Orthohantavirus seoulense as a cause of acute, dengue-negative febrile illness in southern Vietnam.

IF 1.9 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Transactions of The Royal Society of Tropical Medicine and Hygiene Pub Date : 2025-04-12 DOI:10.1093/trstmh/traf041

Mai Thi Quynh Le, Kumiko Yoshimatsu, Haruka Abe, Thuy Thi Nguyen, Hang Le Khanh Nguyen, Trang Thi Hong Ung, Phuong Vu Mai Hoang, Nobuo Koizumi, Futoshi Hasebe, Kozue Miura

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引用次数: 0

Abstract

Background: Dengue fever has surged in Vietnam since 2021; however, the aetiology of non-dengue undifferentiated febrile illnesses remains poorly understood.

Methods: Fifty whole blood samples that tested negative in rapid tests for anti-dengue virus antibodies (IgM and IgG) and NS1 viral antigen at Vung Tau General Hospital, southern Vietnam, were subjected to nucleic acid amplification tests for flaviviruses, hantaviruses, Leptospira spp. and Orientia tsutsugamushi, followed by DNA sequencing. The plasma samples were also tested for anti-hantavirus IgM and IgG antibodies using ELISA.

Results: Of the 50 samples, eight were PCR-positive for flaviviruses and two were positive for hantaviruses. Sequencing analysis revealed that three and five of the eight flavivirus-positive samples were dengue virus type 1 and dengue virus type 2, respectively. The hantavirus species was identified as Orthohantavirus seoulense (SEOV). None of the patients tested positive for Leptospira spp. or O. tsutsugamushi. Anti-hantavirus IgM and IgG antibodies were detected in five and four patients, respectively.

Conclusions: This study suggests that SEOV is a notable contributor to dengue-negative febrile illnesses in southern Vietnam.

Accession number: LC822654.

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正汉坦病毒seoulense是越南南部急性登革热阴性发热性疾病的一种病因。

背景：自2021年以来，越南登革热病例激增；然而，非登革热未分化发热性疾病的病因仍然知之甚少。方法：对在越南南部头头总医院抗登革热病毒抗体（IgM和IgG）和NS1病毒抗原快速检测阴性的50份全血标本进行黄病毒、汉坦病毒、钩端螺旋体和恙虫病东方体的核酸扩增检测，并进行DNA测序。采用ELISA法检测血浆样本抗汉坦病毒IgM和IgG抗体。结果：50份样本中，8份黄病毒pcr阳性，2份汉坦病毒pcr阳性。测序结果显示，8份黄病毒阳性样本中3份为1型登革热病毒，5份为2型登革热病毒。汉坦病毒属正汉坦病毒seoulense （SEOV）。所有患者均未检测出钩端螺旋体或恙虫病体阳性。5例和4例患者分别检测到抗汉坦病毒IgM和IgG抗体。结论：本研究表明，SEOV是越南南部登革热阴性发热疾病的显著贡献者。登记号：LC822654。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transactions of The Royal Society of Tropical Medicine and Hygiene 医学-公共卫生、环境卫生与职业卫生

CiteScore

4.00

自引率

9.10%

发文量

115

审稿时长

4-8 weeks

期刊介绍： Transactions of the Royal Society of Tropical Medicine and Hygiene publishes authoritative and impactful original, peer-reviewed articles and reviews on all aspects of tropical medicine.