Transgenic Research最新文献

Functional analysis of the role of a wound-induced leucine aminopeptidase gene homologue isolated from Rorippa indica in aphid herbivory. 伤致亮氨酸氨基肽酶基因同源物在蚜虫草食中的功能分析。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-05-07 DOI: 10.1007/s11248-026-00492-x

Lekha Bandopadhyay, Debabrata Basu, Samir Ranjan Sikdar

{"title":"Functional analysis of the role of a wound-induced leucine aminopeptidase gene homologue isolated from Rorippa indica in aphid herbivory.","authors":"Lekha Bandopadhyay, Debabrata Basu, Samir Ranjan Sikdar","doi":"10.1007/s11248-026-00492-x","DOIUrl":"https://doi.org/10.1007/s11248-026-00492-x","url":null,"abstract":"Leucine aminopeptidases (LAPs) are multifunctional enzymes with roles in both defence and development. In plants, they are reported to be induced by wound-inflicting Lepidopteran insects and regulate wound response pathways leading to an effective defence response. Infestation by Hemipteran mustard aphid, Lipaphis erysimi (L.) Kaltenbach has been reported to induce wound response as well as a wound-responsive Arabidopsis thaliana Lap1 homologue (RI01; GenBank Accession: JK034053) in Rorippa indica (L.) Hiern. This is interesting as Hemipteran insects like aphids are assumed to inflict minimal wounding. In the present study, starting with the RI01 sequence information, we isolated the full length (1566 bp) sequence of a novel R. indica Lap (RiLap) gene, performed in silico analyses and developed transgenic R. indica plants with suppressed RiLAP activity by expressing a 565 bp antisense fragment of RiLap cDNA. We found that the isolated RiLAP is an acidic LAP of M17 family and suppressing it causes a significant increase in aphid herbivory but reduction in total chlorophyll content and possibly photosynthetic capacity in aphid infested transgenic plants of the T1 generation. These findings though preliminary suggest that RiLap could have a role in deterring aphids by acting as a regulatory protein simultaneously balancing defence response and photosynthetic capacity or plant growth. Noting the dearth of research in this area, this pilot study will be useful for designing future in depth analyses in understanding the role of Laps in defence response against Hemipteran insects. The study has implications in the development of sustainable pest management avenues.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes. cd12 - scon条件敲除小鼠的分子特征揭示了意想不到的剪接变化。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-05-02 DOI: 10.1007/s11248-026-00496-7

Mayke A C Ten Hoor, Margot M Linssen, Conny M Brouwers, Jill W C Claassens, Jaap Mulder, Peter Hohenstein

{"title":"Molecular characterization of Cdh12-SCON conditional knockout mice reveals unexpected splicing changes.","authors":"Mayke A C Ten Hoor, Margot M Linssen, Conny M Brouwers, Jill W C Claassens, Jaap Mulder, Peter Hohenstein","doi":"10.1007/s11248-026-00496-7","DOIUrl":"10.1007/s11248-026-00496-7","url":null,"abstract":"Functional validation of candidate genes in congenital anomalies of the kidneys and urinary tract (CAKUT) and other disorders is essential for translating genetic discoveries into clinical applications. Conditional knockout mouse models are indispensable for studying gene function in complex organ systems. The Short Conditional intrON (SCON) system accelerates the generation of such models by inserting the artificial SCON into a coding exon. SCON is designed to be spliced out after transcription, without affecting gene expression. Upon Cre activity, SCON is converted into the ΔSCON allele which cannot be spliced out, introducing premature termination codons (PTCs) to inactivate the gene. Previous validation of the SCON system in mice has focused primarily on phenotypic outcomes. Here, we provide a molecular characterization of the SCON system in Cdh12-a candidate gene implicated in kidney damage in CAKUT. We found that both Cdh12SCON and Cdh12ΔSCON alleles caused unintended skipping of the exon downstream of the insertion site, culminating in a frameshift and PTC. Consequently, the Cdh12SCON allele led to a ~ 25% reduction in mRNA expression, indicating that it was not transcriptionally inert as designed. Despite unintended exon skipping, the Cdh12ΔSCON allele still effectively suppressed mRNA expression. These findings highlight the importance of transcript-level characterization of engineered alleles prior to functional studies, as artefactual splicing events may occur across multiple gene-targeting strategies, including artificial intron-based conditional alleles as shown here.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on the regulatory mechanisms of the PSY promoter. PSY启动子调控机制的研究进展。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-05-02 DOI: 10.1007/s11248-026-00493-w

