Transplantation proceedings最新文献

{"title":"Cryopreserved Skin Transplantation in a Nonhuman Primate Model","authors":"Meghan Hu ,&nbsp;Rafaela Belloni ,&nbsp;Joseph Ladowski ,&nbsp;Davide Schiliro ,&nbsp;Alessandro Martinino ,&nbsp;Allison Schwalb ,&nbsp;Zishen Li ,&nbsp;Mingqing Song ,&nbsp;Kyha Williams ,&nbsp;Alton B. Farris ,&nbsp;Stuart Knechtle ,&nbsp;Jean Kwun","doi":"10.1016/j.transproceed.2025.06.002","DOIUrl":"10.1016/j.transproceed.2025.06.002","url":null,"abstract":"<div><h3>Background</h3><div>Skin transplantation is often used in nonhuman primate (NHP) transplant research for sensitization and to assess tolerance. However, the availability of donor animals for skin can be constrained after organ donation. In this study, we evaluated the feasibility of cryopreserving skin patches to address this issue.</div></div><div><h3>Methods</h3><div>A full-thickness dorsal skin section with a diameter of ∼3 cm was excised. Following defatting process, the skin graft was immersed in a skin-cryopreservation media and preserved in liquid nitrogen. Prior to transplantation, the frozen skin was rapidly thawed using a 37°C water bath and thoroughly washed with normal saline. Cryopreserved syngeneic skin transplantations were performed, monitored, and assessed histologically.</div></div><div><h3>Results</h3><div>Autologous skin grafts preserved in liquid nitrogen with prolonged storage time (&gt;1 month) demonstrated successful engraftment in NHP recipients. The cryopreserved skin exhibited well-preserved epidermis and dermis, with minimal distinction compared to non-cryopreserved samples. In fully engrafted cryopreserved skin grafts, dermal fibrosis appeared slightly less distinct at 28 days after transplantation. These differences could be attributable to anatomical location of the samples.</div></div><div><h3>Conclusion</h3><div>The cryopreserved skin showed well-preserved normal skin histology before and after skin transplantation. Here, we show that cryopreserved NHP skin can be stored for a prolonged time and grafted in a delayed manner. Cryopreserved skin could serve as a source when a living donor NHP is unavailable, including for testing donor-specific tolerance in the absence of the organ donor.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1201-1204"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis and Visualization of Research Trends and Hotspots in the Body Donation Field","authors":"Yunfeng Wang ,&nbsp;Luqing Zhang","doi":"10.1016/j.transproceed.2025.05.015","DOIUrl":"10.1016/j.transproceed.2025.05.015","url":null,"abstract":"<div><h3>Introduction</h3><div>The development of body donation (BD) programs has varied significantly worldwide, with recent increases in donations. This study performed a visual analysis of BD research over the past 2 decades to explore trends and key areas of interest within the field.</div></div><div><h3>Methods</h3><div>This study used scientometric data from the Web of Science database and 2 bibliometric analysis tools: VOSviewer and CiteSpace.</div></div><div><h3>Results</h3><div>Between 2004 and 2024, 1384 BD articles were published in 568 journals. Publication and citation numbers increased consistently. Leading contributions come from institutions in Europe and the United States. The countries, institutions, journals, and authors with the highest publication counts included the United States (342), Medical University of Innsbruck (55), Anatomical Sciences Education (117), and Raffaele De Caro (26). The most frequently used keywords were BD, anatomy, dissection, attitude, medical education, transplantation, and organ donation. Keywords that showed significant bursts included outcomes, whole BD, educators, gross anatomy education, disease, in vitro studies, governing BD, adipose tissue, respect, and legal considerations.</div></div><div><h3>Conclusions</h3><div>The findings suggest that future research topics and hotspots in the BD field should focus on evaluating teaching effectiveness, enhancing medical ethics, improving legal frameworks, fostering public education and awareness, and advancing tissue engineering transplantation.