Harit K Bal, Collin J Preftakes, Lawrence Long, Carlos J Esquivel, Frankie Stubbins, Chitvan Khajuria, Jianguo Tan, David Dyck, Brent Werner, Justin Ungerer, Bingyao Li, Yu Liu-Gontarek, Chen Meng, Yong Yin, Steven L Levine, Tianbo Xu, Christopher R Brown
{"title":"Cry1Da_7、Cry1B生态风险评价3和Vip3Cb1蛋白在具有鳞翅目靶向活性的防虫棉MON 89151中的表达","authors":"Harit K Bal, Collin J Preftakes, Lawrence Long, Carlos J Esquivel, Frankie Stubbins, Chitvan Khajuria, Jianguo Tan, David Dyck, Brent Werner, Justin Ungerer, Bingyao Li, Yu Liu-Gontarek, Chen Meng, Yong Yin, Steven L Levine, Tianbo Xu, Christopher R Brown","doi":"10.1007/s11248-025-00460-x","DOIUrl":null,"url":null,"abstract":"<p><p>An ecological risk assessment (ERA) was conducted for MON 89151, which expresses three proteins (Cry1Da_7, Cry1B.3, and Vip3Cb1) developed to help protect against lepidopteran pests such as Heliothis virescens, Helicoverpa zea, and Spodoptera frugiperda. The ERA focused on evaluating the potential risks to beneficial non-target organisms (NTOs) from MON 89151 cultivation, by examining the protein's mode of action, insecticidal activity spectrum, ecological exposure levels, potential for environmental persistence, and hazard to representative NTO taxa under laboratory conditions. The protection goal driving the ERA was preserving key ecosystem services provided by NTOs in agriculture. The Cry1Da_7 and Cry1B.3 proteins, the same and/or similar to previously registered (Cry1Da_7 in MON 95379 maize) and/or reviewed (Cry1B.2 in MON 94637 soybean) insecticidal proteins, have been demonstrated to pose negligible risks to NTOs, enabling a bridging approach to existing hazard testing for these proteins. The third protein in MON 89151, Vip3Cb1 also demonstrated no adverse effects on NTOs at or above expected environmental concentrations under laboratory conditions. Therefore, the ERA concluded that cultivation of MON 89151 would pose minimal ecological risk to NTOs, supporting its safety in agricultural ecosystems.</p>","PeriodicalId":23258,"journal":{"name":"Transgenic Research","volume":"34 1","pages":"42"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12441081/pdf/","citationCount":"0","resultStr":"{\"title\":\"Ecological risk assessment for Cry1Da_7, Cry1B.3 and Vip3Cb1 proteins expressed in MON 89151 cotton: an insect-protected cotton with targeted activity against Lepidoptera.\",\"authors\":\"Harit K Bal, Collin J Preftakes, Lawrence Long, Carlos J Esquivel, Frankie Stubbins, Chitvan Khajuria, Jianguo Tan, David Dyck, Brent Werner, Justin Ungerer, Bingyao Li, Yu Liu-Gontarek, Chen Meng, Yong Yin, Steven L Levine, Tianbo Xu, Christopher R Brown\",\"doi\":\"10.1007/s11248-025-00460-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An ecological risk assessment (ERA) was conducted for MON 89151, which expresses three proteins (Cry1Da_7, Cry1B.3, and Vip3Cb1) developed to help protect against lepidopteran pests such as Heliothis virescens, Helicoverpa zea, and Spodoptera frugiperda. The ERA focused on evaluating the potential risks to beneficial non-target organisms (NTOs) from MON 89151 cultivation, by examining the protein's mode of action, insecticidal activity spectrum, ecological exposure levels, potential for environmental persistence, and hazard to representative NTO taxa under laboratory conditions. The protection goal driving the ERA was preserving key ecosystem services provided by NTOs in agriculture. The Cry1Da_7 and Cry1B.3 proteins, the same and/or similar to previously registered (Cry1Da_7 in MON 95379 maize) and/or reviewed (Cry1B.2 in MON 94637 soybean) insecticidal proteins, have been demonstrated to pose negligible risks to NTOs, enabling a bridging approach to existing hazard testing for these proteins. The third protein in MON 89151, Vip3Cb1 also demonstrated no adverse effects on NTOs at or above expected environmental concentrations under laboratory conditions. 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Ecological risk assessment for Cry1Da_7, Cry1B.3 and Vip3Cb1 proteins expressed in MON 89151 cotton: an insect-protected cotton with targeted activity against Lepidoptera.
An ecological risk assessment (ERA) was conducted for MON 89151, which expresses three proteins (Cry1Da_7, Cry1B.3, and Vip3Cb1) developed to help protect against lepidopteran pests such as Heliothis virescens, Helicoverpa zea, and Spodoptera frugiperda. The ERA focused on evaluating the potential risks to beneficial non-target organisms (NTOs) from MON 89151 cultivation, by examining the protein's mode of action, insecticidal activity spectrum, ecological exposure levels, potential for environmental persistence, and hazard to representative NTO taxa under laboratory conditions. The protection goal driving the ERA was preserving key ecosystem services provided by NTOs in agriculture. The Cry1Da_7 and Cry1B.3 proteins, the same and/or similar to previously registered (Cry1Da_7 in MON 95379 maize) and/or reviewed (Cry1B.2 in MON 94637 soybean) insecticidal proteins, have been demonstrated to pose negligible risks to NTOs, enabling a bridging approach to existing hazard testing for these proteins. The third protein in MON 89151, Vip3Cb1 also demonstrated no adverse effects on NTOs at or above expected environmental concentrations under laboratory conditions. Therefore, the ERA concluded that cultivation of MON 89151 would pose minimal ecological risk to NTOs, supporting its safety in agricultural ecosystems.
期刊介绍:
Transgenic Research focusses on transgenic and genome edited higher organisms. Manuscripts emphasizing biotechnological applications are strongly encouraged. Intellectual property, ethical issues, societal impact and regulatory aspects also fall within the scope of the journal. Transgenic Research aims to bridge the gap between fundamental and applied science in molecular biology and biotechnology for the plant and animal academic and associated industry communities.
Transgenic Research publishes
-Original Papers
-Reviews:
Should critically summarize the current state-of-the-art of the subject in a dispassionate way. Authors are requested to contact a Board Member before submission. Reviews should not be descriptive; rather they should present the most up-to-date information on the subject in a dispassionate and critical way. Perspective Reviews which can address new or controversial aspects are encouraged.
-Brief Communications:
Should report significant developments in methodology and experimental transgenic higher organisms