Bing-Bing He, Xing-Wen Zhou, Jing-Jing Li, Jie Sun, Yi-Qing Xie, Guo-Yong Han, Jia-Shi Lu

{"title":"Research progress on the regulatory mechanisms of the PSY promoter.","authors":"Bing-Bing He, Xing-Wen Zhou, Jing-Jing Li, Jie Sun, Yi-Qing Xie, Guo-Yong Han, Jia-Shi Lu","doi":"10.1007/s11248-026-00493-w","DOIUrl":"https://doi.org/10.1007/s11248-026-00493-w","url":null,"abstract":"Carotenoids are essential pigments in the plant photosynthetic apparatus, functioning in light harvesting, photoprotection, and signal transduction, and serving as precursors of vital nutrients such as vitamin A. Phytoene synthase (PSY) is the first rate-limiting enzyme in the plant carotenoid biosynthetic pathway, and its transcriptional regulation primarily depends on cis-acting promoter elements, associated transcription factors, and epigenetic status. The PSY promoter region contains core cis-elements as well as multiple light-, hormone-, and stress-responsive elements, which collectively function as key regulatory sites governing spatiotemporal expression. This review systematically summarizes recent advances in PSY promoter regulation by plant hormones (e.g., abscisic acid, ethylene, jasmonic acid), environmental factors (light signaling, temperature, salinity, and drought), and epigenetic mechanisms (DNA methylation, histone modifications, and chromatin remodeling). In addition, the application of transgenic and biotechnological approaches to PSY promoter regulation is further summarized. Including promoter sequence engineering with precise editing of cis-elements and promoter-targeted CRISPR activation/interference (CRISPRa/i) for tunable transcriptional control. Emphasis is placed on how these signals are integrated at the promoter level. Deeper insights into these mechanisms will provide both theoretical foundations and practical strategies for enhancing carotenoid accumulation and stress tolerance in crops through molecular design.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inducing fixation of transgenic alleles in open-pollinated populations. 开放授粉群体中转基因等位基因的诱导固定。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-04-29 DOI: 10.1007/s11248-026-00495-8

Anthony J Conner, Jeanne M E Jacobs

引用次数: 0

Targeted multiplex gene knockouts in Lemna minor using CRISPR/Cas9. 利用CRISPR/Cas9靶向敲除小Lemna的多重基因。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-04-13 DOI: 10.1007/s11248-026-00488-7

Sadegh Shojaei Baghini, Kasra Esfahani, Nima Rad, Mahdi Arezoumandi, Elham Taghipour, Ali Hatef Salmanian

