Tohoku Journal of Experimental Medicine最新文献_第10页

Circular RNA DLGAP4 Inhibits Ischemic Stroke-Induced Microglia M1 Polarization and Proinflammatory Cytokine Production, Possibly through the NF-κB Pathway. 环形 RNA DLGAP4 可通过 NF-κB 通路抑制缺血性中风诱导的小胶质细胞 M1 极化和促炎细胞因子的产生

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-30 DOI: 10.1620/tjem.2024.J036

Ji Ding, Yun Zhang, Min Xu

引用次数: 0

Comparative Gene-Expression Analysis of the Ligamentum Flavum of Patients with Lumbar Spinal Canal Stenosis: Comparison between the Dural and Dorsal Sides of the Thickened Ligamentum Flavum. 腰椎管狭窄症患者黄韧带的基因表达比较分析：增厚韧带硬膜侧和背侧的比较

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-25 Epub Date: 2024-02-15 DOI: 10.1620/tjem.2024.J015

Yutaka Yabe, Taro Takemura, Shinya Hattori, Keisuke Ishikawa, Toshimi Aizawa

{"title":"Comparative Gene-Expression Analysis of the Ligamentum Flavum of Patients with Lumbar Spinal Canal Stenosis: Comparison between the Dural and Dorsal Sides of the Thickened Ligamentum Flavum.","authors":"Yutaka Yabe, Taro Takemura, Shinya Hattori, Keisuke Ishikawa, Toshimi Aizawa","doi":"10.1620/tjem.2024.J015","DOIUrl":"10.1620/tjem.2024.J015","url":null,"abstract":"Thickening of the ligamentum flavum is the main factor in the development of lumbar spinal canal stenosis (LSCS). Although previous studies have reported factors related to ligamentum flavum thickening, its etiology has not been clarified. Furthermore, it is often difficult to set proper controls to investigate the pathologies of thickening due to differences in patient characteristics, such as age, sex, obesity, and comorbidities. This study aimed to elucidate the pathologies of ligamentum flavum thickening by comparing the dural and dorsal sides of the thickened ligamentum flavum in patients with LSCS. Ligamentum flavum samples were collected from 19 patients with LSCS. The samples were divided into the dural and dorsal sides. The dural side was used as a control to assess the pathologies occurring on the dorsal side. Elastic Masson staining was used to assess the elastic fibres. Gene expression levels were comprehensively assessed using quantitative reverse transcription polymerase chain reaction and DNA microarray analyses. Gene ontology analysis was used to identify biological processes associated with differentially expressed genes. The elastic fibres were significantly decreased on the dorsal side of the thickened ligamentum flavum. Genes related to fibrosis, inflammation, tissue repair, remodeling, and chondrometaplasia, such as COL1A2, COL3A1, COL5A1, TGFB1, VEGFA, TNFA, MMP2, COL10A1, and ADAMTS4, were highly expressed on the dorsal side of the thickened ligamentum flavum. The biological processes occurring on the dorsal side of the thickened ligamentum flavum were extracellular matrix organization, cell adhesion, extracellular matrix disassembly, and proteolysis.These are considered important pathologies of ligamentum flavum thickening.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Predictors of Primary Lung Cancer among Part-Solid Ground-Grass Nodules on High-Resolution CT. 高分辨率 CT 上部分实性草地结节中原发性肺癌的形态学预测因素

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-25 Epub Date: 2024-02-15 DOI: 10.1620/tjem.2024.J016

Hirotsugu Notsuda, Hiroki Oshio, Ken Onodera, Takashi Hirama, Yui Watanabe, Tatsuaki Watanabe, Takaya Suzuki, Hisashi Oishi, Hiromichi Niikawa, Ryoko Saito-Koyama, Masafumi Noda, Junya Tominaga, Yoshinori Okada

