Tohoku Journal of Experimental Medicine最新文献_第4页

The Clinical Value of LINC01094/Hsa-miR-4758-5p and Their Action Mechanisms in Carotid Artery Stenosis. LINC01094/hsa-miR-4758-5p 在颈动脉狭窄中的临床价值及其作用机制

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-22 Epub Date: 2024-11-07 DOI: 10.1620/tjem.2024.J119

Junwei Xu, Xingxing Wu, Yali Zhang, Ying Jin, Caijiao Wang, Chunchun Xu, Yanfeng Zhang, Li Chen, Haining Zhen

{"title":"The Clinical Value of LINC01094/Hsa-miR-4758-5p and Their Action Mechanisms in Carotid Artery Stenosis.","authors":"Junwei Xu, Xingxing Wu, Yali Zhang, Ying Jin, Caijiao Wang, Chunchun Xu, Yanfeng Zhang, Li Chen, Haining Zhen","doi":"10.1620/tjem.2024.J119","DOIUrl":"10.1620/tjem.2024.J119","url":null,"abstract":"Carotid artery stenosis (CAS) is mostly caused by carotid atherosclerotic plaque, leading to various cardiovascular and cerebrovascular diseases that seriously threaten human lives. This study aims to explore the roles of LINC01094 and hsa-miR-4758-5p in CAS pathogenesis and provide theoretical support for its prediction and treatment. RT-qPCR was used to detect the levels of LINC01094 and hsa-miR-4758-5p. SPSS software was utilized to construct the Receiver Operating Characteristic (ROC) curves for diagnosingCAS using LINC01094 and hsa-miR-4758-5p. The interaction between LINC01094 and hsa-miR-4758-5p was tested using a dual-luciferase reporter gene assay. CCK-8 assay and flow cytometry were employed to assess cell activity and apoptotic cell ratio, respectively. The Western Blotting assay detected the expression of BAK, BCL-2, and ICAM-1. ELISA, lipid peroxidation (MDA), and reactive oxygen species (ROS) kits measured the levels of inflammatory factors (IL-6 and TNF-α), MDA, and ROS, respectively. LINC01094 expressed highly, and hsa-miR-4758-5p expressed lowly in CAS. The combination of LINC01094 and hsa-miR-4758-5p showed higher accuracy in diagnosing CAS compared to either factor alone. The hsa-miR-4758-5p mimic significantly reduced the luciferase activity of HCAECs and THP-1 cells transfected with wt-LINC01094 vectors. LINC01094 overexpression suppressed the level of hsa-miR-4758-5p. The hsa-miR-4758-5p mimic reversed cell damage induced by ox-LDL or LINC01094 overexpression. LINC01094 and hsa-miR-4758-5p had good diagnostic value in CAS. Mechanistically, LINC01094 mediated ox-LDL-induced damage of HCAECs and THP-1 cells by negatively regulating hsa-miR-4758-5p.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"371-379"},"PeriodicalIF":1.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-Difference in Olfactory Interhemispheric Malformation Caused by Pax6 Haploinsufficiency. Pax6单倍不足导致嗅觉半球间畸形的性别差异。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-22 Epub Date: 2025-06-05 DOI: 10.1620/tjem.2025.J068

Natsumi Joko, Takako Kikkawa, Takayoshi Inoue, Noriko Osumi

{"title":"Sex-Difference in Olfactory Interhemispheric Malformation Caused by Pax6 Haploinsufficiency.","authors":"Natsumi Joko, Takako Kikkawa, Takayoshi Inoue, Noriko Osumi","doi":"10.1620/tjem.2025.J068","DOIUrl":"10.1620/tjem.2025.J068","url":null,"abstract":"Sex differences are increasingly recognized as critical factors influencing the onset and manifestation of various neurodevelopmental disorders, including autism spectrum disorder (ASD). Among ASD-associated genes, Pax6, which encodes a transcription factor, has been implicated in sex-dependent phenotypes when mutated. The anterior commissure (AC), a major commissural pathway involved in higher cognitive and social functions, exhibits structural abnormalities both in individuals with ASD and in those carrying Pax6 mutations. Anatomically, the AC consists of two principal fiber bundles: the anterior limb (aAC), connecting the olfactory bulbs and anterior olfactory nucleus, and the posterior limb (pAC), connecting the piriform cortex and amygdaloid regions, both converging at the midline. In this study, we investigated sex-specific structural abnormalities of the AC in Pax6 mutant mice. Analysis of horizontal and sagittal AC sections revealed that Pax6 haploinsufficiency in mice induces abnormal de-fasciculation in the aAC and aberrant intermingling of aAC and pAC fibers at the midline. Notably, Pax6 mutant females exhibited more diverse phenotypes. These females showed a pronounced reduction in overall AC size and disruptions in pAC shape regularity, likely due to a more severe disruption of the boundary between the aAC and pAC axon bundles. These sex-dependent alterations in AC morphology may contribute to the sexually dimorphic phenotypes observed in various neurodevelopmental disorders, including autism spectrum disorder.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"361-369"},"PeriodicalIF":1.6,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144226745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Up-Regulated MiR-616-5p Promotes the Progression of Nasopharynx Cancer through GSK3β. 上调的 MiR-616-5p 通过 GSK3β 促进鼻咽癌的进展

