Transgenic Research最新文献_第2页

Transgenic overexpression of UDP glycosyltransferase gene UGT41A3 induces resistance to nucleopolyhedrovirus in Bombyx mori. UDP糖基转移酶基因UGT41A3的转基因过表达诱导家蚕对核多角体病毒的抗性

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-30 DOI: 10.1007/s11248-024-00422-9

Chunying Gong, Junwen Ai, Yong Liu, Xingjian He, Hong Xue, Chaohua Jia, Zhuohua Chen, Hanfu Xu, Rongpeng Liu, Yong Yang

{"title":"Transgenic overexpression of UDP glycosyltransferase gene UGT41A3 induces resistance to nucleopolyhedrovirus in Bombyx mori.","authors":"Chunying Gong, Junwen Ai, Yong Liu, Xingjian He, Hong Xue, Chaohua Jia, Zhuohua Chen, Hanfu Xu, Rongpeng Liu, Yong Yang","doi":"10.1007/s11248-024-00422-9","DOIUrl":"https://doi.org/10.1007/s11248-024-00422-9","url":null,"abstract":"Bombyx mori nuclear polyhedrosis, caused by B. mori nucleopolyhedrovirus (BmNPV), threatens sericulture seriously. To explore strategies for controlling it, the UDP glycosyltransferase gene UGT41A3 (BmUGT41A3) was targeted. UGT is involved in exogenous substances detoxification and endogenous biomass regulation in insects. Early embryos of the BmNPV-sensitive variety 'HYB' were used to obtain the transgenic line HYB-UGT41A3, overexpressing BmUGT41A3 under the IE1 promoter. qPCR results revealed that, compared with the wild-type control 'HYB', BmUGT41A3 was upregulated during the individual developmental stages of HYB-UGT41A3 from silkworm eggs to fifth-instar larvae; peak expression was observed in the third-instar larvae, which presented the most significantly upregulated expression. Individual-tissues qPCR results revealed that BmUGT41A3 expression was highest in the hemocytes of HYB-UGT41A3, followed by the midgut, whereas expression in HYB was very low. Gradient feeding of BmNPV on HYB-UGT41A3 and control 'HYB' larvae on the first day of the second-instar stage. The results revealed that the LC50 of HYB-UGT41A3 reached 4.040 × 107 particles/mL, which was 20-fold greater than that of HYB. The decrease in the BmNPV load was more significant in HYB-UGT41A3 than in HYB at 48 h after viral inoculation. These results indicate BmUGT41A3 overexpression inhibits BmNPV proliferation and improve resistance to BmNPV in B. mori.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"2"},"PeriodicalIF":2.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of NAC transcription factors from Eremopyrum triticeum promoted abiotic stress tolerance. 小麦中NAC转录因子的过表达促进了非生物胁迫的耐受性。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-30 DOI: 10.1007/s11248-024-00428-3

Xue-Ni Zhong, Jun-Jie Peng, Meng-Yao Wang, Xiu-Li Yang, Li Sun

{"title":"Overexpression of NAC transcription factors from Eremopyrum triticeum promoted abiotic stress tolerance.","authors":"Xue-Ni Zhong, Jun-Jie Peng, Meng-Yao Wang, Xiu-Li Yang, Li Sun","doi":"10.1007/s11248-024-00428-3","DOIUrl":"https://doi.org/10.1007/s11248-024-00428-3","url":null,"abstract":"Eremopyrum triticeum is a typical spring ephemeral species, which in China mainly distributed in the desert regions of northern Xinjiang, and play an important role in the desert ecosystems. E. triticeum has several adaptive characteristics such as short growth rhythms, high photosynthetic efficiency, high seed production, drought and salt resistance. However, the molecular regulatory mechanism of E. triticeum in responses to abiotic stress resistance is still unknown. In this study, two NAC-like transcription factor-encoding genes, EtNAC1 and EtNAC2, were isolated from E. triticeum. The predicted EtNAC1 and EtNAC2 proteins possess a typical NAC DNA-binding domain at the N-terminal region. The qRT-PCR analysis showed that EtNAC1 and EtNAC2 were highly expressed in mature roots of E. triticeum, and were significantly up-regulated under drought, high salt and abscisic acid (ABA) stresses. Subcellular localization analysis in onion epidermal cells revealed that EtNAC1 and EtNAC2 were located in the nucleus. Expression of EtNAC1 and EtNAC2 in yeast cells improved the survival rate of yeast under low temperature, H2O2, high drought and salt stresses. Overexpression of EtNAC1 and EtNAC2 in Arabidopsis thaliana conferred enhanced tolerance to drought and salt stresses, increased ABA sensitivity, and transgenic plants showed higher proline (Pro) content, but lower malondialdehyde content, lower chlorophyll leaching, lower water loss rate and stomatal aperture (width/length) than WT plants. In conclusion, EtNAC1 and EtNAC2 play important roles in abiotic stress responses of E. triticeum, which might have significant potential in crop molecular breeding for abiotic stress tolerance.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"3"},"PeriodicalIF":2.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interplay between Agrobacterium T-DNA and backbone DNA in transgenic plant cells. 农杆菌T-DNA与主链DNA在转基因植物细胞中的相互作用。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-30 DOI: 10.1007/s11248-024-00424-7

