Toxicology and Industrial Health最新文献_第3页

Reproductive toxicity of endosulfan: Mechanisms and impacts on female and male reproductive health. 硫丹的生殖毒性：机制及其对女性和男性生殖健康的影响。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2026-01-01 Epub Date: 2026-01-18 DOI: 10.1177/07482337261417731

Elizabeth Glanet Durom, V A Aneesha, T S Shyamkumar, Ajmi Bin Azeez, C S Celus, Ayushi Vaidhya, Subhashree Parida, Madhu C Lingaraju, Thakur Uttam Singh, Dinesh Kumar

{"title":"Reproductive toxicity of endosulfan: Mechanisms and impacts on female and male reproductive health.","authors":"Elizabeth Glanet Durom, V A Aneesha, T S Shyamkumar, Ajmi Bin Azeez, C S Celus, Ayushi Vaidhya, Subhashree Parida, Madhu C Lingaraju, Thakur Uttam Singh, Dinesh Kumar","doi":"10.1177/07482337261417731","DOIUrl":"10.1177/07482337261417731","url":null,"abstract":"Endosulfan is a broad-spectrum organochlorine pesticide widely used in many developing countries despite its high toxicity potential. Endosulfan, listed as potent endocrine-disrupting chemical and xenoestrogen, gains importance for its potential to cause reproductive and developmental dysfunction. In females, endosulfan disrupts ovarian and uterine development, leading to infertility, miscarriage, and developmental toxicity. It acts by mimicking estrogen and interferes with estrogen and androgen pathways, impacting hormone regulation and gene expression, including estrogen receptor α (ERα) and progesterone receptors. Endosulfan triggers oxidative stress in ovaries, reduces follicle count, and impairs uterine differentiation, affecting embryo implantation. Additionally, it alters gene expression and causes epigenetic modifications, contributing to reproductive dysfunctions. In males, endosulfan affects spermatogenesis by causing oxidative stress, mitochondrial dysfunction, and lipid peroxidation. It reduces sperm quality, motility, and quantity, with effects on testicular tissues, sperm chromatin condensation, and enzymatic activity. Oxidative damage, increased reactive oxygen species (ROS), and disrupted energy metabolism are central to its toxicity. Epidemiological studies also link pesticide exposure to reduced sperm counts, higher DNA fragmentation, and infertility. Moreover, endosulfan can cross the placental barrier, leading to fetal resorption, malformations, and maternal toxicity. This review provides a comprehensive overview of the reproductive toxicity of endosulfan in males and females. We also highlight the various possible mechanisms of reproductive toxicity of endosulfan and its potential to impart deleterious effects over HPG axis, gonads, and uterine differentiation and development and implantation.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"39-56"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic effects of Aloe vera against X-ray irradiation-induced structural alterations and DNA damage in mice. 芦荟对x射线照射引起的小鼠结构改变和DNA损伤的预防作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2026-01-01 Epub Date: 2025-12-01 DOI: 10.1177/07482337251400982