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-21 Epub Date: 2024-11-07 DOI: 10.1620/tjem.2024.J131

Shasha Zhang, Meiai Xu, Xuefeng Shen, Sicong Jiang, Xianming He, Juying Ke

{"title":"Up-Regulated MiR-616-5p Promotes the Progression of Nasopharynx Cancer through GSK3β.","authors":"Shasha Zhang, Meiai Xu, Xuefeng Shen, Sicong Jiang, Xianming He, Juying Ke","doi":"10.1620/tjem.2024.J131","DOIUrl":"10.1620/tjem.2024.J131","url":null,"abstract":"Nasopharyngeal carcinoma (NPC) is a serious malignancy occurring in nasopharynx with high incidence, which is caused by excessive proliferation of nasopharyngeal cells. It has been reported that both microRNA (miR)-616-5p and glycogen synthase kinase 3β (GSK3β) are involved in multiple biological processes in various cancer cells and there is a targeting relationship between them. However, their roles in NPC remain undiscovered. This study was to investigate the prognosis and regulatory role of miR-616-5p/ GSK3β in NPC. 136 patients with NPC were enrolled and provided tissue samples. The NPC cell line SUNE-1 was used to explore the effect of miR-616-5p on NPC. Cell transfection was used to regulate gene expression and the expression levels of miR-616-5p and GSK3β were confirmed by qRT-PCR. Results confirmed that miR-616-5p was increased in both NPC tissues of patients and NPC cell lines. Further Kaplan-Meier assay showed that increased miR-616-5p was related to the poor prognosis of NPC patients. Subsequent assessment on cellular biological behavior demonstrated that up-regulated miR-616-5p facilitated cell proliferation, migration and invasion while it suppressed cell apoptosis. In addition, GSK3β was identified as a potential downstream target of miR-616-5p by dual-luciferase system. In conclusion, up-regulated miR-616-5p may promote the progression of NPC via targeting GSK3β.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"353-360"},"PeriodicalIF":1.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Online with Offline Cognitive-Behavioral Stress Caring Program in Prostate Cancer Survivors after Prostatectomy: A Prospective, Propensity Score-Matched, Cohort Study. 前列腺切除术后前列腺癌幸存者在线与离线认知行为应激护理项目的比较：一项前瞻性、倾向评分匹配的队列研究。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-21 Epub Date: 2025-02-27 DOI: 10.1620/tjem.2025.J030

Hong Qu, Bingbing Song, Yang Lin, Jiao Liu

{"title":"Comparison of Online with Offline Cognitive-Behavioral Stress Caring Program in Prostate Cancer Survivors after Prostatectomy: A Prospective, Propensity Score-Matched, Cohort Study.","authors":"Hong Qu, Bingbing Song, Yang Lin, Jiao Liu","doi":"10.1620/tjem.2025.J030","DOIUrl":"10.1620/tjem.2025.J030","url":null,"abstract":"Offline cognitive-behavioral stress caring program (CBSC) modifies maladaptive cognition and improves management stress skills, which relieves mental disorders in some cancer patients; however, it is limited by space and time. This study compared the effect of online and offline CBSC on mental health, spiritual well-being, fatigue, and patient satisfaction in prostate cancer survivors after prostatectomy. Totally, 147 prostate cancer survivors after prostatectomy were divided into offline (n = 89) and online (n = 58) CBSC groups. CBSC included stress management, relaxation, disease-relevant education, and weekly homework. Selection bias was adjusted by matching patients in both groups (1:1) using propensity scores (n = 58 in both groups after adjustment). Hospital Anxiety and Depression Scale (HADS)-anxiety, HADS-depression, Functional Assessment of Chronic Illness Therapy Spiritual Well-Being Scale (FACIT-SP), the brief fatigue inventory-China (BFI-C), and patient satisfaction score (PSS) were evaluated. 'Improvement' was defined as positive improvement values in indexes after a 10-week CBSC. Before and after adjustment, HADS-anxiety, HADS-depression, FACIT-SP, BFI-severity, and BFI-interference scores were improved at week 10 vs. baseline in the online or offline CBSC group. However, these scores did not vary at baseline or week 10 between groups. The improvement of HADS-anxiety, HADS-depression, and FACIT-SP scores was smaller in online CBSC group than in offline CBSC group. The improvement of BFI-severity and BFI-interference scores were non-differential between groups. PSS scores were reduced in online CBSC group vs. offline CBSC group. Overall, online CBSC alleviates mental disorders, elevates satisfaction, and relieves fatigue in prostate cancer survivors after prostatectomy, but its overall effect is less than offline CBSC.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"343-351"},"PeriodicalIF":1.6,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Traditional Japanese Samurai Movement Rei-ho as a Knee Extension Strength Training: A Randomized Controlled Study. 日本传统武士动作“礼”作为膝关节伸展力量训练：一项随机对照研究。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-14 DOI: 10.1620/tjem.2025.J099

