Transfusion最新文献

{"title":"2025 Abstract Reviewers.","authors":"","doi":"10.1111/trf.18421","DOIUrl":"https://doi.org/10.1111/trf.18421","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 Suppl 2 ","pages":"4A-5A"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence and timing of seroconversion during pregnancy-A retrospective study to enable safe transfusion.","authors":"E Dreyfuss, V Yahalom, Y Barer, I Ambar, A Pardo, A Shmueli, O Houri, E Hadar, S Barbash-Hazan","doi":"10.1111/trf.18356","DOIUrl":"10.1111/trf.18356","url":null,"abstract":"<p><strong>Background: </strong>During pregnancy, exposure to fetal red blood cell antigens can trigger alloimmunization. To enable safe transfusion of compatible blood for pregnant individuals where urgent surgery might be necessary, blood bank standards mandate a valid blood type and antibody screening every 72 h (\"3-day rule\"). This practice requires frequent blood drawings contributing to patient discomfort and costs. To investigate if this practice is required, we evaluated the proportion of pregnant individuals who develop clinically significant alloantibodies within 72 h of a previous negative antibody screen.</p><p><strong>Study design and methods: </strong>Retrospective cohort of RhD-positive pregnant individuals with initial negative antibody screens, undergoing repeated screens at variable intervals. Seroconversion rates were compared with healthy male blood donors. Characteristics of pregnant individuals with and without seroconversion were analyzed.</p><p><strong>Results: </strong>Among 8659 RhD-positive pregnant individuals with initial negative antibody screens, 56 (0.6%) converted to a positive antibody screen, while only 15 (0.2%) developed clinically significant alloantibodies. Of these 15, only one converted within 3 days of testing, while others converted within 5-95 days. Two of the 15 women received a blood transfusion, 8-97 days before seroconversion. No male donors seroconverted during 9 months of follow up. Pregnant individuals with seroconversion were more likely to have systemic lupus erythematosus and antiphospholipid antibody syndrome.</p><p><strong>Discussion: </strong>Only one pregnant individual developed clinically significant alloantibodies within 72 h of a negative screening, compared to none of the control group. For pregnant individuals without recent transfusion, extending antibody screening intervals to 1 week appears safe.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1707-1715"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2025 Oral abstract presentation schedule.","authors":"","doi":"10.1111/trf.18419","DOIUrl":"https://doi.org/10.1111/trf.18419","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 Suppl 2 ","pages":"1A-2A"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145201530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent overestimation of IgM antibody titers by IgG in the column agglutination technique.","authors":"Hayato Kojima, Hideaki Matsuura, Yuko Abe, Ayuna Yamada, Yasuo Miura","doi":"10.1111/trf.18340","DOIUrl":"10.1111/trf.18340","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1561-1563"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144668589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A patient with anti-Co^a having activated monocytes specifically phagocytosing IAT-negative Co(a+) red blood cells.","authors":"Yeniley Ruiz Noa, Rosanne M St Bernard, Jordan Radigan, Matthew T S Yan, Donald R Branch","doi":"10.1111/trf.18355","DOIUrl":"10.1111/trf.18355","url":null,"abstract":"<p><strong>Background: </strong>The antigen, Co^a(CO1), is a high prevalence antigen. There is not a substantial amount of evidence regarding the clinical significance of anti-Co^a, especially if this antibody can cause phagocytosis. Using the monocyte monolayer assay (MMA), we have investigated the potential clinical significance of anti-Co^a in a patient that appeared to have an activated mononuclear phagocyte system.</p><p><strong>Study design and methods: </strong>A female, AB RhD-negative patient having anti-Co^a was investigated for the potential clinical significance of the antibody using the MMA. On initial presentation, MMA using both autologous and allogeneic monocytes were performed using the following red blood cell (RBC) samples: four Co(a+), one Co(a-) autologous RBC, a positive control of RhD-positive RBC opsonized with anti-D and a nonopsonized RhD-negative control. The patient's plasma was tested against all RBC samples by a saline-IAT (sIAT). A DAT on the patient's RBC was performed using polyspecific and monospecific antihuman globulin sera The autologous MMA was performed initially on presentation and again, 2 months later.</p><p><strong>Results: </strong>Initial autologous MMA showed significant phagocytosis with all 4 Co(a+) donors, despite the sIAT being nonreactive with both IgG and C3d. The RBCs from the Co(a-) donor did not show any phagocytosis. The patient had a negative tube DAT, however, was able to significantly phagocytize their own RBCs. Anti-D-opsonized RhD-positive RBCs showed greatly enhanced phagocytosis compared to what is usually observed. Allogeneic MMA showed no significant phagocytosis. Follow-up autologous MMA, 2 months later, when the patient was stable having a hemoglobin of 15.5 g/dL, showed similar results as the initial MMA using the dame donor RBCs as in initial testing; however, the patient's monocytes no longer gave a positive result with autologous RBCs.</p><p><strong>Discussion: </strong>These results suggest an enhanced activation of the patient's monocytes that is specific for Co(a+) RBCs and indicate the anti-Co^a as potentially clinically significant.