Transgenic Research最新文献

Construction and evaluation of an independently generated transgenic mouse model carrying mutated human HRAS genes for short-term carcinogenicity assessment. 携带人类HRAS突变基因的转基因小鼠模型的构建及短期致癌性评估

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-09-03 DOI: 10.1007/s11248-026-00510-y

Wen Zeng, Yanlin Zhang, Xiaoliu Yang, Xiaoyu Li, Yunlong Jiang, Dongjing Jia, Juan Liang, Tao Wang

{"title":"Construction and evaluation of an independently generated transgenic mouse model carrying mutated human HRAS genes for short-term carcinogenicity assessment.","authors":"Wen Zeng, Yanlin Zhang, Xiaoliu Yang, Xiaoyu Li, Yunlong Jiang, Dongjing Jia, Juan Liang, Tao Wang","doi":"10.1007/s11248-026-00510-y","DOIUrl":"https://doi.org/10.1007/s11248-026-00510-y","url":null,"abstract":"The study aimed to construct and evaluate an independently generated transgenic mouse model applied to the short-term carcinogenicity assessment. Mutated human HRAS fragment containing an intron point-mutation was inserted into C57BL/6JGpt mice via bacterial artificial chromosome transgenic technology, eventually generating BALB/c;B6J-Tg(hHRAS)16/Gpt mice, abbreviated as HRAS mice. The inserted human HRAS fragment in HRAS mice was characterized, revealing five tandem copies at chromosome 19. Baseline profiles, including biochemical, hematological, immunophenotypic, survival, and carcinogenic data of HRAS mice, were collected. To evaluate the tumor susceptibility in HRAS mice, we applied N-Nitroso-N-methylurea (MNU) to HRAS mice in a short-term carcinogenicity assessment conducted according to Good Laboratory Practice. The genetic characteristics of HRAS mice include five tandem arrays of mutated human HRAS fragments located in genomic coordinate 7,755,606 on chromosome 19 and the duplication of a 9-kilobase genome sequence (genomic coordinate 7,755,606-7,746,509) located on chromosome 19. HRAS mice showed a relatively lower incidence and range of spontaneous tumor formation during long-term observation compared to CByB6F1-Tg(HRAS)2Jic (Tg.rasH2) transgenic mice. The short-term carcinogenicity assessment showed a strong tumor response to MNU, with high incidences of lymphoma (≥ 90%) and stomach squamous cell papilloma (≥ 90%) in both male and female HRAS mice. The HRAS mice showed susceptibility to MNU and exhibited baseline characteristics distinct from those of Tg.rasH2 mice. The co-expression of HRAS and MKI67 at the cellular localization level was found in neoplasms of HRAS mice. These findings preliminarily evaluated the feasibility of HRAS mice applied to the short-term carcinogenicity assessment.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A stable and potent buffalo EF1α1 promoter for robust gene expression in mammalian systems. 一个稳定有效的水牛EF1α1启动子在哺乳动物系统中健壮的基因表达。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-08-03 DOI: 10.1007/s11248-026-00507-7

Satarupa Dutta, Abhishek Das, Subhra Dutta, Amisha Bhattacharya, Goutam Ulgekar, Shankhamala Chatterjee, Amit Pal, Srimoyee Koner, Mariya Saify, Swarnali Khan, Soma Behera, Nirmalya Ganguli