Ayaka Ogasawara, Akira Sato, Ryoichi Nagatomi

引用次数: 0

Epidural Anesthesia Using Mixed Reality Technology: A Successful Case Report of Spinal Visualization in a Patient with Difficult Epidural Anesthesia. 硬膜外麻醉应用混合现实技术：硬膜外麻醉困难患者脊柱显像的成功案例报告。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-14 DOI: 10.1620/tjem.2025.J098

Shun Nagashima, Tatsuya Hayasaka, Kazunori Sato, Akira Takagi, Makiko Kikuhara, Rintaro Tsunoda, Yuta Komoriya, Koutaro Honda, Yu Onodera, Masafumi Kanoto, Kaneyuki Kawamae, Hiroaki Toyama

引用次数: 0

Blood Resolvin D1 is Associated with a Lower Acute Myocardial Infarction Risk. 血液分辨率D1与较低的急性心肌梗死风险相关

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-14 DOI: 10.1620/tjem.2025.J100

Junfeng Song, Jing Zhang, Qiang Feng, Yangxia Huang, Yanni Huang, Yunzhi Lian

引用次数: 0

Hounsfield Unit Comparison of Opposite Hemispheres in Computed Tomography for Early Diagnosis of Ischemic Stroke. 早期诊断缺血性脑卒中计算机断层扫描对脑半球的Hounsfield单位比较。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-08 Epub Date: 2025-02-13 DOI: 10.1620/tjem.2025.J017

Selcuk Coskun, Serkan Demircan, Gul Pamukcu Gunaydin, Karabekir Ercan

{"title":"Hounsfield Unit Comparison of Opposite Hemispheres in Computed Tomography for Early Diagnosis of Ischemic Stroke.","authors":"Selcuk Coskun, Serkan Demircan, Gul Pamukcu Gunaydin, Karabekir Ercan","doi":"10.1620/tjem.2025.J017","DOIUrl":"10.1620/tjem.2025.J017","url":null,"abstract":"Sometimes, non-contrast computed tomography (NCCT) is the only neuroimaging technique available due to the disadvantages of magnetic resonance imaging (MRI) (e.g., MRI is not suitable for patients with a cardiac pacemaker, hemodynamically unstable patients etc.). This study aims to examine the use of Hounsfield Unit (HU) measurement comparison of symmetrical anatomical regions in opposite hemispheres of the brain for the early diagnosis of acute ischemic stroke patients. Eighty-one ischemic stroke patients were included in this retrospective study. Infarct areas were defined in diffusion-weighted magnetic resonance imaging (DWMRI) as hyperintense areas. The same anatomical regions were then identified in previously performed computed tomography, and the density of these areas in HUs was measured. Symmetrical anatomical regions were found in the opposite hemisphere, and HU measurements of these areas were obtained. The means of infarct area HU measurements and normal area HU measurements were compared between groups. The infarct area HU measurements (IAHUm) were significantly lower than the symmetrical normal area HU measurements (NAHUm) (p < 0.001) in all patient groups. The ratio of infarct to normal area HU measurements (rHU) showed remarkable consistency across groups (0.75, 0.77, 0.68). Our findings suggest that comparing HU measurements is an effective means of early diagnosis for ischemic stroke. We propose that this technique may be useful for patients when DWMRI is not available or not suitable. We further suggest that this technique can be used more accurately if the calculations are done automatically by computer software.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"305-309"},"PeriodicalIF":1.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143410867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Phosphodiesterase-3 Inhibitor in the Management of Left Heart Failure in Extremely Low-Birth-Weight Infants with Simple Aortic Coarctation. 磷酸二酯酶-3抑制剂在极低出生体重儿单侧主动脉缩窄左心衰治疗中的作用

