Transgenic Research最新文献_第2页

CRISPR/Cas12a ribonucleoprotein mediated editing of tryptophan 2,3-dioxygenase of Spodoptera frugiperda. CRISPR/Cas12a核糖核蛋白介导的弗氏蝶类色氨酸 2,3-二氧化酶编辑。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-29 DOI: 10.1007/s11248-024-00406-9

Dhawane Yogi, Karuppannasamy Ashok, Cholenahalli Narayanappa Anu, Thalooru Shashikala, Chalapathy Pradeep, Chikmagalur Nagaraja Bhargava, Madhusoodanan Sujatha Parvathy, M N Jithesh, Maligeppagol Manamohan, Girish Kumar Jha, Ramasamy Asokan

{"title":"CRISPR/Cas12a ribonucleoprotein mediated editing of tryptophan 2,3-dioxygenase of Spodoptera frugiperda.","authors":"Dhawane Yogi, Karuppannasamy Ashok, Cholenahalli Narayanappa Anu, Thalooru Shashikala, Chalapathy Pradeep, Chikmagalur Nagaraja Bhargava, Madhusoodanan Sujatha Parvathy, M N Jithesh, Maligeppagol Manamohan, Girish Kumar Jha, Ramasamy Asokan","doi":"10.1007/s11248-024-00406-9","DOIUrl":"https://doi.org/10.1007/s11248-024-00406-9","url":null,"abstract":"In insect genome editing CRISPR/Cas9 is predominantly employed, while the potential of several classes of Cas enzymes such as Cas12a largely remain untested. As opposed to Cas9 which requires a GC-rich protospacer adjacent motif (PAM), Cas12a requires a T-rich PAM and causes staggered cleavage in the target DNA, opening possibilities for multiplexing. In this regard, the utility of Cas12a has been shown in only a few insect species such as fruit flies and the silkworm, but not in non-model insects such as the fall armyworm, Spodoptera frugiperda, a globally important invasive pest that defies most of the current management methods. In this regard, a more recent genetic biocontrol method known as the precision-guided sterile insect technique (pgSIT) has shown successful implementation in Drosophila melanogaster, with certain thematic adaptations required for application in agricultural pests. However, before the development of a controllable gene drive for a non-model species, it is important to validate the activity of Cas12a in that species. In the current study we have, for the first time, demonstrated the potential of Cas12a by editing an eye color gene, tryptophan 2,3-dioxygenase (TO) of S. frugiperda by microinjecting ribonucleoprotein complex into pre-blastoderm (G0) eggs. Analysis of G0 mutants revealed that all five mutants (two male and three female) exhibited distinct edits consisting of both deletion and insertion events. All five edits were further validated through in silico modeling to understand the changes at the protein level and further corroborate with the range of eye-color phenotypes observed in the present study.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term (10-year) monitoring of transposon-mediated transgenic cattle. 对转座子介导的转基因牛进行长期（10 年）监测。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-28 DOI: 10.1007/s11248-024-00401-0

Soo-Young Yum, Bae Young Choi, Gyeong-Min Gim, Kyeong-Hyeon Eom, Seong-Beom Lee, Daehyun Kim, Euntaek Lim, Do-Yoon Kim, Seong-Eun Heo, Donghwan Shim, Goo Jang

{"title":"Long-term (10-year) monitoring of transposon-mediated transgenic cattle.","authors":"Soo-Young Yum, Bae Young Choi, Gyeong-Min Gim, Kyeong-Hyeon Eom, Seong-Beom Lee, Daehyun Kim, Euntaek Lim, Do-Yoon Kim, Seong-Eun Heo, Donghwan Shim, Goo Jang","doi":"10.1007/s11248-024-00401-0","DOIUrl":"https://doi.org/10.1007/s11248-024-00401-0","url":null,"abstract":"The production of transgenic animals using non-viral methods has raised questions regarding their long-term health and genomic stability. In this study, we evaluated these aspects in transgenic cattle over ten years, using transposon-mediated gene transfer. Our longitudinal analysis included a comprehensive health assessment and whole-genome DNA resequencing. We found no significant alterations in physiological parameters or health complications in transposon-mediated transgenic cattle that exceeded 10 years of age. Genomic analysis revealed that the rates of somatic mutations and copy number variations in transgenic cattle were comparable to those in non-transgenic cattle. Furthermore, structural variants were infrequent, suggesting that transposon-mediated gene insertion did not compromise genomic integrity. These findings highlight the viability of transposon systems for generating transgenic livestock, potentially expanding their applications in agriculture and biotechnology. This study contributes significantly to our understanding of the long-term implications of transgenesis in large animals and supports the safety and stability of this method.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142081705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mosquito pollination of plants: an overview of their role and an assessment of the possible contribution of disease vectors. 蚊子为植物授粉：概述其作用并评估病媒的可能贡献。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-22 DOI: 10.1007/s11248-024-00394-w

