Tohoku Journal of Experimental Medicine最新文献

{"title":"Long Noncoding RNA ST8SIA6-AS1 Promotes the Development of Prostate Cancer by Activating the TGF-β/Smad Signaling Pathway Through Regulating the miR-18a-5p/TGFBR1 Axis.","authors":"Qingshan Liu, Jian Ke, Yu Luo","doi":"10.1620/tjem.2025.J140","DOIUrl":"10.1620/tjem.2025.J140","url":null,"abstract":"<p><p>Prostate cancer (PCa) is one of the most malignant tumors in men. ST8SIA6-AS1 is a newly identified lncRNA that has been identified as an oncogene in many human cancers. We aimed to investigate the biological effects of lncRNA ST8SIA6-AS1 on the development of PCa. We found that ST8SIA6-AS1 expression was elevated in PCa tissues and cells. ST8SIA6-AS1 knockdown inhibited cell proliferation and migration and promoted cell apoptosis in PCa cells. Furthermore, ST8SIA6-AS1 bound with miR-18a-5p in PCa. TGFBR1 was directly targeted by miR-18a-5p in PCa and mediated the biological influence of miR-18a-5p and ST8SIA6-AS1 in PCa, resulting in TGF-β/Smad signaling pathway activation. Importantly, ST8SIA6-AS1 silencing suppressed tumor growth in PCa xenograft mouse models. In summary, this paper first demonstrated that ST8SIA6-AS1 played an oncogenic role via sponging miR-18a-5p and promoting TGFBR1 level and TGF-β/Smad activation in PCa, providing novel insights for the therapeutic strategies of PCa.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"527-539"},"PeriodicalIF":1.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146019280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KRAS and MET Co-Mutation in One Lesion and EGFR Mutation in Another Lesion of Early-Stage Synchronous Multiple Lung Adenocarcinomas: A Case Report.","authors":"Takayasu Ito, Ken Onodera, Naoya Ishida, Sakiko Kumata, Takeo Togo, Tatsuaki Watanabe, Yui Watanabe, Takashi Hirama, Takaya Suzuki, Hirotsugu Notsuda, Hisashi Oishi, Yoshinori Okada","doi":"10.1620/tjem.2025.J157","DOIUrl":"10.1620/tjem.2025.J157","url":null,"abstract":"<p><p>Recent advances in medical technology have led to an increased number of reports of various driver mutations and cases of synchronous multiple primary lung cancer. Although multiple driver mutations in a lung cancer lesion are generally considered mutually exclusive, recent studies have reported rare cases of coexisting mutations known as co-mutations. In this case report, we describe a rare case of three synchronous lung adenocarcinomas presenting as ground-glass opacities in the left upper lung lobe of a 68-year-old man. The patient underwent a thoracoscopic left upper division segmentectomy. Pathological examination of these lesions revealed early-stage lung adenocarcinomas with varying degrees of invasiveness. Genetic testing revealed a MET exon 14 skipping mutation and a KRAS mutation in one lesion, and an EGFR L858R mutation in another. The postoperative course was uneventful, and no recurrence has been detected since 1 year and 9 months after surgery (without adjunctive treatment). This case is notable because co-mutations were present in one lesion, while a different driver mutation was present in another ground-glass nodule. To the best of our knowledge, only one case of synchronous multiple primary lung cancer harboring a co-mutation has been reported to date. The findings of this case report suggest that multiple driver mutations and even co-mutations can arise in the early stages of carcinogenesis. The accumulation of similar cases in the future is expected to help elucidate the molecular mechanisms and clinical significance of these mutations.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"541-545"},"PeriodicalIF":1.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145828412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence for Breast Carcinoma Detection in Histopathological Images Based on Single Shot MultiBox Detector in Intraoperative Rapid Diagnosis.","authors":"Mio Yamaguchi-Tanaka, Tomoaki Sasaki, Kodai Uemura, Yuichiro Tajima, Sho Kato, Takayoshi Asakura, Kiyoshi Takagi, Yuto Yamazaki, Atsushi Masamune, Toshio Miyata, Takashi Suzuki","doi":"10.1620/tjem.2025.J120","DOIUrl":"10.1620/tjem.2025.J120","url":null,"abstract":"<p><p>Intraoperative rapid diagnosis with pathological classification by pathologists plays a pivotal role in making appropriate surgical decisions. However, morphological evaluation of intraoperative frozen sections is apparently more difficult than that of formalin fixed paraffin embedded (FFPE) tissue because of their unavoidable tissue-processing artifacts, which compels pathologists, even well-trained, more challenging to make an accurate diagnosis. Therefore, assisting the pathological diagnosis with artificial intelligence is anticipated. Here, we built the data set consisting of histopathological micrographs of intraoperative frozen sections and demonstrated an automatic breast carcinoma detection model based on a Single Shot MultiBox Detector, an