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Novel RHD allele with c.487-2A>G (IVS3-2A>G) variant causing RhD negative phenotype. c.487-2A>G (IVS3-2A>G)变异的新型 RHD 等位基因导致 RhD 阴性表型。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-02 DOI: 10.1111/trf.17979

Yuli Zhu, Lihong Jiang, Zhihui Feng, Aiying Wang

引用次数: 0

Molecular communication between red blood cells of different phenotypes during blood storage in mice. 小鼠血液储存过程中不同表型红细胞之间的分子交流。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-02 DOI: 10.1111/trf.17960

James C Zimring, Ariel M Hay, Monika Dzieciatkowska, Angelo D'Alessandro

{"title":"Molecular communication between red blood cells of different phenotypes during blood storage in mice.","authors":"James C Zimring, Ariel M Hay, Monika Dzieciatkowska, Angelo D'Alessandro","doi":"10.1111/trf.17960","DOIUrl":"10.1111/trf.17960","url":null,"abstract":"Background: The cellular and molecular changes during red blood cell (RBC) storage that affect posttransfusion recovery (PTR) remain incompletely understood. We have previously reported that RBCs of different storage biology cross-regulate each other when stored together (co-storage cross-regulation [CSCR]). However, the mechanism of CSCR is unclear. In the current study, we tested the hypothesis that CSCR involves acquisition of molecular signatures associated with PTR.Study design and methods: The whole blood compartment of either B6 or FVB mice was biotinylated in vivo prior to blood collection and storage. Bio-B6 or Bio.FVB were stored with RBCs from B6 mice transgenic for green florescent protein (GFP) (B6.GFP). After storage, avidin-magnetic beads were used to simultaneous purify Bio-RBCs (positive selection) and B6.GFPs (negative selection). Isolated populations were analyzed by transfusion to establish PTR, and subjected to metabolomic and proteomic analysis.Results: B6 RBCs acquired molecular signatures associated with stored FVB RBCs at both the metabolomic and proteomic level including metabolites associated with energy metabolism, oxidative stress regulation, and oxidative damage. Mitochondrial signatures were also acquired by B6 RBCs. Protein signatures acquired by B6 RBCs include proteins associated with vesiculation.Conclusion: The data presented herein demonstrate the appearance of multiple molecular changes from poor-storing RBCs in good-storing RBCs during co-storage. Whether this is a result of damage causing intrinsic molecular changes in B6 RBCs or if molecules of FVB RBC origin are transferred to B6 RBCs remains unclear. These studies broaden our mechanistic understanding of RBC storage (in particular) and potentially RBC biology (in general).","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current state of undergraduate medical school training in transfusion medicine and its impact on postgraduate trainee knowledge. 医学院本科生输血医学培训现状及其对研究生学员知识的影响。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-06-12 DOI: 10.1111/trf.17918

Mahboubeh Rahmani, Sophie Chargé, Melanie Bodnar, Jeannie Callum, Cyrus Hsia, Marianne Lavoie, Anne-Sophie Lemay, Johnathan Mack, Oksana Prokopchuk-Gauk, Jacqueline Trudeau, Michelle P Zeller, Yulia Lin

{"title":"Current state of undergraduate medical school training in transfusion medicine and its impact on postgraduate trainee knowledge.","authors":"Mahboubeh Rahmani, Sophie Chargé, Melanie Bodnar, Jeannie Callum, Cyrus Hsia, Marianne Lavoie, Anne-Sophie Lemay, Johnathan Mack, Oksana Prokopchuk-Gauk, Jacqueline Trudeau, Michelle P Zeller, Yulia Lin","doi":"10.1111/trf.17918","DOIUrl":"10.1111/trf.17918","url":null,"abstract":"Background: Studies have described poor transfusion medicine (TM) knowledge in postgraduate trainees. The impact of undergraduate medical TM education on postgraduate knowledge is unclear.Methods: Canadian medical schools were surveyed on the number of hours dedicated to TM teaching and topics covered by curricula during 2016-2020. Postgraduate trainees attending Transfusion Camp in 2021 completed a pretest of 20 multiple-choice questions. The survey results and pretest scores were compared to evaluate the association between undergraduate medical TM education and pretest scores.Results: The survey was completed by 16 of 17 Canadian medical schools. The number of hours (h) of TM teaching were <2 h (25%), 3-4 h (25%), and >4 h (50%). Twelve of 19 Transfusion Camp topics were covered in ≥50% of schools. Eleven medical schools provided ethics approvals/waivers to include trainee pretest scores in the analysis (N = 200). The median pretest scores by medical school ranged from 48% to 70%. No association was found between number of TM teaching hours and average pretest scores (p = .60). There was an association between higher postgraduate year level and individual pretest score (p < .0001). The analysis by topic demonstrated questions where trainees from different schools performed uniformly well or poorly; other topics showed considerable variation.Conclusion: Variation in quantity and content of undergraduate TM teaching exists across Canadian medical schools. In this limited assessment, the number of TM teaching hours was not associated with performance on the pretest. This study raises the opportunity to re-evaluate the delivery (content, timing, consistency) of TM education in undergraduate medical schools.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consensus transfusion guidelines for a large neonatal intensive care network. 大型新生儿重症监护网络的一致输血指南。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1111/trf.17914

