First report of transgenic Agave sisalana 'RLV 19' plants resistant to the herbicide glyphosate-an improved alternative for biofuel production.

IF 2 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Carolina Rossi de Oliveira, Aline Vitória Corim Marim, Camila Santos Woloche, Paulo Cezar De Lucca, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira
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引用次数: 0

Abstract

The Agave genus is recognized for its diversity, economic importance, and adaptability to arid and semi-desert climates. Among its species, Agave sisalana stands out for the production of sisal, a resilient natural fiber with various industrial applications and potential to be used as a raw material in biofuel production, due to the accumulation of fermentable sugars in its biomass. Although this species presents significant agronomic potential, challenges in conventional breeding hinder its cultivation. A viable alternative for genetic improvement is the development of transgenic plants that incorporate desirable agronomic traits through the controlled insertion of genes of interest into their genome. The objective of study was to develop a protocol for obtaining transgenic plants of Agave sisalana 'RLV19,' a species widely cultivated in the semi-arid region of Bahia-BA, Brazil, using the cp4-epsps gene aimed at constitutive gene expression. Two transgenic plants of Agave sisalana 'RLV19' were regenerated via organogenesis. PCR analyses and CP4-EPSP protein expression by RT-PCR confirmed the presence and expression of the transgene in these plants. This is the first report of A. sisalana transgenic plants expressing the cp4-epsps gene, and to the best of our knowledge, there have been no prior reports on protocols for the production of transgenic plants of this species.

转基因龙舌兰“RLV 19”抗草甘膦除草剂的首次报道——一种改进的生物燃料生产替代品。
龙舌兰属以其多样性、经济重要性和对干旱和半沙漠气候的适应性而闻名。剑麻是一种有弹性的天然纤维,具有多种工业应用,由于其生物质中积累了可发酵的糖,因此有可能被用作生物燃料生产的原料。尽管该物种具有显著的农艺潜力,但传统育种方面的挑战阻碍了其栽培。一种可行的遗传改良替代方法是开发转基因植物,通过控制将感兴趣的基因插入其基因组,将理想的农艺性状纳入其中。本研究的目的是利用cp4-epsps基因对组成型基因的表达,建立一种利用在巴西巴伊亚-巴伊亚半干旱地区广泛种植的龙舌兰RLV19转基因植株的方案。通过器官发生再生了2株转基因龙舌兰‘RLV19’。PCR分析和RT-PCR的CP4-EPSP蛋白表达证实了该基因在这些植物中的存在和表达。这是首次报道表达cp4-epsps基因的剑麻转基因植物,据我们所知,之前没有关于该物种转基因植物生产方案的报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Transgenic Research
Transgenic Research 生物-生化研究方法
CiteScore
5.40
自引率
0.00%
发文量
38
审稿时长
4-8 weeks
期刊介绍: Transgenic Research focusses on transgenic and genome edited higher organisms. Manuscripts emphasizing biotechnological applications are strongly encouraged. Intellectual property, ethical issues, societal impact and regulatory aspects also fall within the scope of the journal. Transgenic Research aims to bridge the gap between fundamental and applied science in molecular biology and biotechnology for the plant and animal academic and associated industry communities. Transgenic Research publishes -Original Papers -Reviews: Should critically summarize the current state-of-the-art of the subject in a dispassionate way. Authors are requested to contact a Board Member before submission. Reviews should not be descriptive; rather they should present the most up-to-date information on the subject in a dispassionate and critical way. Perspective Reviews which can address new or controversial aspects are encouraged. -Brief Communications: Should report significant developments in methodology and experimental transgenic higher organisms
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