Woodbridge A Foster

{"title":"Mosquito pollination of plants: an overview of their role and an assessment of the possible contribution of disease vectors.","authors":"Woodbridge A Foster","doi":"10.1007/s11248-024-00394-w","DOIUrl":"https://doi.org/10.1007/s11248-024-00394-w","url":null,"abstract":"Mosquitoes visit flowers to obtain sugar or other nutrients and therefore possibly serve as major or minor pollinators of some plant species. They also often derive plant nutrients from other sources, such as extrafloral nectaries and honeydew. In a few cases, the plant-mosquito relationship is close, and mosquito pollination has been confirmed. Most plant species visited by mosquitoes, however, appear to depend on multiple means of pollination, particularly other flower-feeding insects. In addition, most mosquito species visit the flowers of many kinds of plants, possibly dispersing pollen in both biologically meaningful and irrelevant ways. This apparent lack of selectivity by both plants and mosquitoes liberates each of them from dependence on an unreliable pollen vehicle or nutrient source. A hypothetical pollinating role for the two top vectors of devastating human-disease pathogens, Anopheles gambiae or Aedes aegypti, relies on indirect evidence. So far, this evidence suggests that their participation in pollen transfer of native, introduced, or beneficial plants is negligible. The few plant species likely to be pollinated by these vectors are mostly invasive, harmful weeds associated with humans. That conclusion draws support from four characteristics of these vectors: (1) the numerous alternative potential pollinators of the flowers they visit; (2) their common use of diverse non-floral sources of nutrients; (3) the females' infrequent sugar feeding and heavy reliance on human blood for energy; and (4) their relatively low population densities. From these traits it follows that focused suppression or elimination of these two vectors, by whatever means, is highly unlikely to have adverse effects on pollination in endemic biotic communities or on ornamental plants or food crops.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142018707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-generation CRISPR technology for genome, epigenome and mitochondrial editing. 用于基因组、表观基因组和线粒体编辑的新一代 CRISPR 技术。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-19 DOI: 10.1007/s11248-024-00404-x

Cia-Hin Lau, Qing-Le Liang, Haibao Zhu

引用次数: 0

Correction to: WIF1 causes dysfunction of heart in transgenic mice. 更正：WIF1 导致转基因小鼠心脏功能障碍。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-15 DOI: 10.1007/s11248-024-00403-y

Dan Lu, Wei Dong, Xu Zhang, Xiongzhi Quan, Dan Bao, Yingdong Lu, Lianfeng Zhang

引用次数: 0

Functional analysis of root-preferential oil palm metallothionein promoter in tobacco. 烟草根系偏好油棕金属硫蛋白启动子的功能分析

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-09 DOI: 10.1007/s11248-024-00396-8

Subhi Siti Masura, Noor Azmi Shaharuddin, Mat Yunus Abdul Masani, Kuang-Lim Chan, Eng-Ti Leslie Low, Pek-Lan Chan, Abdul Rahman Siti Rahmah, Nadzirah Amiruddin, Mohd Puad Abdullah, Azzreena Mohamad Azzeme, Ghulam Kadir Ahmad Parveez, Omar Abd Rasid

{"title":"Functional analysis of root-preferential oil palm metallothionein promoter in tobacco.","authors":"Subhi Siti Masura, Noor Azmi Shaharuddin, Mat Yunus Abdul Masani, Kuang-Lim Chan, Eng-Ti Leslie Low, Pek-Lan Chan, Abdul Rahman Siti Rahmah, Nadzirah Amiruddin, Mohd Puad Abdullah, Azzreena Mohamad Azzeme, Ghulam Kadir Ahmad Parveez, Omar Abd Rasid","doi":"10.1007/s11248-024-00396-8","DOIUrl":"https://doi.org/10.1007/s11248-024-00396-8","url":null,"abstract":"Root-specific or preferential promoters are essential to genetically modify plants with beneficial root traits. We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expression in roots. Stable functional characterisation of tobacco transgenic lines using the T1 generation showed that a deletion construct, designated as RSP-2D (1107 bp), directed strong GUS expression at all stages of root development, particularly in mature roots. Other constructs, RSP-2A (2481 bp) and RSP-2C (1639 bp), drove GUS expression in roots with an intensity lower than RSP-2D. The promoter activity was also detectable in seed pods and immature seeds, albeit at lower levels than CaMV35S. The promoter activity may also be induced by wounding as intact GUS staining was observed at the flower- and leaf-cutting sites of T1 samples carrying either RSP-2C or RSP-2D constructs. The promoter sequence contains cis-acting elements that may act as negative regulators and be responsible for root specificity. The results further indicated that the 5' UTR and ATATT sequences are essential for strong promoter activity. This study highlights the potential of RSP-2D promoter as a tool for modifying root traits through genetic engineering.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Mouse Model for Conditional Expression of Activated β-Catenin in Epidermal Keratinocytes. 表皮角质形成细胞中活化β-Catenin条件表达的小鼠模型

