Transactions of The Royal Society of Tropical Medicine and Hygiene最新文献_第6页

Proteogenomic approaches for snake venom protein-based drug development: current trends and challenges. 基于蛇毒蛋白的药物开发的蛋白质基因组学方法：当前趋势和挑战。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/trae073

Sandeep Kumar Jhade, Karthik Kalidoss, Poonam Kumari Pathak, Rahul Shrivastava

{"title":"Proteogenomic approaches for snake venom protein-based drug development: current trends and challenges.","authors":"Sandeep Kumar Jhade, Karthik Kalidoss, Poonam Kumari Pathak, Rahul Shrivastava","doi":"10.1093/trstmh/trae073","DOIUrl":"10.1093/trstmh/trae073","url":null,"abstract":"Snake venom proteins have long been recognized for their therapeutic potential. Proteogenomic strategies, integrating transcriptomics and proteomics, have emerged as powerful tools for identifying and characterizing venom proteins for the development of novel therapeutic agents. Analytical techniques like mass spectrometry and next-generation sequencing enable comprehensive analysis, identifying key venom components and their variants. Recent studies unveil the diversity and complexity of snake venom, highlighting species-specific variations in toxin composition. Structural biology techniques, including x-ray crystallography and cryo-electron microscopy, provide insights into venom enzyme structures and mechanisms of action, facilitating drug design. Bioinformatics tools aid in data analysis and prediction of venom protein functions, enhancing drug discovery efforts. Despite advancements, challenges persist, including toxicity, formulation stability and clinical validation. This review describes the current as well as future options for research and development and emphasizes the critical role of proteogenomic techniques in developing snake venom protein-based drugs.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":"638-647"},"PeriodicalIF":1.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142923224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of repeat testing in tuberculosis patients with a trace call on Xpert MTB/RIF Ultra: a retrospective cohort study. Xpert MTB/RIF Ultra对肺结核患者重复检测的影响：一项回顾性队列研究

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/traf002

Suyash Jha, Nitin Gupta, Tirlangi Praveen Kumar, Kiran Chawla, Vishnu P Shenoy, Muralidhar Varma, Mohan K Manu

引用次数: 0

The impact of multiple infections and community knowledge on engagement with a historical deworming programme: hookworm and Ascaris in Jamaica, 1913-1936. 多重感染和社区知识对参与历史驱虫规划的影响：牙买加钩虫和蛔虫，1913-1936。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/traf010

Jonathan David Roberts, Lorna L Waddington, Rupert J Quinnell, Alison M Dunn

{"title":"The impact of multiple infections and community knowledge on engagement with a historical deworming programme: hookworm and Ascaris in Jamaica, 1913-1936.","authors":"Jonathan David Roberts, Lorna L Waddington, Rupert J Quinnell, Alison M Dunn","doi":"10.1093/trstmh/traf010","DOIUrl":"10.1093/trstmh/traf010","url":null,"abstract":"Introduction: Community engagement with public health efforts often depends on existing knowledge of a health issue.Methods: Here, qualitative analysis of archival material from the Jamaica Hookworm Commission (1919-1936) and quantitative analysis of prevalence data are used to assess knowledge of and ecological interactions between different helminths during a historical hookworm eradication campaign.Results: Archival sources demonstrate that Jamaicans were familiar with Ascaris lumbricoides. Surveys revealed a high prevalence of hookworm (62% of individuals infected), Ascaris (30%) and Trichuris trichiura (32%) in communities targeted for hookworm control. Community prevalence of Trichuris was positively associated with the prevalence of Ascaris and hookworm. Many individuals were infected with more than one parasite. At an individual level, data from hospital patients and soldiers showed significant associations between all three parasites. The co-occurrence of hookworm and Ascaris, alongside folk treatment of Ascaris with the same plant used by the Hookworm Commission (Chenopodium ambrisoides) to treat hookworm, made biomedical claims about hookworm credible and biomedical treatment more acceptable. Expulsions of Ascaris following treatment also provided dramatic proof of the effectiveness of treatment, further facilitating engagement.Discussion/conclusion: Knowledge of Ascaris and other helminths directly shaped engagement with hookworm treatment, demonstrating how folk medical knowledge, grounded in the biology of the worms, aided a biomedical public health program.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":"586-595"},"PeriodicalIF":1.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12138884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143400142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time PCR-HRM assay for precise identification of Entamoeba species in diarrheal samples: clinical validation in tropical health settings. 实时PCR-HRM法精确鉴定腹泻样品中的内阿米巴：热带卫生机构的临床验证。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/traf059