Lindsay E Gilmore, Stella T Chou, Sarvin Ghavam, Christopher S Thom

引用次数: 0

Incidentally detected West Nile virus-RNA positive blood donors do not harbor high titers of neutralizing antibodies. 偶然检测到的西尼罗河病毒-RNA 阳性献血者体内并不存在高滴度的中和抗体。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 DOI: 10.1111/trf.17946

Silvia Meschi, Francesca Colavita, Giulia Matusali, Daniele Focosi, Fabrizio Maggi, Massimo Franchini

引用次数: 0

Current advances in 2024: A critical review of selected topics by the Association for the Advancement of Blood and Biotherapies (AABB) Clinical Transfusion Medicine Committee. 2024 年的最新进展：血液与生物治疗促进协会（AABB）临床输血医学委员会对选定主题的严格审查。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 DOI: 10.1111/trf.17975

Jacqueline N Poston, Jennifer Andrews, Sumedha Arya, Stella T Chou, Claudia Cohn, Mischa Covington, Elizabeth P Crowe, Ruchika Goel, Gaurav K Gupta, Richard L Haspel, Aaron Hess, Tina S Ipe, Jessica Jacobson, Jenna Khan, Mike Murphy, Kerry O'Brien, Monica B Pagano, Anil K Panigrahi, Eric Salazar, Nabiha H Saifee, Moritz Stolla, Nicole D Zantek, Alyssa Ziman, Ryan A Metcalf

引用次数: 0

Impact of a single blood donation on hemoglobin mass, iron stores, and maximum oxygen uptake in pre-menopausal women-A pilot study. 单次献血对绝经前妇女血红蛋白质量、铁储存和最大摄氧量的影响--一项试点研究。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1111/trf.17917

Nadine B Wachsmuth, Sandra Haupt, Philipp Bauer, Janis R Schierbauer, Gunnar Treff, Jürgen M Steinacker, Sian Rilstone, Walter F J Schmidt

{"title":"Impact of a single blood donation on hemoglobin mass, iron stores, and maximum oxygen uptake in pre-menopausal women-A pilot study.","authors":"Nadine B Wachsmuth, Sandra Haupt, Philipp Bauer, Janis R Schierbauer, Gunnar Treff, Jürgen M Steinacker, Sian Rilstone, Walter F J Schmidt","doi":"10.1111/trf.17917","DOIUrl":"10.1111/trf.17917","url":null,"abstract":"Background: During whole blood donation (BD), 500 mL of blood is drawn. The time interval between two BDs is at least 8-12 weeks. This period might be insufficient for restoring hemoglobin mass (Hbmass) and iron especially in women, who generally have lower Hbmass and iron availability. Since both variables influence physical performance, this pilot study aimed to monitor Hbmass, iron status, and maximum oxygen uptake (V̇O2max) recovery in women after a single BD.Study design and methods: In 10 women (24.7 ± 1.7 years), Hbmass, hemoglobin concentration [Hb], iron status, and V̇O2max were assessed before and up to 12 weeks after a single BD.Results: BD reduced Hbmass from 562 ± 70 g to 499 ± 64 g (p < .001). Although after 8 weeks no significant mean difference was detected, 7 women had not returned to baseline after 12 weeks. [Hb] did not return to initial values (13.4 ± 0.7 g/dL) after 12 weeks (12.9 ± 0.7 g/dL, p < .01). Ferritin decreased from baseline until week 6 (40.9 ± 34.2 ng/mL vs. 12.1 ± 6.9 ng/mL, p < .05) and was not restored after 12 weeks (18.4 ± 12.7 ng/mL, p < .05), with 6 out of 10 women exhibiting iron deficiency (ferritin <15 ng/mL). V̇O2max was reduced by 213 ± 47 mL/min (7.2 ± 1.2%; p < .001) and remained below baseline after 12 weeks (3.2 ± 1.4%, p < .01).Discussion: For most pre-menopausal women, 12 weeks were not sufficient to recover from BD and achieve baseline Hbmass and iron stores resulting in prolonged reduction of aerobic capacity. A subsequent BD might lead to a severe anemia.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bleeding assessment following central venous catheter placement, a direct comparison of prospective and retrospective analyses. 置入中心静脉导管后的出血评估，前瞻性分析与回顾性分析的直接比较。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-06-26 DOI: 10.1111/trf.17930