{"title":"Morphological Predictors of Primary Lung Cancer among Part-Solid Ground-Grass Nodules on High-Resolution CT.","authors":"Hirotsugu Notsuda, Hiroki Oshio, Ken Onodera, Takashi Hirama, Yui Watanabe, Tatsuaki Watanabe, Takaya Suzuki, Hisashi Oishi, Hiromichi Niikawa, Ryoko Saito-Koyama, Masafumi Noda, Junya Tominaga, Yoshinori Okada","doi":"10.1620/tjem.2024.J016","DOIUrl":"10.1620/tjem.2024.J016","url":null,"abstract":"Recent advancements in computed tomography (CT) scanning have improved the detection rates of peripheral pulmonary nodules, including those with ground-glass opacities (GGOs). This study focuses on part-solid pure ground-glass nodules (GGNs) and aims to identify imaging predictors that can reliably differentiate primary lung cancer from nodules with other diagnoses among part-solid GGNs on high-resolution CT (HRCT). A retrospective study was conducted on 609 patients who underwent surgical treatment or observation for lung nodules. Radiological findings from pre-operative HRCT scans were reviewed and several CT imaging features of part-solid GGNs were examined for their positive predictive value to identify primary lung cancer. The proportions of the nodules with a final diagnosis of primary lung cancer were significantly higher in part-solid GGNs (91.9%) compared with solid nodules (70.3%) or pure GGNs (66.7%). Among CT imaging features of part-solid GGNs that were evaluated, consolidation-to-tumor ratio (CTR) < 0.5 (98.1%), pleural indentation (96.4%), and clear tumor border (96.7%) had high positive predictive value to identify primary lung cancer. When two imaging features were combined, the combination of CTR < 0.5 and a clear tumor border was identified to have 100% positive predictive values with a sensitivity of 40.8%. Thus we conclude that part-solid GGNs with a CTR < 0.5 accompanied by a clear tumor border evaluated by HRCT are very likely to be primary lung cancers with an acceptable sensitivity. Preoperative diagnostic procedures to obtain a pathological diagnosis may potentially be omitted in patients harboring such part-solid GGNs.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of CD4⁺T Cells in Myeloproliferative Diseases and the Effect of Ruxolitinib Treatment on Prognosis. 骨髓增生性疾病中 CD4+T 细胞的表达及 Ruxolitinib 治疗对预后的影响

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-24 DOI: 10.1620/tjem.2024.J029

Xiaoying Song, Yiping Yang, Jingjing Cui, Jing Sun, Jun Zhang, Lichang Gao, Jianqiang Liu

引用次数: 0

Ultrasound-Guided Transversus Abdominis Plane Block versus Quadratus Lumborum Block for Postoperative Analgesia after Cesarean Section Delivery. 超声引导下腹横肌平面阻滞与腰四肌阻滞用于剖宫产术后镇痛的比较

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-24 DOI: 10.1620/tjem.2024.J032

Osama Allam Mandour, Doaa Mohamed Almonayery Brahim, Mostafa Mohamed Elsayed, Mahmoud Mostafa Elshamy, Amira Sobhy Eldeeb, Nashwa Mohammed Ibrahiem, Suzan Mohamed Abdelkareem, Mayada Yahia Elbohouty, Hend Mostafa Abosaifa, Hanaa A M Khalil, Gihan Eissa Zahran, Amira Aboelnaser Awad, Mohamed Abdelmonem Donya, Abeer Shaban Goda

引用次数: 0

Effectiveness of Roy Adaptation Model-Based Cognitive Stimulation Therapy in Elderly Patients with Non-Small Cell Lung Cancer Undergoing Curative Resection. 基于罗伊适应模型的认知刺激疗法对接受根治性切除术的非小细胞肺癌老年患者的疗效

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-24 Epub Date: 2024-01-12 DOI: 10.1620/tjem.2023.J108