Priya Datta, Divya Rattan, Puja Garg, Sadhna Lal Bhasin, Pankaj Malhotra, Surinder Rana, Sumeeta Khurana, Rakesh Sehgal

{"title":"Real-time PCR-HRM assay for precise identification of Entamoeba species in diarrheal samples: clinical validation in tropical health settings.","authors":"Priya Datta, Divya Rattan, Puja Garg, Sadhna Lal Bhasin, Pankaj Malhotra, Surinder Rana, Sumeeta Khurana, Rakesh Sehgal","doi":"10.1093/trstmh/traf059","DOIUrl":"https://doi.org/10.1093/trstmh/traf059","url":null,"abstract":"Background: Amoebiasis, caused by Entamoeba histolytica, is a major health concern in tropical regions like India. Stool microscopy, the primary diagnostic method, has limited sensitivity due to morphological similarities with Entamoeba dispar and Entamoeba moshkovskii, an emerging pathogen. This study evaluates the effectiveness of quantitative polymerase chain reaction with high-resolution melting (qPCR-HRM) in distinguishing these morphologically similar Entamoeba species.Methods: The qPCR-HRM method was standardized using control strains of E. histolytica, E. dispar and E. moshkovskii. The assay was further evaluated on 150 stool samples, with species confirmation achieved through conventional PCR and Sanger sequencing.Results: The melting peaks of E. histolytica and E. moshkovskii were at 80±2°C and 82±2°C, respectively, and for E. dispar at 69±2°C. The qPCR-HRM was able to detect as low as 10 fg of parasitic DNA. Of 150 stool samples, a total of 10 (6.6%) were found to be positive for E. histolytica, 13 (8.6%) for E. dispar and 7 (4.6%) for E. moshkovskii.Conclusions: This study is the first to standardize qPCR-HRM for the detection and differentiation of Entamoeba species from India. The qPCR-HRM assay offers a sensitive, specific and cost-effective diagnostic tool, contributing to improved patient management.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of geo-climatic factors on the prevalence and diversity of tick fauna in Achai cattle of the northern Hindukush Mountains, Khyber pakhtunkhwa, Pakistan. 地理气候因素对巴基斯坦开伯尔-普赫图赫瓦省兴都库什山脉北部阿柴牛蜱虫动物群的流行和多样性的影响。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/traf020

Israr Khan, Zia Ur Rehman Khalil, Muhammad Shuaib, Sami Ullah, Irfan Ullah, Obaid Ullah, Shahrood Ahmed Siddiqui, Anthony Pokoo-Aikins, Majid S Jabir, Ayman A Swelum

{"title":"Impact of geo-climatic factors on the prevalence and diversity of tick fauna in Achai cattle of the northern Hindukush Mountains, Khyber pakhtunkhwa, Pakistan.","authors":"Israr Khan, Zia Ur Rehman Khalil, Muhammad Shuaib, Sami Ullah, Irfan Ullah, Obaid Ullah, Shahrood Ahmed Siddiqui, Anthony Pokoo-Aikins, Majid S Jabir, Ayman A Swelum","doi":"10.1093/trstmh/traf020","DOIUrl":"10.1093/trstmh/traf020","url":null,"abstract":"Background: Ticks are blood-feeding ectoparasites that can transmit different diseases in livestock and humans. Globally, 994 tick species are recognized, belonging to three families, that is, Argasidae (220 species), Ixodidae (773 species) and Nuttalliellidae (one species). The current study investigated the impact of geo-climatic conditions on the prevalence and distribution of ticks in Achai cattle of the northern Hindukush Mountains of Khyber Pakhtunkhwa.Methods: A total of 707 households containing Achai cattle were investigated for the prevalence and distribution of ticks in Achai cattle.Results: The overall prevalence of positive households (388) was 53.46%. Different geo-climatic conditions, such as location, humidity, temperature and altitude, showed a significant impact on the prevalence and distribution of tick fauna. Higher tick prevalence was observed in Achai animals kept at altitudes of 1500-2000 m, 41-50% humidity and 10-20oC. The most prevalent tick species was Rhipicephalus microplus (67.26%), followed by Haemaphysalis montgomeryi (16.75%), under all studied geo-climatic conditions.Conclusions: Achai cattle are mostly susceptible to the tick species R. microplus, which might be due to its richness in the study area. Furthermore, the results regarding the impact of geo-climatic conditions on the diversity and richness of the tick species could be considered in preventive policies for tick control.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":"613-622"},"PeriodicalIF":1.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Burden of mycetoma in Burkina Faso: case series and systematic review. 布基纳法索足菌肿负担：病例系列和系统评价。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-05 DOI: 10.1093/trstmh/traf015