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 953-965"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144487590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Protocolized Double-J Catheter Use on the Incidence of Urinary Tract Infection in the Immediate Postkidney Transplant Period","authors":"Sammara Azevedo Guedes ,&nbsp;Clara Manuela Silva Sampaio ,&nbsp;Gabriel Brayan Gutiérrez-Peredo ,&nbsp;Iris Montaño-Castellón ,&nbsp;Andrea Jimena Gutiérrez-Peredo ,&nbsp;Fernanda Pinheiro Martin Tapioca ,&nbsp;Milena Sampaio Barreto Machado ,&nbsp;Marcelo Barreto Lopes ,&nbsp;Ricardo José Costa Mattoso","doi":"10.1016/j.transproceed.2025.06.007","DOIUrl":"10.1016/j.transproceed.2025.06.007","url":null,"abstract":"<div><h3>Introduction</h3><div>Urological complications after renal transplantation, particularly urinary tract infections (UTIs), significantly affect graft survival and quality of life. Double-J catheters are used to prevent complications, but their impact on UTI development remains unclear. This study evaluates protocolized double-J catheter (PDJC) use in the first 3 months post-transplant, examining associated complications and the influence of catheter duration.</div></div><div><h3>Methods</h3><div>This retrospective, quasi-experimental study analyzed kidney transplant recipients aged ≥18 years from 2021 to 2023, with patient assignment determined by timing of transplantation relative to institutional protocol implementation. Non-PDJC patients were transplanted in 2021 to 2022, while PDJC patients received transplants in 2023 following updated institutional protocols. Logistic regression analysis identified UTI risk factors, with associations expressed as odds ratios (OR) with 95% confidence intervals (CI). A logistic regression was performed with both unadjusted and adjusted models to assess the association between the absence of PDCJ use and UTI as confounding variables.</div></div><div><h3>Results</h3><div>Among 202 renal transplant patients analyzed (mean age 45.5 ± 13 years, 58% male, 93% non-white), overall UTI incidence was 38%, with significantly lower rates in the PDJC group compared to controls (31% vs 45%, <em>P</em> = .046). Short-term catheter placement (≤10 days) achieved the lowest UTI rate at 25%, whereas prolonged catheter duration (≥30 days) was associated with increased infection frequency. Multivariable analysis identified female sex as an independent risk factor for UTI development (OR = 2.29; 95% CI: 1.28; 4.09, <em>P</em> = .005). The absence of PDJC nearly doubled UTI risk (OR = 1.80; 95% CI: 1.0; 3.18) after controlling for potential confounders.</div></div><div><h3>Conclusion</h3><div>PDJC use for ≤10 days was associated with reduced UTI risk in the immediate post-transplant period, while female sex emerged as an independent risk factor. These findings suggest that shorter catheter duration protocols may help optimize infection prevention strategies following kidney transplantation.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1034-1040"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Why Living Kidney Donors Are Declined: A Swiss Single Center Observation Over 15 Years","authors":"Christian Kuhn ,&nbsp;Aurelia Schnyder ,&nbsp;Wolfgang Ender ,&nbsp;Anna Wiegand ,&nbsp;Patrick Folie ,&nbsp;Isabelle Binet ,&nbsp;Alexander Ritter","doi":"10.1016/j.transproceed.2025.06.009","DOIUrl":"10.1016/j.transproceed.2025.06.009","url":null,"abstract":"<div><h3>Introduction</h3><div>Living kidney donation plays a crucial role in addressing organ shortage. Careful and holistic donor assessment is required to minimize donor risks. While acceptance patterns are well documented, donor candidates beyond the threshold remain largely unexamined. This study aims to analyze donor evaluations to identify reasons and trends for donor decline.