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"1592-1598"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a novel ABO allele c.98+1G>A in a Chinese individual using PacBio third-generation sequencing.","authors":"Lin-Nan Shao, Yue-Xin Xia, Shi-Hang Zhou, Xiao-Hua Liang","doi":"10.1111/trf.18361","DOIUrl":"https://doi.org/10.1111/trf.18361","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"E52-E54"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfusion Camp for medical students in Rwanda: A multidisciplinary initiative teaching graduating medical students how to utilize blood products and derivatives safely in district hospitals.","authors":"McKenna Postles, Teresa Skelton, Jacob Pendergrast, Aggrey Dhabangi, Yulia Lin, Aimable Kanyamuhunga","doi":"10.1111/trf.18337","DOIUrl":"10.1111/trf.18337","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1761-1763"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144627160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility and safety of peripheral blood stem cell collection in children with extremely low body weight: A single center study.","authors":"S Grewal, R Hassanein, S Mendoza, E Sajdak, H C Sullivan, R Jacob","doi":"10.1111/trf.18345","DOIUrl":"10.1111/trf.18345","url":null,"abstract":"<p><strong>Background: </strong>Apheresis cell collections (HPC(A)) are used for hematopoietic stem cell transplantation and gene therapy; however, they present unique challenges in children with extremely low body weight (≤10 kg). We aimed to investigate the feasibility and safety of HPC(A) in these patients.</p><p><strong>Study design and methods: </strong>This retrospective single-center study reviewed HPC(A) collections at one pediatric center between 2017 and 2024 in patients ≤10 kg. Data included collection parameters, anticoagulant type, and demographics. Feasibility was assessed on target CD34+ count. Safety was evaluated on the incidence and severity of adverse events (AEs). Descriptive statistics and comparative tests (Wilcoxon rank sum, Welch t-test) were applied.</p><p><strong>Results: </strong>Nineteen patients underwent 20 autologous collections with an average patient weight of 7.70 kg. Central venous access was required in all, with 90% inpatient collections. Average patient total blood volume and volume processed were 557 and 2523 mL. Collections used acid citrate dextrose, solution A (ACD-A) only (50%) or ACD-A + heparin (50%). Run times between the two were not significantly different, but the inlet flow rate was significantly higher in the ACD-A + heparin group (p = .017). 90% of patients received granulocyte colony-stimulating factor only, with the remainder also receiving plerixafor. Average minimum-target dose was 12.8-20.5 million CD34/kg. The target dose was achieved on Day 1 of collection in 19 collections (95%), with the target achieved on Day 2 in the single remaining patient. There was one incidence of hypocalcemia with no serious AEs. Average collection efficiency (CE)1 and CE2 were 59% and 49%.</p><p><strong>Discussion: </strong>HPC(A) is feasible and safe in patients ≤10 kg, including with ACD-A + heparin anticoagulation, supporting broader application in this population.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1640-1649"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The novel c.1227+2T>C variant in RHD results in the serological D-negative phenotype.","authors":"Jong Kwon Lee, Ja-Hyun Jang, Jiyoung Seo, Duck Cho, Jihyang Lim","doi":"10.1111/trf.18334","DOIUrl":"10.1111/trf.18334","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"E43-E45"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating RhD assessment by automated methodology: A potential \"blind spot\" for RhD variant identification.","authors":"Nalan Yurtsever, Christopher A Tormey, Laurie Bizzario, Edward S Lee","doi":"10.1111/trf.18343","DOIUrl":"10.1111/trf.18343","url":null,"abstract":"<p><strong>Background: </strong>The Rh blood group is highly polymorphic, and individuals with RHD variant alleles can form antibodies against antigens found in the conventional RhD protein. To prevent transfusion of D+ blood to groups at risk of forming anti-D antibodies, such as women of childbearing age and newborns, our transfusion service has established protocols to accurately identify D variants that can be missed by automated testing platforms in these important patient populations.</p><p><strong>Study design and methods: </strong>We implemented a blood bank protocol at Yale New Haven Hospital to identify patients to perform RHD genotyping on and evaluated the effectiveness of the protocol to detect D variants in patients undergoing ABO/D typing from December 2020 to January 2024. We compared serological reactivities between automated platforms (Grifols gel column and Werfen solid phase) and traditional tube testing on patients with confirmed RHD genotyping.</p><p><strong>Results: </strong>Among 74 patients genotyped, 53 exhibited D variants, yielding a positive predictive value (PPV) of 71.6%. A total of 29 patients had variant D types associated with anti-D formation. Tube testing showed significantly lower reactivity compared to the gel platform (p = .0001). Solid-phase testing did not demonstrate significant differences from tube testing (p = .15).</p><p><strong>Discussion: </strong>Our findings reveal a critical \"blind spot\" in automated gel platforms, which may lead to misclassification of D variants. Our established protocol effectively identifies high-risk patients who need RHD genotyping by using routine tube testing. This approach aims to minimize missed cases of clinically significant D variants, ultimately improving patient safety in transfusion practices.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":"65 9","pages":"1574-1579"},"PeriodicalIF":2.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145055908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}