Abdourahmane Ouangré, Isidore W Yerbanga, Ibrahim Savadogo, Hamado Ouédraogo, Nebon Delphine Bado, André Nagalo, Fagnima Traoré, Abdoul Kader Dem, Sibiri Abdoul Karim Bouda, Djima Fahriane Dora Koulybari, Abdoulaye Sawadogo, Seydou Nakanabo Diallo, Jean-Pierre Gangneux, Ahmed Hassan Fahal, Sanata Bamba

{"title":"Burden of mycetoma in Burkina Faso: case series and systematic review.","authors":"Abdourahmane Ouangré, Isidore W Yerbanga, Ibrahim Savadogo, Hamado Ouédraogo, Nebon Delphine Bado, André Nagalo, Fagnima Traoré, Abdoul Kader Dem, Sibiri Abdoul Karim Bouda, Djima Fahriane Dora Koulybari, Abdoulaye Sawadogo, Seydou Nakanabo Diallo, Jean-Pierre Gangneux, Ahmed Hassan Fahal, Sanata Bamba","doi":"10.1093/trstmh/traf015","DOIUrl":"10.1093/trstmh/traf015","url":null,"abstract":"Mycetoma is a chronic inflammatory disease characterized by progressive and devastating granulomatous subcutaneous tissue involvement. It primarily occurs in regions classified within the 'mycetoma belt', encompassing various countries. Notably, West African nations, including Burkina Faso (BF), are situated within this belt, yet there is a significant lack of data regarding mycetoma in these regions. With this background, this study set out to determine the mycetoma prevalence in BF. A comprehensive review was conducted to document the presence of mycetoma in BF. Searches were performed in PubMed, ScienceDirect, African Journals Online (AJOL) and Google Scholar databases. Data were reported following the Preferred Reporting Items for Systematic and Meta-analyses (PRISMA) guidelines. Additionally, the study encompassed a case series involving three patients. From 1960 to 2024, 25 mycetoma cases have been documented in BF. Overall, the clinical cases share similar sociodemographic, clinical, diagnostic, therapeutic and outcome characteristics. Specifically, these cases illustrate that mycetoma predominantly affects vulnerable groups in BF. The majority of mycetoma cases (96%) were reported in the Sahelian zone of BF. Predominantly, these cases (80%) had foot mycetoma. This study highlights the challenges of mycetoma management in BF, indicating significant obstacles in early diagnosis and effective treatment.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":"570-577"},"PeriodicalIF":1.9,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding leprosy: immune response, clinical manifestations and emerging diagnostic approaches. 解码麻风病：免疫反应，临床表现和新兴诊断方法。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-06-04 DOI: 10.1093/trstmh/traf058

Héctor Serrano-Coll, Lina Restrepo-Rivera, Yicenia Cuadros-Urrego, Nora Cardona-Castro

引用次数: 0

Seroprevalence and risk factors of Anisakis simplex in Makoko schoolchildren: insights from a Lagos, Nigeria slum. 马科科学童单纯异尖线虫的血清患病率和危险因素：来自尼日利亚拉各斯贫民窟的见解。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-05-29 DOI: 10.1093/trstmh/traf055

Chia-Kwung Fan, Ting-Wu Chuang, Hon-Ian Lei, Vincent P Gyang, Tyng-Shiuan Hsieh, Po-Ching Cheng, Chia-Mei Chou, Olaoluwa P Akinwale