</div></div><div><h3>Methods</h3><div>In this retrospective observational study, we analyzed prospectively collected data of 275 potential living kidney donor evaluated at our center between 2007 and 2021, assessing demographic and health characteristics, as well as the reasons for donor decline.</div></div><div><h3>Results</h3><div>Of the 275 donor candidates evaluated, 197 (71.6%) were declined. The primary reasons were medical and surgical issues (32.7%), particularly kidney-related conditions (9.5%), followed by immunological factors (21.1%) and psychosocial concerns (12.7%). Interestingly, there was a trend towards accepting medically more complex donors over the study period, with an increase in mean donor age and lower estimated glomerular filtration rate of accepted donors.</div></div><div><h3>Conclusion</h3><div>Donor decline is influenced by a range of medical, immunological, and psychosocial factors. These findings highlight the importance of individualized assessments and call for further research into overcoming donation barriers and evaluating long-term outcomes, particularly for medically complex donors, to optimize safe and effective living kidney donation.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1041-1049"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory Effects and Pharmacokinetics of Bupivacaine in Transversus Abdominis Plane Block for Living Liver Donors: A Prospective Randomized Clinical Trial","authors":"Mehmet Ridvan Yalin ,&nbsp;Mehmet Ali Erdogan ,&nbsp;Muharrem Ucar ,&nbsp;Muhammed Mehdi Uremis ,&nbsp;Yusuf Ziya Colak ,&nbsp;Emine Ozdemir ,&nbsp;Yilmaz Ugur ,&nbsp;Ulku Ozgul ,&nbsp;Selim Erdogan ,&nbsp;Osman Kacmaz ,&nbsp;Duygu Demiroz ,&nbsp;Huseyin Ilksen Toprak","doi":"10.1016/j.transproceed.2025.06.003","DOIUrl":"10.1016/j.transproceed.2025.06.003","url":null,"abstract":"<div><h3>Objective</h3><div>Transversus abdominis plane (TAP) blocks have been reported to decrease postoperative pain and opioid use. Local anesthetics modulate the local and systemic inflammatory reaction. Our aim was to examine the effect of TAP block with bupivacaine on the anti-inflammatory response in living liver donors.</div></div><div><h3>Methods</h3><div>The randomized prospective clinical study was carried out on 72 living liver donors, aged 18 to 65 scheduled for right hepatectomy. TAP blocks were performed bilaterally with ultrasound guidance using 1.5 mg/kg of 0.5% bupivacaine for group 2. Group 1 patients were the control group. In both groups, blood samples were obtained preoperatively, 30 minutes and 2, 6, and 24 hours after the injection for cytokine measurement. In group 2, to examine the plasma bupivacaine level, blood samples were obtained preoperatively as well as 30 minutes and 1, 2, 6, 12, and 24 hours after injection. The primary outcome was to assess the effect of TAP block on the inflammatory response using cytokine levels.</div></div><div><h3>Results</h3><div>No difference was observed between cytokine levels (tumor necrosis factor-alpha, interleukin [IL]-1 and IL-6) between groups Significant positive correlations were observed between the plasma bupivacaine concentration and IL-1 and IL-6 levels at 2, 6, and 24 hours.</div></div><div><h3>Conclusion</h3><div>Although the TAP block did not affect the levels of cytokines significantly, a significant association was seen between the concentrations of plasma bupivacaine and the levels of IL-1 and IL-6 at 2, 6, and 24 hours. In contrast, the clinical significance of this association is still under investigation.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1095-1103"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144577502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced SARS-CoV-2 BA.2.86 Neutralization After BA.5 Infection in Vaccinated Kidney Transplant Recipients","authors":"Keita Kawashiro ,&nbsp;Kiyohiko Hotta ,&nbsp;Rigel Suzuki ,&nbsp;Naoya Iwahara ,&nbsp;Takayuki Hirose ,&nbsp;Shuhei Tsujino ,&nbsp;Takasuke Fukuhara ,&nbsp;Nobuo Shinohara","doi":"10.1016/j.transproceed.2025.05.021","DOIUrl":"10.1016/j.transproceed.2025.05.021","url":null,"abstract":"<div><h3>Background</h3><div>Vaccination with mRNA vaccines has significantly reduced the SARS-CoV-2 mortality rate in the general population. However, the effectiveness of mRNA vaccines in kidney transplant (KTx) recipients is unclear.