factor (vWF) in O donors compared with non-O donors. By performing an epigenome-wide association study, we identified 23 differentially methylated CpG sites between blood types, which were all located in the ABO gene. Notably, CpG sites in the ABO promoter region of type O donors were less methylated than those of the non-O donors. Using mediation analysis, we found that these differences in DNA methylation partially explained the effects of blood group on differential E-selectin and vWF plasma levels.</p><p><strong>Discussion: </strong>We find differentially methylated CpG sites between blood types and provide new evidence that ABO blood group status affects circulating levels of specific proteins.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1693-1706"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432810/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogen-reduced cryoprecipitate: Early experiences and inventory management.","authors":"C B Webb, A Javanbakht, D Hanna, S G Yates, R Zhang, S Afraz, R Sarode","doi":"10.1111/trf.18344","DOIUrl":"10.1111/trf.18344","url":null,"abstract":"<p><strong>Background: </strong>Room temperature storage of cryoprecipitated antihemophilic factor (CryoAHF) is limited to 6 h. Consequently, this product is stored frozen until requested, necessitating thawing and delaying fibrinogen supplementation. The FDA has approved the use of pathogen-reduced cryoprecipitated fibrinogen complex (PRCFC), which may be stored at room temperature up to 5 days. Opportunities for rapid dispensing of PRCFC and decreasing wastage were identified.</p><p><strong>Methods: </strong>Our institution implemented PRCFC in January 2022. Two to eight pools (1-4 doses) of PRCFC were maintained thawed at 20°C-24°C for rapid dispensing. A retrospective analysis of the turnaround time (TAT) to prepare and issue CryoAHF versus PRCFC orders was performed for orders between January 2022 and December 2024. Orders exceeding 60 min to issue were excluded from analysis as these reflected non-urgent orders or cryoprecipitate prepared in advance as part of massive transfusion protocols not released until later shipments.</p><p><strong>Results: </strong>PRCFC was used in 40.3% of cryoprecipitate orders placed. Comparing PRCFC and CryoAHF, TAT reduction was significant for orders placed from the operating room, labor and delivery, and intensive care units, with reductions in time to prepare and issue of up to 50.0% and 47.7%, respectively (p < 0.001; p < 0.001). The longer shelf life of PRCFC led to a 54.2% reduction in cryoprecipitate wastage from 2021 to 2024.</p><p><strong>Conclusion: </strong>PRCFC is ready to dispense and provides an immediate source of fibrinogen in critically bleeding patients with significant reductions in TAT. There is an added benefit of reduced wastage.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"1580-1585"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}