Shashi Bala, Lalit Kumar Gupta, Ashwani Koul

{"title":"Prophylactic effects of Aloe vera against X-ray irradiation-induced structural alterations and DNA damage in mice.","authors":"Shashi Bala, Lalit Kumar Gupta, Ashwani Koul","doi":"10.1177/07482337251400982","DOIUrl":"10.1177/07482337251400982","url":null,"abstract":"Radiation exposures, whether planned or unexpected, can have harmful consequences on biological systems. To avoid these radiotoxic consequences, a variety of natural and synthetic radioprotectors are given prior to radiation exposure. The present study aimed to investigate the radioprotective potential of Aloe vera gel extract against damage induced by whole-body X-ray exposure in male balb/c mice. The animals were divided into four groups: Control, Aloe vera (50 mg/kg body weight orally on alternate days for 30 days), X-ray (cumulative dose of 2 Gy) (i.e., 0.258 Gy twice a day for four consecutive days) in the last week of the experimental protocol, and Aloe vera + X-ray. Following irradiation, Fourier transform infrared spectroscopy (FTIR) analysis showed structural changes in molecules by altering the ratios of CH3/lipid, CH2/lipid, lipid/protein, Amide I/II, and nucleic acid in liver and testes. X-ray exposure led to an increase in DNA strand breaks in the liver, spleen, kidney, and testes of mice, as indicated by comet assay. Bcl-2 levels were shown to be up-regulated in the kidney and testes following irradiation. In liver, kidney, and spleen, caspase 3 was also found to be up-regulated. Furthermore, after being exposed to X-rays, the expression of caspase 9 and bax was up-regulated in all the tissues examined. Administration of Aloe vera to the X-ray-exposed group exhibited decline in DNA strand breaks in all tissues investigated. Moreover, protein expressions of these genes were found to be down-regulated in the Aloe vera + X-ray-exposed group. These observations illustrate the potential of Aloe vera in providing radioprotection for rodents, possibly DNA protection, through its anti-apoptotic properties.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"11-28"},"PeriodicalIF":1.7,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145655728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hidden harm? Trade secret designations and transparency in consumer product labeling. 隐藏的伤害?商业秘密指定和消费品标签的透明度。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2026-01-01 Epub Date: 2025-11-18 DOI: 10.1177/07482337251397791

Peter G Kreysa

引用次数: 0

Acute ethyl acetate exposure suppresses spontaneous locomotor activity and exploration behaviour and impairs reference memory performance in rats: No evidence to support a role for L-type calcium channels. 急性乙酸乙酯暴露抑制大鼠自发运动活动和探索行为，损害参考记忆表现：没有证据支持l型钙通道的作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-12-01 Epub Date: 2025-11-17 DOI: 10.1177/07482337251394072

Pınar Yamantürk-Çelik, Selçuk Şen, Zeynep Güneş Özünal

{"title":"Acute ethyl acetate exposure suppresses spontaneous locomotor activity and exploration behaviour and impairs reference memory performance in rats: No evidence to support a role for L-type calcium channels.","authors":"Pınar Yamantürk-Çelik, Selçuk Şen, Zeynep Güneş Özünal","doi":"10.1177/07482337251394072","DOIUrl":"10.1177/07482337251394072","url":null,"abstract":"Occupational exposure to volatile organic solvents has been reported to lead to hazardous effects. Ethyl acetate is a volatile organic compound used commonly in industry and found in many commercial products. The present study aimed to investigate the acute behavioural effects of ethyl acetate exposure in rats. The mechanism of its effects was further investigated by focusing on the possible involvement of L-type calcium channels. For this purpose, ethyl acetate (0.3 g/kg, i.p.) alone or concurrently with nimodipine (3 and 5 mg/kg, i.p.), a dihydropyridine calcium channel antagonist selective to L-type calcium channels, was administered to male Wistar albino rats. When compared to the saline control group, ethyl acetate significantly decreased the number of square-crossing, rearing, and sniffing in the open-field and impaired the reference memory performance in the three-panel runway. However, administration of nimodipine at the given doses did not block these effects of ethyl acetate. The findings suggest that L-type calcium channels may not contribute to the mechanism(s) responsible for the acute toxicity of ethyl acetate in rats regarding their central nervous system depression and memory disturbances although it should be more thoroughly investigated in further studies.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"523-530"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective effect of ursodeoxycholic acid in a co-exposure model of cadmium-induced kidney damage. 熊去氧胆酸对镉致肾损伤共暴露模型的保护作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-12-01 Epub Date: 2025-11-22 DOI: 10.1177/07482337251392671