object detection method known for real-time, high-speed performance. The final 3-class classification model was trained on 943 intraoperative frozen section images and evaluated using 65 images, identifying benign lesions, non-invasive carcinoma, and invasive carcinoma. The 2-class (benign or malignant) and 3-class classification tasks achieved diagnostic accuracies for whole image of 92.3% and 89.2%, respectively. When we evaluated the performance of object detection in the 2-class task, the precision, recall and F1 scores for malignant detection are 70.3%, 74.4%, and 72.3%, respectively. Additionally, the model's object detection performance was assessed using FFPE images, yielding a precision of 55.1%, a sensitivity of 76.9%, and an F1 score of 64.2%, suggesting that it is particularly well-suited for intraoperative frozen sections. In conclusion, we presented the automatic breast carcinoma detection method, utilizing histopathological micrographs of intraoperative frozen sections. The present model may assist pathologists by reducing their workload and minimizing the risk of overlooking carcinoma cells in intraoperative diagnosis.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"507-516"},"PeriodicalIF":1.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145669577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corynoxine Attenuates Proliferation and Epithelial-Mesenchymal Transition in Triple-Negative Breast Cancer Cells Through Inactivation of the JAK2/STAT3/PIM1 Signaling Cascade.","authors":"Meijuan Lan, Daoxu Huang, Mengchun Xiao, Lingmin Chi","doi":"10.1620/tjem.2026.J103","DOIUrl":"https://doi.org/10.1620/tjem.2026.J103","url":null,"abstract":"","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Management of Aspiration Pneumonia in Older Adults with Frailty.","authors":"Satoru Ebihara","doi":"10.1620/tjem.2026.J110","DOIUrl":"https://doi.org/10.1620/tjem.2026.J110","url":null,"abstract":"","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"METTL1-Enhanced SMC4 mRNA Stability via m⁷G Modification Promotes Hepatocellular Carcinoma Progression and Confers the 5-Fluorouracil Resistance.","authors":"Tang Dan, He Zhi, Yang Xiuzhi, Ao Yu, Liu Gongwei, Wu Qiang, Chen Jingxiang","doi":"10.1620/tjem.2026.J107","DOIUrl":"https://doi.org/10.1620/tjem.2026.J107","url":null,"abstract":"","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long Intergenic Non-Protein Coding RNA 1355 Drives Hepatocellular Carcinoma Progression by Promoting Epithelial-Mesenchymal Transition and M2 Macrophage Polarization.","authors":"Jie Zhang, Guanbo Zhang, Zhi Yang, Lin Yang, Gang Li, Jinsong Li, Jiaxing Wang","doi":"10.1620/tjem.2026.J101","DOIUrl":"https://doi.org/10.1620/tjem.2026.J101","url":null,"abstract":"","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":""},"PeriodicalIF":1.5,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149115271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Neighborhood Built Environments with Physical Activity and Diabetes Mellitus Incidence.","authors":"Yasuyuki Kondo, Kei Hamazaki, Chiho Yamazaki, Satoshi Tsukui, Naoya Yahagi, Hiroshi Koyama","doi":"10.1620/tjem.2026.J039","DOIUrl":"10.1620/tjem.2026.J039","url":null,"abstract":"<p><p>Globally, increasing evidence suggests that neighborhood built environments (NBE)-including walkability, access to green spaces, and food outlets-can shape residents' physical activity and lifestyle behaviors, with implications for chronic disease risk. Although several studies suggest that walkable and activity-supportive environments promote better health, other findings indicate that certain environmental features, including food establishments, might increase obesity and diabetes mellitus (DM). How different environmental elements affect both physical activity and DM incidence remains unclear. We investigated associations of NBE with physical activity and DM incidence. This study included 87,154 individuals (33,884 males and 53,270 females) with national health insurance in Gunma Prefecture, Japan, who underwent the Specific Health Checkup in FY2011. These individuals were followed up for 7 years (until FY2018). The odds ratio (OR) for engaging in physical activity was significantly higher in neighborhoods with convenience stores [OR: 1.07, 95% confidence interval (CI): 1.03, 1.10] or parks (OR: 1.09, 95% CI: 1.06, 1.13) but significantly lower in neighborhoods with train stations (OR: 0.96, 95% CI: 0.92, 0.99). The proportion of individuals engaging in physical activity tended to increase when the number of NBE increased. DM incidence risk was significantly lower in neighborhoods with parks [hazard ratio (HR): 0.90, 95% CI: 0.83, 0.98] and higher in neighborhoods with convenience stores (HR: 1.09, 95% CI: 1.01, 1.18). These results suggest that having parks in neighborhoods may contribute to reducing DM risk, whereas convenience stores may increase it. Future urban design policies should consider both protective and risk-enhancing neighborhood features.