Toxicology and Industrial Health最新文献_第2页

Virtual screening study for biological activity assessment and metabolism pathway of a fuel dye in airborne exposure scenario. 燃料染料在空气暴露情况下的生物活性评估和代谢途径的虚拟筛选研究。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-01-01 Epub Date: 2024-09-23 DOI: 10.1177/07482337241286187

Sayed Vahid Esmaeili, Ali Alboghobeish, Vafa Feyzi, Fatemeh Ravannakhjavani, Rezvan Zendehdel

{"title":"Virtual screening study for biological activity assessment and metabolism pathway of a fuel dye in airborne exposure scenario.","authors":"Sayed Vahid Esmaeili, Ali Alboghobeish, Vafa Feyzi, Fatemeh Ravannakhjavani, Rezvan Zendehdel","doi":"10.1177/07482337241286187","DOIUrl":"10.1177/07482337241286187","url":null,"abstract":"The utilization of synthetic dyes increases the risk to human health. Despite the progress of information on azo dyes, very little attention has been reported on toxicity assessment of anthraquinone dyes. Solvent Blue 35 (SB35) is one of the anthraquinone dyes likely to be encountered because of its increasing use in various industries. Whereas the design of laboratory tests is very expensive, in silico screening was used to predict the metabolic profile and toxicity effect of SB35. MetaTox software was used to predict the metabolites of phase I and II in two layers. Since airborne exposure has been considered, the pathways of inhalation and dermal absorption of SB35 were investigated through the SwissADME model based on the modified Lipinski's rule of five. To predict the biological effect and toxicity of SB35 and each of the metabolites, PASS online software was used. Chemical activity was considered according to the probability of activation values (Pa) higher than the probability of inactivation values (Pi). N- dealkylation of SB35 was predicted in the first layer, while seven active compounds were obtained in the second layer from phases I and II reactions. Investigating the physicochemical properties of SB35 confirmed inhalation absorption for occupational exposure scenarios. All metabolites are absorbed from intestinal routes based on the RO5 rules. SB35 and their metabolites have an effective substrate role for the sub-type of CYP 450 enzymes. The toxicity effect of carcinogenicity for SB35 and mutagenicity for metabolites are predicted while confirmed with some biological effects. However, reproductive disorders are pointed with SB35 by probability higher than 70%. Virtual screening methods are efficient tools for creating cost-effective predictions in the hazard's evaluation of SB35. However, a perspective view is suggested before decision-making for laboratory designing tests.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142308612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case series and clinical analysis of acute hydrogen sulfide poisoning: Experience from 10 cases at a hospital in Zhoushan.

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-20 DOI: 10.1177/07482337241308388

Yuechuan Shen, Guangfen Zhao, Jingkai Lin, Junyan Wang, Bin Luo, Jingye Liu, Yini Zhang, Junhua Huang

{"title":"Case series and clinical analysis of acute hydrogen sulfide poisoning: Experience from 10 cases at a hospital in Zhoushan.","authors":"Yuechuan Shen, Guangfen Zhao, Jingkai Lin, Junyan Wang, Bin Luo, Jingye Liu, Yini Zhang, Junhua Huang","doi":"10.1177/07482337241308388","DOIUrl":"https://doi.org/10.1177/07482337241308388","url":null,"abstract":"This study investigated the etiology, clinical features, and management of acute hydrogen sulfide (H2S) poisoning in Zhoushan. A retrospective analysis was conducted on 10 patients admitted to our hospital between August and September 2023 due to two incidents of acute H2S poisoning. The first incident involved fishermen working in a fishing cabin (6 patients), while the second involved sanitation workers during sewer maintenance (4 patients). Among the patients, 4 had severe poisoning, 3 had moderate poisoning, and 3 had mild poisoning. Corneal chemical injuries were observed in 4 severe patients, and chest CT scans showed bilateral infiltrative changes in 7 patients. Elevated lactate concentrations, and low oxygenation indices were noted in all severe patients. Severe cases received intensive care, including tracheal intubation, mechanical ventilation, corticosteroids, methylene blue, ulinastatin, and hyperbaric oxygen therapy. Patients with mild to moderate symptoms received supportive treatments, including oxygen therapy and hyperbaric oxygen therapy. With the exception of one fatality, all other patients were discharged after successful treatment. Fishing boat cabins and decomposed sewage channels in island areas are common sites for acute H2S poisoning. Rapid identification of H2S poisoning and evaluation are crucial. Early airway management is essential for severe cases to prevent vital organ hypoxia.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"7482337241308388"},"PeriodicalIF":1.7,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142865506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Manganese exposure and sleep quality in iron and steel industry workers. 钢铁工业工人的锰暴露与睡眠质量