Cong-Ying Chen, Hui Ding, Shang-Shang Wang

{"title":"Effectiveness of Roy Adaptation Model-Based Cognitive Stimulation Therapy in Elderly Patients with Non-Small Cell Lung Cancer Undergoing Curative Resection.","authors":"Cong-Ying Chen, Hui Ding, Shang-Shang Wang","doi":"10.1620/tjem.2023.J108","DOIUrl":"10.1620/tjem.2023.J108","url":null,"abstract":"This study aimed to investigate the effects of a Roy adaptation model (RAM)-based cognitive stimulation therapy (CST) intervention on elderly patients diagnosed with primary non-small cell lung cancer (NSCLC) undergoing curative resection. A total of 280 patients diagnosed with primary NSCLC were randomized into RAM-based CST group and control group. Outcomes were assessed at three intervals: pre-surgery, discharge, and one-month post-discharge. Cognitive function was evaluated using Mini-Cognitive test. Postoperative delirium prevalence was determined within 48 hours post-surgery using Nursing Delirium Screening Scale. The Hospital Anxiety and Depression Scale evaluated anxiety and depression symptoms, while Quality of Life (QoL) was assessed via Short Form-36 (SF36) Health Survey. The RAM-based CST group demonstrated significantly higher Mini-Cog test scores than the control group upon discharge and post-intervention. Patients with RAM-based CST exhibited a decrease in postoperative delirium compared to the control group. The RAM-based CST intervention yielded an improvement in anxiety and depression at discharge and 1-month post-discharge compared to preoperative levels. Additionally, the RAM-based CST group exhibited substantial enhancements in SF36 subcategory scores at 1-month post-discharge compared to pre-surgery. At post-intervention, the RAM-based CST group demonstrated significantly higher scores than the control group across various health-related domains, including role limitations due to emotional problems, mental health, general health perception, bodily pain, and role limitations due to physical problems. The RAM-based CST intervention in elderly NSCLC patients undergoing curative resection yielded significant enhancements in cognitive function, reduced delirium incidence, improved emotional well-being, and better QoL postoperatively.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of Pleural Solitary Fibrous Tumors Causing Severe Hypoglycemia after Exceptionally Long Asymptomatic Periods: Report of Two Surgical Cases. 胸膜单发纤维瘤在超长无症状期后导致严重低血糖的演变：两个手术病例的报告。

IF 1.7 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-23 Epub Date: 2024-02-08 DOI: 10.1620/tjem.2024.J012

Takaya Suzuki, Hirotsugu Notsuda, Hisashi Oishi, Hiromichi Niikawa, Tatsuaki Watanabe, Yui Watanabe, Ken Onodera, Tetsuto Takeda, Ringo Sugawara, Masafumi Noda, Kanako Sakurai, Mototsugu Nagao, Izumi Fukuda, Yoshinori Okada

{"title":"Evolution of Pleural Solitary Fibrous Tumors Causing Severe Hypoglycemia after Exceptionally Long Asymptomatic Periods: Report of Two Surgical Cases.","authors":"Takaya Suzuki, Hirotsugu Notsuda, Hisashi Oishi, Hiromichi Niikawa, Tatsuaki Watanabe, Yui Watanabe, Ken Onodera, Tetsuto Takeda, Ringo Sugawara, Masafumi Noda, Kanako Sakurai, Mototsugu Nagao, Izumi Fukuda, Yoshinori Okada","doi":"10.1620/tjem.2024.J012","DOIUrl":"10.1620/tjem.2024.J012","url":null,"abstract":"Non-islet cell tumor hypoglycemia (NICTH) is one of the paraneoplastic syndromes manifesting severe hypoglycemia caused by aberrant production of high-molecular-weight insulin-like growth factor 2 (big-IGF2). Two surgical cases of extremely large thoracic solitary fibrous tumors (SFT) with unusual history of NICTH are presented. One case manifested severe hypoglycemia after four years of the first complete surgical resection of the tumor with potential malignant transformation, and the other case showed severe hypoglycemia after ten years of the first detection of the tumor. Meticulous laboratory testing, including serum endocrinological tests and western immunoblotting before and after surgery was performed, and both cases were diagnosed as NICTH. Both patients underwent open thoracic surgery. The patients showed normal glucose and hormone levels immediately after the resection of responsible tumors with elevated blood insulin concentration. SFTs are generally considered benign; however, life-threatening hypoglycemia can happen regardless of treatment. Careful follow-up of the tumor growth is warranted.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":1.7,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-Term and Short-Interval Assessment of Self-Reported Urinary and Sexual Functions after Nerve-Sparing Radical Hysterectomy: A Prospective Cohort Study. 神经根治性子宫切除术后泌尿和性功能自我报告的长期和短期评估：前瞻性队列研究