Peng Xu, Shaojuan Lai, Bo Yin, Chenyu Yao, Xiaolin Gu, Jilei Huang, Yufei Hu

{"title":"Interplay between Agrobacterium T-DNA and backbone DNA in transgenic plant cells.","authors":"Peng Xu, Shaojuan Lai, Bo Yin, Chenyu Yao, Xiaolin Gu, Jilei Huang, Yufei Hu","doi":"10.1007/s11248-024-00424-7","DOIUrl":"https://doi.org/10.1007/s11248-024-00424-7","url":null,"abstract":"Agrobacterium-mediated transformation of plants often results in the integration of multiple copies of T-DNA and backbone DNA from binary vectors into the host genome. However, the interplay between T-DNA and backbone DNA remains elusive. In this study, 70.8% of T1 Arabidopsis transformants exhibited integration of both T-DNA and backbone DNA, and no cases of only backbone integration were observed. To elucidate the integration patterns, we employed bulk-genome resequencing in Arabidopsis and identified 20 integration sites across 10 T1 transgenic plants, most of which were flanked by left borders of T-DNA at both ends. On average, each integration site contained 6.3 copies of T-DNA and 2.65 copies of backbone DNA. The junction structures between T-DNA and the backbone were highly variable, revealing a previously underappreciated frequency of readthrough at both the left and right borders. Transient expression studies in Nicotiana benthamiana leaves demonstrated that T-DNA and backbone DNA were simultaneously transferred into transformed cells, although the backbone DNA had lower copy numbers than T-DNA. These findings suggest a close relationship between T-DNA and backbone DNA during their transfer and integration, thus offering new insights into the mechanism underlying Agrobacterium-mediated transformation.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"1"},"PeriodicalIF":2.7,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainability requires the integration of farmer knowledge, scientific advancements, and comprehensive innovation. 可持续性要求将农民知识、科学进步和全面创新融为一体。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 Epub Date: 2024-10-21 DOI: 10.1007/s11248-024-00414-9

Urs Niggli

{"title":"Sustainability requires the integration of farmer knowledge, scientific advancements, and comprehensive innovation.","authors":"Urs Niggli","doi":"10.1007/s11248-024-00414-9","DOIUrl":"10.1007/s11248-024-00414-9","url":null,"abstract":"This viewpoint paper emphasises the need to diversify food production methods to simultaneously combat hunger and reduce environmental problems. The recommendations of the UN Food System Summit 2021 relate primarily to (i) the conservation of natural ecosystems, (ii) the sustainable management of existing agricultural land while increasing productivity and (iii) the restoration of already degraded land. Europe in particular faces unique challenges, such as reducing pollution and promoting organic farming up to 25 percent of the agricultural land area while maintaining food production. Ongoing efforts aim to create a transparent, fair and multi-level regulatory framework to support the Green Deal. The implementation of the Corporate Sustainability Reporting Directive (CSRD), which will sooner or later affect a larger proportion of European farmers, should support the transition. Science and innovation play a central role in this, as they are the cornerstones on which sustainable food systems are built. It is imperative that farmers actively participate in the co-design processes and utilise their wealth of experience and creativity to drive these innovations forward. A crucial aspect of the transition to sustainability is changing consumption patterns to limit food waste and reduce meat consumption. While this transition is essential, it is not without its formidable challenges. Diversification of agriculture, encompassing a spectrum of established techniques, is touted as a promising approach to achieving sustainability without sacrificing productivity. Furthermore, integrating truly sustainable agricultural practices with cutting-edge innovations, including new genomic techniques, has the potential to be a transformative solution.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"563-569"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142475604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global conference on sustainability in agriculture & food systems: innovation, indicators and implementation. 农业和粮食系统可持续性：创新、指标和实施全球会议。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 DOI: 10.1007/s11248-024-00418-5

