Transgenic Research最新文献

{"title":"Overexpression of NAC transcription factors from Eremopyrum triticeum promoted abiotic stress tolerance.","authors":"Xue-Ni Zhong, Jun-Jie Peng, Meng-Yao Wang, Xiu-Li Yang, Li Sun","doi":"10.1007/s11248-024-00428-3","DOIUrl":"https://doi.org/10.1007/s11248-024-00428-3","url":null,"abstract":"<p><p>Eremopyrum triticeum is a typical spring ephemeral species, which in China mainly distributed in the desert regions of northern Xinjiang, and play an important role in the desert ecosystems. E. triticeum has several adaptive characteristics such as short growth rhythms, high photosynthetic efficiency, high seed production, drought and salt resistance. However, the molecular regulatory mechanism of E. triticeum in responses to abiotic stress resistance is still unknown. In this study, two NAC-like transcription factor-encoding genes, EtNAC1 and EtNAC2, were isolated from E. triticeum. The predicted EtNAC1 and EtNAC2 proteins possess a typical NAC DNA-binding domain at the N-terminal region. The qRT-PCR analysis showed that EtNAC1 and EtNAC2 were highly expressed in mature roots of E. triticeum, and were significantly up-regulated under drought, high salt and abscisic acid (ABA) stresses. Subcellular localization analysis in onion epidermal cells revealed that EtNAC1 and EtNAC2 were located in the nucleus. Expression of EtNAC1 and EtNAC2 in yeast cells improved the survival rate of yeast under low temperature, H₂O₂, high drought and salt stresses. Overexpression of EtNAC1 and EtNAC2 in Arabidopsis thaliana conferred enhanced tolerance to drought and salt stresses, increased ABA sensitivity, and transgenic plants showed higher proline (Pro) content, but lower malondialdehyde content, lower chlorophyll leaching, lower water loss rate and stomatal aperture (width/length) than WT plants. In conclusion, EtNAC1 and EtNAC2 play important roles in abiotic stress responses of E. triticeum, which might have significant potential in crop molecular breeding for abiotic stress tolerance.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"3"},"PeriodicalIF":2.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay between Agrobacterium T-DNA and backbone DNA in transgenic plant cells.","authors":"Peng Xu, Shaojuan Lai, Bo Yin, Chenyu Yao, Xiaolin Gu, Jilei Huang, Yufei Hu","doi":"10.1007/s11248-024-00424-7","DOIUrl":"https://doi.org/10.1007/s11248-024-00424-7","url":null,"abstract":"<p><p>Agrobacterium-mediated transformation of plants often results in the integration of multiple copies of T-DNA and backbone DNA from binary vectors into the host genome. However, the interplay between T-DNA and backbone DNA remains elusive. In this study, 70.8% of T₁ Arabidopsis transformants exhibited integration of both T-DNA and backbone DNA, and no cases of only backbone integration were observed. To elucidate the integration patterns, we employed bulk-genome resequencing in Arabidopsis and identified 20 integration sites across 10 T₁ transgenic plants, most of which were flanked by left borders of T-DNA at both ends. On average, each integration site contained 6.3 copies of T-DNA and 2.65 copies of backbone DNA. The junction structures between T-DNA and the backbone were highly variable, revealing a previously underappreciated frequency of readthrough at both the left and right borders. Transient expression studies in Nicotiana benthamiana leaves demonstrated that T-DNA and backbone DNA were simultaneously transferred into transformed cells, although the backbone DNA had lower copy numbers than T-DNA. These findings suggest a close relationship between T-DNA and backbone DNA during their transfer and integration, thus offering new insights into the mechanism underlying Agrobacterium-mediated transformation.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"1"},"PeriodicalIF":2.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainability requires the integration of farmer knowledge, scientific advancements, and comprehensive innovation.","authors":"Urs Niggli","doi":"10.1007/s11248-024-00414-9","DOIUrl":"10.1007/s11248-024-00414-9","url":null,"abstract":"<p><p>This viewpoint paper emphasises the need to diversify food production methods to simultaneously combat hunger and reduce environmental problems. The recommendations of the UN Food System Summit 2021 relate primarily to (i) the conservation of natural ecosystems, (ii) the sustainable management of existing agricultural land while increasing productivity and (iii) the restoration of already degraded land. Europe in particular faces unique challenges, such as reducing pollution and promoting organic farming up to 25 percent of the agricultural land area while maintaining food production. Ongoing efforts aim to create a transparent, fair and multi-level regulatory framework to support the Green Deal. The implementation of the Corporate Sustainability Reporting Directive (CSRD), which will sooner or later affect a larger proportion of European farmers, should support the transition. Science and innovation play a