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-22 Epub Date: 2024-02-08 DOI: 10.1620/tjem.2024.J014

Masumi Ishibashi, Hitoshi Niikura, Motoko Ishida, Noriyuki Iwama, Hirohito Metoki, Shogo Shigeta, Tomoyuki Nagai, Hideki Tokunaga, Muneaki Shimada, Nobuo Yaegashi

{"title":"Long-Term and Short-Interval Assessment of Self-Reported Urinary and Sexual Functions after Nerve-Sparing Radical Hysterectomy: A Prospective Cohort Study.","authors":"Masumi Ishibashi, Hitoshi Niikura, Motoko Ishida, Noriyuki Iwama, Hirohito Metoki, Shogo Shigeta, Tomoyuki Nagai, Hideki Tokunaga, Muneaki Shimada, Nobuo Yaegashi","doi":"10.1620/tjem.2024.J014","DOIUrl":"10.1620/tjem.2024.J014","url":null,"abstract":"The aim of this study was to determine the impact of nerve preservation confirmed by intraoperative electrical stimulation (IES) on subjective symptoms of urinary and sexual function in uterine cervical cancer patients who underwent radical hysterectomies. This study included 85 patients who underwent type C radical hysterectomy with IES. Pelvic splanchnic nerve preservation with IES after hysterectomy (nerve-stimulation positive group) was confirmed in 61 women and 24 women did not have nerve preservation (negative group). Urinary function was assessed with the Overactive Bladder Symptom Score (OABSS), International Prostate Symptom Score (IPSS), and International Consultation on Incontinence Questionnaire-Short Form (ICIQ-SF) questionnaires. Sexual function was surveyed using the Female Sexual Function Index (FSFI). Longitudinal changes in those scores according to response to nerve-stimulation were evaluated using a generalized estimating equation. IPSS quality of life (QOL) scores were significantly better in the nerve-stimulation positive group compared with the scores in the negative group until 12 months after surgery, whereas OABSS, IPSS total, IPSS voiding, and ICIQ-SF scores evaluating urinary symptoms were not significantly different between the two groups. FSFI scores were better in the nerve-stimulation positive group 36 months after surgery compared with the scores in the negative group. In this study, we assessed self-reported urinary and sexual symptoms after nerve-sparing radical hysterectomy (NSRH) with IES in the long term. We demonstrated that nerve-sparing significantly reduced distress associated with QOL until 1 year, improved urinary storage symptoms at 2 years, and sexual symptoms 3 years after surgery.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AXIN1/MYC Axis Mediated the Osimertinib Resistance in EGFR Mutant Non-Small Cell Lung Cancer Cells. AXIN1/MYC轴介导表皮生长因子受体突变非小细胞肺癌细胞对奥希替尼的耐药性

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-01 Epub Date: 2024-01-18 DOI: 10.1620/tjem.2024.J002

Haoyue Yu, Zhiguo Wang, Yan Dong, Li Li, Xianming Fan, Nan Zheng, Ji Jiang, Caiyu Lin, Conghua Lu, Kunlin Li, Mingxia Feng

