Toxicology and Industrial Health最新文献

Studies on the regulatory role of microRNA-30d in chrysotile-transformed MeT-5A cells. microRNA-30d在温石棉转化的MeT-5A细胞中的调控作用研究。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-07-01 Epub Date: 2025-06-04 DOI: 10.1177/07482337251342903

Junxian Li, Yiting Dai, Hefei Huang, Yichen Zhong, Lijin Zhu

{"title":"Studies on the regulatory role of microRNA-30d in chrysotile-transformed MeT-5A cells.","authors":"Junxian Li, Yiting Dai, Hefei Huang, Yichen Zhong, Lijin Zhu","doi":"10.1177/07482337251342903","DOIUrl":"10.1177/07482337251342903","url":null,"abstract":"Asbestos is classified as a class I carcinogen by the International Agency for Research on Cancer (IARC) because of its propensity to accumulate in the lungs and induce malignant tumors, including lung cancer and malignant mesothelioma. The objective of this study was to examine the inhibitory impact of miR-30d on the proliferation, migration, and invasion of chrysotile-transformed human pleural mesothelial cells (MeT-5A). The asbestos-transformed cell model was constructed using a chrysotile asbestos chronically exposed human pleural mesothelial cell line (MeT-5A). The expression level of miR-30d in the transfected cells was determined by qRT-PCR. Cell viability was assessed by CCK-8 assay. The apoptosis rate was evaluated by flow cytometry. The cell scratch assay and the Transwell assay were used to assess cell migration and invasion ability. It was observed that the expression level of miR-30d in Asb MeT-5A+miR-30d cells transfected with miR-30d mimics was markedly elevated in comparison to that in Asb MeT-5A+miR NC cells. Additionally, the cell viability in Asb MeT-5A+miR-30d cells was significantly diminished, while the level of apoptosis was markedly elevated in comparison to that in Asb MeT-5A+miR NC cells. The relative migration area was significantly lower in the Asb MeT-5A+miR-30d group than in the Asb MeT-5A+miR NC group. Furthermore, the number of migrated and invaded cells in the Asb MeT-5A+miR-30d group was significantly less than in the Asb MeT-5A+miR NC group. The findings suggest that miR-30d may suppress the proliferation, apoptosis, migration, and invasion of chrysotile-transformed pleural mesothelial cells.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"365-373"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144216962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neurotoxicity study of copper oxide nanoparticles and the protective role of a probiotic (Lactobacillus acidophilus) in Swiss albino mice. 氧化铜纳米颗粒的神经毒性研究和益生菌（嗜酸乳杆菌）对瑞士白化小鼠的保护作用。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-07-01 Epub Date: 2025-06-10 DOI: 10.1177/07482337251350165

Manisha Sharma, Neelu Kanwar Rajawat

{"title":"Neurotoxicity study of copper oxide nanoparticles and the protective role of a probiotic (Lactobacillus acidophilus) in Swiss albino mice.","authors":"Manisha Sharma, Neelu Kanwar Rajawat","doi":"10.1177/07482337251350165","DOIUrl":"10.1177/07482337251350165","url":null,"abstract":"Nanoparticles (NPs 1-100 nm) play a vital role in medicine, food, and agriculture owing to their unique reactivity and size-dependent optical properties. There are growing concerns about health risks from exposure to engineered NPs. Among these, copper oxide nanoparticles (CuONPs) are an area of research because of their unique electronic, optical, and chemical properties. CuONPs can interact with biological systems, causing oxidative stress, inflammation, neurobehavioral changes, and other pathophysiological effects. This study evaluated the ability of a probiotic (Lactobacillus acidophilus) to prevent CuONP-treated neurotoxicity. In the present study, 24 animals were classified into four groups: control, probiotic (Lactobacillus acidophilus 6.42 mg/kg b.wt.), CuONPs-treated (80 mg/kg b.wt.), and co-administered CuONPs (80 mg/kg b.wt.) + Probiotic (6.42 mg/kg b.wt.). Neurotoxicity was assessed through behavioral tests, including open field, exploratory behavior, pole test, and grip strength tests. Levels of key neurotransmitters viz. acetylcholinesterase, dopamine, and serotonin were measured and histopathological analyses were performed. The CuONP-treated group displayed significant behavioral deficits, decreased neurotransmitter levels, and histopathological abnormalities. In contrast, co-administration of probiotic with CuONPs reduced these effects, as observed by normal behavioral parameters and neurotransmitter levels and improved histopathological architecture. These findings suggested that CuONPs caused neurotoxicity at the tested dose, but co-administration of probiotic effectively mitigated this toxicity. Hence, a probiotic is a promising preventative strategy against CuONP-induced neurotoxic effects.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"398-408"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Landscapes, highlights and trending topics of ferroptosis research in the liver: A bibliometric analysis. 肝脏铁下垂研究的景观、亮点和趋势主题：文献计量学分析。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-07-01 Epub Date: 2025-06-12 DOI: 10.1177/07482337251349366