{"title":"A stable and potent buffalo EF1α1 promoter for robust gene expression in mammalian systems.","authors":"Satarupa Dutta, Abhishek Das, Subhra Dutta, Amisha Bhattacharya, Goutam Ulgekar, Shankhamala Chatterjee, Amit Pal, Srimoyee Koner, Mariya Saify, Swarnali Khan, Soma Behera, Nirmalya Ganguli","doi":"10.1007/s11248-026-00507-7","DOIUrl":"https://doi.org/10.1007/s11248-026-00507-7","url":null,"abstract":"This study reports the first isolation and characterization of the buffalo EF1α1 promoter, demonstrating its strong gene expression activity both in vitro across diverse cultured cell types and in vivo across multiple mouse organs. Although viral promoters, such as cytomegalovirus (CMV) and simian virus (SV40), are widely used for their strong expression in various cell lines in mammalian expression systems and in animal tissues, they are prone to methylation-induced transcriptional silencing and subsequent loss of exogenous gene expression. The most effective alternative to viral promoters is the synthetic hybrid CAG promoter (cytomegalovirus major immediate-early enhancer combined with the chicken beta-actin promoter) or mammalian cellular promoter such as human elongation factor 1 alpha (hEF1α), which drives strong gene expression but lacks consistency and is limited in their in vivo expression potential due to their vulnerability to epigenetic silencing. To overcome these challenges, the bbEF1α1 promoter was cloned and evaluated both in vitro and in vivo. It consistently drives higher levels of exogenous gene expression than CMV in diverse cell lines. Importantly, transgene expression was achieved in various organs of transgenic mice and in muscle tissue following in vivo electroporation. These findings establish the bbEF1α1 promoter as a powerful ubiquitous driver of gene expression, offering high stability with broad applications in gene therapy, biopharmaceutical production, and functional genomics.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148664440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An efficient floral dipping transformation method for the metal hyperaccumulator Noccaea caerulescens. 一种高效的金属超积累植物毛毡花浸渍转化方法。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-07-14 DOI: 10.1007/s11248-026-00506-8

Jitpanu Yamjabok, Elisabeth C M van der Heijden, Maarten Koornneef, Henk Schat, Mark G M Aarts

{"title":"An efficient floral dipping transformation method for the metal hyperaccumulator Noccaea caerulescens.","authors":"Jitpanu Yamjabok, Elisabeth C M van der Heijden, Maarten Koornneef, Henk Schat, Mark G M Aarts","doi":"10.1007/s11248-026-00506-8","DOIUrl":"10.1007/s11248-026-00506-8","url":null,"abstract":"Noccaea caerulescens is a metal hyperaccumulator plant species with the capacity to accumulate high concentrations of zinc (Zn), cadmium (Cd) and nickel (Ni). Several candidate genes have so far been associated with accumulation and tolerance of these metals, but gene function analysis has been cumbersome in the absence of an efficient stable plant transformation method for N. caerulescens. A previously identified mutation in the FLOWERING LOCUS C gene, conferring early flowering to the calamine accession St. Felix de Pallières, has been introgressed in the genetic background of five different genotypes, originating from the calamine populations Clough Wood (CLW) and Le Blémard (BLE), the ultramafic population Cira (CIR), and the non-metallicolous populations St. Baudille (SBD) and Werschmatt (WER). Agrobacterium tumefaciens mediated floral dipping transformation was employed in five introgression lines in these backgrounds and in Arabidopsis thaliana as control. Three introgression lines, of genetic backgrounds CLW, BLE and SBD, were successfully transformed, achieving an average transformation efficiency ≥ 0.29% for each background. A reproducible, stable transformation method based on floral dipping is presented here as an improvement on previously reported methods. The early flowering N. caerulescens flc mutation can be readily introgressed into different genetic backgrounds to facilitate genetic transformation studies, which is expected to provide a highly desired extra genetic tool to study gene function analysis in the metal hyperaccumulator N. caerulescens.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13368838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148438017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic expression of a Penicillium oxalicum cyclophilin gene in Arabidopsis thaliana imparts tolerance to cold stress. 拟南芥中草青霉亲环蛋白基因的异位表达赋予其对冷胁迫的耐受性。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-07-07 DOI: 10.1007/s11248-026-00504-w

Anantika Suri, Mangaljeet Singh, Anish Kaachra, Prabhjeet Singh

引用次数: 0

A perspective on the reliability and relevance of research results. 对研究结果的可靠性和相关性的看法。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-07-07 DOI: 10.1007/s11248-026-00503-x