{"title":"AXIN1/MYC Axis Mediated the Osimertinib Resistance in EGFR Mutant Non-Small Cell Lung Cancer Cells.","authors":"Haoyue Yu, Zhiguo Wang, Yan Dong, Li Li, Xianming Fan, Nan Zheng, Ji Jiang, Caiyu Lin, Conghua Lu, Kunlin Li, Mingxia Feng","doi":"10.1620/tjem.2024.J002","DOIUrl":"10.1620/tjem.2024.J002","url":null,"abstract":"Osimertinib, a promising and approved third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), is a standard strategy for EGFR-mutant non-small cell lung cancer (NSCLC) patients. However, developed resistance is unavoidable, which reduces its long-term effectiveness. In this study, RNA sequencing was performed to analyze differentially expressed genes (DEGs). The PrognoScan database and Gene Expression Profiling Interactive Analysis (GEPIA) were used to identify the key genes for clinical prognosis and gene correlation respectively. Protein expression was determined by western blot analysis. Cell viability assay and Ki67 staining were used to evaluate the effect of osimertinib on tumor cells. Finally, we screened out two hub genes, myelocytomatosis oncogene (Myc) and axis inhibition protein 1 (Axin1), upregulated in three osimertinib-resistant cell lines through RNA sequencing and bioinformatics analysis. Next, cell experiment confirmed that expression of C-MYC and AXIN1 were elevated in different EGFR mutant NSCLC cell lines with acquired resistance to osimertinib, compared with their corresponding parental cell lines. Furthermore, we demonstrated that AXIN1 upregulated the expression of C-MYC and mediated the acquired resistance of EGFR mutant NSCLC cells to osimertinib in vitro. In conclusion, AXIN1 affected the sensitivity of EGFR mutant NSCLC to osimertinib via regulating C-MYC expression in vitro. Targeting AXIN1/MYC signaling may be a potential new strategy for overcoming acquired resistance to osimertinib.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139485876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anamorelin Induced Acute Hyperglycemia in a Patient with Advanced Pancreatic Cancer and Diabetes: A Case Report. 阿那莫瑞林诱发晚期胰腺癌合并糖尿病患者急性高血糖：病例报告。

IF 2.2 4区医学

Tohoku Journal of Experimental Medicine Pub Date : 2024-05-01 Epub Date: 2024-02-08 DOI: 10.1620/tjem.2024.J013

Junpei Yamamoto, Hiroshi Onodera, Yuki Kaminaga, Yoko Kayaba, Masahiro Usui

{"title":"Anamorelin Induced Acute Hyperglycemia in a Patient with Advanced Pancreatic Cancer and Diabetes: A Case Report.","authors":"Junpei Yamamoto, Hiroshi Onodera, Yuki Kaminaga, Yoko Kayaba, Masahiro Usui","doi":"10.1620/tjem.2024.J013","DOIUrl":"10.1620/tjem.2024.J013","url":null,"abstract":"Anamorelin (ANAM) is a novel ghrelin receptor agonist for the treatment of cancer cachexia. In clinical trials of ANAM, glucose metabolism disorders as adverse effects were relatively frequent, however, when and how they occur remains unclear. Moreover, the safety in patients with pancreatic cancer and/or diabetes has not been clarified because most previous studies focused on patients with non-small cell lung cancer and had excluded patients with poorly controlled diabetes. Herein, a 66-year-old man with advanced pancreatic cancer and diabetes was administered ANAM, and acute hyperglycemia was developed and could be monitored by the self-monitoring of blood glucose (SMBG). Increasing the insulin dose failed to control hyperglycemia adequately, but the hyperglycemia ameliorated quickly after ANAM discontinuation. The continuous glucose monitoring (CGM) revealed that the sensor glucose levels had remained in the high range throughout the day during ANAM administration despite using 1.5 times more insulin. Our report is one of the few that describe the details of ANAM-induced hyperglycemia and provides important information for the safe and effective use of ANAM.","PeriodicalId":23187,"journal":{"name":"Tohoku Journal of Experimental Medicine","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139703519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0