Xiangning Zhou, Jingjing Yu, Tianwei Sun, Ying Wang, Zhenyu Yao, Ling Gao

{"title":"Landscapes, highlights and trending topics of ferroptosis research in the liver: A bibliometric analysis.","authors":"Xiangning Zhou, Jingjing Yu, Tianwei Sun, Ying Wang, Zhenyu Yao, Ling Gao","doi":"10.1177/07482337251349366","DOIUrl":"10.1177/07482337251349366","url":null,"abstract":"Ferroptosis, a form of programmed iron-dependent cell death, is associated with various liver pathologies. Recent studies have increasingly highlighted the crucial role of ferroptosis in liver function. This study aimed to explore the development of ferroptosis in liver diseases through bibliometric analysis. We retrieved the data from the Web of Science (WOS) Core Collection, then imported selected articles into VOSviewer, CiteSpace, and Microsoft Excel for knowledge mapping analysis. A total of 1,236 articles were included in this study, showing a steady increase in publications on ferroptosis in the liver over the past 11 years, with a significant surge in recent years. China emerged as a dominant contributor, accounting for 86% of global publications. Key institutions included Zhejiang University and Sun Yat-sen University. Zhang Feng from Nanjing Medical University was identified as the most prolific author. The journal Frontiers in Pharmacology published the most articles on this topic. High-frequency keywords included \"ferroptosis,\" \"cell death,\" \"hepatocellular carcinoma,\" \"oxidative stress,\" and \"cancer.\" Our analysis provides insight into the leading countries, institutions, authors, and journals and it highlights recent research hotspots. As an emerging research field, studies on ferroptosis in the liver focused primarily on molecular mechanisms, pathways, and treatment strategies. Hepatocellular carcinoma remains a primary focus within ferroptosis research in the liver. In the future, therapies targeting ferroptosis may offer promising avenues for liver disease treatment.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"386-397"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Air quality and health risks in chemical supply shops in southeastern Nigeria. 尼日利亚东南部化学品供应商店的空气质量和健康风险。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-07-01 Epub Date: 2025-06-08 DOI: 10.1177/07482337251349359

Francis Ugochukwu Madu, Emmanuel Iroha Akubugwo, Toochukwu Ekwutosi Ogbulie, Friday Obinwa Uhegbu, Eni-Yimini Solomon Agoro, Oluchi Ulunma Nwosu, Assumpta Ugbonma Ugenyi, Purity Chioma Ononogbo, Miracle Chinwenmeri Madu