Kemal Kavakli, Durmus Hatipoglu, Aysegul Bulut, Mehmet Burak Ates

{"title":"Protective effect of ursodeoxycholic acid in a co-exposure model of cadmium-induced kidney damage.","authors":"Kemal Kavakli, Durmus Hatipoglu, Aysegul Bulut, Mehmet Burak Ates","doi":"10.1177/07482337251392671","DOIUrl":"10.1177/07482337251392671","url":null,"abstract":"Cadmium (Cd) is a toxic environmental heavy metal with a long biological half-life and a high affinity for renal accumulation, posing serious health risks in both humans and animals. Ursodeoxycholic acid (UDCA), a bile acid primarily used for liver disorders, has gained attention for its antioxidant properties and potential protective effects in various toxicological settings. This study investigated the protective role of UDCA against cadmium-induced renal injury in rats. Twenty-eight male Wistar Albino rats (180-220 g) were divided into four groups: control (C, n = 6), cadmium (Cd, n = 8), UDCA (n = 6), and cadmium + UDCA (Cd + UDCA, n = 8). Cd was administered intraperitoneally (3 mg/kg) from days 8 to 15. UDCA was given intragastrically (60 mg/kg) from days 1 to 15. The Cd + UDCA group received both treatments concurrently. Biochemical analysis showed that Cd exposure significantly elevated serum BUN, creatinine, KIM-1, and MDA levels while decreasing GSH concentrations, indicating oxidative stress and renal injury. Conversely, the Cd + UDCA group demonstrated marked attenuation of these changes, with significantly lower BUN, creatinine, KIM-1, and MDA levels, and partially preserved GSH levels. Histopathological examination confirmed extensive renal damage in the Cd group, including cortical necrosis, tubular debris, vascular damage, glomerular alterations, and interstitial inflammation. These alterations were less severe in the Cd + UDCA group, supporting the biochemical findings. In conclusion, UDCA partially mitigated cadmium-induced oxidative stress and nephrotoxicity, reducing renal injury and supporting its potential as a protective agent against heavy metal-induced kidney damage.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"531-542"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential protection of taurine against potassium bromate- induced oxidative damage in human erythrocytes. 牛磺酸对溴酸钾诱导的人红细胞氧化损伤的潜在保护作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI: 10.1177/07482337251385294

Saima Nazir, Mir Kaisar Ahmad, Zubair-Ul-Nazir

{"title":"Potential protection of taurine against potassium bromate- induced oxidative damage in human erythrocytes.","authors":"Saima Nazir, Mir Kaisar Ahmad, Zubair-Ul-Nazir","doi":"10.1177/07482337251385294","DOIUrl":"10.1177/07482337251385294","url":null,"abstract":"Potassium bromate (KBrO3), a strong oxidizing agent, is widely used in various industrial settings, thereby increasing the risk of oxidative damage and subsequent tissue toxicity in individuals who are exposed to it. Additionally, KBrO3 has been used as a maturing agent and is also generated as a by-product during water disinfection process. Previously, we have demonstrated that KBrO3 causes oxidative damage to human erythrocytes. The present work shows the protective effects of taurine (2-aminoethane sulfonic acid) against oxidative stress induced by KBrO3 in human erythrocytes. Erythrocytes from healthy donors were incubated with either KBrO3 alone or taurine alone and KBrO3 plus varying concentrations of taurine. The treatment of erythrocytes with KBrO3 caused disruption in the oxidative-reductive homeostasis as evidenced by severe alterations in antioxidant enzyme functions and a marked depletion in total sulfhydryl content. Exposure of erythrocytes to KBrO3 also caused the elevation in methemoglobin levels, protein carbonyls, hydrogen peroxide levels, protein oxidation, and lipid peroxidation. However, the KBrO3-induced cellular/biochemical alterations were greatly protected by taurine. These results suggest that taurine significantly decreases the toxic effects of KBrO3 in human erythrocytes.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"563-574"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145356266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Health risk assessment of occupational exposure to hydrogen peroxide (H₂O₂) among dairy processing plant workers. 乳制品加工厂工人职业接触过氧化氢（H2O2）的健康风险评估。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1177/07482337251392506