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-18 DOI: 10.1177/07482337241302885

Somayeh Rahimimoghadam, Reza Pourbabaki, Fatemeh Khorashadizadeh, Arash Mohammadi, Mojtaba Emkani

{"title":"Manganese exposure and sleep quality in iron and steel industry workers.","authors":"Somayeh Rahimimoghadam, Reza Pourbabaki, Fatemeh Khorashadizadeh, Arash Mohammadi, Mojtaba Emkani","doi":"10.1177/07482337241302885","DOIUrl":"https://doi.org/10.1177/07482337241302885","url":null,"abstract":"Manganese (Mn) is an essential element crucial for the proper functioning of the human body. However, excessive exposure to manganese can lead to complications, particularly neurotoxicity. Among the health issues associated with exposure to heavy metals, one of the major concerns in the adverse effect on sleep quality. A total of 189 employees from a steel factory were divided into two groups: exposed (149 people) and non-exposed (40 people). Air samples were collected using the NIOSH 7300 method, and blood samples were obtained at the end of each shift. The samples underwent analysis by ICP-OES after preparation using the acid-thermal digestion method. To gather information on sleep quality, the Petersburg Sleep Questionnaire (PSQI) was used. The data collected in this study showed abnormal conditions, leading to the inclusion of medians alongside averages. The participants had an average age of 35 and an average work experience of 6 years. The exposed group had a significantly higher median respiratory exposure to manganese (1.32 mg/m3) compared with the non-exposed group (0.20 mg/m3). The average sleep quality score in the exposed group was significantly worse (score of 7) compared with the non-exposed group (score of 4). In addition, there was a significant relationship between the quality of sleep and the level of manganese in the air, so that the quality of sleep decreases with the increase in the level of manganese in the air (p-value = .005). However, no significant relationship was observed between blood manganese level and air manganese level in the exposed group (p-value = .06).","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"7482337241302885"},"PeriodicalIF":1.7,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142847987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of Ferroptosis and NF-κB/NLRP3/MAPK Pathways in Methylmercury-Induced Hepatotoxicity.

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-13 DOI: 10.1177/07482337241307067

Yueqing Xie, Hongsen Yu, Yingrong Ye, Jingjing Wang, Zhengtao Yang, Ershun Zhou

{"title":"Activation of Ferroptosis and NF-κB/NLRP3/MAPK Pathways in Methylmercury-Induced Hepatotoxicity.","authors":"Yueqing Xie, Hongsen Yu, Yingrong Ye, Jingjing Wang, Zhengtao Yang, Ershun Zhou","doi":"10.1177/07482337241307067","DOIUrl":"https://doi.org/10.1177/07482337241307067","url":null,"abstract":"Methylmercury (MeHg) is a potent hepatotoxin with a complex mechanism of inducing liver injury. Ferroptosis, an iron-dependent form of non-apoptotic cell death, is implicated in various toxicological responses, but its role in MeHg-induced liver damage remains under investigation. In this study, we established an acute liver injury (ALI) model in mice via gavage of MeHg (0, 40, 80, 160 μmol/kg). Histopathological analysis revealed dose-dependent liver damage, corroborated by elevated serum biochemical markers, confirming MeHg-induced hepatotoxicity. MeHg exposure raised MDA levels, inhibited SOD and GSH activity, and downregulated CAT expression. Increased iron accumulation and elevated transferrin receptor expression were observed, alongside decreased GPX4 and SLC7A11 levels, indicating ferroptosis involvement. Additionally, inflammation in MeHg-exposed livers was markedly intensified, as evidenced by increased MPO activity, upregulation of pro-inflammatory cytokines, and activation of the NF-κB/NLRP3 signaling pathway. The Keap1/NRF2/HO-1 oxidative stress response pathway was significantly activated, and p38/ERK1/2 MAPK signaling was notably increased. These findings suggested that MeHg induced acute liver injury through the interplay of ferroptosis, oxidative stress, inflammation, and MAPK signaling pathways, providing a scientific basis for future exploration of the mechanisms underlying MeHg-induced hepatotoxicity and potential therapeutic strategies.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"7482337241307067"},"PeriodicalIF":1.7,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of xylene exposure during organogenesis on foeto-placental efficiency and foetal viability: Exploring its association with oxidative stress-induced inflammation and apoptosis in utero. 器官形成过程中接触二甲苯对胎盘效率和胎儿存活率的影响：探索二甲苯与氧化应激诱导的子宫内炎症和细胞凋亡的关系

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI: 10.1177/07482337241286569