{"title":"Seroprevalence and risk factors of Anisakis simplex in Makoko schoolchildren: insights from a Lagos, Nigeria slum.","authors":"Chia-Kwung Fan, Ting-Wu Chuang, Hon-Ian Lei, Vincent P Gyang, Tyng-Shiuan Hsieh, Po-Ching Cheng, Chia-Mei Chou, Olaoluwa P Akinwale","doi":"10.1093/trstmh/traf055","DOIUrl":"https://doi.org/10.1093/trstmh/traf055","url":null,"abstract":"Background: Anisakis simplex is a parasitic zoonosis associated with consuming raw or undercooked fish. Despite its global relevance, data from Africa are scarce. This study assessed the seroprevalence and risk factors of A. simplex infection among primary schoolchildren (PSC) in Makoko, an urban slum in Lagos, Nigeria.Methods: A cross-sectional study was conducted from November 2013 to March 2014 involving 196 PSC from three government schools. Serodiagnosis was performed using enzyme-linked immunosorbent assay (ELISA) with recombinant Ani s 1 and Ani s 7 allergens. Stool samples were examined for soil-transmitted helminths (STHs) using the Kato-Katz method. Structured questionnaires gathered demographic and dietary information.Results: The overall seroprevalence of A. simplex infection was 12.25%. No significant association was found with gender or raw fish consumption. However, parental education and occupation influenced seropositivity. Interestingly, co-infection with STHs was inversely associated with A. simplex seropositivity. ELISA demonstrated high specificity with minimal cross-reactivity to STHs.Conclusions: This study provides the first seroepidemiological data on A. simplex in Nigerian children. Public health interventions, particularly fish-handling education, are warranted. Further research is needed to understand the clinical relevance of A. simplex sensitization in African settings.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An enigmatic case of lepromatous leprosy: unveiling the role of inoculation. 一个谜样的麻风病病例：揭示接种的作用。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-05-24 DOI: 10.1093/trstmh/traf054

Kiranmayee Chandaluri, Sudhir Babu Karri, Vinod Hanumanthu

引用次数: 0

Hydroxynaphthol blue-based loop-mediated isothermal amplification for the rapid detection of Burkholderia pseudomallei. 基于羟基酚蓝的环介导等温扩增快速检测假马氏伯克氏菌。

IF 1.9 4区医学

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-05-23 DOI: 10.1093/trstmh/traf053

Wiphat Klayut, Panatda Aramrueang, Benjawan Phetsuksiri, Sopa Srisungngam, Watcharee Saisongkorh, Ballang Uppapong, Janisara Rudeeaneksin

{"title":"Hydroxynaphthol blue-based loop-mediated isothermal amplification for the rapid detection of Burkholderia pseudomallei.","authors":"Wiphat Klayut, Panatda Aramrueang, Benjawan Phetsuksiri, Sopa Srisungngam, Watcharee Saisongkorh, Ballang Uppapong, Janisara Rudeeaneksin","doi":"10.1093/trstmh/traf053","DOIUrl":"https://doi.org/10.1093/trstmh/traf053","url":null,"abstract":"Background: Melioidosis is a potentially life-threatening disease caused by Burkholderia pseudomallei. It is endemic in Southeast Asia and northern Australia and is increasingly distributed in other tropical and subtropical regions. Fast and accurate detection of B. pseudomallei is crucial for initiating prompt and effective treatment.Methods: Loop-mediated isothermal amplification (LAMP) using hydroxynaphthol blue (HNB) for visual detection has been developed and evaluated to detect B. pseudomallei.Results: The assay amplified B. pseudomallei TTSS1-orf2 at 63°C with a 60-min reaction and no cross-amplification was observed. The detection limit was 1.85×102 fg/µl of B. pseudomallei DNA or 2.08×102 CFU/ml of B. pseudomallei spiked in blood, sputum and urine samples. Clinical evaluation using 191 residual specimens revealed 100% negative agreement in detecting 48 of 49 culture-positive samples. Overall, the sensitivity, specificity, accuracy, positive predictive value and negative predictive value were 97.96%, 100%, 99.48%, 100% and 99.30%, respectively, while the κ index showed substantial agreement with the culture method (κ=0.99).Conclusions: This LAMP assay demonstrated good performance, with easy operation and low cost for the rapid detection of B. pseudomallei. It has the potential to be an alternative molecular tool for the early diagnosis of melioidosis in clinical settings.","PeriodicalId":23218,"journal":{"name":"Transactions of The Royal Society of Tropical Medicine and Hygiene","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0