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-08 Epub Date: 2025-03-06 DOI: 10.1620/tjem.2025.J034

Hideyuki Hawaka, Katsuaki Toyoshima, Tomoko Saito, Tomoyuki Shimokaze, Kazuma Tsuno, Takeshi Ikegawa, Junko Nakajima, Atsushi Uchiyama

{"title":"Effects of Phosphodiesterase-3 Inhibitor in the Management of Left Heart Failure in Extremely Low-Birth-Weight Infants with Simple Aortic Coarctation.","authors":"Hideyuki Hawaka, Katsuaki Toyoshima, Tomoko Saito, Tomoyuki Shimokaze, Kazuma Tsuno, Takeshi Ikegawa, Junko Nakajima, Atsushi Uchiyama","doi":"10.1620/tjem.2025.J034","DOIUrl":"10.1620/tjem.2025.J034","url":null,"abstract":"We herein present a case of simple aortic coarctation (CoA) after ductus arteriosus ligation, in which the combination of a phosphodiesterase-3 (PDE3) inhibitor with liposomal prostaglandin E1 (lipoPGE1) improved the pressure gradient between the upper and lower limbs and enhanced left ventricular function. A female infant was born at 29 weeks 1 day of gestation as the smaller twin in a monochorionic diamniotic pregnancy, with a birth weight of 744 g (-3.0 SD). She was diagnosed with simple CoA at 2 days of age and treated with lipoPGE1. However, because of progressive congestive heart failure, patent ductus arteriosus ligation was performed at 9 days of age. By 21 days of age, the pressure gradient between her upper and lower limbs worsened to 55 mmHg despite continued lipoPGE1 treatment. At that point, the PDE3 inhibitor olprinone was introduced in combination with lipoPGE1. After starting the combination therapy, the pressure gradient decreased to 24 mmHg while the blood pressure in the lower extremities remained similar, and the left ventricular function improved. The PDE3 inhibitor may have contributed to improving the stenosis of the CoA site. Further studies are needed to clarify the efficacy of this treatment.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"319-326"},"PeriodicalIF":1.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploratory Study on the Relationship between Head and Neck Cancer and Japanese Cedar Pollinosis. 头颈癌与杉木授粉病关系的探索性研究。

IF 1.6 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2025-08-08 Epub Date: 2024-12-12 DOI: 10.1620/tjem.2024.J142

Shigeyuki Murono, Sayaka Yagi-Nakanishi, Kazuhira Endo, Satoru Kondo, Hisashi Sugimoto, Tomokazu Yoshizaki

{"title":"Exploratory Study on the Relationship between Head and Neck Cancer and Japanese Cedar Pollinosis.","authors":"Shigeyuki Murono, Sayaka Yagi-Nakanishi, Kazuhira Endo, Satoru Kondo, Hisashi Sugimoto, Tomokazu Yoshizaki","doi":"10.1620/tjem.2024.J142","DOIUrl":"10.1620/tjem.2024.J142","url":null,"abstract":"Allergic rhinitis, especially Japanese cedar pollinosis, is considered a national affliction in Japan. The present exploratory study focused on the AllergoOncologic aspect of head and neck cancer (HNC) from the perspective of serum allergen-specific IgE against Japanese cedar pollen (JCP) for the first time. Serum JCP-specific IgE was measured in seventy-five HNC patients, including the oropharynx, hypopharynx, and larynx, and seventeen nasopharyngeal cancer (NPC) patients as well as forty-two patients with non-allergic benign disease (nABD). The JCP-specific IgE-positive rate was significantly lower in HNC than nABD (p = 0.037). Similarly, there was a clear trend toward a lower JCP-specific IgE score in HNC than nABD (p = 0.069). Further analyses excluding subjects in their 70s or older also showed that the JCP-specific IgE-positive rate was significantly lower in HNC than nABD (p = 0.022). In addition, The JCP-specific IgE score was significantly lower in HNC than nABD (p = 0.045). A lower serum JCP-specific IgE-positive rate in patients with HNC compared with nABD was demonstrated for the first time. An inverse correlation between HNC and Japanese cedar pollinosis was suggested from the perspective of allergen-specific IgE.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"335-342"},"PeriodicalIF":1.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0