Noor Asyikin Suaidi, Mohammed Abdullah Alshawsh, See-Ziau Hoe, Mohd Helmy Mokhtar, Siti Rosmani Md Zin

{"title":"Impact of xylene exposure during organogenesis on foeto-placental efficiency and foetal viability: Exploring its association with oxidative stress-induced inflammation and apoptosis in utero.","authors":"Noor Asyikin Suaidi, Mohammed Abdullah Alshawsh, See-Ziau Hoe, Mohd Helmy Mokhtar, Siti Rosmani Md Zin","doi":"10.1177/07482337241286569","DOIUrl":"10.1177/07482337241286569","url":null,"abstract":"The potential maternal and foetal toxicity resulting from exposure to xylene at or below the allowable limit of 100 ppm during gestation is not thoroughly studied. The aim of this study was to investigate maternal and foetal outcomes following prenatal exposure to xylene during organogenesis. Pregnant Sprague Dawley (SD) rats were administered intraperitoneal (IP) corn oil (vehicle), 100, 500, and 1000 parts per million (ppm) of xylene from gestational day (GD) 6 until GD17. Clinical signs, maternal weight gain, and food consumption were recorded daily. A caesarean hysterectomy was performed on GD21 to assess the reproductive and foetal outcomes. Exposure to 1000 ppm of xylene caused a significant decrease in the maternal body weight and food consumption, and an increase in intrauterine foetal deaths. Foetal assessment revealed a significant decrease in foetal weight in both male and female foetuses of female rats treated with 500 and 1000 ppm. Male placental weight was significantly decreased in all xylene-treated groups, while 1000 ppm xylene significantly decreased female placental weight. Histologically, marked uterine inflammatory lesions, fibrosis of the liver and renal tissues, as well as increased placental glycogen content were observed. Immunohistochemistry revealed a significant increase in lipid peroxidation and apoptotic markers. Thus, the foeto-maternal toxicities of xylene have been shown to be mediated by a systemic inflammatory response that exacerbates intrauterine oxidative stress and impairs foeto-placental transfer, leading to an increase in foetal mortality.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"692-710"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142296178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental exposure to lead and cadmium only minimally affects the redox system of the follicular fluid and the outcome of intracytoplasmic sperm injection. 环境中铅和镉的暴露对卵泡液的氧化还原系统和卵胞浆内单精子注射的结果影响很小。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-01 Epub Date: 2024-09-26 DOI: 10.1177/07482337241285103

Katarzyna Olszak-Wąsik, Andrzej Tukiendorf, Aleksandra Kasperczyk, Anita Olejek, Mateusz Zamłyński, Stanisław Horák

{"title":"Environmental exposure to lead and cadmium only minimally affects the redox system of the follicular fluid and the outcome of intracytoplasmic sperm injection.","authors":"Katarzyna Olszak-Wąsik, Andrzej Tukiendorf, Aleksandra Kasperczyk, Anita Olejek, Mateusz Zamłyński, Stanisław Horák","doi":"10.1177/07482337241285103","DOIUrl":"10.1177/07482337241285103","url":null,"abstract":"The purpose of our study was to determine the influence of lead and cadmium in concentrations commonly found in the environment on the redox system of the follicular fluid (FF) and on the results of assisted reproduction. A prospective study of 113 patients with unexplained infertility who qualified for intracytoplasmic sperm injection (ICSI). Patients with moderate or severe endometriosis or poor ovarian reserve were excluded from the study. Biochemical analyses and heavy metal assays of follicular fluid and serum (blood) were followed by statistical analyses of dependencies between lead and cadmium and the components of redox system and results of assisted reproduction. A highly significant linear correlation of lead (Pb) and cadmium (Cd) concentrations in serum and in FF was stated. The number of retrieved oocytes and MII (metaphase II stage) oocytes depended on the malondialdehyde (MDA), catalase (CAT), catalase/g of protein (CAT/g of protein), and glutathione reductase (GR) concentrations. Among biochemical factors, MDA was the only factor that correlated negatively with cadmium concentration in serum and FF and simultaneously influenced the number of retrieved oocytes and MII oocytes. The fertilization rate of MII oocytes was influenced by thiol groups-SH, SH/g of protein, CAT, CAT/g of protein, and glutathione peroxidase/g of protein (GPx/g of protein). The Pb and Cd concentrations in FF did not significantly influence the fertilization rates. Lead as well as cadmium at concentrations commonly found in women of reproductive age despite some adaptive changes in the redox system in follicular fluid do not cause large changes in the ovarian follicular environment as a whole and do not significantly worsen the final results of assisted reproduction.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"679-691"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142354439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of genetic polymorphism for detecting genotoxicity in workers occupationally exposed to formaldehyde: A systematic review. 基因多态性对检测职业接触甲醛工人的遗传毒性的影响：系统综述。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-01 Epub Date: 2024-09-08 DOI: 10.1177/07482337241279894

