Cornuside Ameliorates Diabetic Nephropathy Possibly by Regulating Angiogenesis and MAPK Signaling.

IF 1.7 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Tohoku Journal of Experimental Medicine Pub Date : 2025-05-28 Epub Date: 2024-10-24 DOI:10.1620/tjem.2024.J112

Fang Xiang, Xin Li, Wei Hu

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引用次数: 0

Abstract

Diabetic nephropathy (DN) is a prevalent diabetic complication seriously threatening patients' health and lives. Cornuside is an iridoid glycoside compound with various pharmacological properties. Nonetheless, whether cornuside has a protective effect against DN remains unillustrated. In this study, a rat model for DN was established by streptozotocin (STZ) injection. Rat podocytes were stimulated with high glucose (HG) to mimic a DN microenvironment in vitro. Several indicators linked to kidney function were evaluated. Periodic Acid-Schiff (PAS) and hematoxylin-eosin staining were implemented for renal histologic analysis. Immunofluorescence staining of CD31 was used for the detection of neovascularization. Western blotting was employed to assess levels of angiogenic factors and MAPK signaling-related proteins in the kidney or podocytes. The results showed that cornuside administration alleviated STZ-elicited renal dysfunction, as evidenced by the reduction in fasting blood glucose, proteinuria, serum creatinine, and blood urea nitrogen. Cornuside attenuated renal pathological lesions in DN rats. Cornuside repressed angiogenesis in DN rat kidney tissues and podocytes. Cornuside blocked MAPK signaling in HG-stimulated podocytes. In conclusion, cornuside alleviates renal injury in DN rats possibly by hindering angiogenesis and MAPK signaling.

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山茱萸苷可能通过调节血管生成和 MAPK 信号转导改善糖尿病肾病

糖尿病肾病（DN）是糖尿病常见的并发症，严重威胁患者的健康和生命。角苷是一种环烯醚萜苷类化合物，具有多种药理特性。尽管如此，玉米酰胺是否对DN有保护作用仍未得到证实。本研究采用链脲佐菌素（STZ）注射建立DN大鼠模型。用高糖（HG）刺激大鼠足细胞模拟体外DN微环境。评估了与肾功能相关的几个指标。采用周期性酸-希夫（PAS）和苏木精-伊红染色进行肾脏组织学分析。采用CD31免疫荧光染色检测新生血管。Western blotting用于评估肾脏或足细胞中血管生成因子和MAPK信号相关蛋白的水平。结果显示，山茱萸可减轻stz引起的肾功能障碍，其表现为空腹血糖、蛋白尿、血清肌酐和血尿素氮的降低。角苷减轻DN大鼠肾脏病理损害。角苷抑制DN大鼠肾组织和足细胞血管生成。Cornuside阻断hg刺激足细胞中的MAPK信号。综上所述，山茱萸苷可能通过抑制血管生成和MAPK信号通路来减轻DN大鼠的肾损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tohoku Journal of Experimental Medicine 医学-医学：内科

CiteScore

3.60

自引率

4.50%

发文量

171

审稿时长

1 months

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