Floor L F van Baarle, Emma K van de Weerdt, S Jorinde Raasveld, Alexander P J Vlaar, Bart J Biemond

{"title":"Bleeding assessment following central venous catheter placement, a direct comparison of prospective and retrospective analyses.","authors":"Floor L F van Baarle, Emma K van de Weerdt, S Jorinde Raasveld, Alexander P J Vlaar, Bart J Biemond","doi":"10.1111/trf.17930","DOIUrl":"10.1111/trf.17930","url":null,"abstract":"Background: Reported bleeding incidences following central venous catheter (CVC) placement highly depend on methods of bleeding assessment. To determine the direction and magnitude of the bias associated with retrospective data collection, we used data from the PACER randomized controlled trial and a previous retrospective cohort study.Study design and methods: A patient-level comparison of CVC-related bleeding severity was made among (1) the prospectively collected clinical bleeding assessment of the PACER trial, (2) centralized assessment of CVC insertion site photographs, and (3) retrospective chart review. Interrater reliability for photographic bleeding assessment and retrospective chart review was assessed using Cohen's κ. The magnitude of underreporting of both methods compared to prospective clinical bleeding assessment at different cutoff points of clinically relevant bleeding was assessed using McNemar's test.Results: Interrater reliability was acceptable for both methods (κ = 0.583 and κ = 0.481 for photographic assessment and retrospective chart review, respectively). Photographic bleeding assessment led to significant underreporting of bleeding complications at all cutoff points. Retrospective chart review led to significant underreporting of minor bleeding complications, with an odds ratio (95% CI) of 0.17 (0.044-0.51) for the cutoff point grade 1 (i.e., self-limiting or requiring at most 20 min of manual compression) or higher. There was no significant underreporting of major bleeding complications with retrospective chart review.Discussion: Centralized photographic bleeding assessment and retrospective chart review lead to biased bleeding assessment compared to prospective clinical bleeding assessment.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141459478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

To exchange or not exchange: That is the question. 交换还是不交换？这就是问题所在。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-07-14 DOI: 10.1111/trf.17958

Patricia A Shi

引用次数: 0

Testosterone supplementation increases red blood cell susceptibility to oxidative stress, decreases membrane deformability, and decreases survival after cold storage and transfusion. 补充睾酮会增加红细胞对氧化应激的敏感性，降低膜的变形能力，并降低冷藏和输血后的存活率。

IF 2.5 3区医学

Transfusion Pub Date : 2024-08-01 Epub Date: 2024-06-17 DOI: 10.1111/trf.17922

Johnson Tran, Rachael P Jackman, Marcus O Muench, Kelsey Hazegh, Scott-Wesley Bean, Kimberly A Thomas, Fang Fang, Grier Page, Kim O'Connor, Nareg H Roubinian, Bradley D Anawalt, Tamir Kanias

{"title":"Testosterone supplementation increases red blood cell susceptibility to oxidative stress, decreases membrane deformability, and decreases survival after cold storage and transfusion.","authors":"Johnson Tran, Rachael P Jackman, Marcus O Muench, Kelsey Hazegh, Scott-Wesley Bean, Kimberly A Thomas, Fang Fang, Grier Page, Kim O'Connor, Nareg H Roubinian, Bradley D Anawalt, Tamir Kanias","doi":"10.1111/trf.17922","DOIUrl":"10.1111/trf.17922","url":null,"abstract":"Background: Blood collection from donors on testosterone therapy (TT) is restricted to red blood cell (RBC) concentrates to avoid patient exposure to supraphysiological testosterone (T). The objective of this study was to identify TT-related changes in RBC characteristics relevant to transfusion effectiveness in patients.Study design: This was a two-part study with cohorts of patients and blood donors on TT. In part 1, we conducted longitudinal evaluation of RBCs collected before and at three time points after initiation of T. RBC assays included storage and oxidative hemolysis, membrane deformability (elongation index), and oximetry. In part 2, we evaluated the fate of transfused RBCs from TT donors in immunodeficient mice and by retrospective analyses of NIH's vein-to-vein databases.Results: TT increased oxidative hemolysis (1.45-fold change) and decreased RBC membrane deformability. Plasma free testosterone was positively correlated with oxidative hemolysis (r = .552) and negatively correlated with the elongation index (r = -.472). Stored and gamma-irradiated RBCs from TT donors had lower posttransfusion recovery in mice compared to controls (41.6 ± 12 vs. 55.3 ± 20.5%). Recipients of RBCs from male donors taking T had 25% lower hemoglobin increments compared to recipients of RBCs from non-TT male donors, and had increased incidence (OR, 1.80) of requiring additional RBC transfusions within 48 h of the index transfusion event.Conclusions: TT is associated with altered RBC characteristics and transfusion effectiveness. These results suggest that clinical utilization of TT RBCs may be less effective in recipients who benefit from longer RBC survival, such as chronically transfused patients.","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11316632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0