Thiago Guedes Pinto, Ana Claudia Muniz Renno, Patricia Ramos Cury, Daniel Araki Ribeiro

{"title":"The impact of genetic polymorphism for detecting genotoxicity in workers occupationally exposed to formaldehyde: A systematic review.","authors":"Thiago Guedes Pinto, Ana Claudia Muniz Renno, Patricia Ramos Cury, Daniel Araki Ribeiro","doi":"10.1177/07482337241279894","DOIUrl":"10.1177/07482337241279894","url":null,"abstract":"Formaldehyde is a chemical compound capable of preserving cells and tissue morphology, being extensively used worldwide in industrial and medical processes. However, due to the many biological effects that take place after an individual is chronically exposed to formaldehyde, this compound poses a greater cancer risk for workers under its occupational exposure, even at lower concentrations. Thus, the present systematic review aimed to understand whether there may be a positive relation between polymorphism (in terms of individual susceptibility) and genotoxicity in individuals occupationally exposed to formaldehyde. For this purpose, a total of eight selected studies were carefully analyzed by two reviewers, who attributed scores to each study according to the used analysis parameters. First, all studies investigated either pathologists under formaldehyde exposure or anatomical laboratory pathology workers. In addition, the majority of studies were categorized as moderate or strong in the quality assessment. The results revealed a positive association between some polymorphism and genotoxicity in individuals exposed to formaldehyde, since more than half of the studies observed positive relations between genotoxicity and polymorphisms in xenobiotics metabolizing genes. We understand such parameters influence individuals' susceptibility to genomic damage induced by formaldehyde in peripheral blood. In conclusion, individuals with certain genotypes may show higher or lower DNA damage and/or lower or higher DNA repair potential.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"643-652"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142155001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occupational exposure to arsenic and leukopenia risk: Toxicological alert. 职业接触砷与白细胞减少症风险：毒理学警报。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-01 Epub Date: 2024-09-02 DOI: 10.1177/07482337241277261

Nadielle Silva Bidu, Diogo Sousa Lemos, Bruno José Dumêt Fernandes

{"title":"Occupational exposure to arsenic and leukopenia risk: Toxicological alert.","authors":"Nadielle Silva Bidu, Diogo Sousa Lemos, Bruno José Dumêt Fernandes","doi":"10.1177/07482337241277261","DOIUrl":"10.1177/07482337241277261","url":null,"abstract":"Arsenic and its inorganic compounds affect numerous organs and systemic functions, such as the nervous and hematopoietic systems, liver, kidneys, and skin. Despite a large number of studies on arsenic toxicity, rare reports have investigated the leukopenia incidence in workers exposed to arsenic. In workplaces, the main source of workers' exposure is the contaminated air by the inorganic arsenic in mines, arsenic or copper smelter industries, and chemical factories. Erythropoiesis inhibition is one of the arsenic effects and it is related to regulatory factor GATA-1. This factor is necessary for the normal differentiation of early erythroid progenitors. JAK-STAT is an important intracellular signal transduction pathway responsible for the mediating normal functions of several cytokines related to cell proliferation and hematopoietic systems development and regulation. Arsenic inactivates JAK-STAT by inhibiting JAK tyrosine kinase and using the IFNγ pathway. The intravascular hemolysis starts after the absorption phase when arsenic binds to the globin of hemoglobin in erythrocytes and is transported into the body, which increases the oxidation of sulfhydryl groups in hemoglobin. So, this article intends to highlight the potential leukopenia risk via inhalation for workers exposed to arsenic and suggests a possible mechanism for this leukopenia through the JAK-signal transducer and activator of transcription (STAT) pathway inhibition.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"637-642"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perfluorooctane sulfonate causes damage to L-02 cells via Wnt/β-catenin signal path and endoplasmic reticulum stress pathway. 全氟辛烷磺酸通过 Wnt/β-catenin 信号途径和内质网应激途径对 L-02 细胞造成损伤。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-12-01 Epub Date: 2024-08-31 DOI: 10.1177/07482337241277259

Cui Jiajing, Yan Shuqi, Ma Haoyan, Wang Pingwei, Liu Dongge, Liu Yanping, Chen Qianqian, Fajrin Saleh, Ren Shuping