Somayeh Rahimimoghadam, Danial Soleymani-Ghoozhdi, Safoura Javan, Mahdi Jalali

{"title":"Health risk assessment of occupational exposure to hydrogen peroxide (H2O2) among dairy processing plant workers.","authors":"Somayeh Rahimimoghadam, Danial Soleymani-Ghoozhdi, Safoura Javan, Mahdi Jalali","doi":"10.1177/07482337251392506","DOIUrl":"10.1177/07482337251392506","url":null,"abstract":"Hydrogen peroxide (H2O2) is a well-known irritant that affects the eyes, respiratory tract, and skin, and long-term exposure may lead to chronic respiratory conditions such as bronchitis, pulmonary fibrosis, and an increased risk of lung cancer, particularly in occupational settings. The objective of this study was to evaluate occupational exposure to H2O2 among dairy processing plant workers and to characterize the associated semi-quantitative risks using the methodology of the Singapore Occupational Health Department. This research was conducted in the sterilization units of three dairy processing plants located in Neyshabur, Iran. The semi-quantitative risk assessment in this study adhered to the methodology recommended by the Singapore Occupational Health Department. The findings revealed that the average occupational exposure to H2O2 for 29 workers across three dairy processing plants was 0.91 ± 0.54 ppm, with individual exposure levels ranging from 0.10 to 2.12 ppm. The mean exposure level exceeded the adjusted threshold limit value (TLV) for H2O2 (0.78 ppm). The evaluation of health risks associated with H2O2 exposure revealed that all participants fell within the medium-risk category. This classification underscores the potential for adverse health effects among dairy workers and highlights the need for targeted interventions to mitigate exposure risks and enhance workplace safety.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"543-549"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145496878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The insecticide chlorpyrifos leads to genotoxic effects by triggering oxidative stress and apoptosis in Sertoli cells. 杀虫剂毒死蜱通过触发支持细胞的氧化应激和凋亡而引起遗传毒性作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-12-01 Epub Date: 2025-11-17 DOI: 10.1177/07482337251387921

Yasemin Aydin, Hatice Kurtel, Emine Cakir, Banu Orta Yilmaz

{"title":"The insecticide chlorpyrifos leads to genotoxic effects by triggering oxidative stress and apoptosis in Sertoli cells.","authors":"Yasemin Aydin, Hatice Kurtel, Emine Cakir, Banu Orta Yilmaz","doi":"10.1177/07482337251387921","DOIUrl":"10.1177/07482337251387921","url":null,"abstract":"Chlorpyrifos (CPF) is an insecticide that humans are exposed to when used for agricultural and domestic purposes. Many studies have shown that CPF has adverse effects on human health and causes toxicity in various cells and tissues. Since there are few studies in the literature examining the effects of CPF on the male reproductive system, Sertoli cells, which are crucial to the continuation of spermatogenesis, were chosen as a model in our study. The objective of this study was to investigate the cytotoxic, oxidative stress-related, apoptotic, and genotoxic effects of chlorpyrifos in TM4 Sertoli cells. In this study, Sertoli cells were exposed to two concentrations of CPF (100 and 500 μM) for 24 h. The cytotoxic and genotoxic potential of CPF in Sertoli cells was evaluated by measuring metabolic activity, malondialdehyde content, antioxidant enzyme levels, apoptosis rates, and DNA damage. According to the findings, applied CPF concentrations increased oxidative stress and decreased antioxidant enzyme levels in Sertoli cells. The results indicated that cell viability and the nuclear division index decreased in a concentration-dependent manner, while apoptotic cells, the micronucleus, and comet formation increased. Consequently, the results suggest that DNA damage and apoptosis play a major role in the mechanisms underlying the cytotoxic effects of CPF in Sertoli cells, establishing CPF as a strong genotoxic agent.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"550-562"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145542682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of DNA damage in long-term paint exposed workers by non-invasive buccal micronucleus assay. 用非侵入性口腔微核测定法评价长期接触油漆工人的DNA损伤。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-10-01 Epub Date: 2025-10-16 DOI: 10.1177/07482337251383382

İzem Bilinmiş, Senanur Durna, Sultan Pınar Çetintepe, Sevtap Aydin Dilsiz, Nurşen Başaran