J F Buyel

引用次数: 0

Correction: Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases. 更正：建立用于创建人类疾病条件和生理相关模型的Cre-rat资源。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-06-30 DOI: 10.1007/s11248-026-00501-z

Huimin Zhang, Qi Zheng, Ruby Yanru Chen-Tsai

引用次数: 0

Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective. CRISPR技术对农业可持续性的影响：全球应用和土耳其视角。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-06-27 DOI: 10.1007/s11248-026-00505-9

Zeki Bayramoğlu

{"title":"Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.","authors":"Zeki Bayramoğlu","doi":"10.1007/s11248-026-00505-9","DOIUrl":"10.1007/s11248-026-00505-9","url":null,"abstract":"This review evaluates CRISPR/Cas applications in agriculture from a global perspective with explicit reference to Türkiye. Using a literature gap-matrix approach organised around four analytical dimensions-environmental, economic, social and policy, and scientific and technological-we synthesize the primary evidence on water and input use, productivity, disease resistance, and product quality. The literature concentrates on water and fertilizer use, productivity, and off-target accuracy, whereas soil health, biodiversity, consumer acceptance, ethical considerations and regulatory frameworks remain systematically under-represented. Global deployment of CRISPR is already delivering measurable advantages in food security, shelf life and nutritional value, while in Türkiye the research base is at an early stage but has clear potential in wheat, barley, tomato and olive. Translating CRISPR into Turkish agricultural sustainability requires (i) a domestic biosafety framework aligned with the emerging European New Genomic Techniques approach, (ii) sustained investment in multi-location primary field trials, and (iii) inclusive deployment mechanisms-particularly through producer cooperatives-that allow smallholder farmers to benefit from edited varieties.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148346319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mammalian growth factors enhance regeneration in transgenic tomato lines. 哺乳动物生长因子促进转基因番茄植株再生。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-06-19 DOI: 10.1007/s11248-026-00502-y

Cecile Garchery, Juliette Benejam, Alexia Grau, Justine Gricourt, Esther Pelpoir, Mathilde Causse

{"title":"Mammalian growth factors enhance regeneration in transgenic tomato lines.","authors":"Cecile Garchery, Juliette Benejam, Alexia Grau, Justine Gricourt, Esther Pelpoir, Mathilde Causse","doi":"10.1007/s11248-026-00502-y","DOIUrl":"https://doi.org/10.1007/s11248-026-00502-y","url":null,"abstract":"Genome editing technologies are now available for many crop species, greatly enhancing our ability to investigate gene function and transforming the field of plant transgenesis. However, the capacity to regenerate whole plants from cell culture remains a major limiting factor in many crops. Even in species with regeneration potential, certain genotypes remain recalcitrant. The physiological state of plant cells plays a central role in growth and development and is closely associated with kinase-mediated signaling networks. Notably, several defense-related genes activated during cellular repair processes following transgenesis share significant homology with mammalian defense genes. In this study, we evaluated whether supplementation with three mammalian growth factors could enhance regeneration efficiency in tomato. We selected two cytokines and a pro-inflamatory factor showing homology with plant kinase genes. We compared the percentage of transgenic plants generated through CRISPR-Cas9-mediated mutagenesis of four genes involved in sugar and organic acid metabolism across six tomato lines exhibiting varying regeneration capacities. Over three years of transformation experiments, we demonstrated that the addition of mammalian growth factors during transgenesis significantly improved regeneration frequency, particularly in recalcitrant tomato genotypes. Furthermore, growth factor supplementation not only enhanced transformation efficiency in difficult-to-transform lines but also increased the production of stable secondary lines.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148278876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transgenic expression of human signal regulatory protein alpha (SIRPα) in BALB/c Rag2^null/Jak3^null immunodeficient mice enhances human lymphoma xenografts by reducing phagocytosis. 人信号调节蛋白α (SIRPα)在BALB/c Rag2null/Jak3null免疫缺陷小鼠中的转基因表达通过减少吞噬作用增强人淋巴瘤异种移植物。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-06-15 DOI: 10.1007/s11248-026-00500-0