{"title":"Air quality and health risks in chemical supply shops in southeastern Nigeria.","authors":"Francis Ugochukwu Madu, Emmanuel Iroha Akubugwo, Toochukwu Ekwutosi Ogbulie, Friday Obinwa Uhegbu, Eni-Yimini Solomon Agoro, Oluchi Ulunma Nwosu, Assumpta Ugbonma Ugenyi, Purity Chioma Ononogbo, Miracle Chinwenmeri Madu","doi":"10.1177/07482337251349359","DOIUrl":"10.1177/07482337251349359","url":null,"abstract":"This study assessed the concentrations of sulfur dioxide (SO2), nitrogen dioxide (NO2), and hydrogen sulfide (H2S) in chemical stores at Ogbete Market, Enugu, and Ogbo-ogwu Market, Onitsha, southeastern Nigeria, during the wet season (June to August 2024). Air quality measurements, taken from six randomly selected stores in each market and a control site (clothing store) located at about 900 m away, revealed that while concentrations of SO2 and NO2 far exceeded public health guidelines (WHO), they remained below Occupational Safety and Health Administration (OSHA) limits. However, chronic low-level exposure, even within OSHA limits, can still lead to respiratory symptoms and reduced work productivity over time, especially in poorly ventilated indoor shops. Sampling was conducted at a height of 2 m above ground to reflect the breathing zone of an average adult, with data collected during morning and afternoon sessions to capture fluctuations in market activity. Using the Aeroqual Series 300 gas monitor, real-time results indicated hazardous conditions in many stores, and exposure factor-adjusted air concentrations (EF-AACs) were calculated to assess actual exposure scenarios. Hazard quotients (HQs) for each pollutant showed a concerning risk of respiratory diseases, particularly from SO2 and NO2 exposure, with multiple stores exhibiting HQ values greater than 1. The findings highlight the urgent need for improved ventilation and stricter regulations in these markets, while also contributing to the limited research on air quality in commercial environments in Nigeria. This study lays the foundation for future interventions aimed at protecting the health of workers in informal markets, advancing public health initiatives in regions with underdeveloped regulatory frameworks.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"374-385"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144249760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is TiO₂ carcinogenic? A toxicological alert. TiO2致癌吗？毒理学警报。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-07-01 Epub Date: 2025-05-21 DOI: 10.1177/07482337251343397

Nadielle Silva Bidu, Paulo Roberto de Veloso Reis, Ricardo David Couto, Bruno Jose Dumêt Fernandes

{"title":"Is TiO2 carcinogenic? A toxicological alert.","authors":"Nadielle Silva Bidu, Paulo Roberto de Veloso Reis, Ricardo David Couto, Bruno Jose Dumêt Fernandes","doi":"10.1177/07482337251343397","DOIUrl":"10.1177/07482337251343397","url":null,"abstract":"Titanium dioxide (TiO2) is a white powder used in toothpaste, pharmaceuticals, sunscreen, food additives, cosmetics, paints, decontaminants, and photocatalysts. It is produced and used in the workplace in fine and ultrafine particle sizes. International organizations set occupational exposure limits for fine and ultrafine TiO2 particles. TiO2 has not been confirmed to have carcinogenicity in humans, with available assessment data being limited and inconclusive owing to nonspecific effects observed from TiO2 inhalation. The purpose of this toxicological alert is to encourage new studies to determine whether TiO2 is a human carcinogen. TiO2's classification as a possible carcinogen was based on laboratory experiments that produced TiO2 particles with 30-60 nm diameters. These studies assessed the potential risk of exposure to TiO2 nanoparticles, which have diverse and atypical characteristics compared to those found in workplace settings. To date, there are no studies with sufficient data on the duration of pulmonary exposure to TiO2 nanoparticles with significantly relevant workplace associations. Extrapolating results from rodent studies with limited parameter controls do not provide an adequate (translational) assessment of the toxicity of TiO2 nanoparticles on the human respiratory system.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"409-413"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Online software for a new risk assessment method in occupational health and safety: RSCAL. 一种新的职业健康安全风险评价方法——RSCAL在线软件。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-05-01 Epub Date: 2025-04-16 DOI: 10.1177/07482337251333164