{"title":"Evaluation of DNA damage in long-term paint exposed workers by non-invasive buccal micronucleus assay.","authors":"İzem Bilinmiş, Senanur Durna, Sultan Pınar Çetintepe, Sevtap Aydin Dilsiz, Nurşen Başaran","doi":"10.1177/07482337251383382","DOIUrl":"10.1177/07482337251383382","url":null,"abstract":"Workers involved in paint production or application are extensively exposed to various hazardous substances like organic solvents, lead-based pigments, adhesives, and residual plastic monomers. Therefore, workers in the paint industry are at high risk of suffering adverse health effects. Studies of the lymphocytes of paint workers have demonstrated that industrial paint induces DNA damage and cellular changes. The aim of the present study was to assess DNA damage in 54 paint workers from paint production and application areas and 54 age-gender matched control subjects using a non-invasive buccal micronucleus (MN) assay. Buccal MN frequencies were significantly increased in workers compared to controls. MN frequencies significantly increased among workers from paint production areas compared with workers from paint application areas. MN frequencies in long-term workers (>10 years) were found to be significantly higher than those of short-term workers (≤10 years), which indicates that the duration of exposure to paints causes cytogenetic damage. MN frequencies increased with increasing age, while smoking status and the use of protective masks had no additional effect on MN frequencies within groups. In conclusion, it appears that long-term exposure to complex chemical mixtures during paint production may increase DNA damage in the workers. Understanding the possible causes of occupational exposure-induced genotoxicity in paint industry workers is of great importance for the protection of public health. Monitoring variables related to genotoxic damage in the paint workers using non-invasive methods will facilitate and improve risk assessment in the paint production sector.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"473-486"},"PeriodicalIF":1.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145309405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined toxicity of nine typical pesticides in binary mixtures on A549 cells. 九种典型农药二元混合物对A549细胞的联合毒性研究。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-10-01 Epub Date: 2025-09-05 DOI: 10.1177/07482337251374930

Weizhang Qi, Tingting Meng, Shujie Wang, Chang Chen, Zhiyong Cao, Ke Fang, Yuying Liu, Yanhong Shi, Jinjing Xiao

{"title":"Combined toxicity of nine typical pesticides in binary mixtures on A549 cells.","authors":"Weizhang Qi, Tingting Meng, Shujie Wang, Chang Chen, Zhiyong Cao, Ke Fang, Yuying Liu, Yanhong Shi, Jinjing Xiao","doi":"10.1177/07482337251374930","DOIUrl":"10.1177/07482337251374930","url":null,"abstract":"Air pollution caused by pesticide drift poses a significant environmental health risk. The lungs are directly targeted by airborne pesticide exposure via inhalation; however, their inhalation toxicological data are poorly understood. In the present study, we evaluated the combined toxicity and interactions of lambda-cyhalothrin and its binary mixtures with eight insecticides at a concentration ratio of 1:1 in the non-small-cell lung cancer A549 line cells. Cytotoxicity tests showed that the selected pesticide binary mixtures reduced cell viability in a concentration-dependent manner. The combination index (CI) model indicated that the mixtures of lambda-cyhalothrin with acetamiprid, thiamethoxam, or clothianidin exhibited antagonistic effects, while other combinations shifted from antagonism to synergy at a toxicity level of 40%-60%. A synergistic toxicity occurred between lambda-cyhalothrin and emamectin benzoate with CI values of 0.58-0.95, and the obtained benchmark dose limits (BMDLs) value of their binary mixture was greater than 1.5 times more toxic than that of these pesticides individually. Moreover, combined exposure to the binary mixtures produced greater amounts (1.30- to 2.93-fold increase) of reactive oxygen species, malondialdehyde, and lactate dehydrogenase compared with exposure to these pesticides individually. This result suggested that the synergistic cytotoxicity of lambda-cyhalothrin and emamectin benzoate may be driven by excessive oxidative stress. Our findings serve to better understand the complex toxic pesticide interactions that humans face in the environment.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"497-507"},"PeriodicalIF":1.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0