central role in this, as they are the cornerstones on which sustainable food systems are built. It is imperative that farmers actively participate in the co-design processes and utilise their wealth of experience and creativity to drive these innovations forward. A crucial aspect of the transition to sustainability is changing consumption patterns to limit food waste and reduce meat consumption. While this transition is essential, it is not without its formidable challenges. Diversification of agriculture, encompassing a spectrum of established techniques, is touted as a promising approach to achieving sustainability without sacrificing productivity. Furthermore, integrating truly sustainable agricultural practices with cutting-edge innovations, including new genomic techniques, has the potential to be a transformative solution.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"563-569"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global conference on sustainability in agriculture & food systems: innovation, indicators and implementation.","authors":"Peter Kearns, Steffi Friedrichs","doi":"10.1007/s11248-024-00418-5","DOIUrl":"10.1007/s11248-024-00418-5","url":null,"abstract":"","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"537-538"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142839856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision breeding in agriculture and food systems in the United Kingdom.","authors":"Oli Watson, Sadiye Hayta","doi":"10.1007/s11248-024-00397-7","DOIUrl":"10.1007/s11248-024-00397-7","url":null,"abstract":"<p><p>In recent years there have been major advances in precision breeding technologies, such as gene editing, that offer promising solutions to revolutionise global crop production and tackle the pressing issues in food systems. The UK has leading expertise in genomics, and research is already taking place to develop crops with improved resilience to climate change, resistance to disease and less reliance on chemical inputs. In March 2023, the Genetic Technology (Precision Breeding) Act received Royal Assent and passed into UK law. It provides a framework from which to build more proportionate regulations for plants and animals made using genetic technologies which contain genetic changes that could also arise through traditional breeding-known as 'Precision Bred Organisms'. New legislation and the utilization of UK world-leading research could help to enhance the efficiency of breeding systems and enable the development of plants and animals that are healthier, better for the environment and more resilient to climate change.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"539-544"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limitations in measuring sustainable food systems: domestic policy priorities and global monitoring.","authors":"Ritsuko Yoneda","doi":"10.1007/s11248-024-00417-6","DOIUrl":"10.1007/s11248-024-00417-6","url":null,"abstract":"<p><p>In recent years, many strategies for sustainable food systems have been launched at the national and global levels, which require better tools to monitor their progress. Subsequently, discussions on their measurements have drawn enormous attention, and various indicators have been developed. As indicators at the national level reflect policy priorities in the respective countries, it is difficult to develop adequate global indicators that accommodate different national priorities. Additionally, if we pursue only the existing dataset, we may lose the thrust of the initial objectives. However, the collection of new data can place an enormous burden on stakeholders, both developing and developed countries. These difficulties were revealed in recent negotiations for the Kunming-Montreal Global Biodiversity Framework at the Convention on Biological Diversity at the 15th Conference of the Parties. Therefore, we must reach a compromise between what we want to achieve and the resources we can share.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"571-575"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Narratives in European debate concerning new genomic techniques.","authors":"Marcin Napiórkowski, Andrzej Nowak, Mikołaj Biesaga, Szymon Talaga, Erika Staël von Holstein","doi":"10.1007/s11248-024-00416-7","DOIUrl":"10.1007/s11248-024-00416-7","url":null,"abstract":"<p><p>Given the complexity of agricultural problems, it is essential to develop acceptable solutions for various stakeholders with diverse knowledge, viewpoints, and preferences. However, European public opinion has become highly polarized, making constructive discussions on these issues difficult. We present the results of the narrative analysis of media debate on new genomic techniques. The study identified two primary narrative groups: 'precaution-focused' and 'innovation-focused.' The former emphasizes caution, potential risks, and the need for stringent regulation, while the latter highlights benefits, progress, and the promise of genome editing for sustainable agricultural practices. Within each group of narratives, several distinct narratives were identified. The research has revealed that despite the high polarization, the narratives shared important values and beliefs. Going beyond the dividing narratives and concentrating on common values can depolarize the debate and set the stage for new narratives, enabling constructive debate, concentrating on solving problems, and maximizing collective outcomes.