{"title":"Perfluorooctane sulfonate causes damage to L-02 cells via Wnt/β-catenin signal path and endoplasmic reticulum stress pathway.","authors":"Cui Jiajing, Yan Shuqi, Ma Haoyan, Wang Pingwei, Liu Dongge, Liu Yanping, Chen Qianqian, Fajrin Saleh, Ren Shuping","doi":"10.1177/07482337241277259","DOIUrl":"10.1177/07482337241277259","url":null,"abstract":"Perfluorooctane sulfonate (PFOS) is one of the most widely used perfluorinated compounds, and as an environmental endocrine disruptor and environmental persistent pollutant, the threat of PFOS to human health is of increasing concern. Exposure to PFOS has been shown to be closely associated with liver disease, but the intrinsic molecular targets and mechanisms of PFOS-induced liver damage are not well understood. This study was conducted to explore whether the Wnt/β-Catenin signaling pathway and the endoplasmic reticulum stress signaling pathway are involved in damage of PFOS to the liver. In this study, we used the CCK-8 method to detect cell viability, a microscope and DAPI staining to observe cell morphology, flow cytometry to detect cell ROS and apoptosis levels; and Western blot to detect the expressions of proteins in the WNT/β-Catenin, endoplasmic reticulum stress and apoptosis-related pathways. We found that PFOS activated WNT/β-Catenin and endoplasmic reticulum stress-related pathways in L-02 cells and could lead to the development of oxidative stress and apoptosis. Our findings showed that PFOS could cause damage to L-02 cells, and the WNT/β-Catenin signaling and endoplasmic reticulum stress pathways were involved in the changes caused by PFOS to L-02 cells, which provided a new theoretical basis for studying the hepatotoxicity and mechanism of PFOS. PFOS can lead to increased intracellular ROS levels, causing oxidative stress, endoplasmic reticulum stress and activation of the WNT/β-catenin signaling pathway. Our experimental results showed that PFOS can cause damage to L-02 cells, and the WNT/β-Catenin signaling pathway and endoplasmic reticulum stress pathway are involved in the process of damage caused by PFOS to L-02 cells.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"653-666"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142112248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic aluminium chloride exposure induces redox imbalance, metabolic distress, DNA damage, and histopathologic alterations in Wistar rat liver. 长期接触氯化铝会导致 Wistar 大鼠肝脏氧化还原失衡、代谢紊乱、DNA 损伤和组织病理学改变。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2024-11-01 Epub Date: 2024-08-13 DOI: 10.1177/07482337241269784

Farha Shahabuddin, Samina Naseem, Tauseef Alam, Aijaz Ahmed Khan, Farah Khan

{"title":"Chronic aluminium chloride exposure induces redox imbalance, metabolic distress, DNA damage, and histopathologic alterations in Wistar rat liver.","authors":"Farha Shahabuddin, Samina Naseem, Tauseef Alam, Aijaz Ahmed Khan, Farah Khan","doi":"10.1177/07482337241269784","DOIUrl":"10.1177/07482337241269784","url":null,"abstract":"Aluminium, a ubiquitous environmental toxicant, is distinguished for eliciting a broad range of physiological, biochemical, and behavioural alterations in laboratory animals and humans. The present work was conducted to study the functional and structural changes induced by aluminium in rat liver. Twenty five adult male Wistar rats (150-200 g) were randomly divided into five groups; control group and four Al-treated groups viz: Al 1 (25 mg AlCl3/kg b.wt), Al 2 (35 mg AlCl3/kg b.wt), Al 3 (45 mg AlCl3/kg b.wt), and Al 4 (55 mg AlCl3/kg b.wt). Rats in the aluminium-treated groups were administered AlCl3 for 30 days through oral gavage. Aluminium significantly increased the serum levels of liver function markers (ALT, AST, and ALP), phospholipids, and cholesterol. The activities of hepatocyte membrane (ALP, GGT, and LAP) and carbohydrate metabolic (G6P, F16BP, HK, LDH, MDH, ME, and G6PDH) enzymes were significantly altered by AlCl3 administration. Prolonged Al exposure induced oxidative stress in the liver, as evident by significant hepatocellular DNA damage, increased lipid peroxidation, and decreased non-enzymatic and enzymatic antioxidants. The toxic effects observed in this study were AlCl3 dose-dependent. Histopathological examination of liver sections revealed enlargement of sinusoidal spaces, derangement of the hepatic chord, loss of discrete hepatic cell boundaries, congestion of hepatic sinusoids, and degeneration of hepatocytes in Al-intoxicated rats. In conclusion, aluminium causes severe hepatotoxicity by inhibiting the hepatocyte membrane enzymes and disrupting the liver's energy metabolism and antioxidant defence.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"581-595"},"PeriodicalIF":1.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0