</div></div><div><h3>Methods</h3><div>In this cohort, we compared the disease severity and seroconversion rate after SARS-CoV-2 infection in 30 vaccinated and 8 unvaccinated KTx recipients. KTx recipients have infected between February 2022 and September 2023 during the Omicron variant phases. We measured anti-SARS-CoV-2 spike protein IgG antibodies (cutoff value was 1.0 AU/mL). Furthermore, we investigated anti-SARS-CoV-2 spike protein IgG antibodies and the neutralizing antibody titer against BA.5 and BA.2.86 in 10 vaccinated KTx recipients before and after BA.5 infection.</div></div><div><h3>Result</h3><div>The incidence of moderate disease was significantly higher in the unvaccinated group (<em>P</em> = .004). The median antibody titers after SARS-CoV-2 infection in vaccinated and unvaccinated KTx recipients were 244.5 (IQR: 43.5–757.8) and &lt;1 (IQR: &lt;1–&lt;1) AU/mL, respectively (<em>P</em> &lt; .0001). Surprisingly, none of the 5 patients with moderate disease developed detectable antibodies after infection. The antibody titers and neutralizing antibody titer against BA.5 and BA.2.86 variants in vaccinated KTx recipients increased significantly after BA.5 infection (S-IgG: <em>P</em> = .004, BA.5: <em>P</em> = .002, BA.2.86: <em>P</em> = .016).</div></div><div><h3>Conclusions</h3><div>Although vaccinated KTx recipients achieved IgG antibody and neutralizing antibody boost after SARS-CoV-2 infection, unvaccinated KTx recipients did not experience an increase in antibody titers and experienced more severe infections. Furthermore, KTx recipients acquired the neutralizing activity against Omicron BA.2.86 after Omicron BA.5 infection. Thus, vaccination should be recommended for KTx recipients.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1013-1017"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Association Between AMR and Airway Complications Among Lung Transplant Recipients","authors":"Zehra Dhanani ,&nbsp;Alonso Marquez ,&nbsp;Omar Al Omari ,&nbsp;Fatima Anjum","doi":"10.1016/j.transproceed.2025.06.006","DOIUrl":"10.1016/j.transproceed.2025.06.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Airway complications (AC) such as stenosis and dehiscence are major challenges following lung transplantation, occurring in 1.4% to 44% of recipients and often linked to donor bronchial ischemia. Despite advances in surgical and bronchoscopic techniques, predictors for AC still remain unclear. This study aimed to evaluate the prevalence of AC and identify potential risk factors in a contemporary cohort.</div></div><div><h3>Methods</h3><div>We retrospectively analyzed 220 lung transplant recipients (2020–2023) at a high-volume center. Airway complications were categorized (stenosis, dehiscence, ischemia/necrosis, malacia) per ISHLT guidelines. Baseline characteristics and outcomes were compared between patients with and without AC using chi-squared and Wilcoxon rank-sum tests. Multivariate logistic regression evaluated associations with factors such as antibody-mediated rejection (AMR), microbial colonization, surgical techniques, and clinical outcomes.</div></div><div><h3>Results</h3><div>Airway complications were observed in 17.2% of patients, with stenosis (12.7%) being the most common. Among those with stenosis, 18 patients (8.2% of the total cohort) had preceding ischemia/necrosis noted on earlier surveillance bronchoscopy. Malacia occurred in 3.1% of the patients. A combination of 2 or more complications was identified in 1.3% of patients. Multivariate analysis revealed that AMR significantly increased the risk of AC (adjusted odds ratio [aOR] 5.554, <em>P</em> = .013). Additional risk factors included Pseudomonas colonization (aOR 2.728, <em>P</em> = .014), Aspergillus colonization (aOR 3.623, <em>P</em> = .026), and the telescoping surgical technique (<em>P</em> = .038) in the comparative analysis.