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-07 DOI: 10.1007/s11248-024-00402-z

Vineet K Maurya, Yan Ying, John P Lydon

{"title":"A Mouse Model for Conditional Expression of Activated β-Catenin in Epidermal Keratinocytes.","authors":"Vineet K Maurya, Yan Ying, John P Lydon","doi":"10.1007/s11248-024-00402-z","DOIUrl":"https://doi.org/10.1007/s11248-024-00402-z","url":null,"abstract":"We report the generation and characterization of the K5: CAT bigenic mouse in which the constitutively activated form of β-catenin (ΔN89 β-catenin) is conditionally expressed in cytokeratin-5 (K5) positive epidermal keratinocytes. Following short-term doxycycline intake during the telogen resting phase, the adult K5: CAT bigenic develops enlarged pilosebaceous units that expand deep into the dermis, an expansion usually observed during the anagen growth phase. Prolonged doxycycline treatment results in significant thickening and folding of the K5: CAT epidermis. During this persistent induction period, there is clear evidence of increased keratinocyte proliferation, particularly in the epidermal basal cell layer and the outer root sheath of the hair follicle. This unscheduled increase in cellular proliferation likely explains the decrease in hair density observed in the K5: CAT mouse following persistent doxycycline intake. Numerous hyperplastic endometrioid cysts, which display cornification toward their lumens, are also observed during this treatment period. Remarkably, de-induction of ΔN89 β-catenin expression through doxycycline withdrawal results in a marked reversal of the skin phenotype, suggesting that these morphological changes are dependent on continued signaling by β-catenin and/or its downstream molecular mediators. Joining a small group of mouse models for conditional β-catenin signaling, our K5: CAT mouse model will be particularly useful in identifying those molecular mediators of β-catenin that are responsible for initiating and maintaining these phenotypic responses in the K5: CAT skin. Such studies are predicted to shed more light on β-catenin signaling in epidermal epithelial morphogenesis, hair follicle cycling, and hair growth pathologies.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141898312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision breeding in agriculture and food systems in the United Kingdom. 英国农业和食品系统中的精准育种。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-06 DOI: 10.1007/s11248-024-00397-7

Oli Watson, Sadiye Hayta

引用次数: 0

Public perception of folate-biofortified genetically modified lettuce varieties in Brazil. 巴西公众对叶酸生物强化转基因莴苣品种的看法。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-05 DOI: 10.1007/s11248-024-00400-1

Thaís de Moura Cipriano, Maria Thereza Macedo Pedroso, Isis Amanda de Paula Nunes, Lídia Nascimento Queiroz, Francisco José Lima Aragão

{"title":"Public perception of folate-biofortified genetically modified lettuce varieties in Brazil.","authors":"Thaís de Moura Cipriano, Maria Thereza Macedo Pedroso, Isis Amanda de Paula Nunes, Lídia Nascimento Queiroz, Francisco José Lima Aragão","doi":"10.1007/s11248-024-00400-1","DOIUrl":"https://doi.org/10.1007/s11248-024-00400-1","url":null,"abstract":"Lettuce is one of the most widely consumed vegetables in the world, commonly eaten fresh in salads, sandwiches, wraps, and as a garnish in various dishes. Consequently, it is a very promising vehicle to deliver vitamins, such as folate (vitamin B9), to a specific population using biofortified varieties generated by conventional or molecular breeding. A new genetically modified lettuce was generated with increased folate content. However, some issues related to public perception regarding this technology should still be evaluated. The aim of this study was to analyze whether consumers are willing to accept a folate-biofortified GM lettuce that could become available to the Brazilian market. A questionnaire involving several issues regarding lettuce consumption was answered by 2,391 people from almost all Brazilian states. When informed that the folic acid biofortified lettuce is a transgenic plant, 46.1% of respondents stated that they would eat it and 30.5% stated that it would be a possibility. This study demonstrated that if there is any explanation regarding the advantage in relation to the use of biotechnology, like enrichment with folic acid, the number of people who accept it increases.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141894339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of a single-chain monellin (MNEI) mutant with enhanced stability in transgenic mice milk. 在转基因小鼠乳汁中表达稳定性增强的单链蒙奈林（MNEI）突变体。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-08-01 Epub Date: 2024-06-11 DOI: 10.1007/s11248-024-00389-7

Rui Lu, Xiaoming Li, Jian Hu, Yancui Wang, Le Jin

引用次数: 0