{"title":"Targeted multiplex gene knockouts in Lemna minor using CRISPR/Cas9.","authors":"Sadegh Shojaei Baghini, Kasra Esfahani, Nima Rad, Mahdi Arezoumandi, Elham Taghipour, Ali Hatef Salmanian","doi":"10.1007/s11248-026-00488-7","DOIUrl":"https://doi.org/10.1007/s11248-026-00488-7","url":null,"abstract":"Lemna minor (commonly known as duckweed) is a fast-growing aquatic plant recognized as a promising green bioreactor for recombinant protein production. Its rapid proliferation, high protein yield, environmental adaptability, and edibility make it highly attractive for biotechnological applications. It is essential to develop and expand genetic tools tailored to this species to maximize these advantages and further unlock its biotechnological potential. A key strategy for achieving this goal is the implementation of advanced genome editing technologies, such as the CRISPR/Cas9 system. Although multiplex CRISPR/Cas9 gene editing has previously been successfully applied in Lemna aequinoctialis, the capability of the endogenous plant tRNA processing system for multiplex editing in L. minor using the polycistronic tRNA-sgRNA (PTG)/Cas9 system has not yet been explored. In this study, a PTG construct was engineered to include four sgRNAs designed to simultaneously target two plant-specific glycosyltransferase genes: α-1,3-fucosyltransferase (FucT) and β-1,2-xylosyltransferase (XylT). As anticipated, the PTG-Cas9 system successfully induced frameshift mutations, characterized by insertions and deletions (indels), in regenerated L. minor plants derived from transformed calli. Validation via PCR and RT-PCR analysis, followed by sequencing of the target loci, confirmed the presence of indels at the target sites. Furthermore, western blot analyses utilizing antibodies specific to XylT and FucT in two homozygous lines (lines 44 and 217) revealed truncated XylT proteins in both lines. Moreover, an in-frame FucT protein was detected in line 217, whereas FucT expression was absent in line 44. This study marked the first successful demonstration of PTG-Cas9 system for multiplex genome editing in L. minor, paving the way for advanced genetic engineering in this species.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147677010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and characterization of the Il11-Cre knock-in mouse. Il11-Cre敲入小鼠的产生和表征。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-04-07 DOI: 10.1007/s11248-026-00491-y

Xuetao Zhou, Zhongliang Xie, Wenjia Liu, Qingye Wang, Huisang Lin, Rongyu Li, Yiming Lang, Jun Chen, Xiao Yang, Yan Teng, Guan Yang

引用次数: 0

CRISPR/Cas9-mediated knockout of PsLykX gene of pea (Pisum sativum L.) leads to loss of symbiotic nodules. CRISPR/ cas9介导的敲除豌豆（Pisum sativum L.） PsLykX基因导致共生根瘤的丢失。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-04-02 DOI: 10.1007/s11248-026-00490-z

Igor Yu Zhuravlev, Anton A Lyakhovets, Andrew G Matveenko, Maria A Lebedeva, Aleksandr I Zhernakov, Veronika Y Simonova, Anton S Sulima, Igor A Tikhonovich, Vladimir A Zhukov

{"title":"CRISPR/Cas9-mediated knockout of PsLykX gene of pea (Pisum sativum L.) leads to loss of symbiotic nodules.","authors":"Igor Yu Zhuravlev, Anton A Lyakhovets, Andrew G Matveenko, Maria A Lebedeva, Aleksandr I Zhernakov, Veronika Y Simonova, Anton S Sulima, Igor A Tikhonovich, Vladimir A Zhukov","doi":"10.1007/s11248-026-00490-z","DOIUrl":"https://doi.org/10.1007/s11248-026-00490-z","url":null,"abstract":"Pea (Pisum sativum L.) symbiosis with nodule bacteria supplying plants with additional nitrogen is a very specific plant-microbial interaction. Mutual recognition of the partners occurs through perception of bacterial signal molecules (Nod factors) by plant receptors, enabling bacterial entry via root hairs and formation of nitrogen-fixing nodules. The pea gene Sym2, described but not yet cloned, exists in different allelic forms defining the symbiotic specificity, and is therefore thought to encode a Nod factor receptor. The PsLykX gene is a strong candidate for the Sym2, since its alleles coincide with high or low symbiotic specificity; however, to date, no genetic evidence has been obtained for a role of PsLykX in symbiosis. Here, we knocked-out the PsLykX in European pea cultivar Caméor using Agrobacterium-mediated hairy root transformation and CRISPR-Cas9 editing. The roots with editing events confirmed by sequencing lost the ability to form nodules, providing direct functional evidence that PsLykX is essential, at least, for the symbiosis between pea cultivar Caméor and Rhizobium ruizarguesonis RCAM1026.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amylase/trypsin inhibitors (ATIs) levels in wheat event IND-ØØ412-7 are similar to non-transgenic wheat. 小麦事件IND-ØØ412-7中淀粉酶/胰蛋白酶抑制剂（ATIs）水平与非转基因小麦相似。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-03-19 DOI: 10.1007/s11248-026-00487-8