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"517-525"},"PeriodicalIF":1.5,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147594671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aberrant Expression of lncRNA TTC28-AS1/miR-767-5p in Allergic Rhinitis and Its Potential Effect on the Disease Progression.","authors":"Dan Kan, Long Ran, Yongming Yu, Yuyong Huang, Guangming Lou, Junchun Wu, Yang Pan","doi":"10.1620/tjem.2025.J134","DOIUrl":"10.1620/tjem.2025.J134","url":null,"abstract":"<p><p>Allergic rhinitis (AR) is a prevalent chronic inflammatory disease with significant global morbidity, severely impacting patients' quality of life and potentially leading to multiple complications. Long non-coding RNAs (lncRNAs) play critical roles in immune regulation and inflammatory responses, but their specific mechanisms in AR remain unclear. This study aimed to investigate the expression profile, clinical relevance, and functional mechanism of lncRNA TTC28-AS1 in AR, focusing on its regulation of nasal epithelial inflammation via miR-767-5p. RT-qPCR was used to measure lnc TTC28-AS1, miR-767-5p, and inflammatory cytokines (GM-CSF, eotaxin-1) expression in nasal mucosa tissues from AR patients and healthy controls. Receiver operating characteristic (ROC) curve analysis evaluated the diagnostic efficacy of lnc TTC28-AS1, while Pearson correlation assessed its association with clinical symptom scores. The targeting relationship between lnc TTC28-AS1 and miR-767-5p was validated by dual-luciferase reporter assay. In an interleukin (IL)-13-induced human nasal epithelial cell (HNEC) model, the effects of the lnc TTC28-AS1/miR-767-5p axis on cell proliferation were analyzed via CCK-8 assay. Apoptosis was examined by flow cytometry analysis. Lnc TTC28-AS1 was significantly upregulated in AR patients and positively correlated with total nasal symptom score (TNSS). Its overexpression was an independent AR risk factor. Lnc TTC28-AS1 directly suppressed miR-767-5p, exacerbating inflammation and apoptosis in HNECs. Silencing lnc TTC28-AS1 reduced IL-13-induced GM-CSF/eotaxin-1 expression and apoptosis promoting proliferation, effects reversed by miR-767-5p inhibition. In conclusion, Lnc TTC28-AS1 modulates inflammatory responses and functional status of nasal epithelial cells by targeting miR-767-5p, suggesting its potential as a novel diagnostic biomarker and therapeutic target for AR.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"499-506"},"PeriodicalIF":1.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Diagnostic Value of miR-142-3p in Pediatric Acute Respiratory Distress Syndromes and Its Effects on Human Lung Epithelial Cells.","authors":"Wenjing Yan, Jianzhu He, Yangping Zhou, Jian Zhu","doi":"10.1620/tjem.2025.J142","DOIUrl":"10.1620/tjem.2025.J142","url":null,"abstract":"<p><p>Pediatric acute respiratory distress syndrome (PARDS) is a major cause of morbidity and mortality in critically ill children. microRNAs (miRNAs) were discovered to be involved in regulating the progression of numerous disorders, including PARDS. We aim to explore the diagnostic value and relevant mechanisms of miR-142-3p in PARDS. Real-time quantitative PCR (RT-qPCR) was used to detect the levels of miR-142-3p and myristoylated-alanine rich C-kinase substrate (MARCKS) in the serum of PARDS patients or in A549 cells. Receiver operating characteristic (ROC) curve analysis predicted the diagnostic value of miR-142-3p for PARDS. The proliferation, apoptosis and inflammatory factor concentrations in lipopolysaccharide (LPS)-induced A549 cells were measured using the Cell Counting Kit-8 (CCK-8) assay, flow cytometry and Enzyme-Linked Immunosorbent Assay (ELISA), respectively. We found that the content of miR-142-3p was markedly elevated in PARDS group and positively correlated with disease severity. It had good diagnostic ability for PARDS. Moreover, miR-142-3p and pro-inflammatory factors were remarkably positively correlated. In vitro, a cell model was constructed by inducing A549 cells with LPS. Cell proliferation was repressed while apoptosis and inflammation were facilitated in LPS-induced A549 cells. Up-regulated miR-142-3p enhanced apoptosis and inflammatory factor secretion while suppressed proliferation. Down-regulated miR-142-3p facilitated proliferation while repressed apoptosis and inflammatory factor secretion. Furthermore, MARCKS was a target gene of miR-142-3p and down-regulated in cell model. miR-142-3p may be a novel diagnostic marker for PARDS. In LPS-induced A549 cells, miR-142-3p repressed cell proliferation and facilitated apoptosis and inflammatory responses via modulating MARCKS.</p>","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":" ","pages":"471-477"},"PeriodicalIF":1.5,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145985827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}