Fatih Mehmet Ateş, Ömer Doğan

{"title":"Online software for a new risk assessment method in occupational health and safety: RSCAL.","authors":"Fatih Mehmet Ateş, Ömer Doğan","doi":"10.1177/07482337251333164","DOIUrl":"10.1177/07482337251333164","url":null,"abstract":"Risk assessment is necessary for the safety of employees in the workplace. Risk assessment methods contain some deficiencies arising from the persons performing the analysis. In order to partially overcome this situation, a risk assessment method has been developed by the authors by utilizing the data on occupational accidents in Turkey. In the method, Occupational Health and Safety Professionals who will perform the risk assessment can calculate the risk score without giving a numerical value for any hazard and corresponding risk that they identify in the workplace. In the study, a user-friendly online software called RSCAL by the authors, with a web address of https://rscal.com.tr/, which can meet all the above-mentioned requirements and calculate risk scores for workplaces serving in all types of sectors, has been developed. Through this online application, the identified hazard, risk and exposures of the business to be analyzed can be selected; the necessary corrective and preventive actions can be written by the user and then the risk score can be calculated. After that, all hazards specific to the enterprise can be meticulously identified and the risk assessment of the enterprise can be made by calculating the risk scores. This risk assessment can also be printed out in excel file format. In the future, parameters such as the deadline for each hazard, the legal requirement or standard from which the corrective preventive action to be taken arises can be added to the RSCAL calculation tool.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"276-281"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular toxicity of benzene series released from decorative materials. 装饰材料中苯系物的血管毒性。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1177/07482337251340797

Tanliu Zhang, Xiuhong Zhou, Limei Wang, Chengwang Li, Yan Xu, Zenghui Liu

引用次数: 0

Evaluating the effectiveness of personal protective equipment against engineered nanomaterials: A systematic review. 评估个人防护装备对工程纳米材料的有效性：系统综述。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-05-01 Epub Date: 2025-04-30 DOI: 10.1177/07482337251336897

Rezvan Abedinloo, Rouhollah Parvari, Zahra Cheraghi, Mohammad Javad Assari

{"title":"Evaluating the effectiveness of personal protective equipment against engineered nanomaterials: A systematic review.","authors":"Rezvan Abedinloo, Rouhollah Parvari, Zahra Cheraghi, Mohammad Javad Assari","doi":"10.1177/07482337251336897","DOIUrl":"10.1177/07482337251336897","url":null,"abstract":"A key issue related to the development of nanotechnology is the protection of researchers in laboratories and workers in production facilities from exposure to engineered nanoparticles (ENPs). Personal protective equipment (PPE) can only provide protection if it is selected correctly, well maintained, and used properly in all possible exposures. The objective of this review was to systematically evaluate studies that have assessed the effectiveness of PPE against ENPs. The scientific literature search included the electronic databases of Web of Science, Scopus, MEDLINE via PubMed, Cochrane Collaboration Summaries, and NIOSHTIC. According to the PRISMA flow diagram, 24 papers that met the exclusion and inclusion criteria were included in this review. The review results on gloves, masks, and protective clothing revealed that among the common gloves assessed, nitrile 100 gloves were reported as the least suitable protection method against penetration. Also, most masks have low percent penetration. N99 and P100 masks were more effective than N95 masks. The results also indicated that the physical properties of textiles such as fiber diameter, fabric thickness, porosity, air permeability, and pore size are crucial in determining fabric performance against nanomaterials. Most studies have not used a standardized methodology for evaluating the performance of PPE against nanoparticles, highlighting the need for designing and developing a new method. It is essential to develop a precise and valid application guideline based on scientific evidence to assess protection methods for occupational exposure to ENPs.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"327-345"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased activity of acetylcholine esterase as a biomarker of pesticide exposure in female tea plantation workers. 茶园女工乙酰胆碱酯酶活性降低作为农药暴露的生物标志物。

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-05-01 Epub Date: 2025-04-24 DOI: 10.1177/07482337251336580

Shashi Nandar Kumar, Rakhi Kumari, Nawaid Hussain Khan, Noor Saba Khan, Arnab Nayek, Ankita Sahu, Banajit Bastia, Meenakshi Ahluwalia, Sheikh Raisuddin, Arun Kumar Jain