Antonella Ferela, Francisco Ayala, Patricia V Miranda

{"title":"Amylase/trypsin inhibitors (ATIs) levels in wheat event IND-ØØ412-7 are similar to non-transgenic wheat.","authors":"Antonella Ferela, Francisco Ayala, Patricia V Miranda","doi":"10.1007/s11248-026-00487-8","DOIUrl":"10.1007/s11248-026-00487-8","url":null,"abstract":"Despite being a main protein supplier in the human diet, wheat proteins represent a health challenge for some people. Besides the well-known celiac disease caused by gluten proteins, there is an occupational illness known as baker´s asthma. Amylase/trypsin inhibitors (ATIs) have been reported to be the major group of wheat proteins responsible for bakers´ asthma. As part of the characterization of stress-tolerant (HB4® technology) transgenic wheat (event IND-ØØ412-7, HB4 wheat), the level of the seven ATIs (0.28, 0.19 + 0.53, CM2, CM3, CM16, and CM17) was determined and compared to non-transgenic varieties. The materials tested in this study included the transgenic event in two different genetic backgrounds, their conventional counterparts (cv. Algarrobo and cv. Basilio), and five additional commercial varieties. Grain samples were obtained from field trials in Argentina in 2020 at six different locations. No significant differences were found in the ATIs levels between HB4 wheat and its isolines. ATIs levels in HB4 wheat were then analyzed within the natural variation given by the varieties and locations included in this study. Altogether, this study confirmed that ATIs levels, as previously reported for other allergens, are mainly affected by the genetic background and the environmental conditions, and that the ATIs levels measured in HB4 transgenic wheat are within the range of natural variability observed in the non-transgenic counterparts.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13002686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of wheat Wcor80 confers tolerance to low temperature in transgenic plant. 小麦Wcor80基因的过表达使转基因植株具有耐低温性。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-03-19 DOI: 10.1007/s11248-026-00489-6

Luyao Wang, Jiaang Cao, Xuesong Wang, Junbao Zhang, Yushu Chen, Sen Yang, Qingyi Shao, Mengdi Yu, Zhongmin Jin, Lijie Liu

引用次数: 0

The ladder-shape melting temperature isothermal amplification methods for detection of CaMV 35S and CP4 EPSPS genes in soybean foods. 建立了梯子型熔化温度等温扩增检测大豆食品中CaMV 35S和CP4 EPSPS基因的方法。

IF 2 3区生物学

Transgenic Research Pub Date : 2026-03-04 DOI: 10.1007/s11248-025-00464-7

Wei Yao, Juan Tian, Dan Zhou, Shanlu Guo, Yajie Wang, Jinxin Liu, Chunmei Song, Xiaohua Zhang, Deguo Wang, Yongzhen Wang, Yao Wang, Bailing Yin

{"title":"The ladder-shape melting temperature isothermal amplification methods for detection of CaMV 35S and CP4 EPSPS genes in soybean foods.","authors":"Wei Yao, Juan Tian, Dan Zhou, Shanlu Guo, Yajie Wang, Jinxin Liu, Chunmei Song, Xiaohua Zhang, Deguo Wang, Yongzhen Wang, Yao Wang, Bailing Yin","doi":"10.1007/s11248-025-00464-7","DOIUrl":"10.1007/s11248-025-00464-7","url":null,"abstract":"Soybean has become the most widely grown genetically modified (GM) crop in the world and it is often used to produce food and feed. With the rapid development of GM technology, there is growing controversy over the safety of GM foods. It is important to develop the methods for detecting GM products rapidly and conveniently. In this paper, the LMTIA method was combined with the Proofman probes for detection of soybean, CaMV 35S soybean and CP4 EPSPS soybean. It was shown that the optimal reaction temperatures for soybean, CaMV 35S soybean and CP4 EPSPS soybean were 59, 61 and 59 °C, respectively, and the sensitivities were correspondingly 10, 100 and 100 pg/µL. The nucleic acid amplification can be completed in 20 min. For the nine non-GM soybean foods tested, neither the Proofman-LMTIA method nor the digital PCR had detected the CaMV 35S and CP4 EPSPS genes, which were consistent with the label claims. The LMTIA method was established for the rapid detection of transgenic soybeans.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0