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"551-561"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of genome edited organisms in Australia.","authors":"Peter Thygesen","doi":"10.1007/s11248-024-00411-y","DOIUrl":"10.1007/s11248-024-00411-y","url":null,"abstract":"<p><p>Whether organisms developed with the use of genome editing techniques, or food derived from such organisms, are, or should be, regulated as genetically modified organisms (GMOs) or genetically modified (GM) food, respectively, remains a subject of debate globally. Much of the discussion has been scientific and focussed on the similar genetic outcomes of some genome editing techniques and 'conventional' or natural mutagenesis. Many jurisdictions, including Australia, have considered, or are considering, how their regulatory frameworks will deal with such organisms and products. In Australia, organisms developed with site directed nuclease 1 (SDN-1, with no added template to guide homology-directed repair) are not regulated as GMOs, pursuant to exclusions in the Gene Technology Regulations 2001. The exclusion of SDN-1 organisms from regulation in Australia is sometimes misrepresented, including in scientific peer reviewed publications, as extending to all genome edited organisms. This highlights the importance for researchers, developers and other stakeholders to understand that whether genome edited organisms are, or are not, subject to regulation as GMOs in a particular jurisdiction may quintessentially be a legal question, not a scientific one.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"545-550"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: WIF1 causes dysfunction of heart in transgenic mice.","authors":"Dan Lu, Wei Dong, Xu Zhang, Xiongzhi Quan, Dan Bao, Yingdong Lu, Lianfeng Zhang","doi":"10.1007/s11248-024-00403-y","DOIUrl":"10.1007/s11248-024-00403-y","url":null,"abstract":"","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"535-536"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141983369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing a lethal CAG-ACE2 transgenic mouse model for SARS-CoV-2 infection using Cas9-enhanced nanopore sequencing.","authors":"Alexander Smirnov, Artem Nurislamov, Galina Koncevaya, Irina Serova, Evelyn Kabirova, Eduard Chuyko, Ekaterina Maltceva, Maxim Savoskin, Daniil Zadorozhny, Victor A Svyatchenko, Elena V Protopopova, Oleg S Taranov, Stanislav S Legostaev, Valery B Loktev, Oleg Serov, Nariman Battulin","doi":"10.1007/s11248-024-00413-w","DOIUrl":"10.1007/s11248-024-00413-w","url":null,"abstract":"<p><p>The SARS-CoV-2 pandemic has underscored the necessity for functional transgenic animal models for testing. Mouse lines with overexpression of the human receptor ACE2 serve as the common animal model to study COVID-19 infection. Overexpression of ACE2 under a strong ubiquitous promoter facilitates convenient and sensitive testing of COVID-19 pathology. We performed pronuclear microinjections using a 5 kb CAG-ACE2 linear transgene construct and identified three founder lines with 140, 72, and 73 copies, respectively. Two of these lines were further analyzed for ACE2 expression profiles and sensitivity to SARS-CoV-2 infection. Both lines expressed ACE2 in all organs analyzed. Embryonic fibroblast cell lines derived from transgenic embryos demonstrated severe cytopathic effects following infection, even at low doses of SARS-CoV-2 (0,1-1.0 TCID₅₀). Infected mice from the two lines began to show COVID-19 clinical signs three days post-infection and succumbed between days 4 and 7. Histological examination of lung tissues from terminally ill mice revealed severe pathological alterations. To further characterize the integration site in one of the lines, we applied nanopore sequencing combined with Cas9 enrichment to examine the internal transgene concatemer structure. Oxford Nanopore sequencing (ONT) is becoming the gold standard for transgene insert characterization, but it is relatively inefficient without targeted region enrichment. We digested genomic DNA with Cas9 and gRNA against the ACE2 transgene to create ends suitable for ONT adapter ligation. ONT data analysis revealed that most of the transgene copies were arranged in a head-to-tail configuration, with palindromic junctions being rare. We also detected occasional plasmid backbone fragments within the concatemer, likely co-purified during transgene gel extraction, which is a common occurrence in pronuclear microinjections.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"453-466"},"PeriodicalIF":2.7,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}