</div></div><div><h3>Conclusion</h3><div>Airway complications remain prevalent despite advancements in lung transplantation. AMR, microbial colonization, and the telescoping technique are significant contributors to AC. These findings underscore the need for early intervention and further research into immunological mechanisms to improve patient outcomes.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1127-1132"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144546748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Protective Effect and Regulatory Mechanism of Plumbagin on Renal Ischemia-Reperfusion Injury Based on the Nrf-2/HO-1 Signaling Pathway","authors":"Qixun Luo ,&nbsp;Zunwei Zhu ,&nbsp;Lisong Wan","doi":"10.1016/j.transproceed.2025.05.028","DOIUrl":"10.1016/j.transproceed.2025.05.028","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate and validate the protective role of plumbagin (PL) in renal ischemia-reperfusion injury (IRI) by mediating the Nrf2/HO-1 pathway and reducing renal function damage, thereby providing a theoretical basis for the application of PL in the prevention of renal IRI.</div></div><div><h3>Methods</h3><div>Twenty-four healthy adult male BALB/c mice were randomly divided into four groups: the sham group, the IR model group (IRI group), the IRI + PL 10 mg/kg group, and the IRI + PL 10 mg/kg + ML385 (30 mg/kg) group. Four groups of mice were given drugs 2 weeks before modeling, for 2 consecutive weeks (once a day), and the modeling was started 1 hour after the administration on the 14th day. In the sham operation group, only the renal vessels were isolated without clamping ischemia, and in the other groups, minimally invasive artery clamps were used to establish the mouse RIRI injury model. Serum creatinine (Scr), blood urea nitrogen (BUN), interleukin-6 (IL-6), myeloperoxidase (MPO), serum superoxide dismutase (SOD), GSH-Px, malondialdehyde (MDA), and inflammatory levels of mice in each group were detected and analyzed; pathological examination of renal tissue injury; and the expressions of Nrf-2 and HO-1 were detected by Western blotting.</div></div><div><h3>Results</h3><div>Compared with the sham group, the IRI group showed elevated Scr, BUN, IL-6, MDA, and MPO levels, reduced SOD and GSH-Px activities, severe renal injury, and decreased Nrf-2/HO-1 expression. PL administration reduced renal injury, decreased Scr, BUN, IL-6, MDA, and MPO levels, increased SOD and GSH-Px levels, and increased Nrf-2/HO-1 expression, as confirmed by pathology and Western blotting.</div></div><div><h3>Conclusions</h3><div>PL can reduce the level of oxidative stress and the release of inflammatory factors by regulating the Nrf-2/HO-1 signaling pathway, improve renal injury induced by ischemia/reperfusion in mice, and play a renoprotective role.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1180-1186"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144510205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penehyclidine Hydrochloride Improves Ischemia-Reperfusion Injury in Lung Transplantation by Inhibiting Inflammatory Response and Oxidative Stress Injury","authors":"Rongfang Liu ,&nbsp;Qiaoying Cai ,&nbsp;Yongle Li ,&nbsp;Xuguang Zhang","doi":"10.1016/j.transproceed.2025.06.004","DOIUrl":"10.1016/j.transproceed.2025.06.004","url":null,"abstract":"<div><h3>Objectives</h3><div>Penehyclidine hydrochloride, a common anticholinergic drug, shows protective effects on ischemia-reperfusion injury (IRI) in various organs. However, the effects of penehyclidine hydrochloride on lung IRI induced by lung transplantation (LTx) have not been known, especially during the cold ischemia phase. Therefore, this study aimed to explore the effect of penehyclidine hydrochloride on lung IRI in a rat model of LTx.