Ecological risk assessment for Cry1Da_7, Cry1B.3 and Vip3Cb1 proteins expressed in MON 89151 cotton: an insect-protected cotton with targeted activity against Lepidoptera.

IF 2 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Harit K Bal, Collin J Preftakes, Lawrence Long, Carlos J Esquivel, Frankie Stubbins, Chitvan Khajuria, Jianguo Tan, David Dyck, Brent Werner, Justin Ungerer, Bingyao Li, Yu Liu-Gontarek, Chen Meng, Yong Yin, Steven L Levine, Tianbo Xu, Christopher R Brown
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Abstract

An ecological risk assessment (ERA) was conducted for MON 89151, which expresses three proteins (Cry1Da_7, Cry1B.3, and Vip3Cb1) developed to help protect against lepidopteran pests such as Heliothis virescens, Helicoverpa zea, and Spodoptera frugiperda. The ERA focused on evaluating the potential risks to beneficial non-target organisms (NTOs) from MON 89151 cultivation, by examining the protein's mode of action, insecticidal activity spectrum, ecological exposure levels, potential for environmental persistence, and hazard to representative NTO taxa under laboratory conditions. The protection goal driving the ERA was preserving key ecosystem services provided by NTOs in agriculture. The Cry1Da_7 and Cry1B.3 proteins, the same and/or similar to previously registered (Cry1Da_7 in MON 95379 maize) and/or reviewed (Cry1B.2 in MON 94637 soybean) insecticidal proteins, have been demonstrated to pose negligible risks to NTOs, enabling a bridging approach to existing hazard testing for these proteins. The third protein in MON 89151, Vip3Cb1 also demonstrated no adverse effects on NTOs at or above expected environmental concentrations under laboratory conditions. Therefore, the ERA concluded that cultivation of MON 89151 would pose minimal ecological risk to NTOs, supporting its safety in agricultural ecosystems.

Abstract Image

Cry1Da_7、Cry1B生态风险评价3和Vip3Cb1蛋白在具有鳞翅目靶向活性的防虫棉MON 89151中的表达
对表达Cry1Da_7、Cry1B和Cry1Da_7三种蛋白的mon 89151进行了生态风险评估(ERA)。3和Vip3Cb1)的开发有助于防止鳞翅目害虫,如Heliothis virescens, Helicoverpa zea和Spodoptera frugiperda。本研究通过检测mon89151蛋白的作用模式、杀虫活性谱、生态暴露水平、环境持久性潜力以及在实验室条件下对代表性NTO分类群的危害,重点评估了mon89151培养对有益非靶生物(NTOs)的潜在风险。推动ERA的保护目标是保护nto在农业中提供的关键生态系统服务。Cry1Da_7和Cry1B。3个蛋白,相同和/或类似于先前注册的(Cry1Da_7在MON 95379玉米)和/或审查的(Cry1B。(2在mon94637大豆中)杀虫蛋白,已被证明对NTOs构成可忽略不计的风险,从而实现了对这些蛋白质现有危害测试的桥接方法。在实验室条件下,mon89151中的第三个蛋白Vip3Cb1在预期或更高的环境浓度下也没有对NTOs产生不利影响。因此,ERA认为种植mon89151对nto的生态风险最小,支持其在农业生态系统中的安全性。
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来源期刊
Transgenic Research
Transgenic Research 生物-生化研究方法
CiteScore
5.40
自引率
0.00%
发文量
38
审稿时长
4-8 weeks
期刊介绍: Transgenic Research focusses on transgenic and genome edited higher organisms. Manuscripts emphasizing biotechnological applications are strongly encouraged. Intellectual property, ethical issues, societal impact and regulatory aspects also fall within the scope of the journal. Transgenic Research aims to bridge the gap between fundamental and applied science in molecular biology and biotechnology for the plant and animal academic and associated industry communities. Transgenic Research publishes -Original Papers -Reviews: Should critically summarize the current state-of-the-art of the subject in a dispassionate way. Authors are requested to contact a Board Member before submission. Reviews should not be descriptive; rather they should present the most up-to-date information on the subject in a dispassionate and critical way. Perspective Reviews which can address new or controversial aspects are encouraged. -Brief Communications: Should report significant developments in methodology and experimental transgenic higher organisms
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