{"title":"Decreased activity of acetylcholine esterase as a biomarker of pesticide exposure in female tea plantation workers.","authors":"Shashi Nandar Kumar, Rakhi Kumari, Nawaid Hussain Khan, Noor Saba Khan, Arnab Nayek, Ankita Sahu, Banajit Bastia, Meenakshi Ahluwalia, Sheikh Raisuddin, Arun Kumar Jain","doi":"10.1177/07482337251336580","DOIUrl":"10.1177/07482337251336580","url":null,"abstract":"Because of their beneficial effects in controlling pests, pesticides are used worldwide to reduce pests in agricultural fields and commercial gardens, thereby increasing the crop yield. Pesticides are ubiquitous in the environment and besides targeting pests they also affect non-target organisms. This study was undertaken to evaluate the activity of acetylcholine esterase (AChE) inhibition and its associated health effects in female tea plantation workers (TPW). In silico analysis was applied to identify whether pesticide exposure had an increased affinity after binding with the AChE enzyme, and the findings were validated by measuring the AChE activity in the plasma of study subjects by the biochemical analysis. The activity of AChE was found to be considerably compromised in TPW exposed to pesticides. Inhibition of AChE activity may lead to severe adverse health effects, such as cough, fatigue, and headache in TPW exposed to pesticides. Among all pesticides, λ-cyhalothrin, fipronil, and fenazaquine had the highest binding affinity with AChE (-10.098 Kcal/mol, -8.574 Kcal/mol, and -8.507 Kcal/mol, respectively) as compared to the other pesticides and their natural acetylcholine substrate (-4.398 Kcal/mol). Based on in silico results, AChE was found to have the highest binding affinity with λ-cyhalothrin, fipronil, and fenazaquine, and these pesticides could be responsible for the enzyme activity inhibition. Hence, these pesticides may cause more adverse health effects on humans compared to other pesticides. This finding on biomarker role for AChE may aid in the development of effective antidotes against pesticide exposure, thereby faciliating mitigation of negative health effects of pesticides.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"294-313"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the mechanism of METTL3-mediated m6A methylation in silicon dust-induced epithelial-mesenchymal transition. mettl3介导的m6A甲基化在硅尘诱导的上皮-间质转化中的机制研究

IF 1.7 4区医学

Toxicology and Industrial Health Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1177/07482337251339926

Gao Yue, Duan Zhizhen, Yu Zhengmin, Shi Xinwei, Wang Ning, Wu Liting, Li Wei, Zhu Baoli, Sun Kai, Han Lei

{"title":"Insights into the mechanism of METTL3-mediated m6A methylation in silicon dust-induced epithelial-mesenchymal transition.","authors":"Gao Yue, Duan Zhizhen, Yu Zhengmin, Shi Xinwei, Wang Ning, Wu Liting, Li Wei, Zhu Baoli, Sun Kai, Han Lei","doi":"10.1177/07482337251339926","DOIUrl":"10.1177/07482337251339926","url":null,"abstract":"Silicosis is an occupational lung disease caused by long-term inhalation of silica dust. Its characteristic pathological manifestation is progressive pulmonary fibrosis. Epithelial-mesenchymal transition (EMT) plays an important role in the occurrence and development of silicosis fibrosis, and N6-methyladenosine (m6A), as an important form of RNA modification, is closely related to the expression and regulation of multiple genes in the process of fibrosis. In the present study, we demonstrated that m6A modification significantly increases during silica-induced EMT. Silencing Methyltransferase Like 3 (METTL3) to reduce m6A modification levels inhibited the EMT process, suggesting that METTL3-mediated m6A modification could be an effective strategy for intervening in pulmonary fibrosis. Subsequently, through RNA sequencing, protein-protein interaction network analysis, and Methylated RNA Immunoprecipitation-RT-qPCR, we identified c-Myc as a downstream target of METTL3. In summary, our findings illuminate the relationship between m6A modification, METTL3, and the progression of silicosis, particularly through the EMT process. These results suggest that targeting METTL3 could be a promising therapeutic approach for modulating m6A levels and intervening in the progression of silicosis.","PeriodicalId":23171,"journal":{"name":"Toxicology and Industrial Health","volume":" ","pages":"314-326"},"PeriodicalIF":1.7,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0