</div></div><div><h3>Material and methods</h3><div>Rats were randomly divided into sham group, control group, and penehyclidine hydrochloride group (PHC group). Rats in the sham group only underwent thoracotomies without LTx. Rats in the C group and PHC group established LTx model and received no treatment or penehyclidine hydrochloride respectively during the cold ischemia phase. At 120 minutes after LTx, the blood gas analysis, wet to dry ration (W/D), inflammatory reaction, oxidative stress injury, and lung structure were detected. Additionally, the type II alveolar epithelial cells observed by transmission electron microscopy were also explored.</div></div><div><h3>Results</h3><div>Compared with C group, the oxygenation, W/D, inflammatory markers, oxidative stress markers and lung structure in the C group and PHC group deteriorated, which improved in the PHC group compared with those in the C group (<em>P</em> &lt; .05). Additionally, the type II alveolar epithelial cells were also less damaged in the PHC group compared with the C group (<em>P</em> &lt; .05).</div></div><div><h3>Conclusions</h3><div>Penehyclidine hydrochloride applied during the cold ischemia phase improved lung IRI caused by LTx via inhibiting inflammatory response and oxidative stress injury, and maintained the integrity of organizational structure in the type II alveolar epithelial cells.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1120-1126"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperative Risk Factors for Early Acute Kidney Injury After Living Donor Liver Transplantation: A Single Transplant Center in Vietnam","authors":"Ngo Dinh Trung MD, PhD ,&nbsp;Do Van Nam ,&nbsp;Nguyen Thi Thu ,&nbsp;Nguyen Tai Thu ,&nbsp;Ho Nam ,&nbsp;Hoang Xuan Su","doi":"10.1016/j.transproceed.2025.05.022","DOIUrl":"10.1016/j.transproceed.2025.05.022","url":null,"abstract":"<div><h3>Background</h3><div>Early acute kidney injury (AKI) is a frequent and severe complication following liver transplantation, significantly influencing both short-term and long-term outcomes of liver transplant recipients. However, data regarding perioperative risk factors for AKI in living donor liver transplantation remain limited.</div></div><div><h3>Methods</h3><div>This retrospective study analyzed 97 adult patients who underwent living donor liver transplantation at Central Military Hospital 108, Hanoi, Vietnam. AKI was defined by the Kidney Disease: Improving Global Outcomes criteria. Clinical and laboratory data were recorded from the electronic medical profile. The risk factors for AKI development were recognized using logistic regression analysis.</div></div><div><h3>Results</h3><div>Among 97 liver transplant recipients, the incidence of AKI post LT was 63.9% (62/97). Preoperative MELD score (odds ratio [OR], 1.07; 95% confidence interval [CI], 1.01–1.13; <em>P</em> = .019), cold ischemia time (OR, 1.03; 95% CI, 1.00–1.07; <em>P</em> = .050), intraoperative blood loss volume (OR, 1.81; 95% CI, 1.05–3.09; <em>P</em> = .032), and serum lactate within the first 6 hours after transplant (OR, 1.61; 95% CI, 1.21–2.15; <em>P</em> = .001) were identified as independent risk factors for early AKI. Patients with AKI experienced prolonged intensive care unit and hospital stays, higher rates of renal replacement therapy, and increased 1-year post-transplant mortality compared with non-AKI patients.</div></div><div><h3>Conclusion</h3><div>Preoperative Model for End-Stage Liver Disease score, cold ischemia time, intraoperative blood loss, and early postoperative serum lactate levels are independent risk factors for early AKI. Early AKI is associated with worse clinical outcomes, underscoring the importance of targeted interventions to mitigate the risk of AKI development.</div></div>","PeriodicalId":23246,"journal":{"name":"Transplantation proceedings","volume":"57 6","pages":"Pages 1063-1070"},"PeriodicalIF":0.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144532164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}