Peter Kearns, Steffi Friedrichs

引用次数: 0

Precision breeding in agriculture and food systems in the United Kingdom. 英国农业和食品系统中的精准育种。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 Epub Date: 2024-08-06 DOI: 10.1007/s11248-024-00397-7

Oli Watson, Sadiye Hayta

引用次数: 0

Limitations in measuring sustainable food systems: domestic policy priorities and global monitoring. 衡量可持续粮食系统的局限性：国内政策重点和全球监测。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 Epub Date: 2024-12-02 DOI: 10.1007/s11248-024-00417-6

Ritsuko Yoneda

引用次数: 0

Narratives in European debate concerning new genomic techniques. 欧洲关于新基因组技术的辩论叙述。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 Epub Date: 2024-12-04 DOI: 10.1007/s11248-024-00416-7

Marcin Napiórkowski, Andrzej Nowak, Mikołaj Biesaga, Szymon Talaga, Erika Staël von Holstein

{"title":"Narratives in European debate concerning new genomic techniques.","authors":"Marcin Napiórkowski, Andrzej Nowak, Mikołaj Biesaga, Szymon Talaga, Erika Staël von Holstein","doi":"10.1007/s11248-024-00416-7","DOIUrl":"10.1007/s11248-024-00416-7","url":null,"abstract":"Given the complexity of agricultural problems, it is essential to develop acceptable solutions for various stakeholders with diverse knowledge, viewpoints, and preferences. However, European public opinion has become highly polarized, making constructive discussions on these issues difficult. We present the results of the narrative analysis of media debate on new genomic techniques. The study identified two primary narrative groups: 'precaution-focused' and 'innovation-focused.' The former emphasizes caution, potential risks, and the need for stringent regulation, while the latter highlights benefits, progress, and the promise of genome editing for sustainable agricultural practices. Within each group of narratives, several distinct narratives were identified. The research has revealed that despite the high polarization, the narratives shared important values and beliefs. Going beyond the dividing narratives and concentrating on common values can depolarize the debate and set the stage for new narratives, enabling constructive debate, concentrating on solving problems, and maximizing collective outcomes.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"551-561"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142772684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of genome edited organisms in Australia. 澳大利亚对基因组编辑生物的监管。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-12-01 Epub Date: 2024-10-24 DOI: 10.1007/s11248-024-00411-y

Peter Thygesen

{"title":"Regulation of genome edited organisms in Australia.","authors":"Peter Thygesen","doi":"10.1007/s11248-024-00411-y","DOIUrl":"10.1007/s11248-024-00411-y","url":null,"abstract":"Whether organisms developed with the use of genome editing techniques, or food derived from such organisms, are, or should be, regulated as genetically modified organisms (GMOs) or genetically modified (GM) food, respectively, remains a subject of debate globally. Much of the discussion has been scientific and focussed on the similar genetic outcomes of some genome editing techniques and 'conventional' or natural mutagenesis. Many jurisdictions, including Australia, have considered, or are considering, how their regulatory frameworks will deal with such organisms and products. In Australia, organisms developed with site directed nuclease 1 (SDN-1, with no added template to guide homology-directed repair) are not regulated as GMOs, pursuant to exclusions in the Gene Technology Regulations 2001. The exclusion of SDN-1 organisms from regulation in Australia is sometimes misrepresented, including in scientific peer reviewed publications, as extending to all genome edited organisms. This highlights the importance for researchers, developers and other stakeholders to understand that whether genome edited organisms are, or are not, subject to regulation as GMOs in a particular jurisdiction may quintessentially be a legal question, not a scientific one.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":" ","pages":"545-550"},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142508720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: WIF1 causes dysfunction of heart in transgenic mice. 更正：WIF1 导致转基因小鼠心脏功能障碍。

IF 2.7 3区生物学

Transgenic Research Pub Date : 2024-10-01 DOI: 10.1007/s11248-024-00403-y

Dan Lu, Wei Dong, Xu Zhang, Xiongzhi Quan, Dan Bao, Yingdong Lu, Lianfeng Zhang

引用次数: 0