Jutatip Panaampon, Tatsuya Ogawa, Akira Shiota, Seiji Okada

{"title":"Transgenic expression of human signal regulatory protein alpha (SIRPα) in BALB/c Rag2null/Jak3null immunodeficient mice enhances human lymphoma xenografts by reducing phagocytosis.","authors":"Jutatip Panaampon, Tatsuya Ogawa, Akira Shiota, Seiji Okada","doi":"10.1007/s11248-026-00500-0","DOIUrl":"10.1007/s11248-026-00500-0","url":null,"abstract":"The immunodeficient mouse model offers a platform for evaluating therapeutic options in preclinical cancer research by enabling the growth of human xenografts. We previously developed BALB/c Rag2nullJak3null (BRJ), an immunodeficient mouse model useful for cancer studies. However, susceptibility to xenograft acceptance can be increased through signal regulatory protein alpha (SIRPα)-CD47 signaling, also known as the \"don't eat me\" signal. In this study, we generated BALB/c human-SIRPα BAC transgenic mice and crossed them with BRJ mice. The resulting human SIRPα-transgenic BRJ (BRJ-S) mice showed a significantly higher engraftment rate of human B-cell lymphoma than BRJ mice. Furthermore, we demonstrated better human tumor engraftment in BRJ-S mice by reducing phagocytosis. In summary, hSIRPα-transgenic BRJ mice (BRJ-S) serve as a promising immunodeficient model with enhanced capacity for human lymphoma engraftment. BRJ-S provides an advantageous and permissive model for xenografting and for studying cancer in research and drug development.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13265592/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148253450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of different mutagens in tuberose (Polianthes tuberosa) cut flower. 不同诱变剂对薯蓣切花的影响。

IF 1.9 3区生物学

Transgenic Research Pub Date : 2026-05-18 DOI: 10.1007/s11248-026-00499-4

Mehrdad Babarabie, Ali Salehi-Sardoei, Babak Jamali, Bahman Fazeli-Nasab, Mehrnaz Hatami, Mansour Ghorbanpour

{"title":"Evaluation of different mutagens in tuberose (Polianthes tuberosa) cut flower.","authors":"Mehrdad Babarabie, Ali Salehi-Sardoei, Babak Jamali, Bahman Fazeli-Nasab, Mehrnaz Hatami, Mansour Ghorbanpour","doi":"10.1007/s11248-026-00499-4","DOIUrl":"10.1007/s11248-026-00499-4","url":null,"abstract":"Various techniques are used to introduce new cultivars, especially in ornamental species like tuberose, which have limited genetic diversity. This study employed a completely randomized design with three replications to investigate the effects of different mutagens on mutation induction in tuberose bulbs. The mutagens tested included sodium azide at concentrations of 200, 300, and 400 mg L-1, ethyl methanesulfonate at 0.25, 0.5, and 0.75 mg L-1, and gamma rays at doses of 10, 20, and 30 Gy. After exposure to the mutagens, both mother and daughter bulbs were grown for two consecutive years under greenhouse conditions. Our results indicated an increase in the number of florets in bulbs treated with sodium azide, regardless of the concentration used. The largest floret diameter, measuring 47.82 mm, was recorded in samples treated with ethyl methanesulfonate at a concentration of 0.5 mg L-1. This same concentration also resulted in an increase in the reducing sugar content within the petals. The longest vase life, recorded at 8.66 days, was observed in samples exposed to gamma radiation at a dose of 10 Gy. Significant changes in enzymatic antioxidants were noted in bulbs treated with the different mutagens, both before and after harvest. In conclusion, all three mutagens had an influence on the growth of tuberose plants, affecting various morphological, physiological